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  • Scanning electron microscopy  (77)
  • Springer  (77)
  • Blackwell Publishing Ltd
  • International Union of Crystallography
  • 2020-2022
  • 2010-2014
  • 1975-1979  (77)
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Schlagwörter
Verlag/Herausgeber
  • Springer  (77)
  • Blackwell Publishing Ltd
  • International Union of Crystallography
Erscheinungszeitraum
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    SEM analysis of amphibian mesodermal migration (1977)
    Springer
    Development genes and evolution 181 (1977), S. 31-40 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Cell migration ; Mesoderm ; Gastrulation ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary At the end of gastrulation, the lateral mesoderm of amphibian embryos migrates ventrally between the ectoderm and the endoderm. The present study is an examination of the morphology of the leading cells of the mesodermal sheet and of the substratum over which they move (the inner surface of the ectoderm). The cells of the leading edge of the mesoderm are generally round, with very short and narrow flattened projections in the forward direction. These projections do not have a “ruffled” morphology, regardless of whether fixation is carried out before or after the ectoderm and mesoderm are dissected away from the endoderm. The inner surface of the ectoderm is covered with fine (450–500A) filamentous extracellular material and the ectoderm cells sometimes extend cytoplasmic processes (approx. 0.1 μ wide) onto the leading surface of the mesoderm or onto adjacent ectoderm cells. These studies indicate that the morphology of cell migration in amphibians is closer to that seen inFundulus than to that characteristic of chick or mammalian cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00857266
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Growth of embryonic chick tibiae in ovo and in vitro: A scanning electron microscope study (1979)
    Springer
    Calcified tissue international 27 (1979), S. 33-40 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Chick embryo ; Bone ; Organ culture ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary The study describes the ultrastructure of the mineralized portion of chick tibiae from 10 days in ovo to 2 days post-hatch. At 10 days a single mineralized cylinder surrounds the diaphysis. On its outer surface columnar trabeculae join to form ridges parallel to the long axis of the bone. These ridges are covered by another cylinder and form the haversian canals. At 11 days vascular invasion of the marrow cavity occurs and resorption of the endosteal surface begins. This type of periosteal deposition and endosteal resorption is repeated during and subsequent to embryonic development. The mineralized portion of 10-day chick tibiae cultured for 2 days in modified BGJ medium was compared with 10-, 11-, and 12-day tibiae in ovo. Cultured tibiae were similar in length and calcium content to 11-day tibiae in ovo. The form of mineral deposited in ovo and in culture was the same, namely, aggregates of spherical mineral clusters. Differences in culture included the following: (a) few concentric cylinders were deposited as compared with tibiae in ovo; (b) trabeculae were not arranged in rows and ridges in culture; (c) osteocytic lacunae were restricted to bases of trabeculae rather than uniformly distributed as in ovo; and (d) the endosteal surface of tibiae in culture appeared etched.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02441158
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Correlation of freeze-fracture and scanning electron microscopy of epiphyseal chondrocytes (1978)
    Springer
    Calcified tissue international 26 (1978), S. 237-241 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Epiphyseal chondrocytes ; Freezefracture ; Scanning electron microscopy ; Cell processes ; Membrane particles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary Chondrocytes in epiphyseal cartilage were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) using freeze-fracture techniques. Freeze-fracture replicas showed large numbers of fingerlike, 0.11–0.15 μm diameter, projections from the chondrocyte surface, with numerous 95–180 Å diameter intramembranous particles associated with both the cell membrane surface and these projections. With SEM, these cytoplasmic projections were also obvious, but appeared collapsed into clusters of globular-shaped projections on the surface of the chondrocytes. With freeze-fracture techniques, in which shrinkage artifacts were essentially eliminated, the cytoplasmic projections were often seen in intimate contact with the extracapsular matrix. However, with chondrocytes prepared by both SEM and conventional TEM, there was evidence of shrinkage, the cytoplasmic projections having little contact with the extracapsular matrix. These findings show that the cytoplasmic processes are not artifacts of tissue processing and provide morphological evidence in support of the hypothesis that matrix vesicles are of cellular origin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02013264
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  • 4
    Digitale Medien
    Digitale Medien
    Scanning electron microscopical study on the fluorosis of enamel in rats (1978)
    Springer
    Calcified tissue international 25 (1978), S. 75-83 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Rat ; Fluorosis ; Enamel ; Scanning electron microscopy ; Low temperature incineration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary Sixteen 58-day-old male rats of Wistar strain, with a mean body weight of 179 g, were divided into two equal groups. Each group of eight animals was maintained for 70 days on drinking water, ad lib., containing no fluorine (control group) and 100 ppm of fluorine (experimental group). All specimens examined were obtained from the incisal portions of the incisors. The following types of enamel specimens were prepared for scanning electron microscopy: (1) acid-etched specimens; (2) acid-etched specimens followed by low temperature microincineration; and (3) fractured specimens. The enamel formed during high fluoride exposure showed marked hypocalcification, that is, the crystallite density in the prism core and interprismatic region was lower than that of control animals. The organic substances appeared to increase in these regions. These changes were prominent in the outer and middle enamel layers. Such changes following fluoride administration appear to indicate an inhibition of enamel maturation, that is, an inhibition of the mineral deposition and/or an inhibition of organic matrix withdrawal.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02010754
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  • 5
    Digitale Medien
    Digitale Medien
    Structure and association of wall fibrils produced by regenerating tobacco protoplasts (1979)
