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  • Biochemistry and Biotechnology
  • 1980-1984
  • 1960-1964  (46)
  • 1925-1929
  • 1962  (46)
Collection
Keywords
Publisher
Years
  • 1980-1984
  • 1960-1964  (46)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Masthead (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040101
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  • 2
    Electronic Resource
    Electronic Resource
    Editorial (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 1-3 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040102
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  • 3
    Electronic Resource
    Electronic Resource
    Design and operation of a pilot-plant fermentor for the continuous propagation of filamentous microorganisms (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 5-16 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A multi-stage pilot fermentor was designed which consists of nine stainless steel cylindrical compartments 2 ft. in length and 8 in. in diameter, joined end to end, giving a tube with a total length of 18 ft. The whole apparatus was mounted with its axis in a horizontal plane. Each compartment was sealed off from its neighboring compartment by a separatory plate having a 1-in. overflow hole in the upper half. Shafts 9½ feet long extended from each end of the fermentor through the compartments to the center. Agitation was provided by a multitude of stainless steel blades mounted at right angles to the shafts and spaced at regular intervals along the shafts. Stationary, stainless steel comb-shaped baffle plates were installed vertically at the bottom of each compartment to increase mixing efficiency. Constant leaving and re-entering of the fermentation liquor by the blades upon rotation imparts a shattering of the liquid surface thus preventing the accumulation of mycelial aggregates on the walls above the fermentation liquor. The mechanical performance of the described fermentor has proved excellent and several trial fermentations (antibiotic formation and steroid bioconversion) are described.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040103
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  • 4
    Electronic Resource
    Electronic Resource
    Submerged citric acid fermentation of ferrocyanide-treated cane molassesContribution from the Division of Applied Biology, National Research Council, Ottawa 2, Canada. Issued as N.R.C. No. 6722 (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 17-21 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated cane molasses was fermented in 2.5-l. Pyrex tower fermenters using pellets of Aspergillus niger, grown in beet molasses, as an inoculum. The success of the process depended on accurate control of the ferrocyanide concentration in the fermentation mash. Highest yields (up to 80% conversion of available sugar in 8 days) were obtained when the ferrocyanide concentration was 20 p.p.m. at the start of fermentation and then increased to 80-150 p.p.m. at the 24-hr. period. Addition of phosphate, nitrate, or methanol to fermentation mash did not increase rate of acid production or final yield.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040104
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  • 5
    Electronic Resource
    Electronic Resource
    Microbial production of amino acids. I. The synthesis of lysine and threonine by ustilago species (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 23-36 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of a number of Ustilago species, especially Ustilago maydis (DC.) Cda., to produce lysine and threonine was investigated. The organisms were grown in shake flasks or in 10-l. fermentors. Lysine and threonine were found to be excreted into the medium both in the free and bound form. The bound amino acids could be released by acid hydrolysis or by enzymes from autolyzed cells. The optimal conditions for the release by autolysis were, in the case of Ustilago maydis (DC.) Cda., pH 4.3 and 45°C. An enzyme that could liberate lysine from the bound form(s) occurring in the broth was extracted from cells of Ustilago maydis (DC.) Cda. It exhibited an apparent pH optimum near 4.0. The effect of pH and temperature during the growth phase on the yield of lysine and threonine was studied in 10-l. fermentations.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040105
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  • 6
    Electronic Resource
    Electronic Resource
    Microbial production of amino acids. II. The influence of carbon and nitrogen sources and metal ions on growth of Ustilago maydis (DC.) Cda. and on lysine and threonine production (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 37-52 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ability of Ustilago maydis (DC.) Cda. to grow and to produce lysine and threonine was investigated in shake flask cultures. Growth and production of lysine and threonine increased markedly when aeration was increased. The optimal ratio of glucose to diammonium phosphate in the medium seemed to be approximately 10:1. Ammonium phosphate, ammonium sulfate, ammonium chloride, ammonium nitrate, sodium nitrate, urea, aspartic acid, glutamic acid, and glycine were readily used as nitrogen sources. Growth and amino acid production was poor on ammonium carbonate, ammonium acetate, ammonium citrate, trimethylamine, and betaine.U. maydis (DC.) Cda. was found to grow on a number of different carbohydrates. Besides D-glucose the organism could utilize D-mannose, D-galactose, D-fructose, L-arabinose, D-xylose, D-ribose, sucrose, maltose, and the polyalcoholes D-sorbitol, D-mannitol, and i-inositol. Pectin, dextrin, and corn starch treated with α-amylase could also be used but not untreated corn starch or lactose, cellobiose, D-sedoheptulose, glycerol, or D-glucosamine. The formation of lysine and threonine was better with disaccharides and hexoses than with pentoses.No specific effects on the formation of lysine and threonine could be observed from changes in the concentration of calcium, sodium, potassium, magnesium, zinc, iron, copper, manganese, cobalt, or molybdenum. The requirement of metal ions for growth is discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040106
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  • 7
    Electronic Resource
    Electronic Resource
    1-dehydrogenation of steroids by Septomyxa affinis (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 57-63 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: 1-Dehydrogenation of steroids by Septomyxa affinis is brought about by an inducible enzyme. Only soluble substrate steroid is attacked. Individual steroids are dehydrogenated at different relative rates. The pH-activity curve is shallow.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040108
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  • 8
    Electronic Resource
