ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Journals
  • Articles  (192)
  • Agrobacterium
  • transformation
  • 1995-1999  (180)
  • 1960-1964
  • 1955-1959  (12)
Collection
  • Journals
  • Articles  (192)
Publisher
Years
Year
  • 1
    ISSN: 1572-8773
    Keywords: NMR and EPR spectroscopy ; speciation ; transformation ; vanadate ; vanadyl ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three strains of Saccharomyces cerevisiae, SC-1, DBVPG 6173 and DBVPG 6037, were studied for vanadate resistance in complex Sabouraud medium since they did not thrive in different minimal media (yeast nitrogen base with and without amino acids). The strain SC-1 was resistant up to 16 mm of vanadate, whereas the strains DBVPG 6173 and DBVPG 6037 were inhibited by 8 mm and 4 mm vanadate, respectively. The vanadate resistance in strain SC-1 was constitutive and due to the reduction of this oxyanion to vanadyl, which was detected by EPR spectroscopy and visible spectroscopy. The transformation of vanadate to vanadyl took place during the exponential growth phase; 10 mm of vanadate was reduced to vanadyl outside the cells since the oxyanion was not detected in the cell biomass and only a negligible concentration of vanadyl (25 nmoles mg cells dry weight) was found in the biomass. The other two vanadate-sensitive yeast strains only accumulated vanadate and did not reduce the oxyanion to vanadyl.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Catalysis letters 49 (1997), S. 121-128 
    ISSN: 1572-879X
    Keywords: cresols ; transformation ; isomerization ; disproportionation ; zeolites HY
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The transformation of m- and o-cresol has been investigated on a series of HY zeolites with Si/Al ratio in the range of 4.5 to 55, at 380°C and atmospheric pressure. It was found that the zeolite activity increased but stability decreased with NAl for both m- and o-cresol. For Si/Al = 4.5 the zeolite activity was similar for both reactants, but as Si/Al ratio increased, o-cresol became relatively more reactive than m-cresol, and for all Si/Al ratios the catalysts were more stable in the m-cresol transformation. Both m- and o-cresol were converted mainly through two parallel reactions - isomerization and disproportionation. The disproportionation reaction was predominant, and more important in the case of o-cresol. The isomerization/disproportionation selectivity increased for m-cresol and decreased for o-cresol with NAl. Catalyst deactivation favoured disproportionation in the case of o-cresol and isomerization in the case of m-cresol, except for o-cresol conversion on HY(4.5), where both reactions were equally affected. Isomerization selectivity for m-cresol (p/o) was found to be higher than the equilibrium value and to increase with catalyst deactivation; while for o-cresol, the isomerization ratio (m/p) did not change with time on stream for HY(4.5) and HY(16.6) and decreased for HY(55). Disproportionation selectivity (ph/∑x) was equal to unity independently of Si/Al ratio or catalyst deactivation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Georgian mathematical journal 2 (1995), S. 1-8 
    ISSN: 1572-9176
    Keywords: 34K05 ; 34K15 ; Differential equation ; delay argument ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The aim of this paper is to find the class of continuous pointwise transformations (as general as possible) in the framework of which Kummer's transformationz(t)=g(t)y(h(t)) represents the most general pointwise transformation converting every linear homogeneous differential equation of thenth order into an equation of the same type. Further, some forms of these equations having certain subspaces of solutions are considered.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Zeitschrift für angewandte Mathematik und Physik 47 (1996), S. 821-836 
    ISSN: 1420-9039
    Keywords: Stream functions ; vector potential ; transformation ; vortex flows
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Abstract Introducing a vector potential, that is based on a pair of stream functions, and a velocity potential, antisymmetric equations for the stream functions are derived with the help of a variational principle. It is found that the equations are in a suitable form to investigate flows with helical symmetry, and, for example, to connect upstream axisymmetric flows with downstream helical flows. The special case of a transition from an upstream solid-body vortex to a downstream helical flow is investigated in detail. Furthermore, the stream-function equations are particularly useful to investigate general small-amplitude inertia waves on vortex flows. Time-dependent helical flows that are time-independent in a suitably rotating frame of reference can also be discussed with the proposed method.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 5
