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  • Environmental sensing
  • Lipids
  • Wiley-Blackwell  (18)
  • INSTM  (3)
  • American Chemical Society  (2)
  • Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu  (1)
Collection
  • 1
    Publication Date: 2021-05-19
    Description: La boutargue est constituée par les ovaires salés et séchés des mulets à grosse tête (Mugil cephalus) et de la courbine (Argyrosomus regius) représente une ressource importante pour la région de Nouakchott et pour la Mauritanie. Pour déterminer la valeur énergétique dans les boutargues, nous avons effectué des dosages des protéines, des glucides, des lipides et des études analytiques des acides gras et des lipides. Les analyses ont révélé que la boutargue contient, par rapport aux autres éléments d’énergie, très peu de glucide : 3,29% dans la boutargue du mulet et 1,95% dans celle de la courbine. Alors que les teneurs en protéines et lipides sont importantes et sont respectivement de 44,44% et 31,68% dans la boutargue du mulet et de 55,11% et 15,86% dans celle de la courbine. La boutargue est d’une grande valeur nutritionnelle. En effet, la boutargue du mulet contient 493,82 kcal/100g, alors que celle de la courbine contient 384,27 kcal/100g. En outre, chaque gramme de boutargue du mulet contient 28,1mg d’acides gras, tandis que celle de la courbine contient 19,7mg. Les acides gras trouvés dans les boutargues étudiées sont subdivisés en 3 groupes: - Les acides gras saturés : 17,9% dans la boutargue du mulet et 1,8% dans celle de la courbine, - Les acides gras monoinsaturés : 39,4% dans la boutargue du mulet et 40,65% dans celle de la courbine, - Les acides gras polyinsaturés : 28,8% pour la boutargue du mulet et 35,89 % dans celle de la courbine. La quantité lipidique du mulet est supérieure à celle de la courbine, mais la quantité de protéine est inférieure. Le taux d’énergie de la boutargue du mulet est supérieur à celui de la courbine. Néanmoins, cette dernière contient plus d’acides gras mono et poly insaturés que celle du mulet.
    Description: تمثل البطارخ، مبايض سمك البوري (Mugil cephalus) والكوربين (Argyrosomus regius) المملحة و المجففة، ثروة هامة لمنطقة نواكشوط ولموريتانيا. لمعرفة مقدار الطاقة في هذه البطارخ، قمنا بدراسة كمية لمكوناتها الطاقية : الدهنيات والبروتينات و السكريات و كيفية للدهنيات. بينت هذه الدراسة أن البطارخ تحتوي على نسب ضئيلة من السكريات. ففي بطارخ الكوربين لاتتجاوز 1,95 % و3,29 %في بطارخ البوري. في هذه الأخيرة ترتفع نسبتي البروتينات و الدهنيات إلى 44,44 % و31,68 % بينما في بطارخ الكوربين تصل هاتين النسبتين إلى%55,11 و15,86 %. كما تبين أن البطارخ غنية بالطاقة حيث توجد بها 493,80 كيلوكالوري/100غ في بطارخ البوري و 384,27 كيلوكالوري/100غ في بطارح الكوربين وكل غرام من بطارخ البوري تحتوي على 28,1 ملغ أحماض دهنية و19,7 أحماض دهنية ببطارخ الكوربين . تنقسم هذه الأحماض إلى ثلاث مجموعات أساسية مختلفة كما وكيفا وهي: - الأحماض الدهنية المشبعة17,9 : % ببطارخ البوري و1,8 % ببطارخ الكوربين. - الأحماض الدهنية أحادية عدم التشبع : % 39,4 ببطارخ البوري و 40,65 % ببطارخ الكوربين. - الأحماض الدهنية متعددة عدم التشبع : 28,8 % ببطارخ البوري و35,89 % ببطارخ الكوربين. نستنتج أن كمية الدهنيات ببطارخ البوري أكثر من نظيرتها بالكوربين، بينما نرى العكس بالنسبة لكمية البروتينات حيث نرى أنها أكثر ببطارخ الكوربين أما كمية الطاقة فتبدو أعلى ببطارخ البوري من بطارخ الكوربين كما تحتوي هذه الأخيرة على أحسن نوعية للدهنيات.
