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  • RFLP  (260)
  • Springer  (260)
  • Cambridge University Press
  • 1995-1999  (260)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Molecular identification of co-occurring Cortinarius and Dermocybe species from southeastern Australian sclerophyll forests (1999)
    Springer
    Mycorrhiza 9 (1999), S. 85-90 
    Details
    ISSN: 1432-1890
    Keywords: Key words PCR ; RFLP ; ITS sequence analysis ; Ectomycorrhizal fungi ; Cortinarius taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The ability of restriction fragment length polymorphism (RFLP) analysis of the rDNA internal transcribed spacer (ITS) region to discriminate 10 co-occurring Cortinarius and Dermocybe species at a southeastern Australian sclerophyll forest site was assessed. Using the basidiomycete-specific primers ITS1F and ITS4B, some taxa were separated on the basis of individual RFLP patterns derived using the restriction endonucleases Hae III or Hinf I. Combined data from both endonucleases were, however, required to separate all taxa [Dermocybe austro-veneta Clel. (Moser & Horak), C. rotundisporus Clel. & Cheel, C. archeri Berk., C. sinapicolor Clel., C. violaceus (L.: Fr.) S.F.Gray, C. radicatus Clel. and four morphologically-distinct, but unidentified Cortinarius spp.]. ITS sequence comparisons confirmed that D. austro-veneta belongs in Dermocybe, that C. rotundisporus is correctly placed in subgenus Phlegmacium, and suggest that Australian C. violaceus collections are not conspecific with northern hemisphere C. violaceus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s005720050004
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  • 2
    Electronic Resource
    Electronic Resource
    Sex in Escherichia coli does not disrupt the clonal structure of the population: evidence from random amplified polymorphic DNA and restriction-fragment-length polymorphism (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 440-448 
    Details
    ISSN: 1432-1432
    Keywords: Escherichia coli ; RAPD ; RFLP ; Clonal theory ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of the Escherichia coli population by multilocus enzyme electrophoresis (MLEE) has established its clonal organization, but there is increasing evidence that horizontal DNA transfer occurs in E. coli. We have assessed the genetic structure of the species E. coli and determined the extent to which recombination can affect the clonal structure of bacteria. A panel of 72 E. coli strains from the ECOR collection was characterized by random amplified polymorphic DNA (RAPD) and restriction-fragment-length polymorphism (RFLP) of the ribosomal RNA gene (rrn) regions. These strains have been characterized by MLEE and are assumed to reflect the range of genotypic variation in the species as a whole. Statistical analysis, including factorial analysis of correspondence (FAC) and hierarchical classifications, established that the data obtained with the three genetic markers are mutually corroborative, thus providing compelling evidence that horizontal transfer does not disrupt the clonal organization of the population. However, there is a gradient of correlation between the different classifications which ranges from the highly clonal structure of 132 group strains causing extraintestinal infections in humans to the less-stringent structure of B1 group strains that came mainly from nonprimate mammals. This group (B1) appears to be the framework from which the remaining non-A group strains have emerged. These results indicate that RAPD analysis is well suited to intraspecies characterization of E. coli. Lastly, treating the RAPD data by FAC allowed description of subgroup-specific DNA fragments which can be used, in a strategy comparable to positional cloning, to isolate virulence genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160315
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  • 3
    Electronic Resource
    Electronic Resource
    Comparative Analysis of Different DNA Extraction Protocols: A Fast, Universal Maxi-Preparation of High Quality Plant DNA for Genetic Evaluation and Phylogenetic Studies (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 69-86 
    Details
    ISSN: 1572-9818
