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  • climate change  (49)
  • gene expression  (49)
  • Springer  (98)
  • American Geophysical Union
  • American Physical Society
  • 2000-2004  (98)
  • 1965-1969
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  • Springer  (98)
  • American Geophysical Union
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of paleolimnology 23 (2000), S. 49-56 
    ISSN: 1573-0417
    Keywords: Trichoptera ; caddisflies ; late glacial ; Allerød ; Younger Dryas ; early Holocene ; Kråkenes ; palaeolimnology ; climate change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Fossil Trichoptera (caddisfly) remains have been identified and quantitatively recorded in the late-glacial and early-Holocene sediments from Kråkenes Lake, western Norway. The sediment sequence was deposited between 12,300 and 8850 14C BP, covering the Allerød, Younger Dryas, and early-Holocene periods. The first Trichoptera were recorded at 12,000 14C BP, and during the Allerod a diverse assemblage of Limnephilidae taxa developed in the lake. By about 11,400 14C BP the relatively thermophilous Polycentropus flavomaculatus and Limnephilus rhombicus were present, suggesting that the summer water temperature was at least 17 °C. This temperature fell by 5-8 °C at the start of the Younger Dryas, and the thermophilous taxa were replaced within 20-40 14C yrs by Apatania spp., including the arctic-alpine A. zonella, suggesting a maximum summer water temperature of 10-12 °C. The Trichoptera assemblage was impoverished in numbers and in diversity over the next 200 yrs as the severe conditions of the Younger Dryas developed. As soon as temperatures rose and glacial meltwater and silt input ended about 700 14C yrs later, the resident Apatania assemblage expanded immediately, within 10 yrs. About 130 yrs later, thermophilous taxa replaced Apatania, and a much more diverse assemblage than in the Allerod occupied the varied habitats made available by the development of the Holocene lake ecosystem. The 130 yr delay may have been caused by a gradual temperature increase crossing a critical threshold, or by the time taken for thermophilous taxa to migrate from their Younger Dryas refugia.
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  • 2
    ISSN: 1573-0417
    Keywords: Lake Baikal ; diatoms ; biogenic silica ; Eemian ; climate change ; Siberia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The discussion on climatic instability observed in Greenland ice cores during the Eemian period (substage 5e) resulted in discovery of a pronounced mid-Eemian cooling event. We report that the mid-Eemian cooling is found for the first time in the biogenic silica climatic record and microfossil abundance record of Lake Baikal. Timing of this event in Lake Baikal correlates well with timing of the European pollen records and marine sedimentary records. The presence of the mid-Eemian cooling signal in the Lake Baikal record suggests a much closer link between Asian climate influenced by strong pressure fields over the vast land masses and the climate-controlling processes in the North Atlantic during interglacial periods, than what was generally believed. Furthermore, the Lake Baikal record suggests that after the mid-Eemian cooling, the climatic conditions returned close to the warmth of the 5e optimum and thus argues that the warm conditions of the last interglacial persisted in Siberia throughout 5e, and did not end with the mid-Eemian cooling as suggested by several published marine records.
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  • 3
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    Journal of paleolimnology 24 (2000), S. 1-13 
    ISSN: 1573-0417
    Keywords: Arctic ; Holocene ; paleohydrology ; paleolimnology ; climate change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract Although paleoclimatic research in the Arctic has most often focused on variations in temperature, the Arctic has also experienced changes in hydrologic balance. Changes in Arctic precipitation and evaporation rates affects soils, permafrost, lakes, wetlands, rivers, ice and vegetation. Changes in Arctic soils, permafrost, runoff, and vegetation can influence global climate by changing atmospheric methane and carbon dioxide concentrations, thermohaline circulation, and high latitude albedo. Documenting past variations in Arctic hydrological conditions is important for understanding Arctic climate and the potential response and role of the Arctic in regards to future climate change. Methods for reconstructing past changes in Arctic hydrology from the stratigraphic, isotopic, geochemical and fossil records of lake sediments are being developed, refined and applied in a number of regions. These records suggest that hydrological variations in the Arctic have been regionally asynchronous, reflecting the impacts of different forcing factors including orbitally controlled insolation changes, changes in geography related to coastal emergence, ocean currents, sea ice extent, and atmospheric circulation. Despite considerable progress, much work remains to be done on the development of paleohydrological proxies and their application to the Arctic.
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  • 4
    ISSN: 1573-0417
    Keywords: diatoms ; climate change ; temperature ; pH ; transfer functions ; lake sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The relationships between diatoms (Bacillariophyceae) in surface sediments of lakes and summer air temperature, pH and total organic carbon concentration (TOC) were explored along a steep climatic gradient in northern Sweden to provide a tool to infer past climate conditions from sediment cores. The study sites are in an area with low human impact and range from boreal forest to alpine tundra. Canonical correspondence analysis (CCA) constrained to mean July air temperature and pH clearly showed that diatom community composition was different between lakes situated in conifer-, mountain birch- and alpine-vegetation zones. As a consequence, diatoms and multivariate ordination methods can be used to infer past changes in treeline position and dominant forest type. Quantitative inference models were developed to estimate mean July air temperature, pH and TOC from sedimentary diatom assemblages using weighted averaging (WA) and weighted averaging partial least squares (WA-PLS) regression. Relationships between diatoms and mean July air temperature were independent of lake-water pH, TOC, alkalinity and maximum depth. The results demonstrated that diatoms in lake sediments can provide useful and independent quantitative information for estimating past changes in mean July air temperature (R2 jack = 0.62, RMSEP = 0.86 °C; R2 and root mean squared error of prediction (RMSEP) based on jack-knifing), pH (R2 jack = 0.61, RMSEP = 0.30) and TOC (R2 jack = 0.49, RMSEP = 1.33 mg l-1). The paper focuses mainly on the relationship between diatom community composition and mean July air temperature, but the relationships to pH and TOC are also discussed.
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  • 5
    ISSN: 1573-0417
    Keywords: carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.
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  • 6
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    Nutrient cycling in agroecosystems 58 (2000), S. 179-199 
    ISSN: 1573-0867
    Keywords: climate change ; databases ; GIS ; methane ; rice ; soils ; weather
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract As part of a series of papers describing the use of a simulation model to extrapolate experimental measurements of methane (CH4) emissions from rice fields in Asia and to evaluate the large-scale effect of various mitigation strategies, the collation and derivation of the spatial databases used are described. Daily weather data, including solar radiation, minimum and maximum temperatures, and rainfall were collated from 46 weather stations from the five countries in the study, namely China, India, Indonesia, Philippines, and Thailand. Quantitative soil data relevant to the input requirements of the model were derived by combining data from the World Inventory of Soil Emissions (WISE) database, the ISIS database, and the FAO Digital Soil Map of the World (FAO-DSMW). These data included soil pH; organic carbon content; sand, silt, and clay fractions; and iron content for top and subsoil layers, and average values of bulk density and available water capacity for the whole profile. Data on the areas allocated to irrigated, rainfed, upland, and deepwater rice at the province or district level were derived from the Huke & Huke (1997) database developed at IRRI. Using a geographical information system (GIS), a series of georeferenced data sets on climate, soils, and land use were derived for each country, at the province or district level. A summary of the soil-related derived databases is presented and their applicationn for use in global change modeling discussed.
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  • 7
    ISSN: 1570-0267
    Keywords: cDNA ; PCR cDNA ; TaqMan Analysis ; gene expression ; Pearson's correlation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Comparative gene expression studies are often limited by low availability of tissue and poor quality of extractable mRNA. Collective PCR amplification of minute quantities of mRNA has great potential for overcoming these limitations. However, there remains significant concern about the effects of amplification on the absolute and relative abundance of individual mRNAs that could complicate subsequent gene expression studies. To address this problem, we systematically compared the relative abundance of many specific mRNAs from complex cDNA preparations (from tissue and cultured cells) both before and after amplification by PCR. Our results demonstrated that, as expected, the absolute abundance of different mRNAs in a cDNA library is altered in an unpredictable manner by PCR amplification. However, we found that the concentration ratios of specific mRNAs among different cDNA preparations were routinely well conserved after PCR amplification. Thus, for the purpose of comparative expression studies for specific mRNAs in two (or more) complex cDNAs, PCR-amplified cDNA is equally useful as unamplified cDNA. These results provide a rigorous experimental validation and offer a theoretical treatment to support the utility of PCR amplified cDNA for differential gene expression studies. We conclude that the inherent difficulties in performing differential screening studies such as gene chip and array analyses on limited amounts of biological materials can be overcome by a PCR amplification step without compromising data quality.
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  • 8
    ISSN: 1573-4919
    Keywords: myosin heavy chain ; gene expression ; hypertrophy ; dexamethasone ; promoter function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Cardiac hypertrophy has been observed in newborn infants treated with dexamethasone (DEX). This study was undertaken to examine whether DEX-induced hypertrophy in newborn rats is associated with redistribution of cardiac myosin heavy chain (MHC) isoforms and if so, the effects involve transcriptional regulation. Newborn rats were injected with either DEX (1 mg/kg/day; s.c.) or equivalent volume normal saline for 1, 3, 5, 7 or 9 days. Hypertrophy was quantified by heart dry/wet wt ratios, heart/body wt ratios, and total protein content of the myocardium. Changes in the expression of cardiac MHC mRNA were characterized by northern blot and slot blot analyses, using isoform specific probes for a- and β-MHC genes. DEX effect on α-MHC gene transcription was analyzed by transiently transfecting various α-MHC promoter/CAT reporter constructs into primary cultures of cardiac myocytes derived from one day old rat pups. DEX administration into newborn rats produced significant cardiac hypertrophy ranging from 23% at day 1 to 59% at 9 days. The hypertrophy was accompanied by immediate increase (83%) in steady state level of the α-MHC mRNA within one day and a maximum increase (148%) at 7 days of treatment. The steady state level of β-MHC mRNA declined by 25% at day 1 and a maximum decrease of 54% at day 7 of DEX treatment. The changes in MHC mRNA were also reflected in their protein levels as determined by V1 and V3 isozyme analysis. DEX treatment of primary cultures of cardiomyocytes following transfection with a-MHC promoter/CAT reporter constructs resulted in increased CAT expression in a dose dependent manner. The minimum α-MHC gene sequences responding to DEX treatment were located between the -200 to -74-bp region of the gene, resulting in 2-fold and 6-fold activation of CAT reporter after 0.05 and 0.1 mM doses of DEX, respectively. Our data indicate that DEX induced cardiac hypertrophy in newborn rats is accompanied by increased expression of α-MHC and decreased expression of β-MHC. The α-MHC effects are mediated in part through transcriptional mechanisms.
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  • 9
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    Molecular and cellular biochemistry 212 (2000), S. 5-9 
    ISSN: 1573-4919
    Keywords: transcriptional regulation ; gene expression ; coactivator ; repressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The CREB-CREM transcription factors are the main gene regulatory effectors of the cAMP signaling pathway. The investigations of this family of transcription factors had a profound impact on the understanding of signaling-induced gene transcription. Here we discuss some key aspects of the underlying biology, review transcriptional activation by CREB proteins through transcription cofactors and present novel insights into the context- and position-specific function of CREB on complex genes.
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  • 10
    ISSN: 1573-4919
    Keywords: AP-1 ; cobalt chloride ; gene expression ; heme oxygenase ; oxidative stress ; sodium arsenite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Previously, chick heme oxygenase-1 (cHO-1) gene was cloned by us and two regions important for induction by sodium arsenite were identified. These two regions were found to contain consensus sequences of an AP-1 (-1580 to -1573) and a MRE/cMyc complex (-52 to -41). In the current study, the roles of these two elements in mediating the sodium arsenite or cobalt chloride dependent induction of cHO-1 were investigated further. DNA binding studies and site-directed mutagenesis studies indicated that both the AP-1 and MRE/cMyc elements are important for the sodium arsenite induction, while cobalt chloride induction involves only the AP-1 element. Electrophoretic mobility shift assays showed that nuclear proteins binding to the AP-1 element was increased by both sodium arsenite or cobalt chloride treatment, whereas the binding of proteins to the MRE/cMyc element showed a high basal expression in untreated cells and the binding activity was only slightly increased by sodium arsenite treatment. Site-directed mutagenesis studies showed that, to completely abolish sodium arsenite induction, both the AP-1 and MRE/cMyc elements must be mutated; mutation of either element alone resulted in only a partial effect. In contrast, a single mutation at AP-1 element was sufficient to reduce the cobalt chloride induction almost completely. The MRE/cMyc complex plays a major role in the basal level expression, and shares some similarities to the upstream stimulatory factor element (USF) identified in the promoter regions of mammalian HO-1 genes and other stress regulated genes. Because sodium arsenite is known to cause oxidative stress and because activation of AP-1 proteins has been shown to be a key step in the oxidative stress response pathway, we also explored the possibility that the induction of the cHO-1 gene by sodium arsenite is mediated through oxidative stress pathway(s) by activation of AP-1 proteins. We found that pretreatment with antioxidants (N-acetyl cysteine or quercetin) reduced the induction of the endogenous cHO-1 message or cHO-1 reporter construct activities induced by sodium arsenite or cobalt chloride. These antioxidants also reduced the protein binding activities to the AP-1 element in the electrophoretic mobility shift assays. In summary, induction of the cHO-1 gene by sodium arsenite or cobalt chloride is mediated by activation of the AP-1 element located at -1,573 to -1,580 of the 5′ UTR.
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  • 11
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    Molecular and cellular biochemistry 205 (2000), S. 1-11 
    ISSN: 1573-4919
    Keywords: kidney ; ischemia-reperfusion injury ; free radicals ; reactive oxygen species ; gene expression ; antioxidant enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Reactive oxygen species (ROS; O2-, H2O2, and OH·), normal by-products of cellular metabolic processes, are kept in control by antioxidant enzymes, such as catalase, glutathione peroxidase (GPX) and superoxide dismutases (SODs). To understand the role of antioxidant enzymatic defenses against ROS injury following ischemia-reperfusion, we examined the effect on kidney exposed to varying periods (30, 60 or 90 min) of ischemia followed by different periods of reperfusion. The enzymatic activities and protein levels of catalase, GPX, CuZnSOD and MnSOD were relatively unaffected at 30 min of ischemia followed by 0, 2 or 24 h reperfusion. However, 60 or 90 min of ischemia followed by 0, 2 or 24 h of reperfusion resulted in a decrease in activities and protein levels which paralleled the duration of ischemic injury. MnSOD activity tended to recover towards normal during reperfusion. Examination of the mRNA levels of these antioxidant enzymes demonstrated a severe decrease in mRNA levels of catalase and GPX at a time point of minimal ischemic injury (30 min of ischemia followed by reperfusion) suggesting that loss of mRNA of catalase and GPX may be the first markers of alterations in cellular redox in ischemia-reperfusion injury. Greater loss of mRNA for catalase, GPX and CuZnSOD were observed following longer periods (60 or 90 min) of ischemia. The mRNA for MnSOD was upregulated at all time points of ischemia-reperfusion injury. Actually, the greater decrease in mRNAs for catalase, GPX and CuZnSOD in the acute phase (within 24 h) subsequently showed a further decrease in these enzyme activities in the subacute phase (72 or 120 h after ischemia). These enzyme activities in the 30 min ischemia group, but not in the 90 min group, already showed tendencies for normalization at 120 h after ischemia. To understand the molecular basis of the loss of mRNA of these antioxidant enzymes during ischemia-reperfusion injury, we examined the rate of transcription by nuclear run-on assays. The similar rates of transcription in control and kidney exposed to ischemia-reperfusion indicates that the loss of mRNA for catalase, GPX and CuZnSOD are possibly due to the increased rate of turnover of their mRNAs. These studies suggest that expression of antioxidant genes during ischemia-reperfusion are not coordinately expressed and the differential loss of antioxidant enzymes may be the contributing factor(s) towards the heterogeneous renal tissue damage as a result of ischemia-reperfusion induced oxidative stress.
