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  • Articles  (9,681)
  • Springer  (8,723)
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  • Emerald  (301)
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  • American Association for the Advancement of Science (AAAS)
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  • Oxford University Press
  • 1995-1999  (9,681)
  • Process Engineering, Biotechnology, Nutrition Technology  (9,681)
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  • Articles  (9,681)
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  • 1
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 24-28 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A three-year consumer research programme, using both qualitative and quantitative techniques, was undertaken to assess whether additional voluntary nutrition information for calories and fat would aid consumers' ability to use the nutrition information that is given on food packs. A variety of label formats was investigated. The research established that providing information for calories and fat per serving, either separately from the nutrition panel or highlighted within the nutrition panel, and providing Guideline Daily Amounts (GDAs) for calories and fat helped to make the nutrition information more accessible to consumers. GDAs were felt by consumers to be new and useful information. The research findings were developed into voluntary industry guidelines by an IGD working group consisting of representatives of manufacturers, retailers, consumer organizations, nutrition scientists and government. The guidelines represent best practice for industry.
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  • 2
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The purpose of this study was to determine body composition, energy balance and tendencies towards eating disorders of female competitive athletes in two countries, USA and Greece. Data were collected using similar methodology in both countries during the non-competitive (training) and the competitive seasons. Forty-two athletes and 11 controls in the USA and 35 athletes and ten controls in Greece participated in the study. Comparison of the results between the countries showed that US athletes weighed more than Greek athletes but there was no significant difference in the percentage of body fat between the two groups. No significant differences were found in the energy intakes between athletes in either country. Athletes appeared to be in negative energy balance (-380 to -580 kcals daily) in both seasons, in both countries.
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  • 3
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    Electronic Resource
    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 12-18 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A survey of 12 residential homes assessed the nutritional balance of the meals selected and consumed by the elderly residents. With the increasing age of the population and the demand for professional care there is an increasing pressure on the professional caterer and carer to meet the nutritional needs of those in their care. However the ongoing concern over the safety of various foods and the requirement for residential homes to remain within budget can have an impact on the food offered to and consumed by the elderly person. The results show a general satisfaction by the consumer with the food supplied but the research indicates that the meals are not generally nutritionally balanced. The overall concern of the providers is to remain within budget and any nutritional balance of meals is obtained more by accident than as a planned policy. If this is an indication of the situation in Northern Ireland there is the possibility that throughout the United Kingdom there may be similar widespread imbalance in the nutritional provision to the older members of the population.
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  • 4
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 37-41 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A good relationship between the hospitality industry and environmental health officers is seen as essential if food poisoning statistics are to be improved in the future. A questionnaire issued to both parties indicates that there are still areas of disagreement which need to be bridged if progress in food safety is to be made.
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  • 5
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 78-80 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With changes in the curriculum, science breakthroughs, consumer demands and the Government's recent Green Paper on Our Healthier Nation, the British Nutrition Foundation's National Nutrition Education Conference entitled "Emerging issues for the next millennium" informed delegates about emerging issues in nutrition and education.
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  • 6
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 144-148 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The role of diet in contributing to the incidence of coronary heart disease, cancer and other common Western diseases is attracting increasing attention. Foods which provide different biologically active compounds, such as phyto-oestrogens, have been extensively studied and epidemiological evidence suggests that populations consuming a diet high in phyto-oestrogens have a lower risk of these diseases. This paper reviews some of the key studies on soy isoflavones which suggest that the incorporation of soy protein into the British diet may offer important health benefits. Non-steroidal dietary oestrogens are found in high concentrations in the traditional diets of the Far East, and by vegans who consume large amounts of soy protein. Consumption of isoflavones across Asia varies considerably and it is estimated that between 25 to 45mg of isoflavones is consumed daily, compared to as little as 5mg daily in Western countries. It is well established that modification of dietary fat, especially saturated fat, soluble fibre and total calories have a role in coronary heart disease risk. In a meta-analysis of 38 controlled trials on soy proteins, it was concluded that significant reductions in serum and total LDL cholesterol could be achieved. A wide range of epidemiological data shows strong correlations between a diet high in soy protein and vegetables and a reduced risk of breast cancer. The incidence of osteoporosis in Japanese women is significantly lower than in Western women and dietary factors such as soy protein intake may be important in sustaining this protection.
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  • 7
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 187-193 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The links between diet, bacteria and colonic cancer are examined in this article. Studies suggest that high intakes of fat and protein are associated with elevated risk of colonic cancer whereas cereals, fruits and vegetables seem to be protective. A further aspect considered in relation to this type of cancer is metabolism by gut bacteria. Probiotics, prebiotics and synbiotics are presented as ways of stimulating the activities, certain gut flora and the use of "functional foods" is also discussed.
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  • 8
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 219-224 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new National Diet and Nutrition Survey was published in October 1998, by the Ministry of Agriculture, Fisheries and Food and the Department of Health, which reported on the dietary intake and nutritional status of people over 65 years of age in the UK. This survey provided data on 1,275 free-living people and 412 people in residential care. Along with the information on diet and nutritional status are data on anthropometric and biochemical parameters. A second related survey looked at dental health in this age group. The key findings of these surveys and the public health implications for older people are discussed.
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  • 9
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 178-184 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Antibiotics are used in animals to cure disease and prevent suffering. Bacterial populations in animals which are exposed to these antibiotics may be selected for resistance. There is a concern that spread of these bacteria in the food chain to humans may result in the establishment of antibiotic resistant bacteria in people which will prove difficult or impossible to treat with conventional drugs. It is clear that antibiotics must be used carefully to reduce the development of resistance and that steps should be taken to reduce the transfer of bacteria from animals to man.
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  • 10
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    Nutrition & food science 99 (1999), S. 192-199 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article focuses on the dietary requirements of people with Diabetes Mellitus, both insulin dependent and non-insulin dependent. It is one disorder where good dietary management can benefit control, well being and avoid complications. The guidelines for dietary intake for those with Diabetes Mellitus both past and present are given. The recommended intake of carbohydrate, fruit and vegetables, sugar, protein, fat, salt and alcohol are also discussed. The study itself focuses on the organisations involved in producing diet sheets for people with Diabetes Mellitus. The article also gives information on how to obtain such publications.
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  • 11
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 229-238 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Few studies have concentrated on the food choices of young people and the potential influences, yet the food choices established in these early years can determine the diet and quality of health in later life. This study investigates the diet and food choices of 9-17-year olds in Northern Ireland and considers the potential effects of age, gender and socio-economic grouping. A range of research methods was implemented including observations, questionnaires and diet based case studies. The observations, in five schools, indicated the types of foods being chosen whilst the questionnaires further investigated this initial information along with other factors, to obtain more detailed data. A total of 764 questionnaires were administered to young people and 516 to parents to facilitate comparison. In-depth diet based case studies also took place evaluating the diets of 14 young people. The research indicated that this group have a diet which continues to cause concern.
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  • 12
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 270-277 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Outlines the numerous messages sent to consumers regarding healthy eating practices and indicates the problems involved in determining whether or not the British consumer has adopted a nutritious and healthy diet. Research was undertaken which measured individuals' involvement in healthy eating issues and related this to several demographic characteristics. The research indicates some variation in involvement in healthy eating according to demographic variables but the results were not statistically significant. Group discussions were also conducted which confirmed that the healthy eating messages were widely received and understood; however, there was some confusion over their content, scepticism regarding the veracity and motivation of some sources and a growing resentment of boring and puritanical themes. In the future care must be taken to produce clear, simple and positive healthy eating messages to the public if trends towards a more nutritious diet are to continue.
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  • 13
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 281-284 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Much of the debate surrounding genetic modification has centred on food product ingredients, in particular soya derivatives, and the supposed inability to segregate GM from non-GM soybeans. Protein Technologies International has, however, implemented a system, Identity Preservation, which ensures the delivery of non-GM soy protein to its customers. The system covers seeds, on-farm storage, planting, growing and harvesting, transportation, processing and distribution, with independent third-party verification. It is, believes the company, a way of ensuring that consumers can obtain the health benefits of soy protein consumption even if they are actively avoiding GM ingredients.
