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  • Cell & Developmental Biology  (70)
  • 1925-1929  (70)
  • 1929  (41)
  • 1925  (29)
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  • 1925-1929  (70)
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  • 1
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In the ovary of the rat the ova arise by proliferations from the germinal epithelium, all cells of which are potential ova. This proliferation begins with the differentiation of the gonad, and may last as long as 369 days postpartum. The embryonic ovary is filled with ova which pass through the typical maturation phases. This continues until five days after parturition. These ova degenerate, none being found in the ovary of the twenty-day rat. After the fifth day postpartum, nuclear development in the ova changes until, by the twentieth day, no typical maturation phases are present. With the degeneration of the embryonic ova the ovary takes on the adult structure.The ovary of the adult female rat shows a modified type of meiosis in the germ cells, while that of the embryo shows the typical phases, indicating that this is the primitive type, with the modified form an acquired characteristic.Ova in a single rat may show both twenty-one and thirty-one chromosomes.Follicle cells are formed from the cells of the germinal epithelium and, like the sex cells, may have both forty-two and sixty-two chromosomes in a single follicle. The lutein cells also show both forty-two and sixty-two chromosomes in a single corpus luteum. The chromosomes of the lutein cells enlarge with the expansion of the cell to a size greatly in excess of the chromosomes of the somatic cells. The theca interna is derived from the tunica albuginea.
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  • 2
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 48 (1929), S. 585-609 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The caeca of fourteen bantam fowls have been studied. These fowls ranged in age from six days' incubation to three years after hatching. Between the fifth and sixth days of incubation rectal caeca arise as evaginations from the intestine at the junction of the ileum with the colon. The develoing caeca closely resemble histologically the intestine to which they are attached.The caeca are essentially devoid of content until about the nineteenth day of incubation, but during the remaining days of incubation are gorged with a bluish-gray material similar to that found in the colon. Thus, an early defecatory function is indicated.In general, the proximal third of the caeca remains histologically similar to the intestine, but the distal two-thirds undergoes regression. The latter involves the atrophy of the epithelium and glands, accompanied by the appearance of lymphoid tissue. Much of the lymphoid tissue eventually disappears, to a large extent by atrophy and dissolution of the leukocytes. However, to some extent, lymphocytes develop into granulocytes which escape with other leukocytes into the lumina of the caeca and there disintegrate.Lymph nodules begin to appear in the caeca about one week after the chick hatches. The leukocytes, at least in part, arise in situ from the reticular stroma. Eosinophils arise in certain areas of the tunica propria, and in the earlier stages of their development resemble large lymphocytes, in the cytoplasm of which basophilic, amphophilic, and acidophilic granules are intermingled.
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  • 3
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    New York, NY : Wiley-Blackwell
    Journal of Morphology 48 (1929) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 4
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    Journal of Morphology 48 (1929), S. 281-316 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The arterial system of Uromastix hardwickii retains many primitive features and shows great resemblance and relationship to that of Sphenodon. The heart is typically reptilian. Two systemics and a pulmonary arch arise from the ventricle. The conus arteriosus is absent. The carotids arise together from the right systemic by means of a small common carotis primaria. A very well-developed ductus caroticus connects each carotid with the systemic arch of its side. The ductus arteriosus (ductus Botalli) is absent.The dorsal aorta is formed by the union of both the systemic arches. The left systemic joins entire while the right one, which is termed systemicocarotid trunk, gives off the carotids, the vertebral, a single subclavian, and two pairs of parietal arteries, before the union. Anterior epigastric arteries are altogether absent. The dorsal aorta gives off fifteen pairs of parietal arteries which are segmentally arranged. All the main branches supplying the alimentary canal and other viscerala organs arise independently of one another, there being no such combination as is found in Varanus.
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  • 5
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A histological study has been made of a series of Phrynosoma testes taken at frequent intervals throughout the year. As it was important to differentiate between the amount of interstitial tissue and the number of cells, it was necessary to take into account the percentage of tissue, total testis volume and the number of cells per unit volume.The results show a definite interstitial-cell cycle. Volume of interstitial tissue and size of individual cells are greatest during the breeding season, but the interstitial-cell number is then minimal. The maximal number of interstitial cells occurs after the close of the breeding season. There is, apparently, no reversion of interstitial cells to a connective-tissue-cell type.The above changes are correlated with the spermatogenetic cycle and with increases in total tubule length, in tubule diameter, and in testis volume. The time in which important changes in ovarian volume occur and at which ovulation takes place is coincident with the testicular changes.Interstitial cells have been stated to have no endocrine function for the reason that their number is at a minimum during the breeding season. If, however, interstitial tissue is responsible for sex characters and activity, the present work would indicate that it is the volume of tissue, and not the number of cells, that is important.
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  • 6
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    Journal of Morphology 48 (1929), S. 385-431 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Direction of peristalsis of the dorsal vessel in prepupa, chrysalis, and adult of Lepidoptera is not constantly forward; phases of forward peristalsis alternate with phases either of backward peristalsis throughout the whole dorsal vessel or of diverging waves originating in the third to fourth abdominal segments, proceeding backward and forward. Periodic reversal in the silkworm begins twenty-four hours after spinning, forty-eight before pupation. Pupation occurs during a vigorous backward phase. Soon after pupation, and sometimes during pupation, backward phases consist of diverging waves. Later, and in the adult, complete reversal intermittently occurs. Backward phases in the pupa are long (e.g., fifteen minutes), the average rate slow and variable, quickening at eight to ten days to that of the adult. Any long backward phase shows a gradual slowing down, except approaching pupation when the pulse quickens to equal that of forward beating. Average rate of forward beating is constant at constant temperature, but absolute rate in a long phase gradually slackens. Pauses (sometimes one and one-half hours long in older pupae) often follow forward phases. Alternating phases in the adult, compared with the pupa, are short, about one minute and usually less than one hundred beats. In old moths the phases become still shorter, often being reduced to single beats. Opposing waves occasionally conflict. Periodic reversal occurs for hours after excision of the dorsal wall with the pericardium and heart. As in ascidians, back pressure cannot explain reversal.
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  • 7
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    Journal of Morphology 48 (1929), S. 543-561 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The parietal fossa or pit is situated in the middorsal line between the ear capsules. From its floor four (sometimes only two) apertures lead into the ear capsules. The anterior apertures are the foramina of the endolymphatic ducts; the posterior ones are the fenestrae. Through the fossa each endolymphatic duct passes from its formen to its external aperture in the dorsal integument, describing in its course a loop with the convexity directed anteriorly. The part of the duct involving the loop is enlarged into an endolymphatic pouch. Into the angle of this loop a small muscle is inserted which is a continuation of the anterior trunk muscles, or one taking a more lateral origin from the edge of the fossa. The fenestra may or may not be closed by a definite fenestral membrane. The posterior semicircular canal (posterior utriculus) bears a peculiar relation to the fenestra. The endolymphatic pouch, like the sacculus, contains otoconia, and sometimes siliceous sand grains. Various functions have been assigned to the endolymphatic organ. There is no convincing experimental evidence as to its significance.