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  • 12
    ISSN: 1573-4919
    Keywords: prostaglandin ; cyclooxygenase ; transcriptional regulation ; gene expression ; promotor activation ; transcription ; endothelial cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Interleukin-1β (IL-1) is a potent inducer of cyclooxygenase-2 (COX-2) and prostaglandin biosynthesis in many types of cells, yet little is known about the molecular mechanisms regulating IL-1 mediated prostanoid biosynthesis in the endothelium of the microvasculature. Therefore, we examined the cis- and trans-acting factors regulating IL-1-induced COX-2 expression in the human microvascular endothelial cell line, HMEC-1. IL-1 enhanced steady state levels of COX-2 protein and mRNA synthesis by ≈ 2-fold which preceded a 2-fold increase in PGFα biosynthesis. Expression of a series of COX-2 promoter-luciferase constructs in IL-1 treated HMEC-1 cells revealed that the 'full length' (-1432/+59 bp) promoter was 10 times more active than the SV-40 promoter/enhancer and that it could be further activated by IL-1. Surprisingly however, all except for the shortest COX-2 promoter construct retained the ability to respond to IL-1 and luciferase activity driven by -191/+59 bp COX-2 promoter was as responsive to IL-1 as the full-length promoter. Moreover, site-directed promoter mutagenesis and electophoretic mobility shift assays (EMSA) indicate that the combinatorial actions of AP2, NF-IL6, and CRE elements are critical for both constitutive and IL-1-inducible COX-2 promoter activity. Understanding the mechanism(s) regulating COX-2 gene expression and prostaglandin biosynthesis in the microvasculature has important implications with regard to inflammation and angiogenesis in vivo.
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  • 13
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    Molecular and cellular biochemistry 203 (2000), S. 163-167 
    ISSN: 1573-4919
    Keywords: thymosin β-4 ; gene expression ; chloramphenicol acetyltransferase ; NIH3T3 cells ; interferon response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Expression of the gene coding for thymosin β-4 (Tβ-4), the major G-actin sequestering peptide in the cell, is regulated mainly at the level of transcription. In this study, we examined the nucleotide sequence of the 5′-flanking region (from - 2202 to - 881) of the mouse Tβ-4 gene, and demonstrated that the DNA fragment from -278 to +410 of this gene was capable of directing the expression of a chloramphenicol acetyltransferase reporter gene in NIH3T3 cells. However, expression of the reporter gene in cells cannot be induced by interferon-a treatment even though a rapid activation of endogenous Tβ-4 gene by this cytokine was observed. These results suggest that the projected interferon-stimulated response element (ISRE) might reside in other parts of the mouse Tβ-4 gene.
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  • 14
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    Molecular and cellular biochemistry 211 (2000), S. 103-110 
    ISSN: 1573-4919
    Keywords: thioacetamide ; glutathione-S-transferase ; rat liver ; transcription ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of thioacetamide (TA), an hepatotoxic and hepatocarcinogenic compound, on the expression and activity of the cytosolic enzyme glutathione-S-transferase (GST) was studied in rat liver. Four h following the administration of 14C-labeled thioacetamide (10 mg/Kg), several subunits of GST were found to be radioactively labeled. A single sublethal dose of TA (250 mg/Kg) decreased by three-fold the expression of classα GST at 24-48 h of treatment, but did not significantly affect the transcription of class μ GST. The activity of the enzyme toward 1-chloro-2,4-dinitrobenzene was mildly inhibited (66% of the control) by a 24 h TA treatment and gradually increased thereafter. It is proposed that the covalent binding of TA or its derivative to the GST subunits does not affect the activity of the enzyme. Nevertheless, GST activity inhibition is due to the deleterious effect of TA on GST transcription.
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  • 15
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    Integrated assessment 1 (2000), S. 21-36 
    ISSN: 1573-1545
    Keywords: integrated assessment ; climate change ; regional sustainability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Cohen et al. [16] suggest that in order to explore ways to bring climate change (CC) and sustainable development (SD) research together, it is necessary to develop more heuristic tools that can involve resource users and other stakeholders. In this respect, this paper focuses on methodological development in research to study climate change impacts and regional sustainable development (RSD). It starts with an introduction of an integrated land assessment framework (ILAF) which is part of the integrated phase of the Mackenzie Basin Impact Study (MBIS) in Canada. The paper then provides some articulation on how the integrated approach was applied in the Mackenzie Basin to show implications of climate change for RSD.
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  • 16
    ISSN: 1573-1545
    Keywords: participatory integrated assessment ; climate change ; low energy society
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Within the CLEAR project a new approach to integrated assessment modelling has been developed for the participatory integrated assessment of regional climate change involving citizens' focus groups. The climate change decision problem was structured by focusing separately on climate impacts and mitigation options. The attempt was made to link the different scales of the problem from the individual to the global level. The abstract topic of climate change was related to options on the level of a citizen's individual lifestyle. The option of a low energy society was emphasised in order to embed the climate change decision problem in a wider range of societal concerns. Special emphasis was given to the characterisation and communication of uncertainties. The chosen approach allows different kinds of uncertainties in one framework to be addressed. The paper concludes with a summary of the experience made, and recommendations for the use of models in participatory integrated assessments.
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  • 17
    ISSN: 1573-1545
    Keywords: CLEAR ; natural climate variability ; climate change ; atmosphere ; ocean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Long-term variability in the North Atlantic Oscillation (NAO) and the Atlantic thermohaline ocean circulation (THC) are both shaping the European climate on time scales of decades and longer. Possible linear and non-linear changes in the characteristics of these natural climate modes due to global warming are an important source of uncertainty in long-term regional projections of future climate changes.
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  • 18
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    Integrated assessment 1 (2000), S. 307-320 
    ISSN: 1573-1545
    Keywords: climate change ; ecological impact assessment ; alpine and subalpine belts ; plant distribution ; statistical modeling ; local scale ; GIS ; GLM ; Swiss Alps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The potential ecological impact of ongoing climate change has been much discussed. High mountain ecosystems were identified early on as potentially very sensitive areas. Scenarios of upward species movement and vegetation shift are commonly discussed in the literature. Mountains being characteristically conic in shape, impact scenarios usually assume that a smaller surface area will be available as species move up. However, as the frequency distribution of additional physiographic factors (e.g., slope angle) changes with increasing elevation (e.g., with few gentle slopes available at higher elevation), species migrating upslope may encounter increasingly unsuitable conditions. As a result, many species could suffer severe reduction of their habitat surface, which could in turn affect patterns of biodiversity. In this paper, results from static plant distribution modeling are used to derive climate change impact scenarios in a high mountain environment. Models are adjusted with presence/absence of species. Environmental predictors used are: annual mean air temperature, slope, indices of topographic position, geology, rock cover, modeled permafrost and several indices of solar radiation and snow cover duration. Potential Habitat Distribution maps were drawn for 62 higher plant species, from which three separate climate change impact scenarios were derived. These scenarios show a great range of response, depending on the species and the degree of warming. Alpine species would be at greatest risk of local extinction, whereas species with a large elevation range would run the lowest risk. Limitations of the models and scenarios are further discussed.
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  • 19
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    Molecular and cellular biochemistry 209 (2000), S. 125-129 
    ISSN: 1573-4919
    Keywords: apolipoprotein E ; apolipoprotein A-I ; gene expression ; transgenic mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The levels of plasma apolipoprotein (apo) E, an anti-atherogenic protein involved in mammalian cholesterol transport, were found to be 2-3 fold lower in mice over-expressing human apoA-I gene. ApoE is mainly associated with VLDL and HDL-size particles, but in mice the majority of the apoE is associated with the HDL particles. Over-expression of the human apoA-I in mice increases the levels of human apoA-I-rich HDL particles by displacing mouse apoA-I from HDL. This results in lowering of plasma levels of mouse apoA-I. Since plasma levels of apoE also decreased in the apoA-I transgenic mice, the mechanism of apoE lowering was investigated. Although plasma levels of apoE decreased by 2-3 fold, apoB levels remained unchanged. As expected, the plasma levels of human apoA-I were almost 5-fold higher in the apoAI-Tg mice compared to mouse apoA-I in WT mice. If the over-expression of human apoA-I caused displacement of apoE from the HDL, the levels of hepatic apoE mRNA should remain the same in WT and the apoAI-Tg mice. However, the measurements of apoE mRNA in the liver showed 3-fold decreases of apoE mRNA in apoAI-Tg mice as compared to WT mice, suggesting that the decreased apoE mRNA expression, but not the displacement of the apoE from HDL, resulted in the lowering of plasma apoE in apoAI-Tg mice. As expected, the levels of hepatic apoA-I mRNA (transgene) were 5-fold higher in the apoAI-Tg mice. ApoE synthesis measured in hepatocytes also showed lower synthesis of apoE in the apoAI-Tg mice. These studies suggest that the integration of human apoA-I transgene in mouse genome occurred at a site that affected apoE gene expression. Identification of this locus may provide further understanding of the apoE gene expression.
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  • 20
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    Molecular and cellular biochemistry 212 (2000), S. 29-34 
    ISSN: 1573-4919
    Keywords: cAMP ; transcription factor-decoy oligonucleotides ; CRE ; Ap-1 ; p53 ; tumor growth ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.
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  • 21
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    Molecular and cellular biochemistry 212 (2000), S. 73-79 
    ISSN: 1573-4919
    Keywords: adrenergic receptors ; renin-angiotensin system (RAS) ; gene expression ; kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To investigate the molecular mechanism(s) of action of catecholamines on the expression of the angiotensinogen (ANG) gene in kidney proximal tubular cells, we used opossum kidney (OK) cells with a fusion gene containing the 5′-flanking regulatory sequence of the rat ANG gene fused with a human growth hormone (hGH) gene as a reporter, pOGH (rANG N-1498/+18), permanently integrated into their genomes. The level of expression of the ANG-GH fusion gene was quantified by the amount of immunoreactive-hGH (IR-hGH) secreted into the medium. The addition of norepinephrine (NE), isoproterenol (a β1/β2-adrenergic receptor (AR) agonist) and iodoclonidine (an α2-AR agonist) stimulated the expression of the ANG-GH fusion gene in a dose-dependent manner, whereas the addition of epinephrine and phenylephrine (α1-AR agonist) had no effect. The stimulatory effect of NE was blocked by the presence of propranolol (β-AR blocker), atenolol (β1-AR blocker), yohimbine (α2-AR blocker), Rp-cAMP (an inhibitor of cAMP-dependent protein kinase AI & AII) and staurosporine (an inhibitor of protein kinase C), but was not blocked by ICI 118, 551 (β2-AR blocker) and prazosin (α1-AR blocker). The addition of a combination of isoproterenol and iodoclonidine or a combination of 8-Bromo-cAMP (8-Br-cAMP) and phorbol 12-myristate (PMA) synergistically stimulated the expression of the ANG-GH fusion gene as compared to the addition of isoproterenol, iodoclonidine, 8-Br-cAMP or PMA alone. Furthermore, the addition of NE, 8-Br-cAMP or PMA stimulated the expression of pOGH (rANG N-806/-779/-53/+18), a fusion gene containing the putative cAMP responsive element (CRE, ANG N-806/-779) upstream of the ANG promoter (ANG N-53/+18) in OK cells, but had no effect on the expression of fusion genes containing the mutant of the CRE. Gel mobility shift assays revealed that the ANG-CRE binds with the DNA-binding domain (bZIP 254-327) of the cAMP-responsive binding protein (CREB). The binding of the labeled ANG-CRE to CREB (bZIP254-327) was displaced by unlabeled ANG-CRE and the CRE of the somatostatin gene but not by the mutants of the ANG-CRE. Finally, NE stimulated the phosphorylation of CREB in OK cells. These studies demonstrate that the molecular mechanism(s) of NE action on the expression of the ANG gene in OK cells may be mediated via both the PKA and PKC signalling pathways and via the phosphorylation of CREB. The phosphorylated CREB then interacts with the CRE in the 5′-flanking region of the ANG gene and subsequently stimulates the gene expression.
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  • 22
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    Molecular and cellular biochemistry 212 (2000), S. 135-142 
    ISSN: 1573-4919
    Keywords: gene expression ; catecholamines ; angiotensin II ; heart failure ; myosin ; hypertension ; eprosartan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Pressure overload of the heart is associated with a perturbed gene expression of the cardiomyocyte leading to an impaired pump function. The ensuing neuro-endocrine activation results in disordered influences of angiotensin II and catecholamines on gene expression. To assess whether angiotensin II type 1 receptor inhibition can also counteract a raised sympathetic nervous system activity, spontaneously hypertensive rats fed a hypercaloric diet were treated with eprosartan (daily 90 mg/kg body wt) and cardiovascular parameters were monitored with implanted radiotelemetry pressure transducers. Both, blood pressure and heart rate were increased (p 〈 0.05) by the hypercaloric diet. Although eprosartan reduced (p 〈 0.05) the raised systolic and diastolic blood pressure, the diet-induced rise in heart rate was blunted only partially. In addition to drugs interfering with the enhanced catecholamine influence, compounds should be considered that selectively affect cardiomyocyte gene expression via 'metabolic' signals.
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  • 23
    ISSN: 1573-4919
    Keywords: angiotensinogen ; fibronectin ; gene expression ; transcriptional regulation ; cardiomyocytes ; vascular smooth muscle cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Angiotensinogen (AGT) is a unique substrate of the renin-angiotensin system and fibronectin (FN) is an important component of the extracellular matrix. These play critical roles in the pathophysiological changes including cardiovascular remodeling and hypertrophy in response to hypertension. This study was performed to examine the regulation of AGT and FN gene in cardiac myocytes (CMs) and vascular smooth muscle cells (VSMCs) in response to mechanical stretch. Mechanical stretch significantly increased the AGT mRNA expression in CMs, while these stimuli did not affect FN mRNA levels. On the other hand, Mechanical stretch upregulated FN mRNA levels in VSMCs, whereas no increase in AGT mRNA levels was observed in response to stretch stimuli. An angiotensin II type 1 (AT1) receptor antagonist (CV11974) significantly decreased these stretch-mediated increases in mRNA level and promoter activity of the AGT and FN gene, whereas angiotensin II type 2 (AT2) receptor antagonist (PD123319) did not affect the induction. These results indicate that mechanical stretch activates transcription of the AGT and FN gene mainly via AT1 receptor-pathway in CMs and VSMCs. Furthermore, mechanisms regulating AGT and FN gene seem to be different between CMs and VSMCs.
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  • 24
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    Molecular and cellular biochemistry 212 (2000), S. 211-217 
    ISSN: 1573-4919
    Keywords: angiotensin receptor ; medullary thick ascending limb ; sodium intake ; primary cell culture ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Angiotensin II (Ang II) is an important regulator of the function of medullary thick ascending limb of loop of Henle (MTAL). Recent studies showed that changes in Ang II receptor expression occur and underlie changes in the function of proximal tubules during altered sodium intake. The present experiment was designed to determine (1) whether expression of the type 1 Ang II (AT1) receptor in the MTAL is regulated by altered sodium intake, and (2) the specific pathway(s) mediating sodium-induced AT1 expression in the MTAL. Wistar rats were fed a normal sodium (0.5%, NS), low sodium (0.07%, LS), or high sodium (4%, HS) diet for 2 weeks. Northern blot analysis and radioligand binding showed that in rats fed a normal sodium diet the rank of order for both AT1 mRNA expression and receptor density was outer medulla 〉 cortex 〉 inner medulla. Sodium restriction significantly increased both AT1 mRNA expression and receptor density in the outer medulla. In contrast, neither AT1 mRNA expression nor receptor density in the outer medulla was altered by sodium loading. Losartan treatment (3 mg/kg/per day by oral gavage for 2 weeks) prevented low sodium-induced upregulation of the AT1 receptor in the outer medulla, but it had no effect on AT1 expression in the outer medulla of rats fed a normal sodium diet. Highly purified suspensions of MTAL were isolated from rats fed a normal or low sodium diet. Low sodium intake significantly increased AT1 mRNA level by 184% and AT1 receptor density by 58% in MTALs. Primary cultures of MTAL cells were treated with PBS, Ang II (10-8 M), and Ang II + 17 octadecynoic (17 ODYA, 10 μM). Ang II caused about 2-fold increase in AT1 mRNA levels, and this increase was diminished by about 30% by the addition of 17 ODYA. We conclude that (1) sodium restriction but not sodium loading increases AT1 receptor expression in the MTAL, (2) low sodium-induced upregulation of the AT1 receptor in the MTAL is Ang II-dependent, and (3) Ang II-induced upregulation of the AT1 receptor in the MTAL is mediated, at least in part, by cytochrome P450 pathways.
