ALBERT

Notes: A breccia vein sampled from a shear zone in greenschist facies metapelites at Mount Isa, Queensland, Australia, shows a systematic variation in vein geometry that is related to the geometry of folding and faulting within the sample. Calcite vein-fill is coarse grained and equigranular, suggesting precipitation in a fluid-filled space. Partially folded veins suggest that veining occurred during folding and faulting. The breccia vein contains a central zone in which dilation has occurred simultaneously in all directions in the plane of section, implying that this was a zone of high fluid pressure and nearly isostatic differential stress during folding and faulting. From these observations, it can be inferred that the breccia vein was a zone of high permeability and a likely fluid channel during deformation. This hypothesis was tested by stable isotope analysis of veins and host rocks. The calcite veins have δ13C values of -11.1 ± 0.1% and δ18O values of 6-10%o, whereas the host metapelite has δ13C values of -10.62 and -10.11% and δ18O values of 14-15%o. These values are consistent with an igneous-derived, H2O-dominated fluid that exchanged little oxygen with the host rocks, but derived much of its carbon from the wall rock. The isotopic disequilibrium between the veins and the wall rock confirms that the fluid was externally derived, and that the breccia vein acted as a channel for large-volume fluid flow within the shear zone.

Notes: This paper describes the progressive metamorphism and deformation of a series of metasediments, Le Conquet Schists and their higher grade equivalents, which occur as tectonically emplaced screens within a sequence of foliated gneisses, the Gneiss de Brest and Gneiss de Lesneven. The sequence exhibits a steep south to north increase in metamorphic grade from garnet-staurolite schist to sillimanite gneiss and sillimanite-K-feldspar migmatite. The relationship of mineral growth to foliation development has been established for individual screens. At least five phases of deformation (D1-D5) are preserved. Analysis of porphyroblast inclusion trails is used to demonstrate sequential mineral growth during the successive development of orthogonal foliations S1-S4. Porphyroblasts continued to grow during the subsequent development of C-S mylonite fabrics and extensional crenulation cleavages which are genetically related to a series of high-strain zones (D5). Mineral assemblages, phase relations and mineral chemistry are consistent with porphyroblast growth being the result of continuous reactions. Microstructure-porphyroblast relations are used to show that although mineral growth proceeded during continuous reactions, these only operated episodically. Phase relations, mineral chemistry and P-T estimates are used to constrain P-T trajectories and these are linked to the deformation histories within individual screens. A comparison between the resulting pressure-temperature-deformation paths is used to demonstrate that the metamorphic peak occurred progressively later and at successively lower pressures with increasing metamorphic grade. It is suggested that the early evolution of the belt is the result of crustal thickening by overthrusting. The subsequent history is one of progressive heating and unroofing of the higher grade rocks in a dextral strike-slip transtensional shear zone.

Notes: Porphyroblast inclusion trails have the potential to provide critical information about tectonometamorphic events. Recently, however, traditional interpretations of inclusion trails have been called into question by the suggestions that porphyroblasts do not rotate during non-coaxial deformation and that apparent spiral inclusion trails can be generated in coaxial deformation. We present a new computer model that simulates inclusion trail development. Model results suggest: (1) that the extent of porphyroblast rotation is controlled by conditions at the porphyroblast-matrix boundary; (2) that curved inclusion trails may develop in unrotated porphyroblasts; (3) that classic “snowball” inclusion trails are most simply explained by rotational growth histories; and (4) that some of the observations used to support the view that porphyroblasts do not rotate (e.g. weakly sigmoidal inclusion trails, apparent truncations of inclusion trails) can be accounted for by variations in the growth rate of rotating porphyroblasts.

Notes: The feasibility of measuring soil salinity with electromagnetic induction (EM) for determining riparian restoration potential was investigated on a 28-hectare plot at the Bosque del Apache National Wildlife Refuge in central New Mexico. The plot was cleared of exotic Tamarix chinensis (saltcedar), surveyed and gridded into 1370.2 hectare sections. Soil samples and EM measurements were taken at each section. We compared laboratory-determined ECe values from the soil samples with ECa values calculated from the EM measurements using a model developed by Rhoades et al. (1990). Direct comparison of ECe values determined from the two methods yields a low correlation due to sample-size differences but the calculated ECa was able to accurately predict whether the measured ECe would lie above or below some threshold value. An assessment of general site suitability for riparian restoration with electromagnetic induction has proven to be a rapid, accurate, and cost-effective alternative to intensive soil sampling.

Notes: Extensive areas of the tropics have been converted into pasture for cattle ranching. Frequently, abandoned pasture does not revert to forest. The goal of this project was to identify barriers to lowland moist forest regeneration in highly degraded grasslands in the Sierra Nevada de Santa Marta, Colombia. The barriers we considered were seed source, seed predation, competition with grasses, microclimate and soil limitations on plant growth, and fire. Seed dispersal into the grasslands is limited to within 10 meters of forest fragments, but this barrier can be overcome by sowing seeds and planting seedlings and by establishing perches to attract dispersers. In these degraded grasslands, seed predation was lower than in the adjacent forest patches, and there was no evidence that grasses inhibited the establishment of woody species. The most important barrier was the severe degradation of the soils. In much of the area, the A and B horizons have been eroded away, leaving saprolite at the soil surface. Seedlings of two fast-growing pioneer species, Ochroma pyramidale and Cochlospermum vitifolium, grew to a maximum height of only 2.5 and 12 cm, respectively, during the first eight months. The slow plant growth in the degraded grassland soils compared to forest soils was associated with lower levels of cation-exchange capacity, calcium, magnesium, and potassium. Even if these barriers could be overcome, the frequent and extensive use of fire in the region must be controlled to avoid killing established woody plants.

Notes: Biological resources can be more usefully incorporated into many aspects of restoration ecology. During the planning and design stage, the wide genotypic variation in natural plant populations must be recognized and exploited. This will ensure that genotypes used on a site are best adapted to local conditions and have a greater probability of survivorship than arbitrarily chosen material. Also, certain unusual genotypes can be located using the principles of evolutionary ecology and can be installed in areas with extreme conditions, such as soils contaminated with heavy metals, in areas where rapid colonizing ability (high seed set and/or clonal growth) is particularly advantageous, or where soils are of poor quality. Similarly, where high herbivore pressure is a threat to restoration, genotypes that are well defended, chemically or mechanically, against animal enemies should be selected to initiate the restoration process. The nursery industry can be encouraged to supply an ecologically wider selection of material for restoration, originating from local biological reserves and natural habitats. During the management phase of a restoration, local natural habitats are critical as reservoirs of biological control agents, seed sources for plant species, and members of higher trophic levels and additional plant species needed during succession. Mutualists such as pollinators, seed dispersers, and mycorrhizal fungi are vital to the success of a restoration project, and these must invade from nearby natural habitats or must be deliberately introduced. During the evaluation phase of restoration, local natural areas should be used as templates of community composition and structure from which one measures success. A functioning restoration project will interact biologically with surrounding areas, the exchange of species and genes being particularly important. Analysis of the microbial and invertebrate communities that have invaded the installed plant community may be useful and accurate determinants of ecological function. For these latter stages of the restoration process, the value of preserving local habitat remnants is high and complements their usefulness as a source of ecologically precise material for installation.

Notes: Community classification has emerged from ecology textbooks into the arena of environmental impact evaluation and mitigation. This development is especially apparent in southern California, where the fate of a community called coastal sage scrub is being decided. Regional plans for development, mitigation, preservation, and restoration are being formulated that will permanently affect the natural landscape. This paper demonstrates the potential for the name of a plant community to affect tradeoffs in planning processes that are intended to offset removal of habitat by development. The discussion focuses on two types of classification systems. One system is hierarchical, with established nomenclatorial rules that allow natural variation to refine the community definitions. Names of plant associations directly reflect the dominant species in the association, as in Artemisia californica—Eriogonum fasciculatum (California sagebrush—California buckwheat). The second system is nonhierarchical, in which observations of natural landscapes are fit into established definitions of community types, such as Diegan coastal sage scrub. Advantages and disadvantages of both types of systems are discussed and illustrated by two examples of problems in classifying coastal sage scrub. The discussion concludes with the point that the name of a community has the potential to significantly affect the extent to which equal tradeoffs between community types are actually achieved in mitigation and restoration efforts.

Notes: This article contributes to the worldwide trend of using microbiological methods for bioindication of the influence of abiotic, biotic, and anthropogenic factors on the soil environment, especially on the plough layer of soils. It compares two approaches to assess microbial biomass-C: (1) chemical treatment of soil samples by CHCl3 vapor, followed by extraction, and (2) heat treatment in the range of 62–65°C for 24 hours, followed by extraction. It recommends the analysis of different forms of organic carbon, the total carbon of soil organic mass, and microbial biomass-C and other forms of organic carbon extractable with 0.5 M K2SO4 and 0.5 M (NH4)2SO4 respectively. It presents a simple mathematical model suitable for assessment of microbial recuperation of the topsoil (0–15 cm) after restoration. The relation between the measured values and the values calculated with the model expressed as percentages expresses the quality of restoration achieved and the stage of disturbance of the living microbial part of the topsoil. It indicates not only the ecological impact but also the deficit and stress situations, to which the living microbial part of the topsoil is exposed in the long term.

Notes: Books reviewed in this article: Ecological Principles of Nature Conservation. L. Hansson, editor Saltmarsh Ecology. P. Adam

Notes: Within an urban park in southern California, the relationship between the structure and floristics of vegetation and the distribution, abundance, and behavior of wildlife was studied in relatively undisturbed areas (San Luis Rey) and in contiguous areas (Guajome Park) in need of restoration. These data were used to develop recommendations for the enhancement of native animal species in the park. The abundance of amphibians and reptiles was highest in native upland scrub and willow (Salix)-riparian vegetation types, and lowest in dry, disturbed sites. Western fence lizards (Sceloporus occidentalis) were the most abundant reptile throughout both study areas. Overall, bullfrogs (Rana catesbiana), an exotic species, were the dominant amphibians; the native Pacific treefrog (Hyla regilla) was rare throughout. At both study areas, the small mammal community was dominated by western harvest mice (Reithrodontomys megalotis) and deer mice (Peromyscus maniculatus) and, to a lesser extent, by brush mice (P. boylii) and exotic house mice (Mus musculus). Negative correlations in abundance existed between house mice and harvest mice, and between house mice abundance and overall small mammal abundance. In riparian sites, cottonwood (Populus fremontii) and various height classes of willow were the dominant factors in the majority of bird abundance–habitat‘correlations and where foraging activities were concentrated. Recommendations for enhancing native animal species include reduction of marsh sedimentation, removal of feral species, and development of connections between the park and nearby natural areas. A corridor of native riparian vegetation (primarily cottonwood-willow) should be developed to replace the existing agricultural fields, thereby linking Guajome with the San Luis Rey River.

Notes: Eight individual rock iguanas (Iguana pinguis) from Anegada Island were relocated to Guana Island by Lazell, 1984–1987, in order to establish a second population reservoir for this endangered species. The species may have originally occupied the entire Puerto Rico Bank. The relocation has been successful and, in the area currently providing the best habitat, we estimate a density of 9 or 12 animals of various age classes per 19 ha. The optimal area contains a sheep exclosure with relatively dense understory vegetation and numerous exotic as well as native species of plants. Iguana activity is concentrated on east facing slopes and ridge-tops that get morning sun. Outside the exclosure most edible ground cover and shrubs have been eaten by sheep, leaving toxic or noxious species, such as Croton or Lantana, in the understory where I. pinguis adults generally forage. Removal of sheep may be critical to continued population growth of these reptiles. Views on relocation or repatriation of other endangered Antillean Iguana species are advanced, with some ideas on minimum viable population sizes and a possible explanation for the extirpation of I. pinguis from much of its former range.

