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  • 1
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    Springer Nature | Springer International Publishing
    Publication Date: 2024-04-05
    Description: Chilean Patagonia, located at the southwestern tip of South America, is one of the last regions on earth where highly intact environments predominate. With a coastline that extends along some 100,000 km of fjords, channels, and islands, it has one of the world´s most extensive marine-terrestrial interfaces. Local place-based and Indigenous cultures and management practices are a vital presence across the region, while the long and rich history of conservation efforts have resulted in officially protected areas covering over 50% of the land and 41% of the coastal-marine area. However, Chilean Patagonia is increasingly facing anthropogenic pressures associated with increased infrastructure and access, salmon aquaculture, extractive industries, and the spread of invasive exotic species. Despite widespread recognition that Chilean Patagonia represents a unique global reservoir of socio-natural heritage, to date there has been no region-wide assessment of the scientific evidence of the conservation status of its ecosystems or the priorities for their effective conservation. Conservation in Chilean Patagonia: Assessing the state of knowledge, opportunities, and challenges is the first book to gather and synthesize the available scientific and socio-environmental information related to Patagonian conservation. It presents the collaborative work of 68 researchers and local experts, representing a range of specialties and perspectives, including: biology, ecology, socio-ecology, fisheries, aquaculture, anthropology, economics, geography, tourism, cryosphere, oceanography, climate and global change. The book’s 18 chapters focus on the status of key ecosystems and conservation tools, and provide recommendations toward the construction of a renewed, inclusive, and integrated conservation agenda for the Chilean Patagonian region. It provides an essential primer for anyone interested in the future of this ecologically vital region, as well as lessons on interdisciplinary collaboration and integrated analysis of conservation issues useful for conservation practitioners and scholars. This is an open access book. This book is a translation of an original Spanish edition. The translation was done with the help of artificial intelligence (machine translation by the service DeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation.
    Keywords: Ecology ; Limnology ; Oceanography ; Biodiversity ; Marine-terrestrial Interface ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAF Ecological science, the Biosphere ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RN The environment::RNK Conservation of the environment ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RB Earth sciences::RBK Hydrology and the hydrosphere::RBKC Oceanography (seas and oceans) ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RG Geography::RGM Biogeography ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RN The environment::RNC Applied ecology::RNCB Biodiversity
    Language: English
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  • 2
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    Springer Nature | Springer International Publishing
    Publication Date: 2024-01-16
    Description: Chilean Patagonia, located at the southwestern tip of South America, is one of the last regions on earth where highly intact environments predominate. With a coastline that extends along some 100,000 km of fjords, channels, and islands, it has one of the world´s most extensive marine-terrestrial interfaces. Local place-based and Indigenous cultures and management practices are a vital presence across the region, while the long and rich history of conservation efforts have resulted in officially protected areas covering over 50% of the land and 41% of the coastal-marine area. However, Chilean Patagonia is increasingly facing anthropogenic pressures associated with increased infrastructure and access, salmon aquaculture, extractive industries, and the spread of invasive exotic species. Despite widespread recognition that Chilean Patagonia represents a unique global reservoir of socio-natural heritage, to date there has been no region-wide assessment of the scientific evidence of the conservation status of its ecosystems or the priorities for their effective conservation. Conservation in Chilean Patagonia: Assessing the state of knowledge, opportunities, and challenges is the first book to gather and synthesize the available scientific and socio-environmental information related to Patagonian conservation. It presents the collaborative work of 68 researchers and local experts, representing a range of specialties and perspectives, including: biology, ecology, socio-ecology, fisheries, aquaculture, anthropology, economics, geography, tourism, cryosphere, oceanography, climate and global change. The book’s 18 chapters focus on the status of key ecosystems and conservation tools, and provide recommendations toward the construction of a renewed, inclusive, and integrated conservation agenda for the Chilean Patagonian region. It provides an essential primer for anyone interested in the future of this ecologically vital region, as well as lessons on interdisciplinary collaboration and integrated analysis of conservation issues useful for conservation practitioners and scholars. This is an open access book. This book is a translation of an original Spanish edition. The translation was done with the help of artificial intelligence (machine translation by the service DeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation.
    Keywords: Ecology ; Limnology ; Oceanography ; Biodiversity ; Marine-terrestrial Interface ; bic Book Industry Communication::R Earth sciences, geography, environment, planning::RN The environment::RNK Conservation of the environment ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSA Life sciences: general issues::PSAF Ecological science, the Biosphere ; bic Book Industry Communication::R Earth sciences, geography, environment, planning::RG Geography::RGM Biogeography ; bic Book Industry Communication::R Earth sciences, geography, environment, planning::RN The environment::RNC Applied ecology::RNCB Biodiversity
    Language: English
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  • 3
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    transcript Verlag | transcript Verlag
    Publication Date: 2024-03-22
    Description: Gene gelten im Allgemeinen als die Essenz eines Lebewesens, die all seine charakteristischen Eigenschaften bestimmt. Aus biologischer Sicht trifft diese Vorstellung jedoch längst nicht mehr zu. Im Mittelpunkt dieses Buches steht daher die Frage, warum und wie das essentialistische Denken die gesellschaftliche Wahrnehmung biologischer Forschungsprojekte immer noch beeinflusst. Anhand aktueller Erkenntnisse der Genetik und Epigenetik geht Kirsten Schmidt auf die Suche nach einer neuen Interpretation des Genbegriffs im Zeitalter der Postgenomik. Das Verständnis von Genen als dynamischen Prozessen erweist sich dabei als eine fruchtbare Alternative zum Essentialismus.
    Keywords: Essentialismus ; Gen ; Philosophie ; Epigenetik ; Biologie ; Genetik ; Postgenomik ; Leben ; Wissenschaft ; Wissenschaftsphilosophie ; Naturphilosophie ; Wissenschaftssoziologie ; Soziologie ; Philosophy ; Biology ; Life ; Science ; Philosophy of Science ; Philosophy of Nature ; Sociology of Science ; Sociology ; bic Book Industry Communication::P Mathematics & science::PD Science: general issues::PDA Philosophy of science ; bic Book Industry Communication::H Humanities::HP Philosophy::HPJ Philosophy: metaphysics & ontology ; bic Book Industry Communication::P Mathematics & science::PD Science: general issues::PDR Impact of science & technology on society
    Language: German
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  • 4
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    Masaryk University Press | Masaryk University
    Publication Date: 2024-04-04
    Description: Title in English: History of the Institute of Biology, Faculty of Medicine, Masaryk University A representative book on the history and present of one of the oldest institutes of the Faculty of Medicine of Masaryk University, which has been contributing significantly to the development of biological sciences since its inception.
    Keywords: Historical sciences ; Historyof Medicine ; health care ; Personalities ; Natural science ; Biology ; thema EDItEUR::P Mathematics and Science::PD Science: general issues::PDX History of science
    Language: Czech
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  • 5
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2023-11-30
    Description: Active flow control (AFC) utilizes local active perturbations to induce changes in global flow behavior that result in aero/hydrodynamic performance improvement. It has been a vibrant research area with potential applications in a wide range of engineering fields. This Special Issue is a collection of 11 excellent research papers published in Actuators, showcasing and discussing new advances in both fundamental and applied AFC technologies.
    Keywords: Active flow control ; Actuators ; Aerodynamics ; Synthetic jets&nbsp ; bic Book Industry Communication::T Technology, engineering, agriculture::TB Technology: general issues
    Language: English
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  • 6
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    Scientific Committee on Oceanic Research | Newark, Delaware, USA
    Publication Date: 2023-03-09
    Description: This proceeding summarizes the discussions during the 48th SCOR Annual Meeting held in hybrid format from Busan, Korea, between the 4-6 of October of 2022. This proceeding also provides the links for all the background information for the meeting, including the proposals for new working groups, the reports from current SCOR working groups, projects, capacity development activities, and the reports of affiliated and partner organizations all of which were traditionally included in the SCOR Annual meeting background book until 2019. All of these can also be accessed online through the SCOR website at: https://scor-int.org/events/scor-2022-annual-meeting/.
    Description: Published
    Description: Non Refereed
    Keywords: Ocean science ; SCOR Annual Meeting 2022 ; Oceanography
    Repository Name: AquaDocs
    Type: Book/Monograph/Conference Proceedings
    Format: 70pp.
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  • 7
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    Logos Verlag Berlin | Logos Verlag Berlin
    Publication Date: 2024-04-04
    Description: In Zeiten, in denen die Komplexität gesellschaftlicher, politischer, wirtschaftlicher und technologischer Probleme zunehmend steigt, ist der Erwerb einer naturwissenschaftlichen Grundbildung für die persönliche Meinungsbildung von zentraler Bedeutung. Hierzu gehört das Erlernen von naturwissenschaftlichen Erkenntnismethoden wie dem Experimentieren. Erkenntnisse zu experimentierspezifischen Kompetenzen zeigen, dass Lernende über unterschiedliche Vorstellungen bezüglich des Experimentierens verfügen. Diese zeigen sich in unterschiedlichen Vorgehensweisen, die mehr oder weniger stark von der Vorgehensweise in realer wissenschaftlicher Forschung abweichen. Ziel der vorliegenden Studie ist eine differenzierte Erfassung und Analyse individueller Prozessstrukturen sowie prozessbezogener Niveaustufen von Experimentierprozessen Lehramtsstudierender der Biologie. Die Ergebnisse zeigen, dass die meisten Experimentierprozesse nicht, wie in idealisierten Modellen angenommen, einem linearen Prozessverlauf verlaufen, sondern wiederholte Wechsel zwischen den Experimentierphasen aufweisen. Insbesondere die Durchführung nimmt hier eine zentrale Stellung ein. Die Vernetzung der Experimentierphasen ist unterschiedlich ausgeprägt und steht in einem positiven Zusammenhang mit der Qualität eines Experimentierprozesses. Die prozessbezogenen Niveaustufen weisen Ausprägungen über alle Niveaus hinweg auf. Aus den Ergebnissen werden Hinweise zur Gestaltung von Unterricht und universitärer Lehre sowie Implikationen für die fachdidaktische Forschung abgeleitet.
    Keywords: Science ; Science ; Life Sciences ; Biology ; Education ; thema EDItEUR::P Mathematics and Science::PD Science: general issues ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences ; thema EDItEUR::J Society and Social Sciences::JN Education
    Language: German
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  • 8
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    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: Some sixty years after the experimental flights of the North American X-15 hypersonic rocket-powered aircraft, sustained hypervelocity travel is still the next frontier in high-speed transportation. Today, there is much excitement and interest regarding hypersonic vehicles. In fact, many aerospace agencies, large industries, and several start-ups are involved in design activities and experimental campaigns both in wind tunnels and in-flight with full-scale experimental flying test beds and prototypes to make hypersonic travel almost as easy and convenient as airliner travel. Achieving this goal will radically revolutionize the future of civil transportation. This book contains valuable contributions that focus on various design issues related to hypersonic aircraft.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science::TGMF Engineering: Mechanics of fluids::TGMF1 Aerodynamics
    Language: English
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  • 9
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    Logos Verlag
    Publication Date: 2024-04-05
    Description: New technologies have revealed previously unknown and invisible parts of the human body and made it visible at the molecular level, revealing in turn more detailed structures and arrangements than those which were previously available. In doing so, in many ways they refine, expand, and even completely overturn forms of contemporary knowledge. This book maps the shifts and blurring of boundaries in contemporary bioscientific discourse. The authors of its chapters trace the shifts of boundaries in terms of the gradual blurring of the validity of established concepts, interpretive frameworks, and standards of judgment, which are analysed from ontological, gnoseological, ethical, and social perspectives. At the same time, they also map the blurring of boundaries in terms of the interdisciplinary crossing of boundaries between various scientific and artistic disciplines. The shifting of boundaries ultimately forms a part of these boundaries’ definition; upon the basis of a rationally guided discussion, these shifts can be guided and corrected so as to avoid any irreversible damage. Jana Tomašovičová is a philosopher with a special interest in contemporary philosophy and bioethics. She analyses the impact of biotechnology on traditional social, ethical, and anthropological concepts and their relevance in new conditions. She is an associate professor at the Faculty of Arts, University of Ss. Cyril and Methodius in Trnava, Slovakia. During her bioethics research, she conducted short research stays at the universities of Bonn, Heidelberg, Tübingen, and Zürich.