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  • 25
    ISSN: 1573-4919
    Keywords: renin angiotensin system ; sarcoplasmic reticulum ; Ca2+-handling ; gene expression ; ischemia-reperfusion ; cardioprotection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of this study was to explore the possible participation of cardiac renin-angiotensin system (RAS) in the ischemia-reperfusion induced changes in heart function as well as Ca2+-handling activities and gene expression of cardiac sarcoplasmic reticulum (SR) proteins. The isolated rat hearts, treated for 10 min without and with 30 μM captopril or 100 μM losartan, were subjected to 30 min ischemia followed by reperfusion for 60 min and processed for the measurement of SR function and gene expression. Attenuated recovery of the left ventricular developed pressure (LVDP) upon reperfusion of the ischemic heart was accompanied by a marked reduction in SR Ca2+-pump ATPase, Ca2+-uptake and Ca2+-release activities. Northern blot analysis revealed that mRNA levels for SR Ca2+-handling proteins such as Ca2+-pump ATPase (SERCA2a), ryanodine receptor, calsequestrin and phospholamban were decreased in the ischemia-reperfused heart as compared with the non-ischemic control. Treatment with captopril improved the recovery of LVDP as well as SR Ca2+-pump ATPase and Ca2+-uptake activities in the postischemic hearts but had no effect on changes in Ca2+-release activity due to ischemic-reperfusion. Losartan neither affected the changes in contractile function nor modified alterations in SR Ca2+-handling activities. The ischemia-reperfusion induced decrease in mRNA levels for SR Ca2+-handling proteins were not affected by treatment with captopril or losartan. The results suggest that the improvement of cardiac function in the ischemic-reperfused heart by captopril is associated with the preservation of SR Ca2+-pump activities; however, it is unlikely that this action of captopril is mediated through the modification of cardiac RAS. Furthermore, cardiac RAS does not appear to contribute towards the ischemia-reperfusion induced changes in gene expression for SR Ca2+-handling proteins.
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  • 26
    ISSN: 1573-4919
    Keywords: pressure overload ; gene expression ; subcellular remodeling ; sarcoplasmic reticulum Ca2+-handling ; anti-hypertensive therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effects of propranolol and verapamil on contractile dysfunction, subcellular remodeling and changes in gene expression in cardiac hypertrophy due to pressure overload were examined. Rats were subjected to banding of the abdominal aorta and then treated with either propranolol (10 mg/kg daily), verapamil (5 mg/kg daily) or vehicle for 8 weeks after the surgery. Depression of the left ventricular function in the hypertrophied heart was associated with decreases in myofibrillar and myosin CA2+ ATPase activities as well as Ca2+-pump and Ca2+-release activities of the sarcoplasmic reticulum (SR). The level of a-myosin heavy chain (α-MHC) mRNA was decreased while that of β-MHC mRNA was increased in the pressure-overloaded heart. The level of SR Ca2+-pump ATPase (SERCA2) mRNA and protein content for SERCA2 were decreased in the pressure overloaded heart. Treatment of the hypertrophied animals with propranolol or verapamil resulted in preservation of the left ventricular function and prevention of the subcellular alterations. Shift in the α- and β-MHC mRNA levels and changes in the expression in SERCA2 mRNA level and protein content were also attenuated by these treatments. The results suggest that blockade of β-adrenoceptors or voltage-dependent calcium channels normalizes the cardiac gene expression, prevents subcellular remodeling and thus attenuates heart dysfunction in rats with cardiac hypertrophy. Furthermore, both cardiac β-adrenoceptors and L-type Ca2+-channels may be involved in the genesis of cardiac hypertrophy due to pressure overload.
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  • 27
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    Molecular and cellular biochemistry 213 (2000), S. 119-126 
    ISSN: 1573-4919
    Keywords: TIS11 ; an immediate early gene ; gene cloning ; gene expression ; gene organization ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The TIS11 gene is an immediate early gene that is induced rapidly and transiently by phorbol 12-myristate 13-acetate and various growth factors. To study transcriptional regulation of the gene, a genomic clone of rat TIS11 was isolated, and the organization of exon-intron structure and transcriptional initiation site were determined. The rat TIS11 gene consisted of 2 exons spanning approximately 2.5 kb. Several canonical sequences for binding of transcriptional factors were found in the 5′-flanking region. The 5.3 kb of the 5′-flanking region fused to a luciferase reporter gene showed promoter activity when introduced into rat pheochromocytoma PC12 cells. Analyses with serial 5′-deletion mutants suggested that the major positive regulatory region is located at the region of -241 to -76, and that the minimum promoter region is within the 76-bp upstream of the transcriptional initiation site. Gel mobility shift assays revealed that PC12 cell nuclear proteins specifically bind to the major positive regulatory region of the TIS11 gene. The identified nuclear protein components may act as the positive trans-acting factors in the basal expression of the TIS11 gene in PC12 cells.
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  • 28
    ISSN: 1871-4528
    Keywords: Solanum tuberosum L. ; plant development ; antioxidant genes ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The expression of antioxidant genes has been analyzed in a potato plant and during tuber dormancy. Manganese superoxide dismutase (MnSOD), cytosolic copper and zinc superoide dismutase (Cu/ZnSOD), catalase class II, cytosolic ascorbate peroxidase (APX) and glutathione peroxidase (GPX) are expressed at the RNA level in all the contexts analyzed. By contrast, the expression of the iron superoxide dismutase (FeSOD) and plastidic Cu/ZnSOD seems to be limited to green tissues, as shown by northern blots and native gels. A complex DAB-peroxidase isozyme pattern (using diaminobenzidine as substrate) has been observed in different developmental contexts analyzed, but hardly observed in tubers. During tuber dormancy, MnSOD and cytosolic Cu/ZnSOD activity was relatively constant in both Désirée and Bintje varieties while catalase activity decreases. Moreover, tuber dormancy breakage did not involve significant changes in the activity of these enzymes. On the basis of these results, the possible link between active oxygen species (AOS) metabolism and dormancy is discussed.
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  • 29
    ISSN: 1871-4528
    Keywords: gene expression ; cDNA-AFLP ; RNA-fingerprinting ; organogenesis ; tuberisation ; dormancy ; sprouting ; cluster analysis ; metabolic pathways
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Potato tuber life-cycle is composed of many individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolon formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total around 18000 transcript derived cDNA fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.
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  • 30
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    Biodiversity and conservation 9 (2000), S. 379-392 
    ISSN: 1572-9710
    Keywords: climate change ; cyanobacteria ; ectoenzymes ; Mediterranean ; nutrient limitation ; photosynthesis ; stromatolite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A stromatolitic microbial mat extensively covers La Solana streambed, a calcareous Mediterranean stream. This stromatolite shows remarkable biological and physiological diversity. It is mainly composed by cyanobacteria, with Rivularia and Schizothrix as the most abundant taxa. The stromatolite is photosynthetically adaptated to the high irradiances reaching the streambed. Photosynthetically active chlorophyll is present even in the lowest layers of the stromatolite, indicating the presence of well-preserved cyanobacteria in that part. Diffusion of gases and nutrients within the stromatolite can be possible because of the high porosity of the crust. It has been experimentally established that the stromatolite recovers heterotrophic and autotrophic activities in a few hours, after being desiccated for long periods. Recovery after desiccation is indicative of the high resilience of this community to environmental extremes, which are common in Mediterranean climatic regimes. The stromatolitic community is adapted to nutrient limitation, both to low availability of inorganic phosphorus and nitrogen (that constrain growth of primary producers), and to low dissolved organic carbon (mainly affecting heterotrophs). Stromatolitic heterotrophs mainly rely on the organic carbon stored in the crust as the main organic carbon source. These strategies are the direct response of the stromatolite to oligotrophy, and justify the restricted occurrence in stream systems affected by organic pollution.
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  • 31
    ISSN: 1573-5052
    Keywords: Calluna vulgaris ; demography ; nitrogen cycle ; climate change ; modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three Calluna vulgaris (L.) Hull heathlands (1100, 1500 and 1780 m altitude) are studied in order to establish and estimate parameters which will be integrated in a discrete event simulation of heathland functioning in the case of a 2 °C climate warming. The sites, situated in the Chaîne des Puys and the Massif du Sancy (France), present similar conditions for Calluna growth: bedrock (trachyt), exposure and slope, so that they can be compared on a climatic basis. Main parameters sampled are: age distributions, life expectancies, layering probabilities, annual growths, biomasses (standing crop), C/N rates and potential respiratory of soils. In addition, experiments were carried out on germination, hypocotyle elongation and growth of mycorrhizal fungi in order to estimate the inhibitory-to-growth action against the substitution process. On one stand (exhibiting gaps) the patch structure of Calluna plants was recorded and mapped. The rising of biomass expected is calculated according to the Aerts's model, as a function of nitrogen availability which would increase under a scenario of climate warming. From a similar point of view, layering probabilities, life expectancies and inhibition of seedlings survival modelling – under a linear model assumption – are proposed.
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  • 32
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    Water resources management 14 (2000), S. 137-156 
    ISSN: 1573-1650
    Keywords: climate adaptation ; climate change ; droughtmanagement ; England and Wales water supply
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: Abstract In this article, the relationship between U.K. watercompanies' perceptions of past climatic extremes andtheir effect on resilience to future climatic changeis explored. Perceptions and activities related topast and future dry periods was investigated throughinterviews with managers at the ten major English andWelsh water supply/sewerage companies, and severalsmaller, water-only companies. Several of thecompanies report that they have observed a trendtowards drier summers in their regions, and a number of companies say that they have observed a changetowards more intensive rainfall of shorter duration. Recent supply measures in a number of regions havebeen aimed at improving storage and distributionrelated to the perceived change in rainfall intensity.A new requirement to incorporate regional climatechange scenarios in future supply assumptions appearsto have had little impact on planning in the region todate. Many water resource planners believe that thescenarios generated are too aggregated and do notencourage a precautionary approach to planning. Somemanagers believe that records of historical droughtconditions, such as experienced in 1933/1934 or 1995/1996,as worst-case scenarios provide a better basis for planning.
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  • 33
    ISSN: 1573-5028
    Keywords: gene expression ; germination ; NADPH-cytochrome P450 reductase ; Pseudotsuga menziesii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract NADH-cytochrome P450 is a key enzyme that transfers electrons from NADPH to the cytochrome P450 family of enzymes. To begin to determine the regulation of CPR gene expression and enzyme activity in Douglas-fir a full-length cDNA was isolated from a seedling λZAP cDNA library and the ORF was used to develop a synthetic CPR-peptide-based antiserum. Northern blot analysis indicated CPR expression was regulated both developmentally prior to seed maturation and during germination, and differentially in the cotyledons, radicle and megagametophyte of seed and seedling tissues. The CPR-peptide antiserum detected a single CPR in seed and seedling microsomes analyzed by western blot of two-dimensional SDS-polyacrylamide gels. In microsomal extracts from whole seeds and seedlings, the amount of CPR protein remained constant while NADPH:cytochrome c reductase activity increased during stratification, germination and early seedling development. In contrast to cotyledons and megagametophyte, the level of CPR protein detected in radicles was higher than expected when compared to the amount of CPR transcript.
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  • 34
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    Plant and soil 224 (2000), S. 1-14 
    ISSN: 1573-5036
    Keywords: climate change ; CO2 ; decomposition ; leaf ; root ; litter ; nutrient concentration ; nutrient cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of elevated CO2 concentration ([CO2]) during plant growth on the carbon:nutrient ratios of tissues depends in part on the time and space scales considered. Most evidence relates to individual plants examined over weeks to just a few years. The C:N ratio of live tissues is found to increase, decrease or remain the same under elevated [CO2]. On average it increases by about 15% under a doubled [CO2]. A testable hypothesis is proposed to explain why it increases in some situations and decreases in others. It includes the notion that only in the intermediate range of N-availability will C:N of live tissues increase under elevated [CO2]. Five hypotheses to explain the mechanism of such increase in C:N are discussed; none of these options explains all the published results. Where elevated [CO2] did increase the C:N of green leaves, that response was not necessarily expressed as a higher C:N of senesced leaves. An hypothesis is explored to explain the observed range in the degree of propogation of a CO2 effect on live tissues through to the litter derived from them. Data on C:P ratios under elevated [CO2] are sparse and also variable. They do not yet suggest a generalising-hypothesis of responses. Although, unlike for C:N, there is no theoretical expectation that C:P of plants would increase under elevated [CO2], the average trend in the data is of such an increase. The processes determining the C:P response to elevated [CO2] seem to be largely independent of those for C:N. Research to advance the topic should be structured to examine the components of the hypotheses to explain effects on C:N. This involves experiments in which plants are grown over the full range of N and of P availability from extreme limitation to beyond saturation. Measurements need to: distinguish structural from non-structural dry matter; organic from inorganic forms of the nutrient in the tissues; involve all parts of the plant to evaluate nutrient and C allocation changes with treatments; determine resorption factors during tissue senescence; and be made with cognisance of the temporal and spatial aspects of the phenomena involved.
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  • 35
    ISSN: 1573-5036
    Keywords: ammonium ; birch ; gene expression ; nia promoter ; nitrate ; nitrate reductase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A 1535 bp promoter of the nitrate reductase gene (nia) from birch (Betula pendula) and a series of 5′ deletions were fused to the β-glucuronidase (GUS) gene and introduced into Nicotiana plumbaginifolia. In transgenic plants the NR promoter sequences directed strong GUS expression in the root epidermal hair cells, and in phloem cells of leaf and stem vascular tissue. The NR promoter confers also a significant stimulation of the GUS gene expression by nitrate. These findings might indicate that nitrate flow is one of the signals involved into tissue and cell specific expression of the NR promoter GUS fusions.
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  • 36
    ISSN: 1573-5036
    Keywords: climate change ; immobilisation ; mineralisation ; N-turnover ; nitrogen ; soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The following arguments are outlined and then illustrated by the response of the Hurley Pasture Model to [CO2] doubling in the climate of southern Britain. 1. The growth of N-limited vegetation is determined by the concentration of N in the soil mineral N pools and high turnover rates of these pools (i.e., large input and output fluxes) contribute positively to growth. 2. The size and turnover rates of the soil mineral N pools are determined overwhelmingly by N cycling into all forms of organic matter (plants, animals, soil biomass and soil organic matter — `immobilisation' in a broad sense) and back again by mineralisation. Annual system N gains (by N2 fixation and atmospheric deposition) and losses (by leaching, volatilisation, nitrification and denitrification) are small by comparison. 3. Elevated [CO2] enriches the organic matter in plants and soils with C, which leads directly to increased removal of N from the soil mineral N pools into plant biomass, soil biomass and soil organic matter (SOM). ‘Immobilisation’ in the broad sense then exceeds mineralisation. This is a transient state and as long as it exists the soil mineral N pools are depleted, N gaseous and leaching losses are reduced and the ecosystem gains N. Thus, net immobilisation gradually increases the N status of the ecosystem. 4. At the same time, elevated [CO2] increases symbiotic and non-symbiotic N2 fixation. Thus, more N is gained each year as well as less lost. Effectively, the extra C fixed in elevated [CO2] is used to capture and retain more N and so the N cycle tracks the C cycle. 5. However, the amount of extra N fixed and retained by the ecosystem each year will always be small (ca. 5–10 kg N ha-1 yr-1) compared with amount of N in the immobilisation-mineralisation cycle (ca. 1000 kg N ha-1 yr-1). Consequently, the ecosystem can take decades to centuries to gear up to a new equilibrium higher-N state. 6. The extent and timescale of the depletion of the mineral N pools in elevated [CO2] depends on the N status of the system and the magnitude of the overall system N gains and losses. Small changes in the large immobilisation—mineralisation cycle have large effects on the small mineral N pools. Consequently, it is possible to obtain a variety of growth responses within 1–10 year experiments. Ironically, ecosystem models — artificial constructs — may be the best or only way of determining what is happening in the real world.
