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  • Articles  (71)
  • Wiley-Blackwell  (47)
  • De Gruyter  (24)
  • Annual Reviews
  • 2005-2009  (24)
  • 1980-1984  (47)
  • Natural Sciences in General  (71)
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  • Articles  (71)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 83-94 
    ISSN: 0741-0581
    Keywords: Scanning transmission electron microscopy ; Image contrast ; Inelastic scattering ; Thick specimens ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: For scanning transmission electron microscopy (STEM) images obtained with relatively small objective aperture sizes, the contrast of small objects contained within thick specimens may be considerably enhanced by using an off-axis detector aperture situated on the edge of the central beam spot. The effect is demonstrated for both crystalline and amorphous specimens. The effect arises because the detector collects part of the small angle inelastic scattering and is modified by refraction effects for specimens of rapidly changing thickness.
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 107-130 
    ISSN: 0741-0581
    Keywords: Phase contrast ; Computer simulation ; Partial coherence ; Electron microscopy ; Convergent beam ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A general method for computing high-resolution conventional transmission electron microscope images and diffraction patterns, when there are different types of partially coherent illumination conditions, is described. Examples of convergent beam, hollow cone, and virtual aperture illumination conditions are given in the context of interpreting image features. A comparison of real and computed diffraction patterns shows that, in practice, many innovative imaging modes are possible, which can be verified prior to real microscope experiments.
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 175-184 
    ISSN: 0741-0581
    Keywords: Synchronous digital image acquisition and scan generation (SDIASG) ; X-ray imaging ; Scanning transmission electron microscope ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: An intelligent interface has been designed to perform synchronous digital image acquistion and scan generation (SDIASG interface) for a microprocessor controlled Scanning Transmission Electron Microscope (S(T)EM) with x-ray imaging. The SDIASG interface connects an LSI-11/2 microprocessor to a Philips EM400 electron microscope. The LSI-11/2 microprocessor is part of a DeAnza VC5000 digital image display system. A system using the SDIASG interface is described. The system takes advantage of the SDIASG interface and a DeAnza VC5000 digital image display system to realize new capabilities that optimize conditions for x-ray mapping.A low characteristic x-ray count rate is generated by the ultrathin specimens from which high resolution x-ray maps can be obtained (Shuman et al, 1976; Somlyo and Shuman, 1982). This low count rate necessitates a long image accumulation time, which in turn makes drift correction essential for maintaining spatial resolution. The new capabilities of the system described here consist of real-time display and summation of consecutive image and x-ray maps, and automatic return to a high speed imaging mode between consecutive x-ray map passes. The new capabilities combine to allow frequent correction for specimen drift between consecutive x-ray mapping passes while still permitting a long total accumulation time for the x-ray maps.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 331-340 
    ISSN: 0741-0581
    Keywords: Digital image processing ; Laplacin filter ; Scanning electron microscopy ; High-resolution ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Certain digital image-processing methods, which are useful for nonperiodic structural images, have been applied to high-resolution SEM images for the improvement of resolution. Samples utilized in the present study consisted of magnetic tape coated with gold, T4 phage coated with gold-palladium, and uncoated specimens of Prolamellar body (PLB) in Cucurbita moschata. These images were blurred and otherwise disturbed by electronic noise, though the images were taken at the limit of efficiency of intrinsic instrument. The major image-processing tool was the Laplacian filter, which subtracts the Laplacian from the original image. Noise, which is a serious problem in digital processing of high-resolution SEM images, was suppressed by the nonlinear type smoothing method. Also, the noise was evaluated by an autocorrelation function and a power spectrum of the image. By using these methods of “deblurring” and noise removal, we achieved better resolution, and structural details of our biological specimens were revealed.
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 131-140 
    ISSN: 0741-0581
    Keywords: GACH ; Amino-resin ; SEM ; Preparation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Biological specimens can be prepared for scanning electron microscopy by means of copolymerizing the fixing agent glutaraldehyde with carbohydrazide prior to air drying. Such preparations are more stable in the electron microscope, show less internal cellular disruption and retain more of their native elemental composition than specimens prepared by means of dehydration and critical-point drying. Specimens observed in the scanning electron microscope can often be recovered for thin sectioning with no additional embedment, and can then be observed by means of transmission elecltron microscopy. The preparation (termed GACH) can be performed in almost any laboratory with no specialized equipment and, for the most part, may be carried out at room temperature. The technique appears to provide the promise of further research applications in scanning electron microscopy which may employ conjugated procedures of immunocytochemistry and cathodoluminescence as well as X-ray microanalysis in limited situations.
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  • 6
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 203-204 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 243-270 
    ISSN: 0741-0581
    Keywords: Immunocytochemistry ; Protein A-Gold ; Lowicryl ; Glycolmethacrylate ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The postembedding protein A-gold immunocytochemical approach has been introduced as an alternative to other techniques for the ultrastructural localization of antigenic sites. The present review deals with the development, the theoretical background, and technical approach of the protein A-gold method as well as the different modifications introduced in order to enhance the resolution of the results and to perform double labelings on the same section. Various examples demonstrate the reliability and the wide range of application of this technique. In addition, some problems, pitfalls, and limitations particular to this method are reported.