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  • 8
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    Journal of Morphology 47 (1929) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 9
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    Journal of Morphology 47 (1929), S. 335-413 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An attempt is made to define lips, and on Danforth's interpretation of homology, homologous lips are found at certain stages of development in some representatives of all classes of vertebrates. The primary lips characteristic of selachians, after the maxillary and premaxillary bones have developed within the territory of the upper lip (toadfish, cod), may disappear (trout, Spelerpes), accompanied by a forward migration of the lower jaw. The secondary lips of higher forms are first indicated in certain teleosts and amphibians. Lips vary in structure to accord with their physiological functions, whether sensory, prehensile, or adhesive. Lips of the cod are highly sensory; those of the tadpole and of grazing animals, in different ways, are notably prehensile; the lips of petromyzon and the vampire, having abundant villi, are most effectively adhesive. Therefore, the smaller villi of the lips of suckling animals are presumably for tight adhesion to the nipple. The opossum and rat, however, nurse before their lips have developed. No free macroscopic villi are found on human lips, but there is a zone of thick epithelium tending to form villi. Such a zone is shown to be a widespread feature of vertebrate lips.
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  • 10
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    Journal of Morphology 47 (1929), S. 415-433 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The diploid number of twenty-six chromosomes was found in the mature parthenogenetic female-producing eggs and also in the somatic cells of the female embryos developing from such eggs. In the maturation stages of a few of these eggs the chromosomes were markedly larger than in the corresponding stages of the majority of the eggs. Whether this size difference of the chromosomes is correlated with male- and female-producing individuals has not been determined. The mature parthenogenetic male-producing eggs contain the haploid number of thirteen chromosomes, and this number was found also in the somatic cells of the young male embryos. The mature sexual eggs contain thirteen chromosomes.In spermatogenesis the secondary spermatocyte divisions are usually omitted and the secondary spermatocytes develop directly into the motile spermatozoa containing thirteen chromosomes. A few, however, of the secondary spermatocytes divide, forming spermatids containing fewer than thirteen chromosomes. These cells develop into the non-motile and rudimentary spermatozoa.The motile spermatozoa containing thirteen chromosomes unite with the parthenogenetic male-producing eggs containing thirteen chromosomes, thus producing the fertilized eggs with the diploid number of twenty-six chromosomes. These fertilized eggs develop into female-producing females which reproduce parthenogenetically.
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  • 11
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    Journal of Morphology 47 (1929), S. 519-529 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The liver has a rhythmic function with alternating assimilatory and secretory stages. The height of the assimilatory stage in rabbit's liver is characterized by the following qualities: All the cells are usually expanded and contain much glycogen and few bile components. The bile capillaries are narrow and generally empty. The glycogen content is high (about 13 per cent); the total glycogen weight is also high (about 17 grams). The liver has a relatively heavy weight (about 140 grams), a large volume, notably firm consistency, and a light brown color.The secretory stage at its height has the following characteristics: All cells are shrunken and are rich in bile components, but contain little glycogen. The bile capillaries are expanded and filled with secretion (fixation with barium chloride). The glycogen content is low (about 1 per cent), and the total glycogen weight is also low (about 1/2 gram). Weight of liver low (about 50 grams). Volume small and consistency fairly flaccid. Color dark reddish brown.Between these two extremes are various intermediary stages. Such variations as these probably occur during the normal functioning of the liver in man and also in other animals.
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  • 12
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    Journal of Morphology 48 (1929) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 13
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    Journal of Morphology 47 (1929), S. 555-587 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The germ cells of Lebistes are found in cysts; the younger cysts are toward the cortex. Mitochondria and Golgi apparatus are present. During maturation leptotene, bouquet, pachytene, and diakinesis figures are seen. The spermatocyte chromosomes number twenty-three; an X-Y pair is probably present, though the evidence is not conclusive. In spermatid formation the centriole divides to form a rodlet and an axial filament; the nucleus segregates into two materials, one of which is extruded; the remainder first contracts to a cup, comes in contact with the rodlet, then again forms a sphere. The mitochondria are arranged along the proximal part of the axial filament; the sphere flattens and elongates; the rodlet sinks into the head substance and is enfolded by it. The Golgi remnant is sloughed off with the residual cytoplasm, which disappears at the same time the Sertoli cells show an increase in size, suggesting their ingestion of the spermatid remnants. The mature sperm form spermatozeugmen, which are stored in the testicular canal; they are transferred to the female by aid of the modified anal fin.
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  • 14
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    Journal of Morphology 48 (1929), S. 1-43 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The parenchymal cells in the parathyroids of Rana catesbeiana (Shaw) are found to undergo a very curious cyclic change. The cytoplasm is broken down by a process of cytolysis. The nuclei are decomposed by chromatolysis and pycnosis. The entire parenchyma is finally liquefied, except for a layer of cells lining the capsule.The parathyroid body is reconstructed by young cells which are produced from rather definite growth centers. The process of regeneration begins some time before cytolysis is completed and continues until the entire organ is restored.The cytolytic and regenerative processes transpire in such sequence that the amount of apparently healthy tissue present at any one time is a rather large portion of the total mass normally present.The relationship between the parenchymal tissue and the stroma of the parathyroid is described.
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  • 15
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    Journal of Morphology 48 (1929), S. 563-584 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: From the structure of statocysts, in the so-called phylogenetic tree of the Cephalopoda, the genus Scaeurgus is to be placed on the same branch as Polypus.Meleagroteuthis has the same number of processes as Watasenia, Enoploteuthis, Abralia, Illex, and Stigmatoteuthis, which have ten processes, but in this genus the structure of statocysts seems to be tolerably well developed. For this reason, the author places this genus as a branch between Abralia and Illex.Those three genera - Gonatus, Sthenoteuthis, and Onychoteuthis - which have eleven processes in a statocyst are to be placed in the same branch as Berryteuthis, Gonatopsis, Ommastrephes, Eucleoteuthis, Moroteuthis, and Symplectoteuthis, which have the same number of processes; of these, Gonatus, which has the most primitive structure of the statocyst, is to be put in the lowest position on that branch; but Sthenoteuthis, which has the same structure of the organ as Ommastrephes, is to be placed on the twig near Ommastrephes; and, lastly, Onychoteuthis has the same organ as that of Moroteuthis, which fact induces the author to set this genus in the position nearest to Moroteuthis.The statocyst of Lolliguncula corresponds exactly to that of Loligo, which has twelve processes; this condition puts this genus on the same twig as Loligo.
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  • 16
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    Journal of Morphology 47 (1929), S. 1-36 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The first spermatocytes of Circotettix verruculatus have eleven chromosomes. Five of these are regularly atelomitic and three are telomitic. The other three are variable and may have both diads telomitic, both atelomitic, or one diad telomitic while the other is atelomitic. The locus of fiber attachment and, consequently, the form of the chromosome are constant for the individual. The point of fiber insertion is known to be inherited according to mendelian principles, and is, therefore, a measure of the frequencies of the telomitic and atelomitic diads in a group of individuals.Samples of five geographically different populations were studied with respect to the proportion of atelomitic to telomitic diads in the three variable tetrads. The proportion for each of the three chromosomes was compared with that for the corresponding tetrads from the other localities. The data were subjected to statistical analysis, and significant differences between some of the groups were found in the proportion of atelomitic to telomitic diads in corresponding chromosomes.A possible correlation between these cytological differences among the various localities and the formation of geographical races or subspecies are discussed. An inversion of the portion of a chromosome, which causes an apparent, although not a real, change in the locus of fiber attachment, is suggested as the origin of atelomitic chromosomes. The effect which heteromorphism of synaptic mates may have on crossing-over is considered.
