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  • 05. General::05.09. Miscellaneous::05.09.99. General or miscellaneous
  • Archäobotanik
  • Chromatography
  • 1975-1979  (8)
  • 1
    Electronic Resource
    Electronic Resource
    Amino acid utilization patterns in clostridial taxonomy (1979)
    Springer
    Archives of microbiology 123 (1979), S. 137-141 
    Details
    ISSN: 1432-072X
    Keywords: 2-aminobutyric acid ; 5-aminovaleric acid ; Glutamic acid ; Lysine ; Proline ; Tyrosine ; Polyamide layer ; Chromatography ; Clostridia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The polyamide layer technique for the chromatographic separation of dimethylaminonaphthalene sulphonyl amino acids has been adapted to the qualitative analysis of amino acids in media before and after the growth of micro-organisms. The method has been used to study the amino acids metabolized by cultures of proteolytic clostridia growing in a medium consisting of an acid hydrolysate of casein as a source of amino acids and small amounts of yeast extract and trypticase as sources of growth factors. The chromatograms of the media after growth showed which amino acids were used and which new amino acids were produced. Clostridium botulinum type F (proteolytic), C. ghoni, C. mangenoti and C. putrificum were found to reduce proline to 5-aminovaleric acid and to produce 2-aminobutyric acid, properties they shared with C. sporogenes and C. sticklandii. C. botulinum type G and C. subterminale used glycine, lysine, serine, and arginine but in contrast to C. sticklandii they neither reduced proline to 5-aminovaleric acid nor produced 2-aminobutyric acid. Both organisms oxidized phenylalanine, tyrosine and tryptophan to phenylacetic acid, p-hydroxyphenyl acetic acid and indole acetic acid respectively. C. lituseburense and C. scatologenes used serine, threonine and arginine and produced 2-amino butyric acid and ornithine. C. lentoputrescens, C. limosum and C. malenomenatum resembled C. tetanomorphum by using glutamic acid and tyrosine. The chromatograms always showed the physiological group to which an organism belonged and in some cases were characteristic of the species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446812
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  • 2
    Electronic Resource
    Electronic Resource
    Simultaneous analysis of two or more samples on the same chromatographic column (1979)
    Springer
    Chromatographia 12 (1979), S. 160-164 
    Details
    ISSN: 1612-1112
    Keywords: Chromatography ; Computer application ; Signal processing ; Simulation ; Correlation techniques
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Pseudo random binary and ternary sequences (PRBS and PRTS) have already been used to identify impulse responses of linear systems—in particular, we have shown [1,2], how decorrelation techniques allow the chromatogram of a given sample to be obtained by injecting the product following a PRBS or PRTS law. In this paper an additional difficulty is overcome by injecting in parallel two different samples onto one column, then obtaining simultaneously but separately the two chromatograms by use of numerical decorrelation. We present the theoretical justification and the result of numerical simulation in the case of a pseudo random ternary sequence injection law. A successful experiment on an actual chromatograph using a pseudo random binary sequence injection law is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02314871
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  • 3
    Electronic Resource
    Electronic Resource
    How to approach preparative liquid chromatography (1979)
    Springer
    Chromatographia 12 (1979), S. 139-146 
    Details
    ISSN: 1612-1112
    Keywords: Preparative ; Liquid ; Chromatography ; Volume ; Overloading
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This work shows the influence of the injection volume on peak broadening owing to the increase of the sample concentration; use of the maximum injection volume is advocated. Once the chromatographic system is defined, a method for calculating the dimensions of the preparative column is developed. Applications of this method are shown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02314866
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  • 4
    Electronic Resource
    Electronic Resource
    Separation of radiochemically-pure chlorophyllsa andb (1979)
    Springer
    Chromatographia 12 (1979), S. 808-809 
    Details
    ISSN: 1612-1112
    Keywords: Chlorophyll ; Chromatography ; Radioactive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The purity of radiochlorophyllsa andb when separated by HPLC is 99.5% at a 95% confidence counting level. The minor degradation compounds of chlorophylla which follow chlorophyllb do not overlap with it and interfere with the specific activity of the latter as they do in TLC or paper chromatography
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02260663
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  • 5
    Electronic Resource
    Electronic Resource
    Comparison between different resolution equations (1979)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 193-194 
    Details
    ISSN: 0935-6304
    Keywords: Chromatography ; Theory ; Resolution equations ; Comparison and evaluation; equation (4) preferred ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240020410
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  • 6
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    Heterogeneity of vertebrate luteinizing hormone-releasing hormone (1979)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1979-10-05
    Description: Radioimmunoassay and chromatography analyses of hypothalamic luteinizing hormone-releasing hormone (LHRH) have demonstrated the presence of LHRH-like immunoreactive peptides in a wide range of vertebrates. Contrary to previous reports, the molecule differs in various vertebrates. Avian, reptilian, and teleostean LHRH's are chemically distinct from the mammalian peptide but are in themselves indistinguishable. However, amphibian LHRH appears to be identical to the mammalian peptide. These findings have interesting evolutionary implications.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉King, J A -- Millar, R P -- New York, N.Y. -- Science. 1979 Oct 5;206(4414):67-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/384514" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; *Biological Evolution ; Chromatography ; Gonadotropin-Releasing Hormone/*analysis/immunology ; Hypothalamus/analysis ; Radioimmunoassay ; Species Specificity ; Structure-Activity Relationship
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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    PAPER CURRENT
  • 7
    Electronic Resource
    Electronic Resource
    Interpretation of the real plate number concept: A fundamental analysis (1978)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 1 (1978), S. 41-46 
    Details
    ISSN: 0935-6304
    Keywords: Chromatography ; Theory ; Chromatographic systems analysis ; Real plate number ; Fundamental interpretation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The real plate number concept is interpreted in terms of the fundamental properties of a chromatograph. It is shown that the linear relationship between the total peak width at half height, (b0.5)T, t, and k is only an approximation of the more general linear relationship between (b0.5)2T, t and (1 + k)2. In the case where the relationship between (b0.5)T, t and k approaches linearity, it is concluded, firstly, that b0 = a, i. e., b0 is a column property and independent of extra-column phenomena and, secondly, that nreal=ntheor. When the relationship becomes non-linear, b0 is determined partly by extra-column phenomena, but not in terms of a useful relationship. It is concluded that it is unnecessary to introduce the parameters a, b0 and nreal, since they all have counterparts in the established theory of chromatography. These counterparts prove to be equally useful in the practical analysis of chromatographic systems.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240010109
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  • 8
    Electronic Resource
    Electronic Resource
    Chromatography with Supercritical Fluids (1978)
    Weinheim : Wiley-Blackwell
    Angewandte Chemie International Edition in English 17 (1978), S. 738-746 
    Details
    ISSN: 0570-0833
    Keywords: Chromatography ; Supercritical fluids ; Analytical methods ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Chromatography with supercritical fluids unites the features of both gas chromatography and liquid chromatography, yet retains special characteristics of its own. The diffusion coefficient and particularly the viscosity of fluid phases may approach values for low-pressure gases, while the solvent power may be similar to that of liquids. However, with supercritical fluids it is possible to control chromatographic separations very effectively by pressure programming, since the solubility increases with increasing density. Temperature programming, on the other hand, can have the opposite effect to that in gas- or liquid-chromatography since the density decreases with increasing temperature at a given pressure. Supercritical fluid chromatography is primarily of interest for the separation of higher molecular weight compounds. The efficiency of this method of separation is demonstrated on several homologous series. Thus, a styrene oligomer with nominal Mw=2200 can be resolved by a pressure and temperature program into 40 species.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/anie.197807381
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