    Keywords: Science ; Life Sciences ; Biology ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
    Language: English
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  • 10
    Publication Date: 2023-06-29
    Description: Climate change is altering our planet and the effects are felt from the highest mountains to the deepest parts of the ocean. While the world seeks to hold warming to 1.5°C, it is vital that we take steps now to protect some of the Earth’s natural jewels and to preserve them for future generations. The UNESCO World Heritage List includes the world’s most iconic marine protected areas, recognised by the international community for their outstanding biodiversity, beauty, geology and natural habitats. Beginning with Australia’s Great Barrier Reef in 1981, the List has since expanded to include a global network of 50 ocean places of Outstanding Universal Value (OUV), from the tropics to the poles, each of which helps to secure the future of our marine ecosystems. Inclusion on the List is only the start of the work needed to protect these sites from warming seas and shifting weather. Indeed, some 70% of the marine World Heritage sites are currently under threat from climate change, according to the 2020 IUCN World Heritage Outlook. Under a business-as-usual emissions scenario, World Heritage Listed coral reef systems are expected to cease to exist by 2100. Action is necessary not just to protect these sites, but because between them they host over 20% of the world’s blue carbon ecosystems - representing critical carbon sinks - and serve as refuges for vulnerable and threatened species. Managers, scientists, and funders are enthusiastic and willing to help us achieve healthy oceans and marine World Heritage sites. But how? The 2021 UNESCO science assessment survey of marine World Heritage sites indicates that nearly 75% of sites lack knowledge on how to protect their OUV against the impacts from climate change. And about two thirds lack the tools to understand how climate change will impact their biodiversity and ecosystem functioning.We must find evidence-based solutions to address these questions and to help sites plan for the uncertain future. In 2017, the United Nations General Assembly proclaimed that 2021-2030 would serve as the United Nations Decade of Ocean Science for Sustainable Development (or ‘Ocean Decade’). The Ocean Decade provides a global framework to harness science to sustainably manage the oceans. Marine World Heritage sites are identified as priority areas in the Implementation Plan of the Ocean Decade. The Decade offers a way to convene diverse actors to co-design and co-deliver knowledge that will address scientific questions about the vulnerable sites, to plan the right response and to put them on a path to a sustainable future. Climate change is a complex challenge, and we must use the best and most up-to-date research and data to guide our actions. Collecting ocean science data and identifying trends are critical to local management teams. Without this baseline knowledge, including where iconic species live or trends in environmental and socio-economic variables, effective management decisions cannot be made in ways that will ensure sites’ protection 10 or 20 years from now. Yet despite their iconic status, many marine World Heritage sites lack essential capacity, technology and resources to generate and process data, including the baseline observations crucial to gather the evidence to plan future steps. For many sites, budgets have not risen while challenges grow exponentially. In response, UNESCO is launching a call for increased and strategic investment in the ocean science needed to safeguard marine World Heritage sites. The ocean is a vast place and there is much to do. Within the framework of the Ocean Decade, this roadmap aims to help provide focus, to ensure research is carried out and used in an efficient, effective and sustainable way. It identifies knowledge that site managers and scientists need to conserve marine World Heritage sites and foster resilient marine ecosystems, highlights the value of science-based decision making, and tackles some key obstacles including resources and capacity. This roadmap outlines key information to assess climate vulnerability, including on the use of targeted science to underpin conservation and management efforts. It also highlights current gaps in science capacity and infrastructure, including data collection and interpretation. Finally, it explores the technology and capacity required for action and the sustainable finance and resources needed to support the necessary research. Marine World Heritage sites face a critical moment in time and we must act now. By developing this roadmap within the framework of the Ocean Decade, we have the chance to generate ‘the science we need for the ocean we want’ and preserve marine World Heritage sites and their services for future generations. This roadmap seeks to offer that help, by showing managers, supporters, and funders how science and research can be more cost-effectively directed to some of the most pressing problems. Together we can steer a path to a resilient and sustainable future, for the next decade and beyond.
    Description: OPENASFA INPUT Suggested citation: UNESCO. 2021. Ocean Science Roadmap for UNESCO Marine World Heritage in the context of the United Nations Decade of Ocean Science for Sustainable Development (2021-2030). Paris, France
    Description: Published
    Description: Not Known
    Keywords: Underwater archeology ; Cultural Heritage ; Marine Sciences ; Oceanography ; Climate Change ; Sustainable Development ; United Nations Decade of Ocean Science for Sustainable Development ; Ocean Decade
    Repository Name: AquaDocs
    Type: Report
    Format: 16pp.
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  • 11
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    United Nations and United Nations Educational, Scientific and Cultural Organization | Paris, France
    Publication Date: 2023-06-29
    Description: This information document presents the STAB’s Strategic framework on engaging in the UN Decade of Ocean Science for Sustainable Development 2021-2030, elaborated following a working meeting held between the STAB and the Intergovernmental Oceanographic Commission in November 2020.
    Description: OPENASFA INPUT
    Description: Published
    Description: Non Refereed
    Keywords: Underwater archeology ; Oceanographic data ; Oceanography ; United Nations Decade of Ocean Science for Sustainable Development ; Ocean Decade
    Repository Name: AquaDocs
    Type: Conference Material
    Format: 8pp.
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  • 12
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    IOC of UNESCO | Paris, France
    Publication Date: 2023-06-29
    Description: Brochure for conferences, meetings, etc under the subject: 'United Nations Decade of Ocean Science for Sustainable Development'
    Description: Korea Institute of Ocean Science & Technology
    Description: OPENASFA INPUT Brochure
    Description: Published
    Description: Not Known
    Keywords: United Nations Decade of Ocean Science for Sustainable Development ; Sustainable Development ; Oceanography ; Ocean Decade ; Sustainable Development Goals
    Repository Name: AquaDocs
    Type: Report
    Format: 8pp.
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  • 13
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    UNESCO-IOC | Paris, France
    Publication Date: 2024-05-27
    Description: GenOcean instils a feeling of unity - it's not them and us - we are all in this together. Connecting with the audience, gaining their trust, and allowing them to seamlessly collaborate is essential. But to connect, we have to be on the same wavelength. This campaign identity guide serves as the starting point to create a unified, inspiring and determined campaign that aims to inspire everyday actions to restore and protect the ocean. The following pages contain inspiration, guidelines, and handy tips to communicate our values, realize our vision, and reinforce the GenOcean campaign. Thank you for helping achieve the GenOcean mission to restore and protect the ocean. Welcome to GenOcean.
    Description: Government of Japan
    Description: From the People of Japan
    Description: University of Education, Culture, Sports, Science and Technology, Japan
    Description: OPENASFA INPUT For bibliographic purposes, this publication should be cited as follows: IOC-UNESCO. GenOcean Campaign Identity. Paris. 2022. 72 pp. (The Ocean Decade Series, 33).
    Description: Published
    Description: Not Known
    Keywords: Branding ; Oceanography ; Ocean Literacy ; Environmental awareness ; United Nations Decade of Ocean Science for Sustainable Development ; Ocean Decade
    Repository Name: AquaDocs
    Type: Report
    Format: 72pp.
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  • 14
    Publication Date: 2024-05-27
    Description: In 2016, the first World Ocean Assessment of the United Nations stated that humankind was running out of time to start managing the ocean sustainably. This alarming conclusion poses a question to our civilization: is there a way to reverse the decline in ocean health while continuing to rely on the ocean for our ever-increasing needs, particularly under a changing climate? The proclamation by the United Nations General Assembly in December 2017 of the UN Decade of Ocean Science for Sustainable Development, 2021–2030 (hereafter, the ‘Ocean Decade’) is based on the informed conviction of UN Member States that indeed, this opportunity still exists, and that, furthermore, ocean science needs to play a central role in this process. Ocean science is broad: it encompasses natural and social science disciplines, local and indigenous knowledge; it includes the science-policy and science-innovation interfaces, as well as technology and infrastructure. At the beginning of the third millennium, ocean science is largely competent for diagnosing problems. However, its ability to offer solutions of direct relevance to sustainable development requires a massive upgrade. This need is particularly urgent against the current backdrop of the global COVID-19 pandemic and accelerating climate change. The pandemic has, once again, highlighted the importance of science and knowledge for decision-making and policy. As the world adjusts to a new normal, the ocean will need to play a central role in post-pandemic recovery efforts. However, for this to occur, there needs to be a nothing short of a revolution in ocean science. The Ocean Decade will create a paradigm shift in the generation of qualitative and quantitative ocean knowledge – including from currently data-poor regions, such as the deep ocean, coastal areas where much of the human interaction with the ocean is concentrated, and the polar regions – to inform the development of solutions that contribute to the 2030 Agenda for Sustainable Development. The Ocean Decade aims to catalyse the human behaviour change required for the successful implementation of these solutions. Guided by the United Nations Convention on the Law of the Sea (UNCLOS), the Ocean Decade will generate the data, information and knowledge needed for more robust science-informed policies and stronger science-policy interfaces at global, regional, national and even local levels, leading to improved integrated ocean management and development of a sustainable ocean economy. The Ocean Decade will support numerous UN entities to fulfil their ocean-related mandates. In our information-centred, internet-linked society, the Ocean Decade will support ocean data, information and knowledge systems to evolve into a much higher level of readiness, accessibility, and interoperability. The scale of such efforts will need to be exponentially greater than anything seen to date. An equally transformational part of the Ocean Decade is about humanity and our relationship with the ocean. Understanding of the value of the ocean can be nurtured through ocean literacy efforts among diverse stakeholder groups. Holders of indigenous and local knowledge will work as essential partners of the Ocean Decade and will contribute to highlighting the multitude of cultural values of the ocean. Equity, inclusiveness, respect, fairness and scientific integrity are core principles of the Ocean Decade. The Ocean Decade will systematically identify and dismantle barriers to achieving gender, geographic and generational balance so that no one is left behind. Everyone should be able to benefit from ocean science, including Small Island Developing States, Least Developed Countries and Landlocked Developing Countries. Designing and delivering ocean science that focuses on user needs and adopts relevant mechanisms for uptake will be a key metamorphosis to be achieved between 2021 and 2030. Its scale will be unprecedented. Multiple stakeholders are expected to engage and start collaborating outside their traditional communities. Knowledge generators and users will engage in an iterative process of co-design and co-delivery of ocean science. This will create new groupings of actors from natural, social science and humanity disciplines, business and industry, governments, UN entities, intergovernmental organizations (IGOs), NGOs and civil society, educators, early career ocean professionals, ocean sports and recreation organizations, arts and cultural communities, and indigenous and local knowledge holders. Partnerships and active communication will be at the heart of the Ocean Decade. This Decade is not the first to take on the challenge of ocean science. In 1971–1980, earlier generations embarked on the International Decade of Ocean Exploration. As part of that Decade, groundbreaking collaborative research projects occurred. Many of which, such as the World Ocean Circulation Experiment, permanently changed the face of ocean exploration. However, one crucial difference remains between the two Decades: in the 1970s, the aim was to generate the ‘science we want’. In today’s world, we no longer have that luxury, and the current Decade is resolutely focused on the ‘science we need’. The Implementation Plan for such a major undertaking as the Ocean Decade cannot be, and is not, prescriptive. Rather, it provides a framework for transformational action that will build on existing achievements and deliver action across geographies, sectors, disciplines and generations. I hope you, as a reader and an Ocean Decade stakeholder, will share the overall strategic vision and approach of the Ocean Decade as described in the Implementation Plan. With your engagement and your support, the impact of the Ocean Decade will be much bigger than the sum of its parts and together we will be able to create the science we need for the ocean we want.
    Description: OPENASFA INPUT he document should be cited as follows: UNESCO-IOC (2021). The United Nations Decade of Ocean Science for Sustainable Development (2021-2030) Implementation Plan. UNESCO, Paris (IOC Ocean Decade Series, 20.).
    Description: Published
    Description: Not Known
    Keywords: United Nations Decade of Ocean Science for Sustainable Development ; Implementation Plan ; Oceanography ; Sustainable Development ; Science and Development
    Repository Name: AquaDocs
    Type: Report
    Format: 56pp.
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  • 15
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    UNESCO-IOC | Paris, France
    Publication Date: 2024-05-27
    Description: On 5 December 2017, the United Nations (UN) declared that a Decade of Ocean Science for Sustainable Development (‘Ocean Decade’) would be held from 2021 to 2030. The Ocean Decade provides a common framework to ensure that ocean science can underpin the achievement of the 17 Sustainable Development Goals (SDGs) and complementary global and regional policy frameworks including the United Nations Framework Convention on Climate Change. The Ocean Decade provides a ‘once-in- a-lifetime’ opportunity to create a new foundation across the science-policy interface to strengthen the management of the ocean and coasts for the benefit of humanity and to mitigate the impacts of climate change. The Ocean Decade Implementation Plan outlines ten Decade Challenges, representing the most immediate and pressing needs of the Decade, which will guide stakeholders as they come together to co-design and co-deliver a wide range of Decade Actions that will be implemented the ocean-climate nexus is embodied in Challenge No. 5 and is reflected in a number of the other Challenges over the next ten years. The Intergovernmental Oceanographic Commission of UNESCO (IOC-UNESCO) has been mandated to coordinate implementation of the Ocean Decade. The Ocean Decade will provide the data, knowledge and capacity to address science and knowledge gaps needed to make informed policy decisions. The United Nations (UN) General Assembly clearly recognizes the societal benefits of a healthy ocean and the need to work across UN entities to achieve this goal. Working in coordination with the UN Framework Convention on Climate Change (UNFCCC), the Ocean Decade will contribute to addressing these societal challenges for example by providing the sound science needed to reflect ocean considerations in Nationally Determined Contributions (NDCs) of the Paris Agreement on Climate Change. In the Ocean and Climate Change Dialogue1, the UNFCCC reaffirmed that science must be strengthened and central to this process. The complementary structure of the Ocean Decade Action Framework to the goals of COP26 will allow for meaningful contributions in achieving successful outcomes.
    Description: MEXT
    Description: From the People of Japan
    Description: OPENASFA INPUT UNESCO-IOC.2021. The Ocean Decade at COP26 of the United Nations Framework Convention on Climate Change. Paris, UNESCO. (The Ocean Decade Series, 31) - (IOC/2021/ODS/31)
    Description: Published
    Description: Not Known
    Keywords: UN Decade of Ocean Science ; COP26 ; Oceanography ; Sustainable Development ; Ocean Decade
    Repository Name: AquaDocs
    Type: Report
    Format: 8pp.
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  • 16
    Publication Date: 2024-05-27
    Description: The Implementation Plan of the UN Decade of Ocean Science for Sustainable Development (the Ocean Decade) calls for ‘transformative science’ and a ‘revolution in how that science is produced, used and disseminated’. The solutions-oriented nature of the Ocean Decade creates the conditions for this revolution because it provides a convening framework to foster the partnerships and develop the scientific knowledge needed to catalyse transformative ocean science solutions for sustainable development, connecting people and our ocean. While there is widespread enthusiasm to engage in this collaborative venture, there is a need to build capacity and common understanding in how to create co-designed solutions that could bring about the desired transformation in ocean management. This discussion note ‘Co-designing the Science We Need for the Ocean We Want: Guidance and Recommendations for Collaborative Approaches to Designing & Implementing Decade Actions’ aims to address this in a holistic manner. It was inspired by discussions held during a series of global and regional webinars in late 2020 that brought together 2,100 individuals from around the world to bring to life the notion of collaborative, co-designed science and identify the key obstacles, challenges and opportunities. The note offers a solid starting point for stakeholders on the: what, why and how they can join efforts to co-design salient, credible and legitimate ocean knowledge solutions which deliver on the Ocean Decade’s vision of ‘the science we need for the ocean we want’.