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  • 17
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    Journal of Morphology 47 (1929), S. 37-87 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The vesicular nucleus of this amoeboflagellate is similar in structure in both phases of its life-cycle. It has a fairly large caryosome surrounded by a pericaryosomal area in which there are small oxyphilic pericaryosomal granules on a fine reticulum. On the inner surface of the definite caryotheca is a layer of epithecal chromatic granules.Nuclear division is similar in both amoeba and flagellate phases. During the prophase the nucleus enlarges, and the expanded caryosome becomes resolved into basophilic and oxyphilic components and assumes either an oblong, dumb-bell, or spindle shape. The pericaryosomal granules enlarge, shift about, and eventually become arranged in an equatorial band around the elongated caryosome. In the metaphase the equatorial plate of chromosomes appears after the inward migration of the pericaryosomal granules, accompanied by the formation of a definite intranuclear spindle, usually with polar masses, polar granules, and a centrodesmose. After the poleward migration of the daughter plates of chromosomes in the anaphase, the telophase constriction of the nuclear membrane produces two daughter nuclei with a portion of the spindle remaining outside. The epithecal layer of granules remains in place on the nuclear membrane during the entire process of mitosis. Plasmotomy normally follows mitosis, but may be delayed, giving rise to multinucleate individuals. In the flagellate the blepharoplast usually divides simultaneously with, but independently of, the nucleus. There are many divergences in the details of mitosis, but these are thought to be variations of one type of division rather than examples of different processes.
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  • 18
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    Journal of Morphology 47 (1929), S. 89-133 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During the telophases each chromosome becomes inclosed in an individual sac or vesicle which, together with its contents, is called a chromosomal vesicle. The vesicular membrane is of cytoplasmic origin, but is formed under the influence of the chromosome and a droplet of karyolymph. A precise numerical correspondence between chromosomes and chromosomal vesicles has not been established, but it is evidence that most, if not all, of the chromosomal vesicles retain their individuality during the resting stage and until after the new chromosomes have been fully formed.The transformation of the telophase chromosome into the reticulum of the resting stage and the manner in which a new chromosome is formed from a portion of this reticulum are described in detail. In the early prophases each developing chromosome is embedded in a sheath or matrix of less deeply basophilic material, which disappears before the middle prophase is reached.The formation of chromosomal vesicles is interpreted as a device for doing more rapidly and effectively, under stress of special circumstances, the work that the nucleus must accomplish during the so-called resting stage.
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  • 19
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    Journal of Morphology 47 (1929), S. 135-199 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Definite information concerning the time of development and location of the different imaginal discs of Drosophila melanogaster was needed in order to interpret especially the gynandromorphs, mosaics, and intersexes that have been extensively reported in cultures of this fly. This information was also desirable for many of the mutant types. It was not known, for example, when an organ was reduced or absent, whether its imaginal disc showed a corresponding reduction, or whether it was full size in the larvae and pupae, and failed to carry through to the later stages.Three mutant types with eyes smaller than those of the wild type, namely, lozenge, bar, and eyeless, were examined. It was found that there is a corresponding difference in size as far back as the imaginal disc could be detected. Similarly for the two mutants, vestigial and no-wing. Conversely for the mutant, bithorax, in which the metathorax is larger than the normal and has assumed many of the characters of the normal mesothorax, the imaginal disc was correspondingly enlarged.It follows that the effects of the mutant genes for these characters can be observed in the very earliest condition of the imaginal disc.
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  • 20
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    Journal of Morphology 47 (1929), S. 201-225 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: There are eleven pairs of chromosomes in the somatic cells of the opossum.The sex chromosomes are of the x-x type in the female and the x-y type in the male.The number and type of the chromosomes are constant in the wide variety of tissues and organs studied, except that one dividing giant cell of the spleen showed an 8n number of chromosomes.The arrangement of the chromosomes in equatorial plates is that of an autosomal ring surrounding the centrally located sex chromosomes.
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  • 21
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    Journal of Morphology 47 (1929) 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 22
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    Journal of Morphology 47 (1929), S. 435-478 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The perivisceral fluid of Pheretima indica (Horst) contains five types of leucocytes: lymphocytes, monocytes, granulocytes, lamprocytes, and linocytes. The granulocytes differentiate either from free lymphocytes, from peritoneal epithelial cells lining the leucocytopoietic organs, or from lymphocytes (hemocytoblasts) embedded in these organs. The lymphocyte is a hemocytoblast. The eosinophilic granulocyte is the most numerous of the several types of granulocytes. Morphologically and tinctorially, it resembles the eosinophil of fishes. The eosinophil granule is thought to arise either by a ripening of a basophil granule and to pass through a metachromatic phase during this process, or by being formed immediately without such a ripening process in small hemocytoblasts. The stimulus for the excessive production of eosinophils is thought to be the degree of infection of the leucocytopoietic organs by a species of the gregarine, Monocystis.A series of segmentally arranged leucocytopoietic organs is described for the first time in the oligochaetes. These organs are essentially foldings of the septa and offer sacculations in which leucocytopoiesis may take place.A discussion of the possible phylogeny of the hemocytopoietic organs of the invertebrates and vertebrates is given.
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  • 23
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    Journal of Morphology 48 (1929), S. 45-79 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: It was found that thyroid deficiency in the albino rat (Mus norvegicus albinus) is followed by a trend to increased concentration of epinephrin in the suprarenals. The basis of this lies in the relatively greater retrogression of the cortex as contrasted with the medulla which becomes a relatively greater proportion of the organ as a whole. Confirmatory evidence is found in cell counts per unit area and the part of the disappearance of lipoid from the cortical cells of the suprarenals of thyroidless animals.
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  • 24
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    Journal of Morphology 48 (1929), S. 81-103 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A comparative study of the embryology of the rabbit in races of large and small adult size shows a consistently more rapid rate of cell multiplication and of increase in mass in large-race embryos than in those of small race.This more rapid rate of growth is transm tted by and influenced equally by sperm and egg cell, as is shown by the results of reciprocal crosses.Rate of differentiation is independent of rate of growth and unaffected by it. Consequently, embryos of the large race have attained greater size than those of the small race at corresponding stage of differentiation.The fundamental difference in rate of growth is already in evidence in forty-eight-hour embryos and becomes increasingly clear at later stages. Embryos produced by the large race have undergone about one more cell division at forty-eight hours after mating and so are approximately in the thirty-two-cell stage when embryos of the small race are in the sixteen-cell stage.
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  • 25
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    Journal of Morphology 48 (1929), S. 105-121 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Two types of cytoplasmic inclusions, differing in reactions to vital dyes and to osmic fixation and impregnation have been demonstrated in Peranema trichophorum. The mitochondria are rod-like and lie in more or less spiral rows, forming a single layer beneath the periplast. They are stained supravitally with Janus green, but not with neutral red. They may be demonstrated by staining in iron hematoxylin after Mann-Kopsch fixation. They are also blackened in prolonged osmic impregnation, but are bleached readily with hydrogen peroxide.The small spherical osmiophilic inclusions are scattered in distribution, although sometimes more numerous in the anterior third of the organism. These bodies are stainable supravitally with neutral red, neutral violet, and brilliant cresyl blue. They are densely blackened in osmic impregnation, and are not bleached in the usual treatment with hydrogen peroxide. They are not however, demonstrated by Mann-Kopsch fixation and iron hematoxylin. After being stained supravitally with neutral red, these inclusions may be blackened under direct observation by exposure to osmic vapor in hanging-drop preparations.
