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  • Artikel  (11)
  • monoclonal antibody  (7)
  • Lepidoptera
  • temperature
  • Springer  (11)
  • 2000-2004  (7)
  • 1985-1989  (4)
  • Werkstoffwissenschaften, Fertigungsverfahren, Fertigung  (11)
  • 1
    Digitale Medien
    Digitale Medien
    High density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multi-settling zones to separate cells from the culture medium (1989)
    Springer
    Cytotechnology 2 (1989), S. 5-8 
    Details
    ISSN: 1573-0778
    Schlagwort(e): hybridoma ; monoclonal antibody ; perfusion culture ; mammalian cell culture ; serum-free culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Mouse-human hybridoma X87X cells were cultivated using a novel perfusion culture apparatus provided with three-settling zones to separate the cells from the culture medium by gravitational settling. The maximum viable cell density in a serum-free culture medium attained 3.0×107 cells/ml, when the specific perfusion rate was set to 2.3 vol day-1, and monoclonal antibody was continuously produced. These results were almost the same as those in the perfusion culture vessel with one settling zone and revealed that the process with a plurality of settling zones is a promising one for scale-up of a gravitation type of perfusion culture vessel.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365409
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  • 2
    Digitale Medien
    Digitale Medien
    SSGF-I, a potent growth-promoting substance for mammalian cells from swine serum (1989)
    Springer
    Cytotechnology 2 (1989), S. 9-17 
    Details
    ISSN: 1573-0778
    Schlagwort(e): heterohybridoma ; LDL ; monoclonal antibody ; serum-free medium ; PEG ; swine serum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A small amount of swine serum markedly stimulated cell growth for high productivity subclones derived from a mouse human-human heterohybridoma, N12-16.63, secreting an anti-tetanus toxoid human monoclonal antibody in a polyethylene glycol (PEG)-containing serum-free medium, PEG-86-1. A growth promoting substance, SSGF-I, was isolated from the serum by ammonium sulfate fractionation, Cibacron blue F3A-G affinity chromatography, DEAE-agarose ion exchange chromatography, and gel filtrations on Trisacryl GF 2000 and Sephacryl S-300. SSGF-I was characterized as a low density lipoprotein (LDL) of swine serum by its physico-chemical properties. It promoted cell growth synergistically with PEG and its optimum concentration was 1 to 100μg/ml. Human LDL was less active, and human or swine high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were inactive. Based on these results, we propose an improved serum-free medium, PEG-86-3, which contains all the ingredients of PEG-86-1 and 10μg/ml SSGF-I. This medium is useful for not only high productivity heterohybridomas but also for a variety of lymphoid cell lines.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00365410
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  • 3
    Digitale Medien
    Digitale Medien
    Egg yolk lipoprotein, a new supplement for the growth of mammalian cells in serum-free medium (1988)
    Springer
    Cytotechnology 1 (1988), S. 159-169 
    Details
    ISSN: 1573-0778
    Schlagwort(e): lipoprotein ; egg yolk ; serum-free medium ; cell growth ; monoclonal antibody ; ITES
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Egg yolk lipoprotein promoted growth of a wide variety of mammalian cell lines, including plasma-cytomas and epithelial cell lines, in serum-free medium. The lipoprotein was active for cell growth when used with insulin, transferrin, ethanolamine and selenite. The most active lipoprotein fraction (YLP-pI7.5) was purified to give a single peak by chromatofocusing and gel filtration, and was homogeneous on a 0.35% agarose gel electrophoretogram. The lipoprotein was characterised as a very low density lipoprotein with a protein content of only 1.3%. This lipoprotein had an optimal concentration of 300 μg/ml (4 μg protein/ml). It was easily separable from proteinous molecules secreted into the serum-free medium by the cells, since it floated on the surface of the medium after addition of ammonium sulfate, to precipitate protein, and centrifugation. An associated structure of lipid and protein seemed to be still necessary for the lipoprotein to exhibit a growth promoting activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00146817
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  • 4
    Digitale Medien
    Digitale Medien
    High cell density perfusion culture of hybridoma cells recycling high molecular weight components (1988)
    Springer
    Cytotechnology 1 (1988), S. 171-178 
    Details
    ISSN: 1573-0778
    Schlagwort(e): high-density ; hybridoma ; monoclonal antibody ; perfusion culture ; serum-free ; suspension culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media. The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00146818
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  • 5
    Digitale Medien
    Digitale Medien
    Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization. Monoclonal antibody for a ginseng saponin (2000)
    Springer
    Cytotechnology 34 (2000), S. 197-204 
    Details
    ISSN: 1573-0778
    Schlagwort(e): ELISA ; ginsenoside Rg1 ; mass spectrometry ; monoclonal antibody ; qualitative analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The ratio of hapten and bovine serum albumin in an antigenconjugate was determined by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry. Ahybridoma secreting monoclonal antibody against ginsenosideRg1 was produced by fusing sprenocytes immunized with aginsenoside Rg1-bovine serum albumin conjugate withHAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very smallcross-reaction appeared with ginsenoside Re. The full measuringrange of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 ofginsenoside Rg1.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008162703957
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  • 6
    Digitale Medien
    Digitale Medien
    Studies on anaerobic proteolytic bacteria of Leh, India (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 297-301 
    Details
    ISSN: 1573-0972
    Schlagwort(e): Anaerobic bacteria ; growth ; protease ; psychrotrophs ; temperature ; volatile fatty acids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Five anaerobic proteolytic bacteria were isolated from water bodies of Leh, India, where the ambient temperature varies from −25 to 25 °C. Isolates showed growth at all temperatures ranging from 5 to 37 °C except SPL-4 and SPL-5 which showed no growth at 5 °C. The cultures could grow and produce proteases on various protein substrates and the yield varied with the substrates. Two of the cultures showed the presence of spores. Acetate was the dominant VFA during hydrolysis of protein substrates.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008935425175
