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  • Books  (15)
  • Articles  (1,099,745)
  • 2010-2014  (747,400)
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  • Books  (15)
  • Articles  (1,099,745)
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  • 1
    Unknown
    Urbanization, Biodiversity and Ecosystem Services: Challenges and Opportunities : A Global Assessment (2013)
    Dordrecht : Springer
    Details
    Keywords: Life sciences ; Science ; Urban planning ; City planning ; Urban ecology (Biology) ; System theory ; International environmental law ; Sustainable development ; Life Sciences ; Urban Ecology ; Urbanism ; Sustainable Development ; Complex Systems ; Science, general ; International Environmental Law
    Description / Table of Contents: 1. A global outlook on urbanization --- 2. History of urbanization and the missing ecology --- 3. Urbanization and global trends in biodiversity and ecosystem services --- 4. Regional assessment of Asia.- 5. Sub-regional assessment of China: Urbanization in biodiversity hotspots --- 6. Sub-regional assessment of India --- 7. Local assessment of Bangalore.- 8. Local assessment of Tokyo --- 9. Local assessment of Shanghai --- 10. Patterns and trends in urban biodiversity and landscape design --- 11. Urban ecosystem services.- 12. Shrinking cities, biodiversity and ecosystem services --- 13. Regional assessments of Europe --- 14. Regional assessment of North America --- 15. Regional assessment of Oceania --- 16. Local assessment of Istanbul: Biodiversity and ecosystem services --- 17. Local assessment of Stockholm --- 18. Local assessment of Chicago --- 19. Local assessment of New York City.-20. Local assessment of Melbourne --- 21. A synthesis of global urbanization projections --- 22. Urbanization forecasts, effects on land use, biodiversity, and ecosystem services --- 23. Regional assessment of Africa --- 24. Local assessment of Cape Town.- 25. Climate change and urban biodiversity vulnerability --- 26. Feeding cities --- 27. Urban governance of biodiversity and ecosystem services --- 28. Regional assessment of Latin America --- 29. Local assessment of Rio de Janeiro.- 30. Urban landscapes as learning arenas for biodiversity and ecosystem services management --- 31. Restoration ecology in an urbanizing world --- 32. Indicators for management of urban biodiversity and ecosystem services --- 33. Stewardship of the Biosphere in the Urban Era
    Pages: Online-Ressource (XXVIII, 755 pages) , 150 illustrations, 23 illustrations in color
    ISBN: 9789400770881
    URL: https://link.springer.com/openurl?genre=book&isbn=978-94-007-7088-1
    Language: English
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  • 2
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    Echinoderm Research 2010 : 7th European Conference on Echinoderms, Göttingen, October 2 - 9, 2010 ; abstract volume and field guide to excursions (2010)
    Göttingen : Universitätsverlag Göttingen
    Details
    Description / Table of Contents: Echinoderms are a vast group of spiny-skinned animals including starfish, brittle-stars, sea urchins, sand dollars, feather stars, sea lilies and sea cucumbers. These relatives of chordates and hemichordates have inhabited the world’s oceans for more than 500 million years. Modern members of the Echinodermata are, with over 7 000 species, an integral part of marine communities from the intertidal to the deep sea. Echinoderms play a major ecological role in marine habitats and are of economic importance in fisheries, aquaculture and biomedicine. The present volume contains the abstracts of lectures and posters presented during the 7th European Conference on Echinoderms (ECE) as well as excursion guides. This year’s conference was held at the northern campus of the Georg-August University in Göttingen, Germany, from October 2-9, 2010. More than 100 biologists, palaeontologists and other scientists from 25 countries participated.
    Pages: Online-Ressource (145 Seiten)
    ISBN: 9783941875685
    URL: http://www.univerlag.uni-goettingen.de/content/list.php?details=isbn-978-3-941875-68-5
    Language: English
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  • 3
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    Sexual Reproduction in Animals and Plants (2014)
    Tokyo : Springer
    Details
    Keywords: Life sciences ; Cell biology ; Developmental biology ; Plant science ; Botany ; Life Sciences ; Developmental Biology ; Plant Sciences ; Cell Biology
    Description / Table of Contents: Part 1 Sperm Attraction, Activation and Acrosome Reaction --- 1 Sperm Chemotaxis: The First Authentication Events Between Conspecific Gametes Prior to Fertilization (Manabu Yoshida) --- 2 Respiratory CO2 Mediates Sperm Chemotaxis in Squids (Noritaka Hirohashi) --- 3 Specific Mechanism of Sperm Storage in Avian Oviducts (Tomohiro Sasanami) --- 4 Allurin: Exploring the Activity of a Frog Sperm Chemoattractant in Mammals (Douglas E. Chandler) --- 5 Structure, Function and Phylogenetic Consideration of Calaxin (Kazuo Inaba) --- 6 Cl- Channels and Transporters in Sperm Physiology (Alberto Darszon) --- 7 Equatorin-related Subcellular and Molecular Events During Sperm Priming for Fertilization in Mice (Kiyotaka Toshimori) --- 8 Acrosome Reaction-mediated Motility Initiation that is Critical for the Internal Fertilization of Urodele Amphibians (Akihiko Watanabe) --- 9 Analysis of the Mechanism that Brings Protein Disulfide Isomerase-P5 to Inhibit Oxidative Refolding of Lysozyme (Miho Miyakawa) --- Part 2 Gametogenesis, Gamete Recognition, Activation, and Evolution --- 10 Effect of Relaxin-like Gonad-Stimulating Substance (GSS) on Gamete Shedding and 1-Methyladenine Production in Starfish Ovaries (Masatoshi Mita) --- 11 Incapacity of 1-Methyladenine Production to Relaxin-like Gonad-Stimulating Substance (GSS) in Ca2+-free Seawater-treated Starfish Ovarian Follicle Cells (Masatoshi Mita) --- 12 Novel Isoform of Vitellogenin Expressed in Eggs is a Binding Partner of the Sperm Proteases, HrProacrosin and HrSermosin, in the Ascidian Halocynthia roretzi (Hitoshi Sawada) --- 13 Actin Cytoskeleton and Fertilization in Starfish Eggs (Luigia Santella) --- 14 Focused Proteomics on Egg Membrane Microdomains to Elucidate the Cellular and Molecular Mechanisms of Fertilization in the African Clawed Frog Xenopus laevis (Ken-ichi Sato) --- 15 Egg Activation in Polyspermy: Its Molecular Mechanisms and Evolution in Vertebrates (Yasuhiro Iwao ) --- 16 ATP Imaging in Xenopus laevis Oocyte (Takashi Ijiri) --- 17 Mitochondrial Activation and Nitric Oxide (NO) Release at Fertilization in Echinoderm Eggs (Tatsuma Mohri) --- 18 Functional Roles of Spe Genes in the Male Germline During Reproduction of Caenorhabditis elegans (Hitoshi Nishimura) --- 19 Origin of Female/Male Gender as Deduced by the Mating Type Loci of the Colonial Volvocalean Greens (Hisayoshi Nozaki) --- Part 3 Allorecognition in Male–Female Interaction --- 20 Allorecognition and Lysin Systems During Ascidian Fertilization (Hitoshi Sawada) --- 21 Self-incompatibility in the Brassicaceae (Megumi Iwano) --- 22 Signalling Events in Pollen Acceptance or Rejection in the Arabidopsis Species (Daphne R. Goring) --- 23 Papaver rhoeas S-Determinants and the Signaling Networks they Trigger (Vernonica E. Franklin-Tong ) --- 24 S-RNase-based Self-incompatibility in Petunia: A Complex Non-self Recognition System Between Pollen and Pistil (Teh-hui Kao) --- 25 Self-incompatibility System of Ipomoea trifida, a Wild-type Sweet Potato (Tohru Tsuchiya) --- Part 4 Male–Female Interaction and Gamete Fusion --- 26 Profiling the GCS1-based Gamete Fusion Mechanism (Toshiyuki Mori) --- 27 Fertilization Mechanisms of the Rodent Malarial Parasite Plasmodium berghei (Makoto Hirai) --- 28 Sexual Reproduction of a Unicellular Charophycean Alga, Closterium peracerosum-strogosum-littorale Complex (Hiroyuki Sekimoto) --- 29 Fertilization of Brown Algae: Flagellar Function in Phototaxis and Chemotaxis (Taizo Motomura ) --- 30 Gene and Protein Expression Profiles in Rice Gametes and Zygotes: A Cue for Understanding the Mechanisms of Gametic and Early Zygotic Development in Angiosperms (Takashi Okamoto) --- 31 Role of CD9 in Sperm-Egg Fusion and Virus-induced Cell Fusion in Mammals (Kenji Miyado) --- 32 The Mechanism of Sperm-Egg Fusion in Mouse and the Involvement of IZUMO1 (Naokazu Inoue) --- 33 A ZP2 Cleavage Model of Gamete Recognition and the Post-fertilization Block to Polyspermy (Jurrien Dean) --- 34 Involvement of Carbohydrate Residues of the Zona Pellucida in In Vitro Sperm Recognition in Pigs and Cattle (Naoto Yonezawa) --- Part 5 Organella, Proteolysis, and New Techniques --- 35 The Role of Peroxisomes in Plant Reproductive Processes (Shoji Mano) --- 36 Regulation of Vacuole-mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants (Tomoko Koyano) --- 37 Sperm Proteasomes as a Putative Egg Coat Lysin in Mammals (Peter Sutovsky) --- 38 Germline Transformation in the Ascidian Ciona intestinalis (Yasunori Sasakura) --- BM Index.  
    Pages: Online-Ressource (XII, 480 pages) , 127 illustrations, 102 illustrations in color
    ISBN: 9784431545897
    URL: https://link.springer.com/openurl?genre=book&isbn=978-4-431-54589-7
    Language: English
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  • 4
    Unknown
    Interdisciplinary Analysis and Modeling of Carbon-Optimized Land Management Strategies for Southern Amazonia : Carbiocial Status Conference in Cuiabá, October 7-8, 2013 (2014)
    Göttingen : Universitätsverlag Göttingen
    Details
    Keywords: land management ; Amazonia ; global change
    Description / Table of Contents: The Carbiocial Project investigates viable carbon-optimized land management strategies for maintaining tropical ecosystem services under land use change and changing climate conditions in Southern Amazonia – a hotspot of global change. The project aims at understanding the vital natural processes and socio-economic driving forces in the region and develops strategies to enhance and protect carbon stocks in the recently deforested agroscapes of Central/Northern Mato Grosso and South Pará. That is why Carbiocial analyzes and models soil, water and climate as well as agro-economics, social and political transformations. Based on detailed storylines, the project aims at identifying possible entry-points for a necessary change in local and regional production patterns, considering local livelihoods as well as the present national and global economic, legal and political situation. This book gives an overview of the first results of the multi-disciplinary Carbiocial Project by publishing the main presentations, held on the Carbiocial Status Conference, on October 7-8, 2013, in Cuiabá. In sixteen chapters the authors elucidate the project‘s current state of knowledge, illustrating adapted methods for regional modeling and promising strategies for the Amazon development. | Contents --- Stefan Hohnwald & Gerhard Gerold: Carbon-Optimized Land Management Research for the Southern Amazon-Geographical and Organizational Settings of the Carbiocial-Carbioma Project Consortium --- Philip M. Fearnside, Aurora M. Yanai & Claudia S. M. N. Vitel: Modeling Baselines for REDD Projects in Amazonia: Is the Carbon Real? --- Jens Boy, Charlotte Schumann, Simone Strey, Robert Strey, Georg Guggenberger & Regine Schönenberg: Digging Deeper – Biographic Interviews as a Promising Tool for the Joint Dissemination of Natural- and Social Science Results in REDD Contexts --- Carlos E. P. Cerri, Thalita F. Abbruzzini, Carolina B. Brandani, Mariana R. Durigan & Denise Signor: Soil Carbon Stocks and Greenhouse Gas Emissions from Agrosystems in Brazil --- Karl M. Wantzen, Malte Unger, Eduardo G. Couto, Ricardo S. S. Amorim, Karina P. Peña & Ulrich Irmler: Carbon Farming: Enriching Tropical Farm Soils with Organic Matter --- Alphonce C. Guzha, Ricardo S. S. Amorim, Rodolfo Nóbrega, Gabriele Lamparter, Kristof Kovacs, Norma Bertão & Gerhard Gerold: Impacts of Land Cover and Climate Change on Hydrology and Hydrochemistry in Selected Catchments in Southern Amazonia: Preliminary Analysis and Results --- Marcus Schindewolf, Daniela Schönke, Ricardo S. S. Amorim & Jürgen Schmidt: Effects of Contour Banks and No-Till Measures on Run-Off and Sediment Yield in Campo Verde Region, Mato Grosso --- Alessandra R. Gomes, César G. Diniz & Cláudio A. Almeida: Amazon Regional Center (INPE/CRA) Actions for Brazilian Amazon Forest: TerraClass and Capacity Building Projects --- Patrick Hostert, Tobia Lakes, Hannes Müller, Florian Gollnow & Letícia B. V. Hissa: Land-Use Monitoring and Change Detection --- Jürgen Böhner, Helge Dietrich, Klaus Fraedrich, Tobias Kawohl, Markus Kilian, Valerio Lucarini & Frank Lunkeit: Development and Implementation of a Hierarchical Model Chain for Modelling Regional Climate Variability and Climate Change Over Southern Amazonia --- Claas Nendel, Hermann Jungkunst & Adriano M. R. Figueiredo: Intercol and Steps Towards a Simplified DSS --- Neli A. de Mello-Théry & Paulo R. Cunha: Environmental Policies and Forest Code: Changes and Repercussions on the Agriculture in Mato Grosso --- Regine Schönenberg, Korbinian Hartberger & Charlotte Schumann: Challenges and Chances of Social Transformation for GHG-Optimized Land- and Natural Resource Management Strategies: Stakeholder-Dialogues as Prerequisite for the Elaboration of Applicable Results --- José H. Benatti & Luly R. da Cunha Fischer: Land Use Regulations in the State of Pará: An Introductory Approach of Its Guidelines --- Martin Coy, Michael Klingler, Matthias Siebold & Thomas Berger: Socio-Economic Regional Change and Agro-Economic Development Along the BR-163 --- Edna Castro: Deforestation Along the BR-163: Socio-Environmental Conflicts and Ignored Governmental Politics
    Pages: Online-Ressource (174 Seiten)
    ISBN: 9783863951382
    URL: http://resolver.sub.uni-goettingen.de/purl?isbn-978-3-86395-138-2
    Language: English
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  • 5
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    Ecophysiology of Root Systems-Environment Interactions (2014)
    Lausanne : Frontiers
    Details
    Keywords: botany ; earthworms ; ectomycorrhiza ; drought ; heavy metal ; Infertile soils ; neighbour perception ; plasticity ; root systems ; root traits ; rhizobacteria ; rhizosphere ; deep roots
    Description / Table of Contents: Plant sciences research focuses predominantly on aboveground parameters. There is a scarcity of detailed information regarding the ecophysiology of root systems and the way root system functioning is affected by both internal and external factors. Furthermore, global climate change is expected to increase the intensity of climate extremes, such as severe drought, heat waves and periods of heavy rainfall; in addition other stresses such as salinization of soils are increasing world-wide. Recently an increasing awareness has developed that understanding plant traits will play a major role in breeding of future crop plants. For example, there is increasing evidence that the traits of root systems are defined by the properties of individual roots. However, further knowledge on the functional importance of root segments and the molecular/physiological mechanisms underlying root system functioning and persistence is needed, and would specifically allow modifying (crop) root system functionality and efficiency in the future. Another major gap in knowledge is localized at the root-soil interface and in regard to the potential adaptive plasticity of root-rhizosphere interactions under abiotic stress and/or competition. It is currently unknown whether adaptations in microbe communities occur, for example due to modified exudation rates, and what are the subsequent influences on nutrient mobilization and uptake. Furthermore, uncovering the mechanisms by which roots perceive neighboring roots may not only contribute to our understanding of plant developmental strategies, but also has important implications on the study of competitive interactions in natural communities, and in optimizing plant performance and resource use in agricultural and silvicultural systems. In this research topic, we aim to provide an on-line, open-access snapshot of the current state of the art of the field of root ecology and physiology, with special foci on the translation of root structure to function, and how root systems are influenced by interplay with internal and external factors such as abiotic stress, microbes and plant-plant interaction. We warmly welcome original research papers, but reviews of specific topics, articles formulating opinions or describing cutting-edge methods are also gladly accepted.