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  • 26
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    Journal of Morphology 48 (1929), S. 123-151 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: From a study of over 1000 mothers, the female chromosome number appears to be 2N = 22; N = 11. The male number has not been exactly determined, but is presumably not haploid. Only one maturation division occurs in the parthenogenetic egg, and the authors have seen only one in the sexual egg. During the growth stages of the eggs, the chromatin is totally obscured by a large amount of deeply staining nucleolar material which exhibits several phases. Ultimately, this material is apparently absorbed into the ooplasm. Just before the egg is laid, the ovoid chromosomes, in late prophase or in metaphase, are seen in a germinal vesicle situated always at one side of the egg. The maturation division occurs immediately after egg laying. A degenerate body, hitherto undescribed, is noted in the ripe parthenogenetic egg, situated at the pole opposite the germinal vesicle. It is believed to arise by reorganization of nucleolar substance. The body in the sexual egg desribed by Weismann and Ishikawa ('91) as the Paracopulationzelle is noted and its interpretation by these authors questioned, but, for lack of sufficient evidence, no counter-explanation is offered. The possible relation between experimental sex control and the time of maturation division in the parathenogenetic egg is discussed.
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  • 27
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    Journal of Morphology 48 (1929), S. 153-171 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A description of the gross embryology of the sex apparatus of a common diplopod, the corn millipede. The life-cycle of this animal involves eleven instars extending over a period of three years.In the male the true first legs are shed at the ninth ecdysis and the large clasping appendages are substituted through the last three instars. In the region of the second body somite the fused coxal plate and the penes are developed at the final molt; the second appendages are reduced at this time. The greatest changes occur in the region of the seventh somite, where the legs are shed about the eighth ecdysis and the gonopods developed through the remaining instars. It is evident that these gonopods arise as a modification of the sternites of the seventh somite.In the female the second legs are reduced to vestiges at the final molt. The structures at the mouth of the oviducts are developed from the surrounding tissues and the modified sternites of the reduced second legs. These changes are in progress as early as the seventh instar, but the greatest changes occur at the final ecdysis.
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  • 28
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    Journal of Morphology 48 (1929), S. 173-251 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The purpose of this study was to determine whether structural elements of the cytoplasm can be demonstrated to be specifically concerned with certain physiological processes that are experimentally induced in tissue at the time of fixation. The methods for bringing into view variations in cell structure correlated with cell activity were as follows: the increase of the process of secretion by subcutaneous injection into the living animal of phenol red and of urea; the increase of the process of reabsorption by injection of glucose: the inhibition of reabsorption of sugar by injection of phlorhizin.Variations of the same magnitude in the form, position, quantity, and interrelationships of granules, globules, chondriosomes, and Golgi substance were found to occur in normal untreated animals and in those treated experimentally. Some evidence was found that in cells of the proximal tubule a characteristic form of Golgi body appears which seems to be correlated with increased reabsorption of sugar.
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  • 29
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    Journal of Morphology 48 (1929), S. 253-279 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Technique. This work is based not only on the fixed preparations, but also on fresh cover-slip preparations treated with neutral red or 2 per cent osmic acid for a short time.Golgi elements and fatty yolk. The Golgi elements are hollow vesicular bodies with a distinct osmiophilic rim and a central osmiophobic substance. In the youngest oocyte they form a circumnuclear ring. Gradually the vesicles spread out, grow in size, store up fat in their interior, and give rise to the fatty yolk. On account of their higher refractive index, due to the presence of fat, the Golgi vesicles can be occasionally seen even in the young oocytes without any treatment.Mitochondria. The mitochondrial granules also form a circumnuclear ring and are later distributed uniformly.Albuminous yolk. The albuminous yolk is nucleolar in origin. Early in oogenesis, the nucleous buds off small, homogeneous, and highly chromatic particles in the cytoplasm, which sooner or later disappear. Subsequently, the nucleolus becomes less chromatic and develops vacuolar bodies in its interior, which, becoming vacuolated exactly like the parent nucleolus, migrate into the the cytoplasm. These bodies become more and more chromatic and travel toward the periphery of the egg, where they grow in size. Ultimately they break down into small, homogeneous, and highly chromatic bodies which are the definitive albuminous yolk spheres and which subsequently grow enormously in size.
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  • 30
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    Journal of Morphology 48 (1929), S. 345-369 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The specimen described presents a typical intersexual condition, in which not only the primary sex characters, but also the accessory sex organs and the secondary sex characters are of a mixed type. Both gonads are ovotestes, in which the ovarian as well as the testicular tissues are morphologically normal, but functionally underdeveloped. Stromal hyperplasia is distinct, especially so in the left gonad; it is probably a functional factor in the generative process of the spermatic tissue.Sex transformation in the intersexual frogs may probably be initiated by an abnormality in sex differentiation or in the genetical constitution of sex, and is only subsequently influenced and modified by environmental action. Absolute dominancy of male sex in the process of sex transformation is questionable. There are indications that in frogs sex transformation may possibly occur in a female direction.
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  • 31
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    Journal of Morphology 48 (1929), S. 611-625 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By the use of a new technique it was possible to demonstrate the Golgi granules about the idiosome of spermatids and in the so-called Golgi bead of the mature spermatozoon. Other osmiophile granules in the cytoplasm, assumed to be Golgi granules, aggregate during metamorphosis into groups which formed neutral fat spheres, giving stains with both sudan III and scharlach R. Evidence is adduced supporting the view that the Golgi apparatus is a lipin and probably a phospholipin. The fat spheres produced are probably a result of fatty degeneration in the residual body of the spermatid. They may also serve as the source of lipin in Popa's ‘Lipo-gel’ phenomenon.
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  • 32
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    Journal of Morphology 47 (1929), S. 227-259 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A histological study was made in the guinea-pig of the distribution of melanin pigment in the normal dark-eye and in various mutant types, such as the brown type of dark-eye (b), light and dark salmon-eye (sm), dark red-eye (crB), brown red-eye (crb), albino (ca), pink-eye (p), and pseudopink-eye (P sm b). The genes which affect pigmentation in the eye have definite qualitative or quantitative effects on the production of melanin. Eyes of the genetic constitution ca, p, or sm possess very little pigment. But eyes which have the dominant allelomorphs of these genes, C, P, or Sm, have a large amount of pigment. The non-yellow gene, cr, and the brown gene, b, cause the deeper retinal layer, which is normally intensely pigmented throughout its entirety, to become less pigmented. There is a corresponding reduction in the melanin in the pigmented areas of the iris and choroid. In all types of eyes with reduced pigment, such as the albino, pink-eye, and pseudopink-eye, pigment persists in the deeper retinal layer even when it is almost absent from other regions. Moreover, in all eyes which have less pigment than the normal type there is a decided tendency for chromatophores to be located adjacent to blood vessels. This tendency is outstanding in the iris and quite noticeable in the choroid.
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  • 33
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    Notes: In sections the protoplasm of the spinning gland of Platyphylax designatus Walker appears to be a syncytium with large, branching nuclei, which contain both nucleoli and chromatin granules embedded in a delicate linin reticulum.In the normal gland the nucleus is sharply marked off by a nuclear membrane from the granular, homogeneous cytoplasm. The Golgi apparatus appears as rings, loops, or comma-like structures, evenly distributed and without orientation to the nucleus or lumen of the gland.The nucleoli first increase both in number and size within the nucleus, then migrate out into the cytoplasm, where they undergo further growth, and finally are dissolved and passed out into the lumen of the gland as liquid secretion.Small vacuoles appear near the periphery of the gland in early periods of activity.In glands active for forty-eight hours all stages of the secretory process may be seen.In glands active for longer periods only vacuoles are present which we interpret as the remains of the secretory inclusions. There is a progressive decrease in the nuclear content of the nucleus.Throughout the activity of the gland the Golgi apparatus changes little from the normal condition, both in form and distribution. As activity progresses, the bodies become smaller and slightly more dispersed. No relation of Golgi apparatus to the secretory phenomenon is apparent.
