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  • eLife Sciences Publications  (6)
  • Blackwell Publishing Ltd  (5)
  • MDPI  (4)
  • Oxford University Press  (4)
  • 1
    Electronic Resource
    Electronic Resource
    A Theoretical Framework Linking Creativity, Empowerment, and Organizational Memory (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Creativity and innovation management 6 (1997), S. 0 
    Details
    ISSN: 1467-8691
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
    Notes: Empowerment, creativity, and organizational memory are constructs that have been researched in MIS. While each construct has received individual attention, we have found relatively little research linking them. One of the major edicts of empowerment is delegation of decision making authority to lower-level employees. Increased authority allows employees more freedom to be creative. However, if creative thought is generated but not captured, innovative ideas may be lost. Organizational memory can capture creative ideas as they are generated so that empowered teams can draw upon positive creative experiences.We developed a theoretical model to illuminate the relationships between organizational memory, worker empowerment, and creativity. The model portrays the linkages between empowerment and creativity, creativity and organizational memory, and organizational memory and empowerment. The model was developed based on the literature in each respective area and an interview-based study concerning “empowered” systems development project teams and organizational memory. Analysis of the interview data revealed that empowered workers generate creative solutions to problems. However, creative solutions can only be used for future projects if they are somehow recorded into organizational memory. Organizations that empowered their workforce and embraced creativity reported increased customer satisfaction, waste reduction, and some quality gains. In contrast, those that did not empower reported little or no change. Organizations that recorded creative solutions to problems believe that retrieval of this information could be potentially useful for future projects. Potential challenges faced by organizations classified into each cell are also presented. This classification scheme should prove useful as a guide to organizations examining the potential benefits and pitfalls of worker empowerment and organizational memory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/1467-8691.00047
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  • 2
    Electronic Resource
    Electronic Resource
    Estimation of Solute Concentrations Using the Pathline Counting Method (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 32 (1994), S. 0 
    Details
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The paper discusses the use of the “pathline counting” approach to estimate solute concentrations at an extraction well using particle-tracking flow models. The pathline counting approach is a unique method for predicting solute concentrations due to mixing. The pathline counting method using the semianalytical module RESSQC, contained within the WHPA model, was compared to MT3D, a numerical mass transport code, for a simple hypothetical problem. Results from the comparison indicate that results from the pathline counting approach were in reasonable agreement with the MT3D results. This approach was then applied to a field study, the Park City Bioremediation Project. The migration of a conservative tracer injected into three upgradient cells was simulated to estimate the concentration of solute at two pumping wells. The calculated concentrations compared favorably with measured field concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1745-6584.1994.tb00912.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Synergism of mosquitocidal toxicity between CytA and CrylVD proteins using inclusions produced from cloned genes of Bacillus thuringiensis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 13 (1994), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The toxicity to mosquito larvae of the parasporal body produced by Bacillus thuringiensis subsp. israelensis and the PG-14 isolate of B. thuringiensis subsp. morrisoni is at least 20-fold greater than any of the four mosquitocidal proteins of which It is composed (CytA, CrylVA, B, and D). This high toxicity is postulated to be due to synergistic interactions among parasporal proteins. However, this remains controversial because values reported for the specific toxicity of individual proteins, especially the CytA protein, vary widely owing to the methods used to purify and assay toxins against larvae. In an attempt to resolve questions of purity, specific toxicity, and synergism, individual genes encoding the CytA and CrylVD toxins were cloned and expressed in acrystalliferous B. thuringiensis subsp. israelensis cells using the shuttle vector pHT3101. CytA and CryIVD inclusions were purified and their toxicity was determined alone and when combined at different ratios using bio-assays against first instars of Aedes aegypti. The LC50 for the CytA inclusion was 60 ng ml−1, whereas the LC50 for the CryIVD was 85ng ml−1 In comparison, the LC50s for different combinations of CytA and CrylVD inclusions ranged from 12–15 ng ml−1, 4–5 times higher than the toxicity of either protein alone, demonstrating marked synergism between these two proteins. These results suggest that the high toxicity of the wild-type parasporal bodies of B. thuringiensis subspp. israelensis and morrisoni Is due to synergism among three or four of their major proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1994.tb00488.x
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  • 4