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  • 14
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 140-143 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: foodlink's National Food Safety Week provides a focus for communicating messages designed to help people understand and initiate the basic precautions which can be taken to reduce the risk of food poisoning and in the process provide fun and entertainment for thousands throughout the UK. There were over 100,000 reported cases of food poisoning last year and there has been much speculation recently that many more cases go unreported. National Food Safety Week provides the tools both to raise awareness of the problem and to provide the answers in a fun, inclusive and effective way. Indeed, keeping food safe is one of the most basic applications of technology in the home.
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  • 15
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 173-179 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article outlines the importance of increasing fruit and vegetable intake in the battle against diet-related disease and health inequalities. It identifies some of the barriers to increasing fruit and vegetable consumption and proposes some potential solutions.
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  • 16
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 181-186 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Packed lunches are a popular form of midday meal for children at school. This study of 11-12-year-old children in Northern Ireland found sandwiches, crisps, chocolate and carbonated drinks to be popular items included in a packed lunch. Dairy produce and fruit were poorly represented. The nutritional quality varied with the type of lunch. The contribution of fat and saturated fatty acids to total energy intake were in all cases far in excess of recommended dietary guidelines. Such high intakes of fat militate against the reduction in the incidence of coronary heart disease in Northern Ireland. The intake of dietary fibre and several micronutrients were inadequate.Knowledge of healthier foods was not translated into food consumption. If major diseases in the longer term are to be minimised a challenge exists for those responsible for food preparation ± parents, food processors and suppliers in the hospitality industry to produce foods which are healthier while not adversely affecting their acceptability to children.
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  • 17
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 224-230 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Discusses the role appearance plays in influencing consumer perception and subsequent acceptance of a food product. Texture and flavour have long since been known to exert an effect on consumer perception. However, the "first taste is almost always with the eye". This is especially the case where a food product is sold through its appearance, rather than through its packaging. In addition, appearance can have a halo effect which modifies subsequent flavour perception and food acceptability. The article also briefly discusses the many factors that affect the perception process and the significance of food product development in the food industry.
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  • 18
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 243-248 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper highlights the effectiveness of TCS (the Teaching Company Scheme) in improving the performance and competitiveness of companies in the food-processing industry. The industry is swinging towards the more efficient production of safe, high-quality foods, a change propelled by UK and EC legislation and by market forces. Developing and implementing new process procedures and systems, exploring and developing novel ingredients, new products and market sectors are projects that require heavy investment of time, resources and personnel. However, many small and medium-sized food-processing companies lack the resources necessary to carry out such strategic projects. TCS is a highly successful government scheme that stimulates innovation and facilitates technology and knowledge transfer in most business and industry sectors, including the food industry, by supporting partnerships between companies and the UK's knowledge base (universities and research organisations and institutions). It also enables companies to recruit and train high-calibre graduates seeking careers in industry.
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  • 19
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    Nutrition & food science 99 (1999), S. 131-135 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article highlights current research at the Leatherhead Food Research Association which is relevant to shelf-life assessment of food products. One area is to improve the quality and safety of fish - both fresh and cold-smoked. Food MicroModel, a computer software package which aids in the prediction of the microbiological safety and spoilage potential of food products, is described. New methods capable of predicting the stability of oils and fats to oxidation, which can be a significant cause of reduced shelf-life in fat-containing foods, are described. Novel analytical instruments, known as electronic noses, have been shown to have potential, as has a total antioxidant activity test. Temperature control in factories is often a compromise between product safety and the need to maintain reasonable employee working conditions. This is no longer a problem with the development of glove box technology, which separates the two environments. The final study discussed in this article is the development of accelerated shelf-life tests for chocolate products.
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  • 20
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    Nutrition & food science 99 (1999), S. 170-174 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A quantitative method involving colony counting was undertaken to assess the antibiotic effects of garlic Allium sativum on possible food-borne bacteria. The investigation using garlic was targeted at higher education students planning an Independent Study or Dissertation into the antibacterial properties of food plants. The effect of concentrations of garlic varying from 0 per cent to 20 per cent in chicken stock, which had been inoculated with either E.coli B and S. albus, was investigated over three days. Bacterial numbers were reduced with increased garlic concentration and with the duration of incubation. Heating the garlic for 30 min at 100 C destroyed its anti-bacterial activity.
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  • 21
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    Bingley : Emerald
    Nutrition & food science 99 (1999), S. 237-243 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The recent arrival of the UK multiples has had a significant effect on the Northern Ireland market, particularly in terms of product range and choice. Ethnic foods offer a potentially lucrative sector, as evidenced in Great Britain. However, retailers in Northern Ireland are only beginning to develop range and ethnicity and are familiarising themselves with a market which has been characterised more often by traditional eating habits. The preliminary study considered consumer perceptions of ethnic food products in Northern Ireland. It would appear that product awareness needs to be developed carefully, and the onus is on the retailer (and indeed, the hospitality establishments) to inform consumers and respond to their needs and wants, particularly with the more recent trend towards regional cuisine and greater authenticity. Only then can a larger range of truly ethnic food products penetrate the Northern Ireland market successfully.
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  • 22
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    Nutrition & food science 99 (1999), S. 278-280 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: FOOD MATTERS is an award scheme that aims to encourage healthy eating amongst pupils in Hertfordshire schools. The FOOD MATTERS Award seeks to encourage a whole school approach to healthy eating. To achieve the Award, schools must carry out the following: set up a FOOD MATTERS co-ordination group; gain the FOOD MATTERS catering certificate by meeting set catering criteria; and identify and achieve targets regarding the promotion of healthy eating in school. Schools participating in FOOD MATTERS have carried out many different projects. These include a pupil-run healthier tuck shop; a "try it and you might like it" day (to encourage primary school pupils to eat more fruit and vegetables); the introduction of "fruit smoothies", (to increase pupils' intake of fruit and high-calcium foods); a fruit-sharing scheme; and the installation of new drinking fountains.
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  • 23
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    Nutrition & food science 99 (1999), S. 288-294 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article focuses on the consumption of fish in Qatar and its health benefits for those living in the region. The nutritional value of fish from traditional fishing areas such as the UK has been much discussed but this article addresses the value of fish from warmer regions. Samples of popular fish were purchased from the local market in Qatar and were then analysed for levels of major nutrients and fatty acids. The likely nutritional impact of fish on the local diet was then evaluated. Problems of preservation of fresh fish, hygiene and mercury levels are also pointed out. Vigilance on behalf of the Public Health Authorities is urged to ensure the good reputation of fish in this region remains.
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  • 24
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    Nutrition & food science 99 (1999), S. 295-302 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The objectives of the study are the standardization of ten Nigerian dishes and the determination of their proximate composition. Recipes for the ten dishes were obtained through the use of questionnaires. There were 200 respondents to the questionnaires consisting of housewives living in the major towns of south-western Nigeria. One recipe for each meal was extracted from a known cookery book. The means for each ingredient of all the dishes were calculated from ten randomly selected recipes and then used in the preparation of the standardized dishes. The standardized dishes are: Burabisko, Jollof rice, Agbono soup, Stewed beans and fried plantain, Bean pudding, Melon seed and vegetable soup, Ikokore, Eba imoyo, Yam and eggs and Yam pottage. Consumer acceptability tests were conducted as well as proximate analyses according to AOAC. All dishes were considered acceptable since they scored more than 3, the benchmark for acceptability. Energy content of the dishes ranged from 281Kcal/100g (Jollof rice) to 510Kcal/100g (Agbono soup). The crude protein content ranged from 7.5g/100g for Burabisko to 27.4g/100g for Jollof rice. The results suggest that these dishes are good sources of energy and protein.
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  • 25
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    Nutrition & food science 99 (1999), S. 5-11 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article briefly outlines the main objectives of the Ministry of Agriculture, Fisheries and Food (MAFF) programme of research in support of dietary surveys. It summarises the current portfolio of projects, then discusses in more depth the six projects being undertaken on further analysis of data from government dietary surveys, drawing on papers presented by the researchers at the annual meeting of this programme in London on 2 June 1998.