    Electronic Resource
    Kinetics of 1-dehydrogenation of steroids by Septomyxa affinis (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 65-78 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Mathematical models for the kinetics of 1-dehydrogenation of steroids by Septomyxa affinis are considered. A model providing for product inhibition fits experimental data best. Product inhibition was also demonstrated directly.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040109
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  • 9
    Electronic Resource
    Electronic Resource
    Use of ethylene glycol in counter flow electrophoresis and in cold concentration of DNA and protein solutions (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 53-56 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Counter flow electrophoresis was adapted for temperatures down to -5°C. by the use of ethylene glycol. A method is described for evaporation of water solutions containing ethylene glycol at temperatures between -5 and -10°iC. without ice formation. These measures were found not to affect the transforming activity (two markers) of Bacillus subtilis DNA.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040107
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of UV-irradiated nitrogen gas on biological nitrogen fixation in batch cultures of Azotobacter vinelandii (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 87-97 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In batch cultures of Azotobacter vinelandii exposed to air and ultraviolet-irradiated nitrogen, an increases in nitrogen fixation, compared to that of cultures exposed to air and non-irradiated nitrogen, has been noted. This supports the hypothesis that an ionized nitrogen molecule is important for the biological nitrogen fixation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040111
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  • 11
    Electronic Resource
    Electronic Resource
    An apparatus for aeration of tissue cells in suspended culture with controlled gas mixtures (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 79-86 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An apparatus is described for the aeration of a series of tissue cultures at rates which may be varied independently for each vessel. The composition of the aerating gas can be adjusted to any desired percentages of CO2 and O2. While designed particularly for study of the aeration requirements of submerged tissue growth, the equipment is equally adaptable for study of submerged growth of bacterial and other systems. The apparatus has been designed for future scale-up and is adaptable to both the overlay and bubble-through types of aeration. Flow rates necessary for producing mixtures of 5.0% CO2 and 95.0% air or 5.6% CO2, 14.0% O2, and 80.4% nitrogen are given. The described system has been operated satisfactorily and has proved to be an inexpensive and useful research tool.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040110
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  • 12
    Electronic Resource
    Electronic Resource
    Improved shaken flask performance (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 99-103 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Aeration in shaken flasks appears to take place primarily by absorption of oxygen in the thin film of fluid deposited on the flask walls. Marked improvement in aeration efficiency can be had by breaking up these films with an independent baffling surface. This improvement can be combined with an improved closure to give uniform and efficient shaken flask aeration.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040112
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  • 13
    Electronic Resource
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    Masthead (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040201
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  • 14
    Electronic Resource
    Electronic Resource
    Carbon balance in high rate dissimilation of acetic acid in semicontinuous methane fermentation (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 105-125 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The nature of anaerobic methane fermentation has been studied extensively over a period of about three years by means of 10-l. semicontinuous fermenters, with ethyl alcohol and calcium acetate as carbon and energy sources. The inoculum for the two systems was isolated by enrichment techniques from primary sewage sludge and used to initiate the processes. No subsequent seeding was necessary. The fermentation was started with a substrate carbon concentration of 50 mmole/l. which was gradually increased to 400 mmole/l.; this means that an increase of substrate level from 3000 ppm to 24,000 ppm of acetate and from 2300 to 18400 ppm of alcohol medium were effected during the study. Such high acetate tolerance of the methane organism does not appear to have been reported before; it was the results of adaptation by the organisms in a semicontinuous culture for a period of 30 months. Effect of supplementary available nitrogen on the rate of gas production at different carbon levels (acetate) was also studied. It was found from the gas analyses that CH4: CO2 ratios were always more than 2 to 3 times the theoretical values, when acetate was used as substrate carbon. At some stages of acetate breakdown the concentration of hydrogen in the gaseous products was found to be as high as 8-9%, a very significant feature of this fermentation. Although sulphate ions were carefully excluded from the constitutents of the media (Analytical reagent chemicals and double-distilled water used throughout) during the period of study (30 months), contaminants later identified as Desulphovibrio desulphuricans were found throughout the study. Morphological study of the organisms reveal that they are short rods appearing in individual or chain form, highly motile, gram-positive and nonflagellated, similar to Methanobacterium söhngenii Barker.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040113
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  • 15
    Electronic Resource
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    Introductory remarks on symposium on fermentation waste disposal (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 127-128 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040202
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  • 16
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    Application of waste-to-product ratios in the fermentation industries (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 129-138 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040203
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  • 17