    Electronic Resource
    Electronic Resource
    Differential enhancement of Cry2A versus Cry11A yields in Bacillus thuringiensis by use of the cry3A STAB mRNA sequence (1999)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 181 (1999), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Previously we demonstrated that the yield of Cry3A (70 kDa) can be increased as much as 10-fold when cry3A including its upstream STAB-SD mRNA stabilizing sequence is expressed in Bacillus thuringiensis under the control of cyt1A promoters. To determine whether the cyt1A promoters/STAB-SD combination (cyt1AP/STAB) has broader applicability, we used it to synthesize two other Cry endotoxins in the 70-kDa mass range, Cry2A and Cry11A. Combination of cyt1AP/STAB with orfs 2 and 3 of the cry2A operon yielded about 4.4-fold the amount of Cry2A obtained with the wild-type cry2A operon. The yield of Cry11A obtained with a construct that contained the cyt1AP/STAB, cry11A and the 20-kDa protein gene was 1.3-fold the amount obtained with a construct similar to the wild-type operon. These results demonstrate that the cyt1AP/STAB combination can enhance synthesis of different Cry proteins significantly, but that the level of enhancement varies with the specific protein synthesized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb08862.x
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  • 5
    Electronic Resource
    Electronic Resource
    Parasporal body from Bacillus thuringiensis subsp. kenyae composed of a novel combination of inclusions and Cry proteins (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 134 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A new type of insecticidal parasporal body is reported from Bacillus thuringiensis subspecies kenyae (H4a4c) isolated from maize dust in Mexico. This parasporal body is spherical to oblong and composed of three inclusions, one spherical, one cuboidal, and one bipyramidal. Together, these contain four major proteins, three in the Cryl size class (138, 133, and 131 kDa), and one in the CryII size class (65 kDa). Monoclonal antibodies that recognize CryIB reacted with the 138-kDa protein, but those that recognize CryIA(a) or CryIA(a) and CryIA(b) did not react with any of the proteins. This isolate was more toxic (LC50= 96 ng cm−2 diet) to first instars of the diamond back moth, Plutella xylostella, than the HD1 isolate of B. thuringiensis subsp. kurstaki (LC50= 182 ng cm−2 diet).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07937.x
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  • 6
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    Unoccupied Aircraft Systems (UASs) Reveal the Morphological Changes at Stromboli Volcano (Italy) before, between, and after the 3 July and 28 August 2019 Paroxysmal Eruptions (2021)
    MDPI
    Details
    Publication Date: 2021-08-30
    Description: In July and August 2019, two paroxysmal eruptions dramatically changed the morphology of the crater terrace that hosts the active vents of Stromboli volcano (Italy). Here, we document these morphological changes, by using 2259 UAS-derived photographs from eight surveys and Structure-from-Motion (SfM) photogrammetric techniques, resulting in 3D point clouds, orthomosaics, and digital surface models (DSMs) with resolution ranging from 8.1 to 12.4 cm/pixel. We focus on the morphological evolution of volcanic features and volume changes in the crater terrace and the upper part of the underlying slope (Sciara del Fuoco). We identify both crater terrace and lava field variations, with vents shifting up to 47 m and the accumulation of tephra deposits. The maximum elevation changes related to the two paroxysmal eruptions (in between May and September 2019) range from +41.4 to −26.4 m at the lava field and N crater area, respectively. Throughout September 2018–June 2020, the total volume change in the surveyed area was +447,335 m3. Despite Stromboli being one of the best-studied volcanoes worldwide, the UAS-based photogrammetry products of this study provide unprecedented high spatiotemporal resolution observations of its entire summit area, in a period when volcanic activity made the classic field inspections and helicopter overflights too risky. Routinely applied UAS operations represent an effective and evolving tool for volcanic hazard assessment and to support decision-makers involved in volcanic surveillance and civil protection operations.
    Description: Published
    Description: 2870
    Description: 5V. Processi eruttivi e post-eruttivi
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 7
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    Chromatography, Vol. 2, Pages 567-579: Nanoparticle-Surface Interactions in Geometrical Separation Devices (2015)
    MDPI
    Details
    Publication Date: 2015-09-12
    Description: This study sought to demonstrate the impact of surface-target interactions in a device bearing geometrical features through evaluations of chemical modification to the surface and alteration of the charge state of both surface and targets. The movement of commercial fluorescent nanoparticles through a field of geometric features was monitored based on the output of the field measured at different points transverse to the direction of flow. Modification of the device surfaces using carboxylate, amine, phenyl, and fluorinated groups was considered. The functional group on the surface was found to significantly impact biased particle movement in the device with amine groups leading to the greatest differences. Other factors, such as flow rate, pH, and initial particle concentration, were also found to have a significant impact on particle concentrations across the feature field.