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  • 8
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 271-277 
    ISSN: 0741-0581
    Keywords: Vascular cell cultures ; Transmission electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A method is described for obtaining optimal, reproducible ultrastructure of vascular smooth muscle cells and vascular endothelial cells in culture. Routinely grown cultures are prepared for TEM with a precise regimen of fixation, postfixation, en bloc staining, dehydration, and embedment. The most important aspects of this procedure are the following: (1) fixation with a percentage-gradient series of glutaraldehyde solutions at 37°C, (2) immediate postfixation with osmium tetroxide solution, and (3) block-staining with uranyl acetate solution to eliminate any extraction of constituents during subsequent processing.
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  • 9
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 289-298 
    ISSN: 0741-0581
    Keywords: Epithelial cell ; Membrane ; Ecto-ATPase ; Stain-replica ; Plasma polymerization ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A stain-replica technique is described for cytochemical examination of ecto-adenosine triphosphatase (ATPase) activity over the membrane surface of monolayer cell cultures. Rat liver epithelial cells grown on a plastic substrate were fixed in glutaraldehyde, incubated in situ in an ATPase-lead reaction medium, ethanol-dehydrated and air-dried. The cell surface of the monolayer cultures was replicated with plasma polymerization of hydrocarbon gas in the negative phase of glow discharge. X-ray microprobe analysis confirmed the site-specific deposition of lead phosphate in the polymer-replica films. The cytochemical localization of lead was mirrored in the replicas of epithelial cells, demonstrating that ATPase activity was expressed along the apical margins of cell-to-cell contacts. Little or no activity was present over the remainder of the smooth-surface membranes. In transformed epithelial cells, there were abundant reaction products over the microvilli and intercellular boundaries. These observations were consistent with biochemical data on the liver epithelial cells in culture and suggested the potential of surface-replica cytochemistry.
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  • 10
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 373-385 
    ISSN: 0741-0581
    Keywords: TEM ; Parallax equation ; Freeze-etch ; Pt-C replication ; Hydrated spermidine-condensed DNA toruses ; Stereoheight measurements ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Stereoimaging of hydrated single complex macromolecules requires thin freeze-etch platinum-carbon replicas (≤200 Å) and that the transmission electron microscope (TEM) be equipped with a tilt-rotation eucentric goniometer stage. The original parallax equation is an accurate approximation for high-magnification work, micrographs (105 ×) being less than 0.3% in error. In addition, we have derived formulas for high-magnification work to measure heights, lateral distances, and the object tilt angle for an object not lying flat on the film surface. The accuracy of the height measurements is evaluated on spermidine-condensed DNA toruses. By using the maximum error equation derived from the original parallax equation, we discuss methods to improve the height measurement precision (95% fractile) to the 5-10 Å range.
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  • 11
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 417-418 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 12
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 419-420 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 13
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 1-7 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 14
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 53-61 
    ISSN: 0741-0581
    Keywords: Cross-section specimen ; Thin films ; Interfaces ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The structure and chemistry of thin solid films are best studied by transmission electron microscopy (TEM) when they are viewed in cross-section - that is, when the surface normal of the film is made perpendicular to the electron beam. In this orientation, the substrate, the thin film layers, and the interfaces between them can be imaged either simultaneously or individually. Further, information from each of these regions remains distinct from that obtained from the others, eliminating the problems of superimposition that are a consequence of viewing a layered structure in the conventional manner (i.e., parallel to the surface normal). A technique for fabricating TEM specimens that can be viewed in cross-section is described here. Although the majority of our work is with silicon-based materials, the technique can be readily adapted to the study of other systems.
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  • 15
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 313-314 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 16
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 299-309 
    ISSN: 0741-0581
    Keywords: Electron microscopy ; Ion microscopy ; Correlative microscopy ; Electron probe microanalysis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, we have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number 〉 9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest. After sputter-coating the sample with platinum, metal-free shadows are visible using a low-power reflected light microscope available on a typical IM sample chamber as a guide for ion beam placement. The conductive coatings also minimize specimen charging during IM. Post-IM light microscopy, SEI, and BEI are used to confirm the location of specific areas probed in the IM experiments and to provide information on differential ion-sputtering artifacts and tissue contaminants. This new correlative technique should permit better understanding of the images obtained with these diverse instruments.
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  • 17
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 387-398 
    ISSN: 0741-0581
    Keywords: Ultramicrotomy ; Serial sectioning ; Electronmicroscopy ; Seria reconstruction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The process of serial sectioning for electron microscopy has been refined such that loss of thin sections is kept below 0.1% and the series is continued at will. The method relies on microscopic control of all manipulative steps, Formvar casting on plate glass for coated slot grids, coating of the block with contact cement for reliable ribboning, pickup by a one-step method with grid support in the diamond knife trough, staining in LKB grid holders, gentle treatment of grids in the electron microscope, and a slight modification to the microscope for safe grid withdrawal. The results are particularly applicable to the reconstruction of neuronal microcircuits and larger volumes of neuropil.
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  • 18
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 405-414 
    ISSN: 0741-0581
    Keywords: Ceramics ; Electron microscopy ; Ion milling ; Specimen preparation ; Sputtering ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Ion bombardment to perforation is a common technique in the materials sciences by which thin specimens can be prepared for transmission electron microscopy. The process is not without complication and involves radiation damage to the specimen and tends not to preserve the initial specimen topology. Some of the more important facets of the ion-milling process, pertinent to such specimen preparations, are described.