    Description: The boutargue is consisted of salted and dried ovaries of the mullet (Mugil cephalus) and black umber (Argyrosomus regius) is an important resource for the region of Nouakchott and Mauritania. In order to determine the energetic value of the boutargue, we measured the proteins, the carbohydrates and the lipids contents, in addition to the analytical studies of the fatty acids. These experiments revealed that the boutargue contains very low carbohydrate levels: 3,29% for the mullet and 1,95% for the black umber. The protein and the lipid contents were 44,44% and 31,68% respectively for the mullet while, they were respectively 55,11% and 15,86% for the black umber. This study showed the good nutritional quality of boutargue. In fact, the boutargue of the mullet contains 493,82kcal/100g, where as that of the black umber contains 384,27kcal/100g. Each gram of boutargue of the mullet contains 28,1mg of fatty acids and while that of the black umber contain 19,7mg of fatty acids. The fatty acids found in the studied boutargues were subdivided in three groups: - Saturated fatty acids: 17,9% for the mullet and 1,8% for the black umber, - Unsaturated mono fatty acids: 39,4% for the mullet and 40,65% for the black umber, - Polyunsaturated fatty acids: 28,8% for the mullet and 35,89 % for the black umber, As compared to the black umber's boutargue, the mullet boutargue contains high lipid quantity. Where as, the quantity of protein in the black umber’s boutargue was higher as compared to the mullet's boutargue. The energetic rate of the mullet boutargue is higher than that of the black umber. But the black umber boutargue contains more polyunsaturated and unsaturated mono fatty acids.
    Description: Published
    Description: Argyrosomus regius, Mugil cephalus
    Keywords: Carbohydrates ; Chemical composition ; Fatty acids ; Nutritive value ; Ovaries ; Argyrosomus regius ; Lipids ; Proteins ; Carbohydrates ; Chemical composition ; Fatty acids ; Food fish ; Lipids ; Nutritive value ; Ovaries ; Proteins
    Repository Name: AquaDocs
    Type: Journal Contribution , Non-Refereed , Article
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  • 2
    Publication Date: 2021-05-19
    Description: لدراسة تأثير عملية تحويل سمك السردين واللاطشة إلى المعلبات على الجودة الغذائية، قمنا بمتابعة موشرات جودة الدهنيات و تركيبتها من الأحماض الدهنية و أثبتت النتائج أن تغيرات كمية و جودة الدهنيات متعلقة أساسا بتركيبة سمك السردين أو اللاطشة الطازج بالدهنيات و بتركيبة المادة الحافظة (زيت الزيتون أو الطماطم). كما أثبتت التحاليل بواسطة الكروماتوغرافيا ذات الطور الغازي ثراء المعلبات بالأحماض الدهنية التالية C18 :1 ω9 وC18 :2 ω6 . وأخيرا، يمكننا التأكيد أن سمك السردين واللاطشة المعلب بزيت الزيتون و الطماطم غني بالأحماض الدهنية من عائلتيωω3 وω6 .
    Description: The effects of cooking and sterilisation steps on muscle lipid deterioration of Sardinella aurita and Sardina pilchardus canned in olive oil and tomato sauce respectively were investigated. Lipid contents of Sardinella aurita flesh were significantly affected (p 〈 0.05) by the canning process. However, lipid levels of Sardina pilchardus remained constant during processing. The peroxide value and thiobarbituric index increased slightly after the cooking step but changed significantly (p 〈 0.05) following canning. Both canned sardine and sardinella absorbed coating-oil during sterilisation inducing a higher oleic (C18:1 n-9) and linoleic (C18:2 n-6) acids content. Independently of coating-oil category, the eicosapentaenoic (C20:5 n-3) and docosahexaenoic (C22:6 n-3) acids concentrations ranged from 3.00 to 6.24% and from 5.12% to 40.26% respectively. Although, lipids of Sardinella aurita and Sardina pilchardus were slightly affected by the canning process, and they remain a good source of ω3 and ω6 fatty acids.