    Description: Government of Sweden
    Description: International Science Council
    Description: OPENASFA INPUT For bibliographic purposes, this publication should be cited as follows: IOC-UNESCO. 2021. Co-designing the Science We Need for the Ocean We Want: Guidance and Recommendations for Collaborative Approaches to Designing & Implementing Decade Actions. Paris, UNESCO. (The Ocean Decade Series, 29).
    Description: Published
    Description: Not Known
    Keywords: Ocean Decade ; United Nations Decade of Ocean Science for Sustainable Development ; Sustainable Development ; Oceans ; Oceanography ; Scientific cooperation ; International Cooperation
    Repository Name: AquaDocs
    Type: Report
    Format: 16pp.
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  • 17
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    Taylor & Francis | CRC Press
    Publication Date: 2023-09-12
    Description: The complex regulations of the Endangered Species Act established by the U.S. Fish and Wildlife Service can be challenging for environmental professionals who must comply with them or assist clients in compliance. This is true especially for those without a background in biology or ecology. The Endangered Species Act: History, Implementation, Successes, and Controversies discusses the Act using clear scientific prose that all professionals whose activities fit into the ESA compliance process can readily comprehend, including those with limited education in science. The book begins by exploring the deeply rooted history of the Endangered Species Act, which extends back decades preceding its enactment in 1973. It continues with a discussion of the basic scientific theory underlying the Act and provides an overview of its key regulations. The author also examines the Act in the context of other key environmental planning statutes such as the National Environmental Policy Act and the Clean Water Act, especially Section 404 of the Clean Water Act, which relates specifically to wetlands. The remainder of the book details the regulatory processes faced by other government agencies and private developers who must routinely ensure that their actions comply with the Endangered Species Act. It concludes with a broad discussion of current controversies associated with the Act and how those controversies might ultimately change how environmental practitioners will have to comply with the Act in the future. The book is neither a defense of the Endangered Species Act and its associated regulations nor a call to repeal or modify the Act or regulations. The presentation is factual and avoids the hype and hyperbole commonly directed at the Act by both environmental activists and deregulation proponents. Readers will gain a solid understanding of how the Act was established, what goals were envisioned by its framers, how current environmental practice under the Act has been shaped, and how those practices might be changed in the future.
    Keywords: Environment law ; Applied ecology ; Biology ; life sciences ; Zoology & animal sciences ; bic Book Industry Communication::L Law::LN Laws of Specific jurisdictions::LNK Environment, transport & planning law::LNKJ Environment law ; bic Book Industry Communication::R Earth sciences, geography, environment, planning::RN The environment::RNC Applied ecology ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences::PSV Zoology & animal sciences
    Language: English
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  • 18
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    UCL Press
    Publication Date: 2024-03-23
    Description: Bloomsbury Scientists is the story of the network of scientists and artists living in a square mile of London before and after the First World War. This inspired group of men and women viewed creativity and freedom as the driving force behind nature, and each strove to understand this in their own inventive way. Their collective energy changed the social mood of the era and brought a new synthesis of knowledge to ideas in science and art. Class barriers were threatened as power shifted from the landed oligarchy to those with talent and the will to make a difference. A time of unexpected opportunities, from the new disciplines of Genetics and Ecology to Post-Impressionism and beyond, Michael Boulter seamlessly weaves together the stories originating from Bloomsbury’s laboratories, libraries and studios. He narrates the breakthroughs of scientists such as Ray Lankester and Marie Stopes alongside the creative outputs of H. G. Wells and Virginia Woolf, among many others, and intricately connects them all through personal friendships, grievances, quarrels and affections. Bloomsbury Scientists offers a fresh and crucial perspective on this history at a time when the complex relationship between science and art continues to be debated.
    Keywords: london ; history of science ; bloomsbury ; scientists ; Biology ; Charles Darwin ; Evolution ; Francis Galton ; thema EDItEUR::1 Place qualifiers::1D Europe::1DD Western Europe::1DDU United Kingdom, Great Britain ; thema EDItEUR::N History and Archaeology::NH History::NHD European history ; thema EDItEUR::P Mathematics and Science::PD Science: general issues::PDX History of science
    Language: English
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  • 19
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    Frontiers Media SA
    Publication Date: 2024-04-04
    Description: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
    Keywords: polyoxometalates ; Catalysis ; Biology ; Energy ; Materials Science ; magnetic materials ; active matter ; Water-oxidation ; Keggin ; supramolecular chemistry ; thema EDItEUR::P Mathematics and Science::PD Science: general issues
    Language: English
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  • 20
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    Frontiers Media SA
    Publication Date: 2024-04-04
    Description: The Frontiers in Chemistry Editorial Office team are delighted to present the inaugural “Frontiers in Chemistry: Rising Stars” article collection, showcasing the high-quality work of internationally recognized researchers in the early stages of their independent careers. All Rising Star researchers featured within this collection were individually nominated by the Journal’s Chief Editors in recognition of their potential to influence the future directions in their respective fields. The work presented here highlights the diversity of research performed across the entire breadth of the chemical sciences, and presents advances in theory, experiment and methodology with applications to compelling problems. This Editorial features the corresponding author(s) of each paper published within this important collection, ordered by section alphabetically, highlighting them as the great researchers of the future. The Frontiers in Chemistry Editorial Office team would like to thank each researcher who contributed their work to this collection. We would also like to personally thank our Chief Editors for their exemplary leadership of this article collection; their strong support and passion for this important, community-driven collection has ensured its success and global impact.
    Keywords: Green and Sustainable Chemistry ; Analytical Chemistry ; Theoretical and Computational Chemistry ; Polymer Chemistry ; Medicinal and Pharmaceutical Chemistry ; Organic Chemistry ; Nanoscience ; Catalysis and Photocatalysis ; Supramolecular Chemistry ; Electrochemistry ; Inorganic Chemistry ; Chemical Biology ; thema EDItEUR::P Mathematics and Science::PD Science: general issues
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  • 21
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    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: Aerodynamics, the study of air motion around solid objects, allows us to understand and measure the dominating forces acting on aircrafts, buildings, bridges, automobiles, and other structures. The forces that result in an aircraft overcoming gravity and drag are called thrust and lift. Various parameters such as geometrical configurations of objects, as well as physical properties of air, which may be functions of position and time, affect those forces. This book covers some of the latest studies regarding the application of the principles of aerodynamics to the design of many different engineered objects. This book will be of interest to mechanical and aerospace engineering students, academics, and researchers who are looking for new insights into this fascinating branch of fluid mechanics.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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  • 22
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    MDPI - Multidisciplinary Digital Publishing Institute
    Publication Date: 2024-04-05
    Description: Nanofibers, particularly those of a carbonaceous content, have received increased interest in the past two decades due to their outstanding physico-chemical characteristics and their possibility to form and contribute towards a plethora of potentially advantageous materials for consumer, industrial and medical applications. Despite this, and together with the numerous research studies and published articles that have sought to investigate these aspects, the potential impact of CNTs is still not understood. Whether or not nanofibers may be able to provide a sophisticated alternative to conventional materials is still debatable, whilst their effects upon both environmental and human health are highly equivocal. How nanofibers are conceived can determine how they may interact with different environments, such as the human body. Understanding each key step of the synthesis and production of nanofibers to their use within potential applications is therefore essential in gaining an insight into how they may be perceived by any biological system and environment. Thus, obtaining such information will enable all scientific communities to begin to realize the potential advantages posed by nanofibers. The aim of this Special Issue therefore, was to provide a collective overview of nanofibers; ‘from synthesis to application’. The Issue particularly focuses upon carbon-based nanofibers, but also highlights alternative nanofiber types. Emphasis is given holistically, with articles discussing the production routes of nanofibers, their plight during their life-cycle (origin to applied form and effects over time), as well as how nanofibers could either incite conflict, or provide aid to human and environmental health.
    Keywords: QD1-999 ; Toxicology ; Chemistry ; Biology ; Material Science ; Nanofibers ; Nanotechnology ; thema EDItEUR::P Mathematics and Science::PN Chemistry
    Language: English
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  • 23
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    Frontiers Media SA
    Publication Date: 2024-04-04
    Description: This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact
    Keywords: Self-recruitment ; Connectivity ; Oceanography ; Behaviour ; Scales ; thema EDItEUR::P Mathematics and Science::PD Science: general issues ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RB Earth sciences::RBK Hydrology and the hydrosphere::RBKC Oceanography (seas and oceans)
    Language: English
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  • 24
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    Springer Nature
    Publication Date: 2024-04-05
    Description: This open access book discusses biogeochemical processes relevant to carbon and aims to provide readers, graduate students and researchers, with insight into the functioning of marine ecosystems. A carbon centric approach has been adopted, but other elements are included where relevant or needed. The book focuses on concepts and quantitative understanding of primary production, organic matter mineralization and sediment biogeochemistry. The impact of biogeochemical processes on inorganic carbon dynamics and organic matter transformation are also discussed.
    Keywords: Earth sciences ; Geobiology ; Oceanography ; Geochemistry ; Aquatic ecology  ; Ecosystems ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences::PSA Life sciences: general issues::PSAF Ecological science, the Biosphere ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RB Earth sciences::RBG Geology, geomorphology and the lithosphere::RBGK Geochemistry ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RB Earth sciences::RBK Hydrology and the hydrosphere::RBKC Oceanography (seas and oceans) ; thema EDItEUR::R Earth Sciences, Geography, Environment, Planning::RG Geography::RGM Biogeography
    Language: English
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  • 25
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    Springer Nature
    Publication Date: 2023-02-02
    Description: Oceanography; Biogeosciences; Geochemistry
    Keywords: Oceanography ; Biogeosciences ; Geochemistry ; bic Book Industry Communication::K Economics, finance, business & management::KC Economics::KCN Environmental economics ; bic Book Industry Communication::R Earth sciences, geography, environment, planning
    Language: English
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  • 26
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    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: Aerodynamics, from a modern point of view, is a branch of physics that study physical laws and their applications, regarding the displacement of a body into a fluid, such concept could be applied to any body moving in a fluid at rest or any fluid moving around a body at rest. This Book covers a small part of the numerous cases of stationary and non stationary aerodynamics; wave generation and propagation; wind energy; flow control techniques and, also, sports aerodynamics. It's not an undergraduate text but is thought to be useful for those teachers and/or researchers which work in the several branches of applied aerodynamics and/or applied fluid dynamics, from experiments procedures to computational methods.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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  • 27
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    transcript Verlag
    Publication Date: 2021-02-10
    Description: Wahrnehmung, Kognition und Ästhetik lassen sich als differente Verarbeitungs- und Ausdrucksformen einer biologischen Basisausstattung des Menschen verstehen. Ob diese stabil sind oder ob sie kulturell überformt oder gar transformiert werden, bildet die zentrale medienanthropologische Fragestellung des Bandes. Dabei wird geprüft, welcher Art die wechselseitigen Impulse sind, die zu kulturellen und biologischen Veränderungen führen können, welche Wirkung sie besitzen und welche Dauer ihnen zukommt. Diese Fragen richten sich nicht allein auf Prozesse wahrnehmungstechnischer, kognitiver oder ästhetischer Habitualisierungen, sondern thematisieren deren physiologische Voraussetzungen. Mit der Beteiligung ausgewiesener Forscher aus den Bereichen Biologie, Medizin und Hirnforschung erschließt dieser Band Neuland im Grenzgebiet der Natur- und Kulturwissenschaften.
    Keywords: Wahrnehmung ; Kognition ; Ästhetik ; Medien ; Biologie ; Hirnforschung ; Medienästhetik ; Medienwissenschaft ; Aesthetics ; Media ; Biology ; Media Aesthetics ; Media Studies ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFD Media studies
    Language: German
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  • 28
    Publication Date: 2024-04-05
    Description: The Great Lagoon is a central part of the Szczecin Lagoon, a major component in the Odra River estuary system. It is also an important European natural heritage site and one of the largest resting places for migratory birds in the Baltic Sea area. The first part of Wolnomiejski's and Witek’s book gives a thorough overview of the most up-to-date knowledge of this region, including the assessment of its biological production. Based on these findings authors develop a food web model of the Polish part of the Szczecin Lagoon, identifying a total of 45 trophic-functional components. The model describes a variety of features ranging from the magnitude of consumption, to the amount of unassimilated food and export of individual system components, and serves as an invaluable source, helping researchers to estimate various ecological indicators of The Great Lagoon’s ecosystem.
    Keywords: QH301-705.5 ; Biology ; Natural Sciences ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences
    Language: English
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  • 29
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    Universitätsverlag Göttingen
    Publication Date: 2024-04-04
    Description: This bibliography documents the printed works of Johann Friedrich Blumenbach (1752–1840) and makes them accessible for research purposes. Blumenbach was Professor of Medicine and Natural History at the University of Göttingen. He was one of the leading exponents of the revolutionary change of the geo-biological concept of the world at the turn of the 18th to the 19th century. His works exemplarily show the interactions between the life sciences, the humanities and the social sciences of his day.