Notes: Books reviewed in this article: Functions of Nature Evaluation of Nature in Environmental Planning, Management and Decision Making. Rudolf S. de Groot Landscape Restorastion Handbook D. Harker, S. Evans, M. Evans, and K. Harker

Notes: While the economic approach to the politics of regulation emphasizes the importance of organized economic interests in shaping policies, political institutions in which regulatory agencies are embedded may also have significant effects. By including both economic influences and characteristics of political institutions in a model of price setting by state regulators, this paper demonstrates that both shape regulatory behavior in the telecommunications industry. Whether commissioners are elected or appointed, whether they face confirmation by a legislature, and whether a single party controls both executive and legislative branches of state governments influence the level of prices charged for basic services.

Notes: We investigate the choice of compensation scheme by firms. Our basic model shows that the unique equilibrium choice for profit maximizing duopsonists in a labor market is for one firm to offer a wage rate and for the other to offer a piece rate. This result arises because the firms recognize that, by offering different compensation schemes, they induce self-selection among workers, which thereby decreases the intensity of competition in the labor market. We find this asymmetry to be robust to allowing for firing, free entry, and a class of more general compensation schemes. When we broaden our model to permit firms to be differentiated in the eyes of workers (either geographically or by “other working conditions,” e.g.), we find that our results are preserved when differentiation is low, but that both firms choose to offer a piece rate when differentiation is high.

Notes: In the present study I identify an inherent characteristic of health care markets that may lead to excessive investment by hospitals even when compensated according to a prospective reimbursement rule. It is demonstrated that the stochastic nature of the demand for medical services combined with the lumpiness of investment decisions may give rise to excessive investment when multiple hospitals select independently their levels of capacities. The source for the excessive incentives to invest is the difficulty of one hospital to internalize properly the externality generated by its investment decisions. Such an externality arises because when one hospital expands its capacity, it is more likely to be able to serve not only patients residing in its region but patients residing in neighboring regions as well.

Notes: What is the nature of the industrial organization of the market for physician services? Is the market “competitive?” Are there pareto-relevant market failures, such that there is room for welfare-improving policies? Economists have devoted a great deal of attention to this market, but it remains relatively poorly understood. The key features of this market are that the product being sold is a professional service, and the pervasive presence of insurance for consumers. A professional service is inherently heterogeneous, nonretradable, and subject to an asymmetry of information between buyers and sellers. These characteristics are what bestow market power on sellers, further strengthened by the fact that consumers face only a small fraction of the price of any service due to insurance. This paper considers the implications of these characteristics for agency relationships between patients and physicians, and insurers (both private and public) and physicians. Agency relationships within physician firms are also considered. Both theoretical and empirical modeling of contracting between insurers and physicians and of the joint agency problems between patient and physician and insurer and physician are recommended as areas for future research. Because failures in this market are seen to derive largely from the structure of information, the potential gains from government intervention may be sharply circumscribed. Nonetheless, careful consideration of the competitive implications of contracting between physicians, insurers, and other health care providers is an important area for antitrust policy.

Notes: Books reviewed in this article: Economics, Organization and Management, by Paul Milgrom and John Roberts

Notes: The publication of the passive learning model of Jovanovic and subsequently the active exploration model of Ericson and Pakes led to a resurgence in research on firm growth and survival. Empirical work measuring the relationship of growth and survival relative to size and age uncovered a number of strong regularities, consistent with the new theoretical models and also with a rather broad class of models of which the above represent special cases. But recent theoretical work has shown that when the learning models are applied to the special case of the labor managed firm, most of the sharp empirical predictions derived for profit maximization are rendered indeterminate. Given the impact of ownership structure on the theoretical results of the learning models, we use a dataset of French producer cooperatives to determine whether firms of different ownership structures actually have substantially different growth and survival patterns. We find that French cooperatives survive and grow in very much the same way as has been found for U.S. firms, suggesting that the empirical regularities previously uncovered hold for a much broader class of firms. We are left to conclude that the underlying empirical growth and survival relationships continue to hold for firms with sharply differing ownership structures, and the theoretical models are attempting to explain these broad-based relationships by means of overly specific modeling.

Notes: This paper uses origin-destination data to define geographic local hospital markets in large metropolitan statistical areas (MSAs). Results support past findings of service rather than price competition, with negative-cost Herfandahl-Hirschman indexes relationships at the market level (and sub-MSA level) and with profit margins negatively related to hospital market competition. The effects of total (direct plus indirect) market competition are unchanged between 1983 and 1988, precisely estimated, robust to estimation techniques, and unaffected by whether the number of direct competitors is included as an explanatory variable.

Notes: Users of photogrammetric data have often been puzzled by conflicting advice given by companies regarding achievable height accuracies which could be expected from varying scales of aerial photography. The author chairs the Photogrammetry Group which is convened under the auspices of the Council of the UK Land and Hydrographic Survey Association (TSA). The first aim of this Group was to establish some common ground among TSA member companies with regard to achievable heighting accuracies. A series of tests was carried out on the most commonly used photographic scales and guidelines were published to clients based on these tests. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé Les utilisateurs des données photogrammétriques ont été souvent déconcertés par les avis contraires émanant des entreprises concernant les précisions altimétriques accessibles à partir de diverses échelles des photographies aériennes. Dans ce contexte, le Conseil de ľ‘Association britannique des levées terrestres et hydrographiques (TSA) a mis sur pied un Groupe “Photogrammétrie” et en a confié la présidence àľauteur. Le premier objectif de ce Groupe a consistéàétablir des bases communes aux entreprises membres de la TSA, en ce qui concerne les précisions altimétriques réalisables. On a effectué un ensemble ď essais sur les échelles des photographies aériennes les plus communément utilisées et ľ on a publicé, à partir de ces essais un opuscule destinéà guider les clients. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die Nutzer photogrammetrischer Daten werden oft durch widerspriich-liche Ratschläge der Firmen in bezug auf die bei verschiedenen Bildmaβstäben erreichbare Höhengenauigkeit verwirrt. Der Autor leitet den photogrammetrischen Ausschuβ, der unter Aufsicht des Council des UK Land and Hydrographic Survey Association (TSA) einberufen wurde. Erstes Ziel dieses Gremiums war es, eine gemeinsame Grundlage unter den TSA-Mitgliedsgesellschaften im Hinblick auf erreichbare Höhengenauigkeit zu schaffen. Es wurde eine Reihe von Versuchen zu den am meisten verwendeten Bildmaβstäben durchgeführt, undes wurden daraufberuhende Richtlinien für Kunden veröffentlicht.

Notes: Cartography andphotogrammetry have always been strongly linked and this paper emphasises a view of cartography as a process of spatial data representation, rather than as a method of creating a product, a map. Of course, the definition of cartography must change with developments in its practice. An outline of the historical background to the subject is presented, followed by a consideration of the relationship between the cartographer and the user of the product. The basic problems involved in the transfer of information from the complex real world to a generalized cartographic product are analysed and technological aspects of the discipline are described. Contemporary applications of cartography include hazard mapping, links with GIS, tactile products and electronic hydrographic representation, all of which represent customer driven progress. It is concluded that the future of cartography is, overall, dependent on a graphical representation of high quality being supplied to the end user, rather than on the methods used to attain this objective. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé La cartographie et la photogramétrie ont toujours été liées étroitement ľune àľautre, et ľ‘on souligne dans cet article ľ‘idée que la cartographie est un systéme de représentation des données à trois dimensions, plutôt qu‘un ensemble de procédés de fabrication ďun produit, la carte. II est évident que la définition de la cartographie doit changer au fur et à mesure que des évolutions apparaissent dans sa pratique. Aprés un aperçu du contexte historique de ce thème, on examine les relations qui se développent entre le cartographe et ľutilisateur du produit. On analyse les problèmes fondamentaux du transfert ďinformations du monde réel, complexe, à un produit cartographique géneralisé et ľ‘on décrit la technologie qu'utilise cette discipline. Les applications récentes de la cartographie concernent la cartographie des risques naturels, les relations avec les SIG, les cartes qui se lisent par le toucher, et la représentation electronique de ľ‘hydrographic produits qui tous sont issus de cheminements pilotés en aval, par le client. On en déduit que la cartographie est, àľ'avenir, par dessus tout fonction ďune represséntation graphique de haute qualityé fournie à un utilisateur final, plutôt que de processus utilisé pour parvenir à cet objectif.〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Kartographie und Photogrammetrie waren immer eng verbunden, und dieser Beitrag betont starker eine Auffassung von der Kartographie als einem Prozeβ der Darstellung von Raumdaten als ein Verfahren zur Herstellung eines Erzeugnisses, der Karte. Natürlich muβ sich die Definition der Kartographie bei Entwicklungen in ihrer Praxis ändern. Es erfolgt eine Skizzierung des historischen Hintergrundes des Gegenstands, gefolgt von Betrachtungen zur Beziehung zwischen Kartographen und dem Nutzer des Erzeugnisses. Die mit der Übertragung von Informationen aus der komplexen realen Welt in ein generalisiertes kartographisches System verbundenen grundlegenden Probleme werden analysiert, und es werden technologische Aspekte der Disziplin beschrieben. Zeitgenössische Anwendungen der Kartographie schlieβen die Kartierung von Katastrophen, Verbindungen zu GPS, gefühlsmäβge Erzeugnisse und elektronische hydrographische Darstellungen ein, die alle den vom Nutzer beeinflufiten Fortschritt darstellen. Es wird geschluffolgert, daft die Zukunft der Kartographie vor allem von der graphischen Darstellung hoher Qualität, die dem Endnutzer geliefert wird, abhängig ist undnicht so sehr von den Methoden, me dieses Ziel erreicht wird.

Notes: Since its foundation in 1982, the Institut Cartogràfic de Catalunya has been dealing with digital images. Though digital images and techniques were initially confined to remote sensing, they have been gradually transferred to and combined with photogrammetry. In addition to the development of remote sensing and digital orthophotography systems, this expertise has facilitated the introduction of commercially available digital photogrammetric systems (scanning and stereocompilation) which are now in full operation. The paper describes the experience in scanning, orthophotography production and stereocompilation, draws some conclusions and gives an insight of the future of digital photogrammetry. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé L‘Institut Cartographique de Catalogue s‘est intéressé au traitement des images numériques depuis le moment où il a été crée, en 1982. Bien qu'au début les images numériques et leur technologie aient été réservées à la télédétection, elles se sont trouvées peu à peu transférées et intégrées à la photogrammétrie. Ce savoir-faire a permis le développement des systèmes de télédétection et ď orthophotographie numérique et a, de plus, facilité la mise à disposition sur le marché de systèmes de photogrammétrie numérique qui sont maintenant pleinement opérationnels. On présente dans cet article ľ expérience acquise dans le domaine du balayage, de la production des orthophotographies et de la stéréocompilation, on en tire quelques conclusions et ľ on termine par un aperçu sur ľ‘avenir de la photogrammétrie numérique. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Seit seiner Gründung im Jahre 1982 hat sich das Institut Cartogràfic de Catalunya mit digitalen Bildern beschäftigt. Obwohl digitate Bilder und Verfahren ursprünglich auf die Fernerkundung begrenzt waren, wurden sie schrittweise auf die Photogrammetrie übertragen bzw. mit ihr kombiniert. Zusätzlich zur Entwicklung von Fernerkundung und digitaler Orthophoto-Systeme hat diese Erfahrung die Einführung kommerziell verfügbarer digitaler photogrammetrischer Systeme erleichtert, die jetzt überall in der Praxis angewendet werden. Der Beitrag beschreibt die Erfahrung bei der Abtastung, der Herstellung von Orthophotos und bei der Stereoauswertung und gibt einige Schluβfolgerungen und Aussichten in bezug auf die Digitalphotogrammetrie wieder.