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  • 26
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    Nutrition & food science 99 (1999), S. 19-23 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Investigates the effects of ingredients and processing procedure on colour and appearance properties of chilled dairy dessert products, namely mousse. Chilled mousse products were formulated via a factorial design involving several ingredients and processing factors. Sixteen formulated dairy dessert mousses were presented to a trained sensory panel. A screening experiment was carried via a fractional factorial design involving eight factors at two levels. The effects were examined by means of graphical half normal plots using the software Design Ease (Stat-Ease Inc., USA). Five factors were identified as being the more significant factors which were cream level (CRE), mix time (MIX), blue (BLU), yellow (YEL) and red (RED) colouring agent levels. A further full factorial formulation design was carried out involving four factors: CRE, MIX, BLU, RYR (ratio of red to yellow additive) in a series of sensory perception experiments. Results verified by multivariate analysis of variance (MANOVA) indicated that it was the level of cream and colouring agents that were the most significant factors (p〈0.001) affecting colour and appearance aspects of chilled mousse.
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  • 27
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    Nutrition & food science 99 (1999), S. 29-36 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Manufacturers are producing an extensive range of added value products which are formulated using meat replacers but which are designed to appeal to a wide range of consumers, above and beyond the "traditional vegetarian" market. This study considered the relatively recent impact of such products on the Northern Ireland market, with a particular emphasis on the quality and acceptability of Quorn based products. A small- scale questionnaire (n = 100) considered customer perceptions of meat replacers, whilst the acceptance of selected tofu, textured vegetable protein (TVP) and Quorn products was measured using selected sensory evaluation techniques. The study concluded that Quorn can offer similar texture and flavour attributes to those consumers who wish to avoid meat products for health and/or safety reasons. It is this customer base which needs to be targeted, but it must be noted that negative perceptions of meat replacers still exist. Therefore, further developments need to be supported by appropriate marketing strategies which will both attract and educate consumers and help to achieve a sustained level of purchasing.
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  • 28
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    Nutrition & food science 99 (1999), S. 72-77 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The food and nutrient intake of 250 (135 male, 115 female) adolescent Nigerian high school students have been surveyed to determine the contributions of different food groups to their intakes of protein, calcium and iron. Twenty-four-hour dietary recall technique was used to obtain details of food intake and questionnaires administered to obtain social and economic circumstances of subjects' family. Male subjects had more energy, protein, calcium and iron intake but no significant (p 〈 0.05) differences when compared with females. Cereal based foods were the most important sources of dietary protein, supplying between 40-52 per cent for all subjects and 60 per cent or more for 29 per cent of the subjects. Proportions of dietary protein from legumes, meats, vegetables and roots ranged from10-21 per cent, 3-13 per cent, 7-17 per cent, 6.5-12.9 per cent respectively. Cereals were also the most important source of dietary calcium (56.8 per cent) and iron (33.16 per cent) for most subjects. The contribution of meats and legumes however, approximate that of cereals for 36 per cent of subjects. Apart from gender, residence at home or in boarding house and the socio-economic status of the subject's family significantly affect the sources of dietary protein, calcium and iron.
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  • 29
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    Nutrition & food science 99 (1999), S. 105-107 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The increasingly multidisciplinary nature of food in society has been reflected at South Bank University by the development of undergraduate courses in Food Science and Technology; Food, Nutrition and Health; and Food Choice and Design. The ethos of The Food Programme of courses is outlined and the structure explained. Applications from students from a wide variety of backgrounds are welcome - provided they have a real interest in food.
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    Nutrition & food science 99 (1999), S. 136-139 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper discusses the findings of a market research programme carried out by SMRC ChildWise on behalf of Tetra Pak UK Ltd, examining use of and attitudes to soft drinks among children and mothers. This is a product field of increasing interest to children as they grow, and their appreciation develops from being totally product-focused, to include packaging and then brand considerations. The research examined behaviour across the key drinking occasions that make up the child's day, looking in detail at drinks consumed at school. It is at school that a child's freedom of choice develops, and awareness of peer group influences becomes increasingly important. Children are highly receptive to new brands and flavours, but also demand professional packaging and promotional input for brands to be credible. Younger children appreciate having their own individual carton with a straw but, for older boys, a can is preferred, whilst girls like the practicalities of resealable bottles.
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  • 31
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    Nutrition & food science 99 (1999), S. 81-88 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Describes chromium's importance in nutrition and health, pinpointing its sources in diets and facts about its absorption in humans. Outlines the body's ability to store chromium and the function of the element. Reports on current recommendations of safe and adequate levels of intake. The symptoms of chromium deficiency are discussed as are the toxic effects of too high an intake. Concludes with recommendations for required further research.
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  • 32
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    Nutrition & food science 99 (1999), S. 89-98 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: While there is considerable anecdotal evidence and some research indicating poor nutritional intake and high levels of food wastage in hospitals, there have been no studies relating these issues to the catering system used. The overall purpose of this study was, therefore, to measure food wastage and nutritional intake in selected hospital catering systems. Data were collected from three types of ward (elderly, medical and surgical) in four hospitals (nine wards), two in London and two in Southern England. Three wards used food cooked mainly in the hospital kitchen, six used cook-chill and cook-freeze dishes bought in ready prepared. Five of the wards used a bulk system where food is transported to the ward and plated, in the others, food is plated in the hospital kitchen then transported to the ward. Food sent to the ward, served to patients, and that which remained uneaten or left on the service trolley was weighed for a minimum of 24 hours in each ward; 966 patient-meal-days. This data enabled food wastage and nutritional intake to be calculated. Results indicate that food wastage was lower at the breakfast meal, than the midday and evening meal, 23.10 per cent, 39.99 per cent and 42.35 per cent, respectively; female wastage was higher than male, 33.91 per cent and 27.26 per cent, respectively; wastage was higher where food was plated in wards rather than in the kitchen, 57.75 per cent and 35.28 per cent, respectively; and wastage was higher where food was purchased-in ready prepared, rather than prime cooked in the hospital kitchen. Nutritional intake was calculated for five wards and in all, energy intake was below the recommendations, the highest deficit being 58 per cent. Deficiencies were also noted for other nutrients.
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  • 33
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    Nutrition & food science 99 (1999), S. 99-104 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Zinc and essential fatty acid deficiencies manifest similar syndromes provoking investigations into the mechanisms underlying their metabolic interactions. This article presents a lucid and concise review of literature on the possible connection between these important dietary nutrients as exemplified by the role of zinc in modulating intestinal lipid transport and prostaglandins metabolisms as well as maintenance of membrane structural and functional integrity. Reports of investigations on zinc and essential fatty acid deficiencies, regarding their pleitropic effects on clinically important enzymes, from the laboratory of the authors were also considered.
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  • 34
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    Nutrition & food science 99 (1999), S. 285-287 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Describes coeliac disease with its increased risk of osteoporosis for the patient. Often misdiagnosed or not recognised early, coeliac disease leads to further complications, particularly osteoporosis. Provides eating/nutrition guidelines for coeliac sufferers to avoid osteoporosis in later life.
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  • 35
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    Nutrition & food science 99 (1999), S. 303-306 
    ISSN: 0034-6659
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Highlights the prevalence, causes and health risks of obesity. Then continues by looking at the health incentives of weight loss through commercial weight-reducing diets and the health problems incurred during such a diet in respect of reduced energy intake and reduction of non-starch polysaccharides which can lead to constipation and associated bowel diseases. Tips to increase the intake of non-starch polysaccharides are given in the context of weight-reduction programmes.
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  • 36
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    Pigment & resin technology 28 (1999), S. 279-282 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A lamellar zinc pigment reacts in aqueous alkaline media (e.g. water-borne paints) with the evolution of hydrogen. This corrosion reaction can be inhibited by certain surfactants. The most important structural part of the examined surfactants is the hydrophilic group; only anionic phosphate or phosphonate hydrophilic groups are effective corrosion inhibitors. Surfactants with carboxylate, sulfonate, amphoteric, cationic and non-ionic hydrophilic groups are ineffective. There seems to be also an influence of the hydrophobic group of the surfactants because a partial ester of phosphoric acid with a fluorinated hydrophobic group was the most effective corrosion inhibitor in this study.
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  • 37
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    Pigment & resin technology 28 (1999), S. 283-292 
    ISSN: 0369-9420
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Reports the effects of composition and curing temperature on the film properties of three water reducible enamels prepared from palm stearin alkyds. The properties studied were hardness, flexibility, and adhesion. While all the formulations exhibit excellent adhesion, generally increasing the melamine content and curing temperature can increase the hardness but reduce the resistance to cracking and deformation of the coating. Applies Fourier transform infra-red spectroscopy (FTIR) to the study of the curing reactions. Finds that FTIR is able to identify the predominant cross-linking reactions.
