ALBERT

Beschreibung: Festivals are experiential products heavily depending on the recommendations of previous visitors. With the power of social media growing, understanding the antecedents of positive electronic word-of-mouth (eWOM) intentions of festival attendees is immensely beneficial for festival organizers to better promote their festivals and control negative publicity. However, there is still limited research regarding eWOM intentions in the festival context. Thus, this study aims to fill such a gap by investigating the relationships among festival attendees’ enjoyment seeking motivation, perceived value, visitor satisfaction, and eWOM intention in a local festival setting. Additionally, the moderating role of gender was tested as it is one of the most important demographic variables to show individual differences in behavioral intentions. The results of structural equation modeling showed a positive effect of enjoyment seeking motivation on perceived value, visitor satisfaction, and eWOM intention. Moreover, gender differences in eWOM intention and a full mediating effect of visitor satisfaction between perceived value and eWOM intention for female respondents were revealed. The findings of this study extend the existing festival literature and provide insights for strategically organizing and promoting festivals to generate more positive eWOM which can be utilized as an effective marketing tool and a feedback channel.

Beschreibung: As acidic deposition has decreased across Eastern North America, forest soils at some sites are beginning to show reversal of soil acidification. However, the degree of recovery appears to vary and is not fully explained by deposition declines alone. To assess if other site and soil factors can help to explain degree of recovery from acid deposition, soil resampling chemistry data (8- to 24-year time interval) from 23 sites in the United States and Canada, located across 25° longitude from Eastern Maine to Western Ontario, were explored. Site and soil factors included recovery years, sulfate (SO42−) deposition history, SO42− reduction rate, C horizon pH and exchangeable calcium (Ca), O and B horizon pH, base saturation, and exchangeable Ca and aluminum (Al) at the time of the initial sampling. We found that O and B horizons that were initially acidified to a greater degree showed greater recovery and B horizon recovery was further associated with an increase in recovery years and lower initial SO42− deposition. Forest soils that seemingly have low buffering capacity and a reduced potential for recovery have the resilience to recover from the effects of previous high levels of acidic deposition. This suggests, that predictions of where forest soils acidification reversal will occur across the landscape should be refined to acknowledge the importance of upper soil profile horizon chemistry rather than the more traditional approach using only parent material characteristics.

Beschreibung: Ice accretion is a phenomenon whereby super-cooled water droplets impinge and accrete on wall surfaces. It is well known that the icing may cause severe accidents via the deformation of airfoil shape and the shedding of the growing adhered ice. To prevent ice accretion, electro-thermal heaters have recently been implemented as a de- and anti-icing device for aircraft wings. In this study, an icing simulation method for a two-dimensional airfoil with a heating surface was developed by modifying the extended Messinger model. The main modification is the computation of heat transfer from the airfoil wall and the run-back water temperature achieved by the heater. A numerical simulation is conducted based on an Euler–Lagrange method: a flow field around the airfoil is computed by an Eulerian method and droplet trajectories are computed by a Lagrangian method. The wall temperature distribution was validated by experiment. The results of the numerical and practical experiments were in reasonable agreement. The ice shape and aerodynamic performance of a NACA 0012 airfoil with a heater on the leading-edge surface were computed. The heating area changed from 1% to 10% of the chord length with a four-degree angle of attack. The simulation results reveal that the lift coefficient varies significantly with the heating area: when the heating area was 1.0% of the chord length, the lift coefficient was improved by up to 15%, owing to the flow separation instigated by the ice edge; increasing the heating area, the lift coefficient deteriorated, because the suction peak on the suction surface was attenuated by the ice formed. When the heating area exceeded 4.0% of the chord length, the lift coefficient recovered by up to 4%, because the large ice near the heater vanished. In contrast, the drag coefficient gradually decreased as the heating area increased. The present simulation method using the modified extended Messinger model is more suitable for de-icing simulations of both rime and glaze ice conditions, because it reproduces the thin ice layer formed behind the heater due to the runback phenomenon.

Beschreibung: Failures of marine propulsion components or systems can lead to serious consequences for a vessel, cargo and the people onboard a ship. These consequences can be financial losses, delay in delivery time or a threat to safety of the people onboard. This is why it is necessary to learn about marine propulsion failures in order to prevent worst-case scenarios. This paper aims to provide a review of experimental, analytical and numerical methods used in the failure analysis of ship propulsion systems. In order to achieve that, the main causes and failure mechanisms are described and summarized. Commonly used experimental, numerical and analytical tools for failure analysis are given. Most indicative case studies of ship failures describe where the origin of failure lies in the ship propulsion failures (i.e., shaft lines, crankshaft, bearings, foundations). In order to learn from such failures, a holistic engineering approach is inevitable. This paper tries to give suggestions to improve existing design procedures with a goal of producing more reliable propulsion systems and taking care of operational conditions.

Beschreibung: In this study, the environmental impacts of various alternative ship fuels for a coastal ferry were assessed by the life cycle assessment (LCA) analysis. The comparative study was performed with marine gas oil (MGO), natural gas, and hydrogen with various energy sources for a 12,000 gross tonne (GT) coastal ferry operating in the Republic of Korea (ROK). Considering the energy imports of ROK, i.e., MGO from Saudi Arabia and natural gas from Qatar, these countries were chosen to provide the MGO and the natural gas for the LCA. The hydrogen is considered to be produced by steam methane reforming (SMR) from natural gas with hard coal, nuclear energy, renewable energy, and electricity in the ROK model. The lifecycles of the fuels were analyzed in classifications of Well-to- Tank, Tank-to-Wake, and Well-to-Wake phases. The environmental impacts were provided in terms of global warming potential (GWP), acidification potential (AP), photochemical potential (POCP), eutrophication potential (EP), and particulate matter (PM). The results showed that MGO and natural gas cannot be used for ships to meet the International Maritime Organization’s (IMO) 2050 GHG regulation. Moreover, it was pointed out that the energy sources in SMR are important contributing factors to emission levels. The paper concludes with suggestions for a hydrogen application plan for ships from small, nearshore ships in order to truly achieve a ship with zero emissions based on the results of this study.