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  • 19
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    Journal of Electron Microscopy Technique 1 (1984), S. 9-29 
    ISSN: 0741-0581
    Keywords: Quick freezing ; Synaptic vesicles ; Cholinergic nerve terminals ; Electric organ ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The limitations of chemical fixation in permitting the 1:1 quantitative correlations required for convincing ultrastructural explanations of cell biological processes are noted. We describe techniques for obtaining highly reproducible direct quick freezing on the polished surface of pure copper bars dipping into a static dewar of liquid N2. The importance and the ease of testing and obtaining bounce suppression with commerically available equipment is emphasized. Artefacts caused by tissue damage and bad freezing are illustrated, and a hitherto unrecognized population of presynaptic membrane attached vesicles is described in Torpedine electric organ. Between 15 and 20% of the synaptic vesicles are attached to ca. 30% of the cytoplasmic face of the presynaptic terminal membrane. There is a close correlation between the occurrence of such attachments and the application of electrocyte basal lamina to the external face. We suggest that these vesicles are the ‘membrane operators,’ ‘vesigates,’ and ‘highly active subpopulation’ of vesicles whose existence has been invoked to explain biochemical data in other laboratories. We further speculate that relatively selective Ca pumping by this immediately submembranous population leads to displacement of acetylcholine (ACh) and reloading with newly synthesized ACh. The preferential release of the latter would then be expected.
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  • 20
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 95-96 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 21
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 63-81 
    ISSN: 0741-0581
    Keywords: Autoradiography ; Mask analysis ; Neuromuscular junction ; Acetylcholine receptor ; Junctional folds ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Several methods of analyzing EM autoradiograms are now available. Two such procedures, the grain density distribution (or histogram) method and the mask method use the resolution of the EM autoradiographic technique to generate grain distributions expected from postulated sources, and compare these with the observed grains in the autoradiograms. These two methods are here compared in the analysis of label on linear sources: the distribution of labeled acetylcholine receptor (AChR) down the postjunctional folds of lizard and frog neuromuscular junctions. The receptors were labeled with I-25-α-bungarotoxin and the autoradiograms coated with the high resolution Kodak emulsion 129-01. We found that both methods gave similar results in confirming that the bulk of the AChR is concentrated on the thickened region of the membrane at the top ∼2000 A of the junctional folds, and that there may be a gradient of receptor concentration down the folds. The grain density distribution method is simpler, but does not lend itself easily to quantifying the extent of deviation from simple models. Although computer graphics is not necessary for either method, its use allows the expected grains from linear sources to be generated quickly, making the mask analysis a feasible routine method for assigning the extent of label in different membrane regions.
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  • 22
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    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 1 (1984), S. 141-150 
    ISSN: 0741-0581
    Keywords: Electron microprobe ; X-ray analysis ; Kidney physiology ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The present investigation describes a modification of the liquid droplet technique that allows for the quantitative elemental analysis of small volumes (〈 100 picoliters) of aqueous biologic samples using a scanning transmission electron microscope (Philips 400 HTG-STEM) equipped with an EDAX energy dispersive detector. Aliquots of samples and standards were micropipetted onto solid beryllium supports under paraffin oil. The oil was washed with organic solvents and the samples frozen and freeze-dried. The samples were excited in a Philips 400-HTG-STEM by scanning a 1-μm, 20-kV electron beam over the surface of the droplets, and the X-ray spectra were collected. Measured X-ray intensities in characteristic peaks were found to be linearly related to the concentration of various elements in the sample. This work demonstrates the feasibility of performing quantitative elemental analysis of minute samples and cells in a scanning transmission electron microscope equipped with an energy dispersive X-ray detector.
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  • 23
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    Journal of Electron Microscopy Technique 1 (1984), S. 199-201 
    ISSN: 0741-0581
    Keywords: Critical point drying ; Electron microscopy ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The principles and methods for constructing an improved chamber for dehydration and critical point drying of multiple biological samples are described. The specimen chamber design is based on vertical positioning of the electron microscope grids or coverslips and permits minimal perturbation of laminar solvent flow past the specimens. This condition is requisite for optimal exposure of samples to solvents, which is necessary for complete dehydration and drying. Fragile samples, including chromosomes, critical point dried in the multisample chamber demonstrate crisp, well-preserved, three-dimensional morphology.
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  • 24
    ISSN: 0741-0581
    Keywords: Glomerular capillary endothelium ; Vascular perfusion ; Freeze-cracking ; Scanning electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Modern morphological investigation requires the use of a variety of technological approaches and the employment of rigorous morphometric analysis for an adequate evaluation of the structural and ultrastructural features of a tissue or organ. The introduction of the technique of freeze-cracking of tissue to expose new surfaces has made it possible to quantitate the normal surface characteristics of the glomerular capillaries of the mammalian kidney. This report describes the techniques used for the preparation and quantitative assessment of normal glomerular endothelial morphology. The techniques of in vivo and in vitro vascular perfusion of kidneys as a method of fixation and the freeze-cracking of tissue are outlined in detail. In addition, a morphometric analysis of the endothelial surface characteristics are described and values are reported for the control rat and human kidneys from transplant donors.
