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  • Cell & Developmental Biology
  • Elasticity
  • Inorganic Chemistry
  • American Association for the Advancement of Science (AAAS)  (3)
  • Technische Universität Clausthal-Zellerfeld, Math.-Naturwiss. Fakultät  (1)
  • 1990-1994  (4)
  • 1935-1939
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Keywords
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Years
Year
  • 1
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    American Association for the Advancement of Science (AAAS)
    Publication Date: 1994-11-11
    Description: Fluorescence-imaged micropipette aspiration was used to map redistribution of the proteins and lipids in highly extended human red blood cell membranes. Whereas the fluid bilayer distributed uniformly (+/- 10 percent), the underlying, solidlike cytoskeleton of spectrin, actin, and protein 4.1 exhibited a steep gradient in density along the aspirated projection, which was reversible on release from deformation. Quantitation of the cytoskeletal protein density gradients showed that skeletal elasticity is well represented by a grafted polymer network with a ratio of surface dilation modulus to shear modulus of approximately 2:1. Fractionally mobile integral proteins, such as band 3, and highly mobile receptors, such as CD59 as well as glycophorin C in protein 4.1-deficient cells, appeared to be squeezed out of areas dense in the underlying network and enriched in areas of network dilation. This complementary segregation demonstrates patterning of cell surface components by cytoskeletal dilation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Discher, D E -- Mohandas, N -- Evans, E A -- P01 DK32094-09/DK/NIDDK NIH HHS/ -- R01 DK26263-15/DK/NIDDK NIH HHS/ -- R01 HL31579-13/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1994 Nov 11;266(5187):1032-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Joint Graduate Group in Bioengineering, University of California, Berkeley, San Francisco 94720.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7973655" target="_blank"〉PubMed〈/a〉
    Keywords: Actins/blood ; Anion Exchange Protein 1, Erythrocyte/analysis ; Blood Proteins/*analysis ; *Cytoskeletal Proteins ; Cytoskeleton/chemistry/*physiology ; Elasticity ; *Erythrocyte Deformability ; Erythrocyte Membrane/*chemistry/physiology ; Erythrocytes/chemistry/cytology/*physiology ; Glycophorin/analysis ; Glycosylphosphatidylinositols/blood ; Humans ; Lipid Bilayers ; Membrane Lipids/*blood ; Membrane Proteins/analysis ; Microscopy, Fluorescence ; *Neuropeptides ; Spectrin/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 2
    Publication Date: 1992-11-13
    Description: Single DNA molecules were chemically attached by one end to a glass surface and by their other end to a magnetic bead. Equilibrium positions of the beads were observed in an optical microscope while the beads were acted on by known magnetic and hydrodynamic forces. Extension versus force curves were obtained for individual DNA molecules at three different salt concentrations with forces between 10(-14) and 10(-11) newtons. Deviations from the force curves predicted by the freely jointed chain model suggest that DNA has significant local curvature in solution. Ethidium bromide and 4',6-diamidino-2-phenylindole had little effect on the elastic response of the molecules, but their extent of intercalation was directly measured. Conversely, the effect of bend-inducing cis-diamminedichloroplatinum (II) was large and supports the hypothesis of natural curvature in DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Smith, S B -- Finzi, L -- Bustamante, C -- GM 32543/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1992 Nov 13;258(5085):1122-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Molecular Biology, University of Oregon, Eugene 97403.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1439819" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry, Physical ; Cisplatin/pharmacology ; DNA/*chemistry ; Elasticity ; Ethidium/pharmacology ; Glass ; Indoles/pharmacology ; Intercalating Agents/pharmacology ; *Magnetics ; Mathematics ; *Microspheres ; Physicochemical Phenomena
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 1992-09-25
    Description: Since its invention a few years ago, the atomic force microscope has become one of the most widely used near-field microscopes. Surfaces of hard sample are imaged routinely with atomic resolution. Soft samples, however, remain challenging. An overview is presented on the application of atomic force microscopy to organic samples ranging from thin ordered films at molecular resolution to living cells. Fundamental mechanisms of the image formation are discussed, and novel imaging modes are introduced that exploit different aspects of the tip-sample interaction for local measurements of the micromechanical properties of the sample. As examples, images of Langmuir-Blodgett films, which map the local viscoelasticity as well as the friction coefficient, are presented.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Radmacher, M -- Tillamnn, R W -- Fritz, M -- Gaub, H E -- New York, N.Y. -- Science. 1992 Sep 25;257(5078):1900-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Physikdepartment, Technische Universitat Munchen, 8046 Garching, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1411505" target="_blank"〉PubMed〈/a〉
    Keywords: Actin Cytoskeleton/ultrastructure ; Amino Acids ; Blood Platelets/cytology ; Crystallography ; Elasticity ; Humans ; Microscopy/*instrumentation ; Platelet Activation ; Proteins/ultrastructure ; Viscosity
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Technische Universität Clausthal-Zellerfeld, Math.-Naturwiss. Fakultät
    In:  Dissertation, Clausthal-Zellerfeld, Technische Universität Clausthal-Zellerfeld, Math.-Naturwiss. Fakultät, vol. 10, no. GL-TR-89-0259, pp. 277-280, (ISBN 0 08 042822 3)
    Publication Date: 1990
    Keywords: Rock mechanics ; Fracture ; Elasticity ; Finite Element Method ; Fluids ; salt ; diapir
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