    Pages: Online-Ressource (316 Seiten)
    ISBN: 9782889192854
    URL: https://doi.org/10.3389/978-2-88919-285-4
    Language: English
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  • 6
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    The Microbial Regulation of Global Biogeochemical Cycles (2014)
    Lausanne : Frontiers
    Details
    Keywords: microbial ecology ; biogeochemistry ; stoichiometry ; Climate Change ; soil microbiology ; elemental fluxes ; Respiration ; aquatic microbiology ; microbiology
    Description / Table of Contents: Global biogeochemical cycles of carbon and nutrients are increasingly affected by human activities. So far, modeling has been central for our understanding of how this will affect ecosystem functioning and the biogeochemical cycling of carbon and nutrients. These models have been forced to adopt a reductive approach built on the flow of carbon and nutrients between pools that are difficult or even impossible to verify with empirical evidence. Furthermore, while some of these models include the response in physiology, ecology and biogeography of primary producers to environmental change, the microbial part of the ecosystem is generally poorly represented or lacking altogether. The principal pool of carbon and nutrients in soil is the organic matter. The turnover of this reservoir is governed by microorganisms that act as catalytic converters of environmental conditions into biogeochemical cycling of carbon and nutrients. The dependency of this conversion activity on individual environmental conditions such as pH, moisture and temperature has been frequently studied. On the contrary, only rarely have the microorganisms involved in carrying out the processes been identified, and one of the biggest challenges for advancing our understanding of biogeochemical processes is to identify the microorganisms carrying out a specific set of metabolic processes and how they partition their carbon and nutrient use. We also need to identify the factors governing these activities and if they result in feedback mechanisms that alter the growth, activity and interaction between primary producers and microorganisms. By determining how different groups of microorganisms respond to individual environmental conditions by allocating carbon and nutrients to production of biomass, CO2 and other products, a mechanistic as well as quantitative understanding of formation and decomposition of organic matter, and the production and consumption of greenhouse gases, can be achieved. In this Research Topic, supported by the Swedish research councils' programme "Biodiversity and Ecosystem Services in a Changing Landscape" (BECC), we intend to promote this alternative framework to address how cycling of carbon and nutrients will be altered in a changing environment from the first-principle mechanisms that drive them – namely the ecology, physiology and biogeography of microorganisms – and on up to emerging global biogeochemical patterns. This novel and unconventional approach has the potential to generate fresh insights that can open up new horizons and stimulate rapid conceptual development in our basic understanding of the regulating factors for global biogeochemical cycles. The vision for the research topic is to facilitate such progress by bringing together leading scientists as proponents of several disciplines. By bridging Microbial Ecology and Biogeochemistry, connecting microbial activities at the micro-scale to carbon fluxes at the ecosystem-scale, and linking above- and belowground ecosystem functioning, we can leap forward from the current understanding of the global biogeochemical cycles.
    Pages: Online-Ressource (242 Seiten)
    ISBN: 9782889192977
    URL: https://doi.org/10.3389/978-2-88919-297-7
    Language: English
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  • 7
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    Global Change and Human Vulnerability to Vector-borne Diseases (2013)
    Lausanne : Frontiers
    Details
    Keywords: vector-borne diseases ; species composition ; mosquitos ; highland malaria ; virus ; larvicidal agent ; global change ; climate ; emerging and re-emerging disease
    Description / Table of Contents: It is well known that several climatic, environmental and socio-demographic changes that have occurred in the last years are some of the most important causes for the emergence/resurgence of vector-borne diseases worldwide. Global change can be defined as the impact of human activity on the fundamental mechanisms of biosphere functioning. Therefore, global change includes not only climate change, but also habitat transformation, water cycle modification, biodiversity loss, synanthropic incursion of alien species into new territories, or introduction of new chemicals in nature. On this respect, some of the effects of global change on vector-borne diseases can be currently evaluated. Globalization has enabled the movement of parasites, viruses and vectors among different countries, or even at intercontinental level. On this regard, it is important to note that the increase of imported malaria cases in different Southern European countries has led to the re-appearance of autochthonous cases of disease transmission. Moreover, the used tire trade, together with global warming, have facilitated the introduction, spread and establishment of potential Dengue tropical vectors, such as Aedes aegypti or Aedes albopictus in temperate areas. Consequently, recently the first Dengue indigenous cases in the last decades have been reported in different Southern areas of North America and Europe. Furthermore, habitat modification, mainly deforestation and transformation of aquatic environments, together with the changes in thermal and rainfall patterns, are two of the key factors to explain the increasing incidence of Leishmaniasis and several tick-borne diseases. The aim of this Research Topic is to cover all related fields with the binomial vector-borne diseases / global change, including basic and applied research, approaches to control measures, explanations of new theories, opinion articles, reviews, etc. To discuss these issues, a holistic and integrative point of view is necessary, which only would be achieved by the close and active participation of specialists on entomology, parasitology, virology and epidemiology. Our objective is to use a systems approach to the problem of global change and vector-borne diseases. To achieve this ambitious goal and to comply with a demand of first-rate scientific and medical interest, we are very keen on asking for the participation of multiple contributors.
    Pages: Online-Ressource (183 Seiten)
    ISBN: 9782889191567
    URL: https://doi.org/10.3389/978-2-88919-156-7
    Language: English
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  • 8
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    Sexual Reproduction in Animals and Plants (2014)
    Tokyo : Springer
    Details
    Keywords: Life sciences ; Cell biology ; Developmental biology ; Plant science ; Botany ; Life Sciences ; Developmental Biology ; Plant Sciences ; Cell Biology
    Description / Table of Contents: Part 1 Sperm Attraction, Activation and Acrosome Reaction --- 1 Sperm Chemotaxis: The First Authentication Events Between Conspecific Gametes Prior to Fertilization (Manabu Yoshida) --- 2 Respiratory CO2 Mediates Sperm Chemotaxis in Squids (Noritaka Hirohashi) --- 3 Specific Mechanism of Sperm Storage in Avian Oviducts (Tomohiro Sasanami) --- 4 Allurin: Exploring the Activity of a Frog Sperm Chemoattractant in Mammals (Douglas E. Chandler) --- 5 Structure, Function and Phylogenetic Consideration of Calaxin (Kazuo Inaba) --- 6 Cl- Channels and Transporters in Sperm Physiology (Alberto Darszon) --- 7 Equatorin-related Subcellular and Molecular Events During Sperm Priming for Fertilization in Mice (Kiyotaka Toshimori) --- 8 Acrosome Reaction-mediated Motility Initiation that is Critical for the Internal Fertilization of Urodele Amphibians (Akihiko Watanabe) --- 9 Analysis of the Mechanism that Brings Protein Disulfide Isomerase-P5 to Inhibit Oxidative Refolding of Lysozyme (Miho Miyakawa) --- Part 2 Gametogenesis, Gamete Recognition, Activation, and Evolution --- 10 Effect of Relaxin-like Gonad-Stimulating Substance (GSS) on Gamete Shedding and 1-Methyladenine Production in Starfish Ovaries (Masatoshi Mita) --- 11 Incapacity of 1-Methyladenine Production to Relaxin-like Gonad-Stimulating Substance (GSS) in Ca2+-free Seawater-treated Starfish Ovarian Follicle Cells (Masatoshi Mita) --- 12 Novel Isoform of Vitellogenin Expressed in Eggs is a Binding Partner of the Sperm Proteases, HrProacrosin and HrSermosin, in the Ascidian Halocynthia roretzi (Hitoshi Sawada) --- 13 Actin Cytoskeleton and Fertilization in Starfish Eggs (Luigia Santella) --- 14 Focused Proteomics on Egg Membrane Microdomains to Elucidate the Cellular and Molecular Mechanisms of Fertilization in the African Clawed Frog Xenopus laevis (Ken-ichi Sato) --- 15 Egg Activation in Polyspermy: Its Molecular Mechanisms and Evolution in Vertebrates (Yasuhiro Iwao ) --- 16 ATP Imaging in Xenopus laevis Oocyte (Takashi Ijiri) --- 17 Mitochondrial Activation and Nitric Oxide (NO) Release at Fertilization in Echinoderm Eggs (Tatsuma Mohri) --- 18 Functional Roles of Spe Genes in the Male Germline During Reproduction of Caenorhabditis elegans (Hitoshi Nishimura) --- 19 Origin of Female/Male Gender as Deduced by the Mating Type Loci of the Colonial Volvocalean Greens (Hisayoshi Nozaki) --- Part 3 Allorecognition in Male–Female Interaction --- 20 Allorecognition and Lysin Systems During Ascidian Fertilization (Hitoshi Sawada) --- 21 Self-incompatibility in the Brassicaceae (Megumi Iwano) --- 22 Signalling Events in Pollen Acceptance or Rejection in the Arabidopsis Species (Daphne R. Goring) --- 23 Papaver rhoeas S-Determinants and the Signaling Networks they Trigger (Vernonica E. Franklin-Tong ) --- 24 S-RNase-based Self-incompatibility in Petunia: A Complex Non-self Recognition System Between Pollen and Pistil (Teh-hui Kao) --- 25 Self-incompatibility System of Ipomoea trifida, a Wild-type Sweet Potato (Tohru Tsuchiya) --- Part 4 Male–Female Interaction and Gamete Fusion --- 26 Profiling the GCS1-based Gamete Fusion Mechanism (Toshiyuki Mori) --- 27 Fertilization Mechanisms of the Rodent Malarial Parasite Plasmodium berghei (Makoto Hirai) --- 28 Sexual Reproduction of a Unicellular Charophycean Alga, Closterium peracerosum-strogosum-littorale Complex (Hiroyuki Sekimoto) --- 29 Fertilization of Brown Algae: Flagellar Function in Phototaxis and Chemotaxis (Taizo Motomura ) --- 30 Gene and Protein Expression Profiles in Rice Gametes and Zygotes: A Cue for Understanding the Mechanisms of Gametic and Early Zygotic Development in Angiosperms (Takashi Okamoto) --- 31 Role of CD9 in Sperm-Egg Fusion and Virus-induced Cell Fusion in Mammals (Kenji Miyado) --- 32 The Mechanism of Sperm-Egg Fusion in Mouse and the Involvement of IZUMO1 (Naokazu Inoue) --- 33 A ZP2 Cleavage Model of Gamete Recognition and the Post-fertilization Block to Polyspermy (Jurrien Dean) --- 34 Involvement of Carbohydrate Residues of the Zona Pellucida in In Vitro Sperm Recognition in Pigs and Cattle (Naoto Yonezawa) --- Part 5 Organella, Proteolysis, and New Techniques --- 35 The Role of Peroxisomes in Plant Reproductive Processes (Shoji Mano) --- 36 Regulation of Vacuole-mediated Programmed Cell Death During Innate Immunity and Reproductive Development in Plants (Tomoko Koyano) --- 37 Sperm Proteasomes as a Putative Egg Coat Lysin in Mammals (Peter Sutovsky) --- 38 Germline Transformation in the Ascidian Ciona intestinalis (Yasunori Sasakura) --- BM Index.  
