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  • Articles  (6)
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  • RAPD
  • Springer  (6)
  • Nature Publishing Group
  • 1995-1999  (6)
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  • Process Engineering, Biotechnology, Nutrition Technology  (6)
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  • Articles  (6)
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  • Springer  (6)
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  • 1
    Electronic Resource
    Electronic Resource
    Intraspecific diversity in the fungal speciesChaunopycnis alba: Implications for microbial screening programs (1996)
    Springer
    Journal of industrial microbiology and biotechnology 17 (1996), S. 359-372 
    Details
    ISSN: 1476-5535
    Keywords: intraspecific variation ; RAPD ; HPLC profile ; temperature requirements ; cyclosporin synthetase gene ; creativity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Intraspecific variation among 84 isolates of the anamorphic fungusChaunopycnis alba from 26 different geographical locations was analyzed by investigating optimal growth temperatures, differences in the production of secondary metabolites and presence or absence of the cyclosporin synthetase gene. The genetic diversity was assessed using random amplified polymorphic DNA (RAPD). Analysis of these data showed high genetic, metabolic and physiological diversity within this species. Isolates from the Antarctic represented the most homogeneous group withinC. alba and together with isolates from the Arctic these polar strains differed from alpine, temperate and tropical strains by low optimal growth temperatures and by low production of secondary metabolites. Isolates from tropical climes were characterized by high optimal growth temperatures and by the production of comparatively diverse metabolite spectra. Most of the isolates that were similar in the combination of their physiological and metabolic characters were also genetically related. Isolates from different geographical origins did not show many similarities, with the exception of the cyclosporin A-producing isolates, and large diversity could be observed even within a single habitat. This leads us to the suggestion that for pharmaceutical screening programs samples should be collected from a diversity of different geographical and climatic locations. For the selection of strains for screening the RAPD assay seems to be the most powerful tool. It reflected the highest intraspecific diversity and the results corresponded well with the other characteristics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01574767
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  • 2
    Electronic Resource
    Electronic Resource
    Application of DNA amplification fingerprinting (DAF) to mixed culture bioreactors (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 10-16 
    Details
    ISSN: 1476-5535
    Keywords: DNA fingerprinting ; RAPD ; Community structure ; Polymerase chain reaction (PCR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The use of DNA amplification fingerprinting (DAF) as a tool for monitoring mixed microbial populations in bioreactors was evaluated. Short (8-mer or 10-mer) oligonucleotides were used to prime DNA extracts from various biological reactors during polymerase chain reaction (PCR) amplification. The reactors examined in this study included two sets of anaerobic stirred tank continuous flow bioreactors. One set of anaerobic reactors was operated under methanogenic conditions and one set was operated under sulfate-reducing conditions. The anaerobic reactor communities in the methanol-fed reactors showed extensive DAF homology. DAF was also applied to a fixed-film azo dye degrading reactor to examine the degree of uniformity of colonization of the substratum in representative regions of the reactor. This method is a quick and relatively inexpensive means of monitoring microbial community structure during biological processes. Since no cultivation of the sample is involved, the genetic profile of the community is not biased by outgrowth conditions. DAF profiles may be useful for comparisons of population changes over time or of bench-scale vs pilot-scale reactors but not adequate for assessing community diversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570059
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  • 3
    Electronic Resource
    Electronic Resource
    Variation of RAPD-fingerprint patterns using different DNA-extraction methods with Gram-positive bacteria (1995)
    Springer
    World journal of microbiology and biotechnology 11 (1995), S. 238-239 
    Details
    ISSN: 1573-0972
    Keywords: DNA extraction ; micro technique ; RAPD ; Staphylococcus ; Streptococcus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The effect of the DNA-extraction method used on fingerprint patterns of RAPD was studied usingStaphylococcus epidermidis andStreptococcus faecalis. The three methods tested (Chelex, microwave and phenol/chloroform) led to significantly different RAPD patterns. The microwave technique generated reproducible patterns and seems the most suitable for RAPD analysis of Gram-positive bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00704659
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  • 4
    Electronic Resource
    Electronic Resource
    Detection of a specifically amplified DNAfragment in Brucella abortus by arbitrarilyprimed polymerase chain reaction (1998)
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 415-420 
    Details
    ISSN: 1573-0972
    Keywords: Arbitrary primers ; Brucella abortus ; Brucella melitensis ; polymerase chain reaction ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Randomly amplified polymorphic DNA (RAPD) profiles of Brucella and non-Brucella DNA were established after polymerase chain reaction (PCR) amplification. Five arbitrary oligonucleotide primers were screened to generate Brucella-specific DNA fingerprints. The arbitrary primer OPB-01 (5′-GTTTCGCTCC-3′) produced DNA bands specific to Brucella. Amplification conditions must be optimized for reproductibility. Accordingly, we optimized and established the conditions, which included Mg2+, enzyme (DNA polymerase), primer, template and deoxyribonucleoside triphosphate (dNTP) concentrations as well as the optimum number of thermal cycles to produce OPB-01 directed Brucella DNA fingerprints. The optimized RAPD method can produce a 1.3 kb DNA fragment specific to Brucella. This DNA fragment was common to eight biovars of B. abortus and one biovar of B. melitensis. The fragment was not detected in genetically related species such as Ochrobactrum anthropi and other non-Brucella organisms associated with farm animals. We anticipate the use of this fragment as a possible probe for the detection of Brucella organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008877630986
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  • 5
    Electronic Resource
    Electronic Resource
    Use of randomly amplified polymorphic DNA analysis to differentiate isolates of Vibrio parahaemolyticus from cockles (Anadara granosa) (1998)
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 895-901 
    Details
    ISSN: 1573-0972
    Keywords: V. parahaemolyticus ; plasmid ; RAPD ; cockles (Anadara granosa)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A total of 35 Kanagawa-negative strains of Vibrio parahaemolyticus isolated from cockles (Anadara granosa) were investigated by randomly amplified polymorphic DNA fingerprinting with three primers and their plasmid profiles. Eighteen strains carried small plasmid(s) of 2.4 to 7.3kb that enabled the V. parahaemolyticus to be grouped into eight plasmid patterns. The three primers generated polymorphisms in all 35 strains of V. parahaemolyticus tested, producing bands ranging from 0.25 to 3.9kb. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested, and that cockles in the study area are populated by genetically polymorphic strains of V. parahaemolyticus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008841514368
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  • 6
    Electronic Resource
    Electronic Resource
    The use of an arbitrarily primed PCR product for the specific detection of Brucella (1999)
    Springer
    World journal of microbiology and biotechnology 15 (1999), S. 381-385 
    Details
    ISSN: 1573-0972
    Keywords: Brucella abortus ; Brucella melitensis ; polymerase chain reaction ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A 1.3 kb Brucella-specific DNA fragment produced through the use of arbitrarily primed polymerase chain reaction (AP-PCR) was tested for its specificity by DNA–DNA hybridization to Brucella and non-Brucella bacteria. The digoxigenin (DIG)-labelled 1.3 kb DNA fragment hybridized with Brucella abortus and Brucella melitensis but did not hybridize with other non-Brucella bacteria tested. The sensitivity of the reaction was determined; as little as 150 fg DNA or 30 Brucella cells could be detected. The specificity and sensitivity of the 1.3 kb DNA fragment combined with the simplicity and speed of the technique suggest the potential of this fragment as a DNA probe for the quick and reliable detection of Brucella organisms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008975616874
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