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  • Southern Ocean  (12)
  • Mixotrophy
  • Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu  (9)
  • Frontiers Media  (6)
  • 2015-2019  (15)
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  • 1
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: CTD Biogenic Silica
    Description: Biogenic silica concentrations collected from CTD casts during RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018 For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/780191
    Description: NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) OPP-1644073
    Keywords: Southern Ocean ; Ross Sea ; Biogenic silica
    Repository Name: Woods Hole Open Access Server
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  • 2
    Publication Date: 2022-10-31
    Description: Dataset: NBP1608 TMs: stations
    Description: Dissolved trace metal concentrations from depth profiles during RVIB Nathaniel B. Palmer cruise NBP16-08 in the Southern Ocean from 2016-09-11 to 2016-10-10. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/781773
    Description: NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) OPP-1443483, NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) OPP-1443474, NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) OPP-1443646
    Description: 2020-06-01
    Keywords: Trace metals ; Southern Ocean
    Repository Name: Woods Hole Open Access Server
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  • 3
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: Pigments HPLC
    Description: Algal pigment concentrations as measured by HPLC from RVIB Nathaniel B. Palmer cruise in the Ross Sea, Southern Ocean from 2017-2018. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/778881
    Description: NSF Office of Polar Programs (formerly NSF PLR) (NSF OPP) OPP-1644073
    Keywords: Southern Ocean ; Ross Sea ; Phytoplankton pigments ; Chlorophyll
    Repository Name: Woods Hole Open Access Server
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  • 4
    Publication Date: 2022-05-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Newman, L., Heil, P., Trebilco, R., Katsumata, K., Constable, A., van Wijk, E., Assmann, K., Beja, J., Bricher, P., Colemans, R., Costa, D., Diggs, S., Farneti, R., Fawcett, S., Gille, S. T., Hendry, K. R., Henley, S., Hofmann, E., Maksym, T., MazIoff, M., Meijers, A., Meredith, M. M., Moreau, S., Ozsor, B., Robertson, R., Schloss, I., Schofield, O., Shi, J., Sikes, E., Smith, I. J., Swart, S., Wahlin, A., Williams, G., Williams, M. J. M., Herraiz-Borreguero, L., Kern, S., Liesers, J., Massom, R. A., Melbourne-Thomas, J., Miloslavich, P., & Spreen, G. Delivering sustained, coordinated, and integrated observations of the Southern Ocean for global impact. Frontiers in Marine Science, 6, (2019): 433, doi:10.3389/fmars.2019.00433.
    Description: The Southern Ocean is disproportionately important in its effect on the Earth system, impacting climatic, biogeochemical, and ecological systems, which makes recent observed changes to this system cause for global concern. The enhanced understanding and improvements in predictive skill needed for understanding and projecting future states of the Southern Ocean require sustained observations. Over the last decade, the Southern Ocean Observing System (SOOS) has established networks for enhancing regional coordination and research community groups to advance development of observing system capabilities. These networks support delivery of the SOOS 20-year vision, which is to develop a circumpolar system that ensures time series of key variables, and delivers the greatest impact from data to all key end-users. Although the Southern Ocean remains one of the least-observed ocean regions, enhanced international coordination and advances in autonomous platforms have resulted in progress toward sustained observations of this region. Since 2009, the Southern Ocean community has deployed over 5700 observational platforms south of 40°S. Large-scale, multi-year or sustained, multidisciplinary efforts have been supported and are now delivering observations of essential variables at space and time scales that enable assessment of changes being observed in Southern Ocean systems. The improved observational coverage, however, is predominantly for the open ocean, encompasses the summer, consists of primarily physical oceanographic variables, and covers surface to 2000 m. Significant gaps remain in observations of the ice-impacted ocean, the sea ice, depths 〉2000 m, the air-ocean-ice interface, biogeochemical and biological variables, and for seasons other than summer. Addressing these data gaps in a sustained way requires parallel advances in coordination networks, cyberinfrastructure and data management tools, observational platform and sensor technology, two-way platform interrogation and data-transmission technologies, modeling frameworks, intercalibration experiments, and development of internationally agreed sampling standards and requirements of key variables. This paper presents a community statement on the major scientific and observational progress of the last decade, and importantly, an assessment of key priorities for the coming decade, toward achieving the SOOS vision and delivering essential data to all end-users.