Notes: The paper entitled The Photogrammetric Society Analogue Instrument Project was published in The Photogrammetric Record, 14(82): 565–582 (October 1993) and contained, as Appendix A, an extract from the Archive concerned with the history of the Zeiss Stereoplanigraph in Great Britain. In The Photogrammetric Record, 14(83): 769–782 (April 1994), three further Appendices were published which dealt with the Williamson Multiplex equipment and other instruments. In this third extract, the history of five other instruments of the Multiplex type are described. This brings to a close that section of the Archive dealing with instruments using direct optical projection.〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On a publicé dans le numéro 14(82) (pp 565–582) ďoctobre 1993 du“Photogrammetric Record”ľarticle intitulé“le projet de la Société (britannique) de Photogrammétrie sur les instruments analogiques” et inséré dans ľannexe A un extrait des Archives relatif a ľhistorie du Stereoplan-igraphe Zeiss en Grande-Bretagne. On a publicé dans le numéro 14 (83) ďavril 1994 du“Photogrammetric Record”(pp 769–782) trois annexes supplémentaires qui traitaient de ľappareil Multiplex de Williamson et ďautres instruments. Dans ce troisième extrait, on présente ľhistorie de cinq autres appareils du type Multiplex. Cette partie des Archives qui traitait des appareils basés sur ľemploi ďuneprojection optique directe, se trouve ainsi achevee.〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Der Beitrag mit dem Titel“The Photogrammetric Society Analogue Instrument Project”wurde im Photogrammetric Record, 14(82): 565–582 (Oktober 1993) veröffentlicht und enthielt als Anlage A einen Extrakt vom Archiv, das mit der Geschichte des Zeiss Steroplanigraph in Groβbritannien verbunden war. Im Photogrammetric Record, 14(83); 769–782 (April 1984) wurden 3 weitere Anlagen veröffentlicht, die vom Williamson Multiplex und anderen Geräten handelten. In diesem 3. Auszug wird die Geschichte von 5 anderen Geräten des Multiplex-Typs beschrieben. Das führt zum Abschluβ desjenigen Teils des Archivs, das sich mit Geräten mit direkter optischer Projektion befaβt.

Notes: An analysis is given of the modelling of XY stage errors in an analytical plotter by polynomial functions. This is discussed as an alternative to demanding strict mechanical accuracy in the stage construction. In an era of plentiful computational resources, it is concluded that apportioning more of the responsibility to the mathematical co-ordinate refinement is an effective strategy. A second degree polynomial is used for each of the two stages in an analytical plotter, modelling the systematic stage errors to within the variability of a single observation. A requirement for instrument stability is emphasized. Such techniques have application for other instruments such as image scanners. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On étudie la modélisation des erreurs des chariots XY ďun restituteur analytique en utilisant des fonctions polynomials. II s‘agit en fait de trouver une alternative à des exigences de prêcision mêcanique très strictes dans la réalisation de ces chariots. A une époque oùľon dispose ďune abondance de ressources en moyens de calcul, il apparait en effet relever ďune stratégie efficace de s'appuyer davantage sur les traitements mathématiques pour améliorer les valeurs des coordonnées. On utilise, pour chacun des deux chariots, un polynome du second degrè pour modéliser les erreurs systématiques des chariots, et les rendre inférieures aux erreurs propres à une observation unique. On insiste sur la nécessitéďune grande stabilityé instrumental. Ces processus peuvent également s'appliquer àďautres instruments comme les scanneurs ďimages. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Es wird die Modellierung von Fehlern des xy-Wagens bei analytischen Auswertegeräten mit Hilfe von Polynomen analysiert. Des wird als Alternative zur Forderung nach einer strengen mechanischen Genauigkeit bei der Bildwagenkonstruktion diskutiert. Im Zeitalter hervorragender Computer-Ressourcen, so wird geschluβfolgert, ist es eine effektive Strategie, sich mehr der mathematischen Koordinatenverbesserung zuzuwenden. Ein Polynom 2. Grades wird für jeden der 2 Bildträger eines analytischen Auswertegerätes genutzt, um die systematischen Fehler im Bereich der Variation von einzelnen Beobachtungen zu modellieren. Bezüglich der Stabilität des Instruments wird eine Anforderung formuliert. Solche Verfahren kännen auch bei anderen Geräten, wie zum Beispiel Bildscanner angewendet werden.

Notes: Photogrammetry is used extensively in British Coal Opencast to provide surface profiles of both new sites and existing sites. This paper will discuss the development of GPS within Opencast and its deployment as a tool to provide the ground control required for mapping. Comparisons with conventional equipment will also be made and relative cost effectiveness assessed. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On utilise largement la photogrammétrie dans British Coal Opencast, pour déterminer des profiles de surface, à la fois dans les sites nouveaux et dans les sites en cours. On examine dans cet article ľ emploi du GPS dans ces mines et son développement comme outil permettant ď obtenir les points ď appui sur le terrain nécessaires à la cartographie. On se propose defaire les comparaisons avec les équipements classiques et ďévaluer les avantages relatifs coût/efficacité. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die Photogrammetrie wird bei den Britischen Kohlen-Tagebauen extensiv zur Beschaffung von Oberflächenprofilen sowohl bei Neuaufschlüssen als auch bei bestehenden Gruben eingesetzt. Im Artikel wird die Entwicklung von GPS und seine Entwicklung als Hilfsmittel zur Beschaffung der für die Kartenherstellung benötigten Paβpunkte diskutiert. Es erfolgen auch Vergleiche mit der konventionellen Ausrüstung und in bezug auf die relative Kosteneffektivität.

Notes: An investigation has been made into the feasibility of using a low cost GPS receiver fitted to a light fixed wing aircraft as an aid to flying parallel flight lines for block aerial vertical photography. Two cases were examined: (I) the pilot controlled the heading of the aircraft according to the indications on the course deviation indicator of the GPS receiver; (2) the autopilot, being connected to the GPS receiver, controlled the heading of the aircraft. The results obtained for both sidelap and endlap were equal to or superior to those obtained with a CCTV system. 〈section xml:id="abs1-2"〉〈title type="main"〉RésuméOn a mené une recherche sur la faisabilité du GPS comme aide au maintien du parallélisme des axes de prises de vues d'un bloc de photographies aériennes verticales, en utilisant un récepteur GPS bon marché fié a un avion léger. On a examiné deux cas: (i) celui ou le pilote a maintenu le cap de l'avion en suivant les indications de l'index de changement de route du récepteur GPS; (ii) celui ou c'est le pilote automatique qui, par une liaison au récepteur GPS, a maintenu le cap de l'uvion. Les résultats obtenus pour le recouvrement latéral et l'extrgmité des bandes se sont révélés égaux ou supérieurs ii ceux obtenus avec un systPme CCTV.〈section xml:id="abs1-3"〉〈title type="main"〉ZusammenfassungEs wurde eine Untersuchung über die mögliche Nutzung billiger GPS-Empfanger, die in ein Kleinjlugzeug als Hilfsmittel zum Flug paralleler Bildstreifen für die blockweise Luftbildaufnahme eingebaut wurden, durchgefuhrt. Dabei wurden 2 Falle geprüft: (1) Der Pilot steuerte das Flugzeug entsprechend der Anzeige auf dem Kursindikator des GPS-Empfangers. (2) Der mit dem GPS-Empfänger verbundene Autopilot steuerte das Flugzeug. Die Ergebnisse sowohl für Querals auch Längsüberdeckung waren gleich oder besser als die mit einem CCTV-System erhaltenen.

Notes: The authors provide an account of current Directorate of Military Survey approaches to the provision of photocontrol by use of GPS and they pay special attention to the requirements of height control. 〈section xml:id="abs1-2"〉〈title type="main"〉SummaryGPS now provides us with a rapid method for determining plan and height for photogrammetric control directly over the points required by the photogrammetrist. By utilizing the slope of the geoid model, orthometric heights can be obtained to an accuracy of 0.15 m to 0.2 m. Surveys can be carried out quickly in all weather conditions and with none of the traditional terrestrial survey skills. Time, cost and manpower are greatly reduced. The plan accuracies are well within any requirements of the photogrammetrist and heights can be achieved to within 0.15 m to 0.2 m on test areas of UK. As photogrammetric height accuracy requires at best 1 m for plotting a 10 metre contour interval, heighting by GPS and the use of a geoid model solves the heighting problem.〈section xml:id="abs1-3"〉〈title type="main"〉Résumé Les auteurs fournissent un compte-rendu de la façon dont le Directorate of Military Survey aborde actuellement le problème de la fourniture de points ďappui photos avec ľemploi du GPS et prêterit une attention particulière à celui des points ďappui altimétriques.〈section xml:id="abs1-4"〉〈title type="main"〉Zusammenfassung Die Autoren geben einen Bericht über die gegenwärtigen Versuche beim Directorate of Military Survey zur Paβpunktbestimmung mit GPS, wobei sie den Anforderungen an die Höhenbestimmung besondere Aufmerksamkeit zollen.

Notes: The operational situation of precise airborne GPS positioning of camera air stations and its use in combined block adjustment for aerial triangulation is reviewed. The acceptance of systematic GPS errors, which occur especially with biased phase ambiguity solutions after loss of lock of signals in-flight, has motivated the method of additional linear GPS drift parameters which are to be solved in the combined block adjustment. It also implies that no absolute GPS co-ordinates are required and that the datum transformation is provided via a few ground control points. A great number of practical examples demonstrates that the method has been successfully applied within a wide range of photoscales and of block sizes. It has proved to be fully operational under various practical conditions. The high accuracy performance of GPS blocks and the economic benefit have been confirmed. It is particularly noticeable that the stationary receiver can be at a great distance, up to 500 km or more from the mission area. The conclusion is drawn that GPS supported aerial triangulation is ready to become standard. The potential improvement from dual frequency receivers and fast ambiguity solutions only enhances that conclusion. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On examine, dans cet article, le degréďopérationnalité atteint par le GPS aéroporté dans le positionnement précis des sommets de prises de vues des caméras aériennes, et son insertion dans la compensation par blocs en aérotriangulation. La possibilityéďerreurs systématiques dans le GPS, en particulier en cas de perte des signaux en vol, qui se manifeste par des ambiguïtés de phase et des biais dans les résultats, à conduit a introduire dans la compensation en bloc des inconnues supplémental s, traduisant une dérive linéaire du GPS. II en résulte qu'il n'est pas nécessaire de disposer de coordonnées GPS absolues et que quelques points ďappui sur le terrain suffisent à assurer les changements de coordonnées. On a utilisé avec succès cette méthode dans de nombreux essaispratiques comportant un large eventail ďéchelles de prises de vues et de tallies des blocs. On a pu ainsi mettre en évidence ľentière opérationnalité de cette méthode, dans des conditions réelles différentes. On a également confirmé la haute précision des résultats des blocs GPS et les avantages économiques de ce système. En particulier, il est tout à fait remarquable que le récepteur fixe puisse se trouver à une grande distance de la zone du lasé, 500 km ou plus. On peut en conclure que ľaérotriangulation appuyée sur le GPS est en train de devenir une méthode standard. Les améliorations possibles que peuvent apporter les récepteurs à deux fréquences et la résolution rapide des ambiguïtés, ne font que renforcer cette conclusion. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Die hochgenaue Kamera Positionierung durch GPS und ihre Anwendung für die Aerotriangulation wird zusammengefaβt dargestellt. Die Existenz systematischer GPS Fehler, wie sie inbesondere durch nur genäherte Phasen-Mehrdeutigkeitslösungen nach Signalabrissen im Flug verursacht werden, haben zu der speziellen Methode zusätzlicher linearer GPS Drift Parameter geführt, die in der kombinierten Blockausgleichung bestimmt werden. Ferner wird dabei die Datumtransformation mit Hilfe einiger weniger Paβpunkte bestimmt, d.h. es werden keine absoluten GPS Koordinaten vorausgesetzt. Mit einer groβen Zahl praktischer Beispiele wird demonstriert, daβ die Methode in einem groβen Bereich von Bildmaβstäben und Blockgröβen bereits erfolgreich angewendet wird. Sie hat sich unter den verschiedensten praktischen Bedingungen als voll operationell erwiesen. Dabei wurde die theoretisch erwartete hohe Genauigkeit der GPS Blöcke und ihre Wirtschaftlichkeit bestätigt. Besonders bedeutsam ist der empirische Nachweis, daβ der stationäre GPS Empfänger sehr weit, bis 500 km oder mehr, von dem Fluggebiet entfernt sein kann. Als Ergebnis wird empfohlen, die GPS gestützte Aerotriangulation als verfügbares photogrammetrisches Standardverfahren allgemein zu verwenden. Diese Schluβfolgerung wird durch die neuesten Entwicklungen von Zwei-Frequenz Empfängern und schnellen Mehrdeutigkeitslösungen, in die hohe Erwartungen gesetzt werden, nur noch bekräftigt.