    Keywords: cpDNA ; DNA extraction ; fingerprinting ; forest trees ; M13 fingerprinting ; method ; PCR ; rDNA ; RFLP ; rhododendron ; plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four DNA extraction protocols were compared for ability to produce DNA from the leaves or needles of several species: oak, elm, pine, fir, poplar and maize (fresh materials) and rhododendron (silica dried or frozen material). With the exception of maize and poplar, the species are known to be difficult for DNA extraction. Two protocols represented classical procedures for lysis and purification, and the other two were a combination of classical lysis followed by anion exchange chromatography. The DNA obtained from all procedures was quantified and tested by PCR and Southern hybridisation.Test results indicated superiority of one of the four protocols; a combination of CTAB lysis followed by anion exchange chromatography which enabled DNA extraction from all seven species. A second protocol also produced DNA from leaves or needles of all species investigated and was well suited for PCR applications but not Southern hybridisations. The remaining protocols produced DNA from some but not all species tested. Abbreviations: CTAB, hexadecyltrimethylammonium bromide; EtOH, Ethanol; TBE, tris-borate-EDTA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007428009556
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  • 4
    Electronic Resource
    Electronic Resource
    A Rapid DNA Extraction Method for RFLP and PCR Analysis from a Single Dry Seed (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 90-90 
    Details
    ISSN: 1572-9818
    Keywords: DNA extration ; DNA fingerprint ; half seed ; PCR ; RFLP ; target gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A single-seed DNA extraction method was developed for rapid identification of plant genotype. The method was applied to 12 plant species, including the oil seeds sesame and soybean. The results were comparable to those obtained for oil-less seeds such as rice. This method will be useful for genotypic selection which requires rapid screening of large populations. It can also be used to identify varietal purity of seed stocks by PCR and RFLP analysis. The method includes two major steps, (i) treatment by proteinase K in an SDS extraction buffer, and (ii) grinding of a single half seed in the buffer after incubation. About 1.5–2 µg of DNA per half seed (the endosperm part) of rice was obtained and more than 200 half seed samples could be handled by one person in a day. The DNA could be used for fingerprinting and detection of target genes in a transgenic plant by PCR. The amplified PCR products from the half seed DNA exhibited the same banding patterns as those from leaf DNA. Yield and quality of DNA extracted from half seeds of rice was also sufficient for RFLP analysis. The remnant half seeds containing the embryo can be maintained for later germination of selected genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007418606098
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  • 5
    Electronic Resource
    Electronic Resource
    A new polymorphism in the 5' flanking region of the human interleukin (IL)-4 gene (1999)
    Springer
    Immunogenetics 49 (1999), S. 738-739 
    Details
    ISSN: 1432-1211
    Keywords: Key words Interleukin (IL)-4 ; Promoter ; Polymorphism ; RFLP ; Linkage disequilibrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050677
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  • 6
    Electronic Resource
    Electronic Resource
    A new polymorphism in the promoter of the Interleukin 5 receptor alpha subunit (IL-5RA) gene (1998)
    Springer
    Immunogenetics 48 (1998), S. 65-66 
    Details
    ISSN: 1432-1211
    Keywords: Key words IL-5Rα ; Promoter ; Polymorphism ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002510050402
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural and molecular study of plastid inheritance in Abies alba and some Abies hybrids (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 284-291 
    Details
    ISSN: 1432-2145
    Keywords: Key words Abies ; Egg cell ; Plastid inheritance ; RFLP ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The ultrastructure of egg cells in Abies alba was examined to elucidate the lack of maternal inheritance of plastids. Before fertilization, maternal plastids are absent in the perinuclar zone containing mainly mitochondria and smooth endoplasmic reticulum. During egg cell development the maternal plastids are transformed into large inclusions which are situated mostly towards the periphery of the egg cell, and finally disintegrate. As a consequence, they do not participate in zygote formation. RFLP analysis of cpDNA of parental trees and their F1 interspecific hybrids (A. alba×A. numidica, A. alba×A. nordmanniana, A. nordmanniana×A. Alba) using HindIII and BamHI showed a paternal mode of cpDNA inheritance. Paternal inheritance has also been found with PCR/RFLP analysis of cpDNA from parental trees and their hybrids (A. alba×A. pinsapo, A. pinsapo×A. alba, A. pinsapo×A. numidica) using ApaI and HaeIII digests, as well as in the crosses of A. cephalonica×A. nordmanniana, A. nordmanniana×A. cephalonica, A. cephalonica×A. numidica using TagI digests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050155
