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  • Articles  (32)
  • Soybean  (32)
  • Springer  (32)
  • American Association for the Advancement of Science
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  • 1980-1984  (32)
  • 1960-1964
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  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (32)
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  • Articles  (32)
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  • Springer  (32)
  • American Association for the Advancement of Science
  • Annual Reviews
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  • 1
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    Plant and soil 54 (1980), S. 95-106 
    ISSN: 1573-5036
    Keywords: Acid soil ; Antibiotic resistance ; Competition ; Rhizobium ; Soybean ; Symbiotic nitrogen fixation ; Tropical Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficacy of inoculating soybean cvs. TGm294-4 and TGm80 in an acid soil (pH 4.6, water) was studied in a field trial in Nigeria.Rhizobium japonicum strains CB1809str r and 46spc r which had been selected for symbiotic competence in the acid soil were used as inocula. Other treatments included no inoculation, combined nitrogen (150 kg N ha−1) and lime (1 t ha−1). Plants grown in acid soil without inoculation and lime had very few nodules, were nitrogen deficient and grain yields were poor (0.3–0.4 t ha−1). Yield responses to combined nitrogen were generally small due to fertilizer loss through leaching. Inoculation greatly increased nodulation, shoot dry matter and shoot N%; grain yields were between 1.6 and 2.0 t ha−1. Lime improved all harvest measurements in the uninoculated and nitrogen treatments but not in the inoculated treatments. In a duplicate trial without lime on near-neutral soil, similar inoculation responses were obtained at 6 weeks. However, at final harvest there were no significant differences in grain yield between treatments due to late, effective nodulation by unidentified strains in the uninoculated treatments. Assays for strain identity confirmed the successful establishment of the inocula in both soils, with 100% of nodules from inoculated plants containing an introduced strain.
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  • 2
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    Plant and soil 54 (1980), S. 163-167 
    ISSN: 1573-5036
    Keywords: Phytoalexin ; Phytophthora megasperma ; Salinity ; Soybean ; Rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rhizosphere salinity decreased the capacity of soybean to accumulate a pterocarpanoid phytoalexin (glyceollin) in the stem in response toPhytophthora megasperma var.sojae. Rapid (48h) accumulation was depressed by NaCl, Na2SO4, CaCl2 and MgSO4 applications. Time-course accumulations was slowed by applications. Time-course accumulation was slowed by application of 0.131M NaCl. Glyceollin accumulation was also reduced in plants subjected to a period of high salinity stress (0.177M NaCl, 72 h) after a period of nonsalinized growth. Calcium chloride completely suppressed glyceollin accumulation in normally-resistant plants but no susceptibility to the fungus was observed.
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  • 3
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    Plant and soil 56 (1980), S. 27-32 
    ISSN: 1573-5036
    Keywords: Nitrate ; Nitrate reductase ; Nitrogen nutrition ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The seasonal and diurnal variations in nitrate reductase (NR) activity of field grown ‘Altona’ soybean, with and without applied nitrogen, were determined. The NR activity in the fortnightly collected leaf samples was, on the average, 20 percent higher throughout the season in N-treated plants, the highest being early in the season and declining gradually in the samples of subsequent dates. Diurnal variations were marked by increase in the NR activity from 7 a.m. to 7 p.m. and then declining gradually to a minimum at 7 a.m. the next morning.
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  • 4
    ISSN: 1573-5036
    Keywords: Alfalfa ; Alkaline uptake pattern ; Legumes ; Rock phosphate mobilization ; Soil acidification ; Soybean ; Symbiotic N fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean and alfalfa were grown on sand and soil to which P was added in the form of finely ground rock phosphates. When the legumes depended on NO3 as N source, more anionic than cationic nutrients were absorbed. This resulted in a pH increase in the growth medium and in very low availability of P added as rock phosphate. When, however, the legumes made use of symbiotically fixed N, more cationic than anionic nutrients were absorbed leading to an acidification of the growth medium and an ensuing mobilization and higher availability of the rock phosphates. Symbiotic N fixation which initiates the chain of reactions leading to an increased availability of rock phosphate-P is dependent on photosynthate supply and on the availability of phosphate. Therefore, in a separate experiment it was investigated whether a priming effect exerted by a small quantity of added easily soluble phosphate, could enhance the availability of rock phosphate-P to legumes. Results obtained indicated that easily soluble phosphate might indeed be effective in this respect.