    Keywords: History of Universities ; Bibliography ; Biology ; History of Science ; Darwinism ; thema EDItEUR::P Mathematics and Science::PD Science: general issues ; thema EDItEUR::J Society and Social Sciences::JN Education::JNM Higher education, tertiary education ; thema EDItEUR::G Reference, Information and Interdisciplinary subjects::GB Encyclopaedias and reference works::GBC Reference works::GBCR Bibliographies, catalogues ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences ; thema EDItEUR::P Mathematics and Science::PD Science: general issues::PDX History of science
    Language: German
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  • 30
    Publication Date: 2022-04-28
    Description: For more than 40 years, Jesper Hoffmeyer has been committed to the idea of developing “a semiotics of nature, or biosemiotics as he chose to call this effort, that could intelligibly explain how all the phenomena of inherent meaning and signification in living nature – from the lowest level of sign processes in unicellular organisms to the cognitive and social behavior of animals – can emerge from a universe that was not so organized and meaningful from the very beginning” (Emmeche et al. 2002: 41). In this volume, over 80 world-class scholars from more than 20 countries select a short quotation taken from any of Jesper Hoffmeyer’s texts and provide their scholarly commentary upon that passage – whether in the form of an analytical explication, a critical disagreement or a conceptual extension – that as they feel asks the questions that need to be asked, proposes the ideas that need to be proposed, or that draws out the implications that need to be so explicitly drawn out, germane to the claims of the selected passage. At once a celebration and a serious academic development of the work of Jesper Hoffmeyer, this landmark volume marks the occasion of his 70th birthday on February 21, 2012.
    Keywords: jesper hoffmeyer ; semiotics ; biosemiotics ; Biology ; Charles Sanders Peirce ; Evolution ; Semiosis ; bic Book Industry Communication::G Reference, information & interdisciplinary subjects::GT Interdisciplinary studies::GTE Semiotics / semiology
    Language: English
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  • 31
    Publication Date: 2024-04-08
    Description: The name DGGTB (Deutsche Gesellschaft für Geschichte und Theorie der Biologie; German Society for the History and Theory of Biology) reflects recent history as well as German tradition. The Society is a relatively late addition to a series of German societies of science and medicine that began with the »Deutsche Gesellschaft für Geschichte der Medizin und der Naturwissenschaften«, founded in 1910 by Leipzig University's Karl Sudhoff (1853-1938), who wrote: »We want to establish a ,German' society in order to gather German-speaking historians together in our special disciplines so that they form the core of an international society...«. Yet Sudhoff, at this time of burgeoning academic internationalism, was »quite willing« to accommodate the wishes of a number of founding members and »drop the word German in the title of the Society and have it merge with an international society«. The founding and naming of the Society at that time derived from a specific set of historical circumstances, and the same was true some 80 years later when in 1991, in the wake of German reunification, the »Deutsche Gesellschaft für Geschichte und Theorie der Biologie« was founded. From the start, the Society has been committed to bringing studies in the history and philosophy of biology to a wide audience, using for this purpose its Jahrbuch für Geschichte und Theorie der Biologie. Parallel to the Jahrbuch, the Verhandlungen zur Geschichte und Theorie der Biologie has become the by now traditional medium for the publication of papers delivered at the Society's annual meetings. In 2005 the Jahrbuch was renamed Annals of the History and Philosophy of Biology, reflecting the Society's internationalist aspirations in addressing comparative biology as a subject of historical and philosophical studies.
    Description: The name DGGTB (Deutsche Gesellschaft für Geschichte und Theorie der Biologie; German Society for the History and Theory of Biology) reflects recent history as well as German tradition. The Society is a relatively late addition to a series of German societies of science and medicine that began with the »Deutsche Gesellschaft für Geschichte der Medizin und der Naturwissenschaften«, founded in 1910 by Leipzig University's Karl Sudhoff (1853-1938), who wrote: »We want to establish a ,German' society in order to gather German-speaking historians together in our special disciplines so that they form the core of an international society...«. Yet Sudhoff, at this time of burgeoning academic internationalism, was »quite willing« to accommodate the wishes of a number of founding members and »drop the word German in the title of the Society and have it merge with an international society«. The founding and naming of the Society at that time derived from a specific set of historical circumstances, and the same was true some 80 years later when in 1991, in the wake of German reunification, the »Deutsche Gesellschaft für Geschichte und Theorie der Biologie« was founded. From the start, the Society has been committed to bringing studies in the history and philosophy of biology to a wide audience, using for this purpose its Jahrbuch für Geschichte und Theorie der Biologie. Parallel to the Jahrbuch, the Verhandlungen zur Geschichte und Theorie der Biologie has become the by now traditional medium for the publication of papers delivered at the Society's annual meetings. In 2005 the Jahrbuch was renamed Annals of the History and Philosophy of Biology, reflecting the Society's internationalist aspirations in addressing comparative biology as a subject of historical and philosophical studies.
    Keywords: Biology ; Historical studies ; Philosophical studies ; Maggi ; Pavia ; bic Book Industry Communication::H Humanities::HP Philosophy::HPC History of Western philosophy ; bic Book Industry Communication::P Mathematics & science::PS Biology, life sciences ; bic Book Industry Communication::W Lifestyle, sport & leisure::WN Natural history ; thema EDItEUR::Q Philosophy and Religion::QD Philosophy::QDH Philosophical traditions and schools of thought ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences ; thema EDItEUR::W Lifestyle, Hobbies and Leisure::WN Nature and the natural world: general interest
    Language: German
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  • 32
    Publication Date: 2022-04-28
    Description: At the start of the twenty-first century, warnings have been raised in some quarters about how – by intent or by mishap – advances in biotechnology and related fields could aid the spread of disease. Science academics, medical organisations, governments, security analysts, and others are among those that have sought to raise concern. Education and Ethics in the Life Sciences examines a variety of attempts to bring greater awareness to security concerns associated with the life sciences. It identifies lessons from practical initiatives across a wide range of national contexts as well as more general reflections about education and ethics. The eighteen contributors bring together perspectives from a diverse range of fields – including politics, virology, sociology, ethics, security studies, microbiology, and medicine – as well as their experiences in universities, think tanks and government. In offering their assessment about what must be done and by whom, each chapter addresses a host of challenging practical and conceptual questions. Education and Ethics in the Life Sciences will be of interest to those planning and undertaking training activities in other areas. In asking how education and ethics are being made to matter in an emerging area of social unease, it will also be of interest to those with more general concerns about professional conduct.
    Keywords: biotechnology ; prevention ; ethics ; education ; Bioethics ; Biological warfare ; Biological Weapons Convention ; Biology ; Biosecurity ; China ; Dual-use technology ; bic Book Industry Communication::J Society & social sciences::JF Society & culture: general::JFM Ethical issues & debates::JFMG Ethical issues: scientific & technological developments ; bic Book Industry Communication::J Society & social sciences::JN Education
    Language: English
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    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: This book is intended to be a valuable addition to students, engineers, scientists, industrialists, consultants and others providing greater insight into wind tunnel designs and their enormous research potential. It is a compilation of works from world experts on subsonic and supersonic wind tunnel designs, applicable to a diverse range of disciplines. The book is organised in two sections. The first section comprises of three chapters on various aspects of stationary and portable subsonic wind tunnel designs, followed by one chapter on supersonic wind tunnel and the final chapter discusses a method to address unsteadiness effects of fan blade rotation. The second section contains four chapters regarding wind tunnel applications across a multitude of engineering fields including civil, mechanical, chemical and environmental engineering.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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    Taylor & Francis | Routledge
    Publication Date: 2024-04-07
    Description: Attending the World Economic Forum this past week, I was struck by two trends. The first was that brain research has emerged as a hot topic. Not only was brain science or brain health a new theme at the meeting, research on the brain emerged in discussions about next generation computing, global cooperation, and even models of economic development as well as being linked to mental health or mindfulness. In a meeting frequented largely by economists and business leaders, I was surprised by the number of non-scientists who have become enchanted by brain science. Clearly this is the era of the brain, with mental health now part of a much broader discussion.
    Keywords: policy ; society ; mental health ; Biology ; Epigenetics ; Genetics ; Genome ; Neuroscience ; Plastic ; Social policy ; thema EDItEUR::J Society and Social Sciences ; thema EDItEUR::M Medicine and Nursing
    Language: English
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    Coimbra University Press
    Publication Date: 2022-01-31
    Description: This work brings together various contributions from experts in very diverse areas of knowledge, to discuss the theme ‘Light’ from various points of view. The subjects gathered in this work come from the areas of Physics, Philosophy, Transcendence, Chemistry, Optics, Literature, History of Sciences, History, Geography, International Relations, Biology, Psychology, Art, Cinema and Photography, Medicine and Museology. The texts partially reflect the contents presented at the interdisciplinary colloquium ‘Visões da Luz’ held in October 2015, on the occasion of the International Year of Light 2015, under the aegis of III-UC and open to academia and society, to teachers of the Basic and Secondary Education.
    Keywords: Geography ; Chemistry ; Literature ; Geology ; Light ; Optics ; Biology ; History ; Physics
    Language: Portuguese
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    Logos Verlag Berlin | Logos Verlag Berlin
    Publication Date: 2024-04-05
    Description: The field of psychiatry changed dramatically in the latter half of the nineteenth century, largely by embracing science. The transformation was most evident in Germany, where many psychiatrists began to work concurrently in the clinic and the laboratory. Some researchers sought to discover brain correlates of mental illness, while others looked to experimental psychology for insights into mental dynamics. Featured here, are the lives and works of Emil Kraepelin - often considered the founder of modern scientific psychiatry, his teacher Bernhard Gudden, and his anatomist colleague Franz Nissl. The book describes scientific findings together with the methods used; it explains why diagnoses were then (and are still now) so difficult to make; it also explores mind-brain controversies. The Making of Modern Psychiatry will inform and delight mental health professionals as well as all persons curious about the origins of modern psychiatry.
    Keywords: Science ; Life Sciences ; Biology ; Psychology ; Technology & Engineering ; Agriculture ; thema EDItEUR::P Mathematics and Science::PS Biology, life sciences ; thema EDItEUR::J Society and Social Sciences::JM Psychology ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TV Agriculture and farming
    Language: English
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    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: This book reports the latest development and trends in the low Re number aerodynamics, transition from laminar to turbulence, unsteady low Reynolds number flows, experimental studies, numerical transition modelling, control of low Re number flows, and MAV wing aerodynamics. The contributors to each chapter are fluid mechanics and aerodynamics scientists and engineers with strong expertise in their respective fields. As a whole, the studies presented here reveal important new directions toward the realization of applications of MAV and wind turbine blades.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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    IntechOpen
    Publication Date: 2024-04-04
    Description: Spacecraft attitude maneuvers comply with Euler's moment equations, a set of three nonlinear, coupled differential equations. Nonlinearities complicate the mathematical treatment of the seemingly simple action of rotating, and these complications lead to a robust lineage of research. This book is meant for basic scientifically inclined readers, and commences with a chapter on the basics of spaceflight and leverages this remediation to reveal very advanced topics to new spaceflight enthusiasts. The topics learned from reading this text will prepare students and faculties to investigate interesting spaceflight problems in an era where cube satellites have made such investigations attainable by even small universities. It is the fondest hope of the editor and authors that readers enjoy this book.
    Keywords: Science ; Mechanics ; Aerodynamics ; bic Book Industry Communication::P Mathematics & science::PH Physics::PHD Classical mechanics::PHDF Fluid mechanics ; thema EDItEUR::P Mathematics and Science::PH Physics::PHD Classical mechanics::PHDF Physics: Fluid mechanics
    Language: English
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  • 39
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    Unknown
    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: Although great advances in computational methods have been made in recent years, wind tunnel tests remain essential for obtaining the full range of data required to guide detailed design decisions for various practical engineering problems. This book collects original and innovative research studies on recent applications in wind tunnel tests, exhibiting various investigation directions and providing a bird’s eye view on this broad subject area. It is composed of seven chapters that have been grouped in two major parts. The first part of the book (chapters 1–4) deals with wind tunnel technologies and devices. The second part (chapters 5–7) deals with the latest applications of wind tunnel testing. The text is addressed not only to researchers but also to professional engineers, engineering lecturers, and students seeking to gain better understanding of the current status of wind tunnels. Through its seven chapters, the reader will have an access to a wide range of works related to wind tunnel testing.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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  • 40
    facet.materialart.
    Unknown
    IntechOpen | IntechOpen
    Publication Date: 2024-04-11
    Description: Spacecraft attitude maneuvers comply with Euler's moment equations, a set of three nonlinear, coupled differential equations. Nonlinearities complicate the mathematical treatment of the seemingly simple action of rotating, and these complications lead to a robust lineage of research. This book is meant for basic scientifically inclined readers, and commences with a chapter on the basics of spaceflight and leverages this remediation to reveal very advanced topics to new spaceflight enthusiasts. The topics learned from reading this text will prepare students and faculties to investigate interesting spaceflight problems in an era where cube satellites have made such investigations attainable by even small universities. It is the fondest hope of the editor and authors that readers enjoy this book.
    Keywords: Aerodynamics ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TG Mechanical engineering and materials::TGM Materials science
    Language: English
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  • 41
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    transcript Verlag | transcript Verlag
    Publication Date: 2024-03-27
    Description: From self-help books and nootropics, to self-tracking and home health tests, to the tinkering with technology and biological particles - biohacking brings biology, medicine, and the material foundation of life into the sphere of »do-it-yourself«. This trend has the potential to fundamentally change people's relationship with their bodies and biology but it also creates new cultural narratives of responsibility, authority, and differentiation. Covering a broad range of examples, this book explores practices and representations of biohacking in popular culture, discussing their ambiguous position between empowerment and requirement, promise and prescription.
