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  • Artikel  (36)
  • monoclonal antibody  (23)
  • Bacteria  (11)
  • Lepidoptera
  • temperature
  • Springer  (36)
  • 1990-1994  (36)
  • Werkstoffwissenschaften, Fertigungsverfahren, Fertigung  (36)
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  • Artikel  (36)
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  • 1
    Digitale Medien
    Digitale Medien
    Cadmium sorption by bacteria and freshwater sediment (1991)
    Springer
    Journal of industrial microbiology and biotechnology 8 (1991), S. 201-207 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Diffusion chamber ; Cadmium-sensitive ; Cadmium-resitant ; Sediment ; Bacteria ; Cadmium-sorption
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Sorption of cadmium by sediment bacteria and freshwater sediment was investigated using diffusion chambers to simulate the water-sediment interface. Diffusion chambers were constructed to provide two compartments separated by a dialysis membrane. Diffusion of cadmium across the membrane was monitored after pure cultures of sediment bacteria or lake sediments were added to the sediment side of a diffusion chamber. Cellular accumulation of cadmium by cadmium-sensitive and cadmium-resistant bacteria removed between 20% and 80% of the dissolved cadmium from the simulated water column and pore water. Cellular accumulation of cadmium was greatest for cadmium-sensitive isolates that were tested. Sediment with an intact microbial community sequestered 80% of the cadmium added to sediment, whereas autoclaved sediment retained 97% of the metal that was added. Addition of glucose to cadmium-amended sediment decreased retention of cadmium by untreated and autoclaved sediments, resulting in elevated concentrations of dissolved cadmium in the simulated water column.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01575854
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  • 2
    Digitale Medien
    Digitale Medien
    Oligotrophic bacteria in ultra-pure water systems: Media selection and process component evaluations (1991)
    Springer
    Journal of industrial microbiology and biotechnology 8 (1991), S. 223-227 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Deionized water ; Ultra-pure water ; Ozone ; Ultra-violet sterilization ; Oligotroph ; Bacteria ; R2A medium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Presently, tryptic soy agar (TSA) medium is used in the semiconductor industry to determine the concentration of viable oligotrophic bacteria in ultra-pure water systems. Deionized water from an ultra-pure water pilot plant was evaluated for bacterial growth at specific locations, using a non-selective medium (R2A) designed to detect injured heterotrophic as well as oligotrophic bacteria. Results were compared to those obtained using Tryptic Soy Agar. Statistically greater numbers of bacteria were observed when R2A was used as the growth medium. Total viable bacterial numbers were compared both before and after each treatment step of the recirculating loop to determine their effectiveness in removing bacteria. The reduction in bacterial numbers for the reverse osmosis unit, the ion exchange bed, and the ultraviolet sterilizer were 97.4%, 31.3%, and 72.8%, respectively, using TSA medium, and 98.4%, 78.4%, and 35.8% using R2A medium. The number of viable bacteria increased by 60.7% based on TSA medium and 15.7% based on R2A medium after passage of the water through an in-line 0.2-μm pore size nylon filter, probably because of the growth of bacteria on the filter. Our results suggest that R2A medium may give a better representation of the microbial water quality in ultra-pure water systems and therefore a better idea of the effectiveness of the various treatment processes in the control of bacteria.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01576059
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  • 3
    Digitale Medien
    Digitale Medien
    Bacterial conjugation betweenEscherichia coli andPseudomonas spp. donor and recipient cells in soil (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 79-84 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Bacteria ; Soil ; Conjugation ; Gene transfer ; Plasmids ; Survival
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Experiments conducted in microcosms containing loam soil samples inoculated with eitherE. coli orPseudomonas spp. donor and recipient cells showed that bacterial cells survived and conjugated over a 24-h incubation period.E. coli transconjugants were detected 6 h after donor and recipient strains were introduced into sterile soil samples. In non-sterile soil samples, transconjugants were detected between 8 and 24 h incubation.Pseudomonas transconjugants were recovered from sterile soil samples between 6 and 12 h after their introduction and as early as 2 h in non-sterile soil. The results show that genetic interactions occur in non-sterile soil in relatively short periods of time at relatively high transfer frequencies (10−3 to 10−4). Studies on genetic interactions in soil are becoming necessary in risk assessment/environmental impact studies prior to the release of genetically engineered or modified organisms into uncontained environments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01573856
