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  • in-cell NMRnuclear magnetic resonancecellular structural biologycellular environmentprotein interactions  (2)
  • Abyss  (1)
  • International Union of Crystallography (IUCr)  (2)
  • American Association for the Advancement of Science  (1)
  • Essen : Verl. Glückauf
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • 2015-2019  (3)
  • 2005-2009
Collection
Keywords
Publisher
  • International Union of Crystallography (IUCr)  (2)
  • American Association for the Advancement of Science  (1)
  • Essen : Verl. Glückauf
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
Years
  • 2015-2019  (3)
  • 2005-2009
Year
  • 1
    Publication Date: 2022-05-26
    Description: © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Science Advances 3 (2017): e1601426, doi:10.1126/sciadv.1601426.
    Description: Southern Ocean abyssal waters, in contact with the atmosphere at their formation sites around Antarctica, not only bring signals of a changing climate with them as they move around the globe but also contribute to that change through heat uptake and sea level rise. A repeat hydrographic line in the Indian sector of the Southern Ocean, occupied three times in the last two decades (1994, 2007, and, most recently, 2016), reveals that Antarctic Bottom Water (AABW) continues to become fresher (0.004 ± 0.001 kg/g decade−1), warmer (0.06° ± 0.01°C decade−1), and less dense (0.011 ± 0.002 kg/m3 decade−1). The most recent observations in the Australian-Antarctic Basin show a particularly striking acceleration in AABW freshening between 2007 and 2016 (0.008 ± 0.001 kg/g decade−1) compared to the 0.002 ± 0.001 kg/g decade−1 seen between 1994 and 2007. Freshening is, in part, responsible for an overall shift of the mean temperature-salinity curve toward lower densities. The marked freshening may be linked to an abrupt iceberg-glacier collision and calving event that occurred in 2010 on the George V/Adélie Land Coast, the main source region of bottom waters for the Australian-Antarctic Basin. Because AABW is a key component of the global overturning circulation, the persistent decrease in bottom water density and the associated increase in steric height that result from continued warming and freshening have important consequences beyond the Southern Indian Ocean.
    Description: The 2016 I08S cruise and the analysis and science performed at sea, as well as the individual principal investigators were funded through multiple National Oceanic and Atmospheric Administration (NOAA) and NSF grants including NSF grant OCE-1437015. The research for this article was mainly completed at sea. For land-based work, V.V.M. relied on her postdoctoral funding through NSF grant OCE-1435665, and A.M.M. was supported in part by NSF grant OCE-1356630 and NOAA grant NA11OAR4310063.
    Keywords: Salinity ; AABW ; Changes ; Water masses ; T-S properties ; Iceberg ; Calving ; Antartica ; Abyss ; Climate change
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 2
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    International Union of Crystallography (IUCr)
    In: IUCrJ
    Publication Date: 2017-02-15
    Description: Classical structural biology approaches allow structural characterization of biological macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth of structural data available and to technological and methodological advances, the interest of the research community is gradually shifting from pure structural determination towards the study of functional aspects of biomolecules. Therefore, a cellular structural approach is ideally needed to characterize biological molecules, such as proteins, in their native cellular environment and the functional processes that they are involved in. In-cell NMR is a new application of high-resolution nuclear magnetic resonance spectroscopy that allows structural and dynamical features of proteins and other macromolecules to be analyzed directly in living cells. Owing to its challenging nature, this methodology has shown slow, but steady, development over the past 15 years. To date, several in-cell NMR approaches have been successfully applied to both bacterial and eukaryotic cells, including several human cell lines, and important structural and functional aspects have been elucidated. In this topical review, the major advances of in-cell NMR are summarized, with a special focus on recent developments in eukaryotic and mammalian cells.
    Keywords: in-cell NMRnuclear magnetic resonancecellular structural biologycellular environmentprotein interactions
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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  • 3
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    International Union of Crystallography (IUCr)
    In: IUCrJ
    Publication Date: 2017-02-24
    Keywords: in-cell NMRnuclear magnetic resonancecellular structural biologycellular environmentprotein interactions
    Electronic ISSN: 2052-2525
    Topics: Geosciences , Physics
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