    Description: PH was supported by the Australian Government’s Cooperative Research Centers Program through the Antarctica Climate and Ecosystems Cooperative Research Centre, and the International Space Science Institute’s team grant #406. This work contributes to the Australian Antarctica Science projects 4301 and 4390.
    Keywords: Southern Ocean ; observations ; modeling ; ocean–climate interactions ; ecosystem-based management ; long-term monitoring ; international coordination
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-10-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in [citation], doi:[doi]. Swart, S., Gille, S. T., Delille, B., Josey, S., Mazloff, M., Newman, L., Thompson, A. F., Thomson, J., Ward, B., du Plessis, M. D., Kent, E. C., Girton, J., Gregor, L., Heil, P., Hyder, P., Pezzi, L. P., de Souza, R. B., Tamsitt, V., Weller, R. A., & Zappa, C. J. Constraining Southern Ocean air-sea-ice fluxes through enhanced observations. Frontiers in Marine Science, 6, (2019): 421, doi:10.3389/fmars.2019.00421.
    Description: Air-sea and air-sea-ice fluxes in the Southern Ocean play a critical role in global climate through their impact on the overturning circulation and oceanic heat and carbon uptake. The challenging conditions in the Southern Ocean have led to sparse spatial and temporal coverage of observations. This has led to a “knowledge gap” that increases uncertainty in atmosphere and ocean dynamics and boundary-layer thermodynamic processes, impeding improvements in weather and climate models. Improvements will require both process-based research to understand the mechanisms governing air-sea exchange and a significant expansion of the observing system. This will improve flux parameterizations and reduce uncertainty associated with bulk formulae and satellite observations. Improved estimates spanning the full Southern Ocean will need to take advantage of ships, surface moorings, and the growing capabilities of autonomous platforms with robust and miniaturized sensors. A key challenge is to identify observing system sampling requirements. This requires models, Observing System Simulation Experiments (OSSEs), and assessments of the specific spatial-temporal accuracy and resolution required for priority science and assessment of observational uncertainties of the mean state and direct flux measurements. Year-round, high-quality, quasi-continuous in situ flux measurements and observations of extreme events are needed to validate, improve and characterize uncertainties in blended reanalysis products and satellite data as well as to improve parameterizations. Building a robust observing system will require community consensus on observational methodologies, observational priorities, and effective strategies for data management and discovery.
    Description: SS was funded by a Wallenberg Academy Fellowship (WAF 2015.0186). EK was funded by the NERC ORCHESTRA Project (NE/N018095/1). LP was funded by the Advanced Studies in Oceanography of Medium and High Latitudes (CAPES 23038.004304/2014-28) and the Research Productivity Program (CNPq 304009/2016-4). BdS was a research associate at the F.R.S-FNRS. PeH was supported by the Australian Antarctic Science Projects 4301 and 4390, and the Australian Government’s Cooperative Research Centres Programme through the Antarctic Climate and Ecosystems Cooperative Research Centre and the International Space Science Institute Project 406. SG and MM were funded by National Science Foundation awards OCE-1658001 and PLR-1425989. AT was supported by NASA (NNX15AG42G) and NSF (OCE-1756956).
    Keywords: Air-sea/air-sea-ice fluxes ; Southern Ocean ; Ocean-atmosphere interaction ; Climate ; Ocean-ice interaction
    Repository Name: Woods Hole Open Access Server
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  • 6
    Publication Date: 2018-07-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Marine Science 5 (2018): 241, doi:10.3389/fmars.2018.00241.