    Pages: Online-Ressource (XII, 480 pages) , 127 illustrations, 102 illustrations in color
    ISBN: 9784431545897
    URL: https://link.springer.com/openurl?genre=book&isbn=978-4-431-54589-7
    Language: English
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  • 9
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    Urbanization, Biodiversity and Ecosystem Services: Challenges and Opportunities : A Global Assessment (2013)
    Dordrecht : Springer
    Details
    Keywords: Life sciences ; Science ; Urban planning ; City planning ; Urban ecology (Biology) ; System theory ; International environmental law ; Sustainable development ; Life Sciences ; Urban Ecology ; Urbanism ; Sustainable Development ; Complex Systems ; Science, general ; International Environmental Law
    Description / Table of Contents: 1. A global outlook on urbanization --- 2. History of urbanization and the missing ecology --- 3. Urbanization and global trends in biodiversity and ecosystem services --- 4. Regional assessment of Asia.- 5. Sub-regional assessment of China: Urbanization in biodiversity hotspots --- 6. Sub-regional assessment of India --- 7. Local assessment of Bangalore.- 8. Local assessment of Tokyo --- 9. Local assessment of Shanghai --- 10. Patterns and trends in urban biodiversity and landscape design --- 11. Urban ecosystem services.- 12. Shrinking cities, biodiversity and ecosystem services --- 13. Regional assessments of Europe --- 14. Regional assessment of North America --- 15. Regional assessment of Oceania --- 16. Local assessment of Istanbul: Biodiversity and ecosystem services --- 17. Local assessment of Stockholm --- 18. Local assessment of Chicago --- 19. Local assessment of New York City.-20. Local assessment of Melbourne --- 21. A synthesis of global urbanization projections --- 22. Urbanization forecasts, effects on land use, biodiversity, and ecosystem services --- 23. Regional assessment of Africa --- 24. Local assessment of Cape Town.- 25. Climate change and urban biodiversity vulnerability --- 26. Feeding cities --- 27. Urban governance of biodiversity and ecosystem services --- 28. Regional assessment of Latin America --- 29. Local assessment of Rio de Janeiro.- 30. Urban landscapes as learning arenas for biodiversity and ecosystem services management --- 31. Restoration ecology in an urbanizing world --- 32. Indicators for management of urban biodiversity and ecosystem services --- 33. Stewardship of the Biosphere in the Urban Era
    Pages: Online-Ressource (XXVIII, 755 pages) , 150 illustrations, 23 illustrations in color
    ISBN: 9789400770881
    URL: https://link.springer.com/openurl?genre=book&isbn=978-94-007-7088-1
    Language: English
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  • 10
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    Self-Reported Population Health: An International Perspective based on EQ-5D (2014)
    Dordrecht : Springer
    Details
    Keywords: Medicine ; Public health ; Medical research ; Quality of life ; Biomedicine ; Biomedicine general ; Public Health ; Quality of Life Research
    Description / Table of Contents: Preface.- Data and Methods.- Population Norms for the EQ-5D --- Cross-Country Analysis of EQ-5D Data --- Socio-demographic Indicators based on EQ-5D --- Annex 1 --- Annex 2
    Pages: Online-Ressource (XV, 196 pages) , 14 illustrations, 9 illustrations in color
    ISBN: 9789400775961
    URL: https://link.springer.com/openurl?genre=book&isbn=978-94-007-7596-1
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  • 11
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    Self-Reported Population Health: An International Perspective based on EQ-5D (2014)
    Dordrecht : Springer
    Details
    Keywords: Medicine ; Public health ; Medical research ; Quality of life ; Biomedicine ; Biomedicine general ; Public Health ; Quality of Life Research
    Description / Table of Contents: Preface.- Data and Methods.- Population Norms for the EQ-5D --- Cross-Country Analysis of EQ-5D Data --- Socio-demographic Indicators based on EQ-5D --- Annex 1 --- Annex 2
    Pages: Online-Ressource (XV, 196 pages) , 14 illustrations, 9 illustrations in color
    ISBN: 9789400775961
    URL: https://link.springer.com/openurl?genre=book&isbn=978-94-007-7596-1
    Language: English
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    Application of Geographic Information Systems (2012)
    Rijeka : InTech
    Details
    Keywords: geographics ; GIS ; Geographic Information Systems
    Description / Table of Contents: The importance of Geographic Information Systems (GIS) can hardly be overemphasized in today’s academic and professional arena. More professionals and academics have been using GIS than ever – urban & regional planners, civil engineers, geographers, spatial economists, sociologists, environmental scientists, criminal justice professionals, political scientists, and alike. As such, it is extremely important to understand the theories and applications of GIS in our teaching, professional work, and research. “The Application of Geographic Information Systems” presents research findings that explain GIS’s applications in different subfields of social sciences. With several case studies conducted in different parts of the world, the book blends together the theories of GIS and their practical implementations in different conditions. It deals with GIS’s application in the broad spectrum of geospatial analysis and modeling, water resources analysis, land use analysis, infrastructure network analysis like transportation and water distribution network, and such. The book is expected to be a useful source of knowledge to the users of GIS who envision its applications in their teaching and research. This easy-to-understand book is surely not the end in itself but a little contribution to toward our understanding of the rich and wonderful subject of GIS.
    Pages: Online-Ressource (372 Seiten)
    ISBN: 9789535108245
    URL: http://www.intechopen.com/books/application-of-geographic-information-systems
    Language: English
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    Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach (2014)
    Warsaw, Berlin : De Gruyter Open
    Details
    Keywords: biochemistry ; laboratory ; lab ; lab manual
    Description / Table of Contents: Biochemistry laboratory manual for undergraduates – an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
    Pages: Online-Ressource (XII, 174 Seiten)
    ISBN: 9783110411331
    URL: http://www.degruyter.com/viewbooktoc/product/449852
    Language: English
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  • 14
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    Peopled Landscapes : Archaeological and Biogeographic Approaches to Landscapes (2012)
    Canberra : ANU Press
    Details
    Keywords: human ecology ; archaeology ; landscape assessment ; landscape changes ; humans ; nature ; Australia
    Description / Table of Contents: This impressive collection celebrates the work of Peter Kershaw, a key figure in the field of Australian palaeoenvironmental reconstruction. Over almost half a century his research helped reconceptualize ecology in Australia, creating a detailed understanding of environmental change in the Late Pleistocene and Holocene. Within a biogeographic framework one of his exceptional contributions was to explore the ways that Aboriginal people may have modified the landscape through the effects of anthropogenic burning. These ideas have had significant impacts on thinking within the fields of geomorphology, biogeography, archaeology, anthropology and history. Papers presented here continue to explore the dynamism of landscape change in Australia and the contribution of humans to those transformations. The volume is structured in two sections. The first examines evidence for human engagement with landscape, focusing on Australia and Papua New Guinea but also dealing with the human/environmental histories of Europe and Asia. The second section contains papers that examine palaeoecology and present some of the latest research into environmental change in Australia and New Zealand. Individually these papers, written by many of Australia’s prominent researchers in these fields, are significant contributions to our knowledge of Quaternary landscapes and human land use. But Peopled Landscapes also signifies the disciplinary entanglement that is archaeological and biogeographic research in this region, with archaeologists and environmental scientists contributing to both studies of human land use and palaeoecology. Peopled Landscapes reveals the interdisciplinary richness of Quaternary research in the Australasian region as well as the complexity and richness of the entangled environmental and human pasts of these lands.
    Pages: Online-Ressource (472 Seiten)
    ISBN: 9781921862724
    URL: http://press.anu.edu.au?p=165471
    Language: English
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    Facetten der Landschaftsinterpretation und des Tourismus (2014)
    Göttingen : Universitätsverlag Göttingen
    Details
    Keywords: landscape ; tourism
    Description / Table of Contents: Im vorliegenden Band werden ausgewählte Facetten der Landschaftsinterpretation und des Tourismus beleuchtet. Die angeführten Fallbeispiele fokussieren dabei nicht nur verschiedenartige Räume (z. B. Mittel- und Hochgebirge, pazifische Inselwelt), sondern sie entstammen auch mannigfaltigen Themenfeldern (z. B. Kultur- und Naturtourismus, Inszenierungsstrategien, Reiseerziehung). In ihrer Gesamtheit spiegeln die Beiträge der einzelnen Autorinnen und Autoren das breite Interesse tourismusassoziierter Forschung und Praxis wider. Trotz der inhaltlichen Vielfalt implizieren die Ausarbeitungen die Aspekte „Bildung“ und „Wissensvermittlung“ als Leitgedanken im Rahmen der nachhaltigen Inwertsetzung natur- und kulturräumlicher Ressourcen.
    Pages: Online-Ressource (178 Seiten)
    ISBN: 9783863951672
    URL: http://resolver.sub.uni-goettingen.de/purl?isbn-978-3-86395-167-2
    Language: German
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    Norlevinea n. g., a New Genus for Glugea daphniae (Protozoa: Microspore), a Parasite of Daphnia longispina (Crustacea: Phyllopoda) (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Norlevinea n. g. is established for microsporidia in which a uninucleate meront changes into a sporont by secreting a thin, membranous, sporontogcnetic and fragile sporophorous vesicle (pansporoblast membrane) in which four uninucleate sporoblasts are formed. In contrast to the genus Gurleya, the sporoblasts and later the spores are permanently joined into doublets, being laterally cemented by an electron-dense substance structurally identical to and continuous with the exospore layer. The polar filament is of the anisofilar type. The type species is Norlevinea daphniae (Weiser, 1947) n. comb., a parasite of the ovaries of Daphnia longispina occurring in several carp ponds in Czechoslovakia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb05492.x
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    Nosema helminthorum Moniez, 1887 (Microspora, Nosematidae):A Taxonomic Enigma (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . The microsporidian parasite known as Nosema helminthorum Moniez, 1887, parasitic in the tapeworm Moniezia expansa (Rudolphi, 1810), has been shown by electron microscopy to have two cycles of development, one with isolated nuclei, the other with paired nuclei (diplokarya). Both merogony and sporogony of the two separate sequences take place in direct contact with the host cell cytoplasm and ultimately give rise to unikaryotic and diplokaryotic sporoblasts. Sporogony is disporoblastic. The nuclear condition of the spores was not seen. The sequences, corresponding to those of the genera Unikaryon and Nosema, may be part of a single dimorphic life cycle and, if so, the species will have to be transferred to a new genus.
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    ABSTRACTS (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb05519.x
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    Essai ďApplication de ľAnalyse Phénétique à la Classification du Phylum des Ciliophora (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb05491.x
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    Description of Sarcocystis falcatula Stiles, 1893, a Parasite of Birds and Opossums (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Sarcocystis falcatula Stiles, 1893 is re-described. Intermediate hosts of the parasite which was earlier described as Sarcocystis debonei Vogelsang, 1929 are species of passeriform, psittaciform, and columbiform birds. In these birds, muscle zoites are 6.88 × 2.19 (4.8-8.4 × 1.2-3.6) μm and are enclosed in a cyst wall with regular protrusions, 1-5 μm long. The convoluted primary wall has multiple thin areas in the osmiophilic layer. Microtubules originate in the ground substance and extend to the tips of the protrusions. The only known definitive host is the opossum, Didelphis virginiana; rats, cats, a dog, and a ferret could not be infected from muscle cysts. Sporocysts from opossums infected from five different infected avian sources measure 11.2 × 7.4 (9.6−12.0 × 6.0-8.4)μm.
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    Parasitic Protozoa of the Blood of Rodents VI. Two New Hemogregarines of the Pygmy Flying Squirrel Idiurus macrotis (Rodentia: Theridomyomorpha: Anomaluridae) in West Africa (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Descriptions are given of two new species of Hepatozoon Miller, 1908 found in the pygmy squirrel, Idiurus macrotis, in the Ivory Coast. Gamonts of both are parasites of monocytes.The size and shape of the gamonts of one, H. normani n. sp., are similar to those of a number of gamonts of other species of rodent hemogregarines and the separate identity of the parasite is based on the host restriction of mammalian hemogregarines. The gamonts of the other species, H. dolichomorphon n. sp., are remarkably long and slender and are unlike those of any other known hemogregarine of mammals. Schizonts of this species were found in a smear prepared from heart blood.
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  • 22
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    Nonvariant Antigens Limited to Bloodstream Forms of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . The presence of nonvariant antigens (NVAs) limited to bloodstream forms of Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense was demonstrated for the first time by immunodiffusion and Immunoelectrophoresis. Noncloned and cloned populations were employed in preparation of polyclonal antisera in rabbits and of antigens to be used in the immunologic reactions. The NVAs could be shown best in systems in which hyperimmune rabbit sera (adsorbed with procyclic forms to eliminate antibodies against antigens common to bloodstream form and procyclic stages) were reacted with trypanosomes characterized by heterologous variant-specific antigens (VSAs).The NVAs demonstrated in this study are very likely different from the common parts of VSAs. As has been suggested by experiments with living trypanosomes, at least a part of the NVAs appears to be located on the surface of the bloodstream forms. In these experiments involving the quantitative indirect fluorescent antibody test, the amount of fluorescence recorded for the heterologous system, i.e. ETat 5 trypanosomes incubated with anti-AmTat 1.1 serum, equalled ∼3.0% of the fluorescence emitted by the AmTat 1.1 bloodstream forms treated with their homologous antiserum. Evidently, only small amounts of NVAs are present on the surfaces of T. brucei bloodstream forms.In addition to the NVAs, the electrophoresis results suggested the presence of antigenic differences between procyclic stages belonging to different T. brucei stocks.
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    Initial Extracellular Development in Vitro of Merozoites of Plasmodium falciparum (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Late schizonts from continuous cultures of P. falciparum were concentrated over Percoll, inoculated to various experimental media at the rate of about 20 × 106 per 0.5 ml of medium, and incubated in a candle jar at 37° for 1 day. Controls in standard culture medium showed a heavy invasion with young rings in the previously uninfected red cells introduced with the inoculum of schizonts. In a medium of high potassium content containing a 33% extract of human erythrocytes, this invasion was inhibited and many free merozoites were present. If, however, this same medium was supplemented with both ATP, as the dipotassium salt at 1.6 mM, and sodium pyruvate at 3.6 mM, there appeared large numbers of extracellular forms resembling young rings. Examination of these by electron microscopy shows that they are indeed merozoites that have begun to differentiate extracellularly. This suggests that the trigger for differentiation of merozoites may not depend on the process of entry into a red cell but rather on specific factors within the red cell.
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    ACKNOWLEDGMENT (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb05512.x
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  • 25
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    Besnoitia darlingi (Apicomplexa, Sarcocystidae, Toxoplasmatinae): Transmission between Opossums and Cats (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Opossums (Didelphis marsupialis), act as intermediate hosts for Besnoitia darlingi and could be infected orally with sporozoites (oocysts) and bradyzoites (tissue cysts), or intraperitoneally (i.p.) with tachyzoites. Infections could presumably be transmitted through cannibalism. Cats (Felis catus), the definitive host, could be infected only with bradyzoites but not sporozoites. Oocysts shed by cats measure about 12 × 12 μm, resemble similarly sized oocysts of Toxoplasma gondii and Hammondia hammondi, and must be differentiated by the appearance of tissue cysts after experimental infection of intermediate hosts. Cats did not form tissue cysts of B. darlingi. Tachyzoites from the related B. jellisoni could be used in the Sabin-Feldman dye test to determine the development of antibody to B. darlingi in opossums after infection.
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    ANNOUNCEMENT (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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    URL: http://dx.doi.org/10.1111/j.1550-7408.1984.tb05515.x
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  • 27
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    Extraintestinal Development of Caryospora simplex (Apicomplexa: Eimeriidae) in Experimentally Infected Mice, Mus musculus (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Developmental stages of Caryospora simplex were found in connective tissue of the cheek, tongue, and nose of Swiss-Webster and C57 BL/6 mice (Mus musculus) from 8 through 70 days after oral inoculation with 50,000 or 250,000 oocysts, or 60,000 free sporocysts of the same species obtained from an Ottoman viper, Vipera xanthina xanthina. The earliest developmental stages were seen on day 8 post-inoculation (PI) and consisted of two types of meronts and gamonts (undifferentiated sexual stages). Gamonts, microgametocytes, macrogametes, and unsporulated oocysts were found on days 10 and 12 PI. Fully sporulated, thin-walled oocysts containing eight sporozoites surrounded by a thin sporocyst membrane were first seen 12 days PI. Monozoic cysts (caryocysts) were first seen 12 days PI and appeared fully viable throughout the duration of the study, 70 days PI. Four mice injected intra-peritoneally with 150,000 free sporozoites and killed 12 days PI contained unsporulated and sporulated oocysts in connective tissues of the cheek, tongue, and nose, suggesting that sporozoites may be carried to the site of infection via the lymphatic/circulatory system. Four cotton rats, Sigmodon hispidus, inoculated orally with 250,000 oocysts all had unsporulated and sporulated oocysts of C. simplex in connective tissue of the cheek, tongue, and nose when killed on day 12 PI, indicating extraintestinal development in the secondary host is not species specific. This is the first report of a heteroxenous coccidium with both asexual and sexual development in the primary (predator) and secondary (prey) hosts.
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    Trypanosoma lucknowi, a New Species of Trypanosome from Macaca mulatta with Observations on Its Fine Structure (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Trypanosoma lucknowi n. sp. was isolated in culture from one of 126 Macaca mulatta originating from the vicinity of Lucknow, Uttar Pradesh, India. Trypanosoma lucknowi is distinctive because of the large number of epimastigotes and trypomastigotes which, in culture, exhibit no movement or only a slight bending of the flagellar end. This limited motility coincides with a free flagellum which is either completely absent or rudimentary. The microorganism is cloned readily, and the description is based upon such cultures. Trypanosoma lucknowi shows pronounced differences from other trypanosomes of South Asian macaques and from “aflagellar” African trypanosomes. The ultrastructural demonstration of a cytostome and contractile vacuole suggests ultimate grouping with stercorarian trypanosomes. A 3-D reconstruction of the flagellar pocket/cytostome region is included.
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    The Ultrastructure of the Freshwater, Colorless Dinoflagellate Peridiniopsis berolinense (Lemm.) Bourrelly (Mastigophora, Dinoflagellida), (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The ultrastructure of the freshwater, heterotrophic dinoflagellate Peridiniopsis berolinense (Lemm.) Bourrelly resembles other dinoflagellates in the structure of its nucleus, theca, flagella, and mitochondria. Other features less frequently reported in related organisms include fine sub-sulcal fibers, collared pits in the flagellar base region, and unusual structures herein termed fibrillar lamellae. Numerous vesicles are present, some of whose contents are distinctly crystalline, while others contain what appears to be membranous material arranged in either whorls or parallel stacks; still other vesicles contain electron-dense, granular spheres. Of particular interest is the transitional helix present in the longitudinal flagellum, this being the first report of such a structure among the dinoflagellates. Plastids of any kind are lacking, and a peduncle is present and is used during phagotrophy.
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    Entamoeba histolytica: Collagenolytic Activity and Virulence (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Several axenic strains of pathogenic and nonpathogenic Entamoeba histolytica were tested for their capacity to digest native radioactive type I collagen gels and to produce liver abscesses when injected into the liver of newborn hamsters. The results demonstrate that the pathogenic strains of amebas (HM1:IMSS, HM3:IMSS, HM38:IMSS, and HK9) have a collagenolytic activity that closely correlates with their in vivo capacity to produce liver lesions. The nonpathogenic isolate (Laredo) did not show collagenolytic activity and failed to produce lesions in the liver of newborn hamsters. The results also demonstrate that type I collagen obtained from rodents and cats is degraded less by amebic collagenase than is bovine collagen, which is similar to human collagen. These findings suggest that species susceptibility to invasive infection may depend, among other factors, on the characteristics of the extracellular components of host tissues.
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    Trypanosoma cruzi from the Paraguayan Chaco: Isoenzyme Profiles of Strains Isolated at Makthlawaiya (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: At Makthlawaiya, in the Paraguayan Chaco, the prevalence of Trypanosoma (Schizotrypanum) cruzi infection among both domestic Triatoma infestans and domestic dogs was 38%, and IgG anti-T. cruzi antibody was detected by the quantitative enzyme-linked immunosorbent assay (ELISA) in 80% (105/133) of human sera. Ninety percent (25/28) of T. cruzi strains isolated from both T. infestans and dogs showed heterozygous isoenzyme profiles for glucose phosphate isomerase, phosphoglucomutase and 6-phosphogluconate dehydrogenase. These strains appeared to be closely related to Bolivian zymodeme 2. Three Paraguayan T. cruzi strains showed homozygous isoenzyme profiles, similar to those of major Brazilian zymodemes. It was concluded that T. cruzi strains with heterozygous isoenzyme profiles predominate in domestic transmission cycles in this highly endemic area of the Paraguayan Chaco.
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    Localizing Surface-Related Proteins in Ciliates by Indirect Immunofluorescence Microscopy (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Results obtained in immunofluorescence localization studies involving three antisera, six species of ciliates, and a variety of fixation procedures suggest that superior results can often be obtained by fixing cells in 35–70% ethanol. Formaldehyde fixation appeared to induce redistributions of epiplasmic proteins and surface antigens which were not observed in ethanol-fixed cells. In addition, background fluorescence was significantly lower in ethanol-fixed cells than it was in cells fixed in aldehydes.
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    Actin in Tetrahymena paravorax: Ultrastructural Localization of HMM-Binding Filaments in Glycerinated Cells, (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Actin has been identified in the ciliated protozoon Tetrahymena paravorax on the basis of the ultrastructural detection of filaments typically decorated with heavy meromyosin (HMM) in glycerinated microstome cells. These filaments are widely distributed in endoplasmic and cortical regions and can form bundles. They are particularly numerous in elongating cells; HMM-binding filaments run approximately parallel to rib microtubules in the ectoplasm of the right wall of the buccal cavity and seem to extend to the cytopharyngeal region, suggesting some role of actin in maintenance of the crest-trough pattern of ribbed wall and/or in formation of food vacuoles. Extensive actin bundles are observed below some membranellar areas and are thought to follow the course of the microtubular “deep fiber bundle.” The “fine filamentous reticulum” underlying the oral ribs and the “apical ring” extending beneath kinetosomes of ciliary couplets display filaments that do not bind HMM and are ˜ 14 nm in diameter. No evidence for actin in these structures was obtained in the present study. The “specialized cytoplasm” of the cytostome-cytopharyngeal region appears as an undecorated reticulum with 20 nm-spaced nodes. Occasionally HMM-binding filaments were found inside the macronucleus, just beneath its envelope. Actin is suggested to be involved in cell shaping and in control of the transport of food vacuoles.
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    Altered Flagellar Size-Control in shf-1 Short-Flagella Mutants of Chlamydomonas reinhardtii (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two allelic Mendelian mutations which confer a short flagella phenotype were used to explore flagellar size control in Chlamydomonas reinhardtii. When mutant/wild type quadriflagellate dikaryon cells were constructed, their two short flagella rapidly grew out to near wild type length. The kinetics of elongation suggest that the flagellar assembly process is not intrinsically self-limiting as a number of otherwise attractive models for size control require. Instead, we suggest that there exists a cellular machinery dedicated to flagellar size control and that the short-flagella mutations alter the machinery in some as yet unknown way. One of the mutants shows temperature-sensitive flagellar assembly, and both are flagellaless in acetate media. Genetic analysis indicates that the temperaturesensitive, acetate-sensitive, and short-flagella phenotypes have a common genetic basis. The responsible gene has been named shf-1, and it has been mapped to chromosome VI, approximately 5 map units from the centromere.
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  • 35
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    A Comparative Study of 14 Strains of Naegleria australiensis Demonstrates the Existence of a Highly Virulent Subspecies: N. australiensis italica n. spp. (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Fourteen strains of Naegleria australiensis, including the type strain, were compared for virulence for mice, maximum growth temperature, lectin agglutination, isoenzyme pattern, and total protein banding pattern. Their relation to other species of Naegleria also was compared by immunoelectrophoretic analysis. Strains with high virulence, comparable to that of N. fowleri, were found to be different in concanavalin A agglutination as well as with regard to zymograms and total protein patterns. Although serologically different from N. fowleri and reacting with N. australiensis antiserum in the fluorescent antibody test, these high-virulence strains differed in number of immunoelectrophoretic precipitin bands. Because of these results, the high-virulence strains are considered to be a subspecies of N. australiensis. The low-virulence strains showed minor differences from the type strain. Thus, N. australiensis does not appear to be as homogenous a species as N. fowleri. Pathogenic N. australiensis also seems to be more widespread than previously thought.
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  • 36
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    New Aspects of the Morphology of Pleurotricha lanceolata (Ciliophora, Hypotrichida): Cirral and Membranellar Patterns and Fibrillar Systems (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In Pleurotricha lanceolata, the ventral somatic infraciliature presents 13 frontoventral cirri, 5 transverse cirri, one row with 18–19 left marginal cirri and two rows of right marginal cirri of different length. On the dorsal side there are six longitudinal rows of dorsal bristles, four of them bipolar and the other two less than half body length. The oral infraciliature includes the adoral zone of membranelles, with 45–55 membranelles of three or four rows of kinetosomes each, and two undulating membranes (paroral and endoral membranes), each with two rows of kinetosomes. Some structures of the oral and somatic fibrillar systems have also been examined and are similar to those described in other species of hypotrichous ciliates.
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  • 37
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    Eimeria beldingii n. sp. and Other Coccidia (Apicomplexa) of the Ground Squirrel Spermophilus beldingi (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Fecal samples of 36 ground squirrels, Spermophilus beldingi, from Tioga Pass (elev. ca. 3315 m) in the Sierra Nevada, California, yielded oocysts of Eimeria beckeri in nine squirrels, E. citelli in four squirrels, E. beldingii n. sp. in two squirrels, and degenerated, unidentifiable oocysts in ten squirrels. Eimeria beldingii n. sp. oocysts are ellipsoidal, 30–34 × 24–30 (mean 32 × 26) μm with a two-layered, rough, striated wall, without a micropyle or residuum, with polar granules; they contain ellipsoidal or ovoid sporocysts 11–15 × 9–12 (mean 13 × 10) μm with a Stieda body and residuum.
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  • 38
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    Paramecium Phagosome Membrane: From Oral Region to Cytoproct and Back Again (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ten years of research on digestive vacuoles (phagosomes) of Paramecium caudatum have revealed sequential changes both within the vacuole lumen as well as within the surrounding membrane. Four vacuole stages can be recognized by a combination of thin section and freeze-fracture ultrastructural features. Three sets of vesicles (discoidal vesicles, acidosomes, and lysosomes) fuse with the vacuole, each at a predetermined stage, to bring about these membrane and physiological changes. At various times membrane is removed as vesicles from the vacuole surface, which has the effect of regulating vacuole size. Membrane recycling, membrane replacement, and specific membrane to membrane recognition all appear to be operating during the digestive cycle. Details of these events are summarized in this address and a number of unanswered questions suggest areas for future research.
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    The Integrated Activity of Cilia: Function and Coordination (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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  • 40
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    Antigenic Variation in Trypanosomes Introductory Remarks of the Chairman (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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  • 41
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    Antigenic Variation in Trypanosomes Concluding Comment of the Chairman (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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  • 42
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    Taxonomy and Review of the Coccidian Genus Cryptosporidium (Protozoa, Apicomplexa) (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Reports of Cryptosporidium in various hosts and cross-transmission experiments are reviewed. Cryptosporidium has been found in mammals (Primates, Artiodactyla, Perissodactyla, Carnivora, Lagomorpha, and Rodentia), birds, reptiles, and fish. The only cross-transmission attempts that have been made have been from mammals to other mammals and to a few birds. Names have been given to 19 “species,” but it is concluded that only four of these should be considered valid at present. These are: C. muris Tyzzer, 1907 in mammals, C. meleagridis Slavin, 1955 in birds, C. crotali Triffit, 1925 in reptiles, and C. nasorum Hoover, Hoerr, Carlton, Hinsman & Ferguson, 1981 in fish.
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  • 43
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    In Vitro Excystation of Caryospora simplex (Apicomplexa: Eimeriorina) (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In vitro excystation of sporozoites of the heteroxenous coccidian Caryospora simplex Léger, 1904 (Apicomplexa: Eimeriorina) is described. Sporocysts freed mechanically from oocysts released a maximum of 51% of their sporozoites within 45 min at 25°C and a maximum of 74% within 20 min at 37°C when incubated in a 0.25% (w/v) trypsin–0.75% (w/v) sodium taurocholate (bile salt) excystation solution. At emergence from sporocysts, sporozoites were weakly motile then became highly active after about 2 min in excystation solution. Sporozoites within sporocysts exposed to bile salt only became highly motile within 25 min at 25°C and within 15 min at 37°C but did not excyst. When exposed only to trypsin at the above temperatures, the Stieda body dissolved; the substieda body remained intact, and the sporozoites exhibited only limited motility within sporocysts; only a few excysted. Intact, sporulated oocysts incubated at 25° or 37°C in 0.02 M cysteine-HC1 and a 50% CO2 atmosphere for 18 h had no morphologic changes in the oocyst wall. Further incubation of these intact oocysts in excystation solution for 30 min at 37°C caused neither motility of sporozoites within sporocysts nor excystation. Grinding oocysts for 30 sec in a motor-driven, teflon-coated tissue grinder caused motility of some sporozoites within sporocysts but did not result in excystation.
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    Inhibitory Effect of Rhodamine 123 on the Growth of the Rodent Malaria Parasite, Plasmodium yoelii (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The effect of the cationic permeant fluorescent dye, rhodamine 123 (R123), on the in vivo growth of Plasmodium yoelii was examined. Plasmodium yoelii-infected mouse erythrocytes were incubated in vitro with R123 and injected intravenously into mice. Examination of daily parasitemias showed that R123 delayed parasite growth whereas rhodamine 110, a neutral compound, and fluorescein, a negatively charged fluorescent dye, did not. Infected erythrocytes treated with R123 were not cleared from the circulation even 7 h after injection. Quantitation of cell-associated R123 by spectrophotometry revealed that infected cells with increased levels of R123 considerably prolonged the 2% prepatent period, the time required for the parasite to develop a 2% parasitemia. Degenerating parasites within and outside the host erythrocytes were observed on day 1 of infection in the mice. Thus it follows that R123, which accumulated in infected erythrocytes, inhibits the growth of P. yoelii; moreover, when R123-labeled infected erythrocytes were treated with 1–10 μM carbonylcyanide m-chlorophenylhydrazone (CCCP), a proton ionophore, to release R123 from the cells, the inhibitory effect on the growth rate of P. yoelii was partially reversed.
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    Caryospora corallae n. sp. (Apicomplexa: Sporozoea, Eimeriidae) from the Emerald Tree Boa Corallus caninus (Serpentes: Boidae) (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Oocysts of Caryospora corallae n. sp. were isolated from the feces of three Emerald Tree Boas Corallus caninus. The spherical oocysts of C. corallae averaged 22.4 μn (range 18.7 to 24.6) in diameter and were lacking a micropyle and oocyst residuum; a polar granule was present. The ovoid sporocysts measured 19.1(17.6-20.0) × 13.1(11.7-14.0) μm and a sporocyst residuum and a Stieda body were present. The oocyst wall was approximately 1 μm thick. The sporulation was completed in about 5–6 days at 23 ± 2°C. This is the first report of the genus Caryospora from Corallus caninus a member of the Boidae.
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    The Structure and Function of Cilia and Flagella. Introduction (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
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    The Generation of Ciliary Motion (1984)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 31 (1984), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
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  • 48
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    Tandemly Repeated Sequence Families in Micronuclear DNA of the Ciliate Stylonychia pustulata (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Topics: Biology
    Notes: One third of a collection of cloned Stylonychia pustulata micronuclear DNA PstI fragments were found to be of a similar size, consistent with their being members of a repetitious sequence family with a repeat size of about 160 base pairs. Cross-hybridization experiments confirmed that these small cloned fragments are related by sequence homology. Hybridization of the cloned repetitious sequences to PstI digested micronuclear DNA revealed a “ladder” of bands (step size = 160 base pairs), indicating that the repeats are found in tandem arrays. This is the first demonstration of highly repetitious, tandemly repeated sequences in a ciliated protozoan.
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  • 49
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    Stage-Dependent Effects of Chloroquine on Plasmodium falciparum In Vitro (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: The erythrocytic developmental cycle of Plasmodium falciparum can be conveniently divided into the ring, trophozoite, and schizont stages based on morphology and metabolism. Using highly synchronous cultures of P. falciparum, considerable variation was demonstrated among these stages in sensitivity to chloroquine. The effects of timed, sequential exposure to several clinically relevant concentrations of chloroquine were monitored by three techniques: morphological analysis, changes in the rate of glucose consumption, and changes in the incorporation of 3H-hypoxanthine into parasite nucleic acids. All three techniques gave essentially identical results. The trophozoite and schizont stages were considerably more sensitive to the drug than ring-stage parasites. Chloroquine sensitivity decreased as nuclear division neared completion. The increase in chloroquine sensitivity was coincident with a marked rise in the rate of glucose consumption and nucleic acid synthesis. The rate of nucleic acid synthesis decreased as schizogony progressed while glucose consumption continued at high rates during this process. The degree of chloroquine sensitivity was not highly correlated with either metabolic activity.
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  • 50
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    Trypanosoma rhodesiense Bloodstream Trypomastigote and Culture Procyclic Cell Surface Carbohydrates (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bloodstream trypomastigote and culture procyclic (insect midgut) forms of a cloned T. rhodesiense variant (WRATat 1) were tested for agglutination with the lectins concanavalin A (Con A), phytohemagglutinin P (PP), soybean agglutinin (SBA), fucose binding protein (FBP), wheat germ agglutinin (WGA), and castor bean lectin (RCA). Fluorescence-microscopic localization of lectin binding to both formalin-fixed trypomastigotes and red cells was determined with fluorescein isothiocyanate (FITC)-conjugated Con A, SBA, FBP, WGA, RCA, PNA (peanut agglutinin), DBA (Dolichos bifloris), and UEA (Ulex europaeus) lectins. Electron microscopic localization of lectin binding sites on bloodstream trypomastigotes was accomplished by the Con A-horseradish peroxidase-diaminobenzidine (HRP-DAB) technique, and by a Con A-biotin/avidin-ferritin method. Trypomastigotes, isolated by centrifugation or filtration through DEAE-cellulose or thawed after cryopreservation, were agglutinated by the lectins Con A and PP with agglutination strength scored as Con A 〈 PP. No agglutination was observed in control preparations or with the lectins WGA, FBA or SBA. Red cells were agglutinated by all the lectins tested. Formalin-fixed bloodstream trypomastigotes bound FITC-Con A and FITC-RCA but not FITC-WGA, -SBA, -PNA, -UEA or -DBA lectins. All FITC-labeled lectins bound to red cells. Con A receptors, visualized by Con A-HRP-DAB and Con A-biotin/avidin-ferritin techniques, were distributed uniformly on T. rhodesiense bloodstream forms. No lectin receptors were visualized on control preparations. Culture procyclics lacked a cell surface coat and were agglutinated by Con A and WGA but not RCA, SBA, PP and FBP. Procyclics were not agglutinated by lectins in the presence of competing sugar at 0.25 M. The expression of lectin binding cell surface saccharides of T. rhodesiense WRATat 1 is related to the parasite stage. Sugars resembling α-D-mannose are on the surface of bloodstream trypomastigotes and culture procyclics; n-acetyl-D-galactosamine and D-galactose residues are on bloodstream forms; and n-acetyl-D-glucosamine-like sugars are on procyclic stages.
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    Staining of Plasmodium yoelii-Infected Mouse Erythrocytes with the Fluorescent Dye Rhodamine 123 (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The cationic permeant fluorescent dye rhodamine 123 (R123) was used to stain Plasmodium yoelii-infected mouse erythrocytes. Fluorescence microscopic observations demonstrated that the parasite, but not the matrix of the infected erythrocyte, accumulated the dye. Differences in fluorescence intensity could not be found at the various developmental stages of the parasite; however, quantitation of the cell-associated dye revealed an increase in R123 uptake with parasite development. The retention of the parasite-associated dye, as measured by fluorescence microscopy and spectrophotometry after extraction of R123 with butanol, was markedly reduced by treatment of the infected erythrocytes with a proton ionophore, carbonyl cyanide m-chlorophenylhydrazone (CCCP), and an inhibitor of proton ATPase, dicyclohexylcarbodiimide (DCCD). These results indicate that the accumulation and retention of R123 in P. yoelii reflect the parasite membrane potential and suggest that the parasite plasma membrane has a membrane potential-generating proton pump.
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    INSTRUCTIONS TO JOURNAL OF PROTOZOOLOGY CONTRIBUTORS (1983)
    Oxford, UK : Blackwell Publishing Ltd
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    ACKNOWLEDGMENT (1983)
    Oxford, UK : Blackwell Publishing Ltd
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    Resting Cysts in the Ciliate Class Polyhymenophorea: Phylogenetic Implications (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: Distinctive organic-walled resting cysts of at least three different types with a highly conservative morphology appear to characterize specific orders or groups of genera within the Class Polyhymenophorea (Protozoa, Ciliophora), contrasting markedly with the great diversity of form seen in trophic stages. Polyhymenophorean ciliates have been considered in the past to form a cohesive class within the Phylum Ciliophora and, possibly, to represent the pinnacle of ciliate evolution. Evidence from cysts challenges the cohesive nature of the class, suggesting that the hypotrichs should be subdivided and that they have a different phylogenetic origin from the heterotrichs, tintinnids, and oligotrichs.
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    Fine Structure of Yellow-Brown Symbionts (Prymnesiida) in Solitary Radiolaria and Their Comparison with Similar Acantharian Symbionts (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Yellow-brown, algal symbionts varying in diameter from approximately 5 μ m to 20 μ m, associated with solitary Radiolaria with spongiose skeletons (i.e. Spongodrymus sp.), exhibit fine structural features resembling the Prymnesiida (botanical class, Prymnesiophyceae). A large central vacuole is surrounded by a thin layer of cytoplasm containing plastids with lamellae composed of three thylakoids and granular pyrenoids with internal tubules immersed between the thylakoids. The pyrenoids lack internal thylakoid membranes. The nucleus is surrounded by a dilated cisterna of the nuclear envelope that also encloses the plastids and gives rise to saccules of the endoplasmic reticulum. The algal symbionts appear coccoid; hence no flagella nor surface scales were observed. The symbiont fine structure is compared to similar yellow-brown symbionts associated with Acantharia. Thus far, three kinds of algal symbionts have been observed to be associated with solitary Radiolaria: dinoflagellate, prasinomonad, and this apparent prymnesiomonad.
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    Mitochondrial Associations with Specific Components of the Cortex of Tetrahymena thermophila Nanney & McCoy, 1976: Microtubules and the Epiplasm (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ultrastructural observations of the cortically-located mitochondria of Tetrahymena thermophila revealed associations not only between the mitochondria and certain of the cortical microtubule bands, but also between the mitochondria and the epiplasm of the cortex. Most of the distal mitochondrial surface is close and parallel to the epiplasm; favorable views show bridge-like structures spanning the 20–10 nm gap between the mitochondrion and the epiplasm.Previous studies have shown that the placement of mitochondria in the cortex appears to be determined by certain of the cortical microtubule bands. This study, however, shows that mitochondrion-microtubule interactions account for only a small proportion of the total mitochondrial area associated with the cortex; the rest is accounted for by the epiplasm. A possible analogue of the spectrin layer of erythrocyte membranes, the epiplasm may be important in helping to arrange the intricately organized components of the ciliate cortex. Its involvement in apparently helping to “moor” mitochondria to their cortical sites is the first suggestion of any role in cell patterning played by the epiplasm.
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    URL: http://dx.doi.org/10.1111/j.1550-7408.1983.tb01404.x
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    Caractéristiques Ultrastructurales de l'Hélioflagelle Tetradimorpha (Hsiung) et leur Intéret pour l'Etude Phyletique des Héliozoaires (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
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    Notes: Ce Tetradimorpha, rencontre en eau douce se présente soit sous forme sphérique pourvue de quatre flagelles et d'axopodes rayonnants, soit sous forme allongée avec a l'avant quatre flagelles associes a quatre axopodes et a l'arriére six a huit axopodes divergents. L'etude ultrastructurale révèle un cytosquelette axopodial de type centroplastidie comprenant un centroplaste lenticulaire homogéne, centre organisateur des quatre axopodes anterieurs et des six a huit axopodes posterieurs, auquel s'ajoutent les quatre cinetosomes des flagelles anterieurs. En outre, un deuxiéme éleément cytosquelettique incluant un microtubule associe chacun des quatre cinetosomes a l'axopode antérieur correspondant. Des cordons microfibrillaires réunissent axopodes et cinetosomes au niveau du centroplaste, puis a quelque distance du centroplaste les axopodes posterieurs. Les axonémes des axopodes comprenant de 5 a 30 microtubules sont constitues de triades, lorsqu'on peut détecter une organisation. Le noyau, a nucléole central est coince dans le cone axopodial posterieur, lui-méme entouré des dictyosomes. Par l'organisation du cytosquelette, par la structure des kinétocystes, par la structure des flagelles dépourvus de mastigonémes tubulaires, Tetradimorpha différe nettement de Ciliophrys marina. Comme le prévoyait Davidson (1975), il represönte bien un des chainons dans la série évolutive des Héliozoaires centrohélidiens. Mais il ne présente guère d'affinites avec les Chrysomonadines considerees comme la souche des Héliozoaires. L'intéret de ce Protiste dans l'étude de la differentiation et de l'evolution du cytosquelette est également présente.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACTThis freshwater species of Tetradimorpha has a spherical body with four flagella and radiating axopods; it transforms into a pear-shaped cell that anteriorly has four flagella intercalated between four axopods and posteriorly has six to eight divergent axopods. Ultrastructural study reveals an axopodial cytoskeleton of the centrohelidan type comprising an homogeneous lenticular centroplast which acts as MTOC for axopodial microtubules. A second skeletal element is a microtubular linkage between the kinetosomes and the axonemes of anterior axopods. A microtubule embedded in dense material diverges from near the base of each kinetosomes and parallels the distal portion of the axoneme of each anterior axopod. A microfibrillar envelope around the centroplast links the axopodial bases to the kinetosomes situated just above. Close to the centroplast, microfibrillar strands link the axopodial axonemes to the kinetosomes. Axopodial axonemes are composed of 5 to 30 microtubules irregularly arranged except for some that form equilateral triangles. The nucleus containing a central nucleolus is constrained within a cone formed by the axonemes of the posterior axopods and surrounded by dictyosomes. By the cytoskeletal organization, the structure of kinetocysts, and flagella wthout tubular mastigonemes, Tetradimorpha differs obviously from Ciliophrys marina. As Davidson (1975) predicted, Tetradimorpha is an intermediate link in the centrohelidan lineage: however, it lacks the characteristics of chrysomonads, the supposed ancestors of Heliozoa. The contribution of this genus to the study of the differentiation and the evolution of the cytoskeleton is also presented and discussed.
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    Biochemical Approaches to Mating Type Expression in Tetrahymena thermophila (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Two-dimensional gel electrophoresis was used to identify the patterns of protein synthesis during initiation, and the patterns of membrane protein expression following initiation, in all of the mating types of the Tetrahymena thermophila B family. In addition, one-dimensional analysis was used to survey 125I-Concanavalin A-binding proteins. Although a large number of proteins was identified by each technique, no variation among the mating types was observed.
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    Intracellular Bubble Formation: Differences in Gas Supersaturation Tolerances Between Tetrahymena and Euglena (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
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    Notes: Cells of Tetrahymena pyriformis, T. thermophila, and Euglena gracilis were saturated with nitrogen gas at pressures up to 300 atm and rapidly decompressed. Damage was assessed by measuring post-decompression cell fragmentation or viability. Occurrence of intracellular bubbles was determined by cinephotomicrography performed during the decompression or by direct observations afterwards. The extreme gas supersaturations induced led to intracellular bubble formation and rupture in cells of Tetrahymena that contained food vacuoles, but only with supersaturations of 175 atm or higher; 225 atm left few cells intact. Bubbles were never observed in cells of Euglena or in Tetrahymena cells freed of food vacuoles, even when they were decompressed from substantially higher nitrogen supersaturations. Cells of Euglena were most resistant and were unaffected by supersaturations up to 250 atm.
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    SMITHSONIAN INSTITUTION FOREIGN CURRENCY GRANTS PROGRAM- ANNOUNCEMENT (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
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    URL: http://dx.doi.org/10.1111/j.1550-7408.1983.tb05489.x
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    Macromolecular Syntheses During the Quick-Change Act of Naegleria (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: The rapid, synchronous differentiation of N. gruberi from amoebae to flagellates is a useful paradigm to study aspects of cell differentiation, including regulation of the synthesis of proteins that are related to the changes in cell shape and motility, which occur during differentiation. The differentiation requires synthesis of new RNA and protein molecules to accomplish defined morphogenetic events. Specific new proteins, including the tubulins that form the flagellar microtubules, are synthesized at various times during differentiation, and particular mRNA species appear and disappear. The time course of the synthesis of the α and β subunits of flagellar tubulin is paralleled by the programmed appearance and disappearance of flagellar tubulin mRNAs. The evidence supports the hypothesis that the synthesis of flagellar tubulin is regulated by the transcription, and subsequent disappearance, of flagellar tubulin mRNA. Translatable mRNAs for two calmodulin-like calcium-binding proteins appear and disappear contemporaneously with those for flagellar tubulin. During differentiation the synthesis of actin, the major protein of amoebae, is selectively shut down, and translatable actin mRNA rapidly disappears. This description of the orderly appearance, utilization, and disappearance of the mRNAs for actin, calcium-binding proteins, and flagellar tubulin during differentiation provides means and motivation to investigate the mechanisms that regulate these events.
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    Cortical Control over the Direction of Macronuclear Elongation in the Heterotrich Ciliate Stentor coeruleus (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Earlier experimental work involving macronuclear implants in Stentor coeruleus has shown that the cytoplasmic cortex of the nuclear site 1) attracts the macronucleus and 2) holds it in place during interphase. Now experiments indicate macronuclei transferred with overlying cortex elongate in the direction of the transferred cortical pigment stripes, whether or not the transferred stripes realign in the direction of the host stentor's stripes. Therefore the third function of the cortex is to determine the direction of elongation and thus assure that both daughter cells at division receive part of the macronucleus.
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    Appendages Associated with Glugea stephani, a Microsporidan Found in Flounder (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: During an electron microscopic study of Glugea stephani, three morphologically distinct tubular appendages that are continuous with the sporoblast plasmalemma were observed. The tubules were designated as: type I, 45–50 nm in diameter and 600–900 nm in length; type II, 25–35 nm in diameter, averaging 1300 nm in length; type III, 50–70 nm in diameter and with an indeterminate length, which often exceeds 3000 nm. Type III tubules contain regularly spaced, electron-dense particles that are approximately 30 nm in diameter. Since many genera of microsporida have some type of appendage, which may eventually be utilized for taxonomic purposes, we propose the formation of a system of serially numbered detailed descriptions of these structures to promote uniformity and clarity in future publications.
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    Conjugation Between G1 and G2 Phase Cells in Paramecium tetraurelia (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
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    Notes: Cells of Paramecium tetraurelia, stock hrd, cultured in a micro-capillary containing 1 μl fresh culture medium, expressed mating activity through the whole cell cycle. Mating-reactive G2 phase cells can conjugate with cells of other phases. The G2 phase cells, which have double (4C) the normal micronuclear DNA content, undergo pre-meiotic DNA synthesis when conjugated with G1 phase cells. The micronucleus of the progeny from the cross between a G1 and a G2 cell becomes triploid.
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    The Germination of Vavraia culicis Spores (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Buffered solutions of KCl and NaCl were tested for their stimulatory effect on the germination of variously-aged spores of Vavraia culicis. Germination was optimal in 0.2 M KCl, pH 6.5 for one isolate, and, for another isolate, peaks of germination occurred at pH 7.0 and 9.5. Spores incubated for several hours in suboptimal solutions became unable to germinate under optimal conditions. After being returned to water, they regained their ability to germinate. Calcium chloride, magnesium chloride, and ammonium chloride inhibited germination. After ingestion by mosquito larvae, spores germinated near the posterior end of the midgut.
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    Characteristics of an Uptake System for α-Aminoisobutyric Acid in Leishmania tropica Promastigotes (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: . Leishmania tropica promastigotes transport α-aminoisobutyric acid (AIB), the nonmetabolizable analog of neutral amino acids, against a substantial concentration gradient. AIB is not incorporated into cellular material but accumulates within the cells in an unaltered form. Intracellular AIB exchanges with external AIB. Various energy inhibitors (amytal, HOQNO, KCN, DNP, CCCP, and arsenate) and sulfhydryl reagents (NEM, pCMB, and iodoacetate) severely inhibit uptake. The uptake system is saturable with reference to AIB-and the Lineweaver-Burk plots show biphasic kinetics suggesting the involvement of two transport systems. AIB shares a common transport system with alanine, cysteine, glycine, methionine, serine, and proline. Uptake is regulated by feedback inhibition and transinhibition.
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    Methods for the Large-Scale Cultivation of an Oxytricha (Ciliophora: Hypotrichida) (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: . Oxytricha strains used in biochemical studies have traditionally been grown in unaerated, unagitated culture tubes or Fernbach flasks. These cultures are limited in volume to about one liter and have a very nonuniform distribution of cells, with the majority of the cells at the very top or bottom of the medium. We have found conditions in which Oxytricha can be grown in 50-liter fermentation vats. The cultures grow to a uniform density of about 6000 cells/ml.
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    The Fine Structure of the Internal Spiral of Kinetosomes in Foettingeria's Tomite, a Unique Storage Depot for Kinetosomes (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: . The fine structure of the tomite of Foettingeria actiniarum (Claparède) was examined and compared with that of other apostome tomites. This stage in the life cycle has a unique configuration of kineties that form a spiral through the cytoplasm in the interior of the body. The structure and behavior of this internal spiral were evaluated as a mechanism for the storage of kinetosomes, an adaptation to the ciliate's two-host life cycle. The spiral is composed of nine ribbons of laterally compressed kinetosomes that are in contact with a thin electron-dense fibril. Paralleling the kineties of the spiral are conspicuous, swollen lamellae of the rough endoplasmic reticulum; these lamellae contain moderately electron-dense material. The spiral is associated with the large contractile vacuole and winds about the macronucleus. The tomite of Foettingeria possesses a single, robust, caudal cilium located in a pit, along with the nozzle-like pore of the contractile vacuole. The walls of the pit contain several trichocysts arranged radially about the caudal cilium and aimed into the pit.
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    Ultrastructural Studies of a Vesicle System Associated with Endoplasmic Reticulum in Exo-Erythrocytic Forms of Plasmodium berghei (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: . Fine structural studies of a specialized vesicle system associated with the endoplasmic reticulum (ER) of exo-erythrocytic Plasmodium berghei suggest that this system may be the equivalent of a Golgi apparatus. Patches of ER, randomly distributed in the cytoplasm of developing parasites, are formed of smooth and ribosome-studded cisternae intermingled with each other. The vesicle systems are located between as well as at the edges of ER aggregates and appear to be in different stages of budding from the cisternae. Prolonged osmication reveals distinct staining of the nuclear envelope and ER of the parasites as well as part of the Golgi apparatus of the hepatocytes. However, the small vesicles associated with the parasite's ER are unstained, as are the coated vesicles in the Golgi region of the liver cell. These sites in the parasite cytoplasm seem comparable to the concave surface of the Golgi apparatus in liver cells. The pinched-off vesicles fuse with others to form the prominent peripheral vacuolization characteristic of the nearly mature exo-erythrocytic form. The formation of these peripheral vacuoles and their subsequent fusion with the parasite membrane may be an exocytosis mechanism supplying the rapidly expanding parasite with new plasma membrane material.
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    Fine Structure and Cytochemistry of the Nucleus and the Kinetoplast of Epimastigotes of Trypanosoma cruzi (1983)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 30 (1983), S. 0 
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    Notes: . Ultrastructural cytochemical techniques were used to analyze the nucleus and the kinetoplast of epimastigotes of Trypanosoma cruzi. With the use of ethanolic phosphotungstic acid, which detects basic proteins, reaction product was seen in the chromatin and at the periphery of the kinetoplast. Thallium alcoholate, which interacts with DNA, stained strongly the whole kinetoplast and the chromatin. With the use of a silver impregnation method that detects acidic nucleolar proteins, silver granules were seen preferentially located in the central region of the nucleolus. With the EDTA method, which reveals the presence of ribonucleoproteins, staining was observed in the nuclear pores. Also 6–8 nm fibrils, 25 nm and 40 nm granules, which correspond to the perichromatin fibers, interchromatin granules and the perichromatin granules, respectively, were identified in the nucleus. The EDTA method also revealed the presence of 40 nm granules in the kinetoplast. These granules were seen mainly at the two extremities of the kinetoplast. Freeze-fracture images indicate that the nuclear membrane contains ca. 9 pores/μm2 of nuclear surface area. The mean diameter of the pores was 80 nm. All these results suggest that epimastigotes of T. cruzi have a very active nucleus and a high rate of nucleocytoplasmic interchange.
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    Effect of Temperature on the Contents of the Two Energy-Reserve Substances, Paramylon and Wax Esters, in Euglena gracilis (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    Topics: Biology
    Notes: . When a streptomycin-bleached mutant of Euglena gracilis strain Z was cultured in the dark at 33, 26, or 15°C, the content of paramylon was higher at lower growing temperature while that of wax esters was higher at higher temperature. Transfer of the cells grown at 33°C–15°C decreased the wax ester content while increasing the paramylon content; transfer in the reverse direction caused reverse changes. On incubation with labeled acetate, the cells grown at 33°C showed more distribution of radioactivity in wax esters than the cells grown at lower temperatures. Apparently the two energy-reserve substances have different physiological functions.
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    ABSTRACTS (1982)
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    Ellobiophrya conviva n. sp., a Commensal of Marine Ectoprocts, and a Revision of the Family Ellobiophryidae (Ciliophora, Peritricha) (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    Notes: The sessiline peritrich Ellobiophrya conviva n. sp. is described from marine ectoprocts of the genus Bugula, the first report of an ellobiophryid on bryozoan hosts. The new species is distinguished from others of its genus by its different body proportions, size, host, and structure of the clasping holdfast (for which the new name cinctum is chosen). Ellobiophrya conviva has been found only on B. neritina and B. turrita and shows a marked seasonal cycle of abundance. The family Ellobiophryidae Chatton & Lwoff is revised on the basis of new information provided by E. conviva, with the single species of the genus Clausophrya removed to Ellobiophrya as E. oblida Naidenova & Zaika n. comb. The genus Caliperia Laird remains unchanged. The two genera of the revised family are distinguished from one another by differences in the structure of the cinctum. Hypotheses are advanced to explain the morphogenesis of the cinctum and the evolution of ellobiophryids from other peritrichs.
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    The Spatial and Temporal Distribution of Paramecium bursaria in the Littoral Zone (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    Notes: The spatial and seasonal distribution of Paramecium bursaria in two small Indiana ponds was studied using a sampling grid. Very small (5.0 ml) samples were taken so that the individual microhabitats could be studied. The results were evaluated in comparison to the data collected for the P. aurelia complex collected in the same manner and at the same sites. It was found that P. bursaria exist in a clumped distribution, but that the distribution was not very different from random. Paramecium bursaria also exist at the surface and at the mud-water interface. Temperature does not seem to play a statistically significant role in determining population size. The breeding system of P. bursaria is optimized for an outbreeding population of low density. In comparison, the species of the P. aurelia complex exist in a very clumped distribution, are found only at the mud-water interface, and are inbreeders. The evolutionary strategies of the two types of paramecia are discussed.
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    La Spore des Actinomyxidies: Synactinomyxon longicauda n. sp., un Nouveau Type de Groupement Sporal et Adaptations Planctoniques: Emission et Structure du Sporoplasme (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: L'étude du caractère planctonique de différentes spores d'Actinomyxidies montre une complexité croissante dans leur adaptation au milieu aquatique. Au contact de l'eau, les trois cellules épéisporales de chaque spore se transforment en flotteurs de forme différente suivant les espèces. Ces flotteurs peuvent s'unir entre eux en un style équivelent à un quatrième flotteur ou associer diversement les huit spores issues d'un měme pansporocyste. C'est le cas dans le genre Synactinomyxon dont la diagnose est modifiée pour inclure une deuxième espèce S. Iongicauda n. sp. Un type nouveau est décrit chez lequel la preéence d'ancres à l'extrémité des cellules épisporales permet de maintenir efficacement réunies plusieurs dizaines de spores émises simultanément. Nous avons observé dans les genres Aurantiactinomyxon, Synactinomyxon, Echinactinomyxon l'emission du sporoplasme. II est libére en entier et capable de se déplacer dans l'eau pendant plus d'une heure grǎce à des mouvements amoeboïdes. Chez Aurantiactinomyxon eiseniellae les études ultrastructurales montrent que l'enveloppe du pansporocyste, d'une part, les épispores et les capsules polaires d'autre part sont réalisées à partir de cellules distinctes et profondément modifiées. Quant au sporoplasme, autrefois décrit comme un plasmode avec de nombreuses paires de noyaux, il contient, en fait, des ensembles identiques dont chacun est constitué de l'union d'un noyau satellite et d'une cellule uninucléée.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACTThe study of the planktonic character of different Actinomyxidia spores reveals increasingly complex adaptations to an aquatic environment. On contact with water, the three episporal cells of each spore transform into floats, the forms of which differ according to species. These floats can join together so that a fourth type of float is formed, or they can unite in various ways the eight spores originating from the same pansporocyst. This is the case in the genus Synactinomyxon whose diagnosis is modified to include a second species S. Iongicauda n. sp. A new type is described in which the presence of anchors at the extremities of the episporal cells permits several dozen spores that have been emitted simultaneously to be kept together. We have observed the emission of the sporoplasm in the genera Aurantiactinomyxon, Synactinomyxon, and Echinactinomyxon. It is freed completely and for more than an hour is capable of changing its position in the water by amoeboid movements. In the case of Aurantiactinomyxon eiseniellae, ultrastructural studies show that the pansporocyst envelope on the one hand, and the epispores and polar capsules on the other hand, are formed from separate but profoundly modified cells. The sporoplasm, however, sometimes described as a plasmodium with numerous pairs of nuclei, contains, in fact, identical complexes, each consisting of a uninucleate cell united with a satellite nucleus.
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    Kinetic Analysis of Chemokinesis of Paramecium (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Paramecia detect and accumulate in or disperse from some chemicals. Cells do this by changing frequency of turning and speed of swimming. There are at least two mechanisms by which cells respond: one dependent on ability to turn, one dependent on speed modulation. There are also two classes of chemicals: those that require the cells' ability to turn in order to cause accumulation and dispersal (type I), and those that apparently require only speed modulation (type II). Attractants of type I cause qualitatively similar changes in behavior to repellents of type II and the converse; therefore, assays are needed to distinguish between these two classes of chemicals, despite qualitatively similar behavior of some attractants and repellents. We examined two assays of paramecium chemoresponse, T-maze assay and well test, to understand how the T-maze distinguishes between attractants of type I and repellents of type II and why the well test does not.
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    Spore Germination in the True Slime Mold Didymium nigripes (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The morphology of spore germination in Didymium nigripes was studied using scanning electron microscopy and Nomarski phase optics. First, the outer spore wall splits, revealing a fibrillar inner wall. Remnants of the inner wall continue to cover the newly emerged amoeba. A single nucleus and a prominent vacuole are visible throughout germination. Germination is more rapid in glucose-peptone-yeast extract than in phosphate buffer. Germination is completely inhibited at 4°C, and is very slow at 18°C. Germination is most rapid at 26°C; at 21°C or 32°C it is slightly slower. Germination is reversibly inhibited by 20 μ/ml cycloheximide, but not by 200 μ/ml 5-fluoro uracil or 200 μ/ml proflavin. It is completely inhibited by 10-3 M Na azide.
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    Effect of Temperature and Osmolarity on Growth of Crithidia fasciculata, C. hutneri, C. luciliae thermophila, and Herpetomonas samuelpessoai (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Crithidia fasciculata (Anopheles, Culex, and Nöller strains), C. hutneri, C. luciliae thermophila, and Herpetomonas samuelpessoai were grown in a defined medium with different values of osmolarity at different temperatures. C. fasciculata (all strains) grew best between 300 to 500 mOsm; H. samuelpessoai, 400–500 mOsm; and C. hutneri and C. luciliae thermophila, 500–800 mOsm. At higher temperatures better growth was obtained at the upper osmolarities.
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    In Memoriam: WILLIAM BALAMUTH (1914–1981) (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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    URL: http://dx.doi.org/10.1111/j.1550-7408.1982.tb02872.x
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    Distribution of Members of the Crypthecodinium cohnii (Dinophyceae) Species Complex (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Crypthecodinium cohnii, a small marine heterotrophic homothallic dinoflagellate, has diversified into a complex of morphologically very similar breeding groups (biological species or sibling species), some of which have become widely dispersed. Membership of two clones in the same sibling species is shown by their sexual compatibility as determined by genetic complementation in zygotes formed from motility mutants derived from the two stocks. Membership in different sibling species may be inferrec when motility mutants of one strain do not complement those of another. Fifty-six clones representing seaweed enrichments from *** geographic sites have been found to belong to 28 sibling species; 35 clones are members of seven wide-ranging biological species, and 21 are single representatives of 21 other breeding groups within the ranges of the others. Of 174 clonal isolates in our possession, 168 conform in size and shape to C. cohnii. Six others which have smaller cells and only one-fifth the standard DNA and chromosome number belong, we believe, to another species. The C. cohnii complex provides a unique opportunity for the study of evolutionary divergence and geographical dispersion of a dinoflagellate.
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    Genus Pleistophora Gurley, 1893: An Assemblage of at Least Three Genera (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Until recently, pansporoblastic microsporidia that produce a variable and large number of sporoblasts from a sporont have been included in a single genus, namely Pleistophora Gurley, 1893. Ultrastructural studies have been used to determine whether the resemblance of these species is fundamental or superficial. The results indicated that the multisporous pansporoblastic forms belong to at least three genera and, thus, that Pleistophora is a “composite genus.” The term pansporoblast was originally used for stages in myxosporidian development. The term sporophorous vesicle adopted from Gurley is suggested for the spore-containing vesicle in the Microspora. Three species were studied: Pleistophora typicalis, the type-species; Pleistophora culicis, for which a new genus Vavraia has already been proposed; and Pleistophora simulii. P. typicalis and V. culicis have isolated nuclei throughout their development, and the sporophorous vesicle wall enveloping the sporoblasts is derived from amorphous secretions laid down during merogony external to the plasmalemma. Pleistophora and Vavraia are differentiated principally in terms of the structure of the sporophorous vesicle wall and mode of division of the sporogonial plasmodium. The nuclei of young sporonts of P. simulii are in diplokaryon arrangement and undergo meiosis to give haploid nuclei in the sporoblasts. The sporophorous vesicle wall is membranoid and is laid down external to the plasmalemma at the onset of sporogony. A new genus, Polydispyrenia n. g., is suggested for this species, the affinities of which are closer to the dimorphic species of microsporidia than to Pleistophora or Vavraia. The terms “merontogenetic sporophorous vesicle” and “sporontogenetic sporophorous vesicle” are used to distinguish between the two groups.
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    Effects of Malaria (Plasmodium berghei) on the Maternal-Fetal Relationship in Mice (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Plasmodium berghei infection was more severe in pregnant than in nonpregnant mice. Infection initiated on gestation day 7 resulted in rapidly increasing parasitemia and deaths of all pregnant mice within 12 days, while some nonpregnant mice survived until day 21 postinfection. When mice were infected on gestation day 12 or 14, a proportion of mice died before parturition; but some animals survived to deliver living pups. Reduced birthweights and increased spleen weight to body weight ratios were seen in pups from infected mice as compared with pups from uninfected animals. Histopathological abnormalities of placentae from infected animals included degeneration of the normal labyrinthine architecture and thickening of the trophobast separating maternal and fetal blood vessels.
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    Biochemical Characterization of Secreted Proteases During Growth in Tetrahymena pyriformis WH-14: Comparison of Extracellular with Intracellular Proteases (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Tetrahymena pyriformis strain WH-14 secreted large quantities of intracellular proteases into its culture medium during growth. Extracellular enzymes were purified to homogeneity from cell-free medium by ammonium sulfate precipitation, CM-Sephadex column chromatography, gel filtration, and DEAE-cellulose column chromatography. The DEAE-cellulose eluates were separated into four peaks (P-I, P-II, P-III, and P-IV), each of which exhibited a different specific activity toward azocasein and α-N-benzoyl-DL-arginine-ρ-nitroanilide (Bz-Arg-Nan). These four forms of the protease showed similarity in amino acid composition, molecular weight (21,000–24,000), and antigenic reactivity. They had pH optima at neutral range. P-I showed the highest specificity to azocasein whereas P-IV was most effective toward the synthetic substrates. The Km values for hydrolysis of Bz-Arg-Nan were 2.4, 1.6, 1.3, and 1.4 mM for P-I, P-II. P-III, and P-IV, respectively, and the corresponding Kcat/Km values were 5.0, 9.4, 28.5, and 114.3 S-1.M-1. These properties of secreted proteases were compared with those of intracellular proteases purified by the same procedure except for the initial Triton X-100 extraction. There were similarities in specific activity toward two substrates, molecular weight, Km, pH optima, and antigenic reactivity between the proteases from two sources, providing evidence that the intracellular proteases may be secreted into the extracellular medium without modification.
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    Separation of Individual Stages of Trypanosoma cruzi Grown in Cell Culture by Continuous Free-Flow Electrophoresis (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The separation of extracellular protozoan parasites from host cells based on a difference in surface charge has been described. However, with Trypanosoma cruzi no method exists for the isolation of pure parasite stages from heterogeneous mixtures. Studies on the electrophoresis of mixed stage populations confirm significant surface charge density differences exist among epimastigotes, trypomastigotes, and amastigotes. In ascending order of electronegativity, amastigotes have the lowest charge density, try-pomastigotes next, followed by epimastigotes. A technique has been developed for the separation of purified populations of parasites based on these charge differences using a continuous free-flow electrophoresis apparatus. The separated populations are morphologically intact and maintain their infectivity to mice. This separation method is applicable for preparative and analytical isolation of pure stages of T. cruzi for biochemical and immunological studies.
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    Changes in Two Acid Hydrolase Levels During Cyst Differentiation of a Ciliate, Histriculus muscorum (1982)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 29 (1982), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cellular levels of protein and two acid hydrolases, acid phosphatase (EC 3.1.3.2) and acid proteinase, were followed during cyst differentiation, arbitrarily divided into five stages, in the ciliate Histriculus muscorum Kahl. Extracellular enzyme activities were also measured. Protein content decreased gradually during cyst differentiation. In mature cysts the protein content was ca. 60% that of stationary phase organisms. The activities of both acid hydrolases remained unchanged during stage 1 and then decreased gradually; acid proteinase decreased more rapidly. Both enzymes remained slightly active in the mature cysts. The acid proteinase activity of stage 1 was reduced by cycloheximide treatment at time zero, whereas the enzyme was no longer sensitive to the inhibitor when treated at 1.5 h (late stage 1) after the first wash with encysting medium. Acid phosphatase activity was insensitive to the inhibitor. Extracellular release of acid phosphatase increased linearly at least until stage 5, although the extracellular release of acid proteinase was not detected. Cycloheximide blocked the extracellular release of acid phosphatase after stage 1. These results suggest that de novo synthesis of acid proteinase occurs during stage 1 and that lysosomes may play an important role during early stages of cyst differentiation.
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    The Dynamics of Micronuclear Mitosis in the Living Ciliate Spirostomum teres as Revealed by Polarization Microscopy (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Micronuclear mitosis in living Spirostomum teres has been studied by sensitive polarization microscopy, and the dynamic aspects of micronuclear division are described. The small, spherical, interphase micronuclei lie in form-fitting depressions in the macronuclear surface. Nuclear division begins with the rounding and slight swelling of the macronucleus and, coincidentally, the micronuclei move out of the depressions and away from the macronucleus, increase in size, and become weakly birefringent. As mitosis proceeds, the micronuclei increase in uniaxial birefringence and elongate to form irregular ovoids that convert to angular structures displaying principal axes of positive birefringence so divergent as to appear oriented at a right angle to one another. Micronuclei maintain this appearance for as long as 60 min and then abruptly change to rectangular-shaped structures, increase in uniaxial birefringence, and begin anaphase elongation. The somewhat dumbbell-shaped micronuclei lengthen at the constant rate of 2.0 μm/min to reach lengths 〉70 μm. It appears that little half-spindle shortening occurs during spindle elongation. Accompanying the changes in micronuclear spindle length are changes in birefringence. Just before elongation begins, presumably metaphase, the micronucleus is uniformly and intensely birefringent. At the magnifications employed, a chromosome plate is not clearly visible as a region of reduced birefringence. As elongation begins, the putative half-spindles are more birefringent than is the interzone, a condition that is maintained until the spindles have achieved ∼30% elongation, at which time a region of increased birefringence develops at the center of the interzone. This pattern persists for a very short time, then gives way to a uniform birefringence of the entire separation spindle that is maintained until elongation is completed. The rate of micronuclear spindle elongation, changes in micronuclear dimensions, and corresponding changes in birefringence are discussed with respect to possible mechanisms of mitosis.
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    Occurrence of Amoebae In and Around the Mushroom Laccaria trullisata (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Studies performed with the basidiomycete Laccaria trullisata collected from the sandy beach at the Hempstead Lake State Park, Long Island, New York, during the growing seasons of 1979 and 1980, have demonstrated a carposphere (equivalent to rhizosphere) effect. This region exerts a positive influence on the population density of amoebae when numbers are compared with those obtained in the bare sand 5 cm away. Moreover, amoebae have been shown to exist in, and have been recovered from, internal tissue of the cap (72%) and stalk (91%) of these mushrooms. A partial characterization of three strains of amoebae isolated from the internal tissue of L. trullisata and established in clonal culture is presented.
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    Données Nouvelles sur Quelques Processus Morphogénétiques chez les Hypotriches, Notamment dans le Genre Euplotes: Leur Contribution à l'Approche Evolutionniste du Problème de la Régulation de l'Activité Morphogenétique chez les Ciliés (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: L'étude, par le protargol, des phénomènes infraciliaires et de leur corrélation avec les phénomènes nucléaires au cours de l'autogamie dans le genre Euplotes montre, par comparaison avec la conjugaison, que les diverses étapes de la morphogenèse sont liées à la progression de l'état nucléaire. Par ailleurs, l'étude comparative des différents types de morphogenèse (bipartition, phénomena sexuel, réorganisation induite par le jeǔne) permet de supposer qu'il existe deux territoires morphogènes soumis à des systèmes de régulation bien distincts. La comparaison des séquences de morphogenèse chez divers hypotriches conduit à dresser un plan général d'évolution de la régulation de l'activité corticale en relation avec l'étendue des remaniements associés à la stomatogenèse.〈section xml:id="abs1-2"〉〈title type="main"〉ABSTRACTThe changes in the arrangement of the infraciliature associated with autogamy in Euplotes are described and compared with similar events associated with conjugation. The successive steps of morphogenesis are strongly correlated with nuclear processes. The comparative study of different types of morphogenesis (binary fission, sexual phenomena, starvation-induced reorganization) leads to the hypothesis that two morphogenetic fields (a ventral one and a dorsal one) depend on separate regulatory systems. From the viewpoint of evolution, the morphogenetic sequences of some hypotrichs have been compared. A general scheme of the evolution of cortical regulation is proposed, taking into account the extension of the area concerned with stomatogenic activity.
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    Histological and Ultrastructural Studies of the Interaction of Toxoplasma gondii Tachyzoites with Mouse Omentum in Experimental Infection (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    Topics: Biology
    Notes: Mouse omentum was studied after intraperitoneal challenge with tachyzoites of Toxoplasma gondii. Parasites inhabit omental histiocytes, fibroblasts, mesothelial cells, and free peritoneal macrophages. Recently infected cells showed enhanced metabolic and functional activity. Villous projections of the parasitophorous vacuole wall appeared, usually opposite the anterior pole of the parasite. In mesothelial cells, projections formed terminal swellings not observed in other infected cells. Activation of host cells was followed by reduction of the density of the cytoplasmic matrix, autophagosome formation, and intracellular edema, indicating the damage. The wall of the parasitophorous vacuole loses the supporting host cell endoplasmic reticulum that was attached to the vacuole just after entrance of the parasite into the cell. Then lysis of the parasitophorous vacuole and complete cell destruction occurs. The growth of parasites in undamaged cells does not coincide with the inflammatory response. Inflammation of the peritoneum develops only after the start of mass destruction of infected cells. Thus tachyzoites of Toxoplasma exert significant pathogenic effects by their ability to activate the host cell, causing lysis of the parasitophorous vacuole and subsequent destruction of the entire cell.
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    In Memoriam: TRACY M. SONNEBORN (1905–1981): A Personal Panegyric (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02819.x
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    In Memoriam: ASA ARTHUR SCHAEFFER (1883–1980) (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
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    Electrically Induced Tentacle Retraction in the Suctorian Protozoon Discophrya collini (Root) (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    Topics: Biology
    Notes: Discophrya collini is a free-living suctorian with retractile tentacles covered by a thick fibrous cortex. The tentacles contain a microtubular central canal surrounded at the base by a fibrous collar. Electrical stimulation induces a reproducible tentacle retraction. With extracellular electrodes, the tentacles nearest the anode respond initially, contracting by up to 75% of their original length. There is an inverse relationship between voltage level and duration of stimulus in producing a threshold response, and at a set voltage, between duration and degree of retraction. With intracellular electrodes, the membrane potential has been measured as -30 mV, and tentacle retraction occurs in response to as little as 1.25 nA when the intracellular electrode is made the cathode of the circuit. SEM studies show that retracted tentacles have a wrinkled cortex, while TEM shows that the microtubular canal bends as it enters the cytoplasm. No consistent changes occur in the microtubule configuration of the canal on retraction, suggesting that the microtubules are not directly involved in the contractile mechanism.
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    Rapid In Vitro Prescreen for Chelators as Potential Trypanocides Based on Growth of Crithidia fasciculata (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A rapid in vitro prescreen for Fe-binding chelators has been developed with growth of Crithidia fasciculata and the sparing of its heme requirement in a defined medium as a test system. The prescreen functions as an index of chelator-mediated Fe transport and as an index of growth inhibition, presumably by the interference with Fe and/or heme metabolism at intracellular chelatable sites. Of 161 chelators examined, 84 were active heme-sparers; 32 of these inhibited growth at low chelator concentrations. Twenty-eight other chelators inhibited growth and another 49 were inactive. Such chelating activity directed at Fe and heme targets in hemoflagellates may provide leads for chemotherapy.
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    Determination of Maximal Growth Rate Using Continuous Culture (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
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    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Study of microorganism growth kinetics requires measurement of maximal specific growth rate. Standard methods of measurement-batch, semicontinuous and continuous steady state-have sources of imprecision that can be substantially reduced by a modification of the continuous steady-state method. Data are presented, using the ciliate Tetrahymena pyriformis, that indicate that the theoretical foundation of the new method is firm and that precision can be increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02821.x
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  • 95
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    Identification of Surface Proteins on Viable Plasmodium knowlesi Merozoites (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Viable merozoites of Plasmodium knowlesi were isolated and the proteins that were labeled on intact merozoites by lactoperoxidase-catalyzed radioiodination were identified. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography of Triton soluble extracts of labeled merozoites demonstrated eight major bands ranging in apparent molecular weight from 150,000 D to 22,000 D. Exposure of intact merozoites to trypsin (10 μg/ml) for 10 min resulted in the loss of the two highest molecular weight proteins (150,000 D and 105,000 D) and the appearance of two new bands at 70,000 D and 62,000 D. Trypsin treatment under these conditions also removed the receptor(s) for merozoite attachment to erythrocytes. Therefore, these high molecular weight proteins are candidates for the merozoite component that attaches to erythrocytes. There was no evidence that the labeled membrane components were serum or erythrocyte membrane components, two potential contaminants in the preparation. Anti-rhesus erythrocyte antibody did not precipitate labeled merozoite proteins. Furthermore, the immunoprecipitation of labeled merozoite proteins by rhesus anti-merozoite serum was not inhibited by erythrocyte ghosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02825.x
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  • 96
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    Ultrastructural Differences Between Species of Trypanosomatids With and Without Endosymbionts (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Species of trypanosomatids without endosymbionts (Leptomonas seymouri, L. collosoma, L. samueli, Crithidia fasciculata, C. luciliae, C. acanthocephali, Herpetomonas megaseliae, H. mariadeanei, H. samuelpessoai, H. muscarum muscarum, Trypanosoma cruzi) and species of trypanosomatids with endosymbionts (Crithidia deanei, C. oncopelti, Blastocrithidia culicis) were comparatively studied by means of electron microscopy. Artificially aposymbiotic strains derived from species with symbiont were also included in the survey. Species with symbiont were found to differ in some ultrastructural aspects from the group of species without symbiont. Paraxial rods of flagella or intraflagellar structure were found exclusively in species without symbiont. Peripheral branching of mitochondria, accompanied by absence of subpellicular microtubules in sites where the mitochondrial branches are appressed to the cell membrane, were found exclusively in species with symbiont. Networks of kinetoplast DNA fibrils were found to be larger and looser in species with symbiont. Symbiont-free strains of species with symbiont retained the same morphological characteristics of their parental species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02829.x
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  • 97
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    Particle Assemblies in the Plasma Membrane of Tetrahymena: Relationship to Cell Surface Topography and Cellular Morphogenesis (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: During a freeze-fracture electron microscopical study of the plasma membrane of Tetrahymena, several different types of organized particle assemblies were observed. Three of these were found only on the protoplasmic face and were localized in the anterior-ventral region of the cell. These consisted of plate-like arrays composed of 4–25 triplet rows of small 3–4 nm particles; long, paired linear arrays localized at the tops of cortical ridges and composed of 7–8 nm particles; and elongated tetragonal arrays located in the grooves between ridges and composed of approximately 10 nm particles. The distribution of these arrays is consistent with roles in cellular morphogenesis, chemoreception, or cell-cell pairing during conjugation. In addition, a unique particle track associated with the cytoproct (anal pore) was observed in the external face of the plasma membrane. Furthermore, the protoplasmic face of the plasma membrane is characterized by a high density of particles organized into localized microarrays, consisting of small paracrystals or strings, which exhibit a loose higher-order patterning most evident toward the anterior end of the cell. Particle distributions on the protoplasmic face do not appear to be significantly altered by conditions that cause clumping of alveolar membrane particles. Taken together, these observations are consistent with the idea that the proteins of the plasma membrane are highly ordered and relatively immobile and that the structure of the plasma membrane is regionally differentiated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02832.x
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  • 98
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    Dactylosoma hannesi n. sp. (Dactylosomatidae, Piroplasmia) Found in the Blood of Grey Mullets (Mugilidae) from South Africa (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . A new species of Dactylosoma (Dactylosomatidae, Piroplasmia), for which the name Dactylosoma hannesi n. sp. is proposed, was discovered in blood erythrocytes of Mugil cephalus, Liza richardsoni, and L. dumerili (Mugilidae) from Swartkops estuary, located east of Port Elizabeth, South Africa. The life cycle of this species differs in some respects from that described for all other known species of Dactylosoma and Babesiosoma. Mature schizonts contain eight nuclei but undergo division only to two to four daughter cells. During cytoplasmic cleavage, schizonts assume triad, rosette, or cruciform shapes. Merozoites are finally produced through a series of binary fissions of these daughter cells which may also be involved in additional nuclear divisions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05325.x
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  • 99
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    Titles of Recent Books Received for Review (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb05328.x
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  • 100
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    Ultrastructure of the Flagellar Apparatus and Attachment of Herpetomonas ampelophilae in the Gut and Malpighian Tubules of Drosophila melanogaster (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Electron microscopy was used to examine the flagellar apparatus of Herpetomonas ampelophilae from the gut and malpighian tubules of Drosophila melanogaster. The flagellates attach to the microvilli either by weaving their flagella between the microvilli or by engulfing several microvilli with an external flagellar membrane. The first type predominated in the gut while the second type was limited to the malpighian tubules. Desmosomes were not involved in either type of attachment. A subpellicular collar with emerging microtubules was found to be adjacent to the desmosome of the flagellar pocket of herpetomonads in the gut.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02854.x
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