    Keywords: Culture ; Representation ; Biology ; Medicine ; Biocultures ; Biohacking ; Biotechnology ; Cultural Narratives ; DIY ; America ; Body ; Biopolitics ; American Studies ; Life Sciences ; Cultural Studies ; thema EDItEUR::D Biography, Literature and Literary studies::DS Literature: history and criticism::DSB Literary studies: general ; thema EDItEUR::J Society and Social Sciences::JP Politics and government::JPA Political science and theory ; thema EDItEUR::M Medicine and Nursing::MB Medicine: general issues
    Language: English
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  • 42
    Publication Date: 2023-07-27
    Description: Table Bay, South Africa, is a typical headland-bay system with a shoreline that can be described by a logarithmic spiral. A peculiarity and unique feature of Table Bay is the juxtaposition of Robben Island opposite its headland. As a consequence, the bathymetry defines an ellipsoidal basin which was postulated to potentially resonate in the form of long-period standing waves (seiches). One aim of this study, therefore, was to investigate whether any evidence for such resonant oscillations could be detected in the geomorphology and sediment distribution patterns. Indeed, the ellipsoidal shape of the basin can be framed by two converging log-spirals with their centres located opposite each other, one off Robben Island and the other on the Cape Town side of the bay. The so-called apex line, which divides the two spirals into equal parts is aligned SW–NE, i.e. more or less parallel to the direction of ocean wave propagation. The distribution patterns of all sedimentary parameters were found to be characterised by a strikingly similar trend to either side of the apex line. This supports the hypothesis that the basin of Table Bay appears to resonate in the form of a mode 1 standing wave, with the node positioned above the apex line in the centre of the bay. The maximum period of such a standing wave was calculated to be around 37 min. The study demonstrates that large-scale sediment distribution patterns can reveal the existence of specific hydrodynamic processes in coastal embayments. It is recommended that this phenomenon be investigated in greater detail aimed at verifying the existence of resonant oscillations in Table Bay and, in the event, at establishing its precise nature and trigger mechanism.
    Description: Council for Geoscience and CSIR South Africa
    Description: Senckenberg Gesellschaft für Naturforschung (SGN) (3507)
    Keywords: ddc:551.46 ; Oceanography ; South Africa ; Table Bay ; ellipsoidal shape ; sediment distribution ; seiches
    Language: English
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  • 43
    Publication Date: 2023-07-27
    Description: Sea-level rise represents a severe hazard for populations living within low-elevation coastal zones and is already largely affecting coastal communities worldwide. As sea level continues to rise following unabated greenhouse gas emissions, the exposure of coastal communities to inundation and erosion will increase exponentially. These impacts will be further magnified under extreme storm conditions. In this paper, we focus on one of the most valuable coastal real estate markets globally (Palm Beach, FL). We use XBeach, an open-source hydro and morphodynamic model, to assess the impact of a major tropical cyclone (Hurricane Matthew, 2016) under three different sea-level scenarios. The first scenario (modern sea level) serves as a baseline against which other model runs are evaluated. The other two runs use different 2100 sea-level projections, localized to the study site: (i) IPCC RCP 8.5 (0.83 m by 2100) and (ii) same as (i), but including enhanced Antarctic ice loss (1.62 m by 2100). Our results show that the effective doubling of future sea level under heightened Antarctic ice loss amplifies flow velocity and wave height, leading to a 46% increase in eroded beach volume and the overtopping of coastal protection structures. This further exacerbates the vulnerability of coastal properties on the island, leading to significant increases in parcel inundation.
    Description: Universität Bremen (1013)
    Description: https://github.com/pboyden/Palm_Beach_XBeach
    Keywords: ddc:551.46 ; Oceanography ; tropical cyclones ; coastal areas ; sea level scenarios ; hydrodynamic modeling ; morphodynamic modeling
    Language: English
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  • 44
    Electronic Resource
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    Springer
    Pure and applied geophysics 117 (1979), S. 943-957 
    ISSN: 1420-9136
    Keywords: Oceanography ; Dish pan experiments ; Dynamics of oceans ; Laboratory modellings of ocean currents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The Loop Current of the Gulf of Mexico is simulated in the laboratory. A circular tank is filled with water and is placed off-center on a rotating table and the flow field is generated by injecting and withdrawing water at two openings on the wall. The free surface becomes parabolic due to balance of gravitational and centrifugal forces, simulating the latitudinal change of the Coriolis parameter (β-effect) in the ocean. The flow characteristics depend on the influx and the rate of rotation and can be classified according to non-dimensional parameters (Rossby, Ekman and Froude numbers denoted byR 0,E andF, respectively). When the influx is small and the rotation rate is large (smallR 0,E andF) the flow will be almost linear, and the fluid flows along the side-wall boundary layer under constraint of the β-effect. For a very large influx (largeR 0 andE) inertial forces become very large compared to the Coriolis force and the flow behaves like a potential flow. The flow studied had characteristics between these two extreme cases and hasR 0 andF similar to the Gulf circulation, though similarity inE is ambiguous. Photographs of the flow indicate that the inflow penetrates further into the interior when the rotation rate is increased while the influx is kept constant. The numerical analysis of the non-linear vorticity equation confirms this for the parameters corresponding to the experiment. In addition, the photographs reveal eddies embedded on both sides of the main stream, particularly near the inflow region. These eddies are intensified and become uniform in size as the influx increases. It is pointed out that such eddies were actually observed near the Loop Current north of the Yucatan Straits.
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  • 45
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    Biopolymers 18 (1979), S. 35-45 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dielectric measurements have been carried out on partially hydrated collagen in the frequency ranges 100 kHz-5 MHz, 100 MHz-1 GHz, and 8-23 GHz. In the low-frequency range, a dispersion was observed around 100 kHz which results from inhomogeneous conductivity of the samples. A dielectric relaxation was observed aroud 0.3 GHz using time-domain-spectroscopy techniques. This relaxation can be considered to originate from mobile side chains. Microwave measurements indicate that the water relaxation may extend into the 10-GHz region. An apparent discrepancy between the main water relaxation time and the average rotational correlation time of water as measured by nmr line widths was resolved by the assumption that a fraction of the water molecules is bound to the collagen with residence times on the order of 10-6 sec, whereas the remainder of the water is only weakly bound and exhibits rotational rates on the order of 10-10 sec.
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  • 46
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    Biopolymers 18 (1979), S. 83-100 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A model for the time dependence of DNA conformational state probabilities is formulated in the form of first-order differential equations. This model is applied to investigate the renaturation and denaturation rates for T2 and T7 DNA as reported in the series of experiments by Record and Zimm. Qualitative agreement is found in denaturation and for series of renaturation experiments with the same initial condition. However, partial agreement with series of renaturation experiments having the same final condition is obtained only by including an initial bimolecular step with properly matched pairs of strands. Comparison of all experiments with the calculated rates yields 5 × 104 min-1 as the step rate for melting a single base pair.
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  • 47
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conventionally protected oligopeptides of the two homologous series Boc-(L-Ile)n-OMe and Boc-(D-aIle)n-OMe (n = 2-6) were synthesized in a standard stepwise fashion and their uv and CD spectra in 2,2,2-trifluoroethanol, and solid-state ir spectra were investigated. In addition, two oligomeric products derived from the NCAs of L-isoleucine and of D-allo-isoleucine and having a DP of 20 and 12, respectively, were studied in the solid state by x-ray and ir. No substantial differences between the properties of the diastereomeric oligomers in the solid state were noticed, a β-structure being very likely at least for the Boc-protected hexapeptides and the higher oligomers. In contrast, differences were observed between the spectroscopic properties of the diastereomeric oligopeptides, and especially of the hexapeptides, in trifluoroethanol solution. The different properties of the hexapeptides in solution were related to the existence, in the case of Boc-(L-Ile)6-OMe, of soluble molecular aggregates in which the peptide chains assume the β-conformation. These results provide an additional example of the influence of the configuration of asymmetric carbon atoms of the side chains on the conformational properties of peptide molecules in solution.
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  • 48
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    Biopolymers 18 (1979), S. 285-297 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 1H-nmr chemical shifts and the spin-spin coupling constants of the common amino acid residues were measured in solutions of the linear tetrapeptides H-Gly-Gly-X-L-Ala-OH in D2O and H2O, the influence of X on the nmr parameters of the neighboring residues Gly 2 and Ala 4 was investigated. The titration parameters for the side chains of Asp, Glu, Lys, Tyr, and His were determined. The pKa values obtained in D2O, with the use of pH-meter readings with a combination glass electrode uncorrected for istope effects, were 0.06 pH units higher in the acidic range and 0.10 pH units higher in the basic range than the corresponding pKa values in H2O. This suggests that the present data are suitable “random-coil” 1H-nmr parameters for conformational studies of polypeptide chains in D2O and H2O solutions.
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  • 49
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    Biopolymers 18 (1979), S. 299-311 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper shows that backbone amide proton titration shifts in polypeptide chains are a very sensitive manifestation of intramolecular hydrogen bonding between carboxylate groups and backbone amide protons. The population of specific hydrogen-bonded structures in the ensemble of species that constitutes the conformation of a flexible nonglobular linear peptide can be determined from the extent of the titration shifts. As an illustration, an investigation of the molecular conformation of the linear peptide H-Gly-Gly-L-Glu-L-Ala-OH is described. The proposed use of amide proton titration shifts for investigating polypeptide conformation is based on 360-MHz 1H-nmr studies of selected linear oligopeptides in H2O solutions. It was found that only a very limited number of amide protons in a polypeptide chain show sizable intrinsic intration shifts arising from through-bond interactions with ionizable groups. These are the amide proton of the C-terminal amino acid residue, the amide protons of Asp and the residues following Asp, and possibly the amide proton of the residue next to the N-terminus. Since the intrinsic titration shifts are upfield, the downfield titration shifts arising from conformation-dependent through-space interactions, in particular hydrogen bonding between the amide protons and carboxylate groups, can readily be identified.
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  • 50
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    Biopolymers 18 (1979), S. 359-372 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We present a method that can reduce conformational energy calculations for an arbitrary peptide consisting of n residues (n-peptide) to the complexity of a computation for (Gly)n. This reduction, and the concomitant savings in computer time, is accomplished by replacing all side chains, as well as the backbone CαHα and CαH2α groups, by “interaction centers.” The backbone CONH group is left intact in order to preserve its directional character. The interaction centers “see” each other, and the atoms of the CONH group via Boltzmann and space-averaged effective center-center and center-atom potentials, respectively. This averaged-interaction method is tested on the repeat tetra-, penta-, and hexapeptides of elastin, Val-Pro-Gly-Gly (VPGG), Val-Pro-Gly-Val-Gly (VPGVP), and Ala-Pro-Gly-Val-Gly-Val (APGVGV), using the stereoalphabet strategy for the energy calculations. The excellent qualitative and quantitative agreement we obtain with both full atom-atom calculations and extensive nmr data, coupled with the order-of-magnitude reduction in computer time, augurs well for the potential usefulness of the method.
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  • 51
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    Biopolymers 18 (1979), S. 393-409 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Linear response theory in the decorrelation or random-phase approximation is used to calculate the absorption and CD spectra of model helical polymers, including single-stranded polyadenylic acid. The method, which makes use of infinite polymer selection rules for the linear response tensor, has the advantages that (1) only a few three-dimensional matrices need be inverted; (2) spectral band shapes of the polymer arise naturally from those of the monomer, as well as from the geometry-dependent interactions in the helix; and (3) the spectral dependence on geometrical factors of the helix is made transparent. It is found that the structure of the polymer CD spectrum depends critically on monomer bandshape. An asymmetric CD spectrum, similar to some experimental spectra, arises from either a Gaussian or a composite monomer band. Single-stranded polyadenylic acid spectra are sensitive to helix geometry in the region 200-240 nm, in reasonable agreement with experimental spectra. This sensitivity arises from the 207-nm monomer transition, and the results suggest that this region of the spectrum should be more fully exploited as a tool for helix geometry studies.
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  • 52
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: C24H34N2O9, orthorhombic, P212121; a = 39.432 (10), b = 14.061 (5), c = 4.850 (2) Å, M = 494 a.m.u., Z = 4, Dm = 1.22 g cm-3, Dx = 1.22 g cm-3, R = 0.13 for 1205 observed reflections after refinement with isotropic thermal factors. The urethane and amide bonds are in the trans configuration, as well as all the ester groups. The ϕ and ψ angles of the L-glutamyl residues fall in the β-structure region of the Ramachandran's plot; the molecule is rather flat with the amide plane almost parallel to the c axis along which two hydrogen bonds hold the molecules together to form long rows in a “parallel pleated-sheet” fashion.
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  • 53
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The far-ir absorption spectrum of lysozyme was measured at room and liquid-nitrogen temperatures. Dried layers of single crystals of tetragonal lysozyme chloride with a diameter of 100-300 μm were grown on a silicon plate. Such single-crystalline samples were considered to have the following advantages in obtaining far-ir spectra: (1) surface scattering is reduced, (2) the protein molecules are closely packed, and (3) air-drying of the crystals reduces the number of water molecules without considerably changing the original configuration. The spectrum obtained consisted of a strong background absorption and a number of absorption peaks that were not clearly observed with the sample in the form of lyophilized powder. The peaks were ascribed to various delocalized vibrations of the main and side chains in the molecule. The peaks were also compared with the positions of Raman lines. The uniform background was assigned to the water molecules remaining in the crystals.