Beschreibung: We compared the catalytic effects of two polymers (soluble starch and apple pectin) on the flocculation of kaolinite suspension. Moreover, the relationship between the zeta potential value and the time when kaolin particle sedimentation occurred was verified, and the mechanism of flocculation was analyzed. Additionally, a constitutive model was proposed to simulate the non-ideal sedimentation of clay particles in an aqueous system under constant gravity. This model not only considers the inhomogeneity of the solute but also simulates the change in clay concentration with time during the deposition process. This model proposes a decay constant (α) and sedimentation coefficient (s). The model can also be used to calculate the instantaneous sedimentation rate of the clay suspensions at any time and any depth for the settling cylinder. These sedimentary characteristics were simulated by adopting the established deposition model. The results show that the model is capable of predicting the time required for the complete sedimentation of particles in the aqueous system, suggesting the feasibility of engineering wastewater treatment, site dredging, etc.

Beschreibung: Dense β-SiC coating with 3C-structure was utilized as a dry cold forging punch and core-die. Pure titanium T328H wires of industrial grade II were employed as a work material. No adhesion or galling of metallic titanium was detected on the contact interface between this β-SiC die and titanium work, even after this continuous forging process, up to a reduction in thickness by 70%. SEM (Scanning Electron Microscopy) and EDX (Electron Dispersive X-ray spectroscopy) were utilized to analyze this contact interface. A very thin titanium oxide layer was in situ formed in the radial direction from the center of the contact interface. Isolated carbon from β-SiC agglomerated and distributed in dots at the center of the initial contact interface. Raman spectroscopy was utilized, yielding the discovery that this carbon is unbound as a free carbon or not bound in SiC or TiC and that intermediate titanium oxides are formed with TiO2 as a tribofilm.

Beschreibung: The manufacturing of parts from nickel-based superalloy Alloy 247LC by laser powder bed fusion (L-PBF) is challenging, primarily owing to the alloy’s susceptibility to cracks. Apart from the cracks, voids created during the L-PBF process should also be minimized to produce dense parts. In this study, samples of Alloy 247LC were manufactured by L-PBF, several of which could be produced with voids and crack density close to zero. A statistical design of experiments was used to evaluate the influence of the process parameters, namely laser power, scanning speed, and hatch distance (inherent to the volumetric energy density) on void formation, crack density, and microhardness of the samples. The window of process parameters, in which minimum voids and/or cracks were present, was predicted. It was shown that the void content increased steeply at a volumetric energy density threshold below 81 J/mm3. The crack density, on the other hand, increased steeply at a volumetric energy density threshold above 163 J/mm3. The microhardness displayed a relatively low value in three samples which displayed the lowest volumetric energy density and highest void content. It was also observed that two samples, which displayed the highest volumetric energy density and crack density, demonstrated a relatively high microhardness; which could be a vital evidence in future investigations to determine the fundamental mechanism of cracking. The laser power was concluded to be the strongest and statistically most significant process parameter that influenced void formation and microhardness. The interaction of laser power and hatch distance was the strongest and most significant factor that influenced the crack density.

Beschreibung: Laser-based technologies are extensively used for polymer surface patterning and/or texturing. Different micro- and nanostructures can be obtained thanks to a wide range of laser types and beam parameters. Cell behavior on various types of materials is an extensively investigated phenomenon in biomedical applications. Polymer topography such as height, diameter, and spacing of the patterning will cause different cell responses, which can also vary depending on the utilized cell types. Structurization can highly improve the biological performance of the material without any need for chemical modification. The aim of the study was to evaluate the effect of CO2 laser irradiation of poly(L-lactide) (PLLA) thin films on the surface microhardness, roughness, wettability, and cytocompatibility. The conducted testing showed that CO2 laser texturing of PLLA provides the ability to adjust the structural and physical properties of the PLLA surface to the requirements of the cells despite significant changes in the mechanical properties of the laser-treated surface polymer.

Beschreibung: The convenient model Arabidopsis thaliana has allowed tremendous advances in plant genetics and physiology, in spite of only being a weed. It has also unveiled the main molecular networks governing, among others, abiotic stress responses. Through the use of the latest genomic tools, Arabidopsis research is nowadays being translated to agronomically interesting crop models such as tomato, but at a lagging pace. Knowledge transfer has been hindered by invariable differences in plant architecture and behaviour, as well as the divergent direct objectives of research in Arabidopsis vs. crops compromise transferability. In this sense, phenotype translation is still a very complex matter. Here, we point out the challenges of “translational phenotyping” in the case study of drought stress phenotyping in Arabidopsis and tomato. After briefly defining and describing drought stress and survival strategies, we compare drought stress protocols and phenotyping techniques most commonly used in the two species, and discuss their potential to gain insights, which are truly transferable between species. This review is intended to be a starting point for discussion about translational phenotyping approaches among plant scientists, and provides a useful compendium of methods and techniques used in modern phenotyping for this specific plant pair as a case study.

Beschreibung: Information and communication technologies transform modern education into a more available learning matrix. One of the unexplored aspects of open education is the constant communicative interaction within the student group by using social media. The aim of the study was to determine principal functions of student-led communication in the educational process, the method for assessing its strong points and the disadvantages disrupting traditional learning. For the primary study of the phenomenon, we used methods that made it possible to propose approaches to further analysis. Netnography is the main research method defining the essence and characteristics of the student-led peer-communication. In our research, we applied data visualization, analytical and quantitative methods and developed a set of quantitative indicators that can be used to assess various aspects of student communication in chats. The elaborated visual model can serve as a simple tool for diagnosing group communication processes. We revealed that online group chats perform a support function in learning. They provide constant informational resource on educational and organizational issues and create emotional comfort. Identified features serve to define shortcomings (e.g., lack of students’ readiness to freely exchange answers to assignments) and significant factors (e.g., underutilized opportunities for self-organization) that exist in the modern system of higher education.

Beschreibung: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an RNA virus, is responsible for the coronavirus disease 2019 (COVID-19) pandemic of 2020. Experimental evidence suggests that microRNA can mediate an intracellular defence mechanism against some RNA viruses. The purpose of this study was to identify microRNA with predicted binding sites in the SARS-CoV-2 genome, compare these to their microRNA expression profiles in lung epithelial tissue and make inference towards possible roles for microRNA in mitigating coronavirus infection. We hypothesize that high expression of specific coronavirus-targeting microRNA in lung epithelia may protect against infection and viral propagation, conversely, low expression may confer susceptibility to infection. We have identified 128 human microRNA with potential to target the SARS-CoV-2 genome, most of which have very low expression in lung epithelia. Six of these 128 microRNA are differentially expressed upon in vitro infection of SARS-CoV-2. Additionally, 28 microRNA also target the SARS-CoV genome while 23 microRNA target the MERS-CoV genome. We also found that a number of microRNA are commonly identified in two other studies. Further research into identifying bona fide coronavirus targeting microRNA will be useful in understanding the importance of microRNA as a cellular defence mechanism against pathogenic coronavirus infections.

