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  • Articles  (14)
  • Oyster
  • Springer  (8)
  • Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu  (6)
  • American Chemical Society
  • American Physical Society (APS)
  • Copernicus Publications
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  • Articles  (14)
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  • 1
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: Nutrient and metabolic fluxes on oyster reefs
    Description: This dataset contains the nutrient and metabolic fluxes data that was published in Jackson et al. (2018) that compared two methods of measuring nutrient and metabolic fluxes on restored oyster reefs: incubations including intact segments of oyster reef and incubations containing oyster clumps without underlying sediments. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/870783
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1427019
    Keywords: Oyster ; Biogeochemistry ; Oyster restoration ; Denitrification
    Repository Name: Woods Hole Open Access Server
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  • 2
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2022-10-31
    Description: Dataset: Oyster mortality
    Description: Database of oyster mortality based on body size treatment For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/804502
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1652320, Australian Research Council (ARC) FT140100322
    Keywords: Oyster ; Intra-specific ; Phenotypic diversity ; Predation ; Survivorship
    Repository Name: Woods Hole Open Access Server
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  • 3
    Publication Date: 2023-01-11
    Description: Dataset: Results of risk-addition experiment (adult oyster condition index)
    Description: The eastern oyster (Crassostrea virginica) is a foundation species in northeast Florida estuaries, including the Guana Tolomato Matanzas National Estuarine Research Reserve (GTMNERR), where intertidal reefs are extensive. Estuarine research to assess sustainability and oyster population, plus various monitoring studies and oyster reef restoration projects have been undertaken, with an additional focus on testing theory regarding the effects of predation risk in the natural environment. As part of a study that manipulatively “pressed” risk cues onto oyster prey, a field experiment was conducted on oyster reefs in the Guana Tolomato Matanzas National Estuarine Research Reserve (Ponte Vedra Beach, Florida) from June to November 2012. Three areas within the southern areas of the GTM NERR (south of Matanzas inlet) were used in the experiment: Summer Island North (SIN), Marine Land (ML) and Pellicer Flats (PF). The SIN site occurred closest to the inlet (farthest from freshwater input), the PF site occurred farthest from inlet and closest to freshwater input, while the ML site occurs between the inlet and the freshwater input. Oyster survival, growth and recruitment were checked monthly. At the mid point and conclusion of the experiment, individual oysters were also destructively sampled to quantify differences in oyster traits (shell versus tissue mass) as a function of experimental treatment and location. This submission concerns the condition index of adult oysters. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/885078
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1736943, NSF Division of Ocean Sciences (NSF OCE) OCE-1820540
    Keywords: Oyster ; phenotype ; Predation risk ; fitness ; nonconsumptive effect
    Repository Name: Woods Hole Open Access Server
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  • 4
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2023-02-28
    Description: Dataset: Seawater and Extrapallial fluid Concentration
    Description: Trace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents the phenotypic and molecular responses in the extrapallial fluid in the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888887
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1635423
    Keywords: Oyster ; extrapallial fluid ; trace elements ; ocean acidification
    Repository Name: Woods Hole Open Access Server
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  • 5
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2023-02-28
    Description: Dataset: Shell Concentrations
    Description: Trace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents shell Concentrations of the adult eastern oyster (Crassostrea virginica) exposed to experimental ocean acidification (OA) over 80 days. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888902
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1635423
    Keywords: Oyster ; extrapallial fluid ; trace elements ; ocean acidification
    Repository Name: Woods Hole Open Access Server
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  • 6
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    Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu
    Publication Date: 2023-02-28
    Description: Dataset: Molar Ratios
    Description: Trace, minor, and major element data from adult Eastern oyster ocean acidification exposure experiments were conducted at the Ries Lab at the Northeastern University Marine Science Center on samples from Plum Island Sound in 2017. This dataset represents molar ratio data that were processed using R, using the graphical interface RStudio and then converted to molar ratios to calcium. For a complete list of measurements, refer to the full dataset description in the supplemental file 'Dataset_description.pdf'. The most current version of this dataset is available at: https://www.bco-dmo.org/dataset/888911
    Description: NSF Division of Ocean Sciences (NSF OCE) OCE-1635423
    Keywords: Oyster ; extrapallial fluid ; trace elements ; ocean acidification
    Repository Name: Woods Hole Open Access Server
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  • 7
    ISSN: 1476-5535
    Keywords: Vibrio vulnificus ; Oyster ; Monoclonal antibody ; Most probable number ; Enzyme immunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Oysters, suspended particulate matter (SPM), sediment and seawater samples were collected from West Galveston Bay, Texas over a 16-month period and analyzed for the presence ofVibrio vulnificus, a naturally-occurring human marine pathogen. Detection and enumeration ofV. vulnificus was performed using a species-specific monoclonal antibody (mAb FRBT37) in an enzyme immunoassay (EIA)-most probable number (MPN) procedure capable of detecting as few as 2000 target organisms.V. vulnificus was not detected in seawater, oyster or SPM samples during the cold weather months, but was detected at low levels in several sediment samples during this time period. Increased levels of the organism were first observed in early spring in the sediment, and then in SPM and oysters. The major increase inV. vulnificus occurred only after the seawater temperature had increased above 20°C and the winter-spring rainfall had lowered the salinity below 16‰. The highestV. vulnificus levels at each site were associated with suspended particulate matter. These results are consistent with the hypothesis that (1)V. vulnificus over-winters in a floc zone present at the sediment-water interface, (2) is resuspended into the water column in early spring following changes in climatic conditions, (3) colonizes the surfaces of zooplankton which are also blooming during early spring and (4) are ingested by oysters during their normal feeding process.