    Description: Dans le présent travail, les effets de la cuisson et de la stérilisation sur la qualité et la composition en acide gras des lipides de la sardinelle Sardinella aurita et la sardine Sardina pilchardus au cours de la mise en boite dans l’huile d’olive et la sauce de tomate ont été étudiés. La teneur en lipides dans la chair de la sardinelle Sardinella aurita a augmenté significativement (p 〈0.05) après la mise en boite. Cependant, celle de la sardine Sardina pilchardus a demeuré stable dans les mêmes conditions. D’autre part, l’étude des indicateurs d’altération des lipides nous a montré que la qualité des lipides de la sardine est affectée par la stérilisation mais pas par la cuisson. La composition en acides gras de la sardine et la sardinelle en boite de conserve change significativement après stérilisation. En effet, indépendamment de la nature d’huile conservatrice, les lipides de la matière première s’enrichissent en acide oléique (C18:1 n-9) et linoléique (C18:2 n-6). Les lipides de la sardine et la sardinelle en boite représentent une source en acides gras poly insaturés de la familles des ω3 et ω6. Mots clés: Mise en boite; Sardinella aurita; Sardina pilchardus; huile d’olive; Sauce tomate; Acide gras
    Description: Published
    Keywords: Sardina pilchardus ; Sardinella aurita ; Oxidation ; Canning ; Lipids ; Polyunsaturated fatty acids ; Canning ; Lipids ; Oxidation ; Polyunsaturated fatty acids ; Processing fishery products ; Quality control
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
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  • 3
    Publication Date: 2021-05-19
    Description: The boutargue is the salty and dried ovary of the mullet (Mugil cephalus) from Nouakchott (Mauritania) and Tunis (Tunisia). In order to know the nutritional quality of the boutargue, we have used an analytic analysis and dosages of lipids and their fat acids. For this study, we used two different methods of extraction of the lipids: method of Folch modified by Bligh and Dyer and the method of Soxhlet. These experiences show that the boutargue of mullet is rich in lipids, 30.9% in Nouakchott (Atlantic Ocean) and 27,4% in Tunis (Mediterranean Sea). The boutargue is with good nutritional quality. Indeed, each gram of boutargue contains 28,1 mg of fatty acids in Nouakchott and 19,7 mg in Tunisia. Theses fatty acids are subdivide in 3 essential groups: - Fatty acid saturated 17,9% in Atlantic Ocean and 2,8% in Mediterranean Sea. - Fatty acid monounsaturated 39, 4% in Atlantic Ocean and 41,4% in Mediterranean Sea. - Fatty acids polyunsaturated 28, 8% in Atlantic Ocean and 39 % in Mediterranean Sea. The quantity of lipids in Atlantic Ocean boutargue is superior to Mediterranean Sea boutargue but the quality of lipids in Mediterranean Sea boutargue is superior to Atlantic Ocean boutargue
    Description: تمثل البطارخ بيض سمك البوري (Mugil cephalus) المملحة و المجففة لمنطقتي نواكشوط (موريتانيا) و تونس (تونس). لمعرفة الجوانب البيوكيميائية و الغذائية لهذه البطارخ، قمنا بدراسة تحليلية كمية و كيفية للدهنيات. فاستخدمنا طريقتين مختلفتين لاستخلاص الدهون، هما طريقة فولش المعدلة من طرف بليغر ودير و طريقة سوكسلي. تبين أن البطارخ غنية بالدهنيات30,9 % بمنطقة نواكشوط (المحيط الأطلسي) و27,4 % بمنطقة تونس (البحر الأبيض المتوسط). يحتوي كل غرام من بطارخ منطقة نواكشوط على 28,1 ملغ من الأحماض الدهنية بينما لا تتعدى هذه النسبة 19,7 ملغ من الأحماض الدهنية ببطارخ منطقة تونس. تنقسم هذه الأحماض الى ثلاث مجموعات أساسية مختلفة كما وكيفا وهي: - الأحماض الدهنية المشبعة تصل إلى17,9 % ببطارخ المحيط الأطلسي بينما لا تتجاوز2,8 % ببطارخ البحر الأبيض المتوسط. - الأحماض الدهنية أحادية عدم التشبع بنسبة تصل إلى %39,4 ببطارخ المحيط الأطلسي بينما ترتفع هذه النسبة إلى 41,4 % ببطارخ البحر الأبيض المتوسط. - الأحماض الدهنية متعددة عدم التشبع بنسبة تصل إلى 28,8 % ببطارخ المحيط الأطلسي بينما ترتفع هذه النسبة إلى39 %.ببطارخ البحر الأبيض المتوسط. لقد بينت هذه الدراسة أن بطارخ المحيط الأطلسي﴿ نواكشوط- موريتانيا﴾، توجد بها نسبة من الدهنيات أكثر من نظيرتها بالبحر الأبيض المتوسط ﴿تونس- تونس﴾، بينما تمتاز هذه الأخيرة ﴿بطارخ البحر الأبيض المتوسط﴾ بجودة أكثر لدهنياتها بالمقارنة مع الأولى ﴿ بطارخ المحيط الأطلسي﴾.