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  • 5
    ISSN: 1573-5036
    Keywords: Calcium peroxide ; Cowpea ; Soybean ; Water table depth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of three water table (WT) depths (0, 15 and 40 cm) and calcium peroxide (Calper) on the growth and yield of cowpea (Vigna unguiculata, L.) and soybean (Glycine max) were investigated in field lysimeters for a sandy loam soil. Cowpea growth was the best at 40 cm WT depth. Leaf area, plant height, dry matter production, number of leaves and pods, grain yield and consumptive water use of cowpea increases with deeper (lower) WT depth. Application of calcium peroxide improved per cent emergence, leaf area, dry matter, number of leaves and pods, weight of 100 seeds, grain yield and water use in cowpea. The optimum WT depth for vegetative growth of soybean was 15 cm, although the highest grain yield was obtained at 40 cm WT depth. Number of pods, grain yield and water use efficiency of soybean increased with deeper water table depth. Application of calcium peroxide to soybean increased number of leaves and pods per plant, and grain yield for the 15 cm WT depth treatment.
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  • 6
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    Plant and soil 64 (1982), S. 325-330 
    ISSN: 1573-5036
    Keywords: Glomus spp. ; Glycine max ; Soybean ; VA mycorrhizae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Inoculation with vesicular-arbuscular (VA) mycorrhizal fungiGlomus fasciculatus, G. mosseae, G. etunicatus orAcaulospora scrobiculatus, increased plant dry weight and seed yields of pot-grown soybean plants in sterilized soil. Inoculation with a mixture ofG. fasciculatus, G. mosseae andG. etunicatus, orG. fasciculatus alone, increased seed yields and other agronomic traits of soybean plants grown in a no-tillage, rice-stubble field.
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  • 7
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    Plant and soil 65 (1982), S. 73-77 
    ISSN: 1573-5036
    Keywords: Seed emergence ; Seed environment ; Soil crusting ; Soybean ; Varietal differences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The fluctuations of ODR, moisture content in the crust and the soil beneath the crust and crust strength during emergence period have been presented. The limiting factors of the seed environment under crusted soil conditions have been described and identified. In the early part of the emergence period ODR and in the later part crust strength were the limiting factors. The emergence characteristics of the 12 soybean varieties of varying seed sizes were analysed in detail. The small seeded varieties (Type-1 and Type-49) were less susceptible to the effects of increasing crust strength and maintained distinct emergence advantage over the large seeded varieties. Since the large seeded varieties confronted the hard crust barriers for a longer period of time, their seedling mortality was more than 50 per cent as compared to around 30 per cent in case of small seeded ones.
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  • 8
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    Plant and soil 66 (1982), S. 391-396 
    ISSN: 1573-5036
    Keywords: Soybean ; Hydroponics ; Nitrogen source ; Plant nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean plants were grown in nutrient culture solutions containing 150 ppm of N either as an equal concentration of NH4 + or NO3 −, or all NO3 −. At the R2 stage of growth for some plants, the N form was changed to either all NO3 − or all NH4 +, but at the same total N concentration as before. Highest seed yield was obtained with all NO3 − over the entire growth period, the poorest when the N form was switched from an equal ratio of NH4 + and NO3 − to all NH4 + at the R2 stage. Kjeldahl N concentrations in the plant leaves and seed were highest when NH4 + was part or all of the N source in the nutrient solution. These results may partially explain why the literature is inconsistent on the effect of added fertilizer N on soybean seed yield, and may pose a problem in using leaf Kjeldahl N concentration to determine plant N sufficiency.
