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  • Artikel  (4)
  • 04. Solid Earth  (2)
  • Verrucomicrobia  (2)
  • Frontiers  (3)
  • Wiley  (1)
  • American Institute of Physics
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  • 1
    Publikationsdatum: 2021-02-15
    Beschreibung: The Favara Grande is a geothermal area located on Pantelleria Island, Italy. The area is characterized high temperatures in the top layer of the soil (60°C), low pH (3–5) and hydrothermal gas emissions mainly composed of carbon dioxide (CO2), methane (CH4), and hydrogen (H2). These geothermal features may provide a suitable niche for the growth of chemolithotrophic thermoacidophiles, including the lanthanide-dependent methanotrophs of the phylum Verrucomicrobia. In this study, we started enrichment cultures inoculated with soil of the Favara Grande at 50 and 60°C with CH4 as energy source and medium containing sufficient lanthanides at pH 3 and 5. From these cultures, a verrucomicrobial methanotroph could be isolated via serial dilution and floating filters techniques. The genome of strain AP8 was sequenced and based on phylogenetic analysis we propose to name this new species Methylacidimicrobium thermophilum AP8. The transcriptome data at μmax (0.051 ± 0.001 h−1, doubling time ~14 h) of the new strain showed a high expression of the pmoCAB2 operon encoding the membrane-bound methane monooxygenase and of the gene xoxF1, encoding the lanthanide-dependent methanol dehydrogenase. A second pmoCAB operon and xoxF2 gene were not expressed. The physiology of strain AP8 was further investigated and revealed an optimal growth in a pH range of 3–5 at 50°C, representing the first thermophilic strain of the genus Methylacidimicrobium. Moreover, strain AP8 had a KS(app) for methane of 8 ± 1 μM. Beside methane, a type 1b [NiFe] hydrogenase enabled hydrogen oxidation at oxygen concentrations up to 1%. Taken together, our results expand the knowledge on the characteristics and adaptations of verrucomicrobial methanotrophs in hydrothermal environments and add a new thermophilic strain to the genus Methylacidimicrobium.
    Beschreibung: Published
    Beschreibung: 637762
    Beschreibung: 6A. Geochimica per l'ambiente e geologia medica
    Beschreibung: JCR Journal
    Schlagwort(e): Verrucomicrobia ; acidophilic ; methanotroph ; hydrogenase ; Methylacidimicrobium thermophilum AP8 ; 05. General
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2021-07-07
    Beschreibung: A number of oil- and gas-producing leases have been operating in Italy in the last decades, many of which are located in the surroundings of tectonically active regions. Identifying human-induced seismicity in areas with high levels of natural seismicity is a difficult task for which virtually any result can be a source of controversy. We implemented a large-scale analysis aiming at tracking significant departures of background seismicity from a stationary behavior around active oil and gas development leases in Italy. We analyzed seismicity rates before and after hydrocarbon peak production in six oil-producing and 43 gas-producing leases, and evaluate the significance of possible seismicity rate changes. In a considerable number of cases seismicity rate results stationary. None of the observed cases of seismicity rate increase after the peak production is statistically significant (at a s.l. = 0.05). Conversely, considering cases of seismicity rate decrease after peak production, our results suggest that the seismicity rate reduction is statistically significant (s.l. = 0.05) around one oil-producing lease (Val d’Agri, Basilicata) and around a cluster of gas-producing leases in Sicily. Our results put in evidence correlated changes between the rates of shallow seismicity and hydrocarbon production in these areas, which are then identified as hotspots requiring more detailed research; assessing actual causal relationships between these processes will require further physically-based modelling. If a physical causative link between these processes exists, then the observed seismicity rate reduction could either be due to increased seismicity during the progressive increase in production before reaching its maximum, or to an actual seismicity rate reduction after that peak. Considering that there is evidence of seismicity occurring before the start of hydrocarbon production, which contrasts with the evident reduction of events observed after the peak production, we think it likely that the seismicity inhibition is a plausible hypothesis. Using a simple model we also calculate Coulomb stress changes in planes optimally oriented for failure, and we show that under some conditions the inhibition of seismicity is feasible in at least one of these cases. We conclude that more efforts to study the mechanisms and the possible consequences of anthropogenically-driven seismicity inhibition are required.