    Electronic Resource
    Electronic Resource
    Springer
    International journal of value-based management 10 (1997), S. 9-29 
    ISSN: 1572-8528
    Keywords: business ethics ; Jewish ; religious ; community ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper examines the method of Jewish business ethics. MichaelWalzer, in his work, Interpretation and Social Criticism (1987), suggeststhree common and important approaches to moral philosophy. He labels thesethe path of discovery, the path of invention, and the path ofinterpretation. The first part of this paper argues that Jewish businessethics is best thought of in terms of interpretation. Without question, thereligious ethicist immediately recognizes Walzer‘s metaphor of the moralworld as a ’home occupied by a single family over many generations... ‘as his own. Ethical arguments from a Jewish perspective must of necessityhave a ’lived-in quality‘ and always make reference to and are based on the’memory-laden objects and artifacts.‘ The second part of the paper exploressome of the implications of Jewish business ethics as interpretation.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-0789
    Keywords: Cyanobacteria ; Soil inoculation N ; transformation ; Inorganic N ; Easily oxidizable N ; Hydrolysable N ; Non-hydrolysable N ; Wetland rice Farmyard manure ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field experiment was conducted with wetland rice (Oryza sativa cv. IR-36) in a sandy clay loam soil (Entisol) to study the effect of inoculation with a soil-based mixed culture of four diazotrophic cyanobacteria,Aulosira fertilissima, Nostoc muscorum, N. commune andAnabaena spp., on the N-flux in inorganic NH4 ++NO3 −+ NO2 −), easily oxidizable, hydrolysable and non-hydrolysable forms of N in soil during vegetative growth periods of the crop. Effects on grain and straw yield and N uptake by the crop were estimated. The effects of applying urea N and N as organic sources, viz.Sesbania aculeata, Neem (Azardirachta indica) cake and FYM, each at the rate of 40 kg N ha−1, to the soil were also evaluated. Inoculation significantly increased the release of inorganic N, evidenced by its increased concentrations either in soil or in soil solution. However, such increases rarely exceeded even 4% of total N gained in different froms in the soil system by inoculation during the vegetative growth stages of the rice plant, when the nutritional requirement of the plants is at a maximum. Most of the N2 fixed by cyanobacteria remained in the soil as the hydrolysable form (about 85%) during this period. Inoculation caused an insignificant increase in grain (8%) and straw (11%) yield, which was, however, accompanied by a significant increase in N uptake by the grain (30%) and an increase in total uptake of 15.3 kg N ha 1. Such beneficial effects of inoculation varied in magnitude with the application of organic sources, with farmyard manure (FYM) being the most effective. Application of urea N, on the other hand, markedly reduced such an effect.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology reporter 17 (1999), S. 323-331 
    ISSN: 1572-9818
    Keywords: Agrobacterium ; modular vector ; transformation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat (cv Chinese Spring) tissues were transformed using Agrobacterium tumefasciens and a new plasmid modular vector, pMVTBP. We constructed pMVTBP with unique restriction sites connecting (1) the CaMV 35S promoter, (2) a Kozak sequence, (3) the FLAG epitope, (4) the (His)6 epitope, (5) a coding region (for wheat TATA Binding Protein, wTBP) and (6) the CaMV 35S 3′UTR. This vector thus allows easy exchange of different regulatory or coding sequences. Explants of either germinating mature seeds, or immature embryos, were induced to callus for up to two weeks, treated with virulence-induced bacteria for one hour, then regenerated into plantlets. Transient expression of a GUS reporter gene, assayed at about one week, occurred in 10–12% of calluses. Expression of the FLAG-tagged wTBP was also detected, by immunostaining. Stable expression, by selective growth on geneticin, and by GUS expression at about six weeks, occurred in 1–2% of calluses, quite comparable to that achieved by other methods.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 8
    ISSN: 1572-9818