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  • 4
    Digitale Medien
    Digitale Medien
    Production of extracellular emulsifying agent byPseudomonas aeruginosa UG1 (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 25-31 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Oil ; Emulsifier ; Bacteria ; Pseudomonas aeruginosa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Twenty-three bacterial strains were isolated from oil-contaminated soil samples. Of these, 20 displayed some ability to effect oil dispersion and they were screened quantitatively for the ability to emulsify 0.5% (v/v) reference oil. One strain, identified asPseudomonas aeruginosa UG1, produced extracellular material that emulsified reference oil, hexadecane and 2-methylnaphthalene at concentrations as high as 6% (v/v) in nutrient broth. Emulsification activity increased during a 10 day incubation period at 30°C. The activity was not influenced by pH over the range 5 to 9. The emulsifying agent was precipitated by cold ethanol. The highest emulsifying activity was detected in the extracellular fraction precipitated between 30 and 50% (v/v) ethanol. A linear relationship was observed between emulsifier concentration (mg/ml) and emulsifying activity. Genetic analysis showed that thePseudomonas aeruginosa UG1 strain did not carry extrachromosomal plasmids, suggesting that the gene(s) coding for emulsifying activity was carried on the chromosome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01569603
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  • 5
    Digitale Medien
    Digitale Medien
    Bacterial species in raw and cured compost from a large-scale urban composter (1994)
    Springer
    Journal of industrial microbiology and biotechnology 13 (1994), S. 177-182 
    Details
    ISSN: 1476-5535
    Schlagwort(e): Antibiotic resistance ; Bacteria ; Compost ; Metal-resistance ; Thermophilic bacteria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Summary Raw and cured compost samples from a large-scale urban composter were studied over a period of eight months to gain information on bacterial species present. Total viable, aerobic heterotrophic bacteria, lactose-positive bacteria, antibiotic and metal-resistant bacteria and thermophilic bacteria were enumerated. Both raw and cured compost samples contained metal and antibiotic-tolerant bacteria (〈Log 3.0 to Log 8.5 CFU g−1 compost) as well as high numbers (as high as Log 7.4 CFU g−1 dry weight compost) of thermophilic bacteria isolated by growth at 55 °C. Selected colonies were also identified using the Biolog 95 substrate identification system.Escherichia coli andSalmonella spp. were not detected in compost samples.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01584004
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  • 6
    Digitale Medien
    Digitale Medien
    A serum-free medium for hybridoma cell culture (1993)
    Springer
    Cytotechnology 11 (1993), S. 169-174 
    Details
    ISSN: 1573-0778
    Schlagwort(e): cell culture ; hybridoma ; monoclonal antibody ; serum-free medium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The effects of several different substances, including insulin, transferrin, ethanolamine, selenite and butyrate on the growth of murine hybridoma 2F7 cells, which secrete monoclonal antibody against small cell lung cancer, were investigated, and a serum-free medium SFMI was formulated. The effects of taurine, spermidine, progesterone and adenine on the cell growth were tested further on the basis of the medium SFMI, and a modified serum-free medium SFM II was established. On the basis of medium SFM II, the substitution tests of ferric citrate for transferrin were carried out, and it was found that transferrin could be replaced. The experiments suggested that the formulated serum-free medium was suitable for 2F7 cell growth and monoclonal antibody secretion, and thus facilitated subsequent purification of monoclonal antibody.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00749866
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  • 7
    Digitale Medien
    Digitale Medien
    Electron microscopy of hybridoma cells with special regard to monoclonal antibody production (1990)
    Springer
    Cytotechnology 4 (1990), S. 13-28 
    Details
    ISSN: 1573-0778
    Schlagwort(e): monoclonal antibody ; hybridoma ; electron microscopy ; endoplasmic reticulum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00148807
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  • 8
    Digitale Medien
    Digitale Medien
    Antibody production in packed bed reactors using serum-free and protein-free medium (1990)
    Springer
    Cytotechnology 4 (1990), S. 279-283 
    Details
    ISSN: 1573-0778