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  • 54
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    Biopolymers 18 (1979), S. 553-569 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The duplex-to-strand transition of the self-complementary sequence dG-dC-dG-dC has been probed at the exchangeable and nonexchangeable protons and backbone phosphates by high-resolution nmr spectroscopy. The Watson-Crick imino and amino hydrogen-bonded protons, as well as the exposed amino protons, could be followed through the duplex-to-strand transition and provide information on base-pair stability at the tetranucleotide duplex level. The magnitudes of the experimental upfield nonexchangeable base-proton chemical shifts on duplex formation are consistent with calculations based on base-pair overlap geometries of the B-DNA type. The variation of the 31P chemical shifts in dG-dC-dG-dC with temperature appear to monitor changes in the ω,ω′ rotation angles about the O—P bonds in the postmelting transition temperature region. The complex formed between the antitumor anthracycline antibiotic daunomycin and the dG-dC-dG-dC duplex was probed at the nucleic acid and the antibiotic resonances as a function of temperature. The experimental complexation shifts of the observable daunomycin resonances have put constraints on possible overlap geometries between the intercalating anthracycline ring and adjacent base pairs.
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  • 55
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conformational analyses of cyclic tetrapeptides consisting of alternating cis and trans peptide units have been made using contact criteria and energy calculations. This study has been restricted to those structures having a symmetry element in the backbone ring, such as a twofold axis (d) or a center of inversion (i). There are five main results. (1) There are two distinct types of conformations, which are stereochemically favorable corresponding to each of twofold and inversion-symmetrical structures, designated as d1, d2 (for twofold symmetrical) and i1, i2 (for inversion-symmetrical). Among these, the i1 type has the lowest energy when glycyl residues occur at all four α-carbon atoms. (2) With the glycyl residue at all four α-carbon atoms, methyl substitution at the cis peptide nitrogen atoms is possible in all the four types, whereas the substitution at trans peptide nitrogen atoms is possible only for the i1 type. Thus only in the i1 type can all the nitrogen atoms be methylated simultaneously. The conformation of the molecule in the crystal structure of cyclotetrasarcosyl belongs to the i1 type. (3) When alanyl residues occur at all four α-carbon atoms, the possible symmetrical type is dependent on the enantiomorphic form and the actual sequence of the alanyl residues. (4) The methyl substitution at peptide nitrogen atoms for cyclic tetrapeptides having alanyl residues causes more stereochemical restriction in the allowed conformations than with glycyl residues. (5) The prolyl residue can be incorporated favorably at the cis-trans junction of both d and i types of structures. The results of the present study are compared with the data on cyclic tetrapeptides available from the crystal structure and nmr studies. The results show an overall agreement both regarding the type of symmetry and the conformational parameters.
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  • 56
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Based on equilibrium binding studies, as well as on kinetic investigations, two types of interactions of Cu2+ ions with native DNA at low ionic strength could be characterized, namely, a nondenaturing and a denaturing complex formation. During a fast nondenaturing complex formation at low relative ligand concentrations and at low temperatures, different binding sites at the DNA bases become occupied by the metal ions. This type of interaction includes chelate formation of Cu2+ ions with atoms N(7) of purine bases and the oxygens of the corresponding phosphate groups, chelation between atoms N(7) and O of C(6) of the guanine bases, as well as the formation of specific intestrand crosslink complexes at adjacent G°C pairs of the sequence dGpC. CD spectra of the resulting nondenatured complex (DNA-Cu2+)nat may be interpreted in terms of a conformational change of DNA from the B-form to a C-like form on ligand binding. A slow cooperative denaturing complex formation occurs at increased copper concentrations and/or at increased temperatures. The uv absorption and CD spectra of the resulting complex, (DNA-Cu2+)denat, indicate DNA denaturation during this type of interaction. Such a conclusion is confirmed by microcalorimetric measurements, which show that the reaction consumes nearly the same amount of heat as acid denaturation of DNA.From these and the kinetic results, the following mechanism for the denaturing action of the ligands is suggested: binding of Cu2+ ions to atoms N(3) of the cytosine bases takes place when the cytosines come to the outside of the double helix as a result of statistical fluctuations. After the completion of the binding process, the bases cannot return to their initial positions, and thus local denaturation at the G·C pairs is brought about. The probability of the necessary fluctuations occurring is increased by chelation of Cu2+ ions between atoms N(7) and O of C(6) of the guanine bases during nondenaturing complex formation, which loosens one of the hydrogen bonds within the G·C pairs, as well as by raising the temperature. The implications of the new binding model, which comprises both the sequence-specific interstand crosslinks and the described mechanism of denaturing complex formation, are discussed and some predictions are made. The model is also used to explain the different renaturation properties of the denatured complexes of Cu2+, Cd2+, and Zn2+ ions with DNA.In temperature-jump experiments with the nondenatured complex (DNA-Cu2+)nat, a specific kinetic effect is observed, namely, the appearance of a lag in the response to the perturbation. The resulting sigmoidal shape of the kinetic curves is considered to be a consequence of the necessity of disrupting a certain number of the crosslinks existing in the nondenatured complex before the local unwinding of the binding regions (a main step of denaturing complex formation) may proceed.
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  • 57
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    Biopolymers 18 (1979), S. 931-938 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A new analysis has been made on studies of the influence of imino acid content on the changes of collagen thermal stability (tm). It is shown that, for the interstitial vertebrate collagens, there is a strict regularity in the changes of tm depending on hydroxyproline content. No correlation is observed between tm and proline content. Also, no correlation between tm and hydroxyproline content is observed for invertebrate and basement membrane collagens. On the basis of the reported data, the dependence of tm on hydroxyproline content is considered to be not a correlation between tm and the total content of hydroxyproline, but only as the correlation between tm and the content of hydroxyproline occurring at the third position in the sequence (Gly-R2-R3)n. The results agree with the idea that the influence exerted by proline and hydroxyproline on the stabilization of the triple helix of collagen is different.
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  • 58
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformation and conformational transitions of poly(His-Ala-Glu) have been investigated by ir, nmr, and CD measurements. The results obtained - as well as the results of our previous investigations by potentiometric titration and hydrodynamic techniques [Goren et al., Biopolymers (1977) 16, 1541-1555] - indicate that when dissolved in water, the co-polymer assumes a disordered conformation. On changing the pH of the solution, the states of ionization of the side-chain imidazole and carboxyl groups change in the same manner as in low-molecular-weight model compounds. Concomitantly, the overall shape of the macromolecule is altered, while the conformation of the polypeptide backbone changes from one disordered state to another but never assumes a regular form. In water/methanol and water/trifluoroethanol mixtures, transitions from a disordered state to the α-helix conformation were observed on increasing the alcohol content of the system. The conformational transitions followed pathways which differ from one another according to the experimental conditions employed. Conformational landmarks (intermediates) have been identified along these pathways.
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  • 59
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    Biopolymers 18 (1979), S. 1023-1026 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 60
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    Biopolymers 18 (1979), S. 1821-1828 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The decrease in the limiting viscosity number [η] with temperature T for hyaluronic acid in nonalkaline solution and chondroitin 4-sulfate in neutral and alkaline solutions may be expressed in terms of the temperature coefficient of the Kratky-Porod persistence length a: d ln a/dT = -0.0040 (±0.0005). The result, while numerically somewhat smaller, resembles qualitatively that of cellulose derivatives. As in the latter case, standard conformational calculations underestimate the coefficient, which may be due to neglect of random occurrence of local conformational features of higher energy. In alkaline solution, large decreases in [η] of hyaluronic acid are accompanied by a positive temperature coefficient of [η]. This temperature effect is interpreted as an endothermal shift from the alkaline, low [η] form of the polymer to the neutral, high [η] form with increasing temperature.
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  • 61
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    Biopolymers 18 (1979), S. 1809-1820 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have considered whether or not the tertiary structure of a biomolecule is the same in a crystal (or an oriented film) as it is in solution. A methodology has been developed for comparing polarized absorption spectra obtained from a solid-state sample with those obtained from an oriented solute to further resolve this question. An electric dichroism instrument built in our laboratory was used to measure the solution dichroism signal which, along with the ordinary solution uv absorption spectra, yields polarized absorption spectra in the directions parallel and perpendicular to the applied electric field. These were then compared to polarized absorption data from oriented films of nucleic acids to determine whether the two sets of data could be rotated into coincidence. This rotation was accomplished using a computer program based on a nonlinear programming method. Four nucleic acids were studied and the film and solution data for three of these were found to be equivalent, requiring rotation through an angle of 3°-20°, depending on film humidity, to bring them into coincidence. For the fourth sample we were unable, perhaps because of signal-to-noise ratio limitations, to find a correlation. Flow dichroism and electric dichroism data were also found to be quite similar. Thus it is clear that the induced dipole moment is along the helical axis and that the physical, hydrodynamical, and electrical axes of the nucleic acid molecules are equivalent.
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  • 62
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Chemistry and Pharmacology
    Notes: The effect of enzyme-inhibitor complex formation on the hydration properties of the macromolecular moiety was investigated on the model system of α-chymotrypsin and its Ser-195 tosyl derivative. The primary (A-shell) hydration of the native and modified enzyme was compared by sorption measurements. The secondary (B-shell) hydration water was investigated by differential scanning calorimetry. Tosylation is known to induce pronounced conformational changes in the chymotrypsin molecule. These structural modifications have the following effects on the hydration of the native enzyme.The water binding capacity of the protein surface is significantly increased, as shown by both the calorimetric and the sorption results. The amount of unfreezable water of primary hydration is increased by 50 mol H2O/mol chymotrypsin.The heats (ΔH) and entropies (ΔS) of the interaction of water with chymotrypsin are strongly reduced in the modified enzyme. This effect is interpretable by a reduction of the H bonding potential of the protein surface. Parallel to this decrease in δH, the heats of fusion of the secondary hydration water (Qfus) are significantly increased by tosylation (Qfus = 256.2 ± 7.8 and 294.2 ± 4.8 J g-1 H2O for the native and the tosylated enzyme, respectively). This increase in Qfus reflects an increase in the extent of H bonding in the B-shell hydration sphere.These changes in the hydration of the native enzyme, associated with the reaction: native chymotrypsin → tosylchymotrypsin, are interpreted by cooperative phase transitions of water molecules in the primary and secondary hydration water. One of these transitions was found to exhibit a significant, linear enthalpy-entropy compensation effect. The compensation temperature \documentclass{article}\pagestyle{empty}\begin{document}$ \hat{\beta} $\end{document} is 290.7 ± 2.8°K. This \documentclass{article}\pagestyle{empty}\begin{document}$ \hat{\beta} $\end{document} value agrees well with compensation temperatures reported in the literature for a series of biochemical reactions in aqueous solution (250-320° K). This agreement in \documentclass{article}\pagestyle{empty}\begin{document}$ \hat{\beta} $\end{document} may point to a common source of both compensation phenomena.
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    Biopolymers 18 (1979), S. 1829-1830 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Biopolymers 18 (1979), S. 1831-1833 
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Chemistry and Pharmacology
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    Biopolymers 18 (1979) 
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    Keywords: Chemistry ; Polymer and Materials Science
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  • 66
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    Biopolymers 18 (1979), S. 1835-1848 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformations of melanostatin have been studied experimentally using CD spectroscopy and via calculations. In aqueous solution and 2,2,2-trifluoroethanol (TFE) there is no evidence that monomers of the tripeptide exist in an ordered (β-bend) structure. In water and TFE solutions (3-6 × 10-4M) the neutral molecules aggregate very slowly, taking about 3 days to attain equilibrium at room temperature. At equivalent concentrations in TFE, although not in water, the cationic molecules also slowly aggregate, although to a lesser extent. Calculations using rotational isomeric state theory give the most probable unperturbed end-to-end distance of the molecule at 9.3 ± 0.1 Å and indicate that a vast majority of the molecules exist in some extended conformation, end-to-end distance ≥6 Å. Only 0.4% of the molecules are calculated to have O…H separations compatible with a β-bend structure. An intramolecular hydrogen bond must have an energy at least 2 kcal/mol lower than that of an intermolecular hydrogen bond to solvent if a β-bend is to be experimentally observable.
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  • 67
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    Topics: Chemistry and Pharmacology
    Notes: Nps-[Glu(OBzl)]6-NHEt has been prepared by coupling Nps-[Glu(OBzl)]2-OH with HCl,H-[Glu(OBzl)]4-NHEt by means of dicyclohexylcarbodiimide. The ir spectra of its nujol mull show that the hexapeptide has the β-structure of antiparallel chains. When it is dissolved in dioxane or ethylene dichloride, the hexapeptide consists of a mixture of the β-form and the solvated σ-form, but the β-form can exist only above a certain critical concentration. The critical concentration is about 0.4g dl-1 in dioxane and 0.08g dl-1 in ethylene dichloride, and the content of β-form increases with increasing concentration above it. The CD of the dioxane and ethylene dichloride solutions shows concentration dependence in visible and uv regions.
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    Biopolymers 18 (1979), S. 2115-2126 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Light scattering from the solutions of Nps-[Glu(OBzl)]6-NHEt in dioxane or ethylene dichloride has been measured at different concentrations, and a critical concentration of intermolecular association is found to exist, which is equal to the critical concentration of β-form formation. The Debye plot of light scattering leads to the molecular weight of aggregates at the critical concentration, which corresponds to an aggregation number 15 in dioxane and 53 in ethylene dichloride. In the latter solvent the aggregates further associate into a larger aggregate consisting of 330 molecules when the concentration is increased beyond the critical concentration. The content of β-form, which is a measure of number of hydrogen bonds, is derived from the ir data previously obtained. The results on the modes of intermolecular association and hydrogen bonding lead to possible structures of aggregates formed by both hydrogen bonds and other nonbonding side-chain interactions.
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  • 69
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    Topics: Chemistry and Pharmacology
    Notes: Low shear viscosities have been determined for a 1 mg/ml poly(L-lysine) solution as a function of added salt concentration in the region of the previously reported ordinary-extraordinary phase transition. The measured viscosities indicate that the polyions are far from completely extended at the transition. Estimates of the longest internal relaxation time for an equivalent free-draining Rouse-Zimm chain give τ ≃ 10-5 sec, similar to that of the rapid, angle-independent component previously observed in the dynamic light-scattering correlation function at the transition. An unusual peak and valley are observed in the curve of [η]0 versus [NaBr] in the transition region. Possible interpretations of these features, and their bearing on the nature of the extraordinary phase, are discussed.