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  • 38
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    Pigment & resin technology 28 (1999), S. 326-330 
    ISSN: 0369-9420
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fillers with different chemical properties were used to study their effects on poly(vinyl acetate) (PVAc) emulsion wood adhesives. The elastoplastic behaviour of the filler treated PVAc emulsions was studied using differential scanning calorimetry and dynamic mechanical thermal analysis. The results show that the glass transition temperature (Tg) of the polymer is not changed significantly by the addition of the fillers, while the tensile modulus is changed together with the hardness and stiffness. The viscosity of the emulsions and their performance on wood surfaces are greatly affected by fillers. Fillers with high oil-absorption capacity increase viscosity to a greater extent. The acidity of the fillers influences the setting time for bonding of the emulsions to wood, and the water resistance, since some chemical reactions can take place during curing.
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  • 39
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    Pigment & resin technology 28 (1999), S. 336-341 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Copolymer resins of peanut skin tannin extract, aldehydes and cashew nut shell liquid were prepared. The resins were blended with bitumen and used in formulating oleoresinous wood varnishes. The film properties of the varnishes were determined and the results showed that the gloss and scratch hardness of the films increased with increase in the quantity of cashew nut shell liquid/tannin-aldehyde resins incorporated. The results also showed that the resins improved the chemical resistance of the varnish films. Varnish compositions containing 50:50 of bitumen and the resins gave films with satisfactory physical and chemical properties. The study showed that it is possible to formulate excellent oleoresinous wood varnishes using blends of bitumen and cashew nut shell liquid/tannin-aldehyde resins.
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  • 40
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    Pigment & resin technology 28 (1999), S. 341-344 
    ISSN: 0369-9420
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Benzamide has been made to undergo Hofmann rearrangement, in the presence of hypochlorite (OCl-) generated in situ, by the electrolysis of sodium chloride solution using platinised titanium anode and graphite cathode. The product is not a pure primary amine, namely aniline; but a polymeric aniline derivative is produced. It has a free amino end; and/or a free amino end is generated during the process of diazotisation. The product has been diazotised and coupled with naphthols to give dyes. The nature of the dyes have been analysed by uv-visible spectral studies. The dyes are slightly different from the dyes prepared from pure aniline.
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  • 41
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Surface of precipitated silica was modified with 3-aminopropyltriethoxysilane. The optimum solvent was selected, in which the silane coupling agent was deposited on silica surface. Basic physicochemical properties of the modified silica were estimated. Methods of evaluating silica surface modification extent were presented, taking advantage first of all of its altered hydrophilicity and of differences in condensation extent of surface silanol groups. The modified silica was used as adsorbent capable of trapping water soluble organic amines.
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  • 42
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    Pigment & resin technology 28 (1999), S. 293-296 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Peanut skin tannin extract-formaldehyde condensates were modified with resorcinol and cashew nut shell liquid. The copolymer resins formed were used as thermosetting and cold-setting exterior grade wood adhesives for Sapele veneer panels. Results obtained showed that, on addition of paraformaldehyde, the resins modified with resorcinol cured at ambient temperatures while those modified with cashew nut shell liquid cured at higher temperatures. The bonded panels developed strength and durability satisfying the requirements of International Standard Specification for phenolic resin adhesives.
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  • 43
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    Pigment & resin technology 28 (1999), S. 331-336 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Traditionally aerospace coatings have been formulated for performance. In an extreme case a faulty coating could contribute to an accident on a catastrophic scale. The demands on aerospace coatings are severe because aircraft have unusual requirements. These requirements are dictated by the environment in which modern aircraft operate, the nature of the structure of the airframe, the way they are painted and the way in which they are used. As a consequence of all this, the paints which have been formulated for aerospace use usually differ from paint used in other industrial areas. It has often meant using ingredients which are regarded as hazardous either to health, such as chromate pigments, or to the environment, such as large quantities of strong solvents. Bearing this in mind, modern formulations have had to evolve, improving performance and taking into account the results of using hazardous ingredients which might affect users of the coatings, innocent bystanders and the environment. Here we consider the conflict between these influences and show the position reached within the industry.
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  • 44
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    Pigment & resin technology 28 (1999), S. 83-88 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aims to prepare some organotin polymers based on N-substituted acrylamide and methylacrylamide acid esters via a new synthetic route. Tri-n-butyltin-?-bromoacrylate monomer (TBTBA) was prepared and polymerisation carried out. Organotin polymers were prepared via the new synthetic route by exchange reactions.
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  • 45
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    Pigment & resin technology 28 (1999), S. 158-161 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article describes the move towards integrated coating systems, where the coating and method of drying or curing are selected to ensure efficient coating processes, while ensuring there is no reduction in the function of the coating itself. It briefly explores various methods of radiation curing and explains how these techniques can be used to meet production requirements and constraints and develop optimum coating solutions. There is also a brief case study, which describes the installation of an infra-red system for pre-heating steel drums in order to speed up a lacquering operation.
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  • 46
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    Pigment & resin technology 28 (1999), S. 143-148 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lignin precipitated from different black liquors wasted from the cooking of rice straw, bagasse and cotton stalks, to produce pulp and paper, can replace phenol by up to 40 per cent in phenol formaldehyde resin. The properties of the resin produced from bagasse lignin formaldehyde are nearly the same as when the resin IS produced from phenol formaldehyde. Replacement of phenol by lignin in phenol formaldehyde resin has an economical effect and reduces the pollution caused by draining black liquor into rivers and streams. The properties of the resin produced from rice straw lignin are lower than resin from bagasse and cotton stalk lignin. The effect of increasing the content of lignin in the resin on the resin properties was studied. The effect of polymerization time and temperature on the resin properties is also clarified. The molecular structure of the lignins used plays an important role on the properties of the phenol lignin formaldehyde produced.
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  • 47
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    Pigment & resin technology 28 (1999), S. 233-237 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article describes the background to the widespread application of infrared radiation as a curing technique for paints and coatings, from its origins in the 1930s to the present day. It explains the mechanism of infrared and elaborates on some of the developments which have been made in the technology, especially in terms of control and response. The benefits of infra-red drying and curing are further illustrated by reference to case studies demonstrating infrared in action.
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    Pigment & resin technology 28 (1999), S. 230-232 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aqueous sodium chloride solution, containing benzamide was electrolysed (pH = 2), using platinised titanium anode and graphite cathode. The filtrate of the reaction mixture (pH = 4.5) was diazotised and coupled with a-naphthol in NaOH and also with ß-naphthol in NaOH. Dyes were produced. These dyes were matched with the standard dyes prepared from aniline. Uv-visible absorption spectral study reveals that the dyes produced are slightly different from the standard dyes. During the course of the reaction benzamide is converted into aniline with a long chain polymeric unit having a free amino end; and/or a polymeric unit which generates a free amino end during the process of diazotisation.
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  • 49
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    Pigment & resin technology 28 (1999), S. 217-222 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In many markets customers judge the quality of products by their surface appearance. The term gloss describes the perception of the shiny appearance of a surface when light is reflected from that surface. Imperfections in a surface cause some of the light to be reflected/scattered at angles away from the specular and this impacts on the visual assessment of appearance. A method has existed for measuring surface reflectance for the last 50 years, but its usefulness has been limited by an inability to equal the human eye in discerning subtleties such as haze or distinctness of reflected images. A portable instrument has now been developed to take measurements about the specular, spaced at less than three minutes of arc. This paper explains how this high resolution supports the introduction of additional indices to define haze, distinctness of image and peak specular reflectance while retaining the definition of "gloss" as described in the standard method ISO2813.
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    Pigment & resin technology 28 (1999), S. 6-14 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Colour preferences have both scientific significance and relevance to manufacturers. Despite claims that these preferences are unsystematic and that saturation and brightness exert more influence on judgements than hue, a substantial body of research suggests that the rank order of preference for hues - blue, red, green, violet, orange, yellow - emerges with some degree of consistency and, in particular, blue is regularly preferred to other hues. Five explanations of this trend are considered: preferences are simply conventional; blue is more neutral and less susceptible to extremes of judgement than other hues; preference for blue is a by-product of more general principles; blue has largely positive associations; blue has an evolutionary significance. It is proposed that further investigation of the connotations of hues will provide insight into the pattern of colour preferences.