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  • 34
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    Journal of Morphology 47 (1929), S. 283-333 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The significance of the ultimobranchial body has been the object of a comparative study of the structure in twenty-four species of urodeles. In nineteen of these it has not hitherto been described.Caudal to the last branchial arch, it develops as a thickening and later as an outpushing from the ventral wall of the pharynx. Due to the growth mechanics of the region, it comes to lie obliquely to the pharynx, ventral to it, and dorsal or dorsolateral to the pericardial cavity in its anterior region. It persists throughout life as an epithelioid or epithelial structure, usually of irregular shape, frequently containing vesicles; in some cases it exhibits a considerable amount of secretory activity of variable quality. Except in Amphiuma and Necturus, where it is regularly paired, and in occasional instances in individuals of other species, where it occurs on both sides, it is usually present on the left side only. Its occurrence is constant in all of the species of urodeles for which it has been examined.It is variable in size, form, and position. This, together with the quite inconstant indication of secretory activity, marks it as a structure of little or no physiological significance. ‘Colloid’ is, however, present in some instances, and hence a comparison with the thyroid was considered.
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  • 35
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    Topics: Biology , Medicine
    Notes: During the past few years many functions have been assigned to the epididymis which have been supplementary to its rǒle in sperm storage and to its secretory activity. One of these, the suggestion that sperm attain full maturity and are strengthened in consequence of some action of its secretion, has been reinvestigated as the first part of a study which is intended to include other aspects of the problem.No evidence in support of the theory was obtained from a series of experiments on various mammals representing a repetition and extension of earlier work. On the contrary, the strengthening and attainment of full spermatozoon maturity would seem to be the outcome of changes which are inherent in the sperm themselves. It is suggested, therefore, until other aspects of the subject can be reinvestigated, that the epididymis is simply a reservoir for sperm in which the processes of sperm development which start while they are still contained in the testis are free to continue because of the favorable environment present in the epididymis.
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  • 36
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    Topics: Biology , Medicine
    Notes: This study concerns the voice box or syrinx of the fowl in its possible relation to sex. Although neither hens nor capons crow, ovariotomized hens have been known to do so. It appears that a slight sexual dimporphism exists in the syrinx of certain fowls, and it was thought that a particular form might be essential to the act of crowing. However, no sexual dimorphism is apparent in the syrinx of the Brown Leghorn fowl.The syringeal structures of crowing birds (cocks and ovariotomized females) contain no features which cannot be demonstrated in normal females. Variations were found in the syrinx in this breed and in other breeds, but they had nothing to do with sex. There is a gradual ossification with increasing age and size. It may be concluded that there is no apparent reason why the female fowl should not crow, provided it had the instinct to do so properly developed. The sex hormones, if they act in voice production, must act entirely through the conditioning of the central nervous system.
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  • 37
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    Journal of Morphology 47 (1929), S. 531-553 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The external genitalia and the internal reproductive organs are described. Compound gonads, with a normal development of testicular and ovarian tissues, are found in the positions of the gonads of normal individuals. The genital ducts arise in both types of tissue and serve as common ducts.It is demonstrated microscopically that the male tissue has one and the female tissue two sex chromosomes. The diploid number (or nearly so) is present in both tissues. This is the constitution of the normal sexes.The origin of the gynandromorphs is accounted for by assuming either the theory of dispermy in the binucleated egg or chromosomal elimination.
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  • 38
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    Notes: The rate of locomotion in Amoeba proteus is dependent upon the nature of the substratum, the nature of the different divalent and monovalent cations present, and the ratio of the amount of the monovalent cations to the amount of calcium or strontium. Calcium is more efficient than strontium in antagonizing potassium. Magnesium and barium do not antagonize potassium. The four monovalent cations studied in antagonizing calcium are efficient in the following order: Na〉K〉Li〉Rb. Calcium and potassium are more effective against antagonistic cations when combined with chlorine than when combined with phosphate.
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  • 39
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Maturation of the male germ cells in the rat shows some slight modifications of the typical procedure in sex cells, a distinct and rather prolonged synapsis occurring before synizesis and a confused stage immediately after it. The clumping in synizesis is not extreme. In the mixed strain of rats both twenty-one and thirty-one tetrads appear in the late diakinesis. This procedure in the male resembles the maturation of the oocytes in only two points, the beginning of the process, the deutobroch nuclei, and the end of it when the haploid number of chromosomes take their places on the spindle.
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  • 40
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Following the isolation of sperm in the epididymides of male guinea-pigs by the separation of this organ from the testis, the animals were found to remain fertile for periods varying from twenty to thirty-five days, whether they were allowed to mate but once during this period, or five times during the period, or twenty times during the period. Motile sperm were found as long as fifty-nine days after the operation. It was concluded, on the basis of these data, that an aging process which sperm isolated in the epididymis undergo is more important than the number of matings (up to at least nine or ten) in the determination of the period during which sperm motility is retained and in the determination of the period during which fertilizing capacity is retained.If the behavior of sperm isolated in the epididymides can be taken as an indication of the processes of sperm development which occur normally, the epididymis would seem to be an organ in which sperm may at one time be attaining an optimal functional state and may at another time be aging and becoming incapable of functioning. The possible significance of this suggestion for the rǒle of the epididymis in sperm development and for the history of sperm after they leave the testis is discussed.
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  • 41
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    Journal of Morphology 48 (1929), S. 493-541 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cells of the blastoderm which are to form the serosa are two- to four-nucleate; the smaller cells of the embryonic rudiment, uninucleate. The band-like embryonic rudiment encircles the yolk at the equator of the egg. The amnion does not begin to form until after the serosa completely covers embryo and yolk. The epithelium of the midgut arises from cells situated at the tips of stomodaeum and proctodaeum. These cells, though not differentiated from adjacent ectoderm at the time of the invagination, are nevertheless interpreted as part of the preprimordium of the endoderm. In the eighty-four-hour stage a fold of amnion grows over the dorsal side of the embryo, entirely covering it in the course of the next few hours. A portion of the amnion thus forms the dorsal wall of the embryo. At the completion of the amnion the embryo rotates so that its ventral side is directed toward the egg center. The amnion raptures just before the larva begins to feed on the yolk which still remains around it. The serosa is consumed before hatching, which takes place about five and one-half days after deposition.
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  • 42
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    Journal of Morphology 41 (1925), S. 239-265 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This paper reports experiments with Fundulus heteroclitus to modify larval development by means of ultraviolet radiation. Eggs were exposed to radiation at various intervals after fertilization for varying periods of time. The results accord with previous work on fish teratology, and the developmental types obtained are essentially similar to those produced by chemicals, cold, and hybridization. The deviations from normal development occur in the same body regions as do those in other vertebrates whose early development has been modified by the action of radiation (x-rays, radium, etc.).The results of these experiments indicate that there is a non-specificity in susceptibility relations with the production of similar types of monsters for widely different reagents. That these eggs are differentially susceptible to the action of ultraviolet radiation is indicated by the fact that those regions which have the highest metabolic activity when an inhibiting influence is active are the ones most generally affected. Thus modifications of the nervous system, sense organs, circulatory system, tail region, and body axis result, respectively, in the production of varying degrees of cyclopia, inadequate circulation, short, stubby or bent, non-motile tails (some bifid), and anterior twinning.As these modifications may be produced by applying inhibiting influences during the first few minutes after fertilization, it is evident that there is in the egg at this stage some constitutional or physiological basis which determines early differences in susceptibility of its various parts.