Beschreibung: The VCAN/versican gene encodes an important component of the extracellular matrix, the chondroitin sulfate proteoglycan 2 (CSPG2/versican). Heterozygous variants targeting exon 8 of VCAN have been shown to cause Wagner disease, a rare autosomal dominant non-syndromic vitreoretinopathy that induces retinal detachment, cataracts and permanent visual loss. In this study, we report on six patients from three unrelated families with Wagner disease in whom we identified three novel copy number variations of VCAN. Quantitative real-time polymerase chain reaction analysis identified deletions, including one exon–intron boundary of exon 8 or both exons 8 and 9, causing the haploinsufficiency of VCAN mRNAs.

Beschreibung: Highly controlled biomineralization of calcium carbonate is via non-classical mesocrystallization of amorphous precursors. In the present study, a simple in vitro assay was developed to mimic the biological process, which involved stabilized amorphous calcium carbonate and a single crystal substrate of calcite. The microcoating layer formed on the calcite substrate displayed mesocrystalline characteristics, and the layers near the substrate were strongly influenced by the epitaxy to the substrate. This behavior was preserved even when the morphology of the coating layer was modified with poly(acrylic acid), a model anionic macromolecule. Interestingly, the extent of the epitaxy increased substantially with poly(ethylene imine), which barely affected the crystal morphology. The in vitro assay in the present study will be useful in the investigations of the biomineralization and bioinspired crystallization of calcium carbonate in general.

Beschreibung: Streptococcus salivarius is a significant contributor to the human oral, pharyngeal and gut microbiomes that contribute to the maintenance of health. The high genomic diversity observed in this species is mainly caused by horizontal gene transfer. This work aimed to evaluate the contribution of integrative and conjugative elements (ICEs) and integrative and mobilizable elements (IMEs) in S. salivarius genome diversity. For this purpose, we performed an in-depth analysis of 75 genomes of S. salivarius and searched for signature genes of conjugative and mobilizable elements. This analysis led to the retrieval of 69 ICEs, 165 IMEs and many decayed elements showing their high prevalence in S. salivarius genomes. The identification of almost all ICE and IME boundaries allowed the identification of the genes in which these elements are inserted. Furthermore, the exhaustive analysis of the adaptation genes carried by these elements showed that they encode numerous functions such as resistance to stress, to antibiotics or to toxic compounds, and numerous enzymes involved in diverse cellular metabolic pathways. These data support the idea that not only ICEs but also IMEs and decayed elements play an important role in S. salivarius adaptation to the environment.

Beschreibung: Stearoyl-CoA desaturase (SCD) is known to be an important rate-limiting enzyme in the production of monounsaturated fatty acids (MUFAs). However, the role of this enzyme in goose follicular development is poorly understood. To investigate the metabolic mechanism of SCD during goose follicular development, we observed its expression patterns in vivo and in vitro using quantitative reverse-transcription (qRT)-PCR. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to determine a cellular model of SCD function in granulosa cells (GCs) via SCD overexpression and knockdown. qRT-PCR analysis showed that SCD was abundantly expressed in the GC layer, and was upregulated in preovulatory follicles. Peak expression was found in F1 and prehierarchal follicles with diameters of 4–6 mm and 8–10 mm, respectively. We further found that mRNA expression and corresponding enzyme activity occur in a time-dependent oscillation pattern in vitro, beginning on the first day of GC culture. By LC-MS/MS, we identified numerous changes in metabolite activation and developed an overview of multiple metabolic pathways, 10 of which were associated with lipid metabolism and enriched in both the overexpressed and knockdown groups. Finally, we confirmed cholesterol and pantothenol or pantothenate as potential metabolite biomarkers to study SCD-related lipid metabolism in goose GCs.

Beschreibung: Ti6Al4V alloy is still attracting great interest because of its application as an implant material for hard tissue repair. This research aims to produce and investigate in-situ chitosan/hydroxyapatite (CS/HA) nanocomposite coatings based on different amounts of HA (10, 50 and 60 wt.%) on alkali-treated Ti6Al4V substrate through the sol-gel process to enhance in vitro bioactivity. The influence of different contents of HA on the morphology, contact angle, roughness, adhesion strength, and in vitro bioactivity of the CS/HA coatings was studied. Results confirmed that, with increasing the HA content, the surface morphology of crack-free CS/HA coatings changed for nucleation modification and HA nanocrystals growth, and consequently, the surface roughness of the coatings increased. Furthermore, the bioactivity of the CS/HA nanocomposite coatings enhanced bone-like apatite layer formation on the material surface with increasing HA content. Moreover, CS/HA nanocomposite coatings were biocompatible and, in particular, CS/10 wt.% HA composition significantly promoted human mesenchymal stem cells (hMSCs) proliferation. In particular, these results demonstrate that the treatment strategy used during the bioprocess was able to improve in vitro properties enough to meet the clinical performance. Indeed, it is predicted that the dense and crack-free CS/HA nanocomposite coatings suggest good potential application as dental implants.

Beschreibung: This paper presents the studies of the development of a high-performance epoxy coating for steel substrates. To this end, it investigated the synergistic effect of incorporating zinc oxide (ZnO) nanoparticles into nanosilica containing epoxy formulations. The mechanical properties of the epoxy coating formulations were improved by modifying the surfaces of the silica nanoparticles (5 wt.%) with 3-glycidoxypropyl trimethoxysilane, which ensured their dispersal through the material. Next, the ZnO nanoparticles (1, 2, or 3 wt.%) were incorporated to improve the corrosion performance of the formulations. The anticorrosive properties of the coatings were examined by electrochemical impedance spectroscopy (EIS) of coated mild steel specimens immersed in 3.5% NaCl solution over different time intervals (1 h to 30 days). Incorporation of the ZnO nanoparticles and the nanosilica into the coating formulation improved the corrosion resistance of the epoxy coating even after long-term exposure to saline test solutions. Finally, to evaluate how the nanoparticles affected the chemical and morphological properties of the prepared coatings, the coatings were characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD).