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    Laboratory testing of pharmaceutical wastes for biological control (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 139-145 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040204
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  • 18
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    Pharmaceutical waste disposal by soil injection (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 147-151 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040205
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  • 19
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    Land disposal of pharmaceutical manufacturing wastes (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 153-160 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040206
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  • 20
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    Complete biological treatment of antibiotic production wastes (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 161-170 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040207
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  • 21
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    Biological treatment of pharmaceutical wastes (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 171-180 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040208
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  • 22
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    Pharmaceutical waste treatment by the trickling filter, activated sludge, and compost processes (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 197-209 
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    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040210
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  • 23
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    Complete mixing activated sludge treatment of antibiotic wastes (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 181-195 
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    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040209
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  • 24
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    Flotation of Escherichia coli with sodium chloride Presented in part at the 130th National Meeting of the American Chemical Society, New York City, September 1960. This report is based on work supported by the U.S. Army Biological Laboratories, Fort Detrick, Maryland (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 211-222 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040211
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  • 25
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    Flotation of Escherichia coli with some inorganic saltsPresented in part at the 130th National Meeting of the American Chemical Society, New York City, September 1960. This report is based on work supported by the U. S. Army Biological Laboratories, Fort Detrick, Maryland (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 223-230 
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    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040212
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  • 26
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    Masthead (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040301
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  • 27
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    Some effects of turbine size on novobiocin fermentations (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 231-240 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Studies were made of oxygen transfer and power in 20-1. novobiocin fermentations with five sizes (23-60% of the tank diameter) of geometrically similar flatblade turbine impellers. The influence of changes in apparent viscosity on oxygen transfer and power was found to be related to impeller diameter. At constant agitator speed the oxygen availability rate (OAR) decreased with increase in apparent viscosity, but the magnitude of the reduction was less with a small impeller than with a large one. At constant power input the small-diameter impeller (23% of tank diameter) gave an OAR about 8 times that of the large one (60% of tank diameter) when tested in viscous beer, whereas with sulfite or yeast all impeller diameters gave the same OAR. Impeller tip velocity correlated with OAR independently of diameter for the five turbine sizes examined. The power required for optimal novobiocin yields increased with increase in turbine diameter, but the yields were independent of diameter at equal OAR.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040213
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  • 28
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    A modified adsorption method for determining percentage of dead yeast cells (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 247-254 
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    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The adsorption of methylene blue from different aqueous solutions by dead yeast cells follows Freundlich's law when the percentage of dead cells varies from 0 to 100. When the Fink and Kühles methylene blue was used, there was observed a linear relation between the percentage of dead cells and the dye concentration at the equilibrium. The experimental errors were measured. A simpler and more precise colorimetric method for determining the percentage of dead yeast cells is deseribed.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040215
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  • 29
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    Aeration in tower-type fermentors: Effect of tower diameter and number of aeration disksContribution No. 6866, from the Division of Applied Biology, National Research Council, Ottawa, Canada (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 241-245 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The rate of oxygen absorption by aqueous sodium sulphite solutions in tower-type fermentors up to 6 in. in diameter was related both to tower diameter and to the number of sintered Pyrex disks used for dispersing air entering the towers. Maximal rates of absorption were obtained in towers 2.5 in. in diameter. Under comparable conditions, rates of oxygen transfer in towers containing one aeration disk were approximately 30% higher than those in similar towers containing two or more disks. In towers greater than 6 in. in diameter, the absorption rate was independent of vessel diameter and number of aeration disks when more than one disk was used. Bubble coalescence was the main cause of the difference in the rate of oxygen absorption.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040214
