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  • Articles  (1,669)
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  • Articles  (1,669)
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  • 1
    ISSN: 1432-0827
    Keywords: Osteoporosis ; Immobilization ; Rats ; Vitamin D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The therapeutic effects of vitamin D analogs, 1,24(R)-dihydroxycholecalciferol [1,24(R)(OH)2D3], 1,24(S)-dihydroxycholecalciferol [1,24(S)(OH)2D3], and 1,25-dihydroxycholecalciferol [1,25(OH)2D3] on immobilization osteoporosis were studied in rats. The right hind limb was immobilized through application of a plaster cast following the section of the sciatic nerve. The left hind limb was intact. Vitamin D analogs were orally administered for 6 weeks at dose levels of 0.02 and 0.10µg/kg/day, respectively. The mean lengths of the immobilized femurs were not significantly different from those of the intact femurs in all the experimental groups. In the immobilized femur of animals treated with 1,24(R)(OH)2D3, 0.10µg/kg, dry and ash weights were heavier and calcium and phosphorus contents greater than those in the nontreated group. Furthermore, the amount of calcified bone mass and the cortical thickness of the femurs of the immobilized limb in 1,24(R)(OH)2D3-treated animals were greater than those in the nontreated animals. Treatment with 1,25(OH)2D3 at 0.10µg/kg caused an increase of the bone mass in both immobilized and intact femurs when compared with those of the control group. It was concluded that the administration of 1,24(R)(OH)2D3 diminished the effect of immobilization in the development of osteoporosis without any side effects.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 34 (1982), S. 547-552 
    ISSN: 1432-0827
    Keywords: Rats ; Osteoporosis ; Anorganic ; Femur ; Castrate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The study describes the SEM appearances of endosteal and periosteal surfaces of anorganic femoral diaphyses from 16-month-old normal and castrate male rats. Different types of surfaces could be recognized in both groups. Percentage areas occupied by each surface type were analyzed with a Ladd Data Analyzing Digitizer. Endosteal surfaces were composed of significantly more (P〈0.05) incompletely mineralized, forming surface and significantly less (P〈0.05) completely mineralized, resting surface in castrates than in controls. Both endosteal and periosteal surfaces from experimental bone demonstrated significantly more (P〈0.05) osteoblast lucunae than did control surfaces, and vascular canal entrances were significantly wider (P〈0.001) on castrate endosteal surfaces than on control endosteal surfaces. There was a greater proportion of small nodule forming surface/large nodule forming surface in castrate endosteal bone than in control, and a greater proportion prolonged resting surface/fibrous resting surface in control periosteal bone than in castrate. The results indicate that, when viewed in the SEM, anorganic endosteal and periosteal bone surfaces from femoral diaphyses of old castrate male rats demonstrate appearances characteristic of changes in bone turnover that occur with osteoporosis.
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  • 3
    ISSN: 1432-0827
    Keywords: Boen ; Metabolism ; Rats ; Strontium ; Tooth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'on a procédé à une étude approfondie sur l'incorporation et l'enlèvement du strontium des os et des dents de rats dans les cas de régimes alimentaires à forte et faible teneur en strontium. Dans le cas d'un régime par ailleurs en tous points satisfaisant, l'adjonction d'une quantité de strontium de masse égale à celle du calcium provoque un phénomene débilitant très severe chez le jeune rat en pleine croissance, et peut même conduire à la mort du sujet. Les effets pathogéniques du strontium ont été découverts par des méthodes utilisées seulement en recherche dans les tissus durs. L'excédent de cellules osseuses, formées à l'origine du fait de la présence de grandes quantités de strontium dans l'alimentation, se résorbe si l'on passe à un regime à faible teneur en strontium. Il apparait qu'il y a deux types d'élimination dustrontium de l'organisme: a) par excrétion, et b) par assimilation dans les tissus durs. L'on n'a plus à démontrer que le strontium s'intègre par transfert dans les incisives. L'on a également observé, chez les sujets expérimentaux aussi bien que chez ceux du groupe de vérification, que des différences dans le taux de sodium et de potassium apparaissent dans les semi-mandibules.
    Abstract: Zusammenfassung Die Einlagerung sowie die Resorption von Strontium wurde an Rattenknochen und-zähnen mittels Zugabe von niedrigen und hohen Strontiumdosen zu der Diät untersucht. Wird bei einer sonst befriedigenden Diät Calcium durch äquimolare Strontiummengen ersetzt, so entsteht eine Schwächung bei jungen wachsenden Ratten, die bis zum Tode führen kann. Die pathologischen, durch Strontium entstandenen Veränderungen wurden nur mit denjenigen Methoden verfolgt, die zur Untersuchung der harten Gewebe angewendet werden. Das Übermaß an osteoidem Gewebe, welches ursprünglich bei Zugabe großer Strontiummengen zu der Nahrung entsteht, wird in einer darauffolgenden strontiumarmen Diätperiode resorbiert. Die Resorption des Strontiums scheint an zwei physiologische Prozesse gebunden zu sein: a) Ausscheidung aus dem Organismus; b) Eingliederung in die harten Gewebe. Der Strontiumtransport in den Schneidezähnen ist bewiesen worden. Es wurden auch Unterschiede im Natrium- und Kaliumgehalt des halben Unterkiefers bei Experiment- und Kontrollgruppen beobachtet.