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  • 8
    ISSN: 1572-8773
    Keywords: Copper ; Silver ; Zinc ; Oyster ; Salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The accumulation and physico-chemical forms of metals were determined by atomic absorption spectrometry in the whole soft tissues of oysters, by histological and microanalytical techniques in tissue sections, by gel permeation chromatography of soft tissue homogenates. Oysters were reared according to four types of experimental conditions: exposed to silver (20 μg Ag/l) or unexposed, in sea water (33‰) or brackish water (8‰). Copper, zinc and silver accumulation in oysters are inversely related to salinity. Amoebocytes, which play a key role in accumulating copper and zinc in natural sea water, are able to sequester an important part of added silver as Ag2S. In brackish water an increase of the number of amoebocytes may be considered as responsible for the enhancement of Cu and Zn concentrations in the whole soft tissues. In such conditions, additional silver is concentrated in these cells rather than in the basement membranes which are target structures for Ag2S accumulation in sea water. If the global fluctuations of metal concentrations in the soft tissues are ascribable mainly to changes at the histological level, the fate of metals in the soluble fraction must not be neglected since the speciation of metals influences their toxicity. The freshening of sea water induced a change in the distribution of cytosolic silver and zinc but in no case were the molecular masses of compounds associated with each metal perfectly identical. From these results, it is concluded that the accumulation of silver by oysters is not mediated by the same mechanisms as those for copper and zinc.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Environmental geology 39 (2000), S. 1216-1226 
    ISSN: 1432-0495
    Keywords: Key words Bioaccumulation ; Heavy metals ; Oyster ; Sediment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Oysters and sediment have been collected from most major US Gulf of Mexico bays and estuaries each year since 1986. Selected samples of oyster soft tissue, shell and sediments were analyzed for Cd, Cr, Cu, Fe, Mn, Pb, and Zn for this study. Concentrations varied considerably from place to place but ratios of metals remained relatively constant. Cu and Zn are greatly enriched in oyster tissues, which is related to their physiological function. Cd is enriched in oyster shell because of the easy substitution between Cd and Ca. The concentrations of Pb and Cr in oysters are significantly lower than that in sediment, suggesting a good discrimination against these metals by oysters. Metal variations are a result of both nature and human activity.
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  • 10
    ISSN: 1432-0878
    Keywords: Leucocytes ; Invertebrates ; Oyster ; Ultrastructure ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of oyster leucocytes resembles to a great extent, that of typical eucaryotic cells. Organelles which have been described for the first time in this report are light granules, dense granules, protocentriole and X structure. Light microscopy reveals two morphological types of oyster leucocytes: agranular and granular. Based upon nuclear morphology and cytoplasmic compositions revealed in electron microscopy, at least three types of agranular and one type of granular cells are recognized. In the Giemsa-stained preparations, granular leucocytes exhibit three distinct types of cytoplasmic granules: refractile, dark blue, and pink, which presumably correspond to light granules Type A, B, and C seen in the electron micrographs. A granular leucocyte may contain one or more types of granules. Cytochemical investigations show that oyster leucocytes contain at least three hydrolytic enzymes: non-specific esterases, acid, and alkaline phosphatase. The latter two enzymes constitute 63% of the enzyme activity detected. These intracellular enzymes may be associated with the light granules and/or lysosome-like bodies. It is also demonstrated that the granular leucocyte population is significantly higher (P〈0.001) in the oysters experimentally infected with Bacillus mycoides (72.19±4.71%) as contrasted with that of the controls (37.18±4.48%). Leucocytes in progressive stages of degeneration are also described.