    Keywords: Agrobacterium ; Populus ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In recent years, Populus species have acquired an important place in basic and applied research of woody plants. The practical role of Populus species in world forestry and their importance to research as a woody-plant model have led to increasing interest in tissue-culture and molecular techniques, as well as the development of transformation procedures for this genus. A simple technical procedure is described here step-by-step, for the first time, as a routine method for transforming Populus tremula using a disarmed Agrobacterium tumefaciens hypervirulent strain. The procedure begins with the inoculation of stem explants with bacterial suspension, followed by a short period of co-cultivation on a highly regenerative medium. Transformed shoots are selected on regeneration medium containing antibiotics and the presence of the inserted target genes is checked using a rapid and efficient PCR test. Selected shoots are transferred to a rooting medium, under the same selection pressure, and propagated via stem cuttings. Selected plants can be hardened and transferred to the green-house within 4 months of inoculation. The method has proven efficient for several gene constructs, selection on Kan or Hyg, and three different Agrobacterium strains.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology reporter 15 (1997), S. 209-215 
    ISSN: 1572-9818
    Keywords: selectable marker ; cereals ; leaf assay ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A very simple leaf assay is described that rapidly and reliably identifies transgenic plants expressing the hygromycin resistance gene, hph or the phosphinothricin resistance gene, bar. Leaf tips were cut from plants propagated either in the glasshouse or in tissue culture and the cut surface embedded in solid medium containing the appropriate selective agent. Non-transgenic barley or rice leaf tips had noticeable symptoms of either bleaching or necrosis after three days on the medium and were completely bleached or necrotic after one week. Transgenic leaf tips remained green and healthy over this period. This gave unambiguous discrimination between transgenic and non-transgenic plants. The leaf assay was also effective for dicot plants tested (tobacco and peas).
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Antonie van Leeuwenhoek 73 (1998), S. 117-126 
    ISSN: 1572-9699
    Keywords: riboplasmids ; encapsidation ; pseudovirions ; selfish plasmids ; replicons ; ribozyme ; Agrobacterium ; Rhizobium ; grapevines ; L-tartrate ; lignin ; methoxyphenols ; satellite viruses ; opines ; crown gall ; T-DNA ; origin of replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies on the origin and evolution of plasmids may provide valuable insights on the promiscuous nature of DNA. The first examples of the selfish nature of nucleic acids are exemplified by primordial oligoribonucleotides which evolved into primitive replicons. The propagation of these molecules were likely patterned after the current viral RNA ribozymes, which have been recently shown to possess RNA synthesizing and template mediated polymerizing capabilities in the absence of proteins. The parasitic nature of nucleic acids is depicted by satellite nucleic acid molecules associated with viruses. The satellites of adenovirus and tobacco ringspot virus serve as established examples: they contain no open reading frames. Comparative analysis of the replication origins of virions and plasmids show them to be conserved, originating from the simplest autocatalytic replicon to highly complex and evolved plasmids, replicating by a rolling circle mechanism. The eventual association of proteins with nucleic acids provided added efficiency and protective advantages for molecular perpetuation. The promiscuous and selfish nature of plasmids is demonstrated by their ability to genetically engineer their host so that the host cell is best able to cope and survive in hostile environments. Survival of the host ensures survival of the plasmid. Sequestering of genes by plasmids occurs when the environmental conditions negatively affect the host. The sequestering mechanism is fundamental and forms the outreach mechanisms to generate and propagate macromolecules of increasing size when necessary for survival. The level of sophistication of plasmids increases with the addition of new genes such as those that allow the host to occupy a specific environment normally inhospitable to the host cell. The vast range of plasmid types which have obtained genes interchangeably reflect the levels of sophistication achieved by these macromolecules. The Ti plasmid in Agrobacterium tumefaciens and the pSym and accessory plasmids in Rhizobium illustrate the level of complexity attained by replicons.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...