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    Plant and soil 226 (2000), S. 219-225 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhizas ; gene expression ; Glomus mosseae ; nutrient transport processes ; plasma membrane H+-ATPases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The activity of H+-ATPases of plant and fungi generates an electrochemical gradient of H+ across the cell plasma membrane that drives a number of secondary transport systems, including those responsible for the translocation of cations, anions, amino acids and sugars. During the last years, several studies have been aimed at elucidating the role of plasma membrane H+-ATPases in the nutrient exchange processes taking place between the plant and the fungus in arbuscular mycorrhizal (AM) symbiosis. This paper reviews present knowledge about plasma membrane H+-ATPases and experimental evidence supporting the involvement of H+-ATPases of both organisms in the bidirectional transport of nutrients between partners. Molecular strategies that will provide further information on the function and regulation of plasma membrane H+-ATPases in AM symbiosis are presented and discussed.
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  • 38
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    Plant growth regulation 32 (2000), S. 27-39 
    ISSN: 1573-5087
    Keywords: ethylene ; gene expression ; jasmonic acid ; reactive oxygen species ; salicylic acid ; ultraviolet-B radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Depletion of the stratospheric ozone layer is leadingto an increase in ultraviolet-B (UV-B: 280–320 nm)radiation reaching the earth's surface. This hasraised interest in the possible consequence ofincreased UV-B levels on plant growth and developmentand the mechanisms underlying these responses. Although the effects of UV-B are now wellcharacterised at the physiological level, little isknown about the cellular and molecular mechanismsinvolved. Recent studies have shown that UV-B affectsa number of important physiological processes, such asphotosynthesis, through effects on gene expression. In addition, induction of a number of defensemechanisms, such as production of UV-B screeningpigments, increase in antioxidant enzymes andinduction of pathogenesis-related proteins, are alsomediated at the level of gene expression. The signaltransduction pathways by which UV-B regulates geneexpression are at present poorly understood. Thestudies carried out to date have, however, indicateda pivotal role for reactive oxygen species as keysecond messengers acting up-stream of a number ofpathways involving the plant hormones salicylic acid,jasmonic acid and ethylene. The transduction pathwaysidentified to date and the role of intermediates inregulating tolerance to UV-B damage are discussed inthis review.
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  • 39
    ISSN: 1573-5079
    Keywords: acclimation ; climate change ; CO2 ; down-regulation ; global change ; photosynthesis ; stomatal conductance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Atmospheric CO2 concentration continues to rise. It is important, therefore, to determine what acclimatory changes will occur within the photosynthetic apparatus of wheat (Triticum aestivum L. cv. Yecora Rojo) grown in a future high-CO2 world at ample and limited soil N contents. Wheat was grown in an open field exposed to the CO2 concentration of ambient air [370 μmol (CO2) mol−1; Control] and air enriched to ∼200 μmol (CO2) mol−1 above ambient using a Free-Air CO2 Enrichment (FACE) apparatus (main plot). A High (35 g m−2) or Low (7 and 1.5 g m−2 for 1996 and 1997, respectfully) level of N was applied to each half of the main CO2 treatment plots (split-plot). Under High-N, FACE reduced stomatal conductance (g s) by 30% at mid-morning (2 h prior to solar noon), 36% at midday (solar noon) and 27% at mid-afternoon (2.5 h after solar noon), whereas under Low-N, g s was reduced by as much as 31% at mid-morning, 44% at midday and 28% at mid-afternoon compared with Control. But, no significant CO2 × N interaction effects occurred. Across seasons and growth stages, daily accumulation of carbon (A′) was 27% greater in FACE than Control. High-N increased A′ by 18% compared with Low-N. In contrast to results for g s, however, significant CO2 × N interaction effects occurred because FACE increased A′ by 30% at High-N, but by only 23% at Low-N. FACE enhanced the seasonal accumulation of carbon (A′′) by 29% during 1996 (moderate N-stress), but by only 21% during 1997 (severe N-stress). These results support the premise that in a future high-CO2 world an acclimatory (down-regulation) response in the photosynthetic apparatus of field-grown wheat is anticipated. They also demonstrate, however, that the stimulatory effect of a rise in atmospheric CO2 on carbon gain in wheat can be maintained if nutrients such as nitrogen are in ample supply.
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  • 40
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    Plant growth regulation 31 (2000), S. 35-42 
    ISSN: 1573-5087
    Keywords: β-1,4-endoglucanase ; ethylene ; fruit ; gene expression ; polygalacturonase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fruitlet abscission during fruit development is due to the activation ofpre-differentiated abscission zones (AZs) located between twig andpedicel, and/or pedicel and pericarp. Major advances on biochemicaland molecular aspects are related to β-1,4-endoglucanase (EG) andpolygalacturonase (PG), two cell hydrolases involved in the cell walldisassemblement responsible for fruit shedding. AZ activation isaccompanied by an increase in activity and transcript accumulation ofone or both enzymes. Expression of PG genes specifically related toabscission has been found in tomato flower AZ. In peach, an EG genehighly expressed in leaf and fruitlet AZs has been isolated. AZactivation is preceded by an induction of ethylene biosynthesis,paralleled by a stimulation of ACO activity and transcript accumulation.Ethylene, besides a dramatic stimulation of PG and EG, up or downregulates several other abscission related genes. The specificexpression of genes encoding for ethylene receptors in the AZ wouldsupport the hypothesis that fruitlet AZ specificity may depend on theability of this region to sense ethylene.
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  • 41
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    Hydrobiologia 441 (2000), S. 55-62 
    ISSN: 1573-5117
    Keywords: climate change ; temperature ; mayflies ; Cloeon dipterum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Populations of the mayfly Cloeon dipterum from 48 ponds (3000 l fibre-glass tanks of 1 m depth) were monitored over the course of 1 year. To simulate possible patterns of climatic change, the ponds were subject to three temperature treatments: continuous heating to 3 °C above ambient; heating to 3 °C above ambient during the summer only; and no heating. Further experimental complexity included enhanced nutrient input into the ponds and the presence or absence of fish, giving a factorial combination of 3 temperature regimes × 2 nutrient levels × presence/absence of fish predation. Few nymphs were found in the presence of fish. Where fish were absent, the temperature treatments did not significantly affect nymph abundances, and only marginally influenced mean nymph body-lengths. In contrast, the nutrient treatment had significant effects on both nymph abundance and size, with greater numbers of generally larger nymphs occurring in those fish-free ponds receiving additional nutrients. Adult emergence began earlier in the year from the heated ponds, particularly those ponds receiving additional nutrients. Adult body-length differed between temperature treatments, but consistent patterns were difficult to ascertain because of interactions with nutrient treatment and seasonal effects. Our results show that during the short term at least, elevated temperature as a simulation of climate change does not have an overwhelming influence on either mayfly abundance or size. The influence of temperature is subtle and subject to complex interaction with other habitat variables. We therefore suggest that the direct consequences of small changes in temperature will likely be of little significance to C. dipterum, relative to indirect effects operating through interactions with predation and nutrient input.
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  • 42
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; β-glucuronidase (GUS) reporter gene ; gene expression ; isoprenoids ; mevalonate kinase ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mevalonate kinase (MVK), the enzyme that catalyzes the phosphorylation of mevalonate to produce mevalonate 5-phosphate, is considered as a potential regulatory enzyme of the isoprenoid biosynthetic pathway. The Arabidopsis thaliana MVK gene corresponding to the MVK cDNA previously isolated has been cloned and characterized. RNAse protection analysis indicated that the expression of the MVK gene generates three mRNA populations with 5′ ends mapping 203, 254 and 355 nt upstream of the MVK ATG start codon. Northern blot analysis showed that the MVK mRNA accumulates preferentially in roots and inflorescences. Histochemical analysis, with transgenic A. thaliana plants containing a translational fusion of a 1.8 kb fragment of the 5′ region of the MVK gene to the β-glucuronidase (GUS) reporter gene, indicated that the MVK 5′-flanking region directs widespread expression of the GUS gene throughout development, although the highest levels of GUS activity are detected in roots (meristematic region) and flowers (sepals, petals, anthers, style and stigmatic papillae). The expression pattern of the MVK gene suggests that the role of the encoded MVK is the production of a general pool of mevalonate-5-phosphate for the synthesis of different classes of isoprenoids involved in both basic and specialized plant cell functions. Functional promoter deletion analysis in transfected A. thaliana protoplasts indicated that regulatory elements between positions −295 and −194 of the MVK 5′-flanking region are crucial for high-level MVK gene expression.
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  • 43
    ISSN: 1573-5028
    Keywords: gene expression ; hypersensitive responses ; plant defense responses ; salicylic acid ; tobacco mosaic virus ; WRKY DNA-binding proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A pathogen- and salicylic acid (SA)-induced DNA-binding activity has been recently identified in tobacco that is related to a previously identified class of WRKY DNA-binding proteins. To identify members of the WRKY gene family associated with this DNA-binding activity, we have attempted to isolate those WRKY genes that are induced by pathogen infection. Using a domain-specific differential display procedure, we have isolated two tobacco WRKY genes, tWRKY3 and tWRKY4, that are rapidly induced in resistant tobacco plants after infection by tobacco mosaic virus (TMV). Both tWRK3 and tWRKY4 encode proteins with a single WRKY domain that contain the conserved WRKYGQK sequence. Unlike other isolated WRKY proteins that contain the Cys2His2 zinc motif, tWRKY3 and tWRKY4 appear to contain the Cys2HisCys zinc motif. Nonetheless, both tWRKY3 and tWRKY4 are capable of binding DNA molecules with the W-box (TTGAC) element recognized by other WRKY proteins. Expression of the tWRKY3 and tWRKY4 genes could be rapidly induced not only by TMV infection but also by SA or its biologically active analogues that are capable of inducing pathogenesis-related genes and enhanced resistance. Interestingly, induction of both genes by TMV infection was still observed in resistant tobacco plants expressing the bacterial salicylate hydroxylase gene (nahG), although the levels of induction appeared to be reduced. Identification of pathogen- and SA-induced genes encoding WRKY DNA-binding proteins should facilitate future studies on the regulation and functions of this novel group of DNA-binding proteins.
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  • 44
    ISSN: 1573-5028
    Keywords: gene expression ; glutamine synthetase ; legume-Rhizobium symbiosis ; nitrogen assimilation ; root nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this paper we have studied the localisation of expression of the two functional cytosolic glutamine synthetase (GS) genes, MtGSa and MtGSb, in root nodules of the model legume Medicago truncatula. We have used a combination of different techniques, including immunocytochemistry, in situ hybridisation and promoter β-glucuronidase (GUS) fusions in transgenic plants, to provide the means of correlating gene expression with protein localisation. These studies revealed that transcriptional regulation (mRNA synthesis) plays an important part in controlling GS protein levels in nodules of M. truncatula. The major locations of cytosolic GS mRNA and protein are the central tissue, the parenchyma and the pericycle of the vascular bundles. These findings indicate that in nodules, GS might be involved in other physiological processes in addition to the primary assimilation of ammonia released by the bacterial nitrogenase. The two genes show different but overlapping patterns of expression with MtGSa being the major gene expressed in the infected cells of the nodule. Promoter fragments of 2.6 kb and 3.1 kb of MtGSa and MtGSb, respectively, have been sequenced and primer extension revealed that the MtGSb promoter is expressed in nodules from an additional start site that is not used in roots. Generally these fragments in the homologous transgenic system were sufficient to drive GUS expression in almost all the tissues and cell types where GS proteins and transcripts are located except that the MtGSa promoter fragment did not express GUS highly in the nodule infected cells. These results indicate that the cis-acting regulatory elements responsible for infected-cell expression are missing from the MtGSa promoter fragment.
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  • 45
    ISSN: 1573-5028
    Keywords: epidermis ; gene expression ; glycine-rich protein ; lipid transfer protein ; proline-rich protein ; stomata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Guard cells are specialized and metabolically active cells which arise during the differentiation of the epidermis. Using Nicotiana glauca epidermal peels as a source of purified guard cells, we have constructed a cDNA library from guard cell RNA. In order to isolate genes that are predominantly expressed in guard cells, we performed a differential screen of this library, comparing the hybridization of a radiolabeled cDNA probe synthesized from guard cell RNA to that from a mesophyll cell cDNA probe. Sixteen clones were isolated based on their greater level of hybridization with the guard cell probe. Of these, eight had high homology to lipid transfer protein (LTP), two were similar to glycine-rich protein (GRP), and one displayed high homology to proline-rich proteins from Arabidopsis thaliana (AtPRP2, AtPRP4) and from potato guard cells (GPP). Northern analysis confirmed that one or more NgLTP genes, NgGRP1, and NgGPP1 are all differentially expressed, with highest levels in guard cells, and low or undetectable levels in mesophyll cells and in roots. In addition, all are induced to some degree in drought-stressed guard cells. NgLTP and NgGRP1 expression was localized by in situ hybridization to the guard cells and pavement cells in the epidermis. NgGRP1 expression was also detected in cells of the vasculature. Genomic Southern analysis indicated that LTP is encoded by a family of highly similar genes in N. glauca. This work has identified members of a subset of epidermis- and guard cell-predominant genes, whose protein products are likely to contribute to the unique properties acquired by guard cells and pavement cells during differentiation.
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  • 46
    ISSN: 1573-5028
    Keywords: chitinase function ; flower-predominant ; gene expression ; molecular cloning ; monocotyledon ; promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A flower-predominant cDNA for a gene, termed OsChia1;175, was isolated from a cDNA library of rice pistils. Northern blot and RT-PCR analyses revealed that the OsChia1;175 gene is highly expressed in floral organs (pistils, stamens and lodicules at the heading stage) but not or at an extremely low level in vegetative organs. OsChia1;175 encodes a protein that consists of 340 amino acid residues, and the putative mature protein shows 52% to 63% amino acid identity to class I chitinases of rice or other plants. The phylogenetic tree shows that the OsChia1;175 protein is a new type of plant class I chitinase in rice. The expression of OsChia1;175 in vegetative organs is not induced by several chemicals, UV, and wounding. The soluble putative mature OsChia1;175 protein expressed in Escherichia coli exhibited chitinase activity in the assay with colloidal chitin as a substrate. Genomic Southern analysis revealed that the OsChia1;175 gene was organized as a low-copy gene family. The rice genomic library was screened and a genome clone corresponding to OsChia1;175 was isolated. The transcription start sites of the OsChia1;175 gene were mapped by primer extension analysis. The 1.2 kb putative promoter region of the OsChia1;175 gene was fused to the GUS (β-glucuronidase) gene, and this chimeric gene was introduced to rice by Agrobacterium-mediated transformation. The flower-predominant gene expression was identified also in the transgenic rice plants. The high promoter activity was detected in the stigmas, styles, stamens and lodicules in transgenic plants. The possible functions of OsChia1;175 are discussed.
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  • 47
    ISSN: 1573-5028
    Keywords: anaerobiosis ; electrophoretic mobility shift assays ; gene expression ; glyceraldehyde-3-phosphate dehydrogenase ; Nicotiana tabacum ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5′ and 3′ promoter deletions were linked to a chimeric GapC4 TATA box-β-glucuronidase (GUS) reporter gene construct and transformed into tobacco. In all transgenic lines aerobic expression values were in the range obtained for negative controls while histochemical GUS assays reveal some weak expression in roots only. Anaerobic induction of about 100-fold to more than 1000-fold above unspecific background is mediated by a region of about 190 bp of the GapC4 promoter. Anaerobic reporter gene induction strongly decreases upon deletion of a 20 bp fragment from −286 to −266 relative to the transcription start point. This fragment harbours putative cis-acting sequences. Electrophoretic mobility shift assays with a 50 bp fragment harbouring these cis sequences reveal a high-mobility complex that is formed with nuclear extracts from aerobic and anaerobic leaf tissue while an additional low-mobility complex is anaerobiosis-specific. The formation of the high-mobility complex requires the sequence GTGGGCCCG. The 50 bp fragment alone confers weak and orientation-dependent anaerobic induction to a GapC4 TATA box-β-glucuronidase (GUS) reporter gene.