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  • 9
    ISSN: 1573-5036
    Keywords: Glomus fasciculatum ; glycine max ; Mycorrhiza ; Phosphorus ; Soybean ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean plants colonized by the vesicular-arbuscular mycorrhizal (VAM) fungusGlomus fasciculatum were grown in pot cultures utilizing a composite greenhouse rooting medium. Development of fungal mycelia inside and outside the host root and total fungal biomass were determined from assays of fungal chitin. Growth and phosphorus uptake by VAM plants and uncolonized controls were compared. Mycotrophic growth in VAM plants occurred during the final six weeks of the 19-week growth period, when the concentration of available soil P fell below 10 μg P/g soil. Growth enhancement was most pronounced in the reproductive organs. The data suggest a relationship between the initiation of the reproductive phase in the host and the cessation of growth in the endophyte. Source-sink relationships and P availability appear to be factors influencing interactions between the symbionts.
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  • 10
    ISSN: 1573-5036
    Keywords: Acid soils ; Nitrogen fertilizer ; Rhizobium ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean cultivar MTD10 cultivated on a moderate acid paddy soil of the Mekong Delta responded well to a multistrain inoculation and to chemical nitrogen fertilizer. However, the efficiency of chemical nitrogen uptake by the uninoculated plants decreased with increasing nitrogen application rate. Inoculation improved all examined agronomic characteristics of plants. Inoculated plants produced grain yield 10 times greater than the uninoculated controls and 2.5 times greater than the uninoculated plants receiving 80 kg/ha of chemical nitrogen. Inoculation was superior to chemical nitrogen fertilization in all parameters of importance to market-oriented farmers.
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  • 11
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    Plant and soil 74 (1983), S. 273-280 
    ISSN: 1573-5036
    Keywords: Germination ; Glycine max ; Mulches ; Sandy loam ; Soil moisture ; Soil temperature ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Experiments were conducted in a sandy loam soil, under both laboratory and field conditions. In the laboratory, germination was studied at 5, 10, 15 and 20 per cent soil moisture and in the aerated distilled water at 12±0.5, 20±0.5, 28±0.5, 36±0.5 and 43±0.5°C soil temperatures. Results showed that the optimum soil temperature for soybean germination was 28°C and the optimum soil moisture was between 10–15 per cent in this soil type. At 28°C and 5 per cent soil moisture, the time required for 75 per cent germination was 71 hours which reduced to 47 and 35 hours at 10 and 15 per cent soil moistures, respectively. In the field, soil temperature was regulated using mulches,viz polythene (Transparant) and three retes of straw (5, 10 and 15 t/ha, referred as S1, S2 and S3, respectively). Two seed rates,viz normal (80 kg/ha) and double (160 kg/ha) were used. Soil temperature at 5 cm depth ranged from 26·6 to 44·6°C under polythene, 24·2 to 32·8°C in S1, 24.3 to 30.4°C in S2, 24.5 to 29.0°C in S3 and 23.0 to 38.4°C in control during the daylight hours. Water uptake by seed was highest under polythene and lowest in control but there was no germination in the polythene-mulched plot due to fungus, attact at 59 hours after sowing. A soil temperature regime of 24.2 to 32.8°C (created in S1) was optimum for germination. In all the mulched plots, effective germination in the double seed rate was never more than in the corresponding normal seed rate. Also, time taken for 75 per cent germination was minimum (71 hours) in S1 with normal seed rate and it took about 16 hours more with double seed rate.
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  • 12
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    Plant and soil 66 (1982), S. 439-442 
    ISSN: 1573-5036
    Keywords: N2-fixation ; Nodulation ; Rhizobium ; Soybean ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of mineral nitrogen on establishment and activity of symbioses between soybean and several strains ofRhizobium japonicum and on the establishment of nodules ofR. japonicum isolated from nodules of field crops is studied. All strains were highly susceptible to the effects of 200 ppm NO3−N on the establishment of symbiosis; 50 ppm NO3−N had little effect. Response of symbioses establishhed in the absence of mineral N to short term exposure to nitrate or ammonium varied significantly between strains. Nodule isolates from soybean crops growing in nitrifying soil were no less susceptible to the inhibitory effects of mineral N on nodule formation than a laboratory culture of the commercial inoculant strain.