    Springer
    Planta 146 (1979), S. 203-210 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Cellulose ; Microfibrils ; Negative staining ; Nicotiana ; Protoplasts ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A study has been made of the wall fibrils produced by tobacco protoplasts, using scanning electron microscopy in conjunction with negative staining. It has been shown that the fibres seen in scanning electron microscopy correspond to aggregates of microfibrils. These aggregates are only visible where they are lifted clear of the protoplast surface. Negative staining of fixed protoplasts shows that the aggregation of microfibrils into the fibres visible in scanning electron microscopy is probably produced by air-drying. Gentle disruption of microfibrils produces both random broken fragments and bundles of short pieces of fibrillar material about 60 nm in length. This material is present in undisrupted young walls, but not in undisrupted older walls. The microfibrils in young walls seem much more fragile and liable to breakage than those in older walls. These results are discussed in terms of the interpretation of scanning electron microscope images and the mechanism of cellulose microfibril formation by higher plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00388233
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Localization of α-galactomannan on the surface of Schizosaccharomyces pombe cells by scanning electron microscopy (1977)
    Springer
    Archives of microbiology 112 (1977), S. 123-126 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Bandeiraea simplicifolia ; Schizosaccharomyces pombe ; Colloidal gold ; Cytochemistry ; α-Galactomannan-lectin ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Galactomannan was localized by scanning and transmission electron microscopy on the cells and cell walls of Schizosaccharomyces pombe. The markers were prepared from colloidal gold granules labelled with an α-galactopyranosyl-binding lectin isolated from the seeds of Bandeiraea simplicifolia. Part or all of this α-galactomannan was present in the outer layer of the cell wall and was uniformly distributed even on the fission scars.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00429323
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Localization of mannan at the surface of yeast protoplasts by scanning electron microscopy (1976)
    Springer
    Archives of microbiology 109 (1976), S. 9-14 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Candida utilis ; Saccharomyces cerevisiae ; Colloidal gold ; Cytochemistry ; Mannan ; Plasma membranes ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The β(1→3)glucanase of Basidiomycete QM 806 was used to prepare Saccharomyces cerevisiae and Candida utilis protoplasts. Plasma membranes isolated from S. cerevisiae contained a small amount of mannose and traces of glucose and ribose. Randomly distributed α-mannan was detected by scanning electron microscopy at the surface of prefixed protoplasts using colloidal gold labelled with Concanavalin A as a marker. C. utilis protoplasts were also marked with anti-mannan antibodies. Again the distribution of mannan was random. This experiment indicated also that plasma membrane mannan has the same immunochemical determinants as cell wall mannan. It is hypothesized that mannan is mainly located in the outer layer of plasma membranes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425106
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  • 8
    Digitale Medien
    Digitale Medien
    Mating in Chlamydomonas eugametos (1976)
    Springer
    Archives of microbiology 109 (1976), S. 31-35 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Scanning electron microscopy ; Chlamydomonas ; Cell agglutination ; Cell fusion ; Flagella
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A technique has been developed by which mating gametes of Chlamydomonas eugametos can be studied in the Scanning Electron Microscope. A detailed description of the mating process, from the initial flagellar agglutination until the release of free vis-à-vis pairs, is presented. Flagella appear to agglutinate at random points on their surface. This is followed by a rapid increase of the contact area such that they “line-up” tip to tip. Flagella always exhibit a typical position prior to cell fusion. After cell fusion the flagella of a pair separate rapidly while the female have shortened about 33%. In a vis-à-vis pair the plasma bridge has contracted. The observations are interpreted in terms of a specific reorganization of the sexuale aggregate.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00425109
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  • 9
    Digitale Medien
    Digitale Medien
    The surface of the pecten oculi in the pigeon (1975)
    Springer
    Cell & tissue research 157 (1975), S. 457-465 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pecten oculi ; Pigeon ; Surface ; Function ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A scanning electron microscopic study of the pecten reveals the rib-like character of the pectinal folds, the nature of their connections with the base and bridge of the pecten as well as the presence of interconnections between the bundles of superficial collagenous fibres. It has been suggested that the pecten may have a mechanical significance, namely protection of the retina from the excessive movements of the vitreous humour. The relationship between the superficial membrane of the folds which is continuous, the intercellular spaces and the process of diffussion has also been discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00222599
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Scanning electron microscopic studies on the development of the chick retina (1976)
    Springer
    Cell & tissue research 170 (1976), S. 145-159 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neurogenesis ; Retina ; Scanning electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Fixed retinae of chick embryos and chicks of the first week after hatching were fractured and examined with the scanning electron microscope. The matrix cells of the retina proliferate up to the beginning of the second week. The migrating cells are oriented in cell cords. This columnar organization prevails up to the development of the plexiform layers formed as a consequence of the outgrowth of the dendritic and axonal cell processes. Special attention was paid to the differentiation of the ganglion, bipolar and receptor cells, and the radial fibers (Müller cells). Two main morphological patterns are significant for the organization of the retina during neurogenesis: a) the cell to cell contacts of migrating cells and b) the spatial arrangement of Müller cells which could provide guidelines for migration of neuronal elements.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224296
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