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  • 48
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    Plant molecular biology 43 (2000), S. 659-675 
    ISSN: 1573-5028
    Keywords: A-type cyclins ; cell cycle ; gene expression ; regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although the basic mechanisms which control the progression through the cell cycle appear to be conserved in all higher eukaryotes, the unique features of the plant developmental programme must be somehow reflected in a plant-specific regulation of the factors which control cell division. In the past few years, considerable progress has been achieved in identifying the major components of the cell cycle machinery in plants, especially the cyclin-dependent kinases (CDKs) and their regulatory subunits, the cyclins. The question of how these components direct expression of specific genes at specific stages of the cell cycle, and how they are themselves regulated, constitutes a challenge for the present and for the years to come. This review summarizes our current knowledge of a particular class of plant cyclins, the A-type cyclins, which can be further subdivided into three structural groups. The putative functions of these A-type cyclins are discussed in relation to the presence of remarkable motifs in their amino acid sequences, and to their specific transcriptional regulation, protein amount and subcellular localization.
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  • 49
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    Plant molecular biology 44 (2000), S. 73-84 
    ISSN: 1573-5028
    Keywords: auxin ; Aux/IAA ; dgt ; gene expression ; Lycopersicon esculentum ; signal transduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The diageotropica (dgt) mutation has been proposed to affect either auxin perception or responsiveness in tomato plants. It has previously been demonstrated that the expression of one member of the Aux/IAA family of auxin-regulated genes is reduced in dgt plants. Here, we report the cloning of ten new members of the tomato Aux/IAA family by PCR amplification based on conserved protein domains. All of the gene family members except one (LeIAA7) are expressed in etiolated tomato seedlings, although they demonstrate tissue specificity (e.g. increased expression in hypocotyls vs. roots) within the seedling. The wild-type auxin-response characteristics of the expression of these tomato LeIAA genes are similar to those previously described for Aux/IAA family members in Arabidopsis. In dgt seedlings, auxin stimulation of gene expression was reduced in only a subset of LeIAA genes (LeIAA5, 8, 10, and 11), with the greatest reduction associated with those genes with the strongest wild-type response to auxin. The remaining LeIAA genes tested exhibited essentially the same induction levels in response to the hormone in both dgt and wild-type hypocotyls. These results confirm that dgt plants can perceive auxin and suggest that a specific step in early auxin signal transduction is disrupted by the dgt mutation.
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  • 50
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; calcium-binding protein ; caleosin ; EF-hand ; gene expression ; lipid bodies ; vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously identified a rice gene encoding a 27 kDa protein with a single Ca2+-binding EF-hand and a putative membrane anchor. We report here similar genes termed caleosins, CLO, in other plants and fungi; they comprise a multigene family of at least five members in Arabidopsis (AtClo1–5). Northern hybridization demonstrated that AtClo2–4 mRNAs levels were low in various tissues, while AtClo1 mRNA levels were high in developing embryos and mature seeds. Analysis of transgenic Arabidopsis plants expressing the GUS reporter under control of the AtClo1 promoter showed strong levels of expression in developing embryos and also in root tip cells. Antibodies raised against AtCLO1 were used to detect caleosin in cellular fractions of Arabidopsis and rapeseed. This indicated that caleosins are a novel class of lipid body proteins, which may also be associated with an ER subdomain.
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  • 51
    ISSN: 1573-6881
    Keywords: Mitochondrial biogenesis ; copy number ; gene expression ; mitochondrial transcription factor ; nuclear—mitochondrial communication ; stimulation ; endurance training
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Mitochondrial proliferation was studied in chronically stimulated rabbit skeletal muscle over a period of 50 days. After this time, subunits of COX had increased about fourfold. Corresponding mRNAs, encoded on mitochondrial DNA as well as on nuclear genes, were unchanged when related to total tissue RNA, however, they were elevated two- to fivefold when the massive increase of ribosomes per unit mass of muscle was taken into account. The same was true for the mRNA encoding mitochondrial transcription factor A. Surprisingly, tissue levels of mtTFA protein were reduced about twofold, together with mitochondrial DNA. In conclusion, mito chondria are able to maintain high rates of mitochondrial transcription even in the presence of reduced mtTFA protein and mtDNA levels. Therefore, stimulated mtTFA gene expression accompanies stimulated mitochondrial transcription, as in other models, but it is not sufficient for an increase of mtDNA copy number and other, yet unknown, factors have to be postulated.
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  • 52
    ISSN: 1573-6857
    Keywords: dendritic polymer ; reporter gene ; gene expression ; transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclic core dendritic polymer is a new type of synthetic polymers. The ability of generation 4 of the dendrimer with a core of 1,4,7,10-tetraazacyclododecane to function as an effective gene delivery vector was investigated. Results from fluorescence in situhybridization (FISH) show that the pCH 110 plasmid DNA was transferred into human small intestine cancer metastatic ascites (HICMA) cells induced by this kind of dendrimer as a vector. The transferred LacZ, GFP and luciferase genes were highly expressed in the transfected HICMA, COS-7 and 293 cells. These studies demonstrate that the dendrimer can transfect mammalian cells in vitrowhich offers an alternatively efficient method for mammalian gene transfer.
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  • 53
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    Environmental and resource economics 15 (2000), S. 135-148 
    ISSN: 1573-1502
    Keywords: climate change ; overlapping generations models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Economics
    Notes: Abstract The artifice of an infinitely-lived representative agent iscommonly invoked to balance the present costs and future benefitsof climate stabilization policies. Since actual economies arepopulated by overlapping generations of finite-lived persons,this approach begs important questions of welfare aggregation.This paper compares the results of representative agent andoverlapping generations models that are numerically calibratedbased on standard assumptions regarding climate--economyinteractions. Under two social choice rules -- Pareto efficiencyand classical utilitarianism -- the models generate closelysimilar simulation results. In the absence of policies toredistribute income between present and future generations,efficient rates of carbon dioxide emissions abatement rise from15 to 20% between the years 2000 and 2105. Under classicalutilitarianism, in contrast, optimal control rates rise from 48 to 79% this same period.
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  • 54
    ISSN: 1573-1545
    Keywords: climate change ; mountain agriculture ; tourism ; participatory integrated assessment ; focus groups
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Winter tourism and mountain agriculture are the most important economic sectors in a major part of the Swiss Alps. Both are highly sensitive to changing climatic conditions. In the framework of the CLEAR project, results from climate impact research in the field of tourism and agricultural production were used to investigate the perception of climatic change by stakeholders and to assess possible adaptations. We used a participatory integrated assessment (PIA) to involve the knowledge, values and experiences of the various social actors in tourism and agriculture (e.g., skiers, tourism managers, farmers) in the research process. Whereas climate change may have various severe direct impacts on the tourism industry, depending on the region, agricultural production may generally benefit from changed climatic conditions. But because of the dependence of farmers on “off-farm” income, the loss due to declining winter tourism in specific areas may cause more important indirect effects. However, the two sectors may adapt actively by choosing from a variety of strategies, and the loss of income from the tourism industry may support the re-evaluation of the various functions agriculture plays in mountain regions, beyond the production of food. The study demonstrates the suitability of the PIA approach to elucidate the interactions between different stakeholders and their perception of the climate change phenomena. A similar participatory approach could be a useful tool to transfer research results and expert knowledge to the political process addressing adaptations to climate change.
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  • 55
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    Environmental and resource economics 17 (2000), S. 163-181 
    ISSN: 1573-1502
    Keywords: carbon emissions inequality ; climate change ; global warming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Economics
    Notes: Abstract This paper analyzes future carbon emissions inequality using a group decomposition of the Gini index. Business-as-usual projections to the year 2100 for 135 countries show inequality in per capita emissions declines slowly. Next, the impact on emissions levels and inequality of the Kyoto Protocol and other abatement proposals for Annex II countries in 2010 are measured, with a focus on the gap-narrowing and reranking effects. Substantial reranking of per capita emissions between Annex II and non-Annex II countries will not occur unless the former reduce their emissions by at least 50% (versus 1990 levels) and the latter continue growing unabated.
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    Integrated assessment 1 (2000), S. 127-136 
    ISSN: 1573-1545
    Keywords: integrated assessments ; climate change ; discounting ; equity ; climate policy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A standard framework is presented as an underlying model for the discounting debate. Views and proposals for the techniques and rates of discounting are assessed. Alternative modeling frameworks for studying intergenerational equity issues are evaluated with the result that the basic insights they provide do not differ very much. Results from model experiments involving different discount rate proposals show that fudging the discount rate does not lead to efficient climate policy. Three major clusters of opinions are identified regarding the applicability of cost-benefit analysis to the climate change problem and the appropriate discount rate to use. It is concluded that under some very special circumstances the cost-benefit rule should be abandoned and cost-effective strategies implying standard discount rates should be sought to reach clearly defined and justified environmental targets.
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  • 57
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    Mitigation and adaptation strategies for global change 5 (2000), S. 361-377 
    ISSN: 1573-1596
    Keywords: carbon budget ; climate change ; desertification ; international environmental institutions ; land degradation ; research convergence ; science policy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Poor knowledge of links between desertification and globalclimate change is limiting funding from the Global Environment Facility foranti-desertification projects and realization of synergies between theConvention to Combat Desertification (CCD) and the FrameworkConvention on Climate Change (FCCC). Greater convergence betweenresearch in the two fields could overcome these limitations, improve ourknowledge of desertification, and benefit four areas of global climate changestudies: mitigation assessment; accounting for land cover change in thecarbon budget; land surface-atmosphere interactions; and climate changeimpact forecasting. Convergence would be assisted if desertification weretreated more as a special case in dry areas of the global process of landdegradation, and stimulated by: (a) closer cooperation between the FCCCand CCD; (b) better informal networking between desertification and globalclimate change scientists, e.g. within the framework of theIntergovernmental Panel on Climate Change (IPCC). Both strategies wouldbe facilitated if the FCCC and CCD requested the IPCC to provide ascientific framework for realizing the synergies between them.
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  • 58
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    Mitigation and adaptation strategies for global change 5 (2000), S. 379-406 
    ISSN: 1573-1596
    Keywords: climate change ; Ethiopia ; historical analogy ; migration ; vulnerability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Abstract Climate change has been presented as a likely trigger formigration of people, especially in dryland areas of less developed countries.The underlying research questions focus on the strength of adaptationcapacity of subsistence farmers in Northern Ethiopia, and evaluate historicalexperiences gained from drought-induced migration. Through a survey of104 peasants who had to migrant due to persistent drought, vulnerabilityto climate change has shown to be a complex issue, including themultiplicity of factors comprising a household environment. Still, to bevulnerable does not make someone a potential climate migrant, as peoplein marginal regions have developed a great variety of adaptationmechanisms, which strengthen their ability to cope with both, slow climaticchanges and extreme climatic events.
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  • 59
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    Nutrient cycling in agroecosystems 57 (2000), S. 107-117 
    ISSN: 1573-0867
    Keywords: N-fertilizer ; nitrous oxide ; climate change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In most soils, formation and emissions of N2O to the atmosphere are enhanced by an increase in available mineral nitrogen (N) through increased rates of nitrification and denitrification. Therefore, addition of N, whether in the form of organic or inorganic compounds eventually leads to enhanced N2O emissions. Global N2O emissions from agricultural systems have previously been related primarily to fertilizer N input from synthetic sources. Little attention has been paid to N input from other N sources or to the N2O produced from N that has moved through agricultural systems. In a new methodology used to estimate N2O emissions on the country or regional scale, that is briefly described in this paper, the anthropogenic N input data used include synthetic fertilizer, animal waste (feces and urine) used as fertilizer, N derived from enhanced biological N-fixation through N2 fixing crops and crop residue returned to the field. Using FAO database information which includes data on synthetic fertilizer consumption, live animal production and crop production and estimates of N input from recycling of animal and crop N, estimates of total N into Asian agricultural systems and resulting N2O emissions are described over the time period 1961 through 1994. During this time the quantity and relative amounts of different types of materials applied to agricultural soils in Asia as nitrogen (N) fertilizer have changed dramatically. In 1961, using the earliest entry from the FAO database, of the approximately 15.7 Tg of fertilizer N applied to agricultural fields 2.1 Tg N (13.5% of total N applied) was from synthetic sources, approximately 6.9 Tg N from animal wastes, 1.7 Tg N from biological N-fixation, and another 5 Tg N from reutilization of crop residue. In 1994, 40.2 Tg from synthetic fertilizer N (57.8% of total), 14.2 Tg from animal wastes, 2.5 Tg from biological N-fixation and 12.6 Tg from crop residue totalling 69.5 Tg N were utilized within agricultural soils in all Asian countries. The increases in N utilization have increased the emission of nitrous oxide from agricultural systems. Estimated N2O from agricultural systems in Asia increased from about 0.8 Tg N2O-N in 1961 to about 2.1 in 1994. The period of time when increases in N input and resulting N2O emissions were greatest was during 1970–1990. This evaluation of N input into Asian agricultural systems and the resulting N2O emissions demonstrates the large change in global agriculture that has occurred in recent decades. Because of the increased need for food production increases in N input are likely. Although the rate of increase of N input and N2O emissions during the 1990s appears to have declined, we ask if this slowed rate of increase is a general long term trend or if global food production pressures will tend to accelerate N input demand and resulting N2O emissions as we move into the 21st century.
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  • 60
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    Water resources management 14 (2000), S. 177-189 
    ISSN: 1573-1650
    Keywords: climate change ; Sweden ; water balance models ; water resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: Abstract This article describes investigationsinto the effects of climate change on flow regimes oftwenty-five catchments (from 6 to 1293 km2) incentral Sweden. Hydrological responses of fifteenhypothetical climate change scenarios (e.g.combinations of ΔT = +1, +2 and +4 °C andΔP = 0, ± 10%, ± 20%) were simulated by a conceptual monthly water balance model. The results suggest thatall the hypothetical climate change scenarios wouldcause major decreases in winter snow accumulation.Significant increase of winter flow and decrease ofspring and summer runoff were resulted from mostscenarios. Attendant changes in actualevapotranspiration were also examined for all climatechange scenarios. Despite the changes in seasonaldistribution of evapotranspiration, the change inannual total evapotranspiration was relatively smallwith the maximum change of 23% compared with the 76%for mean annual snow water equivalent changes and 52%for mean annual runoff changes. Such hydrologicresults would have significant implications on futurewater resources design and management.
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  • 61
    ISSN: 1573-0662
    Keywords: aerosols ; cloud formation ; microanalysis ; ship tracks ; climate change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The chemical composition of individual particles 〉0.2 μm sampled duringthe MAST-experiment wereanalysed by SEM-EDX, in combination with multivariatetechniques. The objective of this experiment was toidentify the mechanisms responsible for themodification of marine stratocumulus clouds byemissions from ships and in a wider sense to provideinformation on the global processes involved inatmospheric modification of cloud albedo. Aerosolswere examined under different MBL pollution levels(clean, intermediately polluted and moderatelypolluted) in five different reservoirs: backgroundbelow-cloud and above-cloud aerosol; background clouddroplet residual particles; below-cloud ship plumeaerosol and ship track cloud droplet residualparticles.In this study a relation was provided between theaerosol emitted from the ship's stack to an effect incloud. Additionally, a large fraction of the ambientaerosol was found to be composed of organic materialor other compounds, consisting of low Z-elements,associated with chlorine. Their number fraction waslargest in clean marine boundary layers, and decreasedwith increasing pollution levels. The fraction of`transformed sea salt' (Na, Cl, S), on the other hand,increased with the pollution level in the MBL. Only20% of the particles fell within the detectable rangeof the analysis.