    Description: La boutargue est constituée par les ovaires salés et séchés des mulets à grosse tête (Mugil cephalus) de la région de Nouakchott (Mauritanie) et de la région de Tunis (Tunisie). Pour savoir la quantité et la qualité de lipides dans cette boutargue nous avons effectué des études analytiques et des dosages des lipides et des acides gras de ceux-ci. Nous avons procédé par deux méthodes d’extraction des lipides : la méthode de Folch modifiée par Bligh et Dyer et la méthode de Soxhlet . Ces expériences ont révélé que la boutargue est très riche en lipides : 30,9% dans la région de Nouakchott (Océan Atlantique) et 27,4% dans la région de Tunis (Mer Méditerranéenne) qu’il est de bonne qualité nutritionnelle et que chaque gramme de boutargue contient 28,1 mg des acides gras dans la région de Nouakchott et 19,7 mg dans la région de Tunis. Ils sont subdivisés en 3 groupes essentiels : - acides gras saturés 17,9% dans l’Océan Atlantique et 2,8% dans la Mer Méditerranéenne. - acides gras mono insaturés 39,4 % dans l’Océan Atlantique et 41,4% dans la Mer Méditerranée. - acides gras polyinsaturés 28,8% dans l’Océan Atlantique et 39 % dans la Mer Méditerranée. La quantité lipidique de la boutargue atlantique est supérieure à celle de la Méditerranée et la qualité de la boutargue Méditerrané est meilleure que celle de l’Atlantique.
    Description: Published
    Keywords: Boutargue ; Mauritania ; Tunisia ; Mugil cephalus ; Lipides ; Acides gras ; Mulet ; Fatty acids ; Nutritive value ; Lipids ; Comparative studies ; Fatty acids ; Lipids ; Nutritive value ; Dried fish
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed , Article
    Format: 270212 bytes
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  • 4
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: Coral physiological and δ15N isotopic measurements.
    Description: Experimental coral physiological and δ15N isotopic measurements in October 2012 at Reef Systems Coral Farm, Ohio. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/839920
    Description: NSF Emerging Frontiers Division (NSF EF) EF-1041124
    Keywords: Coral calcification ; Photosynthesis ; Carbon budgets ; Chlorophyll a ; Protein ; Carbohydrates ; Lipids ; Energy reserves ; Nitrogen isotopes
    Repository Name: Woods Hole Open Access Server
    Type: Dataset
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  • 5
    Publication Date: 2022-05-27
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Gosselin, K. M., Nelson, R. K., Spivak, A. C., Sylva, S. P., Van Mooy, B. A. S., Aeppli, C., Sharpless, C. M., O’Neil, G. W., Arrington, E. C., Reddy, C. M., & Valentine, D. L. Production of two highly abundant 2-methyl-branched fatty acids by blooms of the globally significant marine cyanobacteria Trichodesmium erythraeum. ACS Omega, 6(35), (2021): 22803–22810, https://doi.org/10.1021/acsomega.1c03196.
    Description: The bloom-forming cyanobacteria Trichodesmium contribute up to 30% to the total fixed nitrogen in the global oceans and thereby drive substantial productivity. On an expedition in the Gulf of Mexico, we observed and sampled surface slicks, some of which included dense blooms of Trichodesmium erythraeum. These bloom samples contained abundant and atypical free fatty acids, identified here as 2-methyldecanoic acid and 2-methyldodecanoic acid. The high abundance and unusual branching pattern of these compounds suggest that they may play a specific role in this globally important organism.
    Description: This work was funded with grants from the National Science Foundation grants OCE-1333148, OCE-1333162, and OCE-1756254 and the Woods Hole Oceanographic Institution (IR&D). GCxGC analysis made possible by WHOI’s Investment in Science Fund.
    Keywords: Lipids ; Alkyls ; Bacteria ; Genetics ; Chromatography
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 6
    Publication Date: 2022-10-21
    Description: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Colson, B. C., & Michel, A. P. M. Flow-through quantification of microplastics using impedance spectroscopy. ACS Sensors, 6(1), (2021): 238–244, doi:10.1021/acssensors.0c02223.
    Description: Understanding the sources, impacts, and fate of microplastics in the environment is critical for assessing the potential risks of these anthropogenic particles. However, our ability to quantify and identify microplastics in aquatic ecosystems is limited by the lack of rapid techniques that do not require visual sorting or preprocessing. Here, we demonstrate the use of impedance spectroscopy for high-throughput flow-through microplastic quantification, with the goal of rapid measurement of microplastic concentration and size. Impedance spectroscopy characterizes the electrical properties of individual particles directly in the flow of water, allowing for simultaneous sizing and material identification. To demonstrate the technique, spike and recovery experiments were conducted in tap water with 212–1000 μm polyethylene beads in six size ranges and a variety of similarly sized biological materials. Microplastics were reliably detected, sized, and differentiated from biological materials via their electrical properties at an average flow rate of 103 ± 8 mL/min. The recovery rate was ≥90% for microplastics in the 300–1000 μm size range, and the false positive rate for the misidentification of the biological material as plastic was 1%. Impedance spectroscopy allowed for the identification of microplastics directly in water without visual sorting or filtration, demonstrating its use for flow-through sensing.