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  • 25
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    Journal of Electron Microscopy Technique 1 (1984), S. 205-206 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 26
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    Journal of Electron Microscopy Technique 1 (1984), S. 219-225 
    ISSN: 0741-0581
    Keywords: Monolayer cells ; preparation for SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Monolayers of PtK-1 and HeLa cells grown on glass or plastic supports are extremely susceptible to lacerations, e.g., splits and cracks caused mainly by shrinkage when prepared for scanning electron microscopy (SEM). We find that a four-step fixation procedure including glutaraldehyde, OsO4, tannic acid, and uranylacetate application, in combination with critical point drying, drastically reduces these structural damages. In addition, the conductivity of the specimens is enhanced, so that they can be investigated without gold coating. Transmission electron microscopy (TEM) investigation of perpendicular sections in the area of lacerations provides evidence that the subcortical cytoskeletal elements are of crucial importance in maintaining cell membrane stability during the preparations. Our relatively quick and simple procedure results in an improved structural appearance of the cells.
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  • 27
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    Journal of Electron Microscopy Technique 1 (1984), S. 279-284 
    ISSN: 0741-0581
    Keywords: Electron diffraction ; Zone-axis patterns ; Convergent-beam diffraction ; Tanaka method ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The “Tanaka” method is one of several techniques that make it possible to obtain zone-axis electron diffraction patterns in a transmission electron microscope without the restriction in the field of view that limits normal convergent-beam diffraction patterns.The method employs a convergent-beam of electrons focused to a probe in a plane that does not coincide with the specimen. The selected area aperture can then be used to eliminate all but one of the diffracted beams to obtain the desired pattern. Practical details of operation and values of operating parameters are discussed.The Tanaka method is a useful addition to the techniques available to the electron microscopist, especially since no instrumental modification is required.
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  • 28
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    Journal of Electron Microscopy Technique 1 (1984), S. 311-312 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 29
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    Journal of Electron Microscopy Technique 1 (1984), S. 315-316 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 30
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    Journal of Electron Microscopy Technique 1 (1984) 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 31
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    Journal of Electron Microscopy Technique 1 (1984), S. 349-372 
    ISSN: 0741-0581
    Keywords: Enzyme-gold ; Cytochemistry ; Nucleic acids ; Elastin ; Collagen ; Glycogen ; Xylans ; Chitins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The enzyme-gold postembedding approach has been introduced recently in the field of cytochemistry for the ultrastructural localization of macromolecules. This technique is based on the affinity properties existing between an enzyme and its substrate. The possibility of detecting substrate molecules by applying enzyme-gold complexes has been established. The present review deals with the development and the technical approach of this method. Various applications are reported for the demonstration of the reliability of the technique that yields results of high specificity and resolution. In addition, some technical problems and limitations particular to this method are reported and discussed.
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    Journal of Electron Microscopy Technique 1 (1984), S. 399-404 
    ISSN: 0741-0581
    Keywords: Particle size ; Electron microscopy ; Microcomputer programs ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A formula is derived to enable the calculation of the true height of an object, such as a shadowed latex bead, from electron micrographs. Knowing only the angle of shadowing and the length of the evaporated shadow, and by substituting these values in the derived formula, a microcomputer may be programmed to carry out the necessary computations. An example of such a microcomputer program is given. The correct determination of the height of particles by electron microscopy using the shadowing technique is one of the most accurate methods available for the determination of small particle height.
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  • 33
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    Journal of Electron Microscopy Technique 1 (1984), S. 415-416 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 34
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    Journal of Electron Microscopy Technique 1 (1984), S. 209-209 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 35
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    Journal of Electron Microscopy Technique 1 (1984) 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 36
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    Journal of Electron Microscopy Technique 1 (1984), S. 227-241 
    ISSN: 0741-0581
    Keywords: PEG method ; resinless section ; microtrabeculae ; cytoplasmic sol et gel ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A simple and reliable method to make resinless sections for electron microscopy was recently developed by using polyethylene glycol (PEG) as a transient embedding media. In this paper the practical procedure of this PEG method is described in detail. Normal ultrastructure of several types of in-situ cells in resinless sections is demonstrated. The cytoplasmic matrix of all in-situ cells examined is revealed to consist of the microtrabecular lattice. A result from application of this technique to immuno-electron microscopy is also illustrated. This method is shown to have potential in overcoming the problem of intracellular penetration of macromolecular antibodies. Several artifacts caused by failures in specimen preparations are displayed. The real or artifactual nature of the microtrabecula is briefly discussed.
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  • 37
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    Journal of Electron Microscopy Technique 1 (1984), S. 285-287 
    ISSN: 0741-0581
    Keywords: SEM ; Coal analysis ; Mounting medium ; Polished sections ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A high electron-density embedding medium was developed for SEM observation of inorganic constituents of organic or carbonaceous particles. The components used are a common epoxy resin in which iodoform is dissolved before the addition of the hardener. An iodoform content of 10% by weight proved satisfactory for obtaining excellent contrast between the matrix and embedded carbonaceous particles in the SEM. The system has been successfully applied in the preparation of polished specimens of coal particles. There is no interference between the iodine and any of the most abundant or most important coal mineral components, but it was found that the epoxy resin contained chlorine as a contaminant.