    Description: Cryptophyte algae are globally distributed photosynthetic flagellates found in freshwater, estuarine, and neritic ecosystems. While cryptophytes can be highly abundant and are consumed by a wide variety of protistan predators, few studies have sought to quantify in situ grazing rates on their populations. Here we show that autumnal grazing rates on in situ communities of cryptophyte algae in Chesapeake Bay are high throughout the system, while growth rates, particularly in the lower bay, were low. Analysis of the genetic diversity of cryptophyte populations within dilution experiments suggests that microzooplankton may be selectively grazing the fastest-growing members of the population, which were generally Teleaulax spp. We also demonstrate that potential grazing rates of ciliates and dinoflagellates on fluorescently labeled (FL) Rhodomonas salina, Storeatula major, and Teleaulax amphioxeia can be high (up to 149 prey predator−1 d−1), and that a Gyrodinium sp. and Mesodinium rubrum could be selective grazers. Potential grazing was highest for heterotrophic dinoflagellates, but due to its abundance, M. rubrum also had a high overall impact. This study reveals that cryptophyte algae in Chesapeake Bay can experience extremely high grazing pressure from phagotrophic protists, and that this grazing likely shapes their community diversity.
    Description: The authors thank the National Science Foundation (OCE 1031718 and 1436169) for providing support for this research.
    Keywords: Cryptophytes ; Mixotrophy ; Grazing ; Chesapeake Bay ; Dinoflagellates ; Mesodinium rubrum
    Repository Name: Woods Hole Open Access Server
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  • 7
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Marine Science 5 (2018): 13, doi:10.3389/fmars.2018.00013.
    Description: Identifying putative mixotrophic protist species in the environment is important for understanding their behavior, with the recovery of these species in culture essential for determining the triggers of feeding, grazing rates, and overall impact on bacterial standing stocks. In this project, mixotroph abundances determined using tracer ingestion in water and sea ice samples collected in the Ross Sea, Antarctica during the summer of 2011 were compared with data from the spring (Ross Sea) and fall (Arctic) to examine the impacts of bacterivory/mixotrophy. Mixotrophic nanoplankton (MNAN) were usually less abundant than heterotrophs, but consumed more of the bacterial standing stock per day due to relatively higher ingestion rates (1–7 bacteria mixotroph−1 h−1 vs. 0.1–4 bacteria heterotroph−1 h−1). Yet, even with these high rates observed in the Antarctic summer, mixotrophs appeared to have a smaller contribution to bacterivory than in the Antarctic spring. Additionally, putative mixotroph taxa were identified through incubation experiments accomplished with bromodeoxyuridine-labeled bacteria as food, immunoprecipitation (IP) of labeled DNA, and amplification and high throughput sequencing of the eukaryotic ribosomal V9 region. Putative mixotroph OTUs were identified in the IP samples by taxonomic similarity to known phototroph taxa. OTUs that had increased abundance in IP samples compared to the non-IP samples from both surface and chlorophyll maximum (CM) depths were considered to represent active mixotrophy and include ones taxonomically similar to Dictyocha, Gymnodinium, Pentapharsodinium, and Symbiodinium. These OTUs represent target taxa for isolation and laboratory experiments on triggers for mixotrophy, to be combined with qPCR to estimate their abundance, seasonal distribution and potential impact.
    Description: This work was supported by National Science Foundation Grants OPP-0838955 (RG) and OPP-0838847 (RS).
    Keywords: Protist ; Diversity ; Mixotrophy ; Ross Sea ; Amplicon sequencing
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  • 8
    Publication Date: 2022-05-25
    Description: © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Marine Science 5 (2018): 273, doi:10.3389/fmars.2018.00273.