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    Pigment & resin technology 28 (1999), S. 26-30 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: UV-curable systems based on the copolymerisation of a typical acrylic resin with low amount of fluorinated monomers (less than 1 per cent w/w) were prepared. The bulk properties of the films were unchanged, while a strong modification of the surface was obtained, depending on the monomer structure, on its concentration and on the type of substrate.
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    Pigment & resin technology 28 (1999), S. 31-33 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Briefly discusses the search for alternatives to CFCs in aerosols for precision cleaning applications. Gives properties of several solvents used in cleaning applications. Considers five key parameters for selecting a solvent for aerosol applications.
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    Pigment & resin technology 28 (1999), S. 75-82 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Discusses the use of piperylene-styrene copolymer (PSC) for polychloroprene adhesive modification. States that PSC significantly improves modified adhesive properties - bond strength, viscosity, high heat resistance, good adhesion to a variety of substrates, compatibility with other adhesive additives. Looks at the advantage of using more environmentally friendly technology for thermoplastic rubber bonding with PSC modified polychloroprene adhesives. Concludes that the new adhesive product can be used for specific combinations of materials and application methods, making it possible to produce cheaper and better products.
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    Pigment & resin technology 28 (1999), S. 212-216 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: New acrylic and allylic resins were prepared by functionalising perfluoropolyethers chains, which assured peculiar surface properties. Blends having a different amount of the acrylic and of the allylic systems were cured in air by UV irradiation. The cure of the films was found dependent on the ratio between the two resins; the concentration for the obtaining of a film fully cured in air was determined. On the basis of this concentration, a macromer functionalised with the right amount of both the acrylic group and the allylic one was synthesised.
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    Pigment & resin technology 28 (1999), S. 15-25 
    ISSN: 0369-9420
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Environmental, economic and market pressures have led to a dramatic increase in the production of recycled paper fibre in recent years and the growing demand for higher quality grades is now focusing attention on the recycling of office wastes. Although the role of chemistry in the recycling process for paper is well established, the introduction of newer inks and adhesives into the recycling chain has added to the existing problems of "getting the chemistry right". This article examines the impact on the deinking of secondary paper fibre resulting from evolving ink technologies, catalysed by developments in the printing processes.
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    Pigment & resin technology 28 (1999), S. 70-74 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hydroxy terminated polyesters were prepared by the reaction of tetrabromophthalic, phthalic, and maleic anhydrides with polyethylene glycol via melt condensation technique. They were characterized by hydroxy number, and intrinsic viscosity measurements. Also, the fire retardancy of polyesters was evaluated as varnish films on plywood strips. The polyesters were reacted with excess toluene -2, 4- diisocyanate to yeild isocyanate terminated prepolymers which were subsequently reacted with 5, 5- dihydroxymethyl-2-trichloro-methyl-1,3- dioxane or ethelene diamine. These polymers were characterized by Ir and 1H NMR spectra, viscosity measurements, and thermogravimetric analysis.
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    Pigment & resin technology 28 (1999), S. 223-229 
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    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article provides a summary of the Health and Safety Executive's (HSE) work over recent years to address occupational exposure to particulates during the manufacture and use of coating powders. It contends, in particular, that many users of coating powders are not controlling exposure to total inhalable particulate (TIP) (i.e. the total inhalable dust in the air from all sources), and that these control issues would exist even if TGIC (triglycidyl isocyanurate) was not being used. TGIC is a curing agent for polyester coating powders which is classified as a Category 2 mutagen. HSE is raising awareness that control of exposure is generally poor whatever powders are being used.
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    Pigment & resin technology 28 (1999), S. 149-157 
    ISSN: 0369-9420
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    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Titanium dioxide pigments have been produced commercially for eight decades. The industry has seen many developments from the humble beginnings with uncoated, impure, anatase pigments to the highly refined TiO2 pigments of today. For the past four decades there have been two commercial routes for making TiO2 pigments - sulphate and chloride. In recent years, significant efforts have been made to produce TiO2 by both routes with greater environmental awareness. To compare the overall impact of TiO2 manufacturing processes on the environment, life-cycle assessments of several process options are described in this paper.
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  • 59
    ISSN: 1476-5535
    Keywords: Keywords: Bacillus subtilis; riboflavin; 3,4-dihydroxy-2-butanone 4-phosphate synthase; GTP cyclohydrolase II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: One of the proteins encoded by the riboflavin operon of Bacillus subtilis, RibA, was identified as the rate limiting enzyme in an industrial riboflavin producing strain. An additional single copy of the ribA gene was introduced into the sacB locus of the riboflavin production strain and was expressed constitutively from the medium strength vegI promoter. This led to improved riboflavin titers and yields of riboflavin on glucose of up to 25%. Both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 39-43 
    ISSN: 1476-5535
    Keywords: Keywords: murein; cell wall hydrolase; phage lysin; thymol-triggered lysis
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    Notes: Effective disruption of Escherichia coli cells is achieved by the intracellularly accumulated recombinant murein hydrolase (Lactobacillus bacteriophage LL-H muramidase) after the addition of 5 mM thymol. Thymol destroys the integrity and electric potential of the cytoplasmic membrane, and as a consequence the muramidase can access and hydrolyze the cell wall murein leading to cell lysis. Lysis occurred within 5 min after the addition of thymol and seemed to be efficient at high culture densities. This lysis method does not require cell harvesting or addition of other cell wall weakening substances or exogenous enzymes. As a cell disruption method, thymol-triggered lysis is as efficient as sonication in the presence of 1% Triton. Furthermore, thymol did not interfere with the purification steps of Mur by expanded bed adsorption chromatography (EBA), suggesting that the lysis method presented here is well suited for large-scale production and purification of intracellular proteins of E. coli.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 52-57 
    ISSN: 1476-5535
    Keywords: Keywords: nitro-PAHs; metabolism; Cunninghamella elegans; biotransformation
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    Notes: Biotransformation of 1-nitrobenzo[e]pyrene (1-nitro-BeP), an environmental pollutant derived from the nitration of a non-carcinogen, benzo[e]pyrene, was studied using the fungus Cunninghamella elegans ATCC 36112. After 72 h incubation, 89% of 1-nitro-[3H]BeP added had been metabolized to two major metabolites. These metabolites were separated by reversed-phase high performance liquid chromatography and identified by 1H NMR, UV-visible, and mass spectral techniques as 1-nitro-6-benzo[e]pyrenylsulfate and 1-nitrobenzo[e]pyrene 6-O-β-glucopyranoside. Comparison of the fungal metabolism patterns of 1-nitro-BeP and BeP indicates that the nitro group at the C-1 position of BeP altered the regioselectivity of metabolism.
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  • 62
    ISSN: 1476-5535
    Keywords: Keywords: recombinant Bacillus subtilis; riboflavin produced by fermentation; down-stream processing; analytics; registration
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A novel process for riboflavin production using a recombinant Bacillus subtilis strain has been developed. Here we describe a down-stream processing procedure to obtain riboflavin qualities having a minimal content of 96% (‘feed-grade’) and 98% (‘food/pharma-grade’) riboflavin, respectively. Compared to riboflavin produced by chemical synthesis, products with improved chemical purity were obtained. All compounds representing more than 0.1% of the final products were identified. Feed-grade riboflavin material ex fermentation contained small amounts of amino acids and amino sugars and the biosynthetic riboflavin precursor dimethyl-ribityl-lumazine. All other side products found were derived from riboflavin, resulted from the purification procedure and were also found in riboflavin obtained by chemical synthesis. The Bacillus-produced riboflavin does not contain DNA. The data presented here were used to obtain product approval for the commercial application in the USA, Japan and the UK.