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  • 8
    Electronic Resource
    Electronic Resource
    Non-Mendelian and skewed segregation of DNA markers in wide crosses of the bean rust fungus,Uromyces appendiculatus (1996)
    Springer
    Current genetics 29 (1996), S. 159-167 
    Details
    ISSN: 1432-0983
    Keywords: Genetic linkage mapping ; Segregation distortion ; RAPD ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The inheritance of DNA markers was investigated in 27 F2 progeny from a single F1 hybrid derived from a wide cross inUromyces appendiculatus. This cross was unusual because asexual spores were used to fertilize sexual fruiting structures. Sixty percent of the DNA markers failed to segregate according to simple Mendelian ratios. Segregation bias was evident, in that F2 progeny inherited on average 91 % of maternal bands and 52% of paternal bands, which deviates significantly from the expected value for each of 75% for dominant markers. Because of these distortions, linkage mapping was not possible with this population. Evaluation of two F1s from a second wide cross, reciprocals obtained by normal fertilization, also showed non-Mendelian inheritance of one of three co-dominant RFLPs and five of six isozyme markers, indicating that the method of crossing was probably not responsible for the abnormal segregation patterns in the first cross. Either genetic incompatibility, similar to that of an interspecific cross, or selection of particular genotypes could explain the genetic anomalies reported here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02221580
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  • 9
    Electronic Resource
    Electronic Resource
    Heterogeneity in intergenic regions of the ribosomal repeat of the pine-blister rustsCronartium flaccidum andPeridermium pini (1996)
    Springer
    Current genetics 29 (1996), S. 388-394 
    Details
    ISSN: 1432-0983
    Keywords: Cronartium flaccidum ; Pine blister rusts ; Ribosomal intergenic region polymorphism ; RFLP ; HPA analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mixed aeciospore isolates ofCronartium flaccidum andPeridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS 1 and ITS2 were found to be highly similar betweenC. flaccidum andP. pini. Within samples heterogeneity was detected at three nucleotide loci in the ITS1 and at four loci in the ITS2 suggesting that several fungal genotypes may occur at a single infection court. The heterogeneity was confirmed by heteroduplex polymorphism analysis of mixed aeciospore products. RFLP of the ribosomal intergenic spacer region 1 (IGSI) amplified from the same templates indicated limited sequence polymorphism in some copies of this repeated locus. Both the sexual and asexual forms ofC. flaccidum show evidence of sequence polymorphism in two independent, non-coding regions of the ribosomal gene array. Variation appears to be greater in the sexual formC. flaccidum, than in the monoaecious formP. pini.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208620
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  • 10
    Electronic Resource
    Electronic Resource
    Heterogeneity in intergenic regions of the ribosomal repeat of the pine-blister rusts Cronartium flaccidum and Peridermium pini (1996)
    Springer
    Current genetics 29 (1996), S. 388-394 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Cronartium flaccidum ; Pine blister rusts ; Ribosomal intergenic region polymorphism ; RFLP ; HPA analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples heterogeneity was detected at three nucleotide loci in the ITS1 and at four loci in the ITS2 suggesting that several fungal genotypes may occur at a single infection court. The heterogeneity was confirmed by heteroduplex polymorphism analysis of mixed aeciospore products. RFLP of the ribosomal intergenic spacer region 1 (IGS1) amplified from the same templates indicated limited sequence polymorphism in some copies of this repeated locus. Both the sexual and asexual forms of C. flaccidum show evidence of sequence polymorphism in two independent, non-coding regions of the ribosomal gene array. Variation appears to be greater in the sexual form C. flaccidum, than in the monoaecious form P. pini.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050060
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