    Beschreibung: This study was performed with the support of Clypea, the Innovation Network for Future Energy financed by the Italian Ministry of Economic Development, Direzione Generale per le Infrastrutture e la Sicurezza dei Sistemi Energetici e Geominerari (MISE—DGISSEG)
    Beschreibung: Published
    Beschreibung: 673124
    Beschreibung: 4T. Sismicità dell'Italia
    Beschreibung: JCR Journal
    Schlagwort(e): regional seismicity ; hydrocarbon production ; correlation analysis ; seismicity rate changes ; Italy, ; anthropogenic hazards ; 04. Solid Earth
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2021-06-14
    Beschreibung: Verrucomicrobial methanotrophs are a group of aerobic bacteria isolated from volcanic environments. They are acidophiles, characterized by the presence of a particulate methane monooxygenase (pMMO) and a XoxF-type methanol dehydrogenase (MDH). Metagenomic analysis of DNA extracted from the soil of Favara Grande, a geothermal area on Pantelleria Island, Italy, revealed the presence of two verrucomicrobial Metagenome Assembled Genomes (MAGs). One of these MAGs did not phylogenetically classify within any existing genus. After extensive analysis of the MAG, we propose the name of "Candidatus Methylacidithermus pantelleriae" PQ17 gen. nov. sp. nov. The MAG consisted of 2,466,655 bp, 71 contigs and 3,127 predicted coding sequences. Completeness was found at 98.6% and contamination at 1.3%. Genes encoding the pMMO and XoxF-MDH were identified. Inorganic carbon fixation might use the Calvin-Benson-Bassham cycle since all genes were identified. The serine and ribulose monophosphate pathways were incomplete. The detoxification of formaldehyde could follow the tetrahydrofolate pathway. Furthermore, "Ca. Methylacidithermus pantelleriae" might be capable of nitric oxide reduction but genes for dissimilatory nitrate reduction and nitrogen fixation were not identified. Unlike other verrucomicrobial methanotrophs, genes encoding for enzymes involved in hydrogen oxidation could not be found. In conclusion, the discovery of this new MAG expands the diversity and metabolism of verrucomicrobial methanotrophs.
    Beschreibung: Published
    Beschreibung: 666929
    Beschreibung: 6A. Geochimica per l'ambiente e geologia medica
    Beschreibung: JCR Journal
    Schlagwort(e): Verrucomicrobia ; acidophilic ; methanotroph ; Ca. Methylacidithermus pantelleriae ; volcanic soil ; 05.09. Miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2021-02-03
    Beschreibung: Gases present in the Earth crust are important in various branches of human activities. Hydrocarbons are a significant energy resource, helium is applied in many high-tech instruments, and studies of crustal gas dynamics provide insight in the geodynamic processes and help monitor seismic and volcanic hazards. Quantitative analysis of methane and CO2 migration is important for climate change studies. Some of them are toxic (H2S, CO2, CO); radon is responsible for the major part of human radiation dose. The development of analytical techniques in gas geochemistry creates opportunities of applying this science in numerous fields. Noble gases, hydrocarbons, CO2, N2, H2, CO, and Hg vapor are measured by advanced methods in various environments and matrices including fluid inclusions. Following the “Geochemical Applications of Noble Gases”(2009), “Frontiers in Gas Geochemistry” (2013), and “Progress in the Application of Gas Geochemistry to Geothermal, Tectonic and Magmatic Studies” (2017) published as special issues of Chemical Geology and “Gas geochemistry: From conventional to unconventional domains” (2018) published as a special issue of Marine and Petroleum Geology, this volume continues the tradition of publishing papers reflecting the diversity in scope and application of gas geochemistry.
    Beschreibung: Published
    Beschreibung: 976190
    Beschreibung: 6A. Geochimica per l'ambiente e geologia medica
    Beschreibung: JCR Journal
    Schlagwort(e): geochemistry ; Atmosphere ; 03. Hydrosphere ; 04. Solid Earth
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
    Standort Signatur Erwartet Verfügbarkeit
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