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  • 62
    ISSN: 1572-9699
    Keywords: differentiation ; FtsZ ; gene expression ; septum ; SsgA ; transmission electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes the effects of increased expression of the cell division genes ftsZ, ftsQ, and ssgA on the development of both solid- and liquid-grown mycelium of Streptomyces coelicolor and Streptomyces lividans. Over-expression of ftsZ in S. coelicolor M145 inhibited aerial mycelium formation and blocked sporulation. Such deficient sporulation was also observed for the ftsZ mutant. Over-expression of ftsZ also inhibited morphological differentiation in S. lividans 1326, although aerial mycelium formation was less reduced. Furthermore, antibiotic production was increased in both strains, and in particular the otherwise dormant actinorhodin biosynthesis cluster of S. lividans was activated in liquid- and solid-grown cultures. No significant alterations were observed when the gene dosage of ftsQ was increased. Analysis by transmission electron microscopy of an S. coelicolor strain over-expressing ssgA showed that septum formation had strongly increased in comparison to wild-type S. coelicolor, showing that SsgA clearly influences Streptomyces cell division. The morphology of the hyphae was affected such that irregular septa were produced with a significantly wider diameter, thereby forming spore-like compartments. This suggests that ssgA can induce a process similar to submerged sporulation in Streptomyces strains that otherwise fail to do so. A working model is proposed for the regulation of septum formation and of submerged sporulation.
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  • 63
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    Plant growth regulation 30 (2000), S. 193-200 
    ISSN: 1573-5087
    Keywords: ACC synthase ; ACC oxidase ; ethylene ; fruit ; gene expression ; regulation ; ripening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Progress in ethylene regulating fruit ripening concerning itsperception and signal transduction and expression of ACC synthaseand ACC oxidase genes is reviewed. ACC synthase and ACC oxidasehave been characterized and their genes cloned from various fruittissues. Both ACC synthase and ACC oxidase are encoded bymultigene families, and their activities are associated withfruit ripening. In climacteric fruit, the transition toautocatalytic ethylene production appears to be due to a seriesof events in which ACC sythase and ACC oxidase genes have beenexpressed developmentally. Differential expression of ACCsynthase and ACC oxidase gene family members is probably involvedin such a transition that ultimately controls the onset of fruitripening.In comparison to ACC synthase and ACC oxidase, less is knownabout ethylene perception and signal transduction because of thedifficulties in isolating and purifying ethylene receptors orethylene-binding proteins using biochemical methods. However, theidentification of the Nr tomato ripening mutant as anethylene receptor, the applications of new potent anti-ethylenecompounds and the generation of transgenic fruits with reducedethylene production have provided evidence that ethylenereceptors regulate a defined set of genes which are expressedduring fruit ripening. The properties and functions of ethylenereceptors, such as ETR1, are being elucidated.Application of molecular genetics, in combination withbiochemical approaches, will enable us to better understand theindividual steps leading from ethylene perception and signaltransduction and expression of ACC synthase and ACC oxidase genefamily member to the physiological responses.
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  • 64
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    Genetic resources and crop evolution 47 (2000), S. 135-140 
    ISSN: 1573-5109
    Keywords: Aegilops tauschii ; gene expression ; genetic inheritance ; Puccinia recondita f. sp. tritici ; rust resistance ; synthetic hexaploid wheats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A collection of 164 Aegilops tauschii accessions, obtained from Gatersleben, Germany, was screened for reaction to leaf rust under controlled greenhouse conditions. We have also evaluated a selection of synthetic hexaploid wheats, produced by hybridizing Ae. tauschii with tetraploid durum wheats, as well as the first and second generation of hybrids between some of these resistant synthetic hexaploid wheats and susceptible Triticum aestivum cultivars. Eighteen (11%) accessions of Ae. tauschii were resistant to leaf rust among which 1 was immune, 13 were highly resistant and 4 were moderately resistant. Six of the synthetic hexaploid wheats expressed a high level of leaf rust resistance while four exhibited either a reduced or complete susceptibility compared to their corresponding diploid parent. This suppression of resistance at the hexaploid level suggests the presence of suppressor genes in the A and/or B genomes of the T. turgidum parent. Inheritance of leaf rust resistance from the intercrosses with susceptible bread wheats revealed that resistance was dominant over susceptibility. Leaf rust resistance from the three synthetics (syn 101, syn 701 and syn 901) was effectively transmitted as a single dominant gene and one synthetic (syn 301) possessed two different dominant genes for resistance.
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  • 65
    ISSN: 1573-5044
    Keywords: fall dormancy ; gene expression ; Medicago sativa L. ; protein ; starch ; sugar ; winter hardiness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A major factor limiting persistence of alfalfa (Medicago sativa L.) in the northern US is poor winter hardiness. Our hypothesis is that suspension cell cultures derived from dormant, winter-hardy alfalfa cultivars would cold acclimate and survive sub-zero temperatures better than cell cultures derived from non-dormant, non-hardy cultivars. Our objectives were (1) to determine if genetic differences in winter hardiness between dormant and non-dormant alfalfa were retained by suspension cells derived from these contrasting cultivars; and (2) to determine the physiological and biochemical bases for differences in freezing tolerance of suspension cells. Cell suspensions derived from `5262' (fall dormant) and `5929' (fall non-dormant) were cold hardened at 2 °C for 14 days. Cells were frozen in a cooling bath and cell survival determined by measuring 2, 3, 5-triphenyltetrazolium chloride (TTC) reduction. Cold acclimation improved cell survival of both cultivars to −5 °C when compared to unacclimated cells. Only acclimated cells of 5262 survived temperatures of −10 °C to −25 °C. The freezing tolerance of cold-acclimated 5262 cells was associated with high sugar and starch concentrations, lower α-amylase activities and slightly lower cell protein levels when compared to 5929. No differences in polypeptide composition were evident when comparing acclimated and unacclimated cells of 5929, but polypeptide composition did change with acclimation of 5262 cells. As expected, expression of RootCAR1 in 5262 cells increased with cold acclimation, but high levels of RootCAR1 transcript were unexpectantly found in both cold acclimated and unacclimated 5929 cells. With the exception of the RootCAR1 expression, many of the physiological responses of these alfalfa cell lines to cold acclimation were similar to those that have been reported for field-grown plants.
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  • 66
    ISSN: 1573-5028
    Keywords: C4 photosynthesis ; maize ; phosphoenolpyruvate carboxylase ; transgenic plant ; transcription ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract C4-type phosphenolpyruvate carboxylase (C4PEPC) acts as a primary carbon assimilatory enzyme in the C4 photosynthetic pathway. The maize C4PEPC gene (C4Ppc1) is specifically expressed in mesophyll cells (MC) of light-grown leaves, but the molecular mechanism responsible for its cell type-specific expression has not been characterized. In this study, we introduced a chimeric maize C4Ppc1 5′-flanking region/β-glucuronidase (GUS) gene into maize plants by Agrobacterium-mediated transformation. Activity assay and histochemical staining showed that GUS is almost exclusively localized in leaf MC of transgenic maize plants. This observation suggests that the introduced 5′ region of maize C4Ppc1 contains the necessary cis element(s) for its specific expression in MC. Next, we investigated whether the 5′ region of the maize gene interacts with nuclear proteins in a cell type-specific manner. By gel shift assays with nuclear extracts prepared from MC or bundle sheath cells (BSC), cell type-specific DNA-protein interactions were detected: nuclear factors PEPIb and PEPIc are specific to MC whereas PEPIa and PEPIIa are specific to BSC. Light alters the binding activity of these factors. These interactions were not detected in the assay with nuclear extract prepared from root, or competed out by oligonucleotides corresponding to the binding sites for the maize nuclear protein, PEP-I, which is known to bind specifically to the promoter region of C4Ppc1. The results suggest that novel cell type-specific positive and negative nuclear factors bind to the maize C4Ppc1 5′-flanking region and regulate its differential transcription in MC in a light-dependent manner.
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  • 67
    ISSN: 1573-5028
    Keywords: gene expression ; nicotinic acid ; pyridine alkaloids ; secondary metabolism ; polyploidy ; wound-induced
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quinolate acid phosphoribosyltransferase (QPRTase), a key enzyme in nicotinamide adenine dinucleotide (NAD) biosynthesis, also plays an important role in ensuring nicotinic acid is available for the synthesis of defensive pyridine alkaloids in Nicotiana species. In this study, cDNAs for QPRTase were characterized from N. rustica and N. tabacum. Deduced proteins from both cDNAs are almost identical and contain a 24 amino acid N-terminal extension, not reported in other QPRTases, that has characteristics of a mitochondrial targeting sequence. In N. tabacum and N. sylvestris, both of which contain nicotine as the major pyridine alkaloid, QPRTase transcript was detected in roots, the site of nicotine synthesis, but not in leaves. QPRTase transcript levels increased markedly in roots of both species 12–24 h after damage to aerial tissues, with a concomitant rise in transcript levels of putrescine N-methyltransferase (PMT), another key enzyme in nicotine biosynthesis. In N. glauca, however, in which anabasine represents the major pyridine alkaloid, QPRTase transcript was detected in both leaf and root tissues. Moreover, wound induction of QPRTase but not PMT was observed in leaf tissues, and not in roots, 12–24 h after wounding. Southern analysis of genomic DNA from the Nicotiana species noted above, and also several others from within the genus, suggested that QPRTase is encoded by a small gene family in all the species investigated.
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  • 68
    ISSN: 1573-5036
    Keywords: CO2 enrichment ; carbon storage ; climate change ; dissolved organic carbon ; nitrogen fixation ; root exudate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root exudation has been hypothesized as one possible mechanism that may lead to increased inputs of organic C into the soil under elevated atmospheric CO2, which could lead to greater long-term soil C storage. In this study, we analyzed exudation of dissolved organic C from the roots of seedlings of the N-fixing tree Robinia pseudoacacia L. in a full factorial design with 2 CO2 (35.0 and 70.0 Pa) × 2 temperature (26° and 30 °C during the day) × 2 N fertilizer (0 and 10.0 mM N concentration) levels. We also analyzed the decomposition rates of root exudate to estimate gross rates of exudation. Elevated CO2 did not affect root exudation of organic C. A 4 °C increase in temperature and N fertilization did, however, significantly increase organic C exudation rates. Approximately 60% of the exudate decomposed relatively rapidly, with a turnover rate of less than one day, while the remaining 40% decomposed more slowly. These results suggest that warmer climates, as predicted for the next century, may accelerate root exudation of organic C, which will probably stimulate rapid C cycling and may make a minor contribution to intermediate to more long-term soil C storage. However, as these losses to root exudation did not exceed 1.2% of the net C fixed by Robinia pseudoacacia, root exudation of organic C appears to have little potential to contribute to long-term soil C sequestration.
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  • 69
    ISSN: 1573-5036
    Keywords: atmospheric carbon dioxide ; climate change ; litter quality ; resorption ; retranslocation ; senescence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The prediction that litter quality, and hence litter decomposition rates, would be reduced when plants are grown in a CO2-enriched atmosphere has been based on the observation that foliar N concentrations usually are lower in elevated [CO2]. The implicit assumption is that the N concentration in leaf litter reflects the N concentration in green leaves. Here we evaluate that assumption by exploring whether the process of seasonal nutrient resorption is different in CO2-enriched plants. Nitrogen resorption was studied in two species of maple trees (Acer rubrum L. and A. saccharum Marsh.), which were planted in unfertilized soil and grown in open-top chambers with ambient or elevated [CO2] in combination with ambient or elevated temperature. In the second growing season, prior to autumn senescence, individual leaves were collected and analyzed for N and dry matter content. Other leaves at the same and an adjacent node were collected for analysis as they senesced and abscised. This data set was augmented with litter samples from the first growing season and with green leaves and leaf litter collected from white oak (Quercus alba L.) saplings grown in ambient and elevated [CO2] in open-top chambers. In chambers maintained at ambient temperature, CO2 enrichment reduced green leaf N concentrations by 25% in A. rubrum and 19% in A. saccharum. CO2 enrichment did not significantly reduce resorption efficiency so the N concentration also was reduced in litter. There were, however, few effects of [CO2] on N dynamics in these leaves; differences in N concentration usually were the result of increased dry matter content of leaves. The effects of elevated [CO2] on litter N are inherently more difficult to detect than differences in green leaves because factors that affect senescence and resorption increase variability. This is especially so when other environmental factors cause a disruption in the normal progress of resorption, such as in the first year when warming delayed senescence until leaves were killed by an early frost. The results of this experiment support the approach used in ecosystem models in which resorption efficiency is constant in ambient and elevated [CO2], but the results also indicate that other factors can alter resorption efficiency.
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  • 70
    ISSN: 1573-5028
    Keywords: gene expression ; heterologous expression ; H+/hexose symporter ; Lycopersicon esculentum ; quantitative PCR ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length (LeHT2) and two partial (LeHT1 and LeHT3) cDNA clones, encoding hexose transporters, were isolated from tomato (Lycopersicon esculentum) fruit and flower cDNA libraries. Southern blot analysis confirmed the presence of a gene family of hexose transporters in tomato consisting of at least three members. The full-length cDNA (LeHT2) encodes a protein of 523 amino acids, with a calculated molecular mass of 57.6 kDa. The predicted protein has 12 putative membrane-spanning domains and belongs to the Major Facilitator Superfamily of membrane carriers. The three clones encode polypeptides that are homologous to other plant monosaccharide transporters and contain conserved amino acid motifs characteristic of this superfamily. Expression of the three genes in different organs of tomato was investigated by quantitative PCR. LeHT1 and LeHT3 are expressed predominantly in sink tissues, with both genes showing highest expression in young fruit and root tips. LeHT2 is expressed at relatively high levels in source leaves and certain sink tissues such as flowers. LeHT2 was functionally expressed in a hexose transport-deficient mutant (RE700A) of Saccharomyces cerevisiae. LeHT2-dependent transport of glucose in RE700A exhibited properties consistent with the operation of an energy-coupled transporter and probably a H+/hexose symporter. The K m of the symporter for glucose is 45 μM.
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  • 71
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; β-glucuronidase (GUS) reporter gene ; farnesyl diphosphate synthase ; gene expression ; isoprenoids ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Farnesyl diphosphate synthase (FPS), the enzyme that catalyses the synthesis of farnesyl diphosphate (FPP) from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), is considered a regulatory enzyme of plant isoprenoid biosynthesis. The promoter regions of the FPS1 and FPS2 genes controlling the expression of isoforms FPS1S and FPS2, respectively, were fused to the β-glucuronidase (GUS) reporter gene and introduced into Arabidopsis thaliana plants. The FPS1S:GUS gene is widely expressed in all plant tissues throughout development, thus supporting a role for FPS1S in the synthesis of isoprenoids serving basic plant cell functions. In contrast, the FPS2:GUS gene shows a pattern of expression restricted to specific organs at particular stages of development. The highest levels of GUS activity are detected in flowers, especially in pollen grains, from the early stages of flower development. After pollination, much lower levels of GUS activity are detected in the rest of floral organs, with the exception of the ovary valves, which remain unstained throughout flower development. GUS activity is also detected in developing and mature seeds. In roots, GUS expression is primarily detected at sites of lateral root initiation and in junctions between primary and secondary roots. No GUS activity is detected in root apical meristems. GUS expression is also observed in junctions between primary and secondary stems. Overall, the pattern of expression of FPS2:GUS suggests a role for FPS2 in the synthesis of particular isoprenoids with specialized functions. Functional FPS2 gene promoter deletion analysis in transfected protoplasts and transgenic A. thaliana plants indicate that all the cis-acting elements required to establish the full pattern of expression of the FPS2 gene are contained in a short region extending from positions −111 to +65. The potential regulatory role of specific sequences within this region is discussed.
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    Journal of aquatic ecosystem stress and recovery 7 (2000), S. 91-109 
    ISSN: 1573-5141
    Keywords: climate change ; industrial fisheries ; marine monitors ; pollution ; Seabirds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a range of anthropogenic stressors thatimpact seabirds, review the effects of these stressorson individuals and populations and discuss the roleand value of seabirds as monitors of marine ecosystemhealth. Stressors described are restricted to thosewhich affect seabirds directly or indirectly throughthe marine environment; we have not dealt withterrestrially based stressors such as introducedmammalian predators or loss of habitat, which canpotentially affect seabirds whilst breeding. Wediscuss three broad categories of stress in seabirds.Marine pollutants (including biologicallynon-essential heavy metals, oil, organic pesticidesand polychlorinated biphenyls (PCBs), and plastics),industrial fisheries (further divided into the effectsof depletion of prey stocks and direct mortality), andclimate change. Additionally we highlight the role ofseabirds as monitors of marine ecosystem health,taking the example of long-term mercury contaminationas a case study. We conclude that seabirds are exposedto an increasing array of potential stressors, andthat the impact of a particular source of stress onseabirds varies markedly between species in relationto foraging and breeding ecology. The most seriousthreat to seabirds is direct mortality of adultsresulting from industrial and commercial fishingactivities. In some cases this is a significant threatto individual populations or even entire species.