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    “Sleeve” stirrer for submerged cultures (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 299-301 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An aseptic agitation system for submerged culture has been devised. It consists of a stirring rod which is isolated from the interior of the culture vessel by a sleeve of flexible plastic tubing.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040305
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    The use of paper electrophoresis in studying the metabolism of pseudomonas species. I. ApparatusThis investigation represents a portion of a dissertation submitted by one of the authors (W. F. D.) in partial fulfillment of the requirements for the Ph.D. degree at the University of Kentucky (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 255-270 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The sandwich paper electrophoresis technique, such as that of Foster (1952b), was modified to permit routine examination of as many as 15 samples of materials such as bacterial cultures, simultaneously, and with greater rapidity than with paper chromatography techniques. By the use of borate buffer at pH 9.6 with aniline hydrogen oxalate for development, materials such as glucose, fructose, 2-ketogluconic acid, and 5-ketogluconic acid could be separated in three hours.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040302
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    Mass cultivation of algae in pure heavy waterBased on work performed under the auspices of the U. S. Atomic Energy Commission (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 281-297 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Strains of the green algae Scenedesmus obliquus, Chlorella vulgaris, and C. pyrenoidosa 7-11-05, and of the blue-green algae Fremyella diplosiphon, Plectonema calothricoides, Phormidium luridum, and Synechococcus lividus have been mass cultured autotrophically in essentially pure D2O. Closed Lucite rocking-box units containing 5 liters of culture media can produce 1 to 2 g. (dry weight) per day of fully deuteriated algae or of fully deuteriated, fully 15N-substituted algae. Racks for six such culture units are readily constructed from commerically available parts. Operation has been routine and essentially trouble-free. The algae produced have proved to be an excellent source of completely isotopically substituted metabolites.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040304
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    Fermentation of long-chain compounds by Torulopsis magnoliae. II. Factors influencing production of hydroxy fatty acid glycosidesPresented in part at the 140th Meeting of the American Chemical Society, Chicago, Illinois, September 3-8, 1961. Issued as N.R.C. No. 6929 (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 271-279 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A strain of Torulopsis magnoliae, an osmophilic yeast isolated from flowers, produced an extracellular hydroxy fatty acid glycoside of sophorose which was easily recoverable as a heavier-than-water “oil”. The yield of this product was greatly increased and the nature of the hydroxy fatty acid portion of the molecule was modified, within limits, by the addition of fatty acid esters, hydrocarbons, or glycerides. Yields of hydroxy fatty acids of up to 90% of the substrate added were achieved. Commercially available substrates such as tall oil fatty acids, molasses, and corn steep liquor could be used. Good aeration was necessary, and control of the concentration of nitrogenous substrates and of temperature improved the yields.
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    URL: http://dx.doi.org/10.1002/bit.260040303
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    Production of eburicoic acid by submerged cultures of Polyporus sulfureus (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 303-309 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed whereby eburicoic acid, a triterpenoid acid, can be produced by growing Polyporus sulfureus in submerged form in either shake flasks or tank fermentors equipped with aeration and agitation systems.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040306
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    Volume increase of kernels of corn and sorghum accompanying absorption of liquid water (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 311-322 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The linearity between weight and volume increases, which takes place when kernels of corn and sorghum are immersed in water, was established experimentally. The rate of volume increase was correlated according to an equation analogous to the diffusion equation. The coefficients of volume increase obeyed the Arrehenius equation. The results would be of practical value in designing steeping vessels, as well as of theoretical interest in elucidating the mechanism of water diffusion in cereal grains.
    Additional Material: 8 Ill.
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    Continuous chemical sterilization of microbiological media with β-propiolactone (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 331-340 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A laboratory bench set-up for the continuous chemical sterilization of microbiological media using β-propiolactone was developed. The apparatus permits the sterilization of up to 4 liters/hr. of liquid media. The parameters of hydrolyzing time and temperature necessary to reduce the β-propiolactone to nontoxic levels were determined using a E. coli-nutrient broth system. The sterilization technique was applied successfully to a variety of media, and its potential usefulness on an industrial scale is discussed.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040309
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    Plastic ampoules for liquid smallpox vaccine (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 323-330 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Manufacturing of plastic (PVC) single-dose ampoules for liquid smallpox vaccine is described. The green stained vaccine is injected in polyvinyl chloride tubes which are then sealed by high frequency welding to make ampoules of 16 mm. length. The tendency for desiccation of the filled vaccine at elevated temperature is considered as an advantage, since it provides a visible indication of previous improper storage. The ampoule is opened by cutting with scissors and vaccine is squeezed out directly on the skin.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040308
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    Influence of the ionic concentration of nebulized suspensions on stability of microbial aerosols (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 341-344 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper shows that the ionic concentration of a cell suspension submitted to nebulization has a marked influence on the stability of the produced aerosol.
