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  • J strain  (3)
  • Arctic Ocean
  • Elsevier  (4)
  • American Chemical Society
  • Blackwell Publishing Ltd
  • 2015-2019  (4)
  • 1970-1974
  • 2019  (1)
  • 2017  (3)
  • 1
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 188–193, doi:10.1016/j.ydbio.2016.03.006.
    Description: Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated that TALENs are able to induce mutations in F0 Xenopus embryos, but none has demonstrated germline transmission of such mutations in X. laevis. In this report we used the oocyte host-transfer method to generate mutations in both tyrosinase homeologs and found highly-penetrant germline mutations; in contrast, embryonic injections yielded few germline mutations. We also compared the distribution of mutations in several F0 somatic tissues and germ cells and found that the majority of mutations in each tissue were different. These results establish that X. laevis J strain animals are very useful for generating germline mutations and that the oocyte host-transfer method is an efficient technique for generating mutations in both homeologs.
    Description: This work was supported by grants from the NIH (OD010997 and HD084409).
    Keywords: Xenopus laevis ; TALENs ; J strain ; Tyrosinase ; Oocyte host-transfer ; Genome editing
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 2
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 442–448, doi:10.1016/j.ydbio.2016.05.028.
    Description: Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well as lack of a well tested alternative have resulted in frustrating experimental delays in laboratories that predominantly use Xenopus in their research. Mammalian Luteinizing Hormones (LH) share structural similarity, functional equivalency, and bind the same receptor as hCG; this suggests that LH may serve as a good alternative to hCG for promoting ovulation in Xenopus. LH has been found to induce maturation of Xenopus oocytes in vitro, but whether it can be used to induce ovulation in vivo has not been examined. Here we compared the ability of four mammalian LH proteins, bovine (bLH), human (hLH), ovine (oLH), porcine (pLH), to induce ovulation in Xenopus when injected into the dorsal lymph sac of sexually mature females. We find that both ovine and human LH, but not bovine or porcine, are good substitutes for hCG for induction of ovulation in WT and J strain Xenopus laevis and Xenopus tropicalis.
    Description: This work was supported by a grant from the NIHP40OD010997.
    Keywords: Xenopus laevis ; J strain ; Luteinizing Hormone ; Ovulation ; Chorionic gonadotropin
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 3
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Developmental Biology 426 (2017): 325-335, doi:10.1016/j.ydbio.2016.04.009.
    Description: The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, external development, and simple housing requirements, with additional advantages of large embryos, highly conserved developmental processes, and close evolutionary relationship to higher vertebrates. There are two main species of Xenopus used in biomedical research, Xenopus laevis and Xenopus tropicalis; the common perception is that both species are excellent models for embryological and cell biological studies, but only Xenopus tropicalis is useful as a genetic model. The recent completion of the Xenopus laevis genome sequence combined with implementation of genome editing tools, such as TALENs (transcription activator-like effector nucleases) and CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated nucleases), greatly facilitates the use of both Xenopus laevis and Xenopus tropicalis for understanding gene function in development and disease. In this paper, we review recent advances made in Xenopus laevis and Xenopus tropicalis with TALENs and CRISPR-Cas and discuss the various approaches that have been used to generate knockout and knock-in animals in both species. These advances show that both Xenopus species are useful for genetic approaches and in particular counters the notion that Xenopus laevis is not amenable to genetic manipulations.
    Description: This work was supported by the National Institutes of Health (P40 OD010997 to M.E.H., R01 HD084409 to M.E.H., R01 HL112618 to P.T. and F.C., and R01 HL127640 to P.T. and F.C.; and the U.S. Environmental Protection Agency (G11E10367 to D.F.).
    Keywords: CRISPR-Cas ; TALENs ; J strain ; Xenopus laevis ; Xenopus tropicalis ; Knock-in ; Human disease model
    Repository Name: Woods Hole Open Access Server
    Type: Article
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  • 4
    Publication Date: 2022-05-26
    Description: Author Posting. © The Authors, 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Roca-Marti, M., Puigcorbe, V., Friedrich, J., van der Loeff, M. R., Rabe, B., Korhonen, M., Camara-Mor, P., Garcia-Orellana, J., & Masque, P. (2018). Distribution of pb-210 and po-210 in the arctic water column during the 2007 sea-ice minimum: Particle export in the ice-covered basins. Deep-Sea Research Part I-Oceanographic Research Papers, 142, 94-106, doi:10.1016/j.dsr.2018.09.011.
    Description: 210Pb and 210Po are naturally occurring radionuclides that are commonly used as a proxy for particle and carbon export. In this study, the distribution of the 210Po/210Pb pair was investigated in the water column of the Barents, Kara and Laptev Seas and the Nansen, Amundsen and Makarov Basins in order to understand the particle dynamics in the Arctic Ocean during the 2007 sea-ice minimum (August-September). Minimum activities of total 210Pb and 210Po were found in the upper and lower haloclines (approx. 60-130 m), which are partly attributed to particle scavenging over the shelves, boundary current transport and subsequent advection of the water with low 210Pb and 210Po activities into the central Arctic. Widespread and substantial (〉50%) deficits of 210Po with respect to 210Pb were detected from surface waters to 200 m on the shelves, but also in the basins. This was particularly important in the Makarov Basin where, despite very low chlorophyll-a levels, estimates of annual new primary production were three times higher than in the Eurasian Basin. In the Nansen, Amundsen and Makarov 32 Basins, estimates of annual new primary production correlated with the deficits of 210Po in the upper 200 m of the water column, suggesting that in situ production and subsequent export of biogenic material were the mechanisms that controlled the removal of 210Po in the central Arctic. Unlike 210Po, 234Th deficits measured during the same expedition were found to be very small and not significant below 25 m in the basins (Cai et al., 2010), which indicates, given the shorter half-life of 234Th, that particle export fluxes in the central Arctic would have been higher before July-August in 2007 than later in the season.
    Description: We would like to thank the crew of the R/V Polarstern and the scientists on board for their cooperation during the ARK-XXII/2 expedition. We greatly appreciate the hard work of Oliver Lechtenfeld who collected and processed the samples on board. Thanks to Dorothea Bauch for sharing her results on freshwater origin and Adam Ulfsbo for providing insightful comments on the estimates of primary production. This project was partly supported by the Ministerio de Ciencia e Innovación (CTM2011-28452, Spain). We wish to acknowledge the support of the Generalitat de Catalunya to the research group MERS (2017 SGR-1588). This work is contributing to the ICTA ‘Unit of Excellence’ (MinECo, MDM2015-0552). M.R.-M. was supported by a Spanish PhD fellowship (AP2010-2510) and an Australian postdoctoral fellowship (2017 Endeavour Research Fellowship).
    Description: 2019-10-22
    Keywords: Particle export ; Annual new primary production ; Scavenging ; 210Po/210Pb ; Arctic Ocean ; 2007 sea-ice minimum
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
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