    Description: Mixotrophic flagellates can comprise significant proportions of plankton biomass in marine ecosystems. Despite the growing recognition of the importance of this ecological strategy, and the identification of major environmental factors controlling phagotrophic behavior (light and nutrients), the physiological and molecular mechanisms underlying mixotrophic behavior are still unclear. In this study, we performed RNA-Seq transcriptomic analysis for two mixotrophic prasinophytes, Micromonas polaris and Pyramimonas tychotreta, under dissolved nutrient regimes that altered their ingestion of bacteria prey. Though the strains examined were polar isolates, both belong to genera with widespread distribution. Our aim was to characterize the transcriptomes of these two non-model phytoflagellates, identify transcripts consistent with phagotrophic activity and assess their differential expression in response to nutrient stress. De novo assembly of the transcriptomes yielded large numbers of novel coding transcripts with no known match within public databases. A summary of the transcripts by Gene Ontology terms showed many expected expression patterns, including genes involved in photosynthetic pathways and enzymes implicated in nutrient uptake pathways. Searches of KEGG databases identified several genes associated with intra-cellular digestive pathways actively transcribed in both prasinophytes. Differential expression analysis showed a larger response in P. tychotreta, where 23,373 genes were up-regulated and 1,752 were down-regulated in the low nutrient treatment when phagotrophy was enhanced. In contrast, in M. polaris, low nutrient treatments resulted in up-regulation of 314 transcripts while down-regulating 371. With respect to phagotrophic-related expression, 37 genes were co-expressed in both P. tychotreta and M. polaris, and although the response was less pronounced in M. polaris, it is consistent with differences in observed ingestion behavior. This study presents the first genomic data for Pyramimonas tychotreta, and also contributes to the limited available data for Micromonas polaris. Furthermore, it provides insight into the presence of genes associated with phagocytosis within the Prasinophyceae and contributes to the understanding of potential target genes required for the construction of a complete model of gene regulation of phagocytic behavior in algae.
    Description: The Owlsnest Super-Computing Cluster at Temple University is funded by a National Science Foundation Grant CNS-09-58854. The CUNY HPCC is operated by the College of Staten Island and funded, in part, by grants from the City of New York, State of New York, CUNY Research Foundation, and National Science Foundation Grants CNS-0958379, CNS-0855217, and ACI 1126113. Support for this work was also supplied by National Science Foundation grants PLR-1341362 (RG), PLR-1603538 (RS), and PLR-1603833 (RG).
    Keywords: Mixotrophy ; Pyramimonas ; Micromonas ; RNA-Seq ; Transcriptomics ; Phagotrophy
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  • 9
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: McMurdo Sound CTDs
    Description: CTD data were collected as part of an ecosystem study in McMurdo Sound, which is located at the southern extent of the Ross Sea in the Southern Ocean. The major goal of this multi-disciplinary project was to assess the influence of top−down forcing (predation) on pelagic zooplankton and fish. During the first year (3 November 2012 – 21 January 2013), the CTD was deployed through ice core holes in the fast ice (sea ice attached to land), sampling from near surface to depths between 97 and 175 m. Stations were located along a transect in the middle of McMurdo Sound, perpendicular to the fast ice edge. In the second year (17 November 2014 – 1 January 2015), CTD casts were deployed between 100 and 254 m in depth, at stations along the fast ice edge, and along three transects into the fast ice along the eastern side of McMurdo Sound (Ross Island), in the middle of the Sound, and on the western side of the Sound. Chlorophyll fluorescence sensor measurements on the CTD casts were only made during the 2014/2015 field expedition. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/680929
    Description: NSF Division of Polar Programs (NSF PLR) PLR-0944511
    Keywords: Temperature ; Salinity ; PH ; Chlorophyll fluorescence ; Southern Ocean
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  • 10
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: McMurdo Sound chlorophyll
    Description: Discrete chlorophyll a data were collected as part of an ecosystem study in McMurdo Sound, which is located at the southern extent of the Ross Sea in the Southern Ocean. The major goal of this multi-disciplinary project was to assess the influence of top−down forcing (predation) on pelagic zooplankton and fish. Samples were collected using Niskin water bottles deployed through the fast ice (sea ice attached to land) during two spring/summer seasons: 3 November 2012 – 21 January 2013 and17 November 2014 – 1 January 2015. Water samples were collected at the surface and in the chlorophyll maximum, when present, as determined by a fluorescence sensor during a CTD cast. During 2012/2013, stations were located along a transect in the middle of McMurdo Sound, perpendicular to the fast ice edge. During 2014/2015, stations were located along the fast ice edge, and along three transects into the fast ice along the eastern side of the McMurdo Sound (Ross Island), in the middle of the Sound, and on the western side of the Sound. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/679685