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  • 13
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    Plant and soil 68 (1982), S. 171-181 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Cowpea ; Fertilizer-N ; Glycine max ; Nitrogen-15 ; Nodule ; Soybean ; Vigna unguiculata ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a Nigerian soil depleted in available N, fertilizer-N enriched in15N was applied at 25 and 100 kg N/ha to crops of four cowpea and two soybean cultivars. Soil-N availability was estimated with three non-fixing crops, non-nodulating soybean, maize and celosia. With sequential harvests we examined the development of the fixing crops, as well as their nodulation profiles and acetylene reduction activities, and the patterns obtained were correlated with uptake of mineral-N. At low levels of mineral-N, excellent nodulation (up to 580 mg dry weight/plant) and very high acetylene reduction activities (up to 208 μmoles/plant/h) were recorded. Although fertilizer-N utilizations were low, 12% and 28% at 25 and 100 kg N/ha respectively, the lower application had a transient adverse effect on nodule development and the higher application had a long-term adverse effect on nodule formation, nodule development and acetylene reduction activity. Cowpea nodule mass reached maximum levels at early pod-fill except at 100 kg N/ha at which it continued to increase into late podfill. In contrast, soybean nodulation did not peak at any level of N but increased into late pod-fill.
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  • 14
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    Plant and soil 68 (1982), S. 321-329 
    ISSN: 1573-5036
    Keywords: Alginate ; A value ; Endomycorrhiza ; Glomus mosseae ; Inoculant ; Nitrogen fixation ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This field study was undertaken to determine the effect of inoculation withGlomus mosseae on N2 fixation and P uptake by soybean. The inoculation withGlomus mosseae was achieved using a new type of inoculant, alginate-entrapped (AE) endomycorrhizal fungus. N2 fixation was assessed using the A value method. In P-fertilized plots, inoculation with AEGlomus mosseae increased the harvest index based on dry weight (+20%) and N content of seeds (+17%), the A value (+31%) and %N derived from fixation (+75%). Inoculation with AEGlomus mosseae decreased the coefficient of variation for the A value and for the dry weights of the different plant parts.
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  • 15
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    Plant and soil 75 (1983), S. 265-268 
    ISSN: 1573-5036
    Keywords: Agglutination ; Lectins ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Conditions leading to agglutination ofRhizobium japonicum 3I1b110 with soybean seed lectin were examined. Ability of cells to be agglutinated was transient and was optimal for cultures grown for 4–5 days on yeast extract mannitol plates. Similar lectin-binding results were obtained with cells from the same cultures using fluorescence microscopy with fluorescein isothiocyanate-labelled lectin. These results revise the previous model for soybean lectin-R. japonicum interactions, since it was based on the inability of soybean lectin to agglutinate these bacteria.
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  • 16
    ISSN: 1573-5036
    Keywords: δ15N variations ; N2 fixation ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This paper reports a field study for the assessment of the variation in15N natural abundance method to estimate nitrogen fixation. For two successive years (1979 and 1980) at the same site, distribution of the variable δ15N in populations of nodulating and non-nodulating soybeans (Glycine max) has been studied. In 1980, the population structure was studied in order to detect a neighbour effect which could explain bimodal trend observed in the distribution. There was no evidence for such an effect. A sampling procedure is proposed: with 15 to 30 nodulating plants chosen randomly, a confidence interval of ±0.5δ can be obtained, while less than 10 non-nodulating plants are sufficient to achieve a very good precision. Some other aspects are studied: effect of pooling plants which considerably reduce the experimental work; the use of a non leguminous reference plant is possible. All our results show that fixing capacity introduces major variability in the δ15N measured in the plants, while the absence of fixation leads to a homogenous population, so the percentage of fixed N can be evaluated with a rather good precision. The higher the reference value, the better the accuracy of this evaluation, for a given precision of a δ15N measurement.