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  • 38
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    Journal of Electron Microscopy Technique 1 (1984) 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 39
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    Journal of Electron Microscopy Technique 1 (1984), S. 207-208 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 40
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    Journal of Electron Microscopy Technique 1 (1984), S. 31-35 
    ISSN: 0741-0581
    Keywords: Photography ; Point source enlarger ; Electron micrograph ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Point source enlargers may cause unusual types of printing defects. One type is a large spot in the center of the enlarged picture field that sometimes appears when the edges of negatives are not adequately masked during printing. Another type is a blurry image caused by a defect in the polycontrast filter. The defect appears in the filter as a small spot of about 1/8-inch diameter, formed, presumably, by heat from the focused beam of the point source light. A spot defect of this type is difficult to see by a cursory visual examination of the filter and may develop unnoticed and persist for months before it is finally recognized.
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  • 41
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    Journal of Electron Microscopy Technique 1 (1984), S. 151-174 
    ISSN: 0741-0581
    Keywords: Cardiac muscle ; Rapid freezing ; Cryosectioning ; X-ray microanalysis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Electrically stimulated heart muscle preparations can be quickly frozen in undercooled propane at defined times of the mechanically controlled contraction cycle. The apparatus for triggered freezing of the muscle strips in undercooled propane is described in detail. Freeze substitution of some strips after freezing shows the degree of ice crystal formation without the potential interference of artifacts introduced later by cryosectioning and freeze drying. Ultrathin longitudinal and transversal cryosections are cut with a LKB cryoultramicrotome at temperatures of -130 to -140°C, freeze-dried at 10-6 Torr vacuum and carbon-coated before analysis. The freeze-dried cryosections are analyzed in a Siemens Elmiskop 102 electron microscope equipped with a Kevex energy dispersive system, and the elemental concentrations (in mMol/kg d.w.) of Na, Mg, P, S, Cl, K, and Ca are determined in subcellular compartments of muscle frozen in different functional states. The methodology of quantitation, i.e, determination of elemental net peak and continuum, correction of continuum, preparation of standards, and deconvolution of overlapping peaks are described. The minimum detectable elemental concentration using the reported methods is in the range of a few mMol/kg d.w. This also applies to Ca, which can be accumulated in heart muscle in readily detectable amounts in intracellularly located stores as well as structures connected with the cell membrane. The present report shows that cryotechniques and x-ray microanalysis can be successfully applied to heart physiology.
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  • 42
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    Journal of Electron Microscopy Technique 1 (1984) 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 43
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    Journal of Electron Microscopy Technique 1 (1984), S. 37-52 
    ISSN: 0741-0581
    Keywords: Electron energy loss spectroscopy ; Parallel detection ; Photodiode assays ; Fluorescent screens ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The present report paper deals with the use of a photodiode array for recording electron energy loss spectra in a transmission electron microscope. Important properties of the array are outlined, together with a description of the circuitry needed for interfacing the output to a multichannel analyser.In the direct-exposure mode, the device can easily detect a single (80 or 100 keV) electron, allowing inner-shell energy losses between 200 eV and 2000 eV to be recorded in about 10 seconds. By signal averaging a large number of readouts, a dynamic range of at least 105 is possible. Irradiation damage to the array can be controlled by cooling the array and by various anealing procedures. Sensitivity and DQE are lower, but the dynamic range is higher in the indirect mode, where a fluorescent screen is used to convert the electrons into visible photons, which are then imaged onto the diodes. The choice of screen material and of optical coupling to the array are discussed. Several spectral artifacts are described, together with spectrum-processing techniques designed to remove them.
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  • 44
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    Journal of Electron Microscopy Technique 1 (1984), S. 97-98 
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
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  • 45
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    Journal of Electron Microscopy Technique 1 (1984), S. 210-210 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 46
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    Journal of Electron Microscopy Technique 1 (1984), S. 341-348 
    ISSN: 0741-0581
    Keywords: Vascular casts ; Scanning electron microscopy ; Vascular anatomy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Corrosion casts provide three dimensional replicas that can be examined readily by scanning electron microscopy (SEM). They are prepared by filling vascular networks with polymerizing plastic and then digesting away the tissue. As based on our studies of ocular vessels, this report describes the vascular anatomy, as well as the artifacts, that are encountered during SEM studies of such preparations.
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  • 47
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    Journal of Electron Microscopy Technique 1 (1984), S. 317-329 
    ISSN: 0741-0581
    Keywords: Opioids ; Receptors ; Brain ; Radioautography ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Two met-enkephalin analogs (FK 33-824 and FW 34-569, Sandoz) were utilized for in vitro labeling of opioid binding sites in the rat central nervous system. Binding kinetics determined in 20-μm-thick frozen tissue sections of the striatum revealed that both pentapeptides bind to a single population of sites at 20°C with an apparent dissociation constant (KD) of approximately 1-2 nM and a maximum capacity (B max) of 65-170 fmoles/mg protein. Radioautographic data suggest that this population is the same for iodinated and tritiated forms of the FK compound and the iodinated FW analog. Fixation of labeled sections with high concentrations of glutaraldehyde allowed proportional retention of more than 50% of specifically bound 125I-FK molecules in all brain regions after histological processing for high-resolution radioautography. In contrast, glutaraldehyde fixation did not prevent the loss of bound 125I-FW molecules. These differences are attributed to the presence in FK, but not in FW molecules, of a free primary amino group considered essential for cross-link formation between aldehydes and proteins, and imply that a majority of FK-receptor complexes may be stabilized by glutaraldehyde. Consistent with this observation is the fact that the radioautographic distribution of specifically bound 125I-FK was unchanged after fixation and dehydration. In electron microscopic radioautographs prepared from prefixed, vibratome-cut striatal sections that were incubated with 125I-FK and fixed with glutaraldehyde, silver grains were found to be mostly associated with neuronal plasma membrane interfaces. The present methodological approach thus appears to be compatible with electron microscopic localization of opioid binding sites in the central nervous system and might be applicable to the localization of other types of binding sites using radioligand molecules that contain a free primary amino group.