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  • 43
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    Journal of Morphology 40 (1925), S. 235-259 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A study of the peripheral nerves of the earthworm shows that one pair of nerve trunks arise from the lateral regions of the cerebral ganglion, one pair from near the lateral, and two pairs from the ventral region of the circumpharyngeal connectives. These supply, in the order given, the prostomium, segment 1 and segment 2. Segment 3 and each succeeding segment, except the last, are supplied with three pairs of nerve trunks which arise from the nerve cord in the segment concerned. None of the nerve trunks of the body was observed to send branches to more than one segment, which shows that they are segmental in origin and distribution.The anterior portion of the central nervous system has undergone caudal migration and modification of its ganglia. Some of the nerve trunks have disappeared through atrophy, while others have become fused at their bases.A subepidermal nerve plexus is found at the base of the epidermal cells from which nerve fibers pass to the internal nerve net and to the central nervous system. This plexus also sends intercellular and intracellular fibers into the epidermis.The so-called sense cells of the epidermis appear to be the cell bodies of sensory neurones which send nerve fibers to the ganglia of the central nervous system, where they form synaptic connections with other neurones.There is an enteric nerve plexus in the wall of the alimentary canal which is directly connected to the circumpharyngeal connectives.
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  • 44
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    Journal of Morphology 40 (1925), S. 341-416 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Astylosternus robustus has greatly reduced lungs. All Amphibia exhibiting such a reduction have their epidermis either penetrated by capillaries or thinned to facilitate greater cutaneous respiration. The epidermis of both sexes of A. robustus is penetrated by capillaries. The «hairs» of the adult male are merely extensions of this vasculated epidermis to compensate for the greater muscularity, size, and activity of this sex.The digits of terrestrial urodeles do not serve as special centers of cutaneous respiration. Digital sinuses are present in all urodeles. The abdominal and femoral tubercles of arboreal and some terrestrial frogs may function greatly in respiration, for their epidermis is penetrated by capillaries.All urodeles and frogs having well-developed and frequently emptied lungs possess a functionally complete auricular septum and a spiral valve. Injection experiments demonstrate a complete separation of arterial and venous blood in living specimens. A reduction of the lungs conditions a reduction of the left auricle, but disuse causes no change in size. A great decrease of the lungs, or even a disuse of them, conditions a fenestration of the auricular septum and a loss of the spiral valve. The spiral valve is formed by a backward growth of one of the synangial valves and is not homologous with the accessory valves of dipnoans. Lungless salamanders have no left auricle and no spiral valve.
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  • 45
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    Journal of Morphology 40 (1925) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 46
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    Journal of Morphology 41 (1925), S. ii 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 47
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    Topics: Biology , Medicine
    Notes: A special type of cell, called ‘Lichtzellen’ by Hesse, was found in the photosensitive regions of the earthworm. Wherever the epidermis is most sensitive to light these cells are most abundant. They are found in the epidermis of all segments of the body and also in nerve enlargements of the prostomium and the caudal segment, but the intersegmental and ventral regions of the different segments, except the more distal ones, contain none of them. They are supplied by nerves and each contains a characteristic inner structure, the optic organelle, composed of a large central hyaline structure, the lens, which is surrounded by a dense network of nerve fibrillae, the retinella. In hanging drops the lens was found to focus light in the region of the retinella irrespective of the direction of the rays.These cells are similar in structure and function to the visual cells in leeches. Available data indicate that these cells function as photoreceptors and that the fibrillae of the retinella are the direct receptors of light stimuli.Pigment is not associated with the photoreceptors in a way that suggests direct functional relationship, but there is a subepidermal pigment layer through which pinhole windows admit light along the path of nerves to each of the nerve enlargements containing photoreceptor cells. These windows open in such directions as to determine the direction of withdrawal of the worm.
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  • 48
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    Journal of Morphology 41 (1925), S. 95-157 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This paper represents the third part of a monograph on the development of the lymphatics and lymph hearts of Anura. In this part are considered the origin and development of the subvertebral, lateral, and dorsal lymphatics of the trunk, the caudal lymphatics, and the posterior lymph hearts.The chief lymphatic trunks arise along the veins from a series of small, spindle-shaped, and discontinuous anlagen which subsequently coalesce and thus establish continuity with one another. Lymph plexuses are formed from which emerge the main secondary lymph channels. After such trunks are laid down as indicated, smaller branches and capillaries spring from them by sprouting or extension, due to their actively proliferating endothelium.The posterior lymph hearts ‘crystallize,’ so to speak, from localized areas of the lateral lymphatic plexus. During its reorganization and differentiation, it temporarily separates from the surrounding lymph plexus. Other genetic details, such as the formation of its valves, histogenesis of its walls, number of hearts laid down, etc., are also considered. Twenty-two figures (microphotographs and reconstructions) accompany the text.
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  • 49
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In general, the changes of locomotor rate of Amoeba under the influence of temperature are similar to the effects of temperature in other biological processes.The rate increases with rising and decreases with falling temperatures within a certain temperature range, this being, for the forms investigated, the interval between 6° and 23.5°C. In some individuals, however, these limits were found to be wider, extending from 2° to 26°. There is an average optimum at about 23.5°, beyond which an increase in temperature is followed by a decrease in rate. The immediate response of Amoeba to a change of temperature is variable, due, as was shown in a previous paper, to the fact that locomotor-rate changes are rhythmic. The effect of a change of temperature is thus conditioned not only by the enviornmental factors, but also by the phases of the locomotor rhythm. This fact also makes it difficult to establish the exact quantitative relationship which exists between a change of temperature and the locomotor rate - an aspect which will be developed in a future paper.
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  • 50
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    Journal of Morphology 40 (1925), S. 191-233 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hermaphroditic gonads of Rhabdites elegans can be distinguished in early developmental stages, but oogonia cannot be told from spermatogonia. Certain peculiar males show developmental processes approaching those of hermaphrodites. More eggs are produced by a hermaphrodite than there are sperm with which to fertilize them. Such exhausted hermaphrodites may be mated with males and produce fertile eggs, but convincing evidence of cross-fertilization is lacking. Unmated females of Diplogaster aerivora live longer than mated ones, but no difference was found in the males. Sexual instinct seems to be lacking in males of R. dolichura and R. elegans.Hermaphrodites mated with males produce many more male offspring than by self-fertilization, but these do not exhibit sexual instincts. Females produced by cross-fertilization are not pure females, nor have they been found at all in R. elegans or R. dolichura. After fertilization in these two species, both polar bodies are formed and the pronuclei fuse. Sex is determined by the form of sperm.
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  • 51
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    Journal of Morphology 40 (1925), S. 261-297 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The parasynaptic union of chromosomes associated with the formation of a karyosphere is demonstrated in the spermatocytes of Phanaeus.The twelve V-shaped leptotene threads are polarized with their apices embedded in one plasmosome-like body, their distal ends in another (primary and secondary caps, respectively), and undergo a conjugation of the parasynaptic type. The distal ends of the chromosomes are freed from their attachment in the secondary cap. The six pachytene loops retain their polarized configuration.The basichromatin of the pachytene chromosomes appears ultimately to be withdrawn to form the karyosphere comprising six chromatic bodies within an oxychromatic «plasmosome,» the latter probably derived from the primary cap. The two caps are believed to arise from the chromosomes. The primary cap apparently becomes incorporated again in the chromosomes; the secondary cap, together with linin remnants of the pachytene chromosomes, disintegrates in the nucleus as residual chromatin.In the dissolution of the karyosphere six ring-shaped tetrads emerge arranged in a temporarily connected chain, giving under certain conditions, the misleading impression of twelve components arranged end to end. The entire content of the karyosphere appears to be employed in the formation of the chromosomes; no visible plasmosome remains.
