Publication Date:
2018
Description:
〈p〉Publication date: January 2019〈/p〉
〈p〉〈b〉Source:〈/b〉 Fish & Shellfish Immunology, Volume 84〈/p〉
〈p〉Author(s): Yinnan Mu, Shimin Zhou, Ning Ding, Jingqun Ao, Xinhua Chen〈/p〉
〈div xml:lang="en"〉
〈h5〉Abstract〈/h5〉
〈div〉〈p〉Chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. Currently, five subgroups of fish specific CXC chemokines, named CXCL_F1-CXCL_F5, have been identified in teleost fish. However, understanding of the functions of these fish specific CXC chemokines is still limited. Here, a new member of fish specific CXC chemokines, 〈em〉Lc〈/em〉CXCL_F6, was cloned from large yellow croaker 〈em〉Larimichthys crocea〈/em〉. Its open reading frame (ORF) is 369 nucleotides long, encoding a peptide of 122 amino acids (aa). The deduced 〈em〉Lc〈/em〉CXCL_F6 protein contains a 19-aa signal peptide and a 103-aa mature polypeptide, which has four conserved cysteine residues (C〈sup〉28〈/sup〉, C〈sup〉30〈/sup〉, C〈sup〉56〈/sup〉, and C〈sup〉72〈/sup〉), as found in other known CXC chemokines. Phylogenetic analysis showed 〈em〉Lc〈/em〉CXCL_F6 formed a separate clade with sequences from other fish species, tentatively named CXCL_F6, distinct from the clades formed by fish CXCL_F1-5 and mammalian CXC chemokines. The 〈em〉Lc〈/em〉CXCL_F6 transcripts were constitutively expressed in all examined tissues and significantly up-regulated in the spleen and head kidney tissues by poly (I:C) and 〈em〉Vibrio alginolyticus〈/em〉. Its transcripts were also detected in primary head kidney leukocytes (HKLs), peripheral blood leucocytes (PBLs), and large yellow croaker head kidney (LYCK) cell line, and significantly up-regulated by poly(I:C), lipopolysaccharide (LPS), and peptidoglycan (PGN) in HKLs. Recombinant 〈em〉Lc〈/em〉CXCL_F6 protein (r〈em〉Lc〈/em〉CXCL_F6) could not only chemotactically attract monocytes/macrophages and lymphocytes from PBLs, but also enhance NO release and expression of proinflammatory cytokines (TNF-α, IL-1β, and CXCL8) in monocytes/macrophages. These results indicate that 〈em〉Lc〈/em〉CXCL_F6 plays a role in mediating the inflammatory response.〈/p〉〈/div〉
〈/div〉
Print ISSN:
1050-4648
Electronic ISSN:
1095-9947
Topics:
Biology
,
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Medicine
Permalink