Beschreibung: In the present paper, the identification of the material parameters of a masonry lighthouse is discussed. A fully non-invasive method was selected, in which the material properties were determined via numerical model validation applied to the first pair of natural frequencies and their related mode shapes, determined experimentally. The exact structural model was built by means of the finite element method. To obtain experimental data for the inverse analysis, operational modal analysis was applied to the structure. Three methods were considered: peak picking (PP), eigensystem realization algorithm (ERA) and natural excitation technique with ERA (NExT-ERA). The acceleration’s responses to environmental excitations, enhanced in some periods of time by sheet piling hammering or by sudden interruptions like wind stroke, were assumed within the analysis input. Different combinations of the input were considered in the PP and NExT-ERA analysis to find the most reasonable modal forms. A number of time periods of a free-decay character were considered in the ERA technique to finally calculate the averaged modal forms. Finally, the elastic modulus, Poisson’s ratio and material density of brick, sandstone and granite masonry were determined. The obtained values supplement the state of the art database concerning historic building materials. In addition, the numerical model obtained in the analysis may be used in further cases of structural analysis.

Beschreibung: Land-based sources are the key sources of plastic debris, and mismanaged plastic debris can eventually enter the ocean via marine beaches. In this study, the spatial distribution and amount of plastic debris in the land-based source input zone of First Long Beach (FLB), China, which is a major tourist attraction, were first investigated. By using field investigation, sand samples were collected from two sections on FLB adjacent to land-based sources in December 2019, and the plastic debris in the sand samples was quantified and characterized in the laboratory. The amount of plastic debris ranged from 2 to 82 particles/m2 on this marine sand beach. There was a significant difference in plastic debris amount between the transects along the land-based source input zone (p 〈 0.05) due to the impacts of wind, ocean currents, and waves. The most abundant size of plastics was 0.5–2.5 cm (44.4%). Moreover, the most common color was white (60.9%). The most abundant shape of plastic debris fell into the fragment category (76.2%). The plastic debris amounts were significantly correlated with multiple sizes. Our results show that land-based wastewater discharge is a large plastic debris source on FLB under coastal water tide variation. Reduction strategies should be carried out by tracing the various land-based sources of plastic debris.

Beschreibung: Water mites represent the most diverse and abundant group of Arachnida in freshwater ecosystems, with about 6000 species described; however, it is estimated that this number represents only 30% of the total expected species. Despite having strong biotic interactions with their community and having the potential to be exceptional bioindicators, they are frequently excluded from studies of water quality or ecology, due to actual and perceived difficulties of taxonomic identification in this group. The objective of this study is to use the variations in the sequences of the mitochondrial cytochrome oxidase subunit I (COI), also known as the DNA barcodes region, as a tool to assess the diversity of water mites at 24 sites in the Yucatan Peninsula of Mexico. We found 77 genetic groups or putative species corresponding to 18 genera: Arrenurus, Atractides, Centrolimnesia, Eylais, Geayia, Hydrodroma, Hydryphantes, Hygrobates, Koenikea, Krendowskia, Limnesia, Limnochares, Mamersellides, Mideopsis, Neumania, Piona, Torrenticola, and Unionicola. This was significant, since there are only 35 species described for this region. Furthermore, this molecular information has allowed us to infer that there are characteristic assemblies per site. These data will facilitate the incorporation of water mites in different studies while the curatorial work continues to assign a Linnaean name.

Beschreibung: Some special conditions are important for chemical syntheses, such as high temperature and the medium used; unfortunately, uncontrollable influences are introduced during the process, resulting in unexpectedly low repeatability. Herein, we report a facile, environmentally friendly, stable, and repeatable methodology for synthesizing silver nanoplates (SNPs) at 0 °C that overcomes these issues and dramatically increases the yield. This method mainly employs sodium dodecyl sulfate (SDS) and sodium alginate (SA) as the surface stabilizer and assistant, respectively. Consequently, we produced hexagonal nanoplates and tailed nanoplates, and the characterization showed that SA dominates the clear and regular profiles of nanoplates at 0 °C. The tailed nanoplates, over time, showed the growth of heads and the dissolving of tails, and inclined to the nanoplates without tails. The synthesis method for SNPs used in this study—0 °C without media—showed high repeatability. We confirmed that these special conditions are not required for the synthesis of silver nanostructures (SNSs). Furthermore, we constructed a new method for preparing noble metal nanostructures and proved the possibility of preparing metal nanostructures at 0 °C.

Beschreibung: Urban areas may contain a wide range of potential habitats and environmental gradients and, given the many benefits to human health and well-being, there is a growing interest in maximizing their biodiversity potential. However, the ecological patterns and processes in urban areas are poorly understood. Using a widely applicable ecological survey method, we sampled epiphytic lichen communities, important bioindicators of atmospheric pollution, on host Quercus trees in urban parks of London, UK, to test if common patterns relating to lichen diversity are mirrored in urban green spaces. We found lichen diversity to be dependent on host species identity, and negatively related to local tree crowding. In addition, we found a strong negative effect of tree size on lichen diversity, leaving large trees as unexploited niches. A novel network analysis revealed the presence of only pioneer communities, showing the lichen communities are being held in successional stasis, likely due to the heritage effects of SO2 emissions and current nitrogen pollution and particulate emissions. Our study highlights that jointly assessing species richness, community structure and the successional stage can be key to understanding diversity patterns in urban ecosystems. Subsequently, this may help best determine the optimum conditions that will facilitate biodiversity increase within cities.

Beschreibung: This research group has recently used the new technology Distributed Acoustic Sensing (DAS) for the monitoring and the measurement of airplane flutter. To the authors’ knowledge, this is the first such use for this new technology. Traditionally, the measurement of airplane flutter requires the mounting of a very large number of sensors on the wing being monitored, and extensive wiring must be connected to all these sensors. The new system and technology introduced in this paper dramatically reduces the hardware requirements in such an application: all the traditional sensors and wiring are replaced with one fiber optic cable with a diameter of 2 mm. An electro-optical system with the size of a desktop PC monitors simultaneously one or more of such fiber optic cables and detects/characterizes any mechanical disturbances on the cables. Theoretical and experimental results are given.

Beschreibung: The objective of our research is to improve the properties of calcium-sulphate hemihydrate parts printed by binder jetting. In this paper, we show the thermal treatment results when using temperature time ramps on binder-jetted ceramic parts without infiltrating. The results show that the mechanical properties of printed parts are improved substantially. Two different thermal cycles were investigated for their effect on the dehydration process of CaSO4·½H2O using infrared analysis. The thermal-treated samples were compared with respect to porosity, surface roughness, compression strength and dimensional and weight variation. The proposed thermal treatment significantly improves the compression strength in a short time, guaranteeing dimensional stability while providing a good surface. This improvement in mechanical properties offers a great chance for using binder-jetted parts as functional components, for example, in the casting field or the medical sector (scaffolds).

