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  • Polymer and Materials Science  (14,433)
  • Biochemistry
  • 2020-2022  (8)
  • 1965-1969  (8,218)
  • 1960-1964  (6,215)
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  • 1
    Publication Date: 2021-05-19
    Description: The green turtle (Chelonia mydas) has been a species of global concern for decades. As an attempt to study health status of nesting green turtles, Blood biochemistry and hematological values were obtained from 18 clinically normal, nesting female green sea turtles on the northern coast of the sea of Oman. Mean curved carapace length (CCL) was 111±4 cm with a range of 103—122 cm. Barnacles were recorded on three turtles. No fibropapillomas were observed on any of the 18 turtle. The mean PCV was 0.41 (proportion of 1) with a range of 0.30–0.58. No basophils or hemoparasites were detected in any of the 18 turtles tested. All biochemistry and hematological values of green sea turtles were within published reference ranges of healthy sea turtle population. No statistically significant differences were noted between the two anticoagulants for Plasma biochemistry values. Plasma alpha–tocopherol concentrations in the 18 turtles tested was 7.8±2.8 g ml-1 with a range of 0.5-11.8 g ml-1. Plasma retinol was evaluated in 18 turtles and the concentration was 0.4 ±0.1 g ml-1 with a range of 0.2-0.6 g ml-1. The present study provides baseline data of the green sea turtle characterized by biological and non-biological factors, which may provide the basis for future studies.
    Description: Published
    Keywords: Chelonia mydas ; Turtle ; Blood ; Hematology ; Biochemistry
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.891-902
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  • 2
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    In:  http://aquaticcommons.org/id/eprint/22039 | 18721 | 2018-01-25 08:15:09 | 22039 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: This study was carried out to find the effects of 17-ß estradiol (E2) on biochemical parameters, sex steroids and carcass composition in juvenile stellate sturgeon (Acipenser stellatus). One hundred and eighty 5-month stellate sturgeon with an average weight of 11.7 ± 0.2 g were divided into 3 treatments with 3 replicates each with 20 fish per tank. The treatments were included 0, 25 and 50 mg E2/kg diet. At the termination of 7 months feeding trial with different levels of E2, blood biochemical parameters, sex steroids and carcass composition were measured. The results of biochemical parameters including plasma glucose, total protein, cholesterol, triglyceride, calcium and phosphorus showed dose-dependent manner. The lowest and highest levels of all biochemical parameters were observed in fish fed control and 50 mg E2/kg diet, respectively (p〈 0.05). Plasma sex steroids concentrations showed the lowest level of E2 in fish fed control diet, while the highest levels of testosterone and progesterone were observed in this treatment (p〈 0.05). Proximate carcass analysis showed no significant difference in moisture, crude protein, crude fat and ash contents among the treatments (p〉 〉0.05). Final weight, total length, hepatosomatic and viscerosomatic indices were also affected by different levels of E2 (p〈 0.05). The results of present study demonstrated that using E2 in the diet of juvenile stellate sturgeon can influence most of biochemical indices and sex steroids which seems that those were related to the changes of sex ratio and some physiological parameters.
    Keywords: Aquaculture ; Biology ; Chemistry ; Stellate sturgeon ; Sex steroids ; Biochemistry ; Growth ; sex ; composition ; Acipenser stellatus ; juveniles ; fed ; dietary ; levels ; 17-ß estradiol ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 59-74
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/22856 | 18721 | 2018-05-30 19:16:54 | 22856 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreserved spermatozoa, the proportion of C16:0 was significantly increased (p=0.05), while the proportion of C23:0, C22:6 n3, C22:5 n6, and C18:1n9t decreased significantly after freeze-thawing compared with that in fresh semen (p≤0.05). The relative content of saturated fatty acids significantly increased (p〈0.05) but that of polyunsaturated fatty acids decreased (p=0.05) in cryopreserved spermatozoa. Consequently, the ratio of unsaturated/saturated fatty acid was greatly decreased in frozen sperm (p〈0.05). Superoxide dismutase and glutathione peroxidase activities in frozen-thawed sperm increased significantly while activity of catalase did not differ at p〉0.05. Malondialdehyde levels increased after cryopreservation compared to fresh semen (p=0.05). These results indicated that an increase in superoxide dismutase and glutathione peroxidase activities in cryopreserved spermatozoa did not efficiently scavenge reactive oxygen species leading to an increase in lipid peroxidation, a reduction in polyunsaturated fatty acids and finally irreversible loss of sperm motility.