    Description: The authors thank the Richard Saltonstall Charitable Foundation and the National Academies Keck Futures Initiative (NAKFI DBS13) for their funding support.
    Keywords: Microplastics ; Plastics ; Impedance spectroscopy ; Dielectric properties ; Instrumentation ; Particle detection ; Flow-through ; Environmental sensing
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 7
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 6 (1983), S. 575-576 
    ISSN: 0935-6304
    Keywords: Thin-film chromatography, HPTFC ; Separation on argentized silica gel ; Detection ; Scanning densitometry ; Lipids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1995 (1995), S. 267-277 
    ISSN: 0947-3440
    Keywords: Carbohydrates ; Pseudosugars ; Cyclitols ; Glycoceramide analogs ; Lipids ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Carbocyclic analogs of glycoceramides, (2S,3R,4E)-1-(5a-carba-β-D-glycopyranosyl)-2-(hexadecanoylamino)-4-octa-decen-3-ols E-3-E-6, linked by imino, ether and sulfide bridges, were synthesized by coupling of aziridines, as the sphingosine precursors, with protected 1-amino, 1-hydroxy and 1-mercapto derivatives of 5a-carba sugars and subsequent deprotection and N-acylation. Biological assay of 5a-carbaglycosylceramides showed that both imino-linked E-3 and E-4 having gluco and galacto configurations are mild immunomodulators and possess a mild inhibitory activity against gluco- and galactocerebrosidases. These findings prompted us to prepare the corresponding Z-isomers Z-3 and Z-4, which interestingly show a similar enzyme-inhibitory activity.
    Additional Material: 1 Tab.
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  • 9
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 182-185 
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography ; Lipids ; Bacterial glycerol tetraethers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The analysis of bacterial glycerol tetraethers by capillary supercritical fluid chromatography is described. Complete separation of ethers, differing only by the number of cyclopentane rings present in the isopranyl side chains could be achieved. On a 50% methylphenylpolysiloxane coated capillary, the ethers eluted in the order of increasing cyclopentane content. The analysis revealed the presence of two previously unreported structural isomers of glycerol tetraethers. The method can be used qualitatively and quantitatively as a fast and sensitive screening test for such compounds in living organisms and sedimentary organic matter.
    Additional Material: 2 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 24 (1993), S. 400-422 
    ISSN: 1059-910X
    Keywords: Cryofixation ; Freeze substitution ; Low temperature embedding ; Lowicryl ; Fine structural preservation ; Extracellular material ; Bacteria ; Algae ; Cyanobacteria ; Fungi ; Transgenic plants ; Wheat ; Barley ; Tobacco ; Conidia ; Hyphae ; Haustoria ; Plant pathogen interface ; Molecular preservation ; Retention diffusible elements ; Microanalysis ; Autoradiography ; Fungicides ; Lipids ; Immunocytochemistry ; Protein antigenicity ; Elicitor ; Extensin ; Foreign proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Considering the increasing necessity for improved preparation techniques in biological electron microscopy as a basis for the identification and localization of cellular substances within the compartments of the cell, this review is focussed on the method of freeze substitution as an important link between the cryofixation (ultrarapid freezing) and resin embedding of biological specimens. The theory and practice of freeze substitution is summarized with particular interest in the physical and thermodynamic as well as in the chemical basis of this technique. A survey of practical aspects of the technical process of freeze substitution concerning the equipment and various protocols successfully applied in biological systems is also given. The main advantage of freeze substitution versus conventional chemical fixation is seen in the maintenance of the hydration shell of molecules and macromolecular structures. This results in an improved fine structural preservation, superior retention of the antigenicity of proteins and decreased loss of unbound, diffusible cellular components. Examples of excellent visualization of the ultrastructure of macro-molecular complexes (nucleic acids, extracellular material, membranes etc.), small organisms (bacteria, algae, cyanobacteria and fungi) and large biological samples such as plant and animal tissue as well as the plant-pathogen (fungus) interface and infection structures are presented. Recent data on the molecular characterization of freeze-substituted biological tissue are exemplified with special emphasis on the subcellular detection of soluble components (elements, lipids, proteins and drugs) and the inter-/intracellular localization of proteins including foreign proteins in transgenic plants. The molecular analysis of freeze-substituted specimens is achieved by the combination of low temperature preparation techniques in biological electron microscopy with various detection methods such as X-ray microanalysis, immunocytochemistry and high resolution autoradiography. © 1993 Wiley-Liss, Inc.
    Additional Material: 27 Ill.
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