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  • 17
    ISSN: 1573-5036
    Keywords: Endomycorrhizae ; Glomus fasciculatum ; Glycine max ; Soybean ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Development of a vesicular-arbuscular mycorrhizal (VAM) fungus in association with soybean was determined in a greenhouse soil mix by chitin assay. Samples were sieved to eliminate hexosamine-containing contaminants. This preparation reduced the interference caused by extraneous soil substances and permitted quantitative measurement of extraradical VAM fungal mycelium in the soil mix by colorimetric assay. Recovery of added chitin, used as an internal standard, was greater in the soil mix than in an inert medium indicating that some hexosamine was stabalized from chemical degradation by other soil components.
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  • 18
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    Plant and soil 68 (1982), S. 183-192 
    ISSN: 1573-5036
    Keywords: ‘AN’-value ; Cowpea ; Difference method ; Fertilizer-N ; Glycine max ; Nitrogen-15 ; N-balance ; N2-fixation ; Soybean ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amounts of N fixed in the field in Nigeria by four cowpea and two soybean cultivars were determined at different levels of available N by the difference and the ‘AN’-value methods using non-nodulating soybean, maize and celosia as non-fixing controls. No significant differences were found between the two methods or with different controls. Grain removal and return of residues to the soil were measured and net soil-N changes calculated. N-benefits accrued from two of the cowpea cultivars when grown at low mineral-N levels, but with higher mineral-N availability, no cultivar contributed N because of inhibition of nodulation and lowered N2-fixation. Although the soybeans fixed more N than the cowpeas they caused greater net N-depletions because they sequestered a greater fraction of N in the grain.
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  • 19
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    Plant and soil 70 (1983), S. 133-140 
    ISSN: 1573-5036
    Keywords: Glycine max L. Merr ; Nitrogen ; Soybean ; Sulphur deficiency ; Vertisols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Some soil and plant test methods were evaluated for predicting response of soybean crop (Glycine max (L.) Merr.) to S application in vertisols. Morgan's reagent, 500 ppm P containing Ca(H2PO4)2.H2O and KH2PO4 solutions, 0.5N NH4OAc+0.25N HOAc and 0.15% CaCl2 were found to be suitable extractants for measuring available soil S. The critical limits of extractable S were 9.0 ppm by Morgan's reagent, 10.0 ppm by phosphate solutions, 8.0 ppm by 0.5N NH4OAc +0.25N HOAc and 14.0 ppm by 0.15% CaCl2. Morgan's reagent was regarded as superior to other soil test methods in view of its high relationship with S uptake by plants, A values and relative yield. Critical S concentration in soybean plants varied with age. It was 0.15% and 0.185% for 36 and 60 days old plants, respectively. The critical N/S ratio on the other hand appeared to be constant at about 16.5 during vegetative growth period. Constancy of critical N/S ratio in plants was attributed to the near constancy of N/S ratio in plant proteins. There was highly significant relationship between response of soybean to S and to N, supporting the conclusion of some earlier workers that any soil showing large responses to N may not be supplying adequate S from the mineralization of soil organic matter.
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  • 20
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Glycine max L. ; Nitrogen fixation ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A system for employing open-ended root chambers to measurein situ acetylene reduction rates under field conditions is described. Gas mixtures containing about 2 mbar acetylene were continuously flowed through the chambers providing a continuous record of acetylene reduction. These chambers have been used to measure acetylene reduction rates of soybeans during three growing seasons. The system has proved to be reliable with a high degree of precision. The large amount of plant-to-plant variability observed in N2 fixation research has been confirmed by the data collected with this system. However, such variability in physiological studies can be reduced by using a non-destructive system to compare the response of an individual plant with its rates before treatment.