Beschreibung: Marek’s disease (MD) represents a significant global economic and animal welfare issue. Marek’s disease virus (MDV) is a highly contagious oncogenic and highly immune-suppressive α-herpes virus, which infects chickens, causing neurological effects and tumour formation. Though partially controlled by vaccination, MD continues to have a profound impact on animal health and on the poultry industry. Genetic selection provides an alternative and complementary method to vaccination. However, even after years of study, the genetic mechanisms underlying resistance to MDV remain poorly understood. The Major Histocompatability Complex (MHC) is known to play a role in disease resistance, along with a handful of other non-MHC genes. In this study, one of the largest to date, we used a multi-facetted approach to identify quantitative trait locus regions (QTLR) influencing resistance to MDV, including an F6 population from a full-sib advanced intercross line (FSIL) between two elite commercial layer lines differing in resistance to MDV, RNA-seq information from virus challenged chicks, and genome wide association study (GWAS) from multiple commercial lines. Candidate genomic elements residing in the QTLR were further tested for association with offspring mortality in the face of MDV challenge in eight pure lines of elite egg-layer birds. Thirty-eight QTLR were found on 19 chicken chromosomes. Candidate genes, microRNAs, long non-coding RNAs and potentially functional mutations were identified in these regions. Association tests were carried out in 26 of the QTLR, using eight pure lines of elite egg-layer birds. Numerous candidate genomic elements were strongly associated with MD resistance. Genomic regions significantly associated with resistance to MDV were mapped and candidate genes identified. Various QTLR elements were shown to have a strong genetic association with resistance. These results provide a large number of significant targets for mitigating the effects of MDV infection on both poultry health and the economy, whether by means of selective breeding, improved vaccine design, or gene-editing technologies.

Beschreibung: The human microbiome is comprised of the microbes that live on and within an individual, as well as immediately surrounding them. Microbial profiling may have forensic utility in the identification or association of individuals with criminal activities, using microbial signatures derived from a personal microbiome. This review highlights some important aspects of recent studies, many of which have revealed issues involving the effect of contamination of microbial samples from both technical and environmental sources and their impacts on microbiome research and the potential forensic applications of microbial profiling. It is imperative that these challenges be discussed and evaluated within a forensic context to better understand the future directions and potential applications of microbial profiling for human identification. It is necessary that the limitations identified be resolved prior to the adoption of microbial profiling, or, at a minimum, acknowledged by those applying this new approach.

Beschreibung: As the use of continuous fiber polymer matrix composites expands into new fields, there is a growing need for more sustainable manufacturing processes. An integrated computational material design framework has been developed, which enables the design of tailored manufacturing systems for polymer matrix composite materials as a sustainable alternative to achieving high-quality components in high-rate production. Trapped rubber processing achieves high pressures during polymer matrix composite processing, utilizing the thermally induced volume change of a nearly incompressible material inside a closed cavity mold. In this interdisciplinary study, the structural analysis, material science and manufacturing engineering perspectives are all combined to determine the mold mechanics, and the manufacturing process in a cohesive and iterative design loop. This study performs the coupled thermo-mechanical analysis required to simulate the transients involved in composite manufacturing and the results are compared with a previously developed test method. The internal surface pressure and temperatures are computed, compared with the experimental results, and the resulting design process is simulated. Overall, this approach maintains high-quality consolidation during curing while allowing for the possibility for custom distributions of pressures and temperatures. This can lead to more sustainable manufacturing by reducing energy consumption and improving throughput.

Beschreibung: The aim of this study was to investigate the impact of distributed propulsion systems used on inland and coastal navigation in shallow water. Five layouts were assessed by computational fluid dynamics (CFD) simulation. The hull/propulsion layout cases have been analyzed for discrete flow speed values in the range 0–6 m/s. All cases have been examined under restricted draft conditions in shallow water with a minimum of 0.3 m under keel clearance (UKC) and under unrestricted draft conditions in deep water. The results show that distributed propulsion consisting of 6 or 8 (in some cases, even more) units produces noticeable higher thrust effects in shallow water than the traditional layout. Under restricted conditions, the thrust increase between two distributed layouts with different numbers of propulsors is higher, in contrast to deep water, where differences in performance are not so significant.

Beschreibung: Xenogenic collagen-based matrices represent an alternative to subepithelial palatal connective tissue autografts in periodontal and peri-implant soft tissue reconstructions. In the present study, we aimed to investigate the migratory, adhesive, proliferative, and wound-healing potential of primary human oral fibroblasts (hOF) and periodontal ligament cells (hPDL) in response to four commercially available collagen matrices. Non-crosslinked collagen matrix (NCM), crosslinked collagen matrix (CCM), dried acellular dermal matrix (DADM), and hydrated acellular dermal matrix (HADM) were all able to significantly enhance the ability of hPDL and hOF cells to directionally migrate toward the matrices as well as to efficiently repopulate an artificially generated wound gap covered by the matrices. Compared to NCM and DADM, CCM and HADM triggered stronger migratory response. Cells grown on CCM and HADM demonstrated significantly higher proliferative rates compared to cells grown on cell culture plastic, NCM, or DADM. The pro-proliferative effect of the matrices was supported by expression analysis of proliferative markers regulating cell cycle progression. Upregulated expression of genes encoding the adhesive molecules fibronectin, vinculin, CD44 antigen, and the intracellular adhesive molecule-1 was detected in hPDL and hOF cells cultured on each of the four matrices. This may be considered as a prerequisite for good adhesive properties of the four scaffolds ensuring proper cell–matrix and cell–cell interactions. Upregulated expression of genes encoding TGF-β1 and EGF growth factors as well as MMPs in cells grown on each of the four matrices provided support for their pro-proliferative and pro-migratory abilities. The expression of genes encoding the angiogenic factors FGF-2 and VEGF-A was dramatically increased in cells grown on DADM and HADM only, suggesting a good basis for accelerated vascularization of the latter. Altogether, our results support favorable influence of the investigated collagen matrices on the recruitment, attachment, and growth of cell types implicated in oral soft tissue regeneration. Among the four matrices, HADM has consistently exhibited stronger positive effects on the oral cellular behavior. Our data provide solid basis for future investigations on the clinical application of the collagen-based matrices in surgical periodontal therapy.

