ALBERT

Beschreibung: Mixing processes of reduced hydrothermal fluids with oxygenated seawater and fluid-rock reactions contribute to the chemical signatures of diffuse venting and likely determine the geochemical constraints on microbial life. We examined the influence of fluid chemistry on microbial diversity and activity by sampling diffuse fluids emanating through mussel beds at two contrasting hydrothermal vents. The H(2) concentration was very low at the basalt-hosted Clueless site, and mixing models suggest O(2) availability throughout much of the habitat. In contrast, effluents from the ultramafic-hosted Quest site were considerably enriched in H(2) , while O(2) is likely limited to the mussel layer. Only two different hydrogenase genes were identified in clone libraries from the H(2) -poor Clueless fluids, but these fluids exhibited the highest H(2) uptake rates in H(2) -spiked incubations (oxic conditions, at 18 °C). In contrast, a phylogenetically diverse H(2) -oxidizing potential was associated with distinct thermal conditions in the H(2) -rich Quest fluids, but under oxic conditions, H(2) uptake rates were extremely low. Significant stimulation of CO(2) fixation rates by H(2) addition was solely illustrated in Quest incubations (P-value 〈0.02), but only in conjunction with anoxic conditions (at 18 °C). We conclude that the factors contributing toward differences in the diversity and activity of H(2) oxidizers at these sites include H(2) and O(2) availability.

Beschreibung: The macroalga Fucus vesiculosus carries a specific community of surface bacteria. To identify chemical compounds that possibly mediate abundance and community composition of algae-associated bacteria, we tested the effect of surface-available algal compounds on bacterial settlement and community composition under field conditions. Compounds on algal thalli were separated from the surface by extraction with organic solvents and investigated on growth inhibition and settlement of bacterial isolates. Based on in vitro data, partially purified extract fractions were then exposed to bacterial colonizers in vivo followed by bacterial enumeration and community analysis. The polar fraction of the algal surface extract revealed a significant profouling effect for Vibrionales, whereas the nonpolar fraction containing the xanthophyll pigment fucoxanthin and other unidentified nonpolar surface compounds revealed a significant 80% reduction of surface colonizing bacteria. The analysis of bacterial surface communities by 454 pyrosequencing demonstrated that the antifouling activity of nonpolar algal surface compounds was targeting the abundance of natural bacterial colonizers rather than the relative composition of bacterial members within the community. Moreover, the bacterial community composition on F.vesiculosus was markedly different from artificial control substrates and chemically manipulated experimental treatments, suggesting that other, nonextractable surface features and/or physical properties render algal-specific epiphytic bacterial communities.

Beschreibung: 18.10. - 25.10.2011

Beschreibung: Legionella pneumophila is naturally found in fresh water were the bacteria parasitize within protozoa. It also survives planctonically in water or biofilms. Upon aerosol formation via man-made water systems, L. pneumophila can enter the human lung and cause a severe form of pneumonia, called Legionnaires’ disease. The pathogenesis of Legionnaires’ disease is largely due to the ability of L. pneumophila to invade and grow within macrophages. An important characteristic of the intracellular survival strategy is the replication within the host vacuole that does not fuse with endosomes or lysosomes. In recent times a great number of bacterial virulence factors which affect growth of L. pneumophila in both macrophages and protozoa have been identified. The ongoing Legionella genome project and the use of genetically tractable surrogate hosts are expected to significantly contribute to the understanding of bacterium–host interactions and the regulation of virulence traits during the infection cycle. Since person-to-person transmission of legionellosis has never been observed, the measures for disease prevention have concentrated on eliminating the pathogen from water supplies. In this respect detection and analysis of Legionella in complex environmental consortia become increasingly important. With the availability of new molecular tools this area of applied research has gained new momentum.

Beschreibung: The GeoChip 4.2 gene array was employed to interrogate the microbial functional gene repertoire of sponges and seawater collected from the Red Sea and the Mediterranean. Complementary amplicon sequencing confirmed the microbial community composition characteristic of high microbial abundance (HMA) and low microbial abundance (LMA) sponges. By use of GeoChip, altogether 20 273 probes encoding for 627 functional genes and representing 16 gene categories were identified. Minimum curvilinear embedding analyses revealed a clear separation between the samples. The HMA/LMA dichotomy was stronger than any possible geographic pattern, which is shown here for the first time on the level of functional genes. However, upon inspection of individual genes, very few specific differences were discernible. Differences were related to microbial ammonia oxidation, ammonification, and archaeal autotrophic carbon fixation (higher gene abundance in sponges over seawater) as well as denitrification and radiation-stress-related genes (lower gene abundance in sponges over seawater). Except for few documented specific differences the functional gene repertoire between the different sources appeared largely similar. This study expands previous reports in that functional gene convergence is not only reported between HMA and LMA sponges but also between sponges and seawater.

Beschreibung: Following their transmission from the human to the mosquito with the bloodmeal, malaria parasites have to persevere in the mosquito midgut for approximately 1 d. During this period the parasites are highly vulnerable to factors of the mosquito midgut, including bacteria. We here aimed at determining the microbial diversity of gut bacteria of the Asian malaria vector Anophebs stephensi (Liston) during development and under different feeding regimes, including feeds on malaria parasite-infected blood. 16S rRNA and denaturing gradient gel electrophoresis analyses demonstrated an increasing reduction in the microbial diversity during mosquito development from egg to adult and identified the gram-negative bacterium Elizabethkingia meningoseptica King as the dominant species in the midgut of lab-reared male and female mosquitoes. E. meningoseptica is transmitted between generations and its predominance in the mosquito midgut was not altered by diet, when the gut microbiota was compared between sugar-fed and blood-fed female mosquitoes. Furthermore, feeds on blood infected with malaria parasites did not impact the presence of E. meningoseptica in the gut. Extracts from cultured E. meningoseptica were active against gram-positive and negative bacteria and yeast and against the blood and gametocyte transmission stages of the malaria parasite Plasmodium falciparum Welch. The antimicrobial and antiplasmodial activities of E. meningoseptica may account for its dominance in the midgut of the malaria vector.

Beschreibung: Dates of Cruise: 13. September to 19. September 2011 Projects: Student course in physical oceanography. Areas of Research: Physical oceanography Port call: Kiel – Warnemünde – Kiel

Beschreibung: The aim of this study was to examine sponge orange band (SOB) disease affecting the prominent Caribbean sponge Xestospongia muta. Scanning and transmission electron microscopy revealed that SOB is accompanied by the massive destruction of the pinacoderm. Chlorophyll a content and the main secondary metabolites, tetrahydrofurans, characteristic of X. muta, were significantly lower in bleached than in healthy tissues. Denaturing gradient gel electrophoresis using cyanobacteria-specific 16S rRNA gene primers revealed a distinct shift from the Synechococcus/Prochlorococcus clade of sponge symbionts towards several clades of unspecific cyanobacteria, including lineages associated with coral disease (i.e. Leptolyngbya sp.). Underwater infection experiments were conducted by transplanting bleached cores into healthy individuals, but revealed no signs of SOB development. This study provided no evidence for the involvement of a specific microbial pathogen as an etiologic agent of disease; hence, the cause of SOB disease in X. muta remains unidentified.