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  • 21
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    Plant and soil 70 (1983), S. 317-326 
    ISSN: 1573-5036
    Keywords: Glycine max (L.) Merr. ; Iron ; Manganese ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The uptake and distribution of iron and manganese were studied in a manganese-sensitive soybean cultivar (‘Bragg’) grown over a range of supply levels of these nutrients in solution culture. At high (90 and 275 μM) manganese levels, increasing the iron concentration in solution from 2 to 100 μM partially overcame the effects of manganese toxicity. Interactions between manganese and iron occurred for dry matter yields, rate of Mn absorption by the roots, and the proportions of manganese and iron transported to the tops. No interaction was observed for the rate of root absorption of iron. The percentage distribution of manganese in the plant top increased with increasing iron, despite a reduced rate of Mn uptake. On the other hand, iron uptake was independent of solution Mn concentration and increased with increasing solution Fe. Also more iron was retained in the roots at high Mn and/or Fe levels in solution. Concentrations of manganese and iron in roots, stems and individual leaves were affected independently by the manganese and iron supplyi.e. without any interaction occurring between the two elements. In general, the concentration in a plant part was related directly to the solution concentration. Symptoms resembling iron deficiency correlated poorly with leaf Fe concentrations whereas high levels of manganese were found in leaves displaying Mn toxicity symptoms.
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  • 22
    ISSN: 1573-5036
    Keywords: Beta distribution ; Cotton ; Pea ; Peanut ; Pearson distribution ; Penetrometer ; Safflower ; Soybean ; Sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary It is shown that root maximum growth pressures, root buckling stresses, and soil penetration strengths and all non-normally distributed with positive skew. It is found that a Beta distribution, with assigned end points, provides an extremely good fit in each case. It is recommended that this Beta distribution is used in the development of mathematical models for root growth in soil.
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  • 23
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Glycine max L. Merr. ; N-difference method ; N2 fixation ; 15N methodology ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Isotopic as well as non-isotopic methods were used to assess symbiotic nitrogen fixation within eight soybean [Glycine max (L.) Merr.] cultivars grown at 20 and 100 kg N/ha levels of nitrogen fertilizer under field conditions. The15N methodology revealed large differences between soybean cultivars in their abilities to support nitrogen fixation. In almost all cases, the application of 100 kg N/ha resulted in lower N2 fixed in soybean than at 20 kg N/ha in the first year of the study. However, N2 fixed in one cultivar, Dunadja, was not significantly affected by the higher rate of N fertilizer application. These results were confirmed by measurements of acetylene reduction activity, nodule dry weight and N2 fixed as measured by the difference method. Further proof of differences in N2 fixed within soybean cultivars and the ability of Dunadja to fix similar amounts of N2 at 20 and 100 kg N/ha was obtained during a second year experiment. Dunadja yield was affected by N fertilizer and produced larger yield at 100 kg N/ha than at 20 kg N/ha. This type of cultivar could be particularly useful in situations where soil N levels are high or where there is need to apply high amounts of N fertilizer. The present study reveals the great variability between legume germplasms in the ability to fix N2 at different inorganic N levels, and also the potential that exists in breeding for nitrogen fixation associative traits. The15N methodology offers a unique tool to evaluate germplasms directly in the field for their N2 fixation abilities at different N fertilizer levels.
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  • 24
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    Plant and soil 56 (1980), S. 217-227 
    ISSN: 1573-5036
    Keywords: Antibiotic resistant mutants ; Immunodiffusion ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Antibiotic resistant mutants 8-0 StrR, 110 TetR and 138 KanR derived from wild typeRhizobium japonicum strains were inoculated into silt loam soil to cell concentrations greater than 2×108/g of soil. Population changes were monitored using antibiotic media and strain identification was done using immunodiffusion assay on microcores of soil. Immunodiffusion bands formed by the mutant strains with homologous antisera essentially duplicated bands formed by the parent strain. Strains 110 TetR and 8-0 StrR had cross reacting antigens whereas antigens of strain 138 KanR reacted only with the homologous antiserum. Populations ofR. japonicum strains introduced into sterile soil increased over a period of four weeks under both single and mixed culture inoculations. All populations decreased by the end of six weeks and thereafter remained constant. When theseR. japonicum strains were introduced into non-sterile soil, the population did not increase over the initial population added. Population decreased gradually for two weeks and then maintained thereafter. It was possible to recover very low populations of antibiotic resistantR. japonicum strains from both sterile and unsterile soils using media containing specific antibiotics. Detection ofR. japonicum strains by immunodiffusion was accomplished only when the population was ≥109 cells/g of soil. The method using antibiotic resistant mutants permitted an evaluation of the interactions of variousR. japonicum strains in soil with respect to their survival and multiplication.