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  • 52
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    Journal of Morphology 40 (1925), S. 299-340 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The course of digestion of an essentially protein food (beef liver) was followed by histological and histochemical methods in Planaria dorotocephala. After an average fasting period of two weeks, the planarians were fed and examined at close time intervals, ranging from one minute to eighty-seven days after feeding. Liver particles are engulfed by the phagocytic cells of the intestine into food vacuoles, where a series of digestive changes occur. The liver particles coalesce into a granular spherule which gradually condenses into a homogeneous and chromatophilic spherule. As the spherule transforms from a granular to a homogeneous condition, it exhibits a gradually increasing affinity for stains, an intensified protein reaction, and a gradual diminution in size. These spherules are protein, since they give positive xanthoproteic and Millon reactions.The homogeneous protein spherules gradually disappear, beginning at about twelve hours after feeding and continuing until the fifth day. During this period fat-droplets form, gradually increasing at the expense of the protein spherules. The protein spherules apparently dissolve into the cytoplasm, furnishing intermediate chemical compounds (tests indicate that a carbohydrate stage does not intervene) from which fat is synthesized. It thus appears that the fat is formed from the protein spherules. The fat thus formed is stored in the phagocytic cells, and remains unchanged in quantity for a fasting period lasting six weeks, after which it gradually diminishes. During the engulfment of the food, the fat decreases markedly, apparently furnishing energy for phagocytic activities. A few of the protein spherules may persist as «protein reserve».
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  • 53
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    Journal of Morphology 41 (1925), S. 267-281 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Of the eggs laid by Fasciolaria about 1 per cent develop into veligers, about 2 per cent more undergo a few cleavage divisions, and about 97 per cent do not divide at all. The 99 per cent that fail to develop normally are ‘swallowed’ by the veligers. This study concerns itself chiefly with the ova that do not divide. Notes on normal development and on the ova that undergo atypical cleavage are included.All of the ova are found to be typical when passed from the ovary. To each ovum one to several sperms become attached at the vegetal pole in the region of a mass of undifferentiated protoplasm - the ‘polar mass.’ A fertilization cone forms in each ovum and a fertilization membrane. In typical development a yolk lobe is formed, the sperm enters in the usual way, and fertilization is completed as in many other mollusks. In 97 per cent of the ova the yolk lobe is not formed and the sperm does not enter. In these cases the wall of the egg nucleus remains intact a long time. The nucleus itself and the ‘polar mass’ sink into the egg and meet at the center. Then the nuclear wall disappears and an atypical diaster is formed. However, cleavage is not begun and the chromosomes form vesicles that remain near the center of the ovum until it is ‘swallowed’ and digested by the veliger.
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    Journal of Morphology 40 (1925) 
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    Journal of Morphology 40 (1925), S. 1-109 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Much more information than is available at present must be obtained before the phylogeny of the muscular system in the Teleostei can be worked out satisfactorily. As a step toward the solution of this problem, the present study gives a detailed description of twenty cranial muscles in a number of species belonging to three groups of cypriniform fishes: the Cyprinoids, Cobitoids, and Siluroids.Particular attention is given to the morphological relations of the following muscles: adductor mandibulae, geniohyoideus, levator arcus branchialis, adductor arcus palatini, retractor branchialis dorsalis, interarcualis dorsalis and the trapezius.Comparison is made between corresponding muscles in different members of the three groups of fishes, and various homologies are pointed out between muscles in cypriniform and those in other fishes, especially Amia, Scomber, Perca and Esox.
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    Journal of Morphology 40 (1925), S. 111-167 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microscopic examination of both living and stained forms indicates that the so-called ‘multivacuolate’ and ‘amicronucleate races of Paramecium caudatum’ belong to the species Paramecium multimicronucleata. The published accounts of the morphology and cultural characteristics of the various forms are compared and provide additional evidence. A more complete description is given, including distinctive characters previously omitted.The conjugation process of P. multimicronucleata in general resembles that of P. caudatum, most of the differences being due to the presence of four micronuclei instead of one. These each divide twice with characteristic figures, twelve of the sixteen daughter nuclei then degenerating. One of the remaining four splits to form a single pair of functional pronuclei in each cell. The two migratory pronuclei interchange as in P. caudatum. The synkaryon divides three times, and probably seven of these nuclei degenerate. The remaining one apparently undergoes two divisions. In most cases by six to eight hours after the conjugants separate two micronuclei of the four thus produced form enlarged macronuclear anlagen, the other two remaining as micronuclei. Each anlage divides within the next two or four hours, producing four macronuclear and four micronuclear analagen. Two fissions, each preceded by a division of the micronuclei, restore vegetative conditions.The process described above is compared with conjugation in P. bursaria, P. caudatum, and P. putrinum.
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    Journal of Morphology 40 (1925), S. 169-189 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A review of the literature of the gastric glands of reptiles shows marked differences of opinion concerning the nature and character of the cells composing the gastric glands.The present investigation of the gastric glands of the alligator has been carried out by histochemical and physiological experiments, rather than by the usual morphological methods, but the results can be stated both in terms of structure and function.1The surface epithelial cells and those lining the gland pits in the fundic, cardiac, and pyloric regions of the stomach of the alligator are not strictly comparable functionally to the mucous cells of mammals.2The cells composing the necks and bodies of the glands in the fundus, according to histochemical reactions, are parietal cells.3Certain cells lying beneath the columnar epithelial cells lining the lumen of the stomach and the foveolae react to one method of fixation and staining as do the chief cells of mammalian material.4The results of the experiment with the vital dye, neutral red, show that the cells of the gastric pits, the necks and bodies of the glands in the fundus of the alligator's stomach, react identically as those of the mammalian stomach.5The gastric juice of the alligator is acid to litmus-paper and contains a free mineral acid.
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    Journal of Morphology 40 (1925), S. 417-459 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The development and cytology of cartilage and bone in the limbs from the fifty-second hour of incubation to the first day after hatching are described.The chief points of interest arising from this study are: (1) Chondroblasts when liberated during cartilage resorption die and disintegrate. (2) All the long bones possess structures which, though not undergoing independent ossification, are homologous with the mammalian epiphyses. (3) Endochondral ossification occurs only in the epiphyses and ends of the diaphyses. (4) The marrow cavity contains multinucleated giant cells which appear to be formed by the fusion of degenerating cells.
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    Journal of Morphology 40 (1925), S. 461-477 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Total splenectomy in the frog means practically complete extirpation of the erythropoietic apparatus. Out of sixty-four operations it was followed by: 1Stimulation of erythropoiesis in the kidney (mesonephros), 35 to 46 per cent.2Stimulation of erythropoiesis in the fat-bodies, 5 per cent; lymphocytopoiesis, 8 per cent.3Stimulation of erythropoiesis in the bone-marrow, 11 per cent.4Formation of a new spleen, 8 per cent.5No perceptible erythropoletic stimulation, 24 per cent. The latter leads to a condition of anemia followed by death within about sixty days.Partial splenectomy leads to spleen regeneration.The life tenure of the frog erythrocyte is approximately 100 days.Total splenectomy experiments indicate that the chief function of the spleen in the frog inheres largely in its lymphocyte content and in the transformation of lymphocytes into erythrocytes.