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  • 7
    Digitale Medien
    Digitale Medien
    Rubber stoppers used with sulphidic media: a note of caution (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 571-572 
    Details
    ISSN: 1573-0972
    Schlagwort(e): Anaerobes ; hydrogen sulphide ; rubber stoppers ; sulphate reduction ; temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Common black rubber stoppers, made from natural rubber and styrene–butadiene, may cause a loss of hydrogen sulphide from aqueous media and impede the growth of sulphate-reducing bacteria under thermophilic conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008909530445
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  • 8
    Digitale Medien
    Digitale Medien
    Effects of temperature, water activity and gas atmosphere on mycelial growth of tempe fungi Rhizopus microsporus var. microsporus and R. microsporus var. oligosporus (2000)
    Springer
    World journal of microbiology and biotechnology 16 (2000), S. 853-858 
    Details
    ISSN: 1573-0972
    Schlagwort(e): Carbondioxide ; fungi ; oxygen ; Rhizopus ; solid-substrate fermentation SSF ; tempe modelling ; temperature ; water activity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Rhizopus microsporus var. microsporus and var. oligosporus are used in the manufacture of various Asian fermented foods (tempe, black oncom, sufu). In view of solid-substrate fermentation (SSF) control, mycelial growth of strains of both varieties was tested for sensitivity to fluctuations of temperature, water activity and interstitial gas composition. This was achieved by measuring radial growth as well as biomass dry weight of pre-germinated microcolonies on defined media. The optimum conditions were temperature 40 °C, a w 0.995 and a gas composition of air for the growth of both strains on a model medium. Whereas radial growth rates of var. microsporus and var. oligosporus were similar, biomass growth rates of var. oligosporus were higher than those of var. microsporus under optimum conditions. The temperature-dependent growth of Rhizopus spp. at a w 〉 0.98 could be described by the Ratkowsky Equation. Carbon dioxide (5–10% v/v) inhibited the growth of Rhizopus spp. at non-limiting levels of oxygen. The two strains were able to grow at low (0.5% v/v) oxygen levels, but the mycelial density was rather low. No interrelation of water activity and gas composition was observed, but at high water activity the fungi were more sensitive to changes of temperature. The implications for process control are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008974621698
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  • 9
    Digitale Medien
    Digitale Medien
    Construction of recombinant monoclonal antibodies from a chicken hybridoma line secreting specific antibody (2000)
    Springer
    Cytotechnology 32 (2000), S. 191-198 
    Details
    ISSN: 1573-0778
    Schlagwort(e): chicken ; immunoglobulin gene ; monoclonal antibody ; phage display ; prion protein ; recombinant antibody
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract The chicken is a useful animal for the development of the specificantibodies against the mammalian conserved proteins. We generated twotypes of recombinant chicken monoclonal antibodies (mAbs), using a phagedisplay technique from a chicken hybridoma HUC2-13 which secreted themAb to the N-terminal of the mammalian prion protein (PrP). Althoughthe mAb HUC2-13 is a useful antibody for the prion research, thehybridoma produces a low level of antibody production. In order to producea large amount of the mAb, we have constructed a single chain fragmentvariable region (scFV) mAb by using the variable heavy(VH) and light (VL)genes which were amplified by using the two primer pairs and theflexible linker. The two phage display mAbs (HUC2p3 and HUC2p5)expressed on a M13 filamentous phage and their soluble type mAbs(HUC2s3 and HUC2s5) were reacted with the PrP peptide antigen in theELISA. In the Western blot analysis, the mAbs HUC2p3 and HUC2s3 wereas reactive to PrPc from mouse brains as the mAb HUC2-13 was. The nucleotide sequences of VH and VL genes from HUC2-13 and the two cloneswere identical except for only one residue. These results indicate that themethods presented here provide an effective tool for the improvement ofthe low levels of antibody production in the chicken hybridoma system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008149815908
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  • 10
    Digitale Medien
    Digitale Medien
    Effect of feed rate on growth rate and antibody production in the fed-batch culture of murine hybridoma cells (2000)
    Springer
    Cytotechnology 32 (2000), S. 229-242 
    Details
    ISSN: 1573-0778
    Schlagwort(e): fed batch ; hybridoma ; macromolecular composition ; monoclonal antibody ; substrate limitation ; target specific growth rate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Batch and fed-batch cultures of a murine hybridomacell line (AFP-27) were performed in a stirred tankreactor to estimate the effect of feed rate on growthrate, macromolecular metabolism and antibodyproduction. Macromolecular composition was foundto change dynamically during batch culture ofhybridoma cells possibly due to active production ofDNA, RNA and protein during the exponential phase.Antibody synthesis is expected to compete with theproduction of cellular proteins from the amino acidpool. Therefore, it is necessary to examine therelationship between cell growth in terms of cellularmacromolecules and antibody production. In this study,we searched for an optimum feeding strategy bychanging the target specific growth rate in fed-batchculture to give higher antibody productivity whileexamining the macromolecular composition. Concentratedglucose (60 mM) and glutamine (20 mM) in DR medium(1:1 mixture of DMEM and RPMI) with additional aminoacids were fed continuously to the culture and thefeed rate was updated after every sampling to ensureexponential feeding (or approximately constantspecific growth rate). Specific antibody productionrate was found to be significantly increased in thefed-batch cultures at the near-zero specific growthrate in which the productions of cellular DNA, RNA,protein and polysaccharide were strictly limited byslow feeding of glucose, glutamine and other nutrients. Possible implications of these results are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008169417980
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