    Notes: Abstract The incorporation and removal of strontium from the bones and teeth of rats under conditions of low and high dietary levels of strontium were investigated. In an otherwise satisfactory diet, an amount of strontium equimolar to that of calcium seriously debilitates the young growing rat and may culminate in death. The pathology due to strontium was found by the methods used only in the hard tissues. The excess osteoid formed originally in the presence of large amounts of dietary strontium is removed during a subsequent period of feeding on a low strontium regimen. Strontium removal from participation in physiologic processes appears to be of two types, (a) excretion from the body, and (b) incarceration within the hard tissues. The translocation of strontium to the incisor teeth has been demonstrated. Differences of sodium and potassium contents of the hemi-mandibulae of the experimental and control groups were also observed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 31 (1980), S. 45-47 
    ISSN: 1432-0827
    Keywords: Dermestid beetles ; Cleaning bones ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Various parts of the skeleton of normal and osteoporotic rats were compared with respect to their dry weight, ash weight, and calcium content when the bones were cleaned byDermestes maculatus beetles or manually. Both techniques gave similar results. This was also true when whole body calcium measured by neutron activation and total skeletal calcium from bones cleaned by the beetles were compared. Thus dermestid beetles are useful as a technique to clean bones, especially for the parts of the skeleton which are difficult to dissect by hand.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 32 (1980), S. 77-82 
    ISSN: 1432-0827
    Keywords: Osteoporosis ; Castration ; Density ; Femur ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Forty young (23-day-old) and thirty old (1-year-old) male rats were castrated and sacrificed with controls at intervals up to 18 months of age. No differences were observed between femurs or mandibles of rats castrated at 23 days and those of controls. Year-old castrate rats developed femoral osteoporosis after 2 months, which became more pronounced 4 months after castration. This was characterized by reductions in femoral density, dry weight, dry weight per unit length, and ash weight, and by the appearance of resorption cavities in diaphyseal walls and a sparsity of trabeculae in metaphyses and epiphyses of castrate femurs. These results indicate that the year-old castrate male rat may be a valuable experimental model for studies of the treatment of osteoporosis.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 34 (1982), S. 510-514 
    ISSN: 1432-0827
    Keywords: Rats ; Osteopenia of oophorectomy ; 1,25-Dihydroxyvitamin D3 ; Parathyroid hormone ; Osteoporosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effect of ovarian insufficiency on calcium metabolism has been thought to involve an increased bone resorptive effect of parathyroid hormone and possible impaired synthesis of 1,25-dihydroxyvitamin D3. In the present study a rat model allowing for controlled serum levels of parathyroid hormone and 1,25-dihydroxyvitamin D3 was used. Oophorectomy in this species is associated with increased serum levels of 1,25-dihydroxyvitamin D3 and decreased bone mass. Although thyroparathyroidectomy increased bone mass, an increased sensitivity of bone to parathyroid hormone in oophorectomized rats was not observed. Thus the development of the osteopenia did not seem to be related to increased parathyroid hormone sensitivity or to reduced levels of 1,25-dihydroxyvitamin D3. Exogenous 1,25-dihydroxyvitamin D3 increased bone mass in oophorectomized as well as intact rats. Intestinal calcium transport was increased by moderate doses of 1,25-dihydroxyvitamin D3. Intestinal calcium transport was also reduced by thyroparathyroidectomy and increased by the administration of parathyroid extract. A tendency for increased accumulation of 1,25-dihydroxyvitamin D3 in blood in oophorectomized rats has been noted. It is suggested that the tendency to hypercalcemia in ovarian-insufficient females given 1-hydroxylated vitamin D compounds may be related to a diminished metabolism of 1,25-dihydroxyvitamin D3.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 33 (1981), S. 129-134 
    ISSN: 1432-0827
    Keywords: Matrix vesicles ; Young/adult ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The occurrence of matrix vesicles in the maxillary bone of normal young and adult rats was demonstrated by both ultrastructural and enzymatic studies. Transmission electron microscopy revealed that the vesicles are invested in trilaminar membranes. Occasionally, crystals were found both within the vesicles and in the surrounding matrix. Separated fractions of vesicles, membranes, and cells were studied biochemically. The amounts of vesicular protein and enzymatic activity per gram bone in the adult rats were significantly lower than in the young. This coincides with the higher number of vesicles observed in the TEM specimens of young rats when compared to that in the old ones. The specific activities of all enzymes examined in the three bone fractions obtained from both young and adult rats were similar. This indicates that no significant difference exists in the enzymatic characteristics of matrix vesicles from the maxillary bone of young and adult rats.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 2 (1968), S. 60-66 
    ISSN: 1432-0827
    Keywords: Fluorides ; Topical/Pharmacodynamics ; Penicillin/Pharmacodynamics ; Calcification ; Physiologic/Drug Effects ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'objet de ce travail était de comparer le phénomène de la maturation postéruptive dans les molaires des rats ordinaires et sans germes à qui on a donné à manger un régime «non-cariogène». On a étudié les effets de l'application topique du fluorure et de la nourriture de la penicilline sur la maturation. Les molaires des rats ordinaires (nourris d'un régime «chow» — aliment mixte pour les animaux préparé d'habitude sous la forme d'une farine ou en boulettes) traités topiquement avec une solution à 1% de NaF et les molaires des rats ordinaires nourris d'un régime «chow» complété avec la penicilline à 1% ont montré un degré de maturation significativement plus grand que les molaires des rats de la même portée nourris de «chow» et traités avec de l'eau. De l'autre côté, les molaires des rats sans germes traités topiquement avec une solution à 1% de NaF n'ont montré aucune différence significative dans le degré de maturation des molaires de rats de contrôle semblables traités topiqument avec l'eau. Ces résultats sont en accord avec l'hypothèse que dans un milieu normal le procédé de minéralisation (maturation) est opposé par un procédé de déminéralisation. Le procédé de déminéralisation est un résultat de la production d'acide par des bactéries qui métabolisent les aliments encastrés dans les «sulci» des molaires. On propose que dans les animaux ordinaires le fluorure et la penicilline peuvent influencer la maturation en empêchant le procédé de déminéralisation. Dans les animaux sans germes le procédé de déminéralisation est absent parce que la microflore orale est absente.