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  • 63
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 80-87 
    ISSN: 1476-5535
    Keywords: Keywords: airborne bacteria; phospholipid fatty acids; human health
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Exposure to airborne biocontaminants may result in a multitude of health effects and is related to a pronounced increase in adult-onset asthma. Established culture-based procedures for quantifying microbial biomass from airborne environments have severe limitations. Assay of the phospholipid fatty acid (PLFA) components of airborne microorganisms provides a quantitative method to define biomass, community composition and nutritional/physiological activity of the microbial community. By collecting airborne particulate matter from a high volume via filtration, we collected sufficient biomass for quantitative PLFA analysis. Comparing high (filtration) and low (impaction) volume air sampling techniques at 26 locations within the Eastern United States, we determined that PLFA analysis provided a viable alternative to the established but flawed culture-based techniques for measuring airborne microbial biomass and community composition. Compared to the PLFA analysis, the culture techniques underestimated the actual viable airborne biomass present by between one to three orders of magnitude. A case study of a manufacturing plant at which there had been complaints regarding the indoor air quality is presented. Phospholipid fatty acid characterization of the biomass enabled contamination point source determination. In comparison with samples taken outdoors, increases in the relative proportion of trans PLFA, reflecting shifts in the physiological status of viable airborne Gram-negative bacteria, were detected in the indoor air samples at a majority of sampling sites.
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  • 64
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 96-99 
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    Keywords: Keywords: Streptomyces sp; α-amylase; thermostability; structure-function; dimerisation
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The amylolytic system of Streptomyces sp IMD 2679 is composed of three α-amylases, amylase I, II and III, with temperature maxima of 60, 60–65 and 65°C, respectively. Although each α-amylase displayed higher stability in the pH range 6.0–8.5 than at pH 5.0–5.5, differences in their thermostabilities were more evident as the pH increased from pH 6.0 to 8.5. There was a 14-min difference in half-lives between amylase III, the most thermostable enzyme and amylase II at pH 6.0, and a 46-min difference in the half-lives of amylase III and the least thermostable enzyme, amylase I at pH 6.5. In addition, the α-amylases underwent a pH-dependent monomer-dimer transformation. Increased thermostability of the α-amylases was reflected in the variable contents of amino acids (Arg, His, Ser) responsible for electrostatic interactions, and in the levels of aliphatic and bulky hydrophobic amino acids. There was a two-fold reduction in Cys levels in amylase III relative to amylase I and II.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 121-126 
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    Keywords: Keywords: biodegradation; phenol; Pseudomonas putida; immobilized
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    Notes: Alginate concentrations between 2 and 4% had little effect on the degradation rate of phenol by alginate-immobilized Pseudomonas putida. Ten-degree shifts from 25°C resulted in approximately 30% slower degradation. Maximal degradation rates were favored at pH 5.5–6.0. The response of degradation rate to increased air flow in the bubble column used was almost linear and an optimal higher than 16 vol vol−1 was indicated, although free cells appeared in the reaction medium above 12 vol vol−1. When the initial phenol concentration was raised, degradation rate was not significantly affected until levels higher than 1200 mg ml−1 where performance was markedly reduced. Increasing the ratio of total bead volume to medium volume gave progressively smaller increases in degradation rate. At a medium volume to total bead volume ratio of 5:1, the maximum degradation rate was 250 mg L−1 h−1.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 160-163 
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    Keywords: Keywords: β-glucosidase; cellobiase; cellobiose-hydrolysis; Aureobasidium
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: β-Glucosidase hydrolyzing cellobiose was extracted from Aureobasidium sp ATCC 20524 and purified to homogeneity. The molecular mass was estimated to be about 331 kDa. The enzyme contained 26.5% (w/w) carbohydrate. The optimum pH and temperature for the enzyme reaction were pH 4 and 80°C, respectively. The enzyme was stable at a wide range of pH, 2.2–9.8, after 3 h and at 75°C for 15 min. The kinetic parameters were determined. The enzyme was relatively stable against typical organic enzyme inhibitors. The enzyme also hydrolyzed gentiobiose, p-nitrophenyl-β-glucoside and salicin.
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  • 67
    ISSN: 1476-5535
    Keywords: Keywords: umami; L-glutamic acid, glutaminase; Cryptococcus
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    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An anamorphic basidiomycetous yeast, which produced a salt-tolerant and thermostable glutaminase, was isolated from soil in Japan and classified in the genus Cryptococcus. Its substrate specificity suggests that this enzyme is an L-glutaminase asparaginase (EC 3.5.1.38). The strain, G60, resembles Cryptococcus laurentii in the taxonomic criteria traditionally employed for yeasts, however it can be distinguished as a separate species based on DNA–DNA reassociation experiments and sequence analysis of the large sub-unit rDNA. Phenotypically, the isolate can be differentiated from C. laurentii by the inability to utilize arbutin as a sole source of carbon. Based on sequence analysis, the strain is related to a group of hymenomycetous yeasts including Bulleromyces albus, Bullera unica, C. laurentii and C. skinneri. The strain, which is formally described as Cryptococcus nodaensis, is industrially important for the formation of the umami taste during production of proteolytic seasonings.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 216-224 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 167-175 
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    Keywords: Keywords: engineered biofilms; biocorrosion; sulfate-reducing bacteria
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    Notes: To identify novel, less-toxic compounds capable of inhibiting sulfate-reducing bacteria (SRB), Desulfovibrio vulgaris and Desulfovibrio gigas in suspension cultures were exposed to several antimicrobial peptides. The bacterial peptide antimicrobials gramicidin S, gramicidin D, and polymyxin B as well as the cationic peptides indolicidin and bactenecin from bovine neutrophils decreased the viability of both SRB by 90% after a 1-h exposure at concentrations of 25–100 μg ml−1. To reduce corrosion by inhibiting SRB in biofilms, the genes for indolicidin and bactenecin were expressed in Bacillus subtilisBE1500 and B. subtilis WB600 under the control of the constitutive alkaline protease (apr) promoter, and the antimicrobials were secreted into the culture medium using the apr signal sequence. Bactenecin was also synthesized and expressed as a fusion to the pro-region of barnase from Bacillus amyloliquefaciens. Concentrated culture supernatants of B. subtilis BE1500 expressing bactenecin at 3 μg ml−1 decreased the viability of Escherichia coli BK6 by 90% and the reference SRB D. vulgaris by 83% in suspension cultures. B. subtilis BE1500 and B. subtilis WB600 expressing bactenecin in biofilms also inhibited the SRB-induced corrosion of 304 stainless steel six to 12-fold in continuous reactors as evidenced by the lack of change in the impedance spectra (resistance polarization) upon addition of SRB and by the reduction in hydrogen sulfide and iron sulfide in batch fermentations with mild steel. A 36-fold decrease in the population of D. vulgaris in a B. subtilis BE1500 biofilm expressing bactenecin was also observed. This is the first report of an antimicrobial produced in a biofilm for in vivo applications and represents the first application of a beneficial, genetically-engineered biofilm for combating corrosion.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 225-240 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 259-269 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 288-297 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 315-322 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 349-360 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 394-399 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 418-427 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 449-459 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 518-525 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 540-550 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 551-563 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 428-429 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 187-187 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 206-215 
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 600-607 
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    Keywords: Keywords: endoglucanase; ethanol; Klebsiella; Erwinia; lignocellulose; biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Klebsiella oxytoca P2 was developed as a biocatalyst for the simultaneous saccharification and fermentation (SSF) of cellulose by chromosomally integrating Zymomonas mobilis genes (pdc, adhB) encoding the ethanol pathway. This strain contains the native ability to transport and metabolize cellobiose, eliminating the need to supplement with β-glucosidase during SSF. To increase the utility of this biocatalyst, we have now chromosomally integrated the celZ gene encoding the primary endoglucanase from Erwinia chrysanthemi. This gene was expressed at high levels by replacing the native promoter with a surrogate promoter derived from Z. mobilis DNA. With the addition of out genes encoding the type II protein secretion system from E. chrysanthemi, over half of the active endoglucanase (EGZ) was secreted into the extracellular environment. The two most active strains, SZ2(pCPP2006) and SZ6(pCPP2006), produced approximately 24 000 IU L−1 of CMCase activity, equivalent to 5% of total cellular protein. Recombinant EGZ partially depolymerized acid-swollen cellulose and allowed the production of small amounts of ethanol by SZ6(pCPP2006) without the addition of fungal cellulase. However, additional endoglucanase activities will be required to complete the depolymerization of cellulose into small soluble products which can be efficiently metabolized to ethanol.