Beschreibung: Ferredoxins are electron shuttles harbouring iron–sulfur clusters that connect multiple oxido-reductive pathways in organisms displaying different lifestyles. Some prokaryotes and algae express an isofunctional electron carrier, flavodoxin, which contains flavin mononucleotide as cofactor. Both proteins evolved in the anaerobic environment preceding the appearance of oxygenic photosynthesis. The advent of an oxygen-rich atmosphere proved detrimental to ferredoxin owing to iron limitation and oxidative damage to the iron–sulfur cluster, and many microorganisms induced flavodoxin expression to replace ferredoxin under stress conditions. Paradoxically, ferredoxin was maintained throughout the tree of life, whereas flavodoxin is absent from plants and animals. Of note is that flavodoxin expression in transgenic plants results in increased tolerance to multiple stresses and iron deficit, through mechanisms similar to those operating in microorganisms. Then, the question remains open as to why a trait that still confers plants such obvious adaptive benefits was not retained. We compare herein the properties of ferredoxin and flavodoxin, and their contrasting modes of expression in response to different environmental stimuli. Phylogenetic analyses suggest that the flavodoxin gene was already absent in the algal lineages immediately preceding land plants. Geographical distribution of phototrophs shows a bias against flavodoxin-containing organisms in iron-rich coastal/freshwater habitats. Based on these observations, we propose that plants evolved from freshwater macroalgae that already lacked flavodoxin because they thrived in an iron-rich habitat with no need to back up ferredoxin functions and therefore no selective pressure to keep the flavodoxin gene. Conversely, ferredoxin retention in the plant lineage is probably related to its higher efficiency as an electron carrier, compared with flavodoxin. Several lines of evidence supporting these contentions are presented and discussed.

Beschreibung: RV Sonne cruise 206 started on the 31st of May in Caldera (Costa Rica) and ended there on the 18th of June 2010. An international group of 23 scientists and 2 guests from Costa Rica set out to perform geophysical, biogeochemical, and sedimentological investigations at and around a number of submarine cold seeps that are ubiquitous along this convergent continental margin. The cruise was predominantly conducted as a pre-site survey for IODP (proposal 633Full-2; Costa Rica Mounds) in order to fill existing gaps in the seismic record at two of the proposed drill locations. A 2D seismic survey was conducted running over the large-scale seamount subduction slide “Jaco Scarp” yielding a highly improved image of the structural pattern within the upper 1000 m of the sedimentary sequence. In addition, high-resolution 3D seismic data, roughly covering the uppermost 300 m, could be obtained in the Mound 11/12 area, which will allow for a detailed analysis of the internal structure of these mud volcano-like features and help to understand the complex relation to the upward directed material flow. Moreover, a total number of 36 sediment cores (TV-guided multicorer, gravity corer) were successfully retrieved from active cold seeps and submarine slides and a large number of sub-samples were taken for subsequent geochemical and microbiological analyses. Overall, all major tasks of this cruise could successfully be fulfilled and the results will help to further our understanding of mechanisms controlling fluid flow in the deep subsurface of active continental margins.

Beschreibung: Dates of Cruise: 11.10. to 14.10.2004 Projects: BASEWECS and Student course in physical oceanography; Port Call: Warnemünde (11.10.)

Beschreibung: Dates of Cruise: 29.06. to 02.07.2004 Projects: BASEWECS and Student course in physical oceanography Port Calls: Warnemünde (29.06.; 01.07) and Sassnitz (30.06.)

Beschreibung: Seismic oceanography is a new field in marine research combining expertise of proven geophysical methods with classical physical oceanography to get more insight into physical processes in the water column. An interdisciplinary working group at IFM-GEOMAR has sucessfully used the in-house expertise for deep ocean investigations.

Beschreibung: The Kieler Wirkstoff-Zentrum KiWiZ (Centre for Marine Natural Products) at IFM-GEOMAR is investigating opportunities to use substances from microbial communties for medical and other applications. The new field of Marine Biotechnology is an emering area in Marine Sciences.

Beschreibung: Tens of thousands of submarine volcanoes, so-called seamounts, with heights of more than a few hundred meters, exist in the world ocean. The Cape Verde archipelago is of volcanic origin and includes several prominent seamounts, which can give important information on the origin of submarine intraplate volcanism.

Beschreibung: Globally averaged sea level has risen by just under 10 cm during the last 50 years as a consequence of global warming. The rise, however, is not uniform, neither in time nor in space. Natural climate fluctuations and associated changes in the ocean currents have contributed to the inhomogeneity and is an important factor which will determine the pattern of future sea level rise. While research in the past years has focused on the global-mean trend and its attribution to the melting of glaciers and the thermal expansion of sea water under global warming, attention is shifting to the geographical pattern of sea level change. This is essential for coastal impact assessments, but has not been practical yet because ocean projections from current climate models widely diverge. The improvement of regional sea level prediction requires a better understanding of the underlying dynamical causes.

Beschreibung: Precise isotope analysis has developed to one of the most important tool in marine geochemistry. Accurate isotope fractionation is used for climate reconstructions for millions years back into the Earth’s history.

Beschreibung: Never in the last 2,000 years was the Atlantic Water entering the Arctic in the Fram Strait between Greenland and Svalbard as warm as today. This was revealed by a study of marine sediments from the western Svalbard continental margin which was led by researchers from IFM-GEOMAR.

Beschreibung: A PCR protocol for the detection of sulfur-oxidizing bacteria based on soxB genes that are essential for thiosulfate oxidation by sulfur-oxidizing bacteria of various phylogenetic groups which use the 'Paracoccus sulfur oxidation' pathway was developed. Five degenerate primers were used to specifically amplify fragments of soxB genes from different sulfur-oxidizing bacteria previously shown to oxidize thiosulfate. The PCR yielded a soxB fragment of approximately 1000 bp from most of the bacteria. Amino acid and nucleotide sequences of soxB from reference strains as well as from new isolates and environmental DNA from a hydrothermal vent habitat in the North Fiji Basin were compared and used to infer relationships of soxB between sulfur-oxidizing bacteria belonging to various 16S rDNA-based phylogenetic groups. Major phylogenetic lines derived from 16S rDNA were confirmed by soxB phylogeny. Thiosulfate-oxidizing green sulfur bacteria formed a coherent group by their soxB sequences. Likewise, clearly separated branches demonstrated the distant relationship of representatives of alpha-, beta-, and gamma-Proteobacteria including representative species of the former genus Thiobacillus (now Halothiobacillus - gamma-Proteobacteria, Thiobacillus - beta-Proteobacteria and Starkeya - alpha-Proteobacteria). This general picture emerged although apparent evidence for lateral transfer of the soxB gene is indicated and comparison of soxB phylogeny and 16S rDNA phylogeny points to the significance of this gene transfer in hydrothermal vent bacterial communities of the North Fiji Basin.