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  • 48
    Publication Date: 2009-12-01
    Description: Vplyv rozdielnych porastov na úroveń hladín podzemných vûod v národnej prírodnej rezervácii Kláštorské LúkyMokrade sú citlivé ekosystémy charakteristické výskytom hladiny podzemnej vody v blízkosti terénu alebo aj na jeho povrchu. Významným mokradným systémom je aj národná prírodná rezervácia (NPR) Kláštorské lúky, ležiaca v povodí rieky Turiec. Zmena vo vlastníckych vzťahoch spôsobila aj zmenu ob-hospodarovania mokrade a vzácne travinno-bylinné porasty sú postupne nahrádzané spoločenstvami inváznymi, najmä tŕstím. Ďalším nepriaznivým javom, ku ktorému došlo v poslednom období v NPR, je pokles hladiny podzemnej vody (HPV). Táto práca je zameraná na zistenie možného vplyvu manažmentu na evapotranspiráciu a jej štruktúru, na zmeny chodov HPV a na zmeny obsahu vody vo vrchnej vrstve pôdy, spôsobené nahradením pôvodného porastu trávy inváznym porastom - tŕstím.
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  • 49
    Publication Date: 2009-12-01
    Description: Risks Due to Variability of K-Day Extreme Precipitation Totals and Other K-Day Extreme EventsSeveral alternative definitions of extreme events are proposed. As the first step a statistical analysis of daily precipitation measurement time series from the Hurbanovo SHMI Observatory and elaboration of potentially dangerous precipitation events is carried out. Then, combined characteristics based on daily temperature, daily air humidity and daily precipitation totals are computed. The drought index based on normalized deviations from long-term averages is defined. Alternatively, to define extreme events "Data envelopment analysis" (DEA) is employed withK-day periods of values of temperature, humidity and precipitation corresponding to decision making units. In this paper we have used the period ofK= 10 days for both methodologies for identification of extreme events. The results of all definitions of extreme events are compared.
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  • 50
    Publication Date: 2009-12-01
    Description: Hybrid Approach to Delineation of Homogeneous Regions for Regional Precipitation Frequency AnalysisRegional frequency analysis of heavy precipitation amounts based on the estimation of the parameters of a regional distribution function usingL-moments is adopted for the specific geographical-climatological settings of Slovakia. The paper focuses on the first step of the regionalL-moment algorithm (Hosking, Wallis, 1997), which is the delineation of homogeneous regions. Objective and process-based logical pooling techniques are used to form homogeneous pooling groups of rainfall gauging stations for regional frequency analysis ofk-day precipitation amounts (k= 1 to 5 days). Even though the delineation of homo-geneous regions by means of objective methods is generally accepted and recommended in the literature, it is concluded here that such a pooling of similar sites should not be carried out automatically in precipitation analysis. Instead, a combination of physical/geomorphological considerations and objective methods should be preferred.
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  • 51
    Publication Date: 2009-09-01
    Description: Numerical model of spherical particle saltation in a channel with a transversely tilted rough bedThis paper deals with the numerical simulation of spherical particle saltation in a channel with a rough transversely tilted bed. The numerical model presented is based on the 3D model of spherical particle saltation developed by the authors, which takes into account the translational and rotational particle motion. The stochastic method and the concept of a contact zone were used for the calculation of a particle trajectory and its dependence on the bed lateral slope, particle diameter, and shear velocity. The effect of the bed lateral slope results in a deviation of the particle trajectory from the downstream direction. Some examples of the calculation are presented. The trajectories of the saltating particles starting their movements from one point were calculated and it was shown that they are of random character and together create a bundle or fascicle of trajectories. It was found that the centrelines of the bundles can be approximated by the straight lines for low and moderate values of the bed transverse slope, i.e. slopes less than 20°. The angle of deviation of the centreline from the downstream direction increases when the bed lateral slope and/or the particle diameters increase. However, with increasing shear velocity, the deviation angle decreases. Due to the lateral bed slope the particles are sorted according to their size, and the criteria for sorting particles were defined. An example of the particle sorting was calculated and the separable and non-separable regions were determined.
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  • 52
    Publication Date: 2009-01-01
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  • 53
    Publication Date: 2009-09-01
    Description: On equivalent roughness of mobile bed at high shear stressThe friction conditions at the top of a mobile bed are discussed for flows in the upper-plane-bed regime, i.e. for the flows with values of the bed Shields parameter larger than approximately 0.6. A special attention is devoted to flows of the bed Shields parameter bigger than 2. Experimental data for flows at high bed shear are collected from literature and new data are added from own measurements of flows of a 1.36-mm sand slurry in the 100-mm pipe loop of the Institute of Hydrodynamics. The database represents flows of very different solids and covers friction conditions within a broad range of Shields parameters up to the maximum value of about 23. The paper analyses the data in order to evaluate a relationship among the equivalent roughness of the top of the bed and other relevant parameters. A semi-empirical formula is proposed that relates the equivalent roughness to the bed Shields parameter, the ratio of flow velocity and particle settling velocity, and the ratio of flow hydraulic radius and particle diameter. The formula is applicable primarily to flows of combined load (contact- and suspended loads together).