Notes: A description is given of a heighting accuracy test carried out at Cartographical Services Limited on 1:2000 scale aerial photography. Premarked points, with ground surveyed heights, were established on a dual carriageway road near Worcester and photography was flown using a Leica RC20 aerial camera with EMC. Photogrammetric height measurement was carried out with both a Zeiss P3 analytical plotter and a Cartographic Engineering Limited/Carto Instruments AP190 plotter; respective r.m.s.e. accuracies of ± 25 mm and ± 30 mm were obtained. The new Cartographic Engineering Limited CP2 analytical plotter has subsequently been used for height measurement on similar photography and comparable accuracy has been achieved. 〈section xml:id="abs1-2"〉〈title type="main"〉Résumé On présente un essai de précision altimétrique, effectué par la Société“Cartographical Services” de Southampton, sur des photographies aérienne àľéchelledu 1:2000. On a utilisé des points pré-balisés, repartis sur une route à double voie près de Worcester, et dont ľaltitude a été déterminé sur le terrain. La prise de vue a été fait avec une caméra Leica RC20 munie ďun système de compensation du filé longitudinal. On a effectué les déterminations photogrammétriques des altitudes en utilisant à la fois un restituteur analytique Zeiss P3 et une restituteur AP190 de la Société“Cartographic Engineering/Carto Instruments.” On a obtenu comme erreurs moyennes quadratique les valeurs de ± 25 mm et ± 30 mm respectivement. On a enfin utilisé le noveau restituteur analytique CP2 de la Société“Cartographic Engineering” pour ces déterminations altimétriques et ľon a obtenu une précision comparable. 〈section xml:id="abs1-3"〉〈title type="main"〉Zusammenfassung Es wird ein Versuch zur Bestimmung der Höhengenauigkeit, der beim Cartographical Services Ltd. in Southampton für den Bildmaβstab 1:2000 durchgeführt wurde beschrieben. Signalisierte Punkte, deren Höhen im Gelände bestimmt wurden, wurden aufeiner Doppelfahrbahn in der Nähe von Worcester angeordnet undmit einerLeica RC20 mit EMC aufgenommen. Die photogrammetrische Höhenbestimmung erfolgte mit einem analytischen Auswertegerät P3 von Zeiss und einem AP 190 Plotter von Cartographic Engineering Ltd./Carto Instruments. Dabei wurden mittlere Fehler von ± 25 mm bzw. ± 30 mm erhalten. Das neue analytische Auswertegerät CP 2 von Cartographic Engineering Ltd. wurde danach für Höhenmessungen bei ähnlichen Luftbildern genutzt, wobei vergleichbare Genauigkeiten erreicht wurden.

Notes: This paper describes a method for modelling the SPOT satellite imaging process using a single Ground Control Point (GCP) and for extracting ground planimetric information. Satellite position is derived using the collinearity condition equations. Initial orbit is obtained from the given ephemeris data and refinement is carried out using an iterative least squares solution. Attitude variations are taken into account by fitting a third order polynomial in time to the attitudes derived from the ephemeris by integration. After refining the satellite position, planimetric co-ordinates can be derived using the collinearity equations with an accuracy of up to 38 m in latitude and 52 m in longitude (average of the r.m.s. errors from two tests) when the GCP used for modelling is obtained from the 1:50 000 scale map. These figures include the measurement errors as well as the modelling error. It has been found that the method is not sensitive to the location of the GCP used for modelling. Résumé On présente dans cet article une méthode de modélisation du traitement des images du satellite SPOT ne faisant appel qu'à un seul point ďappui au sol et permettant ďextraire des informations planimétriques. On obtient la position du satellite à partir des équations traduisant la condition de colinéarité. On part ďune orbite initiale issue des données fournies par les éphémerides, et on ľameliore par une résolution itèrative, basée sur les moindres carrés. On tient compte des variations ďattitude par ľintermédiaire ďun polynome du troisième ordre fonction du temps et correspondant aux attitudes dérivées des éphémérides par intégration. Une fois obtenue la position définitive du satellite, on peut tirer les coordonnees planimétriques des équations de colinéarité avec une précision qui atteint 38 m en latitude et 52 m en longitude (valeurs moyennes des erreurs moyennes quadratiques rèsultant de deux essais), lorsque le point ďappui au sol utilisé dans la modélisation provident de la carte au 1:50 000. Ces chiffres incluent les erreurs de mesure ainsi que ľerreur de modélisation. On a trouvé que la méthode nétait pas du tout sensible à la position qu'occupait le point ďappui au sol utilisé dans le modélisation. 〈section xml:id="abs1-2"〉〈title type="main"〉Zusammenfassung Im Artikel wird ein Verfahren zur Geländemodellierung des SPOT-Abbildungsprozesses unter Verwendung eines einzelnen Geländepaβpunktes (GCP) und zur Ableitung planimetrischer Informational beschrieben. Die Satellitenposition wird mit Hilfe der Kollinearitätsgleichungen beschrieben. Die Näherungswerte für die Bahnkurve werden aus gegebenen Ephemeriden bestimmt, die durch einen iterativen Prozeβ nach der Methode der kleinsten Quadrate verbessert werden. Veränderungen der Bahn werden rechtzeitig durch Anpassung mit einem Polynom 3. Grades an die aus den Ephemeriden abgeleitete Bahn durch Integration angepaβt. Nach Verbesserung der Satelliten-position können die Lagekoordinaten mit Hilfe der Kollinearitätsgleichungen mit einer Genauigkeit bis zu 38 m in der Breite und 52 m in der Länge (Mittelwert der mittleren Fehler aus 2 Versuchen) bestimmt werden, wenn der Paβpunkt (GCP) aus einer Karte 1:50 000 entnommen wurde. Dabei ist der Meβfehler ebenso wie der Modellierungsfehler enthalten. Es zeigte sich, daβ das Verfahren nicht empfindlich ist in bezug aufdie Lage des zur Modellierung benutzten Paβpunktes.

Notes: The role of the cilia in the locomotion (“gliding”) of Tetrahymena thermophila in a semi-solid medium has been studied when cells were migrating in gradients of attractant. Video recordings and computer-aided motion analysis of migrating cells and their ciliary activity show that Tetrahymena thermophila migrate by swimming forward in semi-solid methyl cellulose, using their cilia. Ciliary reversals occur at certain intervals and cause a termination (“stop”) of cellular migration. Cells with reversed cilia resume forward migration when normal ciliary beating resumes. In gradients of attractants, cells migrating towards the attractant suppress ciliary reversals, which leads to longer runs between stops than in control cells. Cells migrating away from the attractant have a higher frequency of ciliary reversals than the control cells resulting in shorter runs. Stimulated cells adapt to a particular ambient concentration of attractant several times during migration in the gradient. Adaptation is followed by de-adaptation, which occurs during the “stop”. In the presence of cycloheximide, a strong inhibitor of chemoattraction, the attractant-induced suppression of ciliary reversal is abolished (cells become desensitized to the attractant). It is concluded that Tetrahymena has a short-term memory during adaptation. This is important for the efficiency of migration towards an attractant.

Notes: Descriptive information is provided for five ciliates collected from marine coastal British Columbian surface waters. In this paper we identify three new species, Strobilidium neptuni n. sp., Strobilidium veniliae n. sp., and Strombidinopsis multiauris n. sp.; provide a new name and description for one previously described species, Strombidium siculum nom. nov. and provide more taxonomic data for Strombidinopsis cheshiri Snyder and Ohman, 1991.

Notes: Giardia lamblia trophozoites contain acetylated α-tubulin but lack detectable levels of tyrosinolated α-tubulin, as demonstrated in immunoblots with monoclonal antibodies specific for these tubulin forms. By immunofluorescence microscopy, acetylated α-tubulin is localized in axonemes, median bodies and in the adhesive disk. Post-embeddment immunogold labeling of thin sections of cells was used to evaluate acetylation at the level of individual microtubules by electron microscopy. Cells were fixed with glutaraldehyde and embedded in the acrylic resin LR Gold. Results indicate all microtubules in adhesive disk, axonemes, basal bodies, funis and the median bodies contain acetylated α-tubulin. Unlike immunofluorescence labeling, all microtubules of the adhesive disk and the funis could be gold labeled. No nonspecific labeling of the cytoplasm or of structures other than microtubules was observed. Acetylated microtubules in G. lamblia do not appear to be a subset of microtubules and acetylation appears uniform along the entire length of individual microtubules. Acetylation and the tyrosinolation state of microtubules in Giardia are discussed in the context of microtubule stability and crosslinked features of the cytoskeleton.

Notes: A model for the in vivo evaluation of antipneumocystis drugs has been developed in SCID mice infected intratracheally with cryopreserved mouse-derived Pneumocystis carinii. The development of a highly reproducible fatal P. carinii pneumonia occured within 10 weeks (mean survival time ± SEM = 72.2 ± 1.2 days). Continuous administration of dexamethasone (2 mg/liter in the drinking water) exacerbated the rate of onset of severe P. carinii pneumonia (mean survival time ± SEM = 63 ± 1.3 days) in SCID mice. The number of cysts per g of lung homogenate (homogenate counts) were maximal with an inoculum of 20,000 cysts at 6 weeks post infection. Homogenate counts correlated with infection scores (graded assessments of immunofluorescent cysts on lung impression smears) suggesting that infection scoring accurately and rapidly reflects the severity of P. carinii pneumonia in SCID mice. These studies led to the development of a drug screening protocol in which Pneumocystis-free female SCID mice (20–25 g) were started on dexamethasone 7 days prior to IT inoculation with a single dose of 20,000 cysts. Drugs were evaluated either for: a) prophylaxis (continuously from day 1 post infection) or b) treatment (from day 21 post infection) until day 42 post infection, when all mice were killed and infection scores determined. Co-trimoxazole (at 250 mg sulfamethoxazole + 50 mg trimethoprim/kg/day) given in the drinking water was found to be highly effective in both the prophylaxis and treatment of mouse P. carinii pneumonia. Co-trimoxazole remained very effective in the prophylaxis P. carinii pneumonia in the SCID mouse at 125 mg sulfamethoxazole + 25 mg trimethoprim/kg/day p.o. and showed some enhancement of efficacy over sulfamethoxazole alone at 125 mg/kg/day p.o., suggesting limited synergy between sulfamethoxazole and trimethoprim. The results presented provide confirmation of the usefulness and predictability of the model.