Beschreibung: Due to the growing success of neural machine translation (NMT), many have started to question its applicability within the field of literary translation. In order to grasp the possibilities of NMT, we studied the output of the neural machine system of Google Translate (GNMT) and DeepL when applied to four classic novels translated from English into Dutch. The quality of the NMT systems is discussed by focusing on manual annotations, and we also employed various metrics in order to get an insight into lexical richness, local cohesion, syntactic, and stylistic difference. Firstly, we discovered that a large proportion of the translated sentences contained errors. We also observed a lower level of lexical richness and local cohesion in the NMTs compared to the human translations. In addition, NMTs are more likely to follow the syntactic structure of a source sentence, whereas human translations can differ. Lastly, the human translations deviate from the machine translations in style.

Beschreibung: Concrete wastewater infrastructures are important to modern society but are susceptible to sulfuric acid attack when exposed to an aggressive environment. Fibre-reinforced mortar has been adopted as a promising coating and lining material for degraded reinforced concrete structures due to its unique crack control and excellent anti-corrosion ability. This paper aims to evaluate the performance of polyethylene (PE) fibre-reinforced calcium aluminate cement (CAC)–ground granulated blast furnace slag (GGBFS) blended strain-hardening mortar after sulfuric acid immersion, which represented the aggressive sewer environment. Specimens were exposed to 3% sulfuric acid solution for up to 112 days. Visual, physical and mechanical performance such as water absorption ability, sorptivity, compressive and direct tensile strength were evaluated before and after sulfuric acid attack. In addition, micro-structure changes to the samples after sulfuric acid attack were also assessed by X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM) to further understand the deterioration mechanism. The results show that overall fibre-reinforced calcium aluminate cement (CAC)-based samples performed significantly better than fibre-reinforced ordinary Portland cement (OPC)-based samples as well as mortar samples in sulfuric acid solution in regard to visual observations, penetration depth, direct tensile strength and compressive reduction. Gypsum generation in the cementitious matrix of both CAC and OPC-based systems was the main reason behind the deterioration mechanism after acid attack exposure. Moreover, laboratory sulfuric acid testing has been proven for successfully screening the cementitious material against an acidic environment. This method can be considered to design the service life of concrete wastewater pipes.

Beschreibung: The emergence and outbreak of the novel coronavirus (COVID-19) had a devasting effect on global health, the economy, and individuals’ daily lives. Timely diagnosis of COVID-19 is a crucial task, as it reduces the risk of pandemic spread, and early treatment will save patients’ life. Due to the time-consuming, complex nature, and high false-negative rate of the gold-standard RT-PCR test used for the diagnosis of COVID-19, the need for an additional diagnosis method has increased. Studies have proved the significance of X-ray images for the diagnosis of COVID-19. The dissemination of deep-learning techniques on X-ray images can automate the diagnosis process and serve as an assistive tool for radiologists. In this study, we used four deep-learning models—DenseNet121, ResNet50, VGG16, and VGG19—using the transfer-learning concept for the diagnosis of X-ray images as COVID-19 or normal. In the proposed study, VGG16 and VGG19 outperformed the other two deep-learning models. The study achieved an overall classification accuracy of 99.3%.

Beschreibung: Incidental findings on newborn screening (NBS) are results that are not the target of screening within a given NBS program, but rather are found as a result of the screening and resulting diagnostic workup for that target. These findings may not have an immediate clinical impact on the newborn, but are sometimes an additional benefit of NBS programs and may be considered secondary targets of NBS programs. This work describes four case reports that had incidental findings on the NBS, which eventually led to the diagnosis of another metabolic disease instead of the one that was initially suspected. The first case was a new defect in the cationic amino acid transporter-2 (CAT-2), which was oriented as an arginase-1 deficiency in the newborn. The second case was a maternal glutaric aciduria type 1 (GA-1) that mimicked a carnitine transporter deficiency in the newborn. The third report was a case of lysinuric protein intolerance (LPI), which appeared as high levels of citrulline on the NBS. The fourth case was a mother with homocystinuria that was diagnosed during the biochemical study of vitamin B12 status. All cases provide new or interesting data that will help guide differential diagnosis in the future.

Beschreibung: In domesticated strains of the Nile tilapia, phenotypic sex has been linked to genetic variants on linkage groups 1, 20 and 23. This diversity of sex-loci might reflect a naturally polymorphic sex determination system in Nile tilapia, or it might be an artefact arising from the process of domestication. Here, we searched for sex-determiners in wild populations from Kpandu, Lake Volta (Ghana-West Africa), and from Lake Koka (Ethiopia-East Africa) that have not been subjected to any genetic manipulation. We analysed lab-reared families using double-digest Restriction Associated DNA sequencing (ddRAD) and analysed wild-caught males and females with pooled whole-genome sequencing (WGS). Strong sex-linked signals were found on LG23 in both populations, and sex-linked signals with LG3 were observed in Kpandu samples. WGS uncovered blocks of high sequence coverage, suggesting the presence of B chromosomes. We confirmed the existence of a tandem amh duplication in LG23 in both populations and determined its breakpoints between the oaz1 and dot1l genes. We found two common deletions of ~5 kb in males and confirmed the presence of both amhY and amh∆Y genes. Males from Lake Koka lack both the previously reported 234 bp deletion and the 5 bp frameshift-insertion that creates a premature stop codon in amh∆Y.

Beschreibung: The Brazilian Cerrado biome has undergone major changes, with the incorporation of new areas for agricultural production. While this can certainly provide for the worldwide growing need for agricultural products, especially food, care should be taken to prevent possible environmental degradation. Worldwide, mites of the cohort Gamasina constitute the most abundant and diverse group of soil predatory mites, usually considered important in maintaining the ecological balance of natural environments. Little is known about the abundance and diversity of Gamasina in the Cerrado. The objective of the present work was to evaluate the abundance and diversity of Gamasina in soils of natural vegetation and of agroecosystems in Cerrado areas of the northern Brazilian state of Tocantins. This is considered the first step in the determination of possible role of the local predators as biological control agents, and their potential for practical use locally and elsewhere. Soil samples were taken monthly between July 2015 and June 2016. In total, 1373 Gamasina representing 45 species of 24 genera and 9 families were collected. The most abundant Gamasina belonged to Rhodacaridae in areas of the natural vegetation and to Ascidae in the agroecosystems. Abundance and diversity were much higher in the rainy than in the dry season. Rhodacarids and ascids have not been used commercially for pest control, but investigations conducted so far suggest their potential as biological control agents. The confirmation of this possibility and the development of techniques that would allow their maintenance in agricultural areas require subsequent research efforts.