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  • 54
    Publication Date: 2009-12-01
    Description: Application of the Frier Distributed Model for Estimating the Impact of Land use Changes on the Water Balance in Selected Basins in SlovakiaIn this study, the FRIER rainfall-runoff model with distributed parameters was developed to assess changes in runoff and water balance due to changes in land use and climate. The water balance was calculated at 3 levels: on the surface and in unsaturated and saturated zones. Six basins from the central and eastern parts of Slovakia were selected on the basis of their similar size, but different topography, land use, soil texture and climate: the upper Hornád, the upper Hron, the Poprad, the Rimava, the Slaná and the Torysa River basins. Model parameters were estimated using data from the period from June 1998 to May 2000 in daily time steps. The differences and similarities of the hydrologic processes in individual basins were investigated during the calibration period. Several scenarios of changes in land use and two simple scenarios of changes in climate were developed to estimate the impact of these changes on water balance and runoff. The changes in the hydrological regime were compared and discussed.
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  • 55
    Publication Date: 2009-01-01
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  • 56
    Publication Date: 2009-01-01
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  • 57
    Publication Date: 2009-09-01
    Description: Runoff changes in areas differing in land-use in the blanice river basin - application of the deterministic modelThe aim of this article is to present partial results of more extensive research which is focused on using different methods for runoff computation in areas differing in land use. With the help of the deterministic lumped model HEC-HMS (Hydrologic Engineering Center - Hydrologic Modelling System) several simulations of r noff changes by different basin conditions were carried out. The Blanice River basin in the Šumava Mts. was chosen as an experimental catchment in its closure profile in Podedvory (gauge station, area 209.6 km2). For assessment of land cover changes impact on hydrological regime four scenarios were carried out - 10, 20, 50 and 100-year 1-day probability precipitation in combination with different initial conditions (soil saturation). These scenarios were applied to the stage of the land cover in the year 1992 and 2000 (based on the CORINE Landcover database). The method SCS CN (Soil Conservation Service Curve Number) was applied as the main model technique.
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  • 58
    Publication Date: 2009-03-01
    Description: Prediction of Water Quality in the Danube River Under extreme Hydrological and Temperature ConditionsOne of the requirements imposed by the Water Framework Directive (WFD, 2000/60/EC) is to analyze and predict how quality of surface waters will evolve in the future. In assessing the development of a stream's pollution one must consider all sources of pollution and understand how water quality evolves over time. Flow and water temperature regime of a stream or river are the main factors controlling the extent to which deterioration of a stream's water quality can propagate under constant input from pollution sources. In addition, there is ever increasing public concern about the state of the aquatic environment. Decision makers and scientists involved in water management call for studies proposing simulation models of water quality under extreme natural hydrologic and climatic scenarios. Also, human impact on water resources remain an issue for discussion, especially when it comes to sustainability of water resources with respect to water quality and ecosystem health. In the present study we investigate the long-term trends in water quality variables of the Danube River at Bratislava, Slovakia (Chl-a, Ca, EC, SO2-, Cl-, O2, BOD5, N-tot, PO4-P, NO3-N, NO2-N, etc.), for the period 1991-2005. Several SARIMA models were tested for the long-term prediction of selected pollutant concentrations under various flow and water temperature conditions. In order to create scenarios of selected water quality variables with prediction for 12 months ahead, three types of possible hydrologic and water temperature conditions were defined: i) average conditions - median flows and water temperature; ii) low flows and high water temperature; and iii) high flows and low water temperature. These conditions were derived for each month using daily observations of water temperature and daily discharge readings taken in the Danube at Bratislava over the period 1931-2005 in the form of percentiles (1th-percentile, median, 99th-percentile). Once having derived these extreme-case scenarios, we used selected Box-Jenkins models (with two regressors - discharge and water temperature) to simulate the extreme monthly water quality variables. The impact of natural and man-made changes in a stream's hydrology on water quality can be readily well simulated by means of autoregressive models.
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  • 59
    Publication Date: 2009-01-01
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    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 60
    Publication Date: 2009-06-01
    Description: The Effect of Cellular Organic Matter Produced by Cyanobacteria Microcystis Aeruginosa on Water PurificationThe aim of this paper is to investigate the influence of COM (Cellular Organic Matter) produced byMicrocystis aeruginosaon the process of water purification by destabilisation and subsequent aggregation of the impurity particles. The research was carried out with a raw water into which COM was added. The removal efficiency of the most significant components of COM, i.e. polysaccharides and proteins, was investigated. It was found that the removal efficiency of polysaccharides and proteins was dependent on the reaction conditions (pH, type of destabilisation reagent and its dosage). The removal efficiency of COM was relatively low. It was about 46% and 41% using ferric sulphate and aluminium sulphate aggregation, respectively. In comparison to the other organic components of COM, mainly polysaccharides, the proteins are removed with a higher efficiency. The GPC analyses of the residual COM showed that the proteins of higher molecular weight were aggregated with a higher efficiency.