Notes: Immunofluorescence was used to reveal the presence of a rhodopsin-like molecule on the plasma membrane of the photoresponsive ciliate Fabrea salina. The ciliate, fixed on a glass support, showed a spontaneous red-orange fluorescence. Cells incubated with a fluoresceinated, anti-bovine opsin, sheep polyclonal antiserum showed instead a green fluorescence typical of fluorochrome excited at 436 nm, whereas cells incubated with a fluoresceinated, nonimmune sheep serum kept their original red fluorescence. Excess bovine opsin did not inhibit the antibody reaction, but detergent-coated purified bovine opsin was proven able to bind to the cell membrane. Since rhodopsin belongs to a gene-related protein family, which includes adrenergic or cholinergic receptors, the cell response to acute exposure to muscarinic and adrenergic agonist and antagonist drugs was investigated. Acetylcholine, atropine, hexamethonium, noradrenaline, or phenoxybenzamine in the culture medium (10-4 M) did not influence motion behaviour nor phototaxis of Fabrea salina. These findings raise the possibility that a rhodopsin-like molecule is present on the surface of Fabrea salina. Nevertheless, they do not prove that this surface antigen is a rhodopsin, or that this molecule is responsible for photoresponsiveness in this organism.

Notes: The life cycle of malaria parasites in the mosquito vector is completed when the sporozoites infect the salivary gland and are ready to be injected into the vertebrate host. This paper describes the fine structure of the invasive process of mosquito salivary glands by malaria parasites. Plasmodium gallinaceum sporozoites start the invasion process by attaching to and crossing the basal lamina and then penetrating the host plasma membrane of the salivary cells. The penetration process appears to involve the formation of membrane junctions. Once inside the host cells, the sporozoites are seen within vacuoles attached by their anterior end to the vacuolar membrane. Mitochondria surround, and are closely associated with, the invading sporozoites. After the disruption of the membrane vacuole, the parasites traverse the cytoplasm, attach to, and invade the secretory cavity through the apical plasma membrane of the cells. Inside the secretory cavity, sporozoites are seen again inside vacuoles. Upon escaping from these vacuoles, sporozoites are positioned in parallel arrays forming large bundles attached by multilammelar membrane junctions. Several sporozoites are seen around and inside the secretory duct. Except for the penetration of the chitinous salivary duct, our observations have morphologically characterized the entire process of sporozoite passage through the salivary gland.

Notes: CLARKE BRADBURY, Department of Zoology, North Carolina State University, Raleigh, North Carolina 27695-7617.

Notes: Hybridization using kDNA and rDNA sequences as probes was performed to study phylogenetic relatedness of different species of trypanosomatids. Using this approach, we identified five organisms which had been classified as Phytomonas and Herpetomonas that were more closely correlated to each other phylogenetically than to any other species or isolates from either genera. These findings raise doubts about the validity of the current classification of Trypanosomatidae. Finally, we demonstrated the usefulness of kDNA sequences as an alternative to genomic sequences in obtaining phylogenetic information on trypanosomatids.

Notes: The relative capacity of Na+, K+ and Cl- to stimulate germination of spores of the microsporidian Nosema algerae, a pathogen of mosquitoes, was examined by ion substitution experiments. Sodium at 0.1 M was ineffective to produce the high percentage of germination that typically occurs with 0.1 M NaCl (the normal stimulation solution) if Cl- was substituted with the usually impermeant anions SO42-, HPO42-, or the organic acids oxalate, cacodylate, EGTA, MES and HEPES. However, substantial concentration- and pH-dependent germination was seen with Na2SO4 in the 0.2-0.8 M Na+ range. Similar results were obtained with solutions of K+ accompanied by impermeant anions. In contrast, the chloride salts of usually impermeant cations, like choline and triethanolamine, failed to germinate spores even at 0.8 M unless Na+ or K+ was independently added. The presence of 0.5 M choline chloride in the medium reduced the levels of Na2SO4 required to produce germination down to equivalence with those of Na+ in the normal stimulation solution. Monensin, a Na+ ionophore, facilitated the germination induced by a medium-level stimulus (0.04 M NaCl) in sonicated samples. These findings indicate that N. algerae spores germinate in response to the alkali metal cations, while CI- plays a passive role by diffusing to maintain internal electroneutrality during cation influx. A possible mechanism of cation action in spore germination is suggested on the basis of these results and observations on other systems of intracellular motility.

Notes: Certain monoclonal antibodies interact with proteins of Tetrahymena thermophila found in the conjugation junction as well as around the gametic nuclei (pronuclei) of conjugating cells; they also react with the oral primordium and fission zone of vegetative cells and with the cytoproct and contractile vacuole pores of all cells. One of these (FXIX-3A7) was investigated in detail. Immunogold labelling suggests that the material labelled by the 3A7 monoclonal antibody, which we call “fenestrin,” is located beneath the epiplasm (membrane skeleton). Immunoblots reveal that the major and perhaps sole antigen is a 64 kDa polypeptide, found in two isoelectric variants. Developmental studies implicate fenestrin in two processes involved in conjugation. The first is “tip transformation.” During preliminary starvation (“initiation”), labelling of fenestrin first appeared as a spot at the anterior end of starved mature cells, then after mixing of different mating types (“costimulation”) it extended posteriorly along the anterior suture. After pairing, this region spread to form a widened plate. The second process is pronuclear transfer. Fenestrations representing channels between the conjugating cells began to appear 0.5 to 1 h after the conjugants united, and eventually merged to form a small number of temporary large holes during exchange of the transfer pronuclei. A fenestrin envelope also enclosed both the transfer and resident pronuclei; a strand of fenestrin connected the two. Shortly after pronuclear transfer, both transfer and resident pronuclei were released from fenestrin caps and fused to produce a zygotic nucleus (synkaryon) not associated with fenestrin. Fenestrin thus appears to be intimately involved in the process of pronuclear exchange.

Notes: . The ultrastructure of meiospores of six different species of Amblyospora found infecting larval mosquitoes of Aedes abserratus, Aedes aurifer, Aedes cinereus, Aedes excruciates, Aedes sticticus, and Aedes stimulans are described. Meiospores of all species exhibited characteristics typical for the genus Amblyospora including: a single nucleus, a large posterior vacuole, a thick undulating exospore and thinner endospore, a bipartite and lamellate polaroplast with more tightly stacked membranes in the proximal region, and an anisofilar polar filament with distal coils reduced in thickness. Distinct differences were found in the arrangement and number/ ratio of coils formed by the broad basal and narrow distal portions of the polar filament. These differences, when quantified and averaged, were unique from all other mosquito-parasitic species that have been examined ultrastructurally. Information on parasite development, natural field prevalence and transmission to suspected intermediate copepod hosts is presented. The creation of six new species, Amblyospora abserrati, Amblyospora auriferi, Amblyospora cinerei, Amblyospora excrucii, Amblyospora stictici, and Amblyospora stimuli is proposed. Synonymies and complete host lists of all forms and species of Amblyospora described from mosquitoes are given.

Notes: . The α- and the β-tubulin genes of the hypotrichous ciliate Euplotes octocarinatus were isolated from a size-selected macronuclear DNA library. The α-tubulin gene is located on a 1,587 bp macronuclear DNA molecule and the β-tubulin gene on a 1,524 bp macronuclear DNA molecule. Sequencing revealed that all the cysteine residues of the two genes are encoded by the common cysteine codons UGU and UGC and none by an UGA codon. This is in contrast to the genes of E. octocarinatus sequenced so far, where some of the cysteines are encoded by the opal codon UGA. The tubulin genes end like other Euplotes genes with a TAA. They do not contain introns. The last codon for an amino acid in the α-tubulin gene is a GAA which codes for glutamic acid. This is in contrast to what has been reported for most α-tubulin genes, but it supports findings for other hypotrichous ciliates. No evidence for the existence of more than one type of α- and one type of β-tubulin genes could be obtained.

Notes: Intrachromosomal variant surface glycoprotein (VSG) genes in Trypanosoma brucei are expressed by a mechanism involving gene conversion. The 3’boundary of gene conversion is usually within the last 130 bp of the VSG gene, a region of partially conserved sequences. We report here the loss of the predominant telomeric A VSG gene in the cloned variant antigenic type (VAT) 5A3, leaving only an intrachromosomal A VSG gene (the A-B gene). The nucleotide sequence of the A-B VSG gene reveals that it lacks the normal VSG 3′ sequence. Surprisingly, we find cells expressing this A-B VSG gene in relapse populations arising from VAT 5A3. Since the A VSG mRNAs from these cells have a normal 3′ sequence, the incomplete A-B VSG gene must be expressed via a partial gene conversion that supplies the functional 3’end. Although the A-B VSG gene is no longer predominant like the telomeric A VSG gene, it is still expressed more frequently than other intrachromosomal VSG genes, suggesting that factors other than a telomeric location determine whether a VSG gene is expressed early in a serodeme.

Notes: Mixed life cycle stages of rat-derived Pneumocystis carinii were isolated from host lungs and their sterols were compared with those present in lungs from normal and immunosuppressed uninfected rats. Gas-liquid chromatography consistently detected, resolved, and quantified 9, 10, and 20 sterol components in the total nonsaponifiable neutral lipid fraction of lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii preparations, respectively. In all samples, cholesterol was the most abundant sterol present, comprising 97%, 93%, and 78% of total sterols in lungs from normal rats, lungs from immunosuppressed uninfected rats, and P. carinii, respectively. Tentative identifications of several rat lung and P. carinii minor sterols were made based on gas-liquid chromatogram retention times and fragmentation patterns from mass spectral analyses. Campesterol (ergost-5-en-3-ol), cholest-5-en-3-one, and β-sitosterol (stigmast-5-en-3-ol) were among the minor components present in both types of lung controls, and were also components of P. carinii sterols. In contrast to lung controls, the sterols of P. carinii were enriched in C28 and C29 sterols with one or two double bonds, and a hydroxyl group at C-3 (ergost-5-en-3-ol, ergost-7-en-3-ol, ergosta-dien-3-ol, stigmast-5-en-3-ol, stigmast-7-en-3-ol and stigmasta-dien-3-ol). Steryl esters of P. carinii, probably stored in cytoplasmic lipid droplets, were dominated by those present in the host lung. In separate studies. 3-hydroxy-3-methylglutaryl coenzyme A activity, a key enzyme in the regulation of sterol biosynthesis, was detected in purified P. carinii preparations and incorporation of radiolabeled squalene and mevalonate was observed. Together, these results suggest that the parasite readily takes up and incorporates host sterols, and that the organism synthesizes some of its own “metabolic sterols”

Notes: Cultured P. carinii rapidly took up a variety of fatty acids. The relative rates of uptake for four fatty acids were 18:1 〉〉 16:0 ≅ 18:0 ≅ 18:2. Fatty acids were primarily incorporated into phospholipids and the uptake process was specifically inhibited by 2.2 and 22 μM primaquine, a concentration having no effect on host cells. Amino acids were also taken up by cultured P. carinii in a primaquine sensitive process. Radiolabeled leucine was incorporated into the major surface glycoprotein of P. carinii. The formation of radioactive P. carinii-specific proteins indicated that the cultured organisms carried out transcription and translation and that the incorporation of amino acids was dependent upon P. carinii rather than rare HEL human embryonic lung cells. The spinner flask culture system provides convenient access to P. carinii for metabolic studies in defined medium for a period of 5–14 days after inoculation.