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  • 70
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    Keywords: Chemistry ; Polymer and Materials Science
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    Topics: Chemistry and Pharmacology
    Notes: The crystal structure of the valinomycin analog, cyclo-[(-D-Val-Hyi-Val-D-Hyi-)3-] (meso-valinomycin, C60H102N6O18) has been determined by direct x-ray diffraction procedures. The crystals are triclinic, space group P1, number of molecules per unit cell Z = 1, and cell parameters a = 11.831, b = 13.815, c = 14.889 Å, α = 109.54°, β = 116.10°, γ = 98.89°. The atomic coordinates for the C,N,O atoms were refined in the anisotropic thermal motion approximation and for the H atoms in the isotropic approximation to R = 0.07.The structure is centrosymmetric and has a threefold axis of pseudosymmetry. The depsipeptide chain is in the form of a bracelet stabilized by six identical intramolecular 4 → 1 hydrogen bonds between the amide C=O and NH groups. The ester carbonyls are oriented towards the symmetry axis, their O atoms forming an ellipsoidal molecular cavity. The isopropyl side chains are located on the molecular periphery. The structure found differs considerably from the conformation of the crystalline naturally occurring antibiotic, valinomycin, but completely resembles that of valinomycin and meso-valinomycin in nonpolar solvents. In the crystal, meso-valinomycin molecules form stacks. The molecular cavities situated in the stacks one above the other along the pseudo-C3 axis form a continuous channel, the internal surface of which is lined by O atoms. The possible conformations of depsipeptides of the valinomycin series and their mode of action in membranes are discussed in the light of the data obtained.
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    Biopolymers 18 (1979), S. 2353-2356 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) Biophys. Chem. 1, 279-291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gradinets at low and high rotor speeds show G DNA sedimenting from 1.8 to 0.7 times as fast as T2 DNA. Experimental measurements indicate that the sedimentation coefficient of G DNA decreases with increasing rotor speed about as fast as predicted by theory.
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  • 72
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have examined the NH stretching frequencies of N-acetyl-N′-methyl-L-alanineamide (blocked Ala), N-acetyl-N′-methylglycineamide (block Gly), and N-acetyl-N′-methyl-L-leucineamide (blocked Leu) in chloroform using irspectroscopy. Their spectrum of blocked Leu in carbon tetrachloride was also obtained. A major absorption band at 3450 cm-1 is attributed to the unperturbed NH stretching frequency. Another major band at 3437 cm-1 (for Ala) or 3432 cm-1 (for Leu) is attributed to conformations in which the NH stretching frequency is perturbed by the spatial proximity of the Cβ atom. An absorption band between 3300 and 3370 cm-1, which has in the past been assigned to the intramolecular hydrogen-bonded NH in the C7eq conformation, was found to be concentration dependent and could not be observed below 5 × 10-4M in chloroform; thus we find no evidence for a strongly hydrogen-bonded NH in the C7eq conformation in chloroform. An absorption band at 3416 cm-1 was observed in chloroform solutions of blocked Gly, and a similar absorption appeared as a shoulder on the 3437- and 3432-cm-1 bands of blocked Ala and blocked Leu, respectively, in the same solvent. These bands, occurring near 3416 cm-1, may be assigned to extended (C5) conformations [Avignon et al., Biopolymers 8, 69 (1969)]. In CCl4 the spectrum of blocked Leu remained concentration dependent below 2.8 × 10-4M, with the 3300-3370-cm-1 band progressively weakening and shifting to higher frequencies on dilution from higher concentrations. Analysis of the spectra indicates that there is considerable flexibility in the blocked single residues, in agreement with the results of conformational energy calculations.
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    Biopolymers 18 (1979), S. 2523-2535 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Type I collagen fibrillogenesis in vitro has been studied by laser light scattering, and the results indicate that initiation of aggregation involves at least two steps. Step I of aggregation involves no change in the intensity of scattered light at an angle of 90° and is accompanied by a decrease in the diffusion coefficient. Step II is characterized by an increased intensity of scattered light and decreased diffusion coefficients. Theoretical calculations using the Stokes-Einstein equation for the translational diffusion coefficient and the Perrin equation for the frictional coefficient of a prolate ellipsoid indicate that the step I aggregates are 4D staggered linear dimers and trimers 570 and 845 nm long, whereas step II aggregates are greater than 950 nm in length. These dimensions are similar to those previously reported based on physicochemical measurements and electron microscopy. It is proposed that the rate and extent of fibrillogenesis in vitro is controlled by the concentration of the linear aggregates and that the effects of temperature and collagen concentration on fibrillogenesis previously observed are qualitatively explained in terms of their effects on the concentration of these aggregates.
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    Biopolymers 18 (1979), S. 2537-2547 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The method hitherto used for estimating the electrostatic term in empirical intramolecular calculations of stable conformations of biologically important molecules and macromolecules and intermolecular calculations of molecular associations or packing energy in molecular crystals had been analyzed. It has been shown that the contribution of atomic hybridization moments is omitted in the calculation of electrostatic interactions from net atomic charges localized on nuclei which have been determined by standard quantum-chemical methods. This contribution plays an important part in determining electrostatic interactions, mainly in molecules containing atoms with lone pairs. Simultaneously, a modified method for calculating the electrostatic term comprising the interaction of the lone pairs, which are represented by atomic hybridization moments, has been proposed. The relationship between the atomic hybridization moment and the bond angle has been expressed for some typical configurations occurring in biologically important molecules. Finally, this new approach is illustrated by results of the conformational analysis of some model compounds for biomolecules and compared with the approach used so far for the estimation of the electrostatic interaction in empirical methods of calculation of the intra- and intermolecular energy.
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    Biopolymers 18 (1979), S. 2549-2567 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Intensity fluctuations of laser light scattered from filamentous viruses Pf1 [length L (Å) × diameter d (Å) = 20,000 × 90], M13 (9000 × 90), potato virus X (5150 × 130), and tobacco mosaic virus (3000 × 180) in sucrose density gradients were measured with a photon correlation spectrometer over a range of scattering angles from 15° to 120°. The experimental data can be approximated by two exponential decays, “slow” and “fast.” The slow decay rate constant ts-1 corresponds to the translational diffusion D of the virus, i.e., ts-1 = K2D, where K is the magnitude of the scattering vector. The amplitude of the slow component, i.e., translational diffusion, remains greater than that of the fast component, even at high KL. The fast decay rate constant tf-1 is also proportional to K2 for viruses such as Pf1, M13, and even potato virus X. In the companion paper, we shall attribute the amplitude enhancement of the translational diffusion to the coupling of its anisotropy to the rotational diffusion modes. In order to explain the excessive decay rates in the fast component, we need to consider the bending mode of rodlike viruses, especially in the longer viruses such as M13 and Pf1, in addition to the usually expected rotational diffusion modes.
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    Biopolymers 18 (1979), S. 2569-2588 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We have compared four theoretical effects of rodlike macromolecules with the fast components, i.e., components other than translational diffusion, of our experimental data, which are presented as amplitude autocorrelation functions of electric field scattered from dilute solutions of monodisperse rodlike viruses with lengths from 3300 Å for tobacco mosaic virus to 20,000 Å for Pf1. The four effects are (1) the optic anisotropy treated by Aragón and Pecora, (2) coupled translational-rotational diffusion due to anisotropy in translational mobility recently reformulated by Gierke, (3) anisotropic rotational diffusion with respect to the direction of translational displacement first discussed by Berne and Pecora, and (4) the bending mode of a rod by Fujime and Maruyama. We show that both the first and second effects are required to explain the enhancement of amplitude of the translational diffusion at the expense of fast components. The experimental decay rates of the fast component exceed that of the rotational diffusions. In order to explain the excessive decay rate in the fast component, we need to include a minute amount (∼1%) of bending mode of rodlike viruses, especially in longer viruses such as M13 and Pf1.
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    Biopolymers 18 (1979), S. 2607-2623 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The previous report that poly(L-glutamic acid) exhibits doubled resonances in the helix-coil transition region by either proton or carbon-13 nmr resolves the question of whether or not this behavior is limited to uncharged polypeptides in organic solvents, as had been previously thought. In the present work, we show that the underlying principle causing this anomalous double-peak behavior is due to molecular-weight polydispersity of the sample. The molecular-weight range in which this phenomenon is observed is largely dependent on the values of σ, the nucleation or cooperativity factor. The principles developed are shown to encompass all classes of polypeptides in a very natural way and to explain the key experimental data in the literature.
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    Biopolymers 18 (1979), S. 2589-2606 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Empirical conformational energy calculations with the use of ECEPP energy functions have been carried out for linear dipeptides H-X-L-Pro-OH, with X = Gly, L-Ala, D-Ala, L-Leu, D-Leu, L-Phe, and D-Phe, in different states of protonation of the end groups. The results of these calculations are compared with the previously reported experimental equilibrium populations for the cis and trans isomers of the X-Pro bond in the different species. For all the protonation states of the seven dipeptides, the calculated nonbonded interactions and the conformational entropy term lead to a preference of the trans forms over the cis isomers by at least 1 kcal/mol. The electrostatic interactions stabilize the cis conformations in all species except the cationic forms of the D,L-peptides, and it could further be shown that only the carbonyl group of X and the two end groups contribute significantly to the total electrostatic energy. One of the principal results of the experimental studies, i.e., the occurrence of 5-15% cis-proline in all the peptides with an uncharged C-terminus, was corroborated by our investigation of the cationic species. A detailed assessment of the electrostatic contribution to the total energy of the different conformations of H-Gly-L-Pro-OH indicates that the standard ECEPP parameters tend to overestimate the electrostatic interactions in aqueous solutions of the X-Pro dipeptides.
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    Biopolymers 18 (1979) 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Biopolymers 18 (1979), S. 2625-2643 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The secondary structure of the lac repressor protein proposed by Chou et al. has been modified to include the recent revisions in sequence. In addition to the Chou and Fasman method, five other methods were used; they include those of (1) Lim, (2) Ptitsyn and Finkelstein, (3) Burgess et al., (4) Bunting et al., and (5) Wu and Kabat. Any two individual methods gave results differing sharply from one another. Three or more methods were in agreement for 91, 39, and 126 residues in helix, in β, and in combined coil plus turn conformations, respectively; there were such agreements for a total of 256 of the 360 residues. Agreements in the amino-terminal third of the molecule were found for 68% of the residues, whereas in the remainder of the molecule only 53% of the residues showed such agreements. Only two helix-breaking and two β-breaking tripeptides were inconsistent with the composite predictions by three or more methods. The large number of disagreements among the results for different methods indicates that only very limited information is provided by each method and that the basis on which they operate is not clear. There is no a priori reason for a composite prediction to be more reliable than any individual prediction, and such a procedure does not permit the determination of an unambiguous secondary structure. Since these methods were applied to lac repressor before any three-dimensional crystallographic structure was known, the methods may ultimately be evaluated should such a structure become available.
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    Biopolymers 18 (1979), S. 2645-2657 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformational changes and binding behavior of tetranactin on complexation with sodium, potassium, rubidium, cesium, and ammonium ions were investigated by the measurements of proton magnetic resonance, ir, and Raman spectra. It has been clearly shown that alkali cations coordinate to the oxygen atoms of both the carbonyl group and the tetra-hydrofuran ring, but the ammonium ion coordinates only to the oxygen atom of the tetrahydrofuran. Among the alkali cations the potassium ion most strongly coordinates to the tetrahydrofuran oxygen atoms. The complexation with larger cations induces an expansion of the cavity of the macrocyclic ring of tetranactin and smaller cations contract the cavity. The evidence is revealed by the coupling constants of the methylene protons and the frequency separation between the carbonyl stretching vibrations of the ir- and Raman-active modes. The conformations of the cation complexes in the solid are maintained in solution but that of the cation free form is not.
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  • 82
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Intercalation-site geometries are generated for a tetramer duplex extracted from B-DNA. Glycosidic angles and puckers of the deoxyribose sugar groups bonded to base pairs BP1 and BP4, namely, those at either end of the tetramer duplex, are assumed to be those of B-DNA to insure continuity. All possible geometrical conformations for combinations of C(2′)-endo, C(3′)-endo, C(2′)-exo, and C(3′)-exo sugar puckers are determined for the tetranucleotide backbone. Those with minimum energy are selected as candidates for intercalation sites. Calculations reveal two pairs of physically meaningful families of intercalation sites which occur in two distinct regions, I and II, of helical angles which orient BP2 relative to BP3 and with the helical axis disjointed between these base pairs. For each site I and II within BP2 and BP3, there are two distinct backbone conformations, A and B, connecting BP3 to BP4 or BP1 to BP2 which do not disrupt backbone conformations connecting BP2 to BP3. Hence two pairs, IA and IB, and IIA and IIB, of intercalation sites exist in which the sugar puckers along the backbone of the tetramer alternate from C(2′)-endo to C(3′)-endo on the backbone (5′p3′) connecting BP2 to BP3. The glycosidic angles of the C(3′)-endo sugar χ3γ are, coincidentally, 80° ± 2° for both conformations γ = A and B connecting BP3 to BP4 along the phosphate backbone (5′p3′). Consistent with the theoretical results, the experimental unwinding angles can be grouped into two categories with absolute values of 18° and 26°. The theoretical unwinding angles for sites IA and IB of 16° and for sites IIA and IIB of 20° occur for a displacement of -0.8 Å in the helical axes of BP2 and BP3 and for a 100% G·C composition, with a decrease depending on the amount of A·T base pairs present. Ratios of theoretical unwinding angles of sites I and II, which range from 0.75 to 0.84 for the two principal sites, compare well with the experimental value of 0.71. The theoretical results, in agreement with experimental observation, provide a new interpretation of the nature and conformation of the possible binding sites. Conformations obtained from these studies of intercalation sites in a tetramer duplex are used to rationalize the well-known neighbor-exclusion principle. The possibility of violation of this principle is demonstrated by the existence of two families of physically meaningful conformations. Conformations of unconstrained dimer duplexes are also obtained, one of which corresponds to the experimental crystal structure of ethidium-dinucleoside complexes, but these cannot be joined to the B-DNA structure. Backbone conformations of the tetramer duplex can be constructed until the base-pair separation reaches 8.25 Å, which may limit the molecules that can intercalate.
