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  • 04.01. Earth Interior  (1)
  • metabarcoding
  • Wiley  (2)
  • Annual Reviews
  • 2020-2024  (2)
  • 1980-1984
  • 1960-1964
  • 1935-1939
  • 2023  (2)
  • 1
    Publication Date: 2024-01-22
    Description: Quantifying the abundances of fungi is key to understanding natural variation in mycorrhi-zal communities in relation to plant ecophysiology and environmental heterogeneity. High-throughput metabarcoding approaches have transformed our ability to characterize and com-pare complex mycorrhizal communities. However, it remains unclear how well metabarcodingread counts correlate with actual read abundances in the sample, potentially limiting their useas a proxy for species abundances. Here, we use droplet digital PCR (ddPCR) to evaluate the reliability of ITS2 metabarcodingdata for quantitative assessments of mycorrhizal communities in the orchid speciesNeottiaovatasampled at multiple sites. We performed specific ddPCR assays for eight families oforchid mycorrhizal fungi and compared the results with read counts obtained from metabar-coding. Our results demonstrate a significant correlation between DNA copy numbers measured byddPCR assays and metabarcoding read counts of major mycorrhizal partners ofN. ovata,highlighting the usefulness of metabarcoding for quantifying the abundance of orchid mycor-rhizal fungi. Yet, the levels of correlation between the two methods and the numbers of falsezero values varied across fungal families, which warrants cautious evaluation of the reliabilityof low-abundance families. This study underscores the potential of metabarcoding data for more quantitative analysesof mycorrhizal communities and presents practical workflows for metabarcoding and ddPCRto achieve a more comprehensive understanding of orchid mycorrhizal communities
    Keywords: droplet digital PCR ; fungalquantification ; metabarcoding ; mycorrhizalfungi ; orchid mycorrhiza
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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  • 2
    Publication Date: 2023-02-21
    Description: The hinterland of the Cenozoic Northern Apennines fold-and-thrust belt exposes the metamorphic roots of the chain, vestiges of the subduction-related tectono-metamorphic evolution that led to the buildup of the Alpine orogeny in the Mediterranean region. Like in other peri-Mediterranean belts, the tectono-metamorphic evolution of the Palaeozoic continental basement in the Apennines is still poorly constrained, hampering the full understanding of their Alpine orogenic evolution. We report the first comprehensive tectono-metamorphic study of the low-grade metasedimentary (metapsammite/metapelite) succession of the Monti Romani Complex (MRC) that formed after Palaeozoic protoliths and constitutes the southernmost exposure of the metamorphic domain of the Northern Apennines. By integrating fieldwork with microstructural studies, Raman spectroscopy on carbonaceous material and thermodynamic modelling, we show that the MRC preserves a D1/M1 Alpine tectono-metamorphic evolution developed under HP–LT conditions (~1.0–1.1 GPa at T ~ 400°C) during a non-coaxial, top-to-the-NE, crustal shortening regime. Evidence for HP–LT metamorphism is generally cryptic within the MRC, dominated by graphite-bearing assemblages with the infrequent blastesis of muscovite ± chlorite ± chloritoid ± paragonite parageneses, equilibrated under cold palaeo-geothermal conditions (~10°C/km). Results of this study allow extending to the MRC the signature of subduction zone metamorphism already documented in the hinterland of the Apennine orogen, providing further evidence of the syn-orogenic ductile exhumation of the HP units in the Apennine belt. Finally, we discuss the possible role of fluid-mediated changes in the reactive bulk rock composition on mineral blastesis during progress of regional deformation and metamorphism at low-grade conditions.
    Description: Published
    Description: 919-953
    Description: 2TR. Ricostruzione e modellazione della struttura crostale
    Description: JCR Journal
    Keywords: Adria Paleozoic basement ; alpine orogeny ; chloritoid ; subduction metamorphism ; Northern Appennines ; 04.01. Earth Interior
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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