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  • Articles  (13,457)
  • Springer  (9,502)
  • Molecular Diversity Preservation International  (2,289)
  • American Chemical Society (ACS)  (1,666)
  • 2010-2014  (13,457)
  • 2014  (13,457)
  • Process Engineering, Biotechnology, Nutrition Technology  (5,587)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (5,517)
  • Electrical Engineering, Measurement and Control Technology  (3,362)
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  • Articles  (13,457)
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  • 2010-2014  (13,457)
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  • 1
    Publication Date: 2014-12-31
    Print ISSN: 0969-0239
    Electronic ISSN: 1572-882X
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 2
    Publication Date: 2014-12-31
    Description: Previous epidemiological study showed that most of the porcine enterotoxin Escherichia coli (ETEC) strains harbor multiple enterotoxins but lack any of the fimbriae or non-fimbrial adhesion genes. Therefore, effective ETEC vaccines need to aim directly at all the enterotoxin antigens. The objective of this study was to evaluate the simultaneous immune effect of two live attenuated E. coli strains expressing LT R192G -STa A13Q and LT R192G -STb fusion immunogen, respectively. The results showed that both local mucosal and systemic immune responses against LT, STa, STb, and F41 were induced in BALB/c mice immunized orally with the recombinant E. coli strains ER - A and ER - B simultaneously. In addition, results of cellular immune responses showed that stimulation index (SI) values of immunized mice were significantly higher than control mice ( P  〈 0.05) and a marked shift toward type-2 helper T lymphocyte (Th 2) immunity. Moreover, the induced antibodies demonstrated neutralizing effects on LT, STa, and STb producing E. coli infection. These data indicated that the use of recombinant E. coli ER - A and ER - B could be a valuable strategy for future polyvalent vaccine development of ETEC.
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 3
    Publication Date: 2014-12-31
    Description: Human activity has a greater influence on plants than on any of the other components of the environment. We used material from cultivated and wild Coptis teeta populations in the Hengduan Mountains to test the null hypothesis that there is no effect of human activity on the biomass allocation of an herbaceous understory species in a forest ecosystem. At the plot level, an isometric relationship existed in most of the significant relationships. However, at the individual level, an allometric relationship existed in most of the log–log relationships. Human activity could significantly affect the slope of the linear relationship of leaf versus total biomass; and also the intercepts of the relationships of root biomass versus total biomass, plant height versus total biomass and reproductive versus vegetative biomass. At any given plant body size, wild C. teeta has higher biomass in the leaf and less in root than cultivated counterpart. But the trajectories of log rhizome biomass versus log total biomass were the same for both cultivated and wild individuals. Thus, we conclude that (1) the allometric trajectories change at different scales and (2) C. teeta -based agroforestry system could ensure sustainable use of this medicinal plant because of the stable biomass allocation to the salable rhizome organs.
    Print ISSN: 0167-4366
    Electronic ISSN: 1572-9680
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 4
    Publication Date: 2014-12-31
    Description: A pressing challenge facing poor farmers is how to maintain yields in swidden-fallow systems when confronting growing land scarcity and declining soil fertility. The objective of this research is to document the innovative use of biochar and biochar-rich kiln soils on charcoal kiln sites by Amazonian peasant farmers for annual and perennial crop production as part of their swidden-fallow agroforestry cycle. The study was undertaken in a riverside community near Iquitos, Peru, where the availability of primary forest land has decreased significantly over the past 30 years. Charcoal production is a long-standing, near ubiquitous local activity, drawing on wood primarily from secondary forest fallows. Data were collected in 2011 through household interviews (n = 36) and an extensive survey of upland kiln sites (n = 500). Results indicate this innovation, dubbed “kiln site agriculture” (KSA), evolved endogenously within the study community as an adaptation to growing land scarcity. Current landholdings were found to negatively correlate with both the number of crops households (n = 32) cultivated per kiln site ( r  = −0.3483, p  = 0.0254) and the proportion of those sites cultivated with manioc, the local staple crop ( r  = −0.5441, p  = 0.0006), suggesting that land-poor households rely on KSA harvests to supplement subsistence. This study provides evidence charcoal production need not be a rapacious forest use and can, through KSA and biochar, offer an affordable opportunity to peasant farmers who practice swidden-fallow agroforestry where new land in primary forest is scarce and the productivity of their weathered soils is falling.
    Print ISSN: 0167-4366
    Electronic ISSN: 1572-9680
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 5
    Publication Date: 2014-12-31
    Description: Nanoscience emerged in the late 1980s and is developed and applied in China since the middle of the 1990s. Although nanotechnologies have been less developed in agronomy than other disciplines, due to less investment, nanotechnologies have the potential to improve agricultural production. Here, we review more than 200 reports involving nanoscience in agriculture, livestock, and aquaculture. The major points are as follows: (1) nanotechnologies used for seeds and water improved plant germination, growth, yield, and quality. (2) Nanotechnologies could increase the storage period for vegetables and fruits. (3) For livestock and poultry breeding, nanotechnologies improved animals immunity, oxidation resistance, and production and decreased antibiotic use and manure odor. For instance, the average daily gain of pig increased by 9.9–15.3 %, the ratio of feedstuff to weight decreased by 7.5–10.3 %, and the diarrhea rate decreased by 55.6–66.7 %. (4) Nanotechnologies for water disinfection in fishpond increased water quality and increased yields and survivals of fish and prawn. (5) Nanotechnologies for pesticides increased pesticide performance threefold and reduced cost by 50 %. (6) Nano urea increased the agronomic efficiency of nitrogen fertilization by 44.5 % and the grain yield by 10.2 %, versus normal urea. (7) Nanotechnologies are widely used for rapid detection and diagnosis, notably for clinical examination, food safety testing, and animal epidemic surveillance. (8) Nanotechnologies may also have adverse effects that are so far not well known.