Beschreibung: Incorporating various industrial waste materials into concrete has recently gained attention for sustainable construction. This paper, for the first time, studies the effects of silica stone waste (SSW) powder on concrete. The cement of concrete was replaced with 5, 10, 15, and 20% of the SSW powder. The mechanical properties of concrete, such as compressive and tensile strength, were studied. Furthermore, the microstructure of concrete was studied by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy analysis (EDX), Fourier transformed infrared spectroscopy (FTIR), and X-Ray diffraction (XRD) tests. Compressive and tensile strength of samples with 5% SSW powder was improved up to 18.8% and 10.46%, respectively. As can be observed in the SEM images, a reduced number of pores and higher density in the matrix can explain the better compressive strength of samples with 5% SSW powder.

Beschreibung: This paper presents a study of the Ti-6Al-4V alloy milling under different lubrication conditions, using the minimum quantity lubrication approach. The chosen material is widely used in the industry due to its properties, although they present difficulties in terms of their machinability. A minimum quantity lubrication (MQL) prototype valve was built for this purpose, and machining followed a previously defined experimental design with three lubrication strategies. Speed, feed rate, and the depth of cut were considered as independent variables. As design-dependent variables, cutting forces, torque, and roughness were considered. The desirability optimization function was used in order to obtain the best input data indications, in order to minimize cutting and roughness efforts. Supervised artificial neural networks of the multilayer perceptron type were created and tested, and their responses were compared statistically to the results of the factorial design. It was noted that the variables that most influenced the machining-dependent variables were the feed rate and the depth of cut. A lower roughness value was achieved with MQL only with the use of cutting fluid with graphite. Statistical analysis demonstrated that artificial neural network and the experimental design predict similar results.

Beschreibung: In this work, we demonstrate how the blockchain and the off-chain storage interact via Oracle-based mechanisms, which build an effective connection between a distributed database and real assets. For demonstration purposes, smart contracts were drawn up to deal with two different applications. Due to the characteristics of the blockchain, we may still encounter severe privacy issues, since the data stored on the blockchain are exposed to the public. The proposed scheme provides a general solution for resolving the above-mentioned privacy issue; that is, we try to protect the on-chain privacy of the sensitive data by using homomorphic encryption techniques. Specifically, we constructed a secure comparison protocol that can check the correctness of a logic function directly in the encrypted domain. By using the proposed access control contract and the secure comparison protocol, one can carry out sensitive data-dependent smart contract operations without revealing the data themselves.

Beschreibung: Healthcare facilities are constantly deteriorating due to tight budgets allocated to the upkeep of building assets. This entails the need for improved deterioration modeling of such buildings in order to enforce a predictive maintenance approach that decreases the unexpected occurrence of failures and the corresponding downtime elapsed to repair or replace the faulty asset components. Currently, hospitals utilize subjective deterioration prediction methodologies that mostly rely on age as the sole indicator of degradation to forecast the useful lives of the building components. Thus, this paper aims at formulating a more efficient stochastic deterioration prediction model that integrates the latest observed condition into the forecasting procedure to overcome the subjectivity and uncertainties associated with the currently employed methods. This is achieved by means of developing a hybrid genetic algorithm-based fuzzy Markovian model that simulates the deterioration process given the scarcity of available data demonstrating the condition assessment and evaluation for such critical facilities. A nonhomogeneous transition probability matrix (TPM) based on fuzzy membership functions representing the condition, age and relative deterioration rate of the hospital systems is utilized to address the inherited uncertainties. The TPM is further calibrated by means of a genetic algorithm to circumvent the drawbacks of the expert-based models. A sensitivity analysis was carried out to analyze the possible changes in the output resulting from predefined modifications to the input parameters in order to ensure the robustness of the model. The performance of the deterioration prediction model developed is then validated through a comparison with a state-of-art stochastic model in contrast to real hospital datasets, and the results obtained from the developed model significantly outperformed the long-established Weibull distribution-based deterioration prediction methodology with mean absolute errors of 1.405 and 9.852, respectively. Therefore, the developed model is expected to assist decision-makers in creating more efficient maintenance programs as well as more data-driven capital renewal plans.

Beschreibung: BiFeO3 nanocrystals were applied as the sensing material to isopropanol. The isopropanol sensor based on BiFeO3 nanocrystals shows excellent gas-sensing properties at the optimum working temperature of 240 °C. The sensitivity of as-prepared sensor to 100 ppm isopropanol is 31 and its response and recovery time is as fast as 6 and 17 s. The logarithmic curves of the sensitivity and concentration of BiFeO3 sensors are a very good linear in the low detection range of 2–100 ppm. In addition, the gas sensing mechanism is also discussed. The results suggest that the BiFeO3 nanomaterial can be potentially applied in isopropanol gas detection.

Beschreibung: For the first time, an original compound belonging to the heptazine family has been deposited in the form of thin layers, both by thermal evaporation under vacuum and spin-coating techniques. In both cases, smooth and homogeneous layers have been obtained, and their properties evaluated for eventual applications in the field of organic electronics. The layers have been fully characterized by several concordant techniques, namely UV-visible spectroscopy, steady-state and transient fluorescence in the solid-state, as well as topographic and conductive atomic force microscopy (AFM) used in Kelvin probe force mode (KPFM). Consequently, the afferent energy levels, including Fermi level, have been determined, and show that these new heptazines are promising materials for tailoring the electronic properties of interfaces associated with printed electronic devices. A test experiment showing an improved electron transfer rate from a tris-(8-hydroxyquinoline) aluminum (Alq3) photo-active layer in presence of a heptazine interlayer is finally presented.

Beschreibung: A possible approach for providing new properties for textiles is the insertion of natural ingredients into the textile product during the process of its manufacture. Myrrh has long been used in medicine as an antibacterial and antifungal material. Polylactide (PLA) is a thermoplastic synthetic biopolymer obtained from renewable resources—and due its biodegradability, is also widely used in medicine. In this study, films and multifilament yarns from modified biodegradable PLA granules with ethanolic and aqueous myrrh extracts were developed and characterized. Optical microscopy was used to determine the surface morphology of PLA/myrrh multifilament yarns. Tensile tests, ultraviolet-visible (UV-vis), differential scanning calorimetry (DSC) were applied to determine, consequently, mechanical, optical properties and degree of crystallinity of PLA/myrrh films and multifilament yarns. The chemical composition of PLA/myrrh multifilament yarns was estimated by Fourier-transform infrared (FTIR) spectroscopy method. The results showed that it is possible to form PLA melt-spun multifilament yarns with myrrh extract. The type of myrrh extract (ethanolic or aqueous) has a significant influence on the mechanical and optical properties of the PLA films and melt-spun yarns. The mechanical properties of PLA films and melt-spun multifilament yarns formed from PLA granules with aqueous myrrh extract decreased 19% and 21% more than PLA with ethanolic extract, respectively. Analysis of UV-vis spectra showed that, due to the yellow hue, the reflectance of PLA films and melt-spun PLA multifilament yarns modified with myrrh extracts decreased exponentially. The DSC test showed that multifilament yarns from PLA modified with aqueous extract had the highest degree of crystallization.

