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  • Articles  (7)
  • Lipids
  • 1990-1994  (5)
  • 1980-1984  (2)
  • Medicine  (7)
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  • Articles  (7)
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  • 1
    Electronic Resource
    Electronic Resource
    Lipid transfer in plants (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 579-585 
    Details
    ISSN: 1420-9071
    Keywords: Lipids ; phospholipids ; galactolipids ; fatty acids ; transfer ; binding ; membranes ; mitochondria ; chloroplasts ; biogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Plant cells contain cytosolic proteins, called lipid transfer proteins (LTP), which are able to facilitate in vitro intermembrane transfer of phospholipids. Proteins of this kind from three plants, purified to homogeneity, have several properties in common: molecular mass around 9 kDa, high isoelectric point, lack of specificity for phospholipids, and binding ability for fatty acids. The comparison of their amino acid sequences revealed striking homologies and conserved domains which are probably involved in their function as LTPs. These proteins could play a major role in membrane biogenesis by conveying phospholipids from their site of biosynthesis to membranes unable to form these lipids. Immunochemical methods were used to establish an in vivo correlation between membrane biogenesis and the level of LTP or the amount of LTP synthesized in vitro from mRNAs. The recent isolation of a full-length cDNA allows novel approaches to studying the participation of LTPs in the biogenesis of plant cell membranes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01939696
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of essential oil ofHyssopus officinalis on the lipid composition ofAspergillus fumigatus (1994)
    Springer
    Mycopathologia 126 (1994), S. 163-167 
    Details
    ISSN: 1573-0832
    Keywords: Aspergillus fumigatus ; Essential oil ; Growth ; Lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Addition of the essential oil ofHyssopus officinalis to the culture medium ofAspergillus fumigatus induced alterations in both growth and lipid composition of this mould. Total lipids and sterols were reduced, whereas total phospholipids were increased. There were alterations in the proportions of fatty acids, neutral lipid and phospholipid fractions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103770
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of amphotericin B on the lipids of five different strains ofCryptococcus neoformans (1994)
    Springer
    Mycopathologia 128 (1994), S. 85-89 
    Details
    ISSN: 1573-0832
    Keywords: Amphotericin B ; Cryptococcus neoformans ; Lipids ; Sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cells of five strains ofCryptococcus neoformans were obtained for partial analysis of lipid composition. Quantitative analysis of lipids and sterols were completed, as well as qualitative analysis of sterols by thin-layer chromatography and by the ultraviolet spectra. Such determinations were made on cells cultured in the absence and presence of amphotericin B at sub-MIC (minimal inhibitory concentration) levels. Marked alterations of the lipid and sterol contents were observed in the amphotericin B — treated cells. Moreover, ergosterol disappeared in these antibiotic-exposed cells. It is concluded that amphotericin B altered the lipid profiles, especially sterols ofC. neoformans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103014
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  • 4
    Electronic Resource
    Electronic Resource
    Lipid transport pathways in mammalian cells (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 569-579 
    Details
    ISSN: 1420-9071
    Keywords: Lipids ; membranes ; transport ; organelles ; vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A major deficit in our understanding of membrane biogenesis in eukaryotes is the definition of mechanisms by which the lipid constituents of cell membranes are transported from their sites of intracellular synthesis to the multiplicity of membranes that constitute a typical cell. A variety of approaches have been used to examine the transport of lipids to different organelles. In many cases the development of new methods has been necessary to study the problem. These methods include cytological examination of cells labeled with fluorescent lipid analogs, improved methods of subcellular fractionation, in situ enzymology that demonstrates lipid translocation by changes in lipid structure, and cell-free reconstitution with isolated organelles. Several general patterns of lipid transport have emerged but there does not appear to be a unifying mechanism by which lipids move among different organelles. Significant evidence now exists for vesicular and metabolic energy-dependent mechanisms as well as mechanisms that are clearly independent of cellular ATP content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01939695
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  • 5
    Electronic Resource
    Electronic Resource
    Lipid composition of two types of chondrocytes in primary culture (1981)
    Springer
    Calcified tissue international 33 (1981), S. 403-407 
    Details
    ISSN: 1432-0827
    Keywords: Chondrocytes ; Articular cartilage ; Epiphyseal cartilage ; Primary culture ; Lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Chondrocytes from articular and growth plate cartilage were grown in primary culture. The lipid content, distribution into different types, as well as the fatty acid patterns of these lipids were compared when the cells had reached stationary phase and were synthesizing maximal amounts of proteoglycans. Numerous significant differences were observed, depending on the origin of the chondrocytes. In particular, growth plate chondrocytes showed increased dry weight, increased lipid content (phosphatides and triglycerides), and decreased cholesterol to phosphatide ratio when compared to articular chondrocytes; they also incorporated more of C18:1 and less C16:0 into their major lipid types. Whether these differences arise from specific metabolic regulation or are a consequence of chondrocyte organization in primary culture remains unclear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409463
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  • 6
    Electronic Resource
    Electronic Resource
    Lipid and apolipoprotein levels during therapy with pinacidil combined with hydrochlorothiazide (1992)
    Springer
    European journal of clinical pharmacology 42 (1992), S. 65-70 
    Details
    ISSN: 1432-1041
    Keywords: Lipids ; Pinacidil ; Hydrochlorothiazide ; hypertension ; triglyceride ; cholesterol ; apolipoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary This study determined the effect of pinacidil on the concentration of plasma lipids and apolipoproteins in male patients previously equilibrated with 25 mg hydrochlorothiazide twice daily. Pinacidil therapy given to 52 hypertensives at 25 to 100 mg daily for 8 weeks resulted in a reduction of systolic and diastolic blood pressure concurrently to reductions in plasma cholesterol and triglycerides with no change in low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C). There was an associated decrease in apolipoproteins (Apo)B, C-III and E and elevation in ApoA-I. A parallel placebo group of 44 patients experienced reduction in diastolic blood pressure and an elevation in ApoA-I. These changes indicate that pinacidil will be a useful antihypertensive agent having properties on lipoprotein metabolism which would favor decreased risks of atherosclerosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314922
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  • 7
    Electronic Resource
    Electronic Resource
    Lipid storage in cultured articular chondrocytes due to prostanoid precursors and a prostanoid synthesis inhibitor (1982)
    Springer
    Cell & tissue research 224 (1982), S. 441-448 
    Details
    ISSN: 1432-0878
    Keywords: Chondrocyte culture ; Prostanoid precursors ; Indomethacin ; Lipids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lapine articular chondrocytes were subcultured in the presence or absence of the prostanoid precursors, arachidonic acid or dihomo-gammalinolenic acid, and the cyclooxygenase inhibitor indomethacin. Lipid storage was studied microscopically using the Sudan black staining method. Control chondrocyte cultures showed a weakly positive staining reaction until confluence was reached, at which point the intra-cytoplasmic lipid content decreased. Both arachidonic acid and dihomo-gamma-linolenic acid at 100 μmol/l caused a marked increase in lipid storage which continued even after confluence was achieved. 1 μmol/l concentrations were indistinguishable from controls, whereas 10 μmol/l concentrations elicited a slight increase in lipid storage compared with controls. The prostaglandin cyclooxygenase inhibitor indomethacin did not affect chondrocyte lipid storage. However, administration of a prostanoid precursor in the presence of indomethacin caused a massive increase in intra-cytoplasmic storage of lipid, eventually leading to cell death. A possible explanation is that indomethacin may alter chondrocyte lipid metabolism in the presence of substrate molecules by rechanneling lipid synthesis away from the prostaglandin pathway to other lipid synthetic pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216885
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