Beschreibung: Phototrophic bacteria are important primary producers of salt lakes in the Salar de Atacama and at times form visible mass developments within and on top of the lake sediments. The communities of phototrophic bacteria from two of these lakes were characterized by molecular genetic approaches using key genes for the biosynthesis of the photosynthetic apparatus in phototrophic purple bacteria (pufLM) and in green sulfur bacteria (fmoA). Terminal restriction fragment length polymorphism of the pufLM genes indicated high variability of the community composition between the two lakes and subsamples thereof. The communities were characterized by the dominance of a novel, so far undescribed lineage of pufLM containing bacteria and the presence of representatives related to known halophilic Chromatiaceae and Ectothiorhodospiraceae. In addition, the presence of BChl b-containing anoxygenic phototrophic bacteria and of aerobic anoxygenic bacteria was indicated. Green sulfur bacteria were not detected in the environmental samples, although a bacterium related to Prosthecochloris indicum was identified in an enrichment culture. This is the first comprehensive description of phototrophic bacterial communities in a salt lake of South America made possible only due to the application of the functional pufLM genes.

Beschreibung: The research project SO208 PLUMEFLUX (Propagation of Galápagos Plume Material in the Equatorial East Pacific) is funded by the German Ministry of Education and Research (BMBF) and focusses on seamounts on the Cocos Plate, formed at the East Pacific Rise but presently located off the coast of NW Costa Rica and Nicaragua (Leg 1), and on the Cocos-Nazca-Spreading Center(CNS; Leg 2). The studies conducted on cruise SO208 included multi-beam mapping, sediment echo sounding, hard rock sampling, as well as sedimentological and biological sampling. The major geological targets of Leg 1 were (1) to evaluate if the seamounts off northern Costa Rica and Nicaragua were formed by the Galápagos hotspot and thus to constrain the extent of influence by the Galápagos plume on the upper asthenosphere and lithosphere and (2) to constrain better the material input into the Central American subduction zone. Leg 2 aimed to sample profiles of the seafloor perpendicular to the CNS using the mobile drill Rockdrill 2 of the British Geological Survey, in order to reconstruct plume-ridge interaction in the past to complement morphological, petrological and geochemical studies carried out on previous cruises along the ridge axis (e.g. SO158). The integration of the results with existing data should contribute towards a better understanding of transport processes of plume material in the upper mantle and of the geodynamic evolution of the central East Pacific. The sedimentological studies carried out on both Legs of SO208 focussed on radiolarian assemblages. SO208 startet in Caldera/Costa Rica on July 15th, 2010, and ended in Guayaquil/Ecuador on August 29th, 2010. During Leg 1 nineteen seamounts on the Cocos Plate have been mapped and sampled. Within only 12 days at sea, a total of 41 stations(28 dredges, 2 TV grabs, and 11 multicorers) were carried out. Of these deployments, 23 recovered magmatic rocks, 15 volcaniclastics, and 13 Mn-Fe oxides. The samples comprise mainly pillow and sheet lavas, often with fresh glassy margins, and a wide spectrum of volcaniclastic rocks. Notably, we found several indications for explosive volcanic activity at water depths 〉 3,000 m. Due to a series of unfortunate circumstances beyond our control, the planned Rockdrill 2 deployment on Leg 2 had to be cancelled. Instead we mapped and collected samples via dredging from 60 localities along five profiles perpendicular to CNS. A total of 83 stations (76 dredges, 3 TVgrabs, and 4 multicorers) were carried out. Of these deployments, 59 recovered magmatic rocks, 3 volcaniclastics, 51 volcanic glass, and 6 Mn-Fe oxides, making this a successful cruise despite the Rockdrill problem. The first and most detailed profile (with 23 sampled localities) extended from the ridge axis to the north at ~92°W. The morphology shows alternating regions of abyssal ridges and valleys (possibly reflecting less plume input into the ridge) and thicker elevated bands commonly containing seamounts, some of which are tectonically deformed (possibly reflecting axial ridge type morphology and thus greater plume input into the ridge). The second profile was carried out at the shallowest part of the ridge axis, closest to the hotspot, just to the east of the 91° Transform Fault. The third profile extended 30 km north of the ridge (at ~89°30´W), where a formerly on-axis seamount has been split in half. We want to evaluate how far in the past the unique enriched geochemical anomaly associated with the seamount persisted in the past. Thirteen sites were successfully sampled along a fourth profile at 89°10’W, extending 35 km north and 35 km south of the CNS into crust up to 500,000 yrs old. This site was selected, because a major depleted geochemical anomaly exists at that location. A final short profile was carried out to the north and south and east (on the ridge axis) of a lava plateau at 88°20’W that represents an enriched anomaly along the ridge axis. The morphology of the area studied on Leg 2 strongly suggests that the intensity of interaction of the plume with the ridge has varied considerably over the last several hundred thousand years along the entire part of the ridge that we studied. Geochemical data should allow us to constrain better variations in plume-ridge interaction through time. Biological material was obtained successfully as macrofauna and as sediment samples containing meiofaunal organisms with the help of a geological chain bag dredge, sediment traps, a multicorer and a TV-grab. Macrofaunal organisms were recovered at 93 out of 124 stations, 37 stations revealed sediment samples. During the cruise, a total of 8,598 meiofaunal organisms were centrifuged out of about 45 kg of sediment and sorted to animal group. Foraminifera and Nematoda dominated the meiofauna, followed by Copepoda and at a lower abundance by Tardigrada, Kinorhyncha and Loricifera. We also discovered a highly diverse benthic macrofauna, mainly represented by Porifera, Bryozoa, Annelida, Brachiopoda and even a few monoplacophoran molluscs.