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    URL: http://dx.doi.org/10.1002/bit.260040310
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    Masthead (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962) 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260040401
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    Automatic control of dissolved oxygen levels in fermentations (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 345-356 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dissolved oxygen levels in fermentation systems are affected by (a) gas flow rate, (b) degree of agitation, and (c) oxygen partial pressure in the gas. Since the first two variables cannot easily be separated, a method for dissolved oxygen control has been devised based on the variation of oxygen partial pressure in the gas. Oxygen and nitrogen are fed to a special mixing valve capable of varying the proportions of the two gases while keeping the total flow rate constant. A dissolved oxygen electrode and control system adjust the valve setting to automatically maintain a preset oxygen level. Oxygen analyzers, monitoring the inlet and outlet gas streams, provide additional information from which oxygen absorption and uptake rates may be determined. The system provides a means for the quantitative and independent study of aeration and agitation effects. In addition, it offers a basis for routine control of oxygen levels in plant fermentation equipment.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040311
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    Some effects of microbial growths on surfactant properties of fuelsPresented at the “Symposium on Microbial Transformations of Hydrocarbons,” 142nd National Meeting of American Chemical Society, Atlantic City, New Jersey, September 1962. (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 357-367 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Fungi and bacteria taken from fuel systems were allowed to grow in jet fuel (or hydrocarbon)-salt solution mixtures and the layers were analyzed for surfactant properties. The fuels included both JP-4 and JP-5 types and an alkylate bottom fraction. Controlled surface activity was imparted to some samples by adding minute quantities of either petroleum sulfonates or petroleum naphthenates. Analyses after growth periods of 2 to 14 weeks failed to show any significant increases in the surface active properties of any fuel or hydrocarbon sample. In all cases, there was either little change or else the surfactant effect was reduced slightly. Opposed to these findings, examination of the aqueous bottoms revealed that microbial growths did effect increases in their surface active properties.
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    Microbial degradation of asphaltPresented at the 142nd National Meeting of the American Chemical Society, Atlantic City, New Jersey, September, 1962. (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 369-376 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It would appear from these data that indeed asphalt degradation by bacteria does occur and can be rather rapid under laboratory conditions. The types of bacteria capable of degrading asphalt are diverse. The effects of temperature, pH, and oxygen tension on asphalt degradation are important but not always critical. The optimum conditions vary for different organisms. There are differences in the mechanisms of asphalt assimilation and degradation. There is a specificity of action by different bacteria which most likely involves the chemical components of the asphalts and the enzymatic action of the microorganism.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040403
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    Mean light intensity - a useful concept in correlating growth rates of dense cultures of microalgae (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 377-390 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An analysis of the distribution of light within some culture vessels developed at General Dynamics/Electric Boat is presented. The concept of a mean effective light intensity, Ē, is introduced and procedures are developed for computing Ē for any culture vessel geometry and incident light intensity distribution. A correlation is performed indicating the significance of Ēin assessing the growth rate of dense, continuous cultures of microalagae.
    Additional Material: 3 Ill.
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    The effect of antimetabolities on the biosynthesis of tetracyclinesPresented at the meeting of the American Chemical Society, Atlantic City, New Jersey, September, 1962. (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 391-402 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040405
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    Fractionation of cell constituents of Bacillus megaterium in a polymer two-phase system (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 403-410 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The distribution of various cell constituents of Bacillus megaterium has been studied in an aqueous two-phase system containing the two polymers dextran and polythylene glycol. The difference in behaviour between cell walls, lipid granules and soluble cell material are such that a considerable fractionation of a suspension of disrupted cells may be obtained after a few counter-current distributions. A mixture of whole cells and protoplasts can also be fractionated in this phase system. If sodium chloride is added to the phase system, vegetative cells and spores can be separated from each other.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/bit.260040406
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    A new technique for counting colonies developed on membrane filters (1962)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 4 (1962), S. 411-412 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/bit.260040407
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