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  • 73
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    Environmental modeling and assessment 5 (2000), S. 157-168 
    ISSN: 1573-2967
    Keywords: climate change ; crops ; carbon dioxide ; economic welfare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Increasing concentrations of atmospheric carbon dioxide CO2 have a beneficial effect on crop production that would tend to offset some of the economic losses that might be generated in some areas by the climatic effects of atmospheric CO2 and other greenhouse gases. Previous estimates of the economic benefits of CO2 fertilization on world crop production, however, were based on the assumption that percent changes in supply are equal to percent changes in yield. This assumption is not valid, however, because it confounds changes in supply with changes in quantity supplied. This error leads to an overestimation of the real economic benefits of CO2 fertilization by 61–166%. The effects of CO2 fertilization on crop production, therefore, will reduce some of the potential damages caused by the climatic impacts of greenhouse gases, but by significantly less than that indicated in earlier research.
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    Environmental monitoring and assessment 61 (2000), S. 133-144 
    ISSN: 1573-2959
    Keywords: adaptation scenarios ; climate change ; agriculture ; Poland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This paper demonstrates the ability of Polish agriculture to adapt to predicted climate change according to GISS and GFDL scenarios. Both climate-change scenarios will significantly affect farming conditions in Poland through water deficit, shifts in planting and harvesting seasons, changes in crop yields and crop structure. Neither scenario seems to endanger the self-sufficiency of Poland as long as preventative measures are taken. Moreover, the realization of GISS creates the possibility of a surplus in production. It must be emphasized that regardless of the scenario, the adaptation of agriculture to an expected climate change cannot be handled by the farming community itself.
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    Environmental monitoring and assessment 61 (2000), S. 161-166 
    ISSN: 1573-2959
    Keywords: adaptation measures ; water resources ; climate change ; Central Asia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A large part of the Central Asian region is located within the inner flow of the Aral Sea basin. The water resources are formed from renewed superficial and underground waters of natural origin, and also with returnable waters. The intensive increase of water intake, that took place in the second half of the twentieth century caused practically complete assimilation of the river inflow. That was the main reason for the Aral Sea crisis. On the basis of the analysis of long periodical rows of observation by meterological and hydrological stations, the estimation of regional water resources and calculations of changes of some components of the hydrological cycle due to the expected climate changes are presented. Measures for adaptation in the southern part of the Aral Sea region are considered.
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    Environmental monitoring and assessment 61 (2000), S. 187-191 
    ISSN: 1573-2959
    Keywords: climate change ; adaptation ; water resources ; China
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract It is indicated that up to the year 2030, the annual average temperatures in China will increase by 0.88 to 1.2°C, with increments in the south less than in the north. Annual average precipitation would raise slightly, but the increment could be 4% in northeastern China. The increment of annual mean runoff could rise over 6% in the northeastern area, and decrease in the other regions 1.4 to 10.5%. The increased water shortage due to climate change could achieve 160 to 5090 million m3 in some areas of China. Financial loss due to the lack of water could reach 1300 million yuan, and up to 4400 million yuan in serious drought years in the Beijing-Tianjin-Tangshan area.
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    Environmental monitoring and assessment 62 (2000), S. 261-272 
    ISSN: 1573-2959
    Keywords: boreal forests ; climate change ; ecosystem theory ; nonlinear thermodynamics ; resource management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Over the past two centuries progressive anthropogenicperturbations to natural, boreal forest-climateinteractions have contributed increasingly to awidespread transition from coniferous to deciduouslandscapes. This transition is associated withchanges, at boreal latitudes, in temperature,photosynthetic activity, atmospheric CO2 andspring snow cover. These signals convey thediminished capacity of boreal landscapes to store andcycle materials and energy.
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    Environmental monitoring and assessment 61 (2000), S. 9-35 
    ISSN: 1573-2959
    Keywords: climate change ; risk ; adaptation ; thresholds ; limits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Adaptation in response to anthropogenic climate change seeks to maintain viability by maximising benefits and minimising losses. It is necessary because some climatic change is now inevitable, despite the international focus on mitigation measures. Indeed, the measures agreed at Kyoto would by themselves result in only a small reduction in the climate changes to be expected over the next century. Discussion of the expected changes and possible impacts leads to the following conclusions regarding climate change scenarios in relation to impacts and adaptation: • Climate change in the foreseeable future will not be some new stable "equilibrium" climate, but rather an ongoing "transient" process; • Climate change predictions relevant to impacts on most sectors and ecosystems are still highly uncertain; • There is a need for a greater focus on developing countries and tropical regions, and on relevant key variables, including the magnitude and frequency of extreme events; • The focus should shift from single predictions, or extreme ranges of uncertainty, to risk assessment; • Thresholds critical to impacted sectors and ecosystems should be identified, and expressed as functions of climatic variables; • Planned adaptations will be necessary to cope with multiple stresses, including those due to non-climatic changes; • A major task of adaptation science is to identify the limits of adaptation, i.e., to identify "dangerous levels of greenhouse gases" beyond which adaptation becomes impractical or prohibitively expensive.
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    Environmental monitoring and assessment 61 (2000), S. 75-92 
    ISSN: 1573-2959
    Keywords: climate change ; mitigation ; market instruments ; Costa Rica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Two decades of developing relevant legal and institutional regimes for the sustainable and nondestructive use of natural resources have framed Costa Rica's pioneer approach to mitigate climate change and conserve its rich biological diversity. This policy framework provides an appropriate context for the actual and proposed development of market instruments designed to attract capital investments for carbon sequestration and biodiversity conservation, and allows the establishment of mechanisms to use those funds to compensate owners for the environmental services provided by their land. As a developing economy. Costa Rica is striving to internalize the benefits from the environmental services it offers, as a cornerstone of its sustainable development strategy.
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    Environmental monitoring and assessment 61 (2000), S. 65-74 
    ISSN: 1573-2959
    Keywords: biodiversity ; climate variability ; climate change ; adaptation ; institutional reform
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract If current trends continue, human activities will drastically alter most of the planet's remaining natural ecosystems and their composite biota within a few decades. Compounding the impacts on biodiversity from deleterious management practices is climate variability and change. The Intergovernmental Panel on Climate Change (IPCC) recently concluded that there is ample evidence to suggest climate change is likely to result in significant impacts on biological diversity. These impacts are likely to be exacerbated by the secondary effects of climate change such as changes in the occurrence of wildfire, insect outbreaks and similar disturbances. Current changes in climate are very different from those of the past due to their rate and magnitude, the direct effects of increased atmospheric CO2 concentrations and because highly modified landscapes and an array of threatening processes limit the ability of terrestrial ecosystems and species to respond to changed conditions. One of the primary human adaptation option for conserving biodiversity is considered to be changes in management. The complex and overarching nature of climate change issues emphasises the need for greatly enhanced cooperation between scientists, policy makers, industry and the community to better understand key interactions and identify options for adaptation. A key challenge is to identify opportunities that facilitate sustainable development by making use of existing technologies and developing policies that enhance the resilience of climate-sensitive sectors. Measures to enhance the resilience of biodiversity must be considered in all of these activities if many ecosystem services essential to humanity are to be sustained. New institutional arrangements appear necessary at the regional and national level to ensure that policy initiatives and research directed at assessing and mitigating the vulnerability of biodiversity to climate change are complementary and undertaken strategically and cost-effectively. Policy implementation at the national level to meet responsibilities arising from the UNFCCC (e.g., the Kyoto Protocol) and the UN Convention on Biological Diversity require greater coordination and integration between economic sectors, since many primary drivers of biodiversity loss and vulnerability are influenced at this level. A case study from the Australian continent is used to illustrate several key issues and discuss a basis for reform, including recommendations for facilitating adaptation to climate variability and change.
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    Environmental monitoring and assessment 61 (2000), S. 49-64 
    ISSN: 1573-2959
    Keywords: climate change ; El Niño Southern Oscillation ; public health ; adaptation ; primary prevention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Global climate change is likely to have a range of consequences for human health as a result of disturbance or weakening of the biosphere's natural or human-managed life support systems. The full range of potential human health impacts of global climate change is diverse and would be distributed differentially spatially and over time. Changes in the mortality toll of heatwaves and changes in the distribution of vector-borne infectious diseases may occur early. The public health consequences of sea level rise and of regional changes in agricultural productivity may not occur (or become apparent) for several decades. Vulnerability is a measure of both sensitivity to climate change and the ability to adapt in anticipation of, or in response to, its impacts. The basic modes of adaptation to climate-induced health hazards are biological, behavioural and social. Adaptation can be undertaken at the individual, community and whole-population levels. Adaptive strategies should not introduce new health hazards. Enhancement of the acknowledged public health infrastructure and intervention programmes is essential to reduce vulnerability to the health impacts of climate change. In the longer-term, fundamental improvements in the social and material conditions of life and in the reduction of inequalities within and between populations are required for sustained reduction in vulnerability to environmental health hazards.
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  • 82
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    Environmental monitoring and assessment 61 (2000), S. 37-48 
    ISSN: 1573-2959
    Keywords: adaptation ; climate change ; integration ; impacts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A mechanism has been established to improve integration of international climate-related programmes. Known as the Climate Agenda it outlines a programme, that in a cost-effective way, responds to national obligations to respond to international agreements as well as their national needs for social and economic development. The paper briefly describes the Climate Agenda and the incorporation within it of studies of climate impact assessments and response strategies to reduce vulnerability. The need for increased emphasis on climate impact assessment and for the development of effective adaptation measures is emphasised following the elaboration of a Kyoto Protocol to the United Nations Framework Convention on Climate Change (UNFCCC). UNEP's efforts to identify national programmes contributing to the Climate Agenda are described. The response from developing countries has, however, been very disappointing, even from countries where we know work is ongoing through funding by GEF or US Country Studies Program and other bilateral programmes. Initial compilation of information available so far shows that many developed countries are putting a lot into the area of impact assessment of not just climate change, but also climate variability. There remain some research gaps, especially in the area of assessment of climate impacts on ecosystems, hydrological systems, etc. Considerable efforts are presently being directed at reduction of emissions of greenhouse gases and in the case of developing countries, most efforts are being directed towards completing national communications and providing baseline data for future studies. The paper refers to early activities by UNEP in cooperation with other international organizations to undertake integrated assessments of the impacts of climate change on important socio-economic sectors and the later incorporation of lessons learned into the IPCC Guidelines for Assessing Impacts of Climate Change. Later sections outline the development of a handbook on methods for climate change impact assessment and adaptation strategies as a practical approach to national assessments and the development of appropriate and cost-effective response to climate change. The initiation of a GEF-funded project to apply the methods contained in the handbook and improve the results based on national studies is also described for both developed and developing countries. Working in collaboration with a team of international experts under the coordination of the Institute of Environmental Studies at Vrije University (Amsterdam), the goal of this ongoing project is to develop a valuable methodological tool that Parties to the UNFCCC may apply to develop national climate change impact and adaptation assessments. Development of these guidelines was linked to a series of country studies in Antigua and Barbuda, Estonia, Cameroon and Pakistan funded under a UNEP/GEF project. The application of the first version of the UNEP Handbook by national study teams in these four countries is making valuable technical and practical contributions and will ensure that the next version of the Handbook will be a more useful tool for experts in developing countries undertaking similar studies in the future. The methods contained in the Handbook are also the basis for similar assessments funded under bilateral development programmes in other countries. These and similar studies elsewhere are coordinated with the UNEP programme and will eventually aim to create reliable and comparable assessments, a compatible set of tools for such purpose and the identification of realistic adaptation options for incorporation into national planning for adapting to climate change. The paper also addresses how climate impact assessment and response strategies are undertaken as part of national enabling activities carried out in co-operation with UNEP.
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  • 83
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    Environmental monitoring and assessment 61 (2000), S. 113-122 
    ISSN: 1573-2959
    Keywords: Amazon ; Brazil ; deforestation ; carbon sink ; climate change ; climatic variability ; forest conservation ; habitat fragmentation ; logging ; tropical forests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This paper describes four global-change phenomena that are having major impacts on Amazonian forests. The first is accelerating deforestation and logging. Despite recent government initiatives to slow forest loss, deforestation rates in Brazilian Amazonia have increased from 1.1 million ha yr−1 in the early 1990s, to nearly 1.5 million ha yr−1 from 1992–1994, and to more than 1.9 million ha yr−1 from 1995–1998. Deforestation is also occurring rapidly in some other parts of the Amazon Basin, such as in Bolivia and Ecuador, while industrialized logging is increasing dramatically in the Guianas and central Amazonia. The second phenomenon is that patterns of forest loss and fragmentation are rapidly changing. In recent decades, large-scale deforestation has mainly occurred in the southern and eastern portions of the Amazon — in the Brazilian states of Pará, Maranhāo, Rondônia, Acre, and Mato Grosso, and in northern Bolivia. While rates of forest loss remain very high in these areas, the development of major new highways is providing direct conduits into the heart of the Amazon. If future trends follow past patterns, land-hungry settlers and loggers may largely bisect the forests of the Amazon Basin. The third phenomenon is that climatic variability is interacting with human land uses, creating additional impacts on forest ecosystems. The 1997/98 El Niño drought, for example, led to a major increase in forest burning, with wildfires raging out of control in the northern Amazonian state of Roraima and other locations. Logging operations, which create labyrinths of roads and tracks in forsts, are increasing fuel loads, desiccation and ignition sources in forest interiors. Forest fragmentation also increases fire susceptibility by creating dry, fire-prone forest edges. Finally, recent evidence suggests that intact Amazonian forests are a globally significant carbon sink, quite possibly caused by higher forest growth rates in response to increasing atmospheric CO2 fertilization. Evidence for a carbon sink comes from long-term forest mensuration plots, from whole-forest studies of carbon flux and from investigations of atmospheric CO2 and oxygen isotopes. Unfortunately, intact Amazonian forests are rapidly diminishing. Hence, not only is the destruction of these forests a major source of greenhouse gases, but it is reducing their intrinsic capacity to help buffer the rapid anthropogenic rise in CO2.
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    Environmental monitoring and assessment 61 (2000), S. 145-159 
    ISSN: 1573-2959
    Keywords: climate change ; adaptation ; economic development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Many developing countries, especially in Africa, contribute only very small amounts to the world total of greenhouse gas emissions. For them, the reduction of such emissions is not a priority, and the more important issue is to find ways to reduce their vulnerability to the projected climate change which is being imposed upon them largely as a result of emissions from developed countries. This priority does not accord with the ultimate objective of the United Nations Framework Convention on Climate Change, which is to achieve stabilization of greenhouse gas emissions. This paper reports upon studies in Uganda designed to help in the development of a national adaptation strategy, and addresses the need to reconcile such a strategy with the global priority accorded to mitigation and with national economic development priorities. Some features of a national climate change adaptation strategy are identified and questions are raised about the need for an international regime to facilitate and support adaptation.
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  • 85
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    Environmental monitoring and assessment 61 (2000), S. 193-205 
    ISSN: 1573-2959
    Keywords: human settlements ; climate change ; adaptation ; Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Climate change impacts on African human settlements arise from a number of climate change-related causes, notably sea level changes, impacts on water resources, extreme weather events, food security, increased health risks from vector home diseases, and temperature-related morbidity in urban environments. Some coastlines and river deltas of Africa have densely populated low-lying areas, which would be affected by a rise in sea level. Other coastal settlements will be subjected to increased coastal erosion. Recent flooding in East Africa highlighted the vulnerability of flood plain settlements and the need to develop adaptive strategies for extreme weather events management and mitigation. In the semi arid and arid zones many settlements are associated with inland drainage water sources. Increases in drought will enhance water supply related vulnerabilities. Inter-basin and international water transfers raise the need for adequate legal frameworks that ensure equity among participating nations. Similarly, water supply and irrigation reservoirs in seasonal river catchments might fail, leading to poor sanitation in urban areas as well as food shortage. Hydroelectric power generation could be restricted in drought periods, and where it is a major contributor to the energy budget, reduced power generation could lead to a multiplicity of other impacts. States are advised to develop other sources of renewable energy. Temperature changes will lead to altered distribution of disease vectors such as mosquitoes, making settlements currently free of vector borne diseases vulnerable. Rapid breeding of the housefly could create a menace associated with enteric disorders, especially in conditions of poor sanitation. The dry savannahs of Africa are projected as possible future food deficit areas. Recurrent crop failures would lead to transmigration into urban areas. Pastoralists are likely to undertake more trans-boundary migrations and probably come into conflict with settled communities. Adaptive measures will involve methods of coastal defences (where applicable), a critical review of the energy sector, both regionally and nationally, a rigorous adherence to city hygiene procedures, an informed agricultural industry that is capable of adapting to changing climate in terms of cropping strategies, and innovations in environment design to maximise human comfort at minimum energy expenditure. In the savannah and arid areas water resource management systems will be needed to optimise water resource use and interstate co-operation where such resources are shared. Climate change issues discussed here raise the need for state support for more research and education in impacts of climate change on human settlements in Africa.