Beschreibung: The overall objective of the subproject A3 of SFB 460* (1996 - 2003) was to observe the water mass transformation in the eastern basin of the subpolar gyre with direct methods including RAFOS floats. Our floats populated the 1500 m depth range of the Iceland Basin occupied by low salinity Labrador Sea Water and higher salinity Overflow Water. The first water mass reaches the Basin through the Charlie-Gibbs Fracture Zone. Its source region is in the Labrador Sea where it is generated on a yearly basis by deep reaching winter time convection. The second intermediate water mass of the eastern basin enters the region as Iceland Scotland Overflow Water with its perpetual source in the Norwegian Seas. At the southeastern margin of our region of interest remainders of Mediterranean Water mix with the other two characteristic water masses. A smaller number of floats was deployed in the lower deep water of the Iceland Basin at nominally 2600m depth. Data for the presented gallery of intermediate float trajectories were collected between summer 1997 and summer 2003. We have tracked 57 RAFOS floats. These neutrally buoyant in-situ drifters were launched during six research cruises. Their average underwater missions exceeded one year. The Lagrangian experiment lasted until 2003 when all four sound sources were completely recovered. The data set comprises also trajectories from float parks. These ensembles of floats enable repeated Lagrangian time series with identical initial conditions allowing estimates of the representativeness of individual trajectories. *SFB (Sonderforschungsbereich) stands for an accelerated research initiative comprising intensive observations and modelling efforts of fluctuations in the thermohaline circulation in the subpolar gyre of the North Atlantic.

Beschreibung: Satellite images document a decrease in Arctic ice cover in summer by 40% during the past 30 years. However, responsible processes and possible consequences of this decrease are little understood. The joint Russian-German project „Laptev Sea Polynya“ is coordinated by IFM-GEOMAR and aims to ascertain the causes and consequences of climate change and its essential mechanisms in the Arctic. Now the project scientists revealed a changed water mass distribution in the Siberian Laptev Sea which might be of consequence for ice formation in the whole Arctic.

Beschreibung: El Niño-Southern Oscillation (ENSO) is the strongest natural climate phenomenon on timescales from months to a few years. Sucessfully modelling and subsequently forecasting ENSO is still a challenge although significant progess has been made during the past decades. IFM-GEOMAR scientists developed a new model which is currently being tested for ENSO applications.

Beschreibung: Marine scientists studying the seafloor always have to deal with the fact that the research vessel they are sailing with will be separated from the object they wish to study by several kilometers of water. Technological developments are helping IFM-GEOMAR scientists to overcome this problem and producing astounding results.

Beschreibung: Tectonic stresses lead to the deformation of the Earth’s crust and upper mantle. They are the driving force behind plate motion and mountain building, and they control earthquake distribution and intensity. These stresses can be measured from deformation microstructures of previously deeply buried rocks using paleo-piezometers. A new assessment of microstructural data from naturally deformed quartz-bearing rocks indicates that the mechanism of recrystallization fundamentally affects the piezometers. Our findings point out major inaccuracies of stress estimates published in the last 40 years, and it prepares the field for a new piezometer generation that will provide a significantly improved assessment of the stress states in lithospheric plates and plate boundaries.

Beschreibung: The concentration of CO2 in global surface ocean waters is increasing due to rising atmospheric CO2 emissions, resulting in lower pH and a lower saturation state of carbonate ions. Such changes in seawater chemistry are expected to impact calcification in calcifying marine organisms. However, other physiological processes related to calcification might also be affected, including enzyme activity. In a mesocosm experiment, macroalgal communities were exposed to three CO2 concentrations (380, 665, and 1486 µatm) to determine how the activity of two enzymes related to inorganic carbon uptake and nutrient assimilation in Corallina officinalis, an abundant calcifying rhodophyte, will be affected by elevated CO2 concentrations. The activity of external carbonic anhydrase, an important enzyme functioning in macroalgal carbon-concentrating mechanisms, was inversely related to CO2 concentration after long-term exposure (12 weeks). Nitrate reductase, the enzyme responsible for reduction of nitrate to nitrite, was stimulated by CO2 and was highest in algae grown at 665 µatm CO2. Nitrate and phosphate uptake rates were inversely related to CO2, while ammonium uptake was unaffected, and the percentage of inorganic carbon in the algal skeleton decreased with increasing CO2. The results indicate that the processes of inorganic carbon and nutrient uptake and assimilation are affected by elevated CO2 due to changes in enzyme activity, which change the energy balance and physiological status of C. officinalis, therefore affecting its competitive interactions with other macroalgae. The ecological implications of the physiological changes in C. officinalis in response to elevated CO2 are discussed.

Beschreibung: The sheer scale of the metagenomic and metatranscriptomic datasets that are now available warrants the development of automated protocols for organizing, annotating and comparing the samples in terms of their metabolic profiles. We describe a user-friendly java program FROMP (Fragment Recruitment on Metabolic Pathways) for mapping and visualizing enzyme annotations onto the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways or custom-made pathways and comparing the samples in terms of their Pathway Completeness Scores,their relative Activity Scores or enzyme enrichment odds ratios. This program along with our fully-configurable PERL based annotation organization pipeline Meta2Pro (METAbolic PROfiling of META-omic data) offers a quick and accurate standalone solution for metabolic profiling of environmental samples or cultures from different treatments. Apart from pictorial comparisons, FROMP can also generate score matrices for multiple meta-omics samples which can be used directly by other statistical programs

Beschreibung: Effects of epibiotic bacteria associated with macroalgae on barnacle larval attachment were investigated. Eight bacterial isolates obtained from samples of three macroalga species were cultured as monospecies bacterial films and tested for their activity against barnacle (Amphibalanus improvisus) attachment in field experiments (Western Baltic Sea). Furthermore, natural biofilm communities associated with the surface of the local brown alga, Fucus vesiculosus, which were exposed to different temperatures (5, 15 and 20 °C), were harvested and subsequently tested. Generally, monospecies bacterial biofilms, as well as natural microbial assemblages, inhibited barnacle attachment by 20-67%. denaturing gradient gel electrophoresis fingerprints showed that temperature treatment shifted the bacterial community composition and weakened the repellent effects at 20 °C. Repellent effects were absent when settlement pressure of cyprids was high. Nonviable bacteria tended to repel cyprids when compared to the unfilmed surfaces. We conclude that biofilms can have a repellent effect benefiting the host by preventing heavy fouling on its surface. However, severe settlement pressure, as well as stressful temperature, may reduce the protective effects of the alga's biofilm. Our results add to the notion that the performance of F. vesiculosus may be reduced by multiple stressors in the course of global warming.

Beschreibung: Changing environmental conditions, worldwide transport by commerical ships provide the basis for the increasing rates of marine invasions with substantial impact of marine ecosystems. Here we focus on the comb jelly Mnemiopsis leidyi one of the most prominent marine invaders. worldwide.