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    Journal of Morphology 40 (1925), S. 517-557 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An early segregation of germ cells and migration through a germ track into the gonad does not occur in the albino rat. The germ cells are produced only from the peritoneum of the genital region and their earliest formation is coincident with the thickening of the coelomic epithelium to form the genital ridge. This takes place eleven days after insemination in embryos of approximately 18 somites. Germ cells continue to form from the peritoneum during the early development of the gonad. The peritoneum of this region also produces mesenchyme, smaller cells of the gonads, and the germinal epithelium.The argument for the specific character of the germ cells in vertebrates and their continuity from the egg is based largely upon assumption, and not upon substantial observations, and must be discarded. Germ-cell origin is a problem of cellular differentiatio, and not of early segregation.
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  • 62
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    Notes: Systems of fiber-like structures traverse ciliated cells and epithelia in a way to suggest conduction paths for stimuli for coordinating and regulating ciliary movement, and results of microdissection support such an interpretation.Some fibers of the systems in epithelia of the gill terminate on the nuclear membrane, some between nuclei in the basal cytoplasm.The observed double refringency of the fibrillar apparatus places it in the category of irritable and contractile substance.Bipolar cells and nerve-like fibers lie immediately below and parallel with ciliated epithelia of the gill, but direct connections of these fibers with the intracellular system, although suspected, was not demonstrated.Ciliated epithelia of the gill are syncytial in character in the direction of the beat of cilia.Interciliary structures occur in the cuticular portion of ciliated epithelia which are interpreted to function in taking up strains in the gelled cytoplasm caused by the beat of cilia.Cilia occur in pairs in epithelia of the gill, and members of pairs are fused at their distal ends.Each cilium, as seen in dark-field illumination, pierces its basal body, and the fiber that is a continuation of the cilium divides immediately below the basal body into two; one diverging to the right, one to the left.Each cilium is composed of many delicate fibrillae.
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    Journal of Morphology 40 (1925), S. 559-573 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: One of the most perfect “lock and key” adaptations known, the external reproductive organs in spiders are subject to considerable variations. Two extreme types of female reproductive organs are described in detail, and these in turn compared with the European species of the same genus. Thus it is shown that the reproductive system in the same genus of spiders may exhibit great differences in structure while all other characters remain more or less the same, the natural conclusion being that caution should be exercised in creating new or splitting up old genera on the basis of characters of the external reproductive system. The same applies to the male.Two extreme types of palpi are described and then compared with the palpi of the European species. In the case of the male, however, the differences seem to be of a less profound nature.
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    Journal of Morphology 41 (1925) 
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    Journal of Morphology 40 (1925), S. 575-592 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The shedding of epidermis in this species, as in most amphibia, is periodic. A single outer layer of cells separates nearly simultaneously over the whole body; two hours to ten days may be required for the process. In the first three months of laboratory existence the average frequency of molting was 9 per cent. In nature the frequency was only 2 per cent; this increased during the life in confinement in five months up to 25 per cent. Death in the laboratory was usually preceded by numerous molts.The time of a molt is not normally determined by any one of a number of ordinary external conditions which were modified; but nevertheless several means were found of inducing molting. These were, in order of their efficacy: bandaging with gauze on body or tail, wrapping the body with thread, stitching the body wall, applying hot water, and transplanting skin. Molting frequencies up to 98 per cent may thus be obtained.Since molting is periodic and simultaneous, it is evidently coordinated. Experimental induction requires a latent period of six to ninety-six hours, depending upon the stimulus. Recent skin grafts molt with the host, so that the coordination is probably chemical. The primary significance of molting in all groups of animals is possibly the getting rid of unautolyzable waste materials, i.e., worn epithelial structures.
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    Notes: The primordial germ cells of urodeles and anurans can occupy comparable positions in the embryo only in very early stages of development, if at all. When first recognizable as reproductive elements, their situation in the two amphibian orders is quite strikingly different.The germ cells first become recognizable in urodeles in the medial edge of the lateral mesoderm; there is no evidence that their position previous to this time is other than mesodermal. In the anura they are entodermal in association during early development; they are finally separated from the dorsal portion of the roof of the archenteron to form a single axial germ-cell cord. No amphibian species studied showed a mode of origin different from that characteristic of its order.The so-called migration of the amphibian germ cells to their definitive situation is interpreted as the result of growth shiftings of related parts. Germ cells do not migrate independently through the tissues. Aberrant or ectopic cells for the most part result from failures or faulty correlations of growth processes. Certain aberrant cells of the caudal portion of the body in urodeles indicate the inclusion of sex elements in the ventral as well as in the lateral mesoderm.
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    Notes: The musculature of the ophidian head is a fairly complex structure, due to the specialization of the skull and the peculiar movements and motions made possible by the high degree of streptostylism.This musculature has been derived from the lacertilian type by a splitting and a shifting of the original elemental muscle masses of this group. Most of the muscles are clearly homologous with those of the Lacertilia. Some cannot be homologized by a study of the mature forms.The greatest differences lie in the separation of the muscle masses in the ophidia, as compared with the undivided masses in the Lacertilia. Since the jaw movements of the Lacertilia are rather simple, there is no need for any subdivision or splitting of the jaw muscles, but with the complex movements of the ophidia this becomes necessary.A few of the ophidian muscles appear to be neomorphs, as no key to their origin could be determined by a comparison with lacertilians.
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    Journal of Morphology 41 (1925), S. 191-216 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Painted turtles, gopher tortoises, and terrapins were fed on various mixtures of sand, salts, dextrin, casein, cod-liver oil, wheat, eggs, lettuce, and meal worms. Each individual was weighted weekly for about a year and then killed for analysis, the water, ash, nitrogen, and fat being determined. Some individuals increased in weight as much as 75 per cent, others lost weight. Judged by growth and chemical analyses, the food requirements of chelonians, as representative poikilothermal vertebrates toward nutritive substances (including vitamines) are similar to those of homoiothermal animals.
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    Notes: The effect of removal of the liver has been noted in fishes, frogs, and turtles. As in the higher vertebrates, removal of the liver produced a fall in blood sugar and a loss in muscular tone. The lower vertebrates failed to respond to intravenous injections of glucose, as do the birds and mammals. They also fail to respond to maltose or levulose. The liver maintained the blood-sugar level in the lower vertebrates, which is necessary for the maintenance of life.The mechanism of carbohydrate metabolism in the lower vertebrates may be different from that in the higher ones, in that glucose, when injected intravenously, apparently exercises a progressively less beneficial effect on the characteristic hypoglycemic condition which follows the removal of the liver of mammals and cold-blooded vertebrates.
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    Journal of Morphology 41 (1925), S. 217-237 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The female reproductive system comprises the ovaries, oviducts, vagina, spermatheca, and spermathecal gland. No colleterial glands are present. The ovaries are paired and each is composed of about eighteen ovarian tubules terminating in a cup-shaped modification of the oviduct - the egg calyx. The epithelial lining of the oviduct is glandular. The spermatheca, a bladder-shaped sac situated dorsal to and between the two ovaries, opens into the vagina near the exterior and is filled with spermatozoa after copulation. Spermatozoa have not been found in any other part of the female. Dorsal to the spermatheca and lying against it is the spermathecal gland. It is connected with the spermatheca by an elongate spermathecal duct.The male reproductive system comprises the testes, vas deferens, accessory glands, and the penis. Each testis is composed of eight to ten cone-shaped follicles, which are composed of a number of cysts so arranged as to appear segmented. All of the cells of each cyst are in approximately the same stage of development. No seminal receptacle is present. Each follicle is connected with the vas deferens by an efferent tube. The paired vas deferens opens into the sac-like intromittant organ or penis. The terminal portion of the penis may be everted, and when everted is conical in shape. At its base is a thin chitinous collar, the margins of which are thickened, forming ridges.
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