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  • 05. General::05.07. Space and Planetary sciences::05.07.02. Space weather  (2)
  • XFELP-type ATPasesligand screeningserial femtosecond crystallography  (2)
  • protein crystallizationX-ray diffractionserial crystallographymicrofluidic devices  (2)
  • quasicrystalssuperspace crystallographystructure analysisphasonsX-ray diffuse scattering  (2)
  • International Union of Crystallography (IUCr)  (6)
  • American Institute of Physics  (1)
  • ESA (European Space Agency)  (1)
  • Cell Press
Collection
Publisher
Years
  • 1
    Publication Date: 2015-08-19
    Description: The name of one of the authors in the article by Heymann et al. [(2014), IUCrJ, 1, 349–360] is corrected.
    Keywords: protein crystallizationX-ray diffractionserial crystallographymicrofluidic devices
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 2
    Publication Date: 2015-08-16
    Description: An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the supersaturation. This mechanism is exploited to produce one crystal per drop. Diffraction data are measured, one crystal at a time, from a series of room-temperature crystals stored on an X-ray semi-transparent microfluidic chip, and a 93% complete data set is obtained by merging single diffraction frames taken from different unoriented crystals. As proof of concept, the structure of glucose isomerase was solved to 2.1 Å, demonstrating the feasibility of high-throughput serial X-ray crystallography using synchrotron radiation.
    Keywords: protein crystallizationX-ray diffractionserial crystallographymicrofluidic devices
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 3
    Publication Date: 2015-06-13
    Description: Membrane proteins are key players in biological systems, mediating signalling events and the specific transport of e.g. ions and metabolites. Consequently, membrane proteins are targeted by a large number of currently approved drugs. Understanding their functions and molecular mechanisms is greatly dependent on structural information, not least on complexes with functionally or medically important ligands. Structure determination, however, is hampered by the difficulty of obtaining well diffracting, macroscopic crystals. Here, the feasibility of X-ray free-electron-laser-based serial femtosecond crystallography (SFX) for the structure determination of membrane protein–ligand complexes using microcrystals of various native-source and recombinant P-type ATPase complexes is demonstrated. The data reveal the binding sites of a variety of ligands, including lipids and inhibitors such as the hallmark P-type ATPase inhibitor orthovanadate. By analyzing the resolution dependence of ligand densities and overall model qualities, SFX data quality metrics as well as suitable refinement procedures are discussed. Even at relatively low resolution and multiplicity, the identification of ligands can be demonstrated. This makes SFX a useful tool for ligand screening and thus for unravelling the molecular mechanisms of biologically active proteins.
    Keywords: XFELP-type ATPasesligand screeningserial femtosecond crystallography
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 4
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    International Union of Crystallography (IUCr)
    In: IUCrJ
    Publication Date: 2016-06-28
    Keywords: quasicrystalssuperspace crystallographystructure analysisphasonsX-ray diffuse scattering
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 5
    Publication Date: 2016-06-15
    Description: The detailed atomic structure of the binary icosahedral (i) ScZn7.33 quasicrystal has been investigated by means of high-resolution synchrotron single-crystal X-ray diffraction and absolute scale measurements of diffuse scattering. The average atomic structure has been solved using the measured Bragg intensity data based on a six-dimensional model that is isostructural to the i-YbCd5.7 one. The structure is described with a quasiperiodic packing of large Tsai-type rhombic triacontahedron clusters and double Friauf polyhedra (DFP), both resulting from a close-packing of a large (Sc) and a small (Zn) atom. The difference in chemical composition between i-ScZn7.33 and i-YbCd5.7 was found to lie in the icosahedron shell and the DFP where in i-ScZn7.33 chemical disorder occurs on the large atom sites, which induces a significant distortion to the structure units. The intensity in reciprocal space displays a substantial amount of diffuse scattering with anisotropic distribution, located around the strong Bragg peaks, that can be fully interpreted as resulting from phason fluctuations, with a ratio of the phason elastic constants K2/K1 = −0.53, i.e. close to a threefold instability limit. This induces a relatively large perpendicular (or phason) Debye–Waller factor, which explains the vanishing of `high-Qperp' reflections.
    Keywords: quasicrystalssuperspace crystallographystructure analysisphasonsX-ray diffuse scattering
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 6
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    International Union of Crystallography (IUCr)
    In: IUCrJ
    Publication Date: 2015-07-01
    Keywords: XFELP-type ATPasesligand screeningserial femtosecond crystallography
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 7
    Publication Date: 2017-04-04
    Description: COST (Co-operation in the field of Scientific and Technical Research) is an important instrument supporting co-operation among scientists and researchers across Europe now joining 35 member countries. Scientific projects in the COST framework are called COST Actions and have the objectives embodied in their respective Memorandum of Understanding (MoU). The main objectives of the COST Actions within the European ionospheric and radio propagation community have been: to study the influence of upper atmospheric conditions on terrestrial and Earthspace communications, to develop methods and techniques to improve existing and generate new ionospheric and propagation models over Europe for telecommunication and navigation applications and to transfer the results to the appropriate national and international organizations, institutions and industry dealing with the modern communication systems. This paper summarises in brief the background and historical context of four ionospheric COST Actions and outlines their main objectives and results. In addition, the paper discusses the dissemination of the results and the collaboration among the participating institutions and researchers.
    Description: DRS Codem Systems Ball Aerospace Corporation University of Massachusetts Lowell
    Description: Published
    Description: Lowell, Massachusetts, U.S.A., April 29, 2007
    Description: 3.9. Fisica della magnetosfera, ionosfera e meteorologia spaziale
    Description: open
    Keywords: Physics of the Ionosphere ; 01. Atmosphere::01.02. Ionosphere::01.02.99. General or miscellaneous ; 05. General::05.07. Space and Planetary sciences::05.07.02. Space weather
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Conference paper
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  • 8
    Publication Date: 2017-04-04
    Description: We analyse GNSS ionospheric scintillation data in the polar areas of both hemispheres to develop a climatology over a large geomagnetic quiet period. The conditions of the near-Earth environment leading to scintillation scenarios are investigated via scintillation occurrence. Within this scope we realize maps of scintillation occurrence as a function of the magnetic local time(MLT) and of the altitude adjusted corrected geomagnetic coordinates (AACGM). The maps are realized merging observations from a network of four GISTM (GPS Ionospheric Scintillation and TEC Monitor) in the Northern Europe and two GISTM in Antarctica during the year 2008. The results highlight the possibility to investigate the impact of ionospheric irregularities on the phase and amplitude of GNSS signals, evidencing the auroral and cusp/cap contributions. This works aims to contribute to development of nowcasting and forecasting tools for GNSS ionospheric scintillation.
    Description: Published
    Description: Università di Padova
    Description: 3.9. Fisica della magnetosfera, ionosfera e meteorologia spaziale
    Description: open
    Keywords: Ionospheric irregularities ; Polar Cap phenomena ; Space and satellite communication ; 01. Atmosphere::01.02. Ionosphere::01.02.07. Scintillations ; 05. General::05.07. Space and Planetary sciences::05.07.01. Solar-terrestrial interaction ; 05. General::05.07. Space and Planetary sciences::05.07.02. Space weather
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: Extended abstract
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