Beschreibung: Climate warming will affect the phytoplankton spring bloom. Experiments conducted at IFM-GEOMAR indicate, that bloom biomass of phytoplankton will become less, average cell size will become smaller, the biological carbon pump will be weakened and the timing of the bloom will be advanced a bit in a warming climate.

Beschreibung: The passive NW-African continental margin is characterized by several very large but infrequent landslides. Most of these landslides occurred during periods of low or rising sea level. Hence, the probability of future large-scale slope failures during the current sea level high stand is generally considered to be low. This interpretation is challenged by new observations during a recent research cruise to the Sahara Slide.

Beschreibung: The cold waters of the polar seas are naturally low in carbonate saturation. As uptake of anthropogenic CO2 continues to acidify the oceans worldwide, the Arctic Ocean will be the first to pass the chemical threshold where surface seawater becomes undersaturated, i.e. corrosive for unprotected shells and skeletons of calcifying organisms. While the overall impacts of ocean acidification on the Arctic ecosystems are still unknown, the predicted changes in seawater chemistry are expected to make it increasingly difficult for calcareous organisms to inhabit these regions.

Beschreibung: The Collaborative Research Centre 574: Fluids and Volatiles in Subduction Zones has the overarching goal to understand the role and fate of volatiles and fluids in the entire subduction system. The SONNE cruise SO-210 was the last major expedition of the 12-year programme.

Beschreibung: Human-induced climate change is causing a warming of the surface ocean. Due to widely differing temperature sensitivities of key biological processes, this may have profound implications for marine food web interactions and the biogeochemical cycling of key elements such as carbon. Using a novel indoor-mesocosm approach, we show that rising sea surface temperature shifts the balance between photosynthetic production and respiratory consumption of organic carbon in a plankton community. This may weaken the ocean’s capacity to sequester atmospheric CO2, hence providing a positive feedback to anthropogenic climate change.

Beschreibung: Carbon capture and storage (CCS), both on- and offshore, is expected to be an important technique to mitigate anthropogenic effects on global climate by isolating man-made carbon dioxide (CO2) in deep geological formations. In marine environments, however, the potential impacts of CO2 leakage, appropriate detection methods, and risk and pathways of atmospheric emissions are poorly defined. The natural CO2 gas seeps that occur in the relatively shallow waters off the coast of Panarea Island (Aeolian Islands, Italy) can be studied as a large-scale, real-world analogue of what might occur at a leaking offshore CCS site and what tools can be used to study it. The oceanographic survey PaCO2 was performed aboard R/V Urania from 27 July – 01 August 2011 (Naples – Naples). The project’s ship-time was funded by Eurofleets, with work being performed as a sub-project of the Seventh Framework Programme projects “ECO2” and “RISCS”, which provided subsidiary funding. Large amounts of data and samples were collected during the cruise which will be interpreted in the coming months, with preliminary results detailed here. Of particular importance was the discovery of much larger areas showing gas seepage than previously reported. Interdisciplinary measurements were performed at the Panarea seepage site. The international team of scientists onboard R/V Urania performed complementary sampling and measurements for biological, chemical, and physical parameters throughout the area. Together with the dedication of R/V Urania’s Captain and crew, and the eagerness and cooperation of the scientific crew, we were able to obtain excellent scientific results during this six-day cruise.

Beschreibung: In this study, the impact of oceanic data assimilation on ENSO simulations and predictions is investigated. The authors’ main objective is to compare the impact of the assimilation of sea level observations and three-dimensional temperature measurements relative to each other. Three experiments were performed. In a control run the ocean model was forced with observed winds only, and in two assimilation runs three-dimensional temperatures and sea levels were assimilated one by one. The root-mean-square differences between the model solution and observations were computed and heat content anomalies of the upper 275 m compared to each other. Three ensembles of ENSO forecasts were performed additionally to investigate the impact of data assimilation on ENSO predictions. In a control ensemble a hybrid coupled ocean–atmosphere model was initialized with observed winds only, while either three-dimensional temperatures or sea level data were assimilated during the initialization phase in two additional forecast ensembles. The predicted sea surface temperature anomalies were averaged over the eastern equatorial Pacific and compared to observations. Two different objective skill measures were computed to evaluate the impact of data assimilation on ENSO forecasts. The authors’ experiments indicate that sea level observations contain useful information and that this information can be inserted successfully into an oceanic general circulation model. It is inferred from the forecast ensembles that the benefit of sea level and temperature assimilation is comparable. However, the positive impact of sea level assimilation could be shown more clearly when the forecasted temperature differences rather than the temperature anomalies themselves were compared with observations.

Beschreibung: Within the Jeddah Transect Project, a multidisciplinary marine sampling program has been performed on RV Poseidon (P408 cruise) offshore Saudi-Arabia (Red Sea). The sampling program involved three projects (http://www.ifm-geomar.de/index.php?id=jeddahtransectdisc&L=1), covering aspects of marine petrology, biogeochemistry, oceanography and biology.

Beschreibung: Shallow coastal waters, where phototrophic purple sulfur bacteria (PSB) regularly form massive blooms, are subjected to massive diurnal and event-driven changes of physicochemical conditions including temperature and salinity. To analyze the ability of PSB to cope with these environmental factors and to compete in complex communities we have studied changes of the environmental community of PSB of a Baltic Sea lagoon under experimental enrichment conditions with controlled variation of temperature and NaCl concentration. For the first time, changes within a community of PSB were specifically analyzed using the photosynthetic reaction center genes pufL and M by RFLP and cloning experiments. The most abundant PSB phylotypes in the habitat were found along the NaCl gradient from freshwater conditions up to 7.5% NaCl. They were accompanied by smaller numbers of purple nonsulfur bacteria and aerobic anoxygenic phototrophic bacteria. Major components of the PSB community of the brackish lagoon were affiliated to PSB genera and species known as marine, halophilic or salt-tolerant, including species of Marichromatium, Halochromatium, Thiorhodococcus, Allochromatium, Thiocapsa, Thiorhodovibrio, and Thiohalocapsa. A dramatic shift occurred at elevated temperatures of 41 and 44°C when Marichromatium gracile became most prominent which was not detected at lower temperatures.