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  • 25
    ISSN: 1573-5036
    Keywords: K supply ; K Mg uptake ; Mn nutrition ; Soybean ; Short-term ; Water culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean (Glycine max (L) Merr. cv. Bragg) seedlings were grown in nutrient solutions to evaluate the response to manganese nutrition as affected by potassium supply. In solutions containing 275 μM manganese, increasing the solution concentration of potassium from 1 mM to 10 mM alleviated symptoms of manganese toxicity, decreased manganese concentrations in the leaves and increased dry matter yields of the plants. The reduction in manganese toxicity was brought about by a reduced rate of root absorption of manganese at high potassium supply levels. Increasing the supply of either potassium or manganese decreased the leaf concentration of magnesium although there were no apparent symptoms of magnesium deficiency in any treatment. The reduced concentration of magnesium in the leaves was due to effects of potassium and manganese on the rate of root absorption of magnesium. Under manganese deficiency conditions, growth was reduced and manganese concentrations in plant parts were very low; there was no effect of potassium supply when manganese was absent from the nutrient solution.
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  • 26
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    Plant and soil 62 (1981), S. 97-105 
    ISSN: 1573-5036
    Keywords: Seedling emergence ; Soil crusting ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Attempts were made to work out the effect of crust impedance to seedling emergence under natural field conditions by photographing a series of positions taken by the emerging hypocotyls. Being epigeal in germination, the seedling had to work through a large volume of soil to pull the cotyledons out of the ground. Since the hypocotyl was brittle, it often collapsed at the stress point between the emerging hypocotyl and the buried cotyledons. A number of seedlings subjected to severe impedance stresses often broke off at the hypocotyl hook when the soil surface ruptured. The emergence force of the varieties tested was closely related to their seed weight. The large seeded variety ‘Bragg’ exerted the greatest emergence force (1.86×105 dynes) and the small seeded variety ‘Type-1’ exerted the least (1.24×105 dynes). The variety ‘UPSS-38’ was intermediate both in hundred seed weight and the force that it exerted (1.49×105 dynes). The time taken to develop the steady maximum force was 78, 67 and 60 hours for Bragg, UPSS-38 and Type-1 respectively.
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  • 27
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    Plant and soil 61 (1981), S. 71-80 
    ISSN: 1573-5036
    Keywords: Ecology ; Fluorescent antibody ; Immunofluorescence ; Rhizobium japonicum ; Rhizosphere ; Root surfaces ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Populations of nativeRhizobium japonicum 123 in the rhizospheres of field and pot grown plants as determined by immunofluorescence were calculated on the basis of root surface area. The density ofR. japonicum 123 on the root fluctuated between a few hundred to over a thousand per square centimeter of root surface. As root volume expanded rapidly, the Rhizobium density fell to less than one hundred per unit area. There was no appreciable effect due to different plant, nitrogen amendment, or addition of another strain ofR. japonicum, on the surface density of the nativeR. japonicum population on roots. Nor did the native population influence the added strain. Direct examination of root surface segments revealed that naturalized rhizobia existed sparsely on root surfaces in the form of short rods. They were observed to be attached sideways or in a polar manner on root hairs, epidermal cells, and at junctions of tap and lateral roots. There was no evidence of specific stimulation of the homologous Rhizobium by the host plant as a prelude to nodulation.
