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  • Column liquid chromatography
  • Immunocytochemistry
  • kinetics
  • wheat
  • 1970-1974  (12)
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Keywords
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Year
  • 1
    Electronic Resource
    Electronic Resource
    The role of solvent structure in ligand substitution and solvent exchange at some divalent transition-metal cations (1973)
    Springer
    Journal of solution chemistry 2 (1973), S. 217-238 
    Details
    ISSN: 1572-8927
    Keywords: Cation ; energies ; H. S. Frank ; M. Eigen ; R. G. Wilkins ; kinetics ; ligand substitution ; mechanism ; mixed solvents ; nonaqueous ; solvation ; solvent ; solvent structure ; solvent exchange ; transition metal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The role of the solvent in reactions involving ions is considered in relation to the structure of liquids. The rate constants and activation parameters for ligand substitutions at divalent transition metal cations in various solvents are compared with those for solvent exchanges. The differences are related to structural properties of the solvents, represented by their heats of evaporation and fluidities, and interpreted with the aid of a model developed from that of Frank and Wen. Water is not a typical solvent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00651974
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  • 2
    Electronic Resource
    Electronic Resource
    A correlation of gas solubilities and kinetics of hydrolysis in binary aqueous mixtures (1974)
    Springer
    Journal of solution chemistry 3 (1974), S. 881-887 
    Details
    ISSN: 1572-8927
    Keywords: Binary aqueous mixtures ; solubilities ; kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A treatment which predicts the solubilities of gases in solvent mixtures is examined in the context of the analysis of kinetic data for reactions in aqueous mixtures. The treatment is qualitatively successful to some extent in resolving the effects of changes in the initial state of the reacting substrate in the activation parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00647942
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  • 3
    Electronic Resource
    Electronic Resource
    Extracellular localization of cathepsin D in ossifying cartilage (1973)
    Springer
    Calcified tissue international 12 (1973), S. 313-321 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin D ; Extracellular ; Ossification ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé De la cathepsine D extracellulaire d'origine principalement ostéoblastique a été démontrée par immunohistochimie dans le cartilage en voide de calcification de cultures d'os des membres d'embryons de poulet. L'intérêt de ce fait pour l'ossification est discuté.
    Abstract: Zusammenfassung Es wurde mit einer immunohistochemischen Methode gezeigt, daß extrazelluläres Kathepsin D, welches hauptsächlich aus Osteoblasten gewonnen wurde, Knorpel von kultivierten Knochen von Kükenembryonen mineralisiert. Die Bedeutung dieser Feststellung für den Mechanismus der Knochenbildung wird diskutiert.
    Notes: Abstract Extracellular cathepsin D derived mainly from osteoblasts has been demonstrated immunohistochemically in ossifying cartilage of cultured embryonic chick limb bones. The relevance of this observation to the mechanism of ossification is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013744
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  • 4
    Electronic Resource
    Electronic Resource
    Kinetic studies on rifampicin (1972)
    Springer
    European journal of clinical pharmacology 5 (1972), S. 111-115 
    Details
    ISSN: 1432-1041
    Keywords: Rifampicin ; kinetics ; multicompartmental analysis ; simultaneous serum urine and bile assessment ; one week treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary To aid interpretation of the fall in the serum concentrations of rifampicin observed in the early phase of continuous treatment, the distribution of the antibiotic was assessed simultaneously in the serum, bile, and urine of patients treated for one week. Transfer constants between these compartments were calculated on the 1st and 7th day of treatment by multicompartmental kinetic analysis. — The results obtained suggest that the decrease in serum concentrations is due to enhanced biliary excretion; no significant change was observed in the amount of antibiotic excreted in the urine. The increased capacity of the liver to excrete rifampicin in bile might result from enzyme induction by the antibiotic in hepatocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00561755
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  • 5
    Electronic Resource
    Electronic Resource
    Purification and characterization of two allelic forms of l-glycerol 3-phosphate dehydrogenase from inbred strains of mice (1974)
    Springer
    Biochemical genetics 12 (1974), S. 69-79 
    Details
    ISSN: 1573-4927
    Keywords: l-glycerol 3-phosphate dehydrogenase, purified ; inbred mice ; kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract l-Glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8) was purified from the muscle of BALB/cJ and C57BL/6J mice. The half-lives of the enzyme at 50 C were 6 and 33 min, respectively, for the BALB/cJ and C57BL/6J strains. Enzyme preparations from the two strains of mice were compared with respect to the following properties and found to be essentially indistinguishable: K m values for dihydroxyacetone phosphate, NADH, l-α-glycerophosphate, and NAD+; maximum velocity; competitive inhibition by inorganic phosphate; pH optimum; energy of activation; electrophoretic mobility; molecular weight and subunit molecular weight. From these data, it is concluded that the kinetic properties of the purified enzyme are not the factors responsible for the differences in activity found in crude homogenates of mouse tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00487529