Beschreibung: The DRIVE (Diurnal and RegIonal Variability of Halogen Emissions) campaign to the eastern tropical North Atlantic Ocean and the upwelling off Mauritania (NW Africa) was funded by the BMBF as part of the German SOLAS project SOPRAN II (Surface Ocean Processes in the Anthropocene; www.sopran.pangaea.de): The second leg of the 399th cruise of R/V Poseidon (P399/2) took place from 31 May to 17 June 2010 (Las Palmas-Mindelo (Cape Verde Islands) – Mauritanian upwelling – Las Palmas). It was followed by the transit leg 3 (P399/3) which took place from 18 June to 24 June 2010 (Las Palmas – Vigo (Spain)) with only one stop at ESTOC. Ten scientists from IFM-GEOMAR (Kiel), IfAM (U Kiel), IfBM (U Hamburg) and IUP (U Heidelberg) representing various SOPRAN II subprojects took part in the cruise which was the sixth of a series of German SOLAS cruises to the tropical North Atlantic Ocean. The major objective of P399/2 was to investigate the regional and diurnal atmospheric and oceanic variations of halogenated compounds in the eastern tropical North Atlantic Ocean with a special focus on the Mauritanian upwelling. The main working packages of P399/2 and P399/3 included measurements of - Atmospheric BrO and IO - Atmospheric halocarbons - Other atmospheric trace gases such as ozone, methane etc. - Aerosol composition - Vertical structure of the atmosphere - Dissolved halocarbons, nitrous oxide and carbon dioxide - CTD, dissolved nutrients, O2, and chlorophyll - Microstructure of the upper water column Besides an extensive underway measurement program of dissolved (halocarbons, N2O, CO2) and atmospheric (BrO, halocarbons, other trace gases, aerosol) compounds, six 24h stations were performed and 23 regular CTD stations with depth profiles covering the entire water column were occupied.

Beschreibung: The seasonal cycle over the tropical Pacific simulated by 11 coupled ocean–atmosphere general circulation models (GCMs) is examined. Each model consists of a high-resolution ocean GCM of either the tropical Pacific or near-global means coupled to a moderate- or high-resolution atmospheric GCM, without the use of flux correction. The seasonal behavior of sea surface temperature (SST) and eastern Pacific rainfall is presented for each model. The results show that current state-of-the-art coupled GCMs share important successes and troublesome systematic errors. All 11 models are able to simulate the mean zonal gradient in SST at the equator over the central Pacific. The simulated equatorial cold tongue generally tends to be too strong, too narrow, and extend too far west. SSTs are generally too warm in a broad region west of Peru and in a band near 10°S. This is accompanied in some models by a double intertropical convergence zone (ITCZ) straddling the equator over the eastern Pacific, and in others by an ITCZ that migrates across the equator with the seasons; neither behavior is realistic. There is considerable spread in the simulated seasonal cycles of equatorial SST in the eastern Pacific. Some simulations do capture the annual harmonic quite realistically, although the seasonal cold tongue tends to appear prematurely. Others overestimate the amplitude of the semiannual harmonic. Nonetheless, the results constitute a marked improvement over the simulations of only a few years ago when serious climate drift was still widespread and simulated zonal gradients of SST along the equator were often very weak.

Beschreibung: Analyses of clone libraries from water and sediments of different sites from Salar de Huasco, a high-altitude athalassohaline wetland in the Chilean Altiplano, revealed the presence of five unique clusters of uncultured Archaea that have not been previously reported or specifically assigned. These sequences were distantly related (83–96% sequence identity) to a limited number of other clone sequences and revealed no identity to cultured Archaea. The abundance of Archaea and Bacteria was estimated using qPCR and community composition was examined through the construction of clone libraries of archaeal 16S rRNA gene. Archaea were found to be dominant over Bacteria in sediments from two saline sites (sites H4: 6.31 × 104 and site H6: 1.37 × 104 μS cm−1) and in one of the water samples (freshwater from site H0: 607 μS cm−1). Euryarchaeotal sequences were more abundant than crenarchaeotal sequences. Many of the clone sequences (52%) were similar to uncultured archaeal groups found in marine ecosystems having identity values between 99% and 97%. A major fraction of the sequences (40%) were members of Methanobacteria, while others were included in the Marine Benthic Groups B and D, the Miscellaneous Crenarchaeotic Group, the Terrestrial Miscellaneous Euryarchaeotal Group, Marine Group I and Halobacteria. The presence of uncultured archaeal groups in Salar de Huasco extends their known distribution in inland waters, providing new clues about their possible function in the environment.

Beschreibung: Life at deep-sea hydrothermal vents depends on chemolithoautotrophic microorganisms as primary producers mediating the transfer of energy from hydrothermal fluids to higher trophic levels. A comprehensive molecular survey was performed with microbial communities in a mussel patch at the Irina II site of the Logatchev hydrothermal field by combining the analysis of 16S rRNA gene sequences with studies of functional key genes involved in biochemical pathways of sulfur oxidation–reduction (soxB, aprA) and autotrophic carbon fixation (aclB, cbbM, cbbL). Most significantly, major groups of chemoautotrophic sulfur oxidizers in the diffuse fluids differed in their biosynthetic pathways of both carbon fixation and sulfur oxidation. One important component of the community, the Epsilonproteobacteria, has the potential to grow chemoautotrophically by means of the reductive tricarboxylic acid cycle and to gain energy through the oxidation of reduced sulfur compounds using the Sox pathway. The majority of soxB and all retrieved aclB gene sequences were assigned to this group. Another important group in this habitat, the Gammaproteobacteria, may use the adenosine 5'-phosphosulfate pathway and the Calvin–Benson–Bassham cycle, deduced from the presence of aprA and cbbM genes. Hence, two important groups of primary producers at the investigated site might use different pathways for sulfur oxidation and carbon fixation.