Beschreibung: This paper primarily assesses the scale of adverse changes to the compressive strength of different types of silicates due to the influence of moisture. The study covers three groups of silicate units of different strength classes—15, 20 and 25—obtained from three different manufacturers. It was demonstrated that in all studied groups, moisture significantly decreased the compressive strength by about 30–40%. In addition, microstructural studies were conducted to analyze the relationship between the specific porosity structure of each group of silicate bricks and their compressive strength. On the basis of SEM (Scanning Electron Microscopy) and EDS (Electron Dispersive Spectroscopy) analysis, the elemental composition of individual silicates was determined and the contact zone between the aggregate and the binder was determined, which largely influenced the obtained compressive strength of each silicates. Next, the study referred to the utility of the normative procedure used to determine the strength class of samples with different geometries and at different moisture concentrations. The results of the calculations showed the high accuracy of the normative-based assessment of strength class, regardless of the manufacturer and the moisture values during examination.

Beschreibung: Additive manufacturing (AM) is the term for a number of processes for joining materials to build physical components from a digital 3D model. AM has multiple advantages over other construction techniques, such as freeform, customization, and waste reduction. However, AM components have been evaluated by destructive and non-destructive testing and have shown mechanical issues, such as reduced resistance, anisotropy and voids. The build direction affects the mechanical properties of the built part, including voids of different characteristics. The aim of this work is an extended analysis of void shape by means of X-ray computed tomography (CT) applied to fused deposition modeling (FDM) samples. Furthermore, a relation between the tensile mechanical properties and digital void measurements is established. The results of this work demonstrate that void characteristics such as quantity, size, sphericity and compactness show no obvious variations between the samples. However, the angle between the main void axis and the mechanical load axis α shows a relation for FDM components: when its mean value μ(α) is around 80 (degrees) the yield strength and Young’s modulus are reduced. These results lead to the formulation of a novel criterion that predicts the mechanical behavior of AM components.

Beschreibung: The objective of this work is to study the geometric properties of surface topographies of hot-work tool steel created by electric discharge machining (EDM) using motif and multiscale analysis. The richness of these analyses is tested through calculating the strengths of the correlations between discharge energies and resulting surface characterization parameters, focusing on the most representative surface features—craters, and how they change with scale. Surfaces were created by EDM using estimated energies from 150 to 9468 µJ and measured by focus variation microscope. The measured topographies consist of overlapping microcraters, of which the geometry was characterized using three different analysis: conventional with ISO parameters, and motif and multiscale curvature tensor analysis. Motif analysis uses watershed segmentation which allows extraction and geometrically characterization of each crater. Curvature tensor analysis focuses on the characterization of principal curvatures and their function and their evolution with scale. Strong correlations (R2 〉 0.9) were observed between craters height, diameter, area and curvature using linear and logarithmic regressions. Conventional areal parameter related to heights dispersion were found to correlate stronger using logarithmic regression. Geometric characterization of process-specific topographic formations is considered to be a natural and intuitive way of analyzing the complexity of studied surfaces. The presented approach allows extraction of information directly relating to the shape and size of topographic features of interest. In the tested conditions, the surface finish is mostly affected and potentially controlled by discharge energy at larger scales which is associated with sizes of fabricated craters.

Beschreibung: Laser irradiation is a popular method to produce microtextures on metal surfaces. However, the common laser-produced microtextures were hierarchical (multiscale), which may limit their applicability. In this paper, a method of two-step laser irradiation, combining first-step strong ablation and sequentially second-step gentle ablation, was presented to produce micron-rough surface with single-scale microtextures. The effect of laser fluence on the Ti–6Al–4V surface morphology and wettability were investigated in detail. The morphology results revealed that the microtextures produced using this method gradually evolved from multiscale to single-scale meanwhile from microprotrusions to microholes with increasing the second-step laser fluence from 0.0 to 2.4 J/cm2. The wettability and EDS/XPS results indicated that attributing to the rich TiO2 content and micron roughness produced by laser irradiation, all the two-step laser-irradiated surfaces exhibited superhydrophilicity. In addition, after silanization, all these superhydrophilic surfaces immediately turned to be superhydrophobic with close water contact angles of 155–162°. However, due to the absence of nanotextures, the water-rolling angle on the superhydrophobic surfaces with single-scale microtextures distinctly larger than those with multiscale ones. Finally, using the two-step laser-irradiation method and assisted with silanization, multifunctional superhydrophobic Ti–6Al–4V surfaces were achieved, including self-cleaning, guiding of the water-rolling direction and anisotropic water-rolling angles (like the rice-leaf), etc.

Beschreibung: The ultrasonic modulation technique, developed by inspecting the nonlinearity from the interactions of crack surfaces, has been considered very effective in detecting fatigue cracks in the early stage of the crack development due to its high sensitivity. The wave modulation is the frequency shift of a wave passing through a crack and does not occur in intact specimens. Various parameters affect the modulation of the wave, but quantitative analysis for each variable has not been comprehensively conducted due to the complicated interaction of irregular crack surfaces. In this study, specimens with a constant crack width are manufactured, and the effects of various excitation parameters on modulated wave generation are analyzed. Based on the analysis, an effective crack detection algorithm is proposed and verified by applying the algorithm to fatigue cracks. For the quantitative analysis, tests are repeatedly conducted by varying parameters. As a result, the excitation intensity shows a strong linear relationship with the amount of modulated waves, and the increase of modulated wave is expected as crack length increases. However, the change in the dynamic characteristics of the specimen with the crack length is more dominant in the results. The excitation frequency is the most dominant variable to generate the modulated waves, but a direct correlation is not observed as it is difficult to measure the interaction of crack surfaces. A numerical analysis technique is developed to accurately simulate the movement and interaction of the crack surface. The crack detection algorithm, improved by using the observations from the quantitative analyses, can distinguish the occurrence of modulated waves from the ambient noises, and the state of the specimens is determined by using two nonlinear indexes.