    Abstract: Zusammenfassung Der Zweck dieser Arbeit war ein Vergleich des nach dem Durchbruch auftretenden Reifungsphänomens der Backenzähne von gewöhnlich und von keimfrei gehaltenen Ratten, die mit einer nicht-cariogenen Diät ernährt wurden. Es wurden die Auswirkungen von topisch angewendetem Fluorid, verbunden mit Penicillin-Fütterung auf die Reifung untersucht. Die Backenzähne der gewöhnlich gehaltenen Ratten (mit “Chow”-Diät ernährt), die mit einer 1%igen Na-Fluoridlösung topisch behandelt wurden, und solchen deren “Chow”-Diät zu 1% mit Penicillin versetzt wurde, zeigten einen erheblich größeren Reifungsgrad als die Backenzähne von Tieren des gleichen Wurfes, die nur mit der “Chow”-Diät und Wasser ernährt wurden. Diese Resultate stimmen mit der Hypothese überein, daß in einer normalen Umgebung dem Mineralisationsprozeß (Reifung) ein Demineralisationsprozeß entgegenwirkt. Der Demineralisationsprozeß ist durch eine Säureproduktion von Bakterien bedingt, welche die in die Zahnfurchen eingepreßten Nahrungsbestandteile metabolisieren. Es wird die Annahme vorgeschlagen, daß Fluoride und Penicillin bei den gewöhnlich gehaltenen Tieren die Reifung durch eine Inhibition des Demineralisationsprozesses beeinflussen. Bei keimfrei gehaltenen Tieren findet dagegen kein Demineralisationsprozeß statt, da die orale Mikroflora fehlt.
    Notes: Abstract The purpose of this work was to compare the phenomenon of post-eruptive maturation in molars of conventional and germfree rats fed non-cariogenic diets. The effects of topical application of fluoride and feeding penicillin on maturation were studied. The molars of conventional rats (fed a chow diet) treated topically with a 1% NaF solution and the molars of conventional rats fed a chow diet supplemented with 1% penicillin showed a significantly greater degree of maturation than did the molars of littermate rats fed chow and treated with water. On the other hand, the molars of germfree rats treated topically with a 1% NaF solution showed no significant difference in degree of maturation from the molars of similar control rats topically treated with water. These data are consistent with an hypothesis that in a normal environment the mineralization (maturation) process is opposed by a demineralization process. The demineralization process is a result of production of acid by bacteria metabolizing the diet impacted in the sulci of molars. It is proposed that in conventional animals fluoride and penicillin may influence maturation by inhibiting the demineralization process. In the germfree animals the demineralization process is absent because the oral microflora is absent.
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  • 9
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 1-11 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The distribution of the first-passage time is of decisive importance in lift (elevator) design. It yields the easure of performance of the system, the expected number of stops, the interval (i. e. time elapsed between two successive departures from a given floor) and many other parameters and factors determining the required number of lifts in a building. The derivation is based on the transition probability matrix of the operation policy, and consists in solving a system of linear equations.
    Additional Material: 3 Tab.
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  • 10
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 15 (1980), S. 55-62 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Fourier analysis of numerical accuracy has traditionally concentrated on the propagation behaviour of various methods. When systems of equations in more than one unknown are involved, analysis of propagation accuracy alone is shown to be incomplete. A distribution factor is introduced to complement the Fourier analysis in these cases, and application of the concept to two problems commonly encountered in the water resources field is demonstrated. The distribution factor is shown to provide important information which cannot be obtained from the customary analysis of propagation accuracy.
    Additional Material: 2 Ill.
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