Notes: The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA-/myoB-, myoB-/myoC-, and myoA-/myoC-) were examined with respect to their cytoskeletal organization. F-actin localization by rhodamine-phalloidin staining of cells indicates that the myoA-/myoB-, myoB-/myoC-, and myoA-/myoC- cells appear to redistribute their F-actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.

Notes: The new microsporidium, Napamichum cellatum, a parasite of the adipose tissue of midge larva of the genus Endochironomus in Sweden, is described based on light microscopic and ultrastructural characteristics. Plurinucleate Plasmodia with nuclei arranged as diplokarya divide, probably by plasmotomy, producing a small number of diplokaryotic merozoites. The number of merogonial cycles is unknown. Each diplokaryotic sporont yields eight monokaryotic sporoblasts in a thin-walled, more or less fusiform sporophorous vesicle. A small number of multisporoblastic sporophorous vesicles were observed, in which a part of the sporoblasts were anomalous. The sporogony probably begins with a meiotic division. The mature spores are slightly pyriform. Fixed and stained spores measure 2.1-2.4 × 3.7-4.5 μm. The five-layered spore wall is of the Napamichum type. The polar filament is anisofilar with seven to eight coils (142-156 and 120 nm wide). The angle of tilt is 55-65°. The polaroplast has an anterior lamellar and a posterior tubular part. The granular, tubular and crystal-like inclusions of the episporontal space disappear more or less completely when the spores mature. The crystal-like inclusions are prominent in haematoxylin staining, but not visible with the Giemsa technique. The microsporidium is compared to other octosporoblastic microsporidia of midge larva and to the species of the genera Chapmanium and Napamichum.

Notes: CB1-glycoprotein is a component of flagellar pocket, endosome, and lysosome membranes of long, slender bloodstream forms of the Trypanosoma brucei subgroup of African trypanosomes. We have used immunoblotting, immunofluorescence, and cryoimmunoelectron microscopy to study CB1-glycoprotein expression as long, slender bloodstream forms of pleomorphic T. b. brucei and T. b. gambiense transform through intermediate stages into short, stumpy forms. Intermediate and stumpy forms express more CB1-glycoprotein than long, slender forms. These results, coupled with previous work showing that procyclic forms do not express CB1-glycoprotein, show that the expression of lysosomal membrane glycoproteins is regulated coordinately with other aspects of lysosome and endosome function as these trypanosomes go through their life cycle.

Notes: Trichocyst discharge is an effective defense of Paramecium against Dileptus margaritifer. The possible defensive function of backward swimming, which often follows trichocyst discharge upon Paramecium-Dileptus encounters was studied. Mutants incapable of backward swimming (pawnA in P. tetraurelia, cnrA in P. caudatum) escaped from dilepti nearly as frequently as wild-type cells. Double mutants (pawnA-nd7, cnrA-tnd2) were eaten nearly as frequently as mutants incapable of trichocyst discharge. Thus, in the defense of Paramecium against D. margaritifer, the role of backward swimming is minor, if any, compared to trichocyst discharge. Among escaped cells, about a half of wild-type and essentially none of pawnA (cnrA) cells showed backward swimming. Paramecium behavior during the encounter can be mimicked by the local, not global, application of lysozyme which is a strong secretagogue of trichocyst.

Notes: A total of 4.1% infestation with Chilodonella spp. was found among fish studied in 144 tanks in 1987–1989, representing 14.0% of the tanks in which fish are reared at four salmonid farms in northern Finland. Two species were found, C. hexasticha and C. piscicola, and both occurred on salmon (Salmo salar L.), sea trout [S. trutta m. trutta (L.)] and brown trout [S. t. m. lacustris (L.)]. Variability was observed in the length and width of the C. piscicola specimens and the number of ciliary rows or kineties. Large specimens which had more kinetics than average for C. piscicola were found mainly on the skin of salmon aged 1–2 years. The number of kinetics in the right ciliary band was found in stepwise logistic regression analysis to be of importance when typing C. piscicola specimens. Fingerlings were found to be more susceptible to Chilodonella infestation than older fish, and mortality varied in the range 2–10% in the course of the epizootics in the three fish species. Most mortality cases were caused by C. hexasticha, occurring mainly on the gills of the fish. Chilodonella piscicola was most often found in salmon and occurred at lower water temperatures than C. hexasticha (mean water temperature when found for the first time being 13° C and 16° C, respectively).

Notes: Chlamydomonas reinhardtii exhibits photophobic and positive and negative phototactic responses that can be defined for cell populations using computerized cell tracking and motion analysis. Mutants CC-2359 and FN68 are pigment deficient mutants that are blocked in carotenoid synthesis and lack these photo responses. In particular, neither mutant exhibits flash-induced photophobic responses to visible light stimuli to which wild-type gametic cells exhibit a strong response, with several behavioral stages. Upon addition of all-trans retinal to these mutants, the photophobic responses are restored with minor quantitative differences from wild-type populations. Using both light and electron microscopy, we have compared the ultrastructural characteristics of wild-type C. reinhardtii to those of both mutants. As previously described, wild-type cells contain an eyespot consisting of 2–4 layers of pigmented granules encased within thylakoid membranes, located between the distal extremities of the flagellar root. This structure is also visible as an orange-red spot in light microscopy. The photoreceptor is thought to be concentrated in the plasma membrane above the eyespot. The mutant, CC-2359, lacks this eyespot as seen by both light and electron microscopy, even when the photophobic response has been restored. FN68-like mutants studied earlier by Morel-Laurens and Feinlieb and others contain an eyespot which can be seen only by electron microscopy. In FN-68, the eyespot generally has the same dimensions as in wt cells, differing mainly in pigment granule appearance. Consistent with these findings, several laboratories have shown that the full range of phototactic responses can be reconstituted in FN68 and CC-2359, but that negative phototaxis requires a significantly stronger light stimulus in the latter strain. We confirm the suggestion that the eyespot is not necessary for the photophobic response, and is not critical for the appropriate assembly and function of the photophobic response receptor in the membrane. Furthermore, the locus of reconstitution of the functional receptor is not the eyespot. Because of the definitive demonstration of the absence of the eyespot in CC-2359, however, the eyespot may play a role in negative phototaxis.

Notes: The rDNA repeat unit from a new human Giardia duodenalis strain shows significant differences from the previously reported G. duodenalis rDNA repeat. Twelve base-pair changes occurred in 490 bp of the SSrRNA gene and new restriction enzyme sites occurred in the LSrRNA gene. The overall length of the rRNA genes is the same but the spacer is 76 bp longer than previously reported. A boundary within the spacers of the two different rDNA units divides a region of 50% homology near the LSrRNA gene from a region of 80% homology toward the SSrRNA gene. This boundary region includes two copies of a 78 bp repeat.

Notes: Twenty-one different strains of Euplotes of the cirrotype 10, double dargyrome morphotype were collected in marine and brackish habitats from widely varying geographic locations. The strains were cultured under similar conditions prior to fixation and staining. The morphological features considered included the cell lengths and widths, the number and position of all the ventral cirri, adoral membranelles and dorsal cilia, and the position of the peristomal shelf. Univariate and multivariate analyses revealed continua in these measurements among the strains, as well as significant correlations between cell length and the number of adoral membranelles, the number of dorsal cilia, and the length, width and area of the oral cavity. Based on data from this study and from previous studies, we conclude that the traditional criteria used for species descriptions within this morphotype are insufficient to provide robust distinctions. Therefore, we submerge all of the descriptions within this morphotype under the nomenclaturally superior Euplotes charon Müller, 1786.

Notes: The Hartmannella vermiformis small-subunit rRNA coding region was amplified, and the amplified DNA was cloned and sequenced. The coding region is 1,840 nucleotides long, and is typical of eukaryotic rRNA genes in both size and composition. Different clones contained different nucleotides at three positions.

Notes: The micronuclear version of the gene encoding β-telomere binding protein (β-TBP) in Oxytricha nova has been sequenced and compared to the macronuclear β-TBP gene, previously described. The micronuclear gene contains three AT-rich internal eliminated sequences (IES) of 37, 40, and 43 bp and four macronuclear destined sequences (MDS). The IES interrupt the gene once near the 5′ end of the coding region and twice in the 3′ trailer downstream from the TGA stop codon. The sequences of the micronuclear and macronuclear genes are colinear. Thus, the micronuclear β-TBP gene is not scrambled, which contrasts with the highly scrambled state among the 14 MDS in the micronuclear α;-TBP gene.

Notes: A new species of marine interstitial ciliate, Tracheloraphis primitarum sp. n., is described from intertidal sands (160-200-μm medium grain size) along the Northwest Atlantic coast. Living specimens are not transparent under incident light, 0.4-0.9 mm in length, and are characterized by an elongated body, bulbous anterior and rounded posterior regions. Cytostome is simple without a cleft on the lip. Protargol silver impregnation shows 18-29 longitudinal kineties. The glabrous zone is very narrow, 6-7 μm wide, and corresponds to the area occupied by one kinety and two interkinetal spaces. Ten to fifteen kineties terminate against the glabrous zone. Interkinetal extrusomes are present. The nuclear apparatus is a single centrally-placed nuclear group that consists of three large irregular macronuclei and 2-3 micronuclei; these are either clustered or encapsulated.

Notes: The most commonly encountered protostelids are nominal members of the genus Protostelium Olive & Stoianovitch. These are Protostelium mycophaga Olive & Stoianovitch and P. irregularis Olive & Stoianovitch. Both species share the common features of long-stalked fruiting bodies with single, uninucleate, deciduous spores and trophic states that consist solely of uninucleate amoebae, but they are quite different with respect to their detailed structure at both the light and electron microscopic levels. Based on this evidence, it seems unlikely that the species are congeneric, and it is proposed that P. irregularis be assigned to Soliformovum n. g. Examination of other species of Protostelium indicates that P. nocturnum Spiegel should be retained in the genus and that P. expulsum Olive & Stoianovitch should be reassigned to Soliformovum. The group most closely related to Protostelium, under this new concept, is the genus Planoprotostelium Olive & Stoianovitch, while Soliformovum is best treated as a genus of Eumycetozoa incertae sedis, though it does share some similarities with the group that includes the genera Ceratiomyxella Olive & Stoianovitch, Nematostelium Olive & Stoianovitch, and Schizoplasmodium Olive & Stoianovitch.

Notes: Dyer, B. D. & Obar, R. A. 1994. Tracing the History of Eukaryotic Cells. The Enigmatic Smile. Dyer, B. D. & Obar, R. A. 1994. Tracing the History of Eukaryotic Cells. The Enigmatic Smile. Plattner, H. 1993. Advances in Cell and Molecular Biology of Membranes.

Notes: A monoclonal antibody (mAb) IR-2-1 was raised against a 67-kDa protein purified from the macronucleus-specific bacterial symbiont Holospora obtusa of Paramecium caudatum. The mAb was found to react with two bands (31 and 67-kDa) on gels of H. obtusa. Indirect immunofluorescence microscopy showed that these antigens were distributed inside the cells. However, unexpectedly, this mAb also cross reacted with the radial arms of the contractile vacuole in P. caudatum, P. tetraurelia, P. multimicronucleatum, P. jenningsi and P. bursaria as well as with their cytoplasm. Immunoelectron microscopy showed that the antigens were located on the decorated spongiome of the radial arms. In immunoblots, mAb IR-2-1 reacted with a band of 67 kDa in all Paramecium species examined. However, no band appeared in the immunoblot of isolated macronuclei of H. obtusa-free P. caudatum and no label was seen in the nuclear matrix of the macronucleus of air-dried P. caudatum. These results suggest that the 67-kDa antigen found in H. obtusa was not imported from the host macronucleus and the same antigen in the host contractile vacuoles and cytoplasm were not derived from the symbiont. These results also showed that an epitope on the decorated spongiome of the Paramecium species is shared by its bacterial symbiont. In contrast to the decorated tubule-specific mAb, DS-1, the antigens for IR-2-1 appeared to be loosely membrane bound as they were lost in paraformaldehyde fixed and acetone permeabilized Paramecium. Supplementary key words. Contractile vacuole complexes, Holospora obtusa, monoclonal antibody, Paramecium.