    Keywords: Aquaculture ; Biology ; Chemistry ; Lates calcarifer ; Sperm ; Cryopreservation ; Fatty acid composition ; Antioxidant enzymes ; Biochemistry ; Thailand
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 157-169
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/22282 | 18721 | 2018-03-10 14:54:34 | 22282 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-02
    Description: Hematological and biochemical changes were studied in beluga (Huso huso) weighing 450±50g in the presence of b,1-3, D-glucan as a single intraperitoneal injection (0.3mg/kg body weight) once, and thereafter a chronic exposure to organophosphate, diazinon at concentration of 1.5 mg/L as a continuous bath for 63 days at 22±1ºC. Results of erythrocyte profile in fish treated with glucan, but without diazinon, generally showed significant higher values of RBC (P〈0.05) compare to fish treated with glucan plus diazinon bath, while there was no significant difference (P〉0.05) in the values of HCT, Hb, MCH and MCHC between the two groups. Also, fish treated with glucan, but without diazinon bath, showed significant higher values of RBC than that untreated fish up to 3 weeks post-treatment (P〈0.05), while no significant differences was found in other erythrocytic indices between these two groups (P〉0.05). Furthermore, fish treated with diazinon showed higher levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) than other three groups for 14-28 days post-treatment and then they decreased to the lower level for the rest of experiment. Also, fish treated with glucan-diazinon showed insignificant higher levels of these enzymes up to 21 days post-exposure compare to fish treated with glucan but without diazinon bath (P〉0.05). However, such levels reduced to a lower level in this group for the rest of experiment (P〉0.05). Also, fish treated with glucan-diazinon showed generally lower and higher levels of total protein and glucose concentrations in blood plasma, respectively compared to fish treated with glucan but without diazinon bath (P〈0.05).
    Keywords: Biology ; Fisheries ; Huso huso ; Diazinon ; Glucan ; Hematology ; Biochemistry ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 53-66
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  • 5
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    Training Department, Southeast Asian Fisheries Development Center | Samut Prakan, Thailand
    In:  http://aquaticcommons.org/id/eprint/26326 | 23782 | 2019-03-28 08:33:06 | 26326 | Southeast Asian Fisheries Development Center, Training Department
    Publication Date: 2021-07-24
    Description: Dissolved cadmium, copper, iron, lead and nickel in seawater at different depths were analyzed using the cobalt-APDC coprecipitation technique. The concentrations found were low and within the range found in natural seawater elsewhere. Terrestrial sources, especially near the head of the Gulf of Thailand and the Nakorn Sri Thammarat-Songkhla area on the Thai-Malay Peninsular, were clearly observed especially during the high runoff season. External input and horizontal dispersion dominated over internal recycling and removal in controlling concentration and distribution of iron and copper but it was the opposite for cadmium, nickel and lead where biological removal near surface and bottom regeneration might explain the nutrient type vertical profiles of these elements.
    Keywords: Oceanography ; Biochemistry ; Trace metals ; Vertical profiles ; South China Sea ; Gulf of Thailand ; Malaysia
    Repository Name: AquaDocs
    Type: book_section
    Format: application/pdf
    Format: application/pdf
    Format: 86-99
    Format: 14
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22825 | 18721 | 2018-05-25 17:53:46 | 22825 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: The purpose of this study was to investigate the therapeutic effects of propolis on biochemical and microbiological parameters in muscle tissues of carp (Cyprinus carpio, Linnaeus 1758) exposed to arsenic. A sublethal concentration of arsenic (0.01 ppm) and/or 0.01 ppm propolis were administered to fish for seven days. Biochemical parameters [pH, lactic acid, total volatile basic nitrogen (TVB-N), and malondialdehyde (MDA) values] and microbiological changes (mesophilic and psychrophilic bacteria count) were determined in fillet of carp in control, arsenic only, propolis only and arsenic+propolis treatment groups. Results showed that the levels of MDA, lactic acid and TVB-N increased (p〈0.05) while there were decreases total counts of psychrophilic, mesophilic bacteria and level of pH in arsenic group compared to the control group. Additionally, levels of pH, lactic acid, TVB-N, counts of psychrophilic and mesophilic bacteria in arsenic (0.01 ppm)+propolis (10 ppm) group significantly reduced compared to arsenic group (p〈0.05). In conclusion, propolis can affects some biochemical and microbiologic functions and quality in the fillet of carp exposed to arsenic.
    Keywords: Biology ; Chemistry ; Fisheries ; Arsenic ; Biochemical parameter ; Carp ; Fillet ; Microbiological analysis ; Propolis ; Biochemistry ; Turkey
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 896-907
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  • 7
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    In:  http://aquaticcommons.org/id/eprint/25001 | 18721 | 2018-11-19 09:52:12 | 25001 | Khorramshahr University of Marine Science and Technology
    Publication Date: 2021-07-16
    Description: The oxidation of fat in fish diets due to oxygen penetration, high temperature and metal catalysts is common. The effects of feeding diets containing oxidized fish oil on the growth indices and lipid metabolism in young hybrid sturgeons (Huso huso ♂× Acipenser ruthenus♀) was investigated. Three different diets were implemented to three different groups: a control group, Group OFO_50 (50% oil fish oxidized) and Group OFO_100 (100% oil fish oxidized). 90 young sturgeon hybrids with an average initial weight of 212/6 ± 0/7 g, , were randomly divided into 9 fiberglass tanks with a volume of 700 liters after a 2 week adaptation period. Feeding was performed three times a day (8:00, 14:00 and 20:00) according to the specimens’ appetite during the period of the study. After 6 weeks, sampling was done to assay the growth parameters and biochemical indices. In both oxidized oil-fed groups, adverse impact on growth was seen. Also, a significant reduction in cholesterol and triglycerides was observed in the OFO_100 groups. The results showed that feeding hybrid sturgeons with diets containing oxidized fish oil, cause a negative impact on growth and lipid metabolism. Although a slight increase in the percentage of body weight gain and specific growth rate was recorded, it was undesirable and relevant to an increase in visceral fat.