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  • 6
    Electronic Resource
    Electronic Resource
    Calculations of parabolic rate constants for metal oxidation (1974)
    Springer
    Oxidation of metals 8 (1974), S. 379-391 
    Details
    ISSN: 1573-4889
    Keywords: oxidation ; sulfidation ; kinetics ; parabolic rate law
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The conditions necessary to determine the oxidation kinetics of metals and alloys are discussed quantitatively, and a new method of calculating the rational rate constants was suggested. It was shown that the size and the shape of a metal sample has an effect on the kinetics of oxidation. Further, it was established that the values of the parabolic rate constants of corrosion, calculated from the empirical Pilling and Bedworth equation, contain a serious systematic error, if changes in the metallic core surface area during the oxidation process are neglected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00603388
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  • 7
    Electronic Resource
    Electronic Resource
    Kinetic characteristics of monoamine oxidase and serum cholinesterase in several related rat strains (1974)
    Springer
    Biochemical genetics 11 (1974), S. 309-317 
    Details
    ISSN: 1573-4927
    Keywords: genetics ; rat ; kinetics ; brain MAO ; serum ChE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study was performed in order to delineate differences in kinetic enzyme characteristics of brain monoamine oxidase (MAO) and plasma cholinesterase (ChE) derived from the Walker-Walker (Fawn Hooded, FH) rat and from its putative ancestors, the Wistar (W) and Long-Evans (LE). As compared with the enzyme isolated from the other two strains, brain MAO from FH has both a higher V max and increased reaction rate at lower substrate concentrations. It may thus be described as a “more efficient” enzyme. This study confirms previous work which shows that plasma ChE activity of females is higher than that of males. Fluoride ion is a noncompetitive inhibitor of the Wistar ChE, is a competitive inhibitor of the FH enzyme, and has no effect on the LE enzyme. Dibucaine is a competitive inhibitor in all cases except one: ChE derived from the FH female is uncompetitively inhibited. A comparison of the inhibitor constants shows that FH ChE is more resistant to Dibucaine than is that of W, and that LE is the most sensitive. FH cholinesterase is twice as resistant to the action of fluoride as is the Wistar enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00485998
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  • 8
    Electronic Resource
    Electronic Resource
    Luteinizing hormone —Releasing hormone (LH-RH) pathway of the rat hypothalamus revealed by the unlabeled antibody peroxidase-antiperoxidase method (1974)
    Springer
    Cell & tissue research 153 (1974), S. 211-217 
    Details
    ISSN: 1432-0878
    Keywords: Luteinizing hormone-releasing hormone ; Hypothalamus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Utilizing the unlabeled antibody enzyme method, we report the distribution of hypothalamic elements immunoreactive with antibodies to luteinizing hormone-releasing hormone (LH-RH) in the rat. Immunostained elements, resembling neural processes, were distributed along a pathway corresponding to the tuberoinfundibular tract which appeared to terminate near vascular elements in the external layer of the preand post-infundibular median eminence. No cell bodies stained specifically for LH-RH. Similar topographic arrangements were noted (in coronal and sagittal sections) in diestrous females, ovariectomized females and a hypophysectomized male. The same results were obtained with three different preparations of antisera to LH-RH. Our studies agree with those of other investigators using immunohistochemical techniques as well as with localization studies of LH-RH in the hypothalamus using bioassay and radioimmunoassay. Our results suggest that the unlabeled antibody enzyme technique will have unique value for identifying and tracing fiber systems related to specific functions within the hypothalamus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226609
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  • 9
    Electronic Resource
    Electronic Resource
    Localisation par immunocytologie de sécrétions apparentées aux hormones thyréotropes, gonadotropes et corticotropes dans l'hypophyse distale de l'Axolotl (Ambystoma mexicanum, Shaw) (1973)
    Springer
    Cell & tissue research 142 (1973), S. 539-548 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Axolotl ; Glycoprotein hormones ; Corticotrophs ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé Les anticorps anti-TSH (hormone thyréotrope) d'origine bovine, anti-LH β (hormone lutéinisante) d'origine ovine et anti β (1–24) corticotropine de synthèse ont été appliqués à l'hypophyse de l'Axolotl (Ambystoma mexicanum, Shaw) selon une technique d'immunofluorescence histologique indirecte. Les anticorps anti-TSH se fixent sur une population de cellules fusiformes localisées dans les régions caudale et ventrale de l'hypophyse distale. Les éléments révélés par les anticorps anti-LH β possèdent un noyau ovalaire entouré d'un cytoplasme intensément fluorescent et sont répartis dans l'ensemble du lobe hypophysaire distal. Les cellules fixant l'anticorps anti β (1–24) corticotropine sont disposées en bordure de l'éminence médiane et présentent un noyau ovalaire et un cytoplasme abondant. Il s'agirait respectivement des cellules à fonction thyréotrope, gonadotrope et corticotrope. L'examen des caractéristiques cytologiques et histochimiques de ces trois types cellulaires complète ces données immunologiques.