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  • 83
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: As a continuation of previous papers [Biopolymers (1976) 15, 879; (1978) 17, 1508], the low-frequency dielectric relaxation of DNA solutions was studied with a four-electrode cell and the simultaneous two-frequency measurement. Below a critical concentration, the dielectric relaxation time agrees with the rotational relaxation time estimated from the reduced viscosity and is almost independent of DNA concentration Cp, and the dielectric increment is proportional to Cp. The critical concentration is approximately 0.02% of DNA for molecular weight Mr 2 × 106 and 0.2% for Mr 4.5 × 105 in 1 mM NaCl. Dielectric relaxations are compared for samples before and after deproteinization, and the protein contamination is found to have a minor effect on the dipole moment of DNA. The effect of a mixed solvent of water and ethanol on the dielectric relaxation of DNA is well interpreted in terms of changes in viscosity and the dielectric constant of the solvent, assuming that the relaxation arises from rotation of the molecule with a quasi-permanent dipole due to counterion fluctuation.
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  • 84
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    Biopolymers 18 (1979) 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 85
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    Biopolymers 18 (1979), S. 2911-2911 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 86
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Conformational energies of α- and β-D-glucopyranoses were computed by varying all the ring bond angles and torsional angles using semiempirical potential functions. Solvent accessibility calculations were also performed to obtain a measure of solvent interaction.The results indicate that the 4C1 (D) chair is the most favored conformation, both by potential energy and solvent accessibility criteria. The 4C1 (D) chair conformation is also found to be somewhat flexible, being able to accommodate variations up to 10° in the ring torsional angles without appreciable change in energy. Observed solid-state conformations of these sugars and their derivatives lie in the minimum-energy region, suggesting that the substituents and crystal field forces play a minor role in influencing the pyranose ring conformation. Theory also predicts the variations in the ring torsional angles, i.e., CCCC 〈 CCCO 〈 CCOC, in agreement with the experimental results. The boat and twist-boat conformations are found to be at least 5 kcal mol-1 higher in energy compared to the 4C1 (D) chair, suggesting that these forms are unlikely to be present in a polysaccharide chain. The 1C4 (D) chair has energy intermediate between that of the 4C1 (D) chair and that of the twist-boat conformation. The calculated energy barrier between 4C1 (D) and 1C4 (D) conformations is high - about 11 kcal mol-1.
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  • 87
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    Biopolymers 18 (1979), S. 3043-3065 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Earlier determinations of density gradient proportionality constants β0, density distributions ρ(r), and the effect of pressure on density gradients in the analytical ultracentrifuge have been of limited precision and usefulness in the study of proteins and polypeptides. Reasons for these difficulties are that numerous intermediate relationships were required in the calculations, and the density ranges studied were generally above 1.2 g/ml. Relations are derived in the present paper to directly compute β0(ρ) values and β0′(ρ) values from the original data without any intermediate expansions or approximations. Data are presented for CsCl, CsBr, and Cs2SO4 and compared with literature values. Density distributions are computed for all three salts under a wide variety of experimental conditions of density, column length, and angular velocity. These values of ρ(r) and re are obtained by a numerical iterative technique. Values obtained by this new method are compared with values obtained using closed-form expressions. The effects of pressure on the composition density gradient for the three salts given above are calculated and found to be significant for Cs2SO4 solutions.
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  • 88
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    Biopolymers 18 (1979), S. 3077-3087 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We present evidence for structures of two ordered forms of polyxanthylic acid based on ir spectroscopy, pH titrations, and thermal transitions. Over the pH range ∼6-9.5, the structure is a four-stranded helix with alkali metal ions specifically complexed in the central channel. These internal counterions stabilize the structure by complexing with carbonyl oxygens and by partial screening of electrostatic repulsion caused by ionization of the xanthine residues in this pH range. Below pH 5, the structure is quite different and much more stable. Our data are consistent with a six-stranded helix in which both carbonyl oxygens and both NH protons are hydrogen bonded.
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  • 89
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An ir-absorption and Raman-scattering study, in the solid state, has been carried out on monodispersed, N- and C-protected homooligopeptides (number of residues, n, from 2 to 7) of L-valine, L-isoleucine, and L-phenylalanine. The amide I, II, III, V, and vNH regions have been examined. Some deuterated (ND) samples have been examined to complete the assignments. L-Phenylalanine dipeptide displays spectral characteristics compatible with the parallel β-structure; L-isoleucine and L-valine dipeptides are probably in a distorted structure. A mixture of parallel and antiparallel extended chains cannot be excluded for the peptides with n = 3. In the amide I region the spectra of peptides with n ≥ 4 show the existence of the β-conformation. The problem of chain orientation within the pleated-sheet structure is discussed on the basis of a recent theoretical treatment of vibrational interactions of the amide I mode.
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  • 90
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The stepwise synthesis and conformational studies of the N-terminal helical partial sequence of the membrane-modifying polypeptide antibiotic alamethicin are described. The polyoxyethylen esters of the fragments N-t-Boc-L-Pro-Aib-Ala-Gln-Aib-Val-Aib-Gly-OH and N-Ac-Aib-L-Pro-Aib-Ala-Aib-Ala-Gln-Aib-Val-Aib-Gly-OH are synthesized using polyoxyethylene (molecular mass 10,000) as solubilizing support. CD spectra of each intermediate in ethanol show α-helix formation of the N-protected peptide polymers beginning with the nonapeptide and of the N-protonated sequences beginning with the decapeptide. Compared to the helix of alamethicin, temperature- and solvent-dependent CD measurements indicate analogous conformational behavior. The results suggest that in lipophilic media the alamethicin helix can extend the full length of the partial sequence between the two proline residues and that aqueous media favor an increase of random-coil conformation.For model studies of the particular lipid interaction of alamethicin, the stepwise synthesis of peptides with the alternating (Aib-L-Ala)n sequence (n = 1-7) was carried out on a polyoxyethylene support (molecular mass 6000). CD and ORD studies in ethanol showed a change from the random coil to a right-handed α-helix with increasing peptide length. This change is observed for the N-protected peptides at a chain length of 8 residues and for the N-protonated peptides at a length of 9 residues. The comparison of the CD data of free and polyoxyethylene-bound peptides revealed that the solubilizing polymeric support cannot induce conformational changes. The intensities of the CD bands of t-Boc-(Aib-L-Ala)n-OPOE (n ≥ 6) are higher than those of alamethicin, and these model peptides show similar temperature and solvent inducible changes of their helix contents.
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  • 91
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    Biopolymers 18 (1979) 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 92
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    Biopolymers 18 (1979), S. 539-552 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The three-dimensional structure of putrescine diphosphate has been solved by x-ray diffraction analysis. The structure reveals the detailed interaction between the amino groups of putrescine and the phosphate residues in which hydrogen bonding and electrostatic forces play a predominant role. The structure serves as a useful model for understanding the interaction of amines with nucleic acids both in a sequence-specific and non-sequence-specific fashion. In particular, a model is proposed for the interaction of the E-amino group of lysine with regions of DNA containing adenine-thymine sequences.
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  • 93
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    Biopolymers 18 (1979), S. 609-623 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A general model for the large-scale, time-independent structure of duplex DNA is developed based on elastic considerations. The general conditions of elastic equilibrium are given. These equations are solved for the equilibrium shape of stressed duplex DNA, based on the assumption that the double helix behaves mechanically as a symmetric, linearly elastic rod. It is shown that, in general, two orders of superhelicity will arise at equilibrium. Several possible applications of this approach to the supercoiling of closed circular DNA are described.
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  • 94
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    Biopolymers 18 (1979), S. 663-680 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Temperature-dependent conformational transitions of deoxyoligonucleotides have been monitored by measuring 31P chemical shifts, spin-lattice relaxation times (T1), and 31P-{H} nuclear Overhauser enhancements (NOEs). The measured NOE ranged from 30 to 80%, compared to the theoretical maximum of 124% for a dipolar relaxation mediated by rapid isotropic rotation. The observed 3′-5′ phosphate diester 31P T1 showed a similar temperature dependence over the range 2-75°C for both double- and single-stranded oligonucleotides, and for dinucleotides. The results show that dipole-dipole interactions dominate the internucleotide phosphate relaxation rate in oligonucleotides. The same is true of terminal phosphate groups at low temperature; but at higher temperature another process, possibly due to contamination by paramagnetic ions, becomes dominant. The rotational correlation time τR calculated from the dipole-dipole relaxation rate of the internucleotide phosphate in d(pA)2 at 16°C is τR = 5.0 × 10-10 sec, implying a Stokes radius for isotropic rotation of 7.6 Å. The T1 and NOE values for the double-helical octanucleotide d(pA)3pGpC(pT)3 are consistent with dominance of dipole-dipole relaxation and isotropic rotation of a sphere of radius 14 Å, a reasonable dimension for the double helix. Activation energies for the rotation of dinucleotides range from 4 to 6 kcal/mol, close to the value of 4 kcal/mol expected for isotropic rotation. In order to test the possible effect of internal motion of correlation time τG on the results, we considered a model in which the nucleotide chain rotates about the P-O bonds. Comparison of the calculation with our experimental results shows that internal motion with τG ≅ 10-9 sec, as found from other studies to be present for large nucleic acids, would not influence out T1 and NOE values enough to be distinguished from isotropic rotation. However, we can conclude that τG cannot be as fast as 10-10 sec, even for dinucleotides.
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  • 95
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    Biopolymers 18 (1979), S. 739-742 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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  • 96
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Measurements have been made of the high-resolution nmr spectra of the polyamino acids poly[N5-(2-hydroxyethyl)-L-glutamine] and poly[N5-(4-hydroxybutyl)-L-glutamine] in mixed deuterium oxide and water solvent at varying pressures from 1.03 to 3163.7 kg/cm2. The results are compared with previously reported results for the polymer poly[N5-(3-hydroxypropyl)-L-glutamine] under similar conditions. The significance of the behaviour of the polymers is considered in terms of the effect of the presence of hydrophobic residues in their side chains.
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  • 97
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    Biopolymers 18 (1979), S. 765-788 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Exact solutions are obtained for the time dependence of the extent of irreversible binding of ligands that cover more than one lattice site to a homogeneous one-dimensional lattice. The binding may be cooperative or noncooperative and the lattice either finite or infinite. Although the form of the solution is most convenient when the ligand concentration is buffered, exact numerical or approximate analytical solutions, including upper and lower bounds, can be derived for the case of variable ligand concentration as well. The physical reason behind the relative simplicity of the kinetics of irreversible as opposed to reversible binding in such systems is discussed.
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  • 98
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    Biopolymers 18 (1979), S. 887-898 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The preparation of lamellar single crystals of mannan[poly((1 → 4)-β-D-mannose)] is described. Electron diffractograms clearly identify the perpendicular orientation of the chain axis with respect to the lamellar surface. Since the degree of polymerization is 40 or less, no conclusion is made as to chain folding. The unit cell corresponds to the mannan I structure derived from x-ray fiber data on oriented algal mannan. The baseplane dimensions found were a = 7.22 Å and b = 8.92 Å, and the systematic absences observed confirm the proposed P212121 group. It was found that cellulose microfibrils from Valonia ventricosa and bacterial cellulose could serve as extended chain nuclei for inducing oriented crystallization of mannan on cellulose. This produces a shish-kebab type of morphology.
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  • 99
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    Biopolymers 18 (1979), S. 939-957 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A general approach to the problem of molecular conformation is advanced. We describe a formalism that permits experimental and theoretical information to be incorporated into a set of upper and lower bounds on intramolecular distances. Structures (conformations) meeting these bounds can be readily generated and compared with each other. To illustrate the use of the method, we have employed a simple “firehose” model for protein folding to predict the long-range hydrophobic interactions in a small protein: pancreatic trypsin inhibitor. Models of this type lead to the proper hairpin turns and a reasonable set of long-range contacts for this protein. Application of the distance geometry method then yields backbone conformations with errors of 4-8 Å compared to the native structure. We discuss both the merits and shortcomings of the firehose model and the relation between distance geometry and energy minimization techniques.
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  • 100
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    Biopolymers 18 (1979), S. 1009-1019 
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: CD spectra of calf thymus, C. perfringens, E. coli, and M. luteus DNA have been measured in the vacuum-uv region to about 168 nm for the A-, B-, and C-forms. The positive band at about 187 nm and the negative band at about 170 nm found for each type and form of DNA are sensitive to the source of the DNA and the base-base interactions of the double-stranded helix. The A-form spectra confirm that these bands are indeed sensitive to secondary structure. In the near-uv, the CD of B-form DNA is well analyzed as a linear combination of 27% A-form and 78% C-form. However, an analysis of the extended spectrum demonstrates that the near-uv analysis is not correct. The extended analysis shows that the base-base interactions are similar for B- and C-forms in solution, which implies that these two forms have nearly the same number of base pairs per turn. Various types of CD difference spectra are also discussed.
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