Publication Date:
1989-12-01
Description:
Activation of spontaneously dividing T cell hybridomas induces interleukin-2 (IL-2) production, a cell cycle block, and programmed cell death. T cell hybridomas that express the T cell antigen receptor (TCR) zeta homodimer (zeta 2), but not the TCR zeta eta heterodimer, were studied. The zeta eta- cells produced little or no inositol phosphates (IP) when stimulated with antigen. In most cases the hydrolysis of phosphoinositides was also impaired after stimulation with antibody to CD3, although one zeta eta- cell produced normal concentrations of IP. The zeta eta- cells slowed their growth and secreted IL-2 in response to both stimuli. However, the zeta eta- cells did not die after activation with antigen. Since activated thymocytes also undergo programmed cell death, these results may have important implications for the role of the zeta eta.TCR in negative selection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mercep, M -- Weissman, A M -- Frank, S J -- Klausner, R D -- Ashwell, J D -- New York, N.Y. -- Science. 1989 Dec 1;246(4934):1162-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Biological Response Modifiers Program, National Cancer Institute, Bethesda, MD 20892.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2531464" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Monoclonal/immunology
;
Antigens/immunology
;
Antigens, CD3
;
Antigens, Differentiation, T-Lymphocyte/immunology
;
Cell Survival
;
*Gene Expression
;
Hybridomas/immunology
;
Inositol Phosphates/metabolism
;
Interleukin-2/secretion
;
Lymphocyte Activation/*physiology
;
Macromolecular Substances
;
Mice
;
Receptors, Antigen, T-Cell/*genetics/immunology
;
T-Lymphocytes/*immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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