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  • 28
    ISSN: 1573-5036
    Keywords: Glycine max ; Isotope dilution technique ; Nitrogen-15 ; Nitrogen-fixation ; Organic matter ; Substrate-labelling ; Saccharum sp. ; Soybean ; Sugarcane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two varieties of sugarcane, and nodulated and non-nodulated soybean isolines, were planted in a soil previously mixed with15N-labelled plant material. 45 days was allowed to elapse before planting, to permit initiation of organic matter mineralization. Plants were grown for 60 days, then harvested, dried, weighed and analysed for total N. Analysis of soil samples pre-incubated in the laboratory was carried out to evaluate ammonium and nitrate from added organic matter. Dry weights of the soybean isolines were similar, but total N was higher for the nodulated line. Both sugarcane varieties showed similar weight and total N. Nitrogen derived from applied organic matter (NdfOM) was higher in non-nodulated soybean than in all other plants. Although there is the possibility of different15N availabilities between species, nitrogen derived from fixation (Nfix) was calculated based on the15N enrichment of the non-nodulating soybean. Nfix was 72% for nodulating soybean and ranged from 19 to 39% for different parts of sugarcane plants, despite high levels of available-N. Nitrogen derived from soil was calculated by difference. NdfOM was lower in roots than in upper parts (leaves+stalks) of plants. Use of15N labelled organic matter seems a useful approach to the longer term measurement of N2-fixation.
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  • 29
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    Plant and soil 82 (1984), S. 369-375 
    ISSN: 1573-5036
    Keywords: Glycine max. (L.) ; Merr. N2-fixation ; 15N methodology ; N yield ; Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Small differences in N2 fixation by nodulated soybeans (Glycine max. (L.) Merr.), inoculated with various strains ofRhizobium japonicum, were assessed in field experiments using15N methodology, and compared with yields of plant dry matter and total N. Percentage of plant-N derived from atmospheric N2 and from fertilizer, and values of %15N atom excess had lower coefficients of variation than did total N and dry matter yield. Nevertheless the precision of estimates of kg N/ha fixed were sufficient to differentiate only the extremes of the range of strains tested, and there were discrepancies between ranking of strains based on % N derived from fertilizer and on total N yield.
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  • 30
    ISSN: 1573-5036
    Keywords: Breeding ; Glycine max ; Nodulation ; Rhizobium spp. ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Most soybean varieties fail to nodulate effectively in tropical soils unless inoculated with a competitive strain ofRhizobium japonicum. Developing countries in the tropics, with few exceptions, lack inoculant industries to produce and distribute viable inoculants to small farmers and extension programs to teach them to use inoculant. Several soybean genotypes have been identified that nodulate effectively with many strains of the “cowpea” inoculation group which is ubiquitous in tropical soils of Africa. Soybean genotypes that nodulate and grow well without inoculant application are called “promiscuous”. Methodologies for incorporation of the promiscuity character into high-yielding backgrounds are discussed.
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  • 31
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    Plant and soil 80 (1984), S. 297-300 
    ISSN: 1573-5036
    Keywords: Nodulation ; Non-nodulating soybean ; Rhizobitoxine ; Rhizobium ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A significant increase in nodulation of “non-nodulating” (rj1 rj1) soybeans was obtained by inoculating with very high numbers (approx. 1011 cells/pot) of certain rhizobia when compared with inoculation at a moderate dose (approx. 109 cells/pot). Nodulating ability of rhizobial strains was not correlated with their ability to produce a detectable level of chlorosis-inducing toxin in culture.
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  • 32
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    Plant and soil 81 (1984), S. 309-310 
    ISSN: 1573-5036
    Keywords: Rhizobium japonicum ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Relative efficiency of Hup+ and Hup− R. japonicum strains with Pusa 16 cultivar of soybean was studied. Inoculation with the Hup+ strain (A 1014) reduced the protein content in grain as compared to uninoculated control.
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