    Schlagwort(e): animal cell culture ; hybridoma ; monoclonal antibody ; packed bed reactor ; continuous culture ; perfusion ; protein-free medium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The present work demonstrates the utility of packed bed reactors for the production of monoclonal antibody. We present data from a continuous process run for the production of over 100 grams of antibody, using serum-free medium. An additional pilot run also demonstrates the potential for continued antibody production under protein-free conditions, using a standard basal medium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00563788
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  • 9
    Digitale Medien
    Digitale Medien
    Cell cycle kinetics of the accumulation of heavy and light chain immunoglobulin proteins in a mouse hybridoma cell line (1994)
    Springer
    Cytotechnology 14 (1994), S. 205-218 
    Details
    ISSN: 1573-0778
    Schlagwort(e): Cell cycle ; flow cytometry ; heavy chain ; hybridoma ; light chain ; monoclonal antibody ; population balance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Rates of accumulation of immunoglobulin proteins have been determined using flow cytometry and population balance equations for exponentially growing murine hybridoma cells in the individual G1, S and G2+M cell cycle phases. A producer cell line that secretes monoclonal antibodies, and a nonproducer clone that synthesizes only κ-light chains were analyzed. The pattern for the kinetics of total intracellular antibody accumulation during the cell cycle is very similar to the previously described pattern for total protein accumulation (Kromenaker & Srienc 1991). The relative mean rate of heavy chain accumulation during the S phase was approximately half the relative mean rate of light chain accumulation during this cell cycle phase. This indicates an unbalanced synthesis of heavy and light chains that becomes most pronounced during this cell cycle phase. The nonproducer cells have on average an intracellular light chain content that is 42% lower than that of the producer cells. The nonproducer cells in the G1 phase with low light chain content did not have a significantly higher rate of light chain accumulation relative to other G1 phase nonproducer cells. This is in sharp contrast to what was observed for the G1 phase producer cells. In addition, although the relative mean rate of accumulation of light chain was negative for G2+M phase nonproducer cells, the magnitude of this relative mean rate was less than half that observed for the producer cells in this cell cycle phase. This suggests that the mechanisms that regulate the transport of fully assembled antibody molecules through the secretion pathway differ from those which regulate the secretion of free light chains. The results reported here indicate that there is a distinct pattern for the cell cycle dynamics of antibody synthesis and secretion in hybridomas. These results are consistent with a model for the dynamics of secretion which suggests that the rate of accumulation of secreted proteins will be greatest for newborn cells due to an interruption of the secretion pathway during mitosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00749617
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  • 10
    Digitale Medien
    Digitale Medien
    Methods and strategies available for the process control and optimization of monoclonal antibody production (1994)
    Springer
    Cytotechnology 14 (1994), S. 219-232 
    Details
    ISSN: 1573-0778
    Schlagwort(e): animal cells ; cellular metabolism ; cultivation ; hybridomas ; modelling ; monoclonal antibody ; process control ; optimisation ; simulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The objective of this paper is to explore the range of methods and strategies available for the process control and optimization of monoclonal antibody production by hybridoma cell culture. Emphasis will be placed on the choice of the level of complexity incorporated into the process control and optimisation procedure. It will be shown that the behaviour of hybridomas in culture is influenced by sophisticated cellular metabolic activities and various interactive environmental factors and that the understanding and modelling of the way hybridomas grow in the bioreactor should enable optimisation of bioreactor operating conditions to achieve maximum monoclonal antibody formation. However, due to the lack of on-line instrumentation of important biological variables and the incomplete knowledge of hybridoma cultivation process, there exist many limitations and challenges to the advent of applications of process control and optimisation in this field. To solve the problem, introduction of industrially practical biological measurements and development of new control concepts are inevitable. At the end of this paper, we shall discuss possible schemes for the control of the physsiological state of cells in order that balanced cell growth and maximum monoclonal antibody synthesis may be achieved.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00749618
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