Notes: By adding the protein synthesis inhibitor, emetine (10-4 M) to a highly synchronized population of Crypthecodinium cohnii Biecheler 1938 at different phases of its cycle, we were able to determine: 1. The existence and the lengthening of the G2-Phase (30 min) in the first cycle (cycle with swimming G1 phase). 2. The time of the second cell cycle phases (cycle in the cyst): G1, 30 min; S, 1.5 h; G2, 2 h and M, 2 h. These results, together with the estimation of the cell volume of the two and four swimming daughter cells emerging from the cysts, allowed us to state the existence of two transition points: G1/S and G2/M, which are necessary for completion of mitosis. We completed this refined approach of the cell cycle in studying the activities of the histone H1 kinase either in dividing or in non-dividing Crypthecodinium cohnii cells with either total soluble proteins or the isolated mitotic kinase complex. The H1 kinase activity of this purified complex is noticeably higher (twice as high) in the dividing cells than in the non-dividing ones. These data are discussed in the light of the basic characteristics of the dinokaryon, and also compared with recent biochemical observations on the same organism and studies on other higher eukaryotic protists and metazoa.

Notes: . Classical genetic techniques were applied to clonal cultures of the Euplotes vannus-crassus-minuta sibling species complex in an effort to provide some resolution to the species problem among these hypotrichs. Complex mating interactions were observed among clonal stock cultures derived from samples collected from sympatric and allopatric populations in a wide geographic survey. These results suggested that the classical model for the mating type inheritance and determination in these Euplotes is necessary but not sufficient to describe the mating mating interactions among populations of these ciliates. Successful conjugation between the nominal species E. crassus and E. vannus was observed routinely, and crosses between these two nominal species did not differ significantly from those among the other clonal stock cultures with respect to mating intensity and exconjugant survival. Data from backcrosses suggests that E. vannus and E. crassus can and do exchange genes. Based upon these data, we conclude that E. vannus and E. crassus comprise a single, highly polymorphic species with countless small populations, among which incomplete genetic exchange takes place.

Notes: . Twenty different clonal strains of marine and brackish Euplotes, representing four morphotypes, were tested for hyposalinity tolerance by a method which gradually acclimated the cells to lower salinity medium. The lowest salinities in which the strains could thrive ranged from 60% of normal seawater to complete freshwater. The morphological effects of culture medium salinity were also examined for two strains of a small “Euplotes charon” morphotype, as well as for two mating compatible “Euplotes vannus” strains and several of their exconjugates. There were no differences between the euryhaline strains grown in fresh or saltwater, except for a slight increase in overall cell size in one strain when cultured in freshwater medium. E. vannus strains increased in overall cell size with decreased salinity; also, the dorsal surface of the cells can become disorganized when the cells are cultured in 30% normal seawater.

Notes: . A method for discriminating among Leishmania is described, based upon small subunit ribosomal DNA sequence differences. The method was to amplify the entire 2.2 kb small subunit rDNA by polymerase chain reaction using conserved primers specific for the 5′ and 3′ termini of the small subunit ribosomal RNA, and then hybridize the product dotted onto nylon membranes with labeled oligonucleotides. The design of the hybridization probes was based upon complete small subunit rDNA sequences from L. amazonensis, L. major and L. guyanensis and partial sequences of L. mexicana, L. braziliensis, L. tropica and L. chagasi. A high degree of sequence similarity (〉 99%) among species was found. However, sufficient sequence divergence occurred to permit the design of internal oligonucleotide probes specific for species complexes. This procedure successfully discriminated amongst a wide range of Leishmania isolates. The method detected as few as 10 cultured organisms and detected parasites in tissue samples from experimentally infected animals. Non-radioactive labeling showed the same specificity and sensitivity as radioactive probes.

Notes: . Highly-pathogenic, mouse-passaged Naegleria fowleri amoebae are complement resistant. The present study evaluates the effect of complement on N. fowleri and the virulence of the amoebae after animal passage and growth in two different axenic media. Pathogenic N. fowleri maintained in “enriched” Cline medium are virulent for mice and resistant to complement lysis. A rapid decline in resistance to complement and virulence for mice is observed when highly-pathogenic N. fowleri are grown in Nelson medium lacking hemin. N. fowleri maintained in Nelson medium can be rendered complement-resistant by shifting the amoebae to growth in Cline medium for 2 h prior to the addition of complement. Cycloheximide treatment of N. fowleri maintained in Nelson medium blocks the transition to a complement-resistant phenotype following a shift in growth medium. Proteins were radiolabeled with [35S] during a shift from Nelson to Cline medium to identify specific polypeptides which may be associated with the functional activities related to virulence and resistance to complement.

Notes: . Large percentages of Toxoplasma gondii tachyzoites could be induced to display two types of movement associated with active invasive behavior by exposing them for 1 min to 0.002% trypsin in phosphate-buffered saline (PBS). The motile activity, consisting of clockwise rotation around the posterior end (about 20 revolutions per min) and twirling-gliding over a poly-L-lysine substrate (1.2 ± 0.2 μm/s standard deviation), was observed and recorded by video-enhanced contrast microscopy. The number of active tachyzoites reached a maximum 1 min after trypsinization; the motile response of the population lasted for about 5 min. Activation was prevented by soybean trypsin-inhibitor, and could not be induced again in previously treated specimens. Electron-microscopy of trypsinized tachyzoites fixed in the presence of ruthenium-red revealed discrete discontinuities of the plasma membrane, which sealed within 90 min after washing with PBS. Treated tachyzoites were able to invade cultured epithelial cells with a higher relative infectivity than that of untreated parasites. Perfusion of trypsinized tachyzoites with 1 mM of either CaCl2 or MgCl2 and 1 mM ATP increased the number of activated parasites to over 60%; on the other hand, all induced motility was inhibited or blocked by agents that chelate divalent cations. The present preparation, which provided the first serial illustrations of T. gondii movements induced by a defined chemical stimulus, may offer a useful experimental model for the study of motility in this parasite.

Notes: Lagenophrys novazealandae n. sp. occurs on the gills of Paranephrops zealandicus, a parastacid crayfish from New Zealand. The new species has the hemispheroidal lorica most common among members of its genus and is distinguished by its possession of large tubercles on the thickened edge of the anterior lip of the lorica aperture, a deep cleft in the left side of the lip's edge, and a ovoid to reniform macronucleus located in the right-hand part of the body. It is probable that an as yet unnamed species of Lagenophrys known to occur on another species of Paranephrops in New Zealand is distinct from L. novazealandae but phylogenetically related to it. Lagenophrys petila n. sp. occurs on setae of Parastacoides tasmanicus, a parastacid from Tasmania. The new species has an ovoid lorica tapering to a slender pseudostalk at the posterior end, a type of lorica possessed by only two other members of its genus that also attach to their host's setae. It is distinguished from the other ovoid species by the proportions of the lorica, the extreme shortness of the lips of the lorica aperture, and an ovoid macronucleus located in the right, anterior part of the body. Clefts in the lips of L. novazealandae and other members of Lagenophrys may function as points of flexure to allow the lips to bend in ways that accommodate interspecific differences in the size of the epistomial disk and its operation during suspension feeding.

Notes: . Parasitic amebas propagate among hosts through cysts, the resistant forms in their life cycle. In spite of their key role in infection, little is known about the encystation process and the mechanisms involved in reaching this stage. Two features drastically affected by encystation are motility and cell shape, both of which are determined by the cytoskeleton, composed mainly of actin in these organisms. Therefore, we studied the occurrence and relative levels of actin and actin synthesis during encystation of Entamoeba invadens. Using a cDNA actin probe obtained from a library of E. histolytica and a monoclonal antibody against actin, we found that, while the total actin levels sharply decrease as encystation proceeds, the levels of actin mRNA are reduced only in mature cysts. Moreover, actin synthesis does not take place in precysts and the later stages of cyst formation. In contrast, the levels of other proteins remain stable in trophozoites, precysts and cysts, and stage specific peptides are actively synthesized in precysts. The results indicate that encystation is accompanied by a preferential down-regulation of actin synthesis and a decrease in actin levels. The reorganization of the cytoskeleton occurring as trophozoites transform into round, quiescent cells, could be a regulatory factor in the observed changes.

Notes: . Metanophrys diminuta n. sp., a marine philasterine scuticociliate collected from the south Florida coast, was preserved for transmission electron microscopy using a mixed fixation technique. Rough endoplasmic reticulum profiles were found proximal to mitochondria and within mitochondrial fenestrations (or deep pockets). In longitudinal sections mitochondrial profiles appear to be very long, occupying almost the entire length of the cell. The micronucleus is roughly spherical to ovoid, semi-enclosed in the macronucleus. The oral polykinetid #1 is composed of two files of kinetosomes. The c segment of the oral dikinetid is composed of three to four kinetosomes. This species is the smallest within the genus Metanophrys.

Notes: . The microsporidium Nadelspora canceri n. g., n. sp., is described from the striated musculature of the Dungeness crab (Cancer magister) in Oregon, USA. The needle-shaped spores were rounded anteriorly, tapered to a posterior point and measured 7.1–11.8 × 0.2–0.3 μm in fixed preparations. The extremely narrow spore diameter prevented observation of morphological details at the light microscopic level and ultrastructural details of mature spores were difficult to resolve. Meronts were not observed and the monokaryotic merozoites and sporonts were not contained within either parasitophorous or sporophorous vesicles. Sporonts were disporoblastic and gave rise to monokaryotic sporoblasts that became narrow and elongate as they developed into immature spores with a developing polar filament. The nucleus was not clearly resolved in mature spores and may have been surrounded by the lamellar polaroplast. The polar filament was of nearly uniform diameter throughout most of its length and ended abruptly about three-fourths of the distance from the anterior end of the spore. Unusual spherical non-membrane bound granules surrounded the polar filament in a spiral arrangement. The new microsporidium resembles members of the family Mrazekiidiae, but differs in lacking a diplokaryon at any stage. It is probably most closely related to Baculea daphniae from which it differs primarily by spore shape and size. The familial relationships of the genus Baculea have not been determined and it is proposed to include it with Nadelspora in the new family Nadelsporidae.

Notes: . The clonal life history of ciliated protists is characterized by a sequence of phenotypes; sexual immaturity, maturity, and senescence. The distinctiveness of immaturity and maturity has been investigated. Standard assays of the onset of maturity of progeny clones from a cross between stocks EC1 and EC2 of Euplotes crassus demonstrated significant differences among clones and among testers within clones. They also revealed that the first positive test(s) of a progeny subclone were typically followed by at least one negative test. Special protocols were devised to investigate if maturity was reversible at the cellular level. In these experiments, the first mating pair of a progeny subclone was split before the consummation of mating. From these two cells as well as from control progeny and tester cells, subclones were established and every leftover cell was tested for maturity after each transfer. Both standard and split-pair progeny subclones had immature and slow- to-mate cells. The number of fissions before progeny exhibited sexual behavior indistinguishable from the testers was more than twice that to the first mating reaction of a subclone. At the first sign of maturity, progeny lines are a heterogeneous population of cells able and not able to mate, but remarkably, clonal descendants of those able to mate may become unable to mate. The development of maturity is progressive, quantitative and non-monotonic rather than an instantaneous switch.