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  • 11
    ISSN: 1432-0878
    Keywords: Mitochondria ; Paracrystals ; Prismatic cristae ; Oyster ; Myocardium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several types of unusual mitochondrial configurations were found in myocardial cells of the oyster Crassostrea virginica Gmelin. These mitochondria include, in order of frequency, prismatic cristae, filamentous paracrystals in honeycomb and herringbone configurations, and paracrystals composed of rows of electron dense particles. The long, parallel, evenly spaced prismatic cristae are square or rhomboidal in cross section. In the space between the prismatic cristae are rodlike structures (4–6 nm in diameter) that are regularly spaced about 12nm apart and appear to pass between adjacent cristae. Filamentous paracrystals are observed in slender, elongated mitochondria. The filament spacing and form of these paracrystals suggest that they are composed of the intercristal rods. Alternatively, filamentous paracrystals might be tangential sections of prismatic cristae and intercristal rods. Particulate paracrystals which consist of dense lines or rows of particles are the least frequent type of unusual configuration. The particles are triangular, possibly pyramidal, in shape; their bases are 10–12 nm thick and repeat in rows every 17–18 nm. There is a close association between particulate paracrystals and prismatic cristae plus intercristal rods. Although similar mitochondrial configurations have been associated with disease or altered metabolism in a number of species, we have found no such association in the oyster as yet.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 16 (1994), S. 67-72 
    ISSN: 1573-0603
    Keywords: Cell culture ; Crassostrea gigas ; Heart ; Molluscs ; Oyster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pacific oyster,Crassostrea gigas, is the most economically important specie to the world shellfish breeding. It is important to note that infectious diseases, particularly viruses, may be hazardous for theC. gigas live-stocks. The study of these viral diseases and the development of diagnosis method need the establishment of in vitro methods for viral multiplication. As no oyster cell line is available actually, we have developed a procedure for primary culture of heart cells which could enable to study molluscan viruses in vitro, and could also provide a diagnosis method based on the search of eventual cytopathogen viral effects. Cells fromC. gigas ventricle of heart were dissociated by trypsin-EDTA treatment and the mechanical action of a Dounce type homogeneizer. The cells were inoculated in previously poly-D-lysin coated flasks. The optimised culture medium was L-15 (Leibovitz) prepared three fold concentrated, then diluted half with sea water, this mixture was supplemented with 10% FCS and 5%C. gigas hemolymph. Different cell types could be identified by transmission electron microscopy analysis, as mostly cardiomyocytes, fibroblast-like cells and pigmented cells, but also haemocytes were present in the cultures.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Methods in cell science 21 (1999), S. 183-192 
    ISSN: 1573-0603
    Keywords: Cell culture ; Clam ; Mollusc ; Oyster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present study attempts to establish cell culture systems for the oyster, Crassostrea gigas Röding and the hard clam, Meretrix lusoria Thunberg. Treatment with collagenase was better than trypsin at dissociating mollusc tissue fragments for in vitro culture. Heart tissue of oyster and hard clam proved to be the most promising target tissue for the establishment of cell lines in vitro. Primary cultures of clam heart were established and successfully maintained for more than 5 months. Collagenase at a concentration of 100 μg/ml may enhance the growth of oyster and hard clam heart cell cultures in vitro.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 170 (2000), S. 21-26 
    ISSN: 1432-136X
    Keywords: Key words Amylase regulation ; Digestive enzymes ; Oyster ; Feeding stimulus ; Abbreviations bp base pair ; cDNA copy DNA ; CCK cholecystokinine ; EDTA ethylenediaminetetraacetic acid ; IGF insulin growth factor ; MOPS 3-[N-morpholino] propane sulfonic acid buffer ; PCR polymerase chain reaction ; pfu plaque forming unit ; SDS sodium dodecylsulfate ; SSC sodium citrate buffer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  A Crassostrea gigas digestive gland copy DNA (cDNA) library constructed in the λ phage ZapII (Stratagene, La Jola, USA) was screened with an amylase heterologous probe. To get access to the complete cDNA, a polymerase chain reaction extension was conducted using DNA extracted from the phages. The complete cDNA sequence is 1688 base pairs (EMBL = Y08370). The deduced protein sequence is 519 aminoacids long with a 19 aminoacid signal peptide. Similarity with Pectenmaximus amylase is 72%. A 3-day nutrition experiment with a cyclic algal food supply was carried out. Amylase enzyme activities and mRNAs were individually measured on five animals, nine times a day. Messenger RNAs were quantified by dot hybridization using the previously characterized cDNA as probe. Variation of amylase mRNA was observed, in relation with the level of activity of the enzyme. Coordinated changes in RNA and enzyme levels suggested a possible transcriptional regulation of amylase in C. gigas as in vertebrates.
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