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  • 85
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    Keywords: Keywords: biotin production; E. coli bio operon; Agrobacterium/Rhizobium HK4; limiting growth conditions
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    Notes: E. coli biotin (bio) operon was modified to improve biotin production by host cells: (a) the divergently transcribed wild-type bio operon was re-organized into one transcriptional unit; (b) the wild-type bio promoter was replaced with a strong artificial (tac) promoter; (c) a potential stem loop structure between bioD and bioA was removed; and (d) the wild-type bioB ribosomal binding site (RBS) was replaced with an artificial RBS that resulted in improved bioB expression. The effects of the modifications on the bio operon were studied in E. coli by measuring biotin and dethiobiotin production, and bio gene expression with mini-cells and two-dimensional polyacrylamide gel electrophoresis. The modified E. coli bio operon was introduced into a broad host-range plasmid and used to transform Agrobacterium/Rhizobium HK4, which then produced 110 mg L−1 of biotin in a 2-L fermenter, growing on a defined medium with diaminononanoic acid as the starting material. Biotin production was not growth-phase dependent in this strain, and the rate of production remained high under limiting (maintenance) and zero growth conditions.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 627-632 
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    Keywords: Keywords: fermentation; maltose metabolism; yeast; baking; distilling; brewing; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Saccharomyces cerevisiae are unable to maintain high rates of fermentation during transition from catabolism of hexoses to maltose. This phenomenon, termed ‘maltose lag’, presents problems for the baking, brewing and distilling industries, which rely on yeast catabolism of mixtures of hexoses and maltose. Maltose utilisation requires the presence of maltose permease and α-glucosidase (maltase), encoded by MAL genes. Synthesis of these is induced by maltose and repressed by glucose. One strain of baker’s yeast used in this work exhibited a marked maltose lag, whereas a second strain exhibited a shorter lag during conversion from hexose to maltose metabolism. The extent of the lag was linked to the levels of maltose permease and maltase in cells at the time of inoculation into mixed sugar medium. This view is supported by results showing that pulsing yeast with maltose to induce expression of MAL genes prior to inoculation into mixed sugar medium, enhanced sugar fermentation. Maltose pulsing of yeasts could therefore be useful for enhancing some fermentations relevant to baking and other yeast industries.
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    Journal of industrial microbiology and biotechnology 22 (1999), S. 622-626 
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    Keywords: Keywords: amyloglucosidase; glucoamylase; raw starch hydrolysis; Aspergillus; solid state fermentation
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    Notes: Aspergillus sp GP-21 produced a raw-starch digesting amyloglucosidase which showed optimum activity at 65°C and pH 5.0–5.5. At 50°C the enzyme converted about 40% of raw corn starch to glucose within 48 h. Enzyme production was studied in solid state fermentation using wheat bran. Productivity was affected by the level of moisture, incubation temperature and the presence or absence of supplements. Maximum enzyme production was observed at a moisture level of 75% and at 30°C. Enzyme production was stimulated by supplementing wheat bran with 0.25% proteose peptone, 1% trace mineral solution, 0.01% CaCl2 and 0.01% MgSO4.
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 641-646 
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    Keywords: Keywords: Streptomyces hygroscopicus; biocontrol; fungi; turf; thatch
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    Notes: Disease prevention is a current practice used to minimize fungal diseases of turfgrasses in lawns and golf greens. Prevention is accomplished through fungicide applications, and by periodic thatch removal. During the development of a microbial biodethatch product utilizing the lignocellulose-degrading Streptomyces hygroscopicus strains YCED9 and WYE53, we demonstrated using in vitro plate antagonism bioassays that both strains are antagonists of various turfgrass fungal pathogens. These activities were present when the cultures were growing on thatch, as demonstrated by antifungal antagonism bioassays with culture filtrates. Experiments conducted using a growth chamber demonstrated that a bio-dethatch formulation containing spores of strains YCED9 and WYE53 in a zeolite carrier, provided protection for Kentucky bluegrass seedlings against turfgrass pathogens, including Pythium ultimum, Fusarium oxysporum, Rhizoctonia solani, Sclerotinia homeocarpa, Gaeumannomyces graminis and Microdochium nivale. Results showed that by integrating the use of the S. hygroscopicus YCED9/WYE53 bio-dethatch formulation into routine turf management practices, it should be possible to both minimize thatch build-up while also controlling fungal turfgrass diseases by way of the antifungal biocontrol activity of these strains. This in turn would help control fungal pathogens in turfgrass while minimizing the need for routine chemical fungicide applications.
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 682-685 
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    Keywords: Keywords: Trichoderma; xylan; xylanase; characterization
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    Notes: A new xylanase (XYL2) was purified from solid-state cultures of Trichoderma harzianum strain C by ultrafiltration and gel filtration. SDS-PAGE of the xylanase showed an apparent homogeneity and molecular weight of 18 kDa. It had the highest activity at pH 5.0 and 45°C and was stable at 50°C and pH 5.0 up to 4 h xylanase. XYL2 had a low K m with insoluble oat spelt xylan as substrate. Compared to the amino acid composition of xylanases from Trichoderma spp, xylanase XYL2 presented a high content of glutamate/glutamine, phenylalanine and cysteine, and a low content of serine. Xylanase XYL2 improved the delignification and selectivity of unbleached hardwood kraft pulp.
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 691-696 
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    Keywords: Keywords: Haematococcus pluvialis; mixotrophic culture; light irradiance; astaxanthin production; kinetic model
    Source: Springer Online Journal Archives 1860-2000
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    Notes: High cell density cultivation of Haematococcus pluvialis for astaxanthin production was carried out in batch and fed-batch modes in 3.7-L bioreactors with stepwise increased light intensity control mode. A high cell density of 2.65 g L−1 (batch culture) or 2.74 g L−1 (fed-batch culture) was obtained, and total astaxanthin production in the fed-batch culture (64.36 mg L−1) was about 20.5% higher than in the batch culture (53.43 mg L−1). An unstructured kinetic model to describe the microalga culture system including cell growth, astaxanthin formation, as well as sodium acetate consumption was proposed. Good agreement was found between the model predictions and experimental data. The models demonstrated that the optimal light intensity for mixotrophic growth of H. pluvialis in batch or fed-batch cultures in a 3.7-L bioreactor was 90–360 μmol m−2 s−1, and that the stepwise increased light intensity mode could be replaced by a constant light intensity mode.
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 709-712 
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    Keywords: Keywords: Streptomyces thermonitrificans; thermophilic Streptomyces sp; extracellular DNase; enzyme production; metal ions
    Source: Springer Online Journal Archives 1860-2000
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    Notes: A thermophilic bacterial strain, Streptomyces thermonitrificans, produced high levels of extracellular deoxyribonuclease (DNase) when grown on NBG medium (containing 1% peptone, 0.3% beef extract, 1% glucose and 0.5% NaCl). Maximum DNase activity (140 U ml−1) was obtained, in 24 h, when the culture was grown on modified NBG medium (containing 1.3% beef extract, 1% glucose, 0.5% NaCl and 50 μM Mn2+ at 45°C. The crude enzyme showed higher activity on native DNA than on sonicated and heat denatured DNA. Moreover, addition of Mn2+ in the assay mixture resulted in a significant stimulation (10–15 fold) of the enzyme activity.
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 701-708 
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    Keywords: Keywords: ethanol; recombinant; E. coli KO11; lignocellulosic; chemostat; stability
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Differing claims regarding the stability of the recombinant ethanologen E. coli KO11 are addressed here in batch and chemostat culture. In repeat batch culture, the organism was stable on glucose, mannose, xylose and galactose for at least three serial transfers, even in the absence of a selective antibiotic. Chemostat cultures on glucose were remarkably stable, but on mannose, xylose and a xylose/glucose mixture, they progressively lost their hyperethanologenicity. On xylose, the loss was irreversible, indicating genetic instability. The loss of hyperethanologenicity was accompanied by the production of high concentrations of acetic acid and by increasing biomass yields, suggesting that the higher ATP yield associated with acetate production may foster the growth of acetate-producing revertant strains. Plate counts on high chloramphenicol-containing medium, whether directly, or following preliminary growth on non-selective medium, were not a reliable indicator of high ethanologenicity during chemostat culture. In batch culture, the organism appeared to retain its promise for ethanol production from lignocellulosics and concerns that antibiotics may need to be included in all media appear unfounded.