Notes: . Cell division in higher eukaryotes is mainly controlled by p34cdc2 or related kinases and by other components of these kinase complexes. We present evidence that cdc2-like kinases also occur in Paramecium. Two polypeptides reacted with an antibody directed against the perfectly conserved PSTAIR region found in cdc2 kinases in other eukaryotes. Only the less abundant peptide bound to p13suc1 from Schizosaccharomyces pombe. Using centrifugal elutriation to select cells on the basis of size, we isolated highly synchronous Paramecium G1 cells. With this procedure, we demonstrated that the p13suc1-associated cdc2-like histone H1 kinase was activated before cell division at the point of commitment to division in Paramecium. Further, we show that Paramecium cdc2-like proteins occurred principally as monomers and that these monomers were active as histone H1 kinases in vitro.

Notes: . Strombidium purpureum Kahl, is an anaerobic oligotrichous ciliate with endosymbiotic phototrophic bacteria. Like other anaerobic ciliates, S. purpureum reacts to O2-pressure. In the light, the ciliates avoid even traces of O2 (〈 1% atmospheric saturation). In the dark, however, the ciliates accumulate in water with a pO2 of 1–4% atmospheric saturation. Experiments show that ciliates which have accumulated in the dark under microaerophilic conditions react when the light is turned on, and their ability to escape is enhanced by steep O2-gradients. The ciliates orient themselves in O2-gradients by a series of phobic responses. They tumble whenever they swim towards a higher O2-tension and thus they eventually all swim in the direction of lower pO2. The ecological implications of such behaviour are discussed.

Notes: . The application of an immunocytochemical method to identify precystic stages and to analyse the encystment kinetics, by using a polyclonal antiserum against isolated cyst walls from the ciliate Colpoda inflata, is reported for the first time. Three different precystic phases were chosen on the basis of morphological changes and degree of cyst wall formation. By using this procedure a better identification of mature resting cysts with regard to precystic cells or young cysts is provided. An average consensus encystment kinetics of C. inflata, by using an accumulated class frequency analysis, is reported.

Notes: . Using RNA: RNA in situ hybridization, the intracellular location of a transcript encoded by and spanning the entire length of a Trypanosoma cruzi kinetoplast DNA minicircle was determined. In axenically cultured T. cruzi epimastigotes, the hybridization signal was restricted to the kinetoplast, which was situated in the perinuclear region of the cell. Following conversion of epimastigotes to culture-derived metacyclic trypomastigotes, the kinetoplast moved to an acentric position in the metacyclic trypomastigote. Again, the hybridization signal co-localized with the position of the kinetoplast. These results suggested that the transcript remained closely associated with the T. cruzi kinetoplast within the mitochondrion in each of the morphological forms. Using specific oligonucleotide probes derived from a cDNA encoding the transcript, the entire native kDNA minicircle encoding the transcript was cloned and its nucleotide sequence was determined. The nucleotide sequence of the intact native minicircle was identical to that of the full-length cDNA corresponding to the minicircle transcript, indicating that the transcript was not modified prior to the time of cDNA synthesis and cloning.

Notes: . Eukaryotic mitotic cell cycles have been extensively studied in yeasts and vertebrate cells but little is known about cell cycle mechanisms in early branches of the eukaryotic lineage. Trichomonas vaginalis represents one of the earliest branching eukaryotic lineages available for study. In contrast with most yeasts and vertebrate cells, the T. vaginalis G2 period was prolonged, comprising 50 to 58% of the cell population. Hydroxyurea, aphidicolin, and excess thymidine, all of which arrest yeasts and vertebrate cells at the G1/S phase boundary, had no effect on the T. vaginalis cell cycle, probably due to the known absence of synthetic pathways. The antimicrotubule mitotic inhibitors, colchicine and nocodazole, induced G2 phase synchrony. Metronidazole, a therapeutic reagent, also caused G2 phase arrest. These observations suggest that T. vaginalis is similar to yeasts and vertebrate cells in G2 and M phases, but the parasite's G1/S phase transition is distinctive. The results also suggest potentially therapeutic, anti-trichomonad activity of microtubule inhibitors such as nocodazole. The cultured parasite may prove useful as a model for the mitotic cell cycle in the absence of G1/S phase transitional activities universal in yeasts and vertebrate cells.

Notes: . Reversible changes in kinetoplast DNA (kDNA) minicircles sequences were observed in clones of Trypanosoma cruzi strain Y, following a number of passages during exponential growth phase or after subcloning in blood-free medium. kDNA restriction patterns of clones were similar to those of the original uncloned strain, while subclones presented distinct kDNA restriction patterns. Homology experiments demonstrated strong hybridization between kDNA with the same electrophoretic mobility patterns while only weak signals were observed with kDNA of different patterns. The changes observed, which are unprecedented in T. cruzi clones, characterize transkinetoplastidy, and seem to be associated with similarly reversible changes both in zymodeme and in infectivity.

Notes: . Growth of Acanthamoeba castellanii in batch culture at 30° C was associated with marked changes in cellular fatty acid composition. The largest change occurred in the linoleate to oleate ratio, which was maximal in early- to mid-exponential phase cultures but decreased approximately 10-fold as cells approached stationary phase. The higher degree of lipid unsaturation in young cultures was accentuated by a greater proportion of 20-carbon polyunsaturated fatty acids than in stationary phase cultures. The unsaturation index (average number of double bonds per fatty acid) was maximal in mid-exponential phase cultures after 24 hours growth. Incorporation of [1-14C]acetate into polyunsaturated fatty acids in short-term (2 hour) experiments was high in 12 and 24 hour old cultures, where linoleate and eicosadienoate accounted for up to 26% of total labelled fatty acids. Incorporation of [1-14CJacetate into these fatty acids was negligible in stationary phase cultures. These results were correlated with changes in the specific activity of the Δ12-desaturase. Δ12-Desaturase activity was greatest in microsomal membranes isolated from early- to mid-exponential phase cells, but declined by approximately 50% as cultures progressed towards stationary phase. Membrane fractionation studies revealed that although some differences in fatty acid composition between plasma-membrane, mitochondrial (enriched), and microsomal membrane fractions were evident, the large changes in lipid unsaturation in whole cells of A. castellanii could not be accounted for by differential development of particular subcellular membranes.

Notes: Avila, J. L. & Harris, J. R. (ed.). 1992. Subcellular Biochemistry, Volume 18: Intracellular Parasites. Plenum Press, New York.Fensome, R. A., Taylor, F. J. R., Norris, G., Sarjeant, W. A. S., Wharton, D. I. & Williams, G. L. 1993. A Classification of Living and Fossil Dinoflagellates. Micropaleontology Special Publication No. 7. Micropaleontology Press, American Museum of Natural History, New York.Volberding, P. & Jacobson, M. A. (ed.). 1993. AIDS Clinical Review 1993/1994. Marcel Decker, Inc., New York.Hyde, J. E. (ed.). 1993. Protocols in Molecular Parasitology, Volume 21 in the Methods in Molecular Biology Series. Hurnana Press, Totowa, New Jersey.

Notes: . Four different tubulin genes were identified in the somatic nucleus (macronucleus) of Euplotes focardii, a strictly coldadapted, Antarctic ciliate: one of 1,800 bp for α-tubulin and three of 2,150, 1,900, and 1,600 bp, respectively, for β-tubulin. Preliminarily analysed for restriction fragment length polymorphisms, these genes showed remarkable differences in organisation from tubulin genes of other ciliates which live in temperate areas and were analysed in parallel with E. focardii. The complete coding sequence of the 1,600 bp β-tubulin gene was then determined and shown to contain unique structural features of potential importance for E. focardii microtubule organization and activity. Of eight unique substitutions detected, seven were concentrated in the large amino terminal domain of the molecule that directly interacts with the carboxy terminal region of α-tubulin for heterodimer formation. Sequence analysis of the cloned gene revealed, in addition, a potential new exception in the use of the genetic code by ciliates. A TAG codon was aligned in correspondence with Trp-21 which is strictly conserved in every tubulin sequence so far determined.

Notes: . The mitochondrion appears to be essential for the growth of asexual, intraerythrocytic stages of Plasmodium falciparum and may thus be a suitable chemotherapeutic target. The in vitro activity of almitrine, a mitochondrial ATP synthetase inhibitor used for the treatment of hypoxemia, was compared with other mitochondrial inhibitors against chloroquine-susceptible and chloroquine-resistant P. falciparum using an isotopic semimicro drug susceptibility assay. The 50% inhibitory concentration (IC50) values of almitrine (range: 2.6–19.8 μM) were within similar range of values of other mitochondrial ATP synthetase inhibitors and doxycycline, a mitochondrial protein synthesis inhibitor. Almitrine was equally active against chloroquine-susceptible and chloroquine-resistant parasites. Drug combination studies showed no interaction between chloroquine and almitrine. Our results suggest that almitrine, a clinically safe drug, may represent a lead compound with a specific target against the mitochondrial ATP synthetase which may be useful for antimalarial chemotherapy.

Notes: . Twenty-five in vitro cultures of Giardia duodenalis derived from a Brisbane patient were established to assess the genetic heterogeneity of a population. Each of the established lines carried a predominance of one of two distinct varieties of Giardia. The two varieties were heterogeneous by four unambiguous criteria that were representative of the whole genome. These included restriction enzyme polymorphisms, hybridization with the cloned rDNA repeat and with a gene encoding a cysteine-rich surface protein, electrophoretic karyotyping and DNA fingerprinting. Differences between parasites derived from this patient were greater than have been seen between all other established G. duodenalis in vitro cultures from both human and animals. The culture were heavily selected such that a single Giardia line carried a predominance of one genotype and was not representative of the entire original population.

Notes: . Cross-transmission experiments were performed in order to determine the host specificity in the intermediate and definitive hosts of the four described dihomoxenous Sarcocystis species, S. gallotiae, S. stehlinii, S. simonyi, and S. dugesii from lacertid lizards of the genera Gallotia and Podarcis from the Macaronesian Islands. Sarcocysts of either species from experimentally infected lizards were fed to a variety of laboratory-bred lizard species of the genera Gallotia, Lacerta, and Podarcis. These sarcocysts proved to be infectious to all examined animals, showing no definitive host specificity in the tested genera. Lizards of the genera Chalcides and Tarentola, however, were not susceptible definitive hosts for S. gallotiae. The inoculation of experimentally obtained sporocysts of each of the four Sarcocystis species to various lacertid lizard species revealed varying degrees of intermediate host specificity, generally demonstrating each native host to be the most susceptible.

Notes: . Cells of the ciliate Tetrahymena thermophila failed to establish cultures in lipid-free standard synthetic nutrient medium if the initial population density was 250 cells per ml or less. These cells died within 10 h, but were saved and formed dense cultures if their medium was supplemented with 10 μg per ml of either certain phospholipids, 1,2-di-, 1-monoglycerides, fatty acids, long-chain alcohols, or sterols. Cell multiplication was followed in cultures in which the standard synthetic medium was supplemented with a selection of the compounds listed above. It was observed that the cells in the supplemented cultures in their exponential phases of growth had about the same average doubling times as control cells starting multiplication at 10-fold higher initial cell densities in lipid-free medium. These cells have been grown for decades in lipid-free synthetic nutrient media at short (ca. two-three h) doubling times. Therefore lipids have been considered nutritionally non-essential for growth and multiplication of these cells. We propose that those compounds that rescue the cells at low cell densities act as “proliferation signals,”sensu lato. This effect of lipids and long-chain alcohols has so far remained unnoticed.