    Description: NSF Division of Polar Programs (NSF PLR) PLR-0944511
    Keywords: Chlorophyll a ; Phytoplankton ; Southern Ocean
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  • 11
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: CTD_MOCNESS1
    Description: CTD data from MOCNESS-1m tows in the Southern Ocean around the South Shetland Islands and Palmer Archipelago, Western Antarctic Peninsula in November 2011. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/488871
    Description: NSF Antarctic Sciences (NSF ANT) ANT-1044982
    Keywords: Southern Ocean ; CTD ; MOCNESS ; Zooplankton
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  • 12
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: CTD data from LMG1110
    Description: Hydrographic data from 20 CTD casts in the Southern Ocean around the South Shetland Islands and Palmer Archipelago, Western Antarctic Peninsula in November 2011. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/559174
    Description: NSF Antarctic Sciences (NSF ANT) ANT-1044982
    Keywords: CTD ; Temperature ; Conductivity ; Depth ; Southern Ocean
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  • 13
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: alongtrack
    Description: This alongtrack data set consists of a single file produced for each day of the LMG1110 cruise, from 11/2/2011 to 12/1/2011. The LMG alongtrack data acquisition systems continuously log data from a suite of instruments throughout the cruise. Data were obtained primarily by applying calibrations to raw data and decimating to whole point intervals. However, several fields are derived measurements from more than a single raw input. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/3636
    Description: NSF Antarctic Sciences (NSF ANT) ANT-1044982
    Keywords: Southern Ocean ; Event log
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  • 14
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-05-26
    Description: Dataset: event log LMG11-10
    Description: The event log to record all instrument deployments during the cruise. For a complete list of measurements, refer to the supplemental document 'Field_names.pdf', and a full dataset description is included in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: http://www.bco-dmo.org/dataset/3565
    Description: NSF Antarctic Sciences (NSF ANT) ANT-1044982
    Keywords: Southern Ocean
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  • 15
    Publication Date: 2022-05-26
    Description: © The Author(s), 2015. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 6 (2015): 1090, doi:10.3389/fmicb.2015.01090.
    Description: Antarctica polynyas support intense phytoplankton blooms, impacting their environment by a substantial depletion of inorganic carbon and nutrients. These blooms are dominated by the colony-forming haptophyte Phaeocystis antarctica and they are accompanied by a distinct bacterial population. Yet, the ecological role these bacteria may play in P. antarctica blooms awaits elucidation of their functional gene pool and of the geochemical activities they support. Here, we report on a metagenome (~160 million reads) analysis of the microbial community associated with a P. antarctica bloom event in the Amundsen Sea polynya (West Antarctica). Genomes of the most abundant Bacteroidetes and Proteobacteria populations have been reconstructed and a network analysis indicates a strong functional partitioning of these bacterial taxa. Three of them (SAR92, and members of the Oceanospirillaceae and Cryomorphaceae) are found in close association with P. antarctica colonies. Distinct features of their carbohydrate, nitrogen, sulfur and iron metabolisms may serve to support mutualistic relationships with P. antarctica. The SAR92 genome indicates a specialization in the degradation of fatty acids and dimethylsulfoniopropionate (compounds released by P. antarctica) into dimethyl sulfide, an aerosol precursor. The Oceanospirillaceae genome carries genes that may enhance algal physiology (cobalamin synthesis). Finally, the Cryomorphaceae genome is enriched in genes that function in cell or colony invasion. A novel pico-eukaryote, Micromonas related genome (19.6 Mb, ~94% completion) was also recovered. It contains the gene for an anti-freeze protein, which is lacking in Micromonas at lower latitudes. These draft genomes are representative for abundant microbial taxa across the Southern Ocean surface.
    Description: This work was performed with financial support from NSF Antarctic Sciences awards ANT-1142095 to AP.
    Keywords: Southern Ocean ; Amundsen Sea Polynya ; Phytoplankton bloom ; Phaeocystis ; Micromonas ; Microbial communities ; Metagenomics ; Genome reconstruction
    Repository Name: Woods Hole Open Access Server
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