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  • 93
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 79-85 
    ISSN: 1476-5535
    Keywords: Keywords: Ca-alginate entrapped lactobacilli; dehydration; water content; protective solutes; survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Lactobacillus helveticus CNRZ 303 entrapped in Ca-alginate gel beads was investigated for improved survival and stability during fluidized-bed drying, storage and rehydration. Addition of protective solutes was very important. Studies of the conditions showed that inactivation of entrapped L. helveticus started when the water content exceeded 0.3–0.4 g H2O (g dry wt)−1 for adonitol, glycerol and reconstituted non fat milk solids (NFSM). With Ringer’s solution (control) and betaine, the fall in viability was evident above 1 g H2O (g dry wt)−1. Drying down to 0.2 g H2O (g dry wt)−1 required the removal of 98.5–98.9% of the water. The best survival rate with the least injured cells among survivors was experienced with adonitol and NFMS, respectively, 71% and 57% (compared to the initial) immediately after dehydration. Adonitol and NFMS were also best for survival during storage. The highest cell recovery was obtained by rehydrating the cells in cheese whey permeate between 20–30°C done at pH 6.0–7.0, satisfying the demands for cell survival, repair and slow swelling (adaptions).
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  • 94
    ISSN: 1476-5535
    Keywords: Keywords: GM-CSF; LIF; baculovirus system; transfer vector; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A fusion gene coding human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) cDNAs was inserted into the transfer vector pSXIVVI+ X3 with the control of Syn and XIV promoters. The Sf9 cells (Spodoptera frugiperda) were co-transfected with the recombinant plasmid and TnNPV DNA (Trichoplusia ni nuclear polyhedrosis virus DNA). The fusion protein recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF) and leukemia inhibitory factor (LIF) could be synthesized in cells infected with recombinant virus at a level of about 23% of their total cellular protein. Activity analysis of the fusion protein in infected cells revealed that it exhibited the dual activities of GM-CSF and LIF. Western blot analysis of the expressed fusion protein in infected larvae showed that the virus-mediated fusion protein, with a molecular weight of ∼35 kDa, is confirmed with immunoreactivity.
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  • 95
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 138-142 
    ISSN: 1476-5535
    Keywords: Keywords: biocatalysis; 2-aminomuconate; 2-aminophenol; Pseudomonas; dioxygenase; dehydrogenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: 2-Aminomuconate is an intermediate in the oxidative metabolism of tryptophan in mammals. The compound is not commercially available, and studies of its metabolism have been prevented by the lack of a chemical synthesis and the instability of the molecule. We report here the formation of 2-aminomuconate from 2-aminophenol by the coupled action of 2-aminophenol 1,6-dioxygenase and 2-aminomuconic semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45, and isolation of the product by anion exchange chromatography. The overall procedure was completed within 3 h with a yield of 62%. The availability of the dicarboxyl α-amino acid provides the basis for investigation of the physiological function of 2-aminomuconate in the neuropathologically significant oxidative metabolism of tryptophan.
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  • 96
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 143-148 
    ISSN: 1476-5535
    Keywords: Keywords: curdlan production; optimal pH profile; batch process
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: We sought an optimal pH profile to maximize curdlan production in a batch fermentation of Agrobacterium species. The optimal pH profile was calculated using a gradient iteration algorithm based on the minimum principle of Pontryagin. The model equations describing cell growth and curdlan production were developed as functions of pH, sucrose concentration, and ammonium concentration, since the specific rates of cell growth and curdlan production were highly influenced by those parameters. The pH profile provided the strategy to shift the culture pH from the optimal growth condition (pH 7.0) to the optimal production one (pH 5.5) at the time of ammonium exhaustion. By applying the optimal pH profile in the batch process, we obtained significant improvement in curdlan production (64 g L−1) compared to that of constant pH operation (36 g L−1).
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  • 97
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 178-187 
    ISSN: 1476-5535
    Keywords: Keywords: actinomycete diversity; phylogeny; rRNA; tropical rainforest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Five thousand actinomycetes were isolated from soil samples collected from rainforests in Singapore and the generic identities of these isolates were determined by using a procedure that combined morphological, chemotaxonomic and 16S rDNA sequence-based phylogenetic analyses. Actinomycetes belonging to a total of 36 genera were identified. The most abundant isolates are members of Streptomyces, Micromonospora, Actinoplanes, Actinomadura, Nonomuria, Nocardia and Streptosporangium. By phylogenetic analysis of 16S rDNA sequences of our isolates together with those of known actinomycete species, we also evaluated the species diversity of several genera including Streptomyces, Micromonospora, Nonomuria, and Actinomadura. We found that: first, the tropical isolates are present in most clades represented by known species; and second, many tropical isolates form new clades distant from the known species, indicating the presence of unidentified taxa at both species and genus levels. Based on these results, we conclude that actinomycete diversity in the tropical rainforest is very great and should represent an excellent source for discovery of novel bioactive compounds.
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  • 98
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 223-229 
    ISSN: 1476-5535
    Keywords: Keywords: biological control; postharvest diseases; yeasts; fruits; biofungicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The yeasts Rhodotorula glutinis (LS-11), Cryptococcus laurentii (LS-28), Candida famata (21-D) and Pichia guilliermondii (29-A) and the yeast-like fungus Aureobasidium pullulans (LS-30), previously selected and characterized for mechanisms of action and antagonistic activity against postharvest pathogens in small and large-scale experiments, were used in this study in order to assess interrelationships among the main factors (antagonist, host fruit and fungal pathogen) involved in biological control of postharvest diseases. The antagonists were evaluated for their inhibitory activity (IA) against six common postharvest fungal pathogens on six different host fruits. Artificially wounded fruits were first inoculated with the antagonist and 2 h later with the pathogen; subsequently they were kept at 20°C for 4–6 days. The IA of each antagonist was evaluated and data were submitted to factorial analysis of variance. The populations of antagonists were also monitored on wounded and unwounded fruits kept at 20°C for 7 days. Each factor examined (antagonist, host fruit and fungal pathogen) as well as their interactions significantly affected the IA. However, among the antagonists, isolates LS-28 and LS-30 were only slightly affected by both host and pathogen, showing a wide range of activity, whereas isolate LS-11 had a variable IA. All the antagonists rapidly colonized the wounds, while their population remained substantially unchanged on unwounded fruits. These results suggest that in order to select yeasts with a broad spectrum of action, more suitable for commercial development, it would be advantageous to perform preliminary assays against several pathogens and in particular on different fruit species.
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  • 99
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    Journal of industrial microbiology and biotechnology 23 (1999), S. 442-445 
    ISSN: 1476-5535
    Keywords: Keywords: Sphingomonas paucimobilis GS-1 exopolysaccharide; rheological properties; biopolymer; drilling fluid; oil exploration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Analysis of an exopolysaccharide of Sphingomonas paucimobilis GS-1 (EPS/GS-1) with respect to its rheological properties, cross-linking ability with chrome alum and performance test at 75 ± 5°C revealed its strong suspending ability, shear thinning property, and thixotrophic nature which are required to impart desirable rheology to drilling mud. The organism fulfilled all the specified requirements and its properties were superior to those of currently-used XC polymer (a xanthan product) for oil drilling applications. However, EPS/GS-1 was unstable in the presence of bentonite at 100 ± 5°C during performance tests, in contrast to XC polymer.
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  • 100
    ISSN: 1476-5535
    Keywords: Keywords: Bacillus; siderophores; antimicrobial; biocides; white water; paper- and boardmachine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The antagonistic potential of nonpathogenic Staphylococcus strains against Bacillus subtilis wild and type strains were studied under conditions simulating a paper- and board-machine environment. The antimicrobial activity was measured by growth inhibition in an automated turbidimeter. The antagonistic potential was compared with that of generally used chemical biocides in a paper- and board-machine environment. The siderophore-containing extracts of Staphylococcus strains significantly inhibited vegetative growth of B. subtilis and delayed the germination of spores both in synthetic and in white-water media. The mill strains were more resistant than type strain against Staphylococcus siderophores and against chemical biocides. The Staphylococcus siderophore-containing extracts did not interfere with the bacteriostatic effect of chemical biocides, but no synergy was detected. The results indicate the potential for application of Staphylococcus siderophore-containing extracts as biocontrol agents in paper- and boardmachine environment.
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