    Print ISSN: 1774-0746
    Electronic ISSN: 1773-0155
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 6
    Publication Date: 2014-12-31
    Description: The nematode Radopholus similis is a major pest in banana plantations worldwide. This nematode is actually controlled using synthetic, toxic nematicides. Alternative control methods are therefore needed. For instance the liquid by-product of fiber extraction from sisal ( Agave sisalana ) may be used as a nematicide. Here we tested the nematicidal activity of the sisal residue, fresh or fermented, on R. similis in banana plants. We measured immobility and mortality effects by nematode immersion in an aqueous solution of sisal residue for 24 and 48 h. Nematode control was also evaluated in the Grand Naine banana plants under greenhouse conditions using soil amendments of residues. We measured plant growth, pseudostem diameter, the number of leaves, and the dry weight of the aerial parts, corm and roots, as well as factors related to nematode control such as the level of damage, the population of R. similis in roots and soil, and nematode reproduction factors. Our results show that the sisal residue efficiently controlled R. similis in vitro, displaying mortality rates of 99.2 % for the fresh residue. The damage caused by R. similis on plants was similar for the treatment with the sisal residue at a concentration of 25 % and with the nematicide. This is the first report on the nematicidal effect of the sisal liquid residue on the banana burrowing nematode. This by-product presents the potential for the development of new alternatives for nematode control, with a low-cost and low-environmental risk plant nematicide.
    Print ISSN: 1774-0746
    Electronic ISSN: 1773-0155
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 7
    Publication Date: 2014-12-31
    Description: We describe a simple, efficient process for the production of 6-kestose, a trisaccharide with well-documented prebiotic properties. A key factor is the use of a yeast transformant expressing an engineered version of Saccharomyces invertase with enhanced transfructosylating activity. When the yeast transformant was grown with 30 % sucrose as the carbon source, 6-kestose accumulated up to ca. 100 g/L in the culture medium. The 6-kestose yield was significantly enhanced (up to 200 g/L) using a two-stage process carried out in the same flask. In the first stage, the culture was grown in 30 % sucrose at physiological temperature (30 °C) to allow overexpression of the invertase. In the second stage, sucrose was added to the culture at high concentration (60 %) and the temperature shifted to 50 °C. In both cases, 6-kestose was synthesized with high specificity, representing more than 95 % of total FOS.
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 8
    Publication Date: 2014-12-31
    Description: The potential of a bioscrubber composed of a packed bed absorption column coupled to a stirred tank denitrification bioreactor (STR) was assessed for 95 days for the continuous abatement of a diluted air emission of N 2 O at different liquid recycling velocities. N 2 O removal efficiencies of up to 40 ± 1 % were achieved at the highest recirculation velocity (8 m h −1 ) at an empty bed residence time of 3 min using a synthetic air emission containing N 2 O at 104 ± 12 ppm v . N 2 O was absorbed in the packed bed column and further reduced in the STR at efficiencies 〉80 % using methanol as electron donor. The long-term operation of the bioscrubber suggested that the specialized N 2 O degrading community established was not able to use N 2 O as nitrogen source. Additional nitrification assays showed that the activated sludge used as inoculum was not capable of aerobically oxidizing N 2 O to nitrate or nitrite, regardless of the inorganic carbon concentration tested. Denitrification assays confirmed the ability of non-acclimated activated sludge to readily denitrify N 2 O at a specific rate of 3.9 mg N 2 O g VSS h -1 using methanol as electron donor. This study constitutes, to the best of our knowledge, the first systematic assessment of the continuous abatement of N 2 O in air emission. A characterization of the structure of the microbial population in the absorption column by DGGE-sequencing revealed a high microbial diversity and the presence of heterotrophic denitrifying methylotrophs.
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 9
    Publication Date: 2014-12-31
    Description: This study was undertaken to evaluate genotoxic potential of Thermopsis turcica aqueous extracts on the roots of onion bulb ( Allium cepa L.) by comet assay and random amplified polymorphic DNA technique. The Allium root growth inhibition test indicated that the EC 50 and 2×EC 50 values were 8 and 16 mg/ml concentrations of T. turcica aqueous extracts, respectively. The negative control (distilled water), positive control (methyl methane sulfonate, 10 mg/l) and 8 and 16 mg/ml concentrations of T. turcica extracts were introduced to the roots of onion bulbs for 24 and 96 h. The root growth, DNA damage in root cells and randomly amplified polymorphic DNA (RAPD) profiles of root tissue were used as endpoints of the genotoxicity. The comet assay clearly indicated that dose-dependent single strand DNA breaks in the root nuclei of onions were determined for the treatment concentrations of T. turcica extracts. In comparison to RAPD profile of negative control group, RAPD polymorphisms became evident as disappearance and/or appearance of RAPD bands in treated roots. The diagnostic and phenetic numerical analyses of RAPD profiles obviously indicated dose-dependent genotoxicity induced by Thermopsis extracts. In conclusion, the results clearly indicated that water extract of T. turcica has genotoxic potential on the roots of onion bulbs as shown by comet assay and RAPD technique.
    Print ISSN: 0920-9069
    Electronic ISSN: 1573-0778
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
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  • 10
    Publication Date: 2014-12-31
    Description: Energy & Fuels DOI: 10.1021/ef502122w
    Print ISSN: 0887-0624
    Electronic ISSN: 1520-5029
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
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