    Keywords: Aquaculture ; Iran ; Oxidized lipid ; Hybrid sturgeon ; Growth ; Fat metabolism ; Blood ; Biochemistry ; Fish oil ; Diet ; Acipenser ruthenus ; Huso huso
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 78-88
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  • 8
    Publication Date: 2021-05-19
    Description: Based on the data collected during scientific expeditions of RV “Professor Vodyanitsky” in January, April, October 2016 and July 2017, spatial and temporal changes in distribution of microplankton ATP and chlorophyll α as indicators of metabolically active biomass and its photoautotrophic part, respectively, were analyzed. The heterotrophic-photoautotrophic index (HPI) was calculated from ratios of these biochemical parameters and used to assess the stages of production and destruction succession of microplankton community over these seasons. In the investigated area, the sites, which most often demonstrate sharp differences in biochemical parameters of microplankton from the general background, can be considered anomalous and subjected to anthropogenic impact, mainly associated with household and industrial effluents. In general, upon comparison of the distribution of the metabolically active biomass and its production part in the specified seasons the similarity in the values manifested itself in the shallow coastal waters of Crimea: low values for the waters near the western part of the peninsula were assessed to be oligo-mesotrophic; increased values for the waters adjacent to the Kerch Strait in the east were found to be meso-eutrophic. Based on the HPI values, the situation, most promising in terms of development of microplankton biomass and increase in water productivity was observed in the investigated area in autumn. Quite possibly, it is one of the key factors affecting seasonal migration of some fish species from the Azov Sea to the Black Sea in the autumn-winter season.
    Description: На основе данных, полученных в научных экспедиционных рейсах НИС «Профессор Водяницкий» в январе, апреле, октябре 2016 г. и в июле 2017 г., проанализированы пространственно-временные изменения распределения АТФ и хлорофилла α микропланктона как показатели общей метаболически активной биомассы сообщества и фотоавтотрофной его части, соответственно. Из соотношений концентраций этих биохимических показателей рассчитан гетеротрофно-фотоавтотрофный индекс (HPI), который использован для оценки стадий продукционно-деструкционной сукцессии сообщества в указанные сезоны. Участки исследованных акваторий, на которых наиболее часто отражаются резкие отличия биохимических показателей микропланктона от общего фона, можно принять во внимание как аномальные, подверженные антропогенному воздействию, главным образом связанные с бытовыми и промышленными стоками. В целом в порядке сравнения распределения метаболически активной биомассы и продукционной его части в указанные сезоны сходство величин проявилось в прибрежном мелководье Крыма: низкие — с оценкой олиго-мезотрофные — у западной части полуострова, повышенные — с оценкой мезо-эвтрофные — у восточной, в водах, прилегающих к Керченскому проливу. Судя по величинам НРI, в исследованных водах наиболее перспективная ситуация к развитию биомассы микропланктона и повышению продуктивности вод была отмечена в осенний сезон. Вероятно, это является одним из ключевых факторов сезонной миграции некоторых видов рыб из Азовского моря в Черное в осенне-зимний сезон.
    Description: Published
    Keywords: Chlorophyll a ; Microplankton ; Seasonal variations ; Heterotrophic-photoautotrophic index ; Seasonal migrations ; Anthropogenic effects ; Biochemistry ; Meso-eutrophic waters ; Хлорофилл а ; Микропланктон ; Сезонные миграции ; Сезонная изменчивость ; АТФ ; Биохимия
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.80-88
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  • 9
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Poly-L-proline in the molecular weight range 50,000-90,000 has been synthesized from L-proline-N-carboxyanhydride. The molecular weight obtained is dependent on the initiator concentration. The rate constants for the polymerization have been determined by measuring the CO2 evolution. By observation of rotation during the polymerization, it is possible to deduce the conformation of the polymer in the polymerization solution. It is concluded that the immediate product of the polymerization is neither pure form I nor pure form II. The differences between form I and form II of poly-L-proline have been further characterized by measurements of their ultraviolet rotatory dispersion and their ultraviolet spectra.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The base-catalyzed hydrolysis rate of the cationic esters obtained by quaternization of N,N,N′,N′-tetramethylethylenediamine with phenylbromoacetate was studied in buffers and in solutions of partially neutralized polymeric acids. The polyanions were found to be powerful inhibitors of the reaction. This effect was interpreted as due to the association of the ester with the polyanion, from which the catalytically active hydroxyl groups are repelled. It is shown that kinetic data may be interpreted by a procedure similar to the Lineweaver-Burk plot to yield the reactivity of the bound ester and the dissociation constant of the polymer-ester complex.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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