    Notes: Summary Antisera prepared respectively against TSH (thyreotropin) of bovine origin, against LH (luteotropin) of ovine origin and against synthetic β (1–24) corticotropin were applied to histological sections of Axolotl pituitary (Ambystoma mexicanum, Shaw) with the indirect immunofluorescence procedure. The anti-TSH antibodies from the first antiserum attach themselves to a population of fusiform cells situated in the caudal and ventral areas of the pars distalis. The cells revealed by the second antiserum show an oval nucleus surrounded by a strongly fluorescent cytoplasm. They are scattered throughout the whole pars distalis. The cells revealed by the third antiserum are arranged around the median eminence. Their nucleus is oval and their cytoplasm abundant. Presumably, these three cell types correspond respectively to the thyreotropic, gonadotropic and corticotropic cells as indicated by a parallel examination of their cytological and histochemical characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306714
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  • 10
    Electronic Resource
    Electronic Resource
    Les cellules corticotropes de l'hypophyse d'un Amphibien après interrénalectomie (1972)
    Springer
    Cell & tissue research 132 (1972), S. 333-364 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary gland ; Amphibians ; Corticotrophs ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'identification des cellules responsables de l'élaboration de la corticotropine (ACTH) a été envisagée chezRana esculenta. Les effects causés par l'interrénalectomie au niveau de l'hypophyse ont été étudiés parallèlement par immunofluorescence et au microscope électronique. Au microscope à fluorescence, les cellules détectées chez les animaux témoins avec un antisérum anti-synachten1 bordent en grand nombre les capillaires des zones médiorostrale et ventrale du lobe antérieur. Deux jours après interrénalectomie, le nombre de cellules fluorescentes décroît, douze jours après l'intervention, il ne subsiste pas d'éléments fluorescents dans cette zone. Au microscope électronique, les cellules corticotropes présentent de fines granulations d'environ 200 mμ de diamètre. Après interrénalectomie bilatérale, ces cellules sont fortement stimulées, elles sont sujettes à d'importantes modifications morphologiques; l'aspect morphologique des autres catégories de cellules antéhypophysaires, par contre, n'est pratiquement pas modifié. Douze jours après l'opération, la plupart de ces cellules sont dégranulées, l'ergastoplasme et l'appareil de Golgi sont bien développés. Ces observations suggèrent que les cellules péricapillaires de la moitié rostrale de lapars distalis sécrètent l'hormone corticotrope.
    Notes: Summary Identification of the cell types responsible for the production of adrenocorticotropin (ACTH) was performed inRana esculenta. The effects of interrenalectomy on the pituitary cells were studied as well by immunofluorescence, as by electron microscopy. In control animals, the ACTH cells studied by immunofluorescence are numerous around the blood vessels of the medio-rostral and medio-ventral part of the anterior lobe. Two days after interrenalectomy the number of fluorescent cells decreases. Twelve days after, the operation all the fluorescent cells disappeared. The fine structure of the corticotrophs is characterized by the presence of small secretory granules (200 mμ). After bilateral interrenalectomy this cell type is markedly stimulated; it displays striking morphological changes, while the morphology of the other pituitary cell types is not considerably modified. Twelve days after operation most of these cells are degranulated, the rough endoplasmic reticulum and the Golgi apparatus are well developed. These findings suggest that the pericapillary cells of the rostral half of thepars distalis produce the adrenocorticotrophic hormone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02450713
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