Publikationsdatum:
2012-07-31
Beschreibung:
The jumonji (JMJ) family of histone demethylases are Fe2+- and alpha-ketoglutarate-dependent oxygenases that are essential components of regulatory transcriptional chromatin complexes. These enzymes demethylate lysine residues in histones in a methylation-state and sequence-specific context. Considerable effort has been devoted to gaining a mechanistic understanding of the roles of histone lysine demethylases in eukaryotic transcription, genome integrity and epigenetic inheritance, as well as in development, physiology and disease. However, because of the absence of any selective inhibitors, the relevance of the demethylase activity of JMJ enzymes in regulating cellular responses remains poorly understood. Here we present a structure-guided small-molecule and chemoproteomics approach to elucidating the functional role of the H3K27me3-specific demethylase subfamily (KDM6 subfamily members JMJD3 and UTX). The liganded structures of human and mouse JMJD3 provide novel insight into the specificity determinants for cofactor, substrate and inhibitor recognition by the KDM6 subfamily of demethylases. We exploited these structural features to generate the first small-molecule catalytic site inhibitor that is selective for the H3K27me3-specific JMJ subfamily. We demonstrate that this inhibitor binds in a novel manner and reduces lipopolysaccharide-induced proinflammatory cytokine production by human primary macrophages, a process that depends on both JMJD3 and UTX. Our results resolve the ambiguity associated with the catalytic function of H3K27-specific JMJs in regulating disease-relevant inflammatory responses and provide encouragement for designing small-molecule inhibitors to allow selective pharmacological intervention across the JMJ family.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691848/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4691848/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kruidenier, Laurens -- Chung, Chun-wa -- Cheng, Zhongjun -- Liddle, John -- Che, KaHing -- Joberty, Gerard -- Bantscheff, Marcus -- Bountra, Chas -- Bridges, Angela -- Diallo, Hawa -- Eberhard, Dirk -- Hutchinson, Sue -- Jones, Emma -- Katso, Roy -- Leveridge, Melanie -- Mander, Palwinder K -- Mosley, Julie -- Ramirez-Molina, