ALBERT

Description: The morphological features associated with Co-rich manganese deposits, the size variations of nodules, and the occurrence of different substrates have been analyzed, to evaluate the influence of various seabed slope angles on the distribution of these features. The coverage and size of the crusts depend on their surface morphology and seabed topography, resulting in cobble-type, lineated, or step-like outcrops. Small nodules (1–4 cm in diameter) dominate all seabed slopes, with a few locations having nodules ranging from 1 to 8 or 1 to 10 cm. Sediments invariably occur as substrates for nodules and as cover for crusts, their coverage being inversely proportional to that of the nodules and crust outcrops.

Description: Biomineralized structures and tissues are composites, containing a biologically produced organic matrix and nano- or microscale amorphous or crystalline minerals. Two main examples of organic matrices – the amino-polysaccharide chitin and the asymmetric protein collagen – are presented and discussed as the basic structural modules and organo-templates for calcium and silica biomineralization in nature. Both serve as templates, providing preferential sites for nucleation and controlling the location and orientation of mineral phases. Here, for the first time, chitin and collagen are analysed from evolutionary, structural, and functional points of view with respect to their templating properties in calcification and silicification phenomena, using both in vivo and in vitro data. It is proposed that these biopolymers be characterized as fundamental templates in biomineralization, inasmuch as they are very ancient from an evolutionary point of view, common to many species and biological systems with a global distribution. The two polymers also exhibit very similar hierarchical structural organizations, in spite of the possible alternatives they provide in chemical nature and origin. In addition, the phenomenon of multi-phase mineralization – where two minerals, amorphous and crystalline CaCO3, form from one biomolecule, chitin – is also described, analysed, and discussed for the first time.

Description: Despite coelacanths, Latimeria chalumnae, being listed as either endangered by CITES or critically endangered by the IUCN, their population size within South Africa is unknown and still needs to be estimated. Their conservation status unfortunately excludes the use of conventional tagging to mark individual animals for a possible mark–recapture experiment. This study shows that because coelacanths have a unique spot patterning it is possible to quickly and accurately identify specific individuals photographically using computer-aided identification software. Without any manual intervention by an operator, the software accurately identified between 56 and 92% of the individuals. Indentification success increased to 100% if the operator could also manually select from other potential matching photographs. It was also shown that fish exhibiting a yaw angle not exceeding 60° could be accurately identified in photographs, although the percentage of fish correctly identified without operator-intervention decreased rapidly with increasing yaw angle. Computer-aided identification should therefore facilitate future coelacanth research as it is both efficient and accurate while also reducing potential stress on the animals observed.

Description: The deep ocean floor between the Clarion and Clipperton fracture zones (NE equatorial Pacific) has the highest known manganese nodule abundance in the world oceans. A detailed analysis of MR1 (Mapping Researcher 1, 11–12 kHz) sonar images and free-fall grab data in the Korean manganese nodule field areas reveals a close relationship between side-scan sonar characteristics of the seafloor and manganese nodule abundance. Eight sonar facies are identified based on back-scattering intensity and distribution patterns. These sonar facies can be interpreted as (1) volcanic seamounts (facies I-1), (2) bounding faults of abyssal hills (facies I-2 and II-1), (3) lava flows or volcanoclastic mass-flow deposits around the volcanic seamounts (facies I-3 and II-2), (4) crests of abyssal hills (facies II-1), (5) abyssal troughs between abyssal hills (facies III-1), (6) relatively flat areas (facies II-3 and III-2). In the areas where facies II-1 (abyssal hill crests with thin sediment cover) and II-3 (relatively flat areas draped by thin sediments) are dominant, manganese nodules occur abundantly. In contrast, zones comprising facies III-1 (abyssal troughs with thick sediment cover) and III-2 (relatively flat areas covered by thick sediments) are characterized by low abundance of manganese nodules. This relationship between distribution of sonar facies and manganese nodule abundance implies that (1) the qualitative difference in acoustic reflectivity of long-range sidescan sonar with some ground truth data is useful for regional assessment of manganese nodule occurrence over wide areas in a reasonable time, and (2) seafloor topography and sediment thickness are important controlling factors for regional occurrences of manganese nodules.

Description: A semi-quantitative assessment is made of the animals observed in archived videotapes taken from the research submersible Jago, during diamond mining and exploratory surveys off the mouth of the Orange River on the west coast of southern Africa (28°15′S, 29°11′S) in November 1996. The seabed environment is described and nekton associations with substratum features are identified. The area is characterized by heterogeneity to its physical and biological struture. The variety of observed nekton is low, and communities are dominated by goby Sufflogobius bibarbatus, juvenile hake Merluccius spp. and cuttlefish Sepia spp. (on soft substrata), as well as false jacopever Sebastes capensis and kingklip Genypterus capensis (on rocky substrata).

Description: One kinds of glycolipid (SBI) have been isolated from the marine brown alga Sargassum hemiphyllum (Turn.) Ag. The structures of SBI have been determined as the sodium salt of 1-0-acyl-3-0-(6′-sulfo-α-D-quinovopyrannosyl) glycerol (acyl: tetradecanoyl, pentadecanoyl, 11-hexadecenoyl, hexadecanoyl, 10,13-octadecadienoyl, 9-octade cenoyl, 15-metylheptadecanoyl and 11-eicosenoyl 17:1.5:19:153:1:19:1:2) on the basis of chemical and spectral evidence and GC-MS analysis, respectively. Four constituents of the SBI were new compounds [the sodium salt of 1-0-(11″-hexadecenoyl)-3-0-(6′-sulfo-α-D-quinovopyrannosyl) glycerol, the sodium salt of 1-0-(10″,13″-octadecadienoyl)-3-0-(6′-sulfo-α-D-quinovopyrannosyl) glycerol, and the sodium salt of 1-0-(15″-metylhexadecenoyl)-3-0-(6′-sulfo-α-D-quinovopyrannosyl) glycerol, and the sodium salt of 1-0-(11″-eicosenoyl)-3-0-(6′-sulfo-α-D-quinovopyrannosyl) glycerol]. All compounds were isolated from marine brown alga for the first time.

Description: A new compound, jolynamine (1), was isolated from the marine brown alga Jolyna laminarioides collected from the coast of Karachi, Pakistan. In addition, four known compounds, namely saringosterol (2), loliolide (3), methyl-4-hydroxybenzoate (4) and propyl-4-hydroxybenzoate (5), were isolated for the first time from the marine brown alga Iyengaria stellata, and two known compounds, namely 3,4,5-trimethylaniline (6) and harmine (7), were isolated for the first time from the marine brown alga Melanothamnus afaqhusainii. Compound 6 is synthetically known but was isolated for the first time from a natural source. The structures of these compounds were elucidated with the help of powerful spectroscopic techniques. Furthermore, the methanolic extracts of both algae showed anti-microbial activities against various bacteria and fungi.

Description: In this paper, we empirically investigate the robustness of random forests for regression problems. We also investigate the performance of six variations of the original random forest method, all aimed at improving robustness. These variations are based on three main ideas: (1) robustify the aggregation method, (2) robustify the splitting criterion and (3) taking a robust transformation of the response. More precisely, with the first idea, we use the median (or weighted median), instead of the mean, to combine the predictions from the individual trees. With the second idea, we use least-absolute deviations from the median, instead of least-squares, as splitting criterion. With the third idea, we build the trees using the ranks of the response instead of the original values. The competing methods are compared via a simulation study with artificial data using two different types of contaminations and also with 13 real data sets. Our results show that all three ideas improve the robustness of the original random forest algorithm. However, a robust aggregation of the individual trees is generally more profitable than a robust splitting criterion.

Description: The annual winter sardine run along the South African east coast impacts the KwaZulu-Natal (KZN) coastal system in a variety of ways. These include ecological impacts, such as enrichment of a largely oligotrophic environment, competition between migrant sardine Sardinops sagax, other migrant and resident small pelagic fish species, and interactions with predators, as well as the socio-economic impacts of the sardine run on the local people. Enrichment of KZN coastal waters with organic nitrogen contained within the sardine is compared with alternative sources of nitrogen such as upwelling, river, sewage and stormwater runoff, and groundwater discharge. The sardine run appears to contribute most nitrogen to this system—96 000 t compared to 500–3 300 t for each of the other significant sources at trophic level 2, although upwelling estimates are extremely wide. Nonetheless, the majority of surviving sardine, their young and predators return southwards, suggesting that the nett export of nitrogen to KZN waters during the run is likely to be of a similar order of magnitude as that from other sources. Further, whereas the sardine supply of nitrogen is exclusively during winter, the bulk of the riverine input is in summer, thus ensuring that nitrogen supply in the region is maintained at fairly constant levels throughout the year. Competition for food between small pelagic fish is minimised by resource partitioning, but further dietary data are needed for resident species. Although interactions between sardine and top predators must exist, further studies are needed to confirm links between top predator life cycles and the sardine run. The estimated value of sardine as a tourist spectacle is compared to that from a seasonal beach-seine or boat-based purse-seine fishery for this species. Whereas the estimated value of the sardine as a tourist attraction appears substantially higher than could be derived from catching them, the small-scale beach-seine fishery itself draws tourists and also provides limited, seasonal employment opportunities.

Description: Our understanding of the fundamental regulatory roles that tyrosine phosphatases play within cells has advanced significantly in the last two decades. Out-dated ideas that tyrosine phosphatases acts solely as the “off” switch counterbalancing the action of tyrosine kinases has proved to be flawed. PTP1B is the most characterized of all the tyrosine phosphatases and it acts as a critical negative and positive regulator of numerous signaling cascades. PTP1B’s direct regulation of the insulin and the leptin receptors makes it an ideal therapeutic target for type II diabetes and obesity. Moreover, the last decade has also seen several reports establishing PTP1B as key player in cancer serving as both tumor suppressor and tumor promoter depending on the cellular context. Despite many key advances in these fields one largely ignored area is what role PTP1B may play in the modulation of immune signaling. The important recognition that PTP1B is a major negative regulator of Janus kinase – signal transducer and activator of transcription (JAK-STAT) signaling throughout evolution places it as a key link between metabolic diseases and inflammation, as well as a unique regulator between immune response and cancer. This review looks at the emergence of PTP1B through evolution, and then explore at the cell and systemic levels how it is controlled physiologically. The second half of the review will focus on the role(s) PTP1B can play in disease and in particular its involvement in metabolic syndromes and cancer. Finally we will briefly examine several novel directions in the development of PTP1B pharmacological inhibitors.