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    Environmental monitoring and assessment 64 (2000), S. 213-225 
    ISSN: 1573-2959
    Keywords: biodiversity ; butterflies ; birds ; climate change ; montane vegetation ; remote sensing ; Greater Yellowstone Ecosystem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We used a time series of satellite multispectral imagery for mapping and monitoring six classes of montane meadows arrayed along a moisture gradient (from hydric to mesic to xeric). We hypothesized that mesic meadows would support the highest species diversity of plants, birds, and butterflies because they are more moderate environments. We also hypothesized that mesic meadows would exhibit the greatest seasonal and interannual variability in spectral response across years. Field sampling in each of the meadow types was conducted for plants, birds, and butterflies in 1997 and 1998. Mesic meadows supported the highest plant species diversity, but there was no significant difference in bird or butterfly species diversity among meadow types. These data show that it may be easier to detect significant differences in more species rich taxa (e.g., plants) than taxa that are represented by fewer species (e.g., butterflies and birds). Mesic meadows also showed the greatest seasonal and interannual variability in spectral response. Given the rich biodiversity of mesic montane meadows and their sensitivity to variations in temperature and moisture, they may be important to monitor in the context of environmental change
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    Aerobiologia 16 (2000), S. 331-334 
    ISSN: 1573-3025
    Keywords: allergenic pollen ; gene expression ; molecular biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The molecular mechanism of gene expression for pollen specificity is not yet fully known. However, it is an exciting area with great potential and has a wide scope of application in the field of molecular biology, breeding systems, biotechnology etc. The main aim of this write-up is to review some of the interesting achievements made through studies like gene expression in allergic pollen and the research which will make a way towards practical application of pollen molecular biology in identifying and isolating the genes responsible for all allergic disorders reported among various individuals.
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  • 88
    ISSN: 1573-0778
    Keywords: gene expression ; immortalised hepatocytes ; RAP-PCR ; RT-PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primary hepatocytes with SV40 DNA. In order toassess the suitability of immortalised lines as an alternativeto primary cell cultures we have used RNA arbitrarily primedpolymerase chain reaction to compare gene expression inimmortalised rat hepatocyte cell lines with that in primary rathepatocytes. We have found that differences exist in the RNAtranscripts between fresh and immortalised hepatocyteshighlighting RNA arbitrarily primed polymerase chain reaction asa useful screening method for identifying immortalised lineswhich retain the most `normal' phenotype in relation to theprimary cells from which they originated.
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  • 89
    ISSN: 1573-0778
    Keywords: cDNA ; differentiation ; gene expression ; growth ; humancells ; human tissues ; mitochondrial DNA ; mitochondrial RNA ; polyadenylated RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In the mitochondrion, essential genetic elements for replication and transcription are mostly housed within a shortsegment of its DNA located between tRNAPhe and tRNAPro genes, which is called mitochondrial regulatoryregion (mrr). RNAs are known to be transcribed from mrr, thestructures and the functions of which are yet to be fullycharacterized.We detected ca. 1.3 kb H-strand transcripts of mrr (mrrH-RNAs),and 0.2 kb L-strand transcripts of mrr (mrrL-RNAs) in varioushuman cultured cells and tissues using double stranded mrrDNAprobes. The steady state levels of mrrL-RNAs were generally highin cultured cells, while they varied among tissues. On the otherhand, the levels of mrrH-RNAs varied among tissues and amongcultured cells. A tendency was observed in these cells andtissues that a high level of mrrL-RNA is associated with cellproliferation, and a high level of mrrH-RNA withdifferentiation. Several cDNA clones to 1.3 kb mrrH-RNA were obtained from humanskeletal muscle polyadenylated RNAs. The 5′ terminus of the 1.3 kb RNA was determined to be at nucleotide position 15953 whichis immediately downstream of tRNAThr sequence.Polyadenylation site for most of the clones was demonstrated tobe at nucleotide position 576 which is immediately upstream oftRNAPhe sequence. The longest cDNA insert obtained was 1177 bps long spanning from nucleotide positions 15969 to 576 which could code for a peptide of 76 amino acids. The cDNAs isolatedhere are the first cDNA clones reported to human mrrH-RNAs.These results, together with previous results, furthersubstantiate that polyadenylated mrrH- and mrrL-RNAs are commonly present at varying levels among human tissues andcells. The 3′ end sequences of the cloned mrrH-cDNA provideswith insights into the mechanisms of transcription termination.The cDNA clones will provide tools to further the study of thefunction of mrr RNAs.
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    Studia geophysica et geodaetica 44 (2000), S. 57-72 
    ISSN: 1573-1626
    Keywords: extreme events ; heat wave ; tropical day ; climate change
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Physics
    Notes: Abstract Heat waves (periods of extremely hot summer weather) in the region of south Moravia are in the focus of this study. The introduced definition consists of three requirements imposed on the period that is considered a heat wave: at least three days with T MAX ≥30.0°C must be observed; the mean T MAX over the whole period is at least 30.0°C; and T MAX must not drop below 25.0°C. To compare the severity of the individual heat waves, various characteristics (duration, number of tropical days, peak temperature, cumulative temperature excess, precipitation amount) are examined. The heat wave index HWI is defined to express the severity of heat waves in the most comprehensive way. An extraordinary heat wave occurred in July and August 1994; it lasted more than a month at several stations, while the duration of a typical heat wave is only 4 - 7 days. The extremely long unbroken period of tropical days, and even of days with T MAX ≥32.0°C, represents the most distinct feature of the severe 1994 heat wave. With regard to heat wave characteristics, the summer temperature exceptionality of the early 1990s is indubitable.
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  • 91
    ISSN: 1573-9058
    Keywords: climate change ; pine forest ; Pinus sylvestris L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A closed CO2 and temperature-controlled, long-term chamber system has been developed and set up in a typical boreal forest of Scots pine (Pinus sylvestris L.) near the Mekrijärvi Research Station (62°47′N, 30°58′E, 145 m above sea level) belonging to the University of Joensuu, Finland. The main objectives of the experiment were to provide a means of assessing the medium to long-term effects of elevated atmospheric CO2 concentration (EC) and temperature (ET) on photosynthesis, respiration, growth, and biomass at the whole-tree level and to measure instantaneous whole-system CO2 exchange. The system consists of 16 chambers with individual facilities for controlling CO2 concentration, temperature, and the combination of the two. The chambers can provide a wide variety of climatic conditions that are similar to natural regimes. In this experiment the target CO2 concentration in the EC chambers was set at a fixed constant of 700 µmol mol−1 and the target air temperature in the ET chambers to track the ambient temperature but with a specified addition. Chamber performance was assessed on the base of recordings covering three consecutive years. The CO2 and temperature control in these closed chambers was in general accurate and reliable. CO2 concentration in the EC chambers was within 600–725 µmol mol−1 for 90 % of the exposure time during the "growing-season" (15 April – 15 September) and 625–725 µmol mol−1 for 88 % of the time in the "off-season" (16 September – 14 April), while temperatures in the chambers were within ±2.0 °C of the ambient or target temperature in the "growing season" and within ±3.0 °C in the "off season". There were still some significant chamber effects. Solar radiation in the chambers was reduced by 50–60 % for 82 % of the time in the "growing season" and 55–65 % for 78 % of the time in the "off season", and the relative humidity of the air was increased by 5–10 % for 72 % of the time in the "growing season" and 2–12 % for 91 % of the time in the "off season". The crown architecture and main phenophase of the trees were not modified significantly by enclosure in the chambers, but some physiological parameters changed significantly, e.g., the radiant energy-saturated photosynthesis rate, transpiration rate, maximum photochemical efficiency of photosystem 2, and chlorophyll content.
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  • 92
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    Water, air & soil pollution 123 (2000), S. 259-271 
    ISSN: 1573-2932
    Keywords: climate change ; greenhouse gases ; greenhouse gases in Israel ; inventory of emissions of greenhouse gases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract As a Party to the United Nations Framework Convention on Climate Change, Israel is committed to develop a national inventory of anthropogenic emissions and removals of greenhouse gases. This paper presents the national inventory, which was developed according to the guidelines of the Intergovernmental Panel on Climate Change (IPCC). The inventory includes the following sectors: energy, industrial processes, agriculture, forestry and waste. In this paper, only the inventory of the direct greenhouse gases (CO2, CH4 and N2O) is presented. Emissions of these gases were converted to CO2 equivalent emissions by means of their Global Warming Potentials (a measure of the radiative effects of the different gases relatively to CO2). CO2 emissions from burning fossil fuels to produce energy are by far the largest source (50 million tons in 1996). The contribution of methane emissions from decomposition of landfilled municipal solid waste is second in importance (8 million tons of CO2 equivalent). Industrial processes emit about 2 million tons CO2 equivalent, the most important process being cement production. Agricultural emissions amount to about 2 million tons CO2 equivalent and are due to soil emissions of nitrous oxide, methane emissions from enteric fermentation in domestic livestock and N2O and CH4 emissions from animal waste management. Although most forests in Israel are in a growing stage and atmospheric CO2 is therefore removed to form biomass, this removal amounts to 0.4 million tons only and is very small as compared to emissions from other sectors. On a per capita basis, Israel's emissions of CO2 from fuel combustion are not far behind those of some of the most developed countries.
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  • 93
    ISSN: 1573-1545
    Keywords: climate change ; technology policy ; uncertainty ; agent-based modeling ; exploratory modeling ; social interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Many governments use technology incentives as an important component of their greenhouse gas abatement strategies. These “carrots” are intended to encourage the initial diffusion of new, greenhouse-gas-emissions-reducing technologies, in contrast to carbon taxes and emissions trading which provide a “stick” designed to reduce emissions by increasing the price of high-emitting technologies for all users. Technology incentives appear attractive, but their record in practice is mixed and economic theory suggests that in the absence of market failures, they are inefficient compared to taxes and trading. This study uses an agent-based model of technology diffusion and exploratory modeling, a new technique for decision-making under conditions of extreme uncertainty, to examine the conditions under which technology incentives should be a key building block of robust climate change policies. We find that a combined strategy of carbon taxes and technology incentives, as opposed to carbon taxes alone, is the best approach to greenhouse gas emissions reductions if the social benefits of early adoption sufficiently exceed the private benefits. Such social benefits can occur when economic actors have a wide variety of cost/performance preferences for new technologies and either new technologies have increasing returns to scale or potential adopters can reduce their uncertainty about the performance of new technologies by querying the experience of other adopters. We find that if decision-makers hold even modest expectations that such social benefits are significant or that the impacts of climate change will turn out to be serious then technology incentive programs may be a promising hedge against the threat of climate change.
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    Bulletin of experimental biology and medicine 130 (2000), S. 898-899 
    ISSN: 1573-8221
    Keywords: peptides ; gene expression ; interleukin-2 ; lymphocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Lys-Glu in vitro stimulated interleukin-2 gene expression in mouse spleen lymphocytes. This effect depended on peptide concentration and duration of treatment. It is hypothesized that this peptide is the shortest regulatory fragment promoting the transport of trans-acting factors into the nucleus. It can not be excluded that Lys-Glu is a structural component of trans-acting factor active centers, which are necessary for the activation of interleukin-2 gene transcription in lymphocytes.
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    Bulletin of experimental biology and medicine 130 (2000), S. 1159-1161 
    ISSN: 1573-8221
    Keywords: erythroblasts ; cytokines ; gene expression ; hemopoietic stem cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Transplantation of erythroid and bone marrow cells to irradiated mice stimulated exogenous colony formation. Pretreatment of erythroid cells with specific rabbit antiserum to erythroblasts abolished this effect. The reverse transcriptase polymerase chain reaction revealed the presence of mRNA for interleukin-1α, interleukin-1β, interleukin-3, interleukin-6, and granulocyte-macrophage colony-stimulating factor in erythroid cells. Granulocyte-macrophage colony-stimulating factor was found in the conditioned medium from erythroid cells. Thus, erythroid cells stimulated colony-forming activity of bone marrow cells, which was probably mediated via cytokine synthesis (e.g., granulocyte-macrophage colony-stimulating factor).
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  • 96
    ISSN: 1573-8264
    Keywords: damaged trees ; stable isotopes ; water stress ; climate change ; lithologies ; water use efficiency ; nitrogen availability and use
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A severe drought that took place in Spain and other Mediterranean countries in 1994 produced a dieback of Quercus ilex trees on south-facing conglomerates but only minor defoliations in trees on north-facing schists. The foliar Δ13C of damaged trees continued to decrease in the next two wet years probably indicating increasing water use efficiency, and their δ15N continued to increase indicating progressive ecosystem N saturation and/or N losses whereas there were no significant changes in undamaged trees. Pre-drought Δ13C values were reached in the third year after the drought, but δ15N values did not yet recover. Another co-occurring small tree, Phyllyrea latifolia, did not show any damage and gained dominance in the most affected stands.
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    BioControl 45 (2000), S. 325-336 
    ISSN: 1573-8248
    Keywords: biological control ; climate change ; CO2 ; Bacillus thuringiensis var. aizawai
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Toxins from Bacillus thuringiensis have beenused as pest management tools for more than 50 years. The effect of these toxins depends on the quantityof Bacillus thuringiensis (Bt) toxins ingestedby susceptible insects. Food ingestion is affected byCO2 concentration; plants grown in elevatedCO2 often have increased carbon/nitrogen ratios(C/N), resulting in greater leaf area consumption. Therefore, we hypothesized that elevated CO2would improve the efficacy of foliar applications ofB. thuringiensis. Cotton plants were grown ateither ambient (360–380 μl/l) or elevated CO2(900 μl/l). Groups of plants in both CO2treatments were exposed to low (30 mg/kg soil/week) orhigh (130 mg/kg soil/week) nitrogen (N) fertilizationlevels in a split plot design. The resulting plantswere assessed for N and carbon (C) contents. Leafdisks from the same plants were dipped in a Btsolution and then fed to Spodoptera exigua(Hübner), an insect species of considerableeconomic importance. Elevated CO2 significantlyreduced total N, and increased the C/N. Nitrogenfertilization significantly affected consumption byearly stadia larvae, larval weight gain, and relativegrowth rate (RGR). Interactions between CO2concentration and N fertilization level significantlyimpacted late stadia larval food consumption, andthrough differential Bt toxin intake, affectedduration of larval stage and mortality to the adultstage. We conclude that the elevated atmosphericCO2 concentrations expected in the next centurywill interact with commercial fertilization practicesto enhance the efficacy of B. thuringiensisformulations applied topically to crops. Theimplications for improved control are discussed.
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    Biologia plantarum 43 (2000), S. 481-489 
    ISSN: 1573-8264
    Keywords: cell symmetry ; gene expression ; heat shock proteins ; polarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenesis can be initiated directly from microspores or pollen grains. This is known as androgenesis and refers to the process of redirection of normal pollen development (gametophytic pathway) towards the embryo formation (sporophytic). This review mainly deals with the current knowledge of stress and developmental aspects of induction of androgenesis. The crucial role of stress inductive treatment together with changes in cell polarity are discussed in relation to other relevant biological systems. The intriguing speculations are made on the basis of these comparisons which may point out the direction of future investigations.
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