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  • 61
    Publication Date: 2009-03-01
    Description: Malá Vodnosť Vybraných Vodných Tokov SlovenskaV predloženom článku hodnotíme hydrologické pozorovania na vybraných slovenských vodných tokoch za 75-ročné obdobie z hľadiska malej vodnosti. Hodnotíme charakteristiky: trvanie malej vodnosti a nedostatkový objem pre prahovú hodnotu prietokuQ90 (90% zabezpečenosti prietoku). Určili sme frekvenciu výskytu, zákon rozdelenia maximálnych nedostatkových objemov a maximálneho trvania malej vodnosti. Analyzovali sme charakteristiky pre najvýznamnejšie zaznamenané udalosti malej vodnosti - historické suchá v celom období pozorovania. Postup sme aplikovali na hydrologický rok a pre vybrané charakteristiky aj na letnú a zimnú sezónu.
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    Publication Date: 2009-01-01
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  • 63
    Publication Date: 2009-06-01
    Description: Analytical Solution for Transient Hydraulic Head, Flow Rate and Volumetric Exchange in an Aquifer Under Recharge ConditionThis paper presents closed form solution for unsteady flow equation corresponding to the transient hydraulic head, flow rate and volumetric exchange of a confined aquifer which is in contact with a constant piezometric head at one end and a stream whose water level is rising at a constant rate at the other end. The aquifer is also subjected to receive constant inflow due to rain infiltration. The unsteady groundwater flow equation is solved using Laplace transform to get analytical expressions for the transient hydraulic head and flow rate at the left and right interfaces and the net volumetric exchange of water at the aquifer-stream interface. The analytical results presented here show the effect of recharge due to rain infiltration on the net volumetric exchange and reveal the conditions for which net inflow in the aquifer could be positive, negative or zero. The results obtained have the capability to determine transient hydraulic head for two extreme scenarios: (i) very slow rise and (ii) very fast rise in the stream water. Analytical result show that the net volumetric exchange could be positive, zero or negative depending on the surface infiltration and stream water rise rate.
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 64
    Publication Date: 2009-01-01
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 65
    Publication Date: 2009-01-01
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 66
    Publication Date: 2009-09-01
    Description: Estimating high levels exceedance probabilities by point process approach with applications to northern Moravia precipitation and discharges seriesThe paper byJaruškováandHanek(2006) advocated application of the peaks over threshold method (POT method) for estimating the probability that a precipitation or discharges series exceeds a chosen high level. If daily precipitation amounts or average discharges are obtained at several stations one might be interested in estimating the probability that in the same time all variables of interest, e.g. precipitation amounts measured at several stations, exceed some chosen high levels. The paper explains how the method based on the point process approach may be used to get good estimates of such probabilities. Moreover, it presents some useful parametric models that were successfully applied by the author to some precipitation and discharges series of northern Moravia.
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 67
    Publication Date: 2009-01-01
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 68
    Publication Date: 2009-12-01
    Description: Transforming Hydrographs in the Hillslope SubsurfaceTo reveal and evaluate the mechanism of transforming rainfall into runoff in the region, where the subsurface flow plays a dominant role in the runoff formation, a continuous hydrological and climatic data monitoring has been set-up in the experimental catchment Uhlířská (the Jizera Mountains, CR). The soil profile (Dystric Cambisol), formed on the weathered granite bedrock, is shallow and highly heterogeneous. Beside a standard catchment data observation a hillslope transect was instrumented to control the flow dynamics in the soil profile. From three soil horizons, the subsurface outflow is recorded in the subsurface trench. Adjacent to the trench the soil water suction is scanned by triplets of automatic tensiometers. Within the soil profile the unsaturated regime prevails, nevertheless the soil keeps almost saturated. Nearly simultaneous reaction of suction on a rainfall in all soil horizons implies a rapid vertical flow. Local preferential flow paths are conducting infiltrating water at significantly variable rates when saturation is reached. Groundwater table, soil moisture and subsurface runoff measured at the hillslope transect and the total outflow from the catchment, are correlated. The outflow from the catchment is dominantly controlled by soil moisture however the mechanism of its generation is not yet fully understood.
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 69
    Publication Date: 2009-09-01
    Description: Identification of compound channel flow parametersOptimization methods are used to estimate parameters required for routing floods through open compound channels. Besides initial and boundary flow conditions, data required especially include, cross-sectional area (A) of flow and conveyance (K) as functions of flow depth (y) for a representative cross-section of the study reach. Thus, instead of optimizing upon channel's geometric and hydraulic parameters, optimization is performed upon non-physical parameters in assumedA(y) andK(y) relationships. The optimization method selected for this application is the Nelder and Mead Simplex Algorithm. The objective function is expressed in terms of the relative differences between observed and simulated stages and discharges, which are evaluated based on the complete numerical solution of St Venant equations. This approach to formulating the optimization problem was applied to unsteady flow data sets for an experimental reach of the River Main in Northern Ireland. Based on statistical analysis, simulated and observed stages were found to be in good agreement.
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 70
    Publication Date: 2009-03-01
    Description: Crop Water Supply and its Relation to Yield of Spring Wheat in the South of Russian PlainThe proposed method to estimate water supply of spring wheat crop is based on the ratio of the water amount extracted by plants under actual conditions of growth (transpiration) to cover needs for maximum (potential) yield (potential transpiration). Estimates of spatial, inter- and intra-annual water supply variability of the spring wheat crop in basic agricultural zones are given. Dependence of the spring wheat yield on water supply is presented.
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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  • 71
    Publication Date: 2009-01-01
    Electronic ISSN: 0042-790X
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Natural Sciences in General
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