Beschreibung: 14.10.-16.10.2002

Beschreibung: 19.02.-21.02.2003

Beschreibung: Cruise SO210 with RV SONNE to the active continental margin off Chile was conducted by shiptime exchange with RV METEOR. Funds for mobilizing the research team were provided by the German Science Foundation (DFG) in conjunction with the Collaborative Research Centre (SFB) 574 of the University of Kiel. In the first years, the SFB 574 investigated the pathways and fluxes of volatiles through the erosive subduction zone off Central America. For comparison, the studies were extended to the accretionary margin off Central Chile. Cruise SO210 is the last cruise conducted in the framework of SFB 574 and based on investigations of previous SFB-cruises on the RVs VIDAL GORMAZ and JAMES COOK. The first leg of cruise SO210 was dedicated to long gravity coring for volcanic ash layers from the erruptive Southern Volcanic Zone (SVZ) of the Andes that were either deposited as fallouts onto the incoming Nazca Plate or transported down the slope and across the Chile Trench. Eight gravity cores of 12 m length were retrieved seaward of the Chile Channel on the outer rise of the Nazca Plate. The second goal for coring was the description and dating of previously mapped submarine landslides as well as retrieval of slide-related material for geo-technical experiments. As the deployment frame for long coring had to be removed on the second leg we continued coring for mass-wasting and geochemistry with short cores. Ten gravity cores of 3 or 6 m barrel length were retrieved upslope of slides, the glide plane and redeposited material downslope of the slide evacuation area. This sampling activity was supported by detailed acoustic surveys with Parasound and multibeam to remap critical areas for mass wasting in search for events, e.g. triggered by the recent Mw 8.8 Maule Earthquake, such as flanks of submarine canyons or previously detected submarine slides and to fill data gaps in the existing bathymetric data. The major activity of the entire cruise was dedicated to the search and detailed sampling of manifestations of fluid discharge activity on the Chilean forearc. A total of 11 deployments with the video sled OFOS and 12 dives by the ROV KIEL 6000 were conducted for ground-truthing of information which indicated possible seep activity and has been obtained during previous cruises to the Chilean forearc. In five working areas we found manifestations of fluid discharge. In these areas the survey was followed by an intense sampling of bottom water, sediments, carbonates, mega and meiofauna and the deployment of instrumentation on the seafloor. The goal of these deployments was to measure in situ seabed methane emission rates and associated fluxes of sulfide and major electron acceptors such as oxygen at seep sites along the Chilean margin and to understand its controls. This was accompanied by CTD casts to trace oxygen and the fate of methane discharge in the water column. Sediment cores obtained by multicorer or ROV were used for the geochemical characterization of the pore water and microbiological studies which include turnover rate measurements, molecular studies, flow through experiments and sampling of active sediments. Authigenic carbonates obtained by TV-Grab or ROV were sampled for fauna, biomarker studies and investigations to reconstruct the growth structures, calcification processes and fluid-pathway systematic. The sampling of sediments and carbonates recovered a unique fauna with 79 different taxa, several of them appear to be species new to science.

Beschreibung: The activity of the human armpit microbiota triggers the formation of body odor. We used differential 16S rRNA gene (rDNA)- and rRNA-based terminal-restriction fragment length polymorphism fingerprinting in combination with cloning and sequencing to identify active members of the human armpit microbiota. DNA and RNA were isolated from skin scrub samples taken from both armpits of 10 preconditioned, healthy males. The fingerprint profiles indicated pronounced similarities between the armpit microbiota in the right and the left axillae of an individual test person, but larger differences between the axilla microbiota of different individuals. Using 16S rDNA and rRNA sequence data, the majority of peaks in the armpit profiles were assigned to bacteria affiliated with well-known genera of skin bacteria. The relative abundances of all groups were similar among the rDNA and rRNA samples, suggesting that all groups of armpit bacteria were active. Surprisingly, the relative abundance of sequences affiliated with Peptoniphilus sp. was by far and with statistical significance the highest in the rRNA samples of the right armpits. Thus, bacteria affiliated with Peptoniphilus sp. might have been particularly active in the right axillae of the test persons, possibly owing to the handedness of the test persons, which might cause different environmental conditions in the right axillae.

Beschreibung: We investigated the gelatinous carnivore zooplankton community in the coastal waters of northeast Taiwan during the period 20072010. The community assemblage was composed of 45 species, of which only 14 appeared recurrent in Taiwanese waters. Although there was no clear seasonality, higher richness and abundances occurred in spring and autumn. Examination of potential physical drivers of coastal biomass accumulation did not show any link with water mass transport; instead, peak events were associated with typhoon disturbances, suggesting a potential resource pulse effect.

Beschreibung: Helena, Tim, Melanie und Tim, die sich in ihren Sommerferien langweilen, stolpern über einen Artikel über Sauerstoffminimumzonen. Sie beschließen, verschiedene Meereslebewesen vor Ort zu befragen, was es damit auf sich hat. Bei ihrer Recherche tauchen sie von der Meeresoberfläche bis 500 Meter unter dem Meer ...

Beschreibung: El Niño–Southern Oscillation (ENSO) in the Pacific and the analogous Atlantic Niño mode are generated by processes involving coupled ocean–atmosphere interactions known as the Bjerknes feedback. It has been argued that the Atlantic Niño mode is more strongly damped than ENSO, which is presumed to be closer to neutrally stable. In this study the stability of ENSO and the Atlantic Niño mode is compared via an analysis of the Bjerknes stability index. This index is based on recharge oscillator theory and can be interpreted as the growth rate for coupled modes of ocean–atmosphere variability. Using observational data, an ocean reanalysis product, and output from an ocean general circulation model, the individual terms of the Bjerknes index are calculated for the first time for the Atlantic and then compared to results for the Pacific. Positive thermocline feedbacks in response to wind stress forcing favor anomaly growth in both basins, but they are twice as large in the Pacific compared to the Atlantic. Thermocline feedback is related to the fetch of the zonal winds, which is much greater in the equatorial Pacific than in the equatorial Atlantic due to larger basin size. Negative feedbacks are dominated by thermal damping of sea surface temperature anomalies in both basins. Overall, it is found that both ENSO and the Atlantic Niño mode are damped oscillators, but the Atlantic is more strongly damped than the Pacific primarily because of the weaker thermocline feedback.

Beschreibung: Port calls: Saßnitz

Beschreibung: Kiel – Visby – Ventspils ‐ Kiel: 29. 05. – 21. 06. 2010 Cruise ALKOR 355 (AL‐355) was carried out within the framework of the EU FP‐7 project “HYPOX” (In situ monitoring of oxygen depletion in hypoxic ecosystems of coastal and open seas, and land‐locked water bodies). The scientific objectives focused on redox‐dependent nutrient and nitrogen cycles in the benthic boundary layer of the suboxic to anoxic Gotland Basin. The scientific party comprised of scientist from the IFM‐GEOMAR, Kiel (Germany). Main objectives were to study release rates and inventories of nutrients and nitrogen compounds under varying degrees of bottom water oxygenation in the anoxic to suboxic eastern Gotland Basin (central Baltic). Another goal was to quantify source/sink mechanisms of the benthic boundary layer for nitrogen species and nutrients under changing oxygen conditions to assess potential feedbacks on the upper mixed layer processes. In order to obtain these goal, we monitored the water column, sampled surface sediments and deployed several landers along a transect from oxygenated to suboxic and anoxic to sulfide benthic boundary layer conditions in the Latvian EEZ of the eastern Gotland Basin in a depth range from 49m to 235m.