    Electronic ISSN: 2227-9075
    Topics: Chemistry and Pharmacology
    Published by MDPI
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  • 8
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    Chromatography, Vol. 2, Pages 567-579: Nanoparticle-Surface Interactions in Geometrical Separation Devices (2015)
    MDPI
    Details
    Publication Date: 2015
    Description: This study sought to demonstrate the impact of surface-target interactions in a device bearing geometrical features through evaluations of chemical modification to the surface and alteration of the charge state of both surface and targets. The movement of commercial fluorescent nanoparticles through a field of geometric features was monitored based on the output of the field measured at different points transverse to the direction of flow. Modification of the device surfaces using carboxylate, amine, phenyl, and fluorinated groups was considered. The functional group on the surface was found to significantly impact biased particle movement in the device with amine groups leading to the greatest differences. Other factors, such as flow rate, pH, and initial particle concentration, were also found to have a significant impact on particle concentrations across the feature field.
    Electronic ISSN: 2227-9075
    Topics: Chemistry and Pharmacology
    Published by MDPI
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  • 9
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    Suppression of C9orf72 RNA repeat-induced neurotoxicity by the ALS-associated RNA-binding protein Zfp106 (2017)
    eLife Sciences Publications
    Details
    Publication Date: 2017-01-10
    Description: Expanded GGGGCC repeats in the first intron of the C9orf72 gene represent the most common cause of familial amyotrophic lateral sclerosis (ALS), but the mechanisms underlying repeat-induced disease remain incompletely resolved. One proposed gain-of-function mechanism is that repeat-containing RNA forms aggregates that sequester RNA binding proteins, leading to altered RNA metabolism in motor neurons. Here, we identify the zinc finger protein Zfp106 as a specific GGGGCC RNA repeat-binding protein, and using affinity purification-mass spectrometry, we show that Zfp106 interacts with multiple other RNA binding proteins, including the ALS-associated factors TDP-43 and FUS. We also show that Zfp106 knockout mice develop severe motor neuron degeneration, which can be suppressed by transgenic restoration of Zfp106 specifically in motor neurons. Finally, we show that Zfp106 potently suppresses neurotoxicity in a Drosophila model of C9orf72 ALS. Thus, these studies identify Zfp106 as an RNA binding protein with important implications for ALS.
    Electronic ISSN: 2050-084X
    Topics: Biology , Medicine , Natural Sciences in General
    Published by eLife Sciences Publications
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  • 10
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    Chlamydia interfere with an interaction between the mannose-6-phosphate receptor and sorting nexins to counteract host restriction (2017)
    eLife Sciences Publications
    Details
    Publication Date: 2017-03-02
    Description: Chlamydia trachomatis is an obligate intracellular pathogen that resides in a membrane-bound compartment, the inclusion. The bacteria secrete a unique class of proteins, Incs, which insert into the inclusion membrane and modulate the host-bacterium interface. We previously reported that IncE binds specifically to the Sorting Nexin 5 Phox domain (SNX5-PX) and disrupts retromer trafficking. Here, we present the crystal structure of the SNX5-PX:IncE complex, showing IncE bound to a unique and highly conserved hydrophobic groove on SNX5. Mutagenesis of the SNX5-PX:IncE binding surface disrupts a previously unsuspected interaction between SNX5 and the cation-independent mannose-6-phosphate receptor (CI-MPR). Addition of IncE peptide inhibits the interaction of CI-MPR with SNX5. Finally, C. trachomatis infection interferes with the SNX5:CI-MPR interaction, suggesting that IncE and CI-MPR are dependent on the same binding surface on SNX5. Our results provide new insights into retromer assembly and underscore the power of using pathogens to discover disease-related cell biology.
    Electronic ISSN: 2050-084X
    Topics: Biology , Medicine , Natural Sciences in General
    Published by eLife Sciences Publications
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