Beschreibung: This study aimed to examine interactive effects between ocean acidification and temperature on the photosynthetic and growth performance of Neosiphonia harveyi. N. harveyi was cultivated at 10 and 17.5 °C at present (~380 µatm), expected future (~800 µatm), and high (~1500 µatm) pCO2. Chlorophyll a fluorescence, net photosynthesis, and growth were measured. The state of the carbon-concentrating mechanism (CCM) was examined by pH-drift experiments (with algae cultivated at 10 °C only) using ethoxyzolamide, an inhibitor of external and internal carbonic anhydrases (exCA and intCA, respectively). Furthermore, the inhibitory effect of acetazolamide (an inhibitor of exCA) and Tris (an inhibitor of the acidification of the diffusive boundary layer) on net photosynthesis was measured at both temperatures. Temperature affected photosynthesis (in terms of photosynthetic efficiency, light saturation point, and net photosynthesis) and growth at present pCO2, but these effects decreased with increasing pCO2. The relevance of the CCM decreased at 10 °C. A pCO2 effect on the CCM could only be shown if intCA and exCA were inhibited. The experiments demonstrate for the first time interactions between ocean acidification and temperature on the performance of a non-calcifying macroalga and show that the effects of low temperature on photosynthesis can be alleviated by increasing pCO2. The findings indicate that the carbon acquisition mediated by exCA and acidification of the diffusive boundary layer decrease at low temperatures but are not affected by the cultivation level of pCO2, whereas the activity of intCA is affected by pCO2. Ecologically, the findings suggest that ocean acidification might affect the biogeographical distribution of N. harveyi.

Beschreibung: The vast majority of microbes inhabiting the subseafloor remain uncultivated and their energy sources unknown. Thus, a focus of ocean drilling expeditions over the past decade has been to characterize the distribution of microbes associated with specific metabolic reactions. An important question has been whether microbes involved in key microbial processes, such as sulfate reduction and methanogenesis, differ fundamentally from their counterparts in surface environments. To this end, functional genes of anaerobic methane cycling (mcrA), sulfate reduction (dsrAB), acetogenesis (fhs), and dehalorespiration (rdhA) have been examined. A compilation of existing functional gene data suggests that subseafloor microbes involved in anaerobic methane cycling, sulfate reduction, acetogenesis, and dehalorespiration are not fundamentally different from their counterparts in the surface world. Moreover, quantifications of mcrA and dsrAB suggest that, unless the majority of subseafloor microbes involved in methane cycling and sulfate reduction are too genetically divergent to be detected with conventional methods, these processes only support a small fraction (〈 1%) of total microbial biomass in the deep biosphere. Ecological explanations for the observed trends, target processes and methods for future investigations, and strategies for tackling the unresolved issue of microbial contamination in samples obtained by ocean drilling are discussed.

Beschreibung: Epibiotic biofilms have the potential to control major aspects of the biology and ecology of their hosts. Their composition and function may thus be essential for the health of the host. We tested the influence of salinity on the composition of epibacterial communities associated with the brown macroalga Fucus vesiculosus. Algal individuals were incubated at three salinities (5, 19, and 25) for 14days and nonliving reference substrata (stones) were included in the experiment. Subsequently, the composition of their surface-associated bacterial communities was analyzed by 454 pyrosequencing of 16S rRNA gene sequences. Redundancy analysis revealed that the composition of epiphytic and epilithic communities significantly differed and were both affected by salinity. We found that 5% of 2494 epiphytic operational taxonomic units at 97% sequence similarity were responsible for the observed shifts. Epibacterial -diversity was significantly lower at salinity 5 but did not differ between substrata. Our results indicate that salinity is an important factor in structuring alga-associated epibacterial communities with respect to composition and/or diversity. Whether direct or indirect mechanisms (via altered biotic interactions) may have been responsible for the observed shifts is discussed.

Beschreibung: Port calls: Saßnitz

Beschreibung: Port calls: Stettin, Poland, 15.05.2011 – 16.05.2011

Beschreibung: In spite of considerable insights into the microbial diversity of marine sponges, quantitative information on microbial abundances and community composition remains scarce. Here, we established qPCR assays for the specific quantification of four bacterial phyla of representative sponge symbionts as well as the kingdoms Eubacteria and Archaea. We could show that the 16S rRNA gene numbers of Archaea, Chloroflexi, and the candidate phylum Poribacteria were 4–6 orders of magnitude higher in high microbial abundance (HMA) than in low microbial abundance (LMA) sponges and that actinobacterial 16S rRNA gene numbers were 1–2 orders higher in HMA over LMA sponges, while those for Cyanobacteria were stable between HMA and LMA sponges. Fluorescence in situ hybridization of Aplysina aerophoba tissue sections confirmed the numerical dominance of Chloroflexi, which was followed by Poribacteria. Archaeal and actinobacterial cells were detected in much lower numbers. By use of fluorescence-activated cell sorting as a primer- and probe-independent approach, the dominance of Chloroflexi, Proteobacteria, and Poribacteria in A. aerophoba was confirmed. Our study provides new quantitative insights into the microbiology of sponges and contributes to a better understanding of the HMA/LMA dichotomy.

Beschreibung: Datum: 01.09.2011  Fahrtgebiet: Strander Bucht  Fahrtleiter: Dr. Jörg Bialas, IFM-GEOMAR

Beschreibung: The prasinophyte order Mamiellales contains several widespread marine picophytoplankton (≤2 μm diameter) taxa, including Micromonas and Ostreococcus. Complete genome sequences are available for two Micromonas isolates, CCMP1545 and RCC299. We performed in silico analyses of nitrogen transporters and related assimilation genes in CCMP1545 and RCC299 and compared these with other green lineage organisms as well as Chromalveolata, fungi, bacteria, and archaea. Phylogenetic reconstructions of ammonium transporter (AMT) genes revealed divergent types contained within each Mamiellales genome. Some were affiliated with plant and green algal AMT1 genes and others with bacterial AMT2 genes. Land plant AMT2 genes were phylogenetically closer to archaeal transporters than to Mamiellales AMT2 genes. The Mamiellales represent the first green algal genomes to harbor AMT2 genes, which are not found in Chlorella and Chlamydomonas or the chromalveolate algae analyzed but are present in oomycetes. Fewer nitrate transporter (NRT) than AMT genes were identified in the Mamiellales. NRT1 was found in all but CCMP1545 and showed highest similarity to Mamiellales and proteobacterial NRTs. NRT2 genes formed a bootstrap-supported clade basal to other green lineage organisms. Several nitrogen-related genes were colocated, forming a nitrogen gene cluster. Overall, RCC299 showed the most divergent suite of nitrogen transporters within the various Mamiellales genomes, and we developed TaqMan quantitative polymerase chain reaction primer-probes targeting a subset of these, as well as housekeeping genes, in RCC299. All those investigated showed expression either under standard growth conditions or under nitrogen depletion. Like other recent publications, our findings show a higher degree of "mixed lineage gene affiliations" among eukaryotes than anticipated, and even the most phylogenetically anomalous versions appear to be functional. Nitrogen is often considered a regulating factor for phytoplankton populations. This study provides a springboard for exploring the use and functional diversification of inorganic nitrogen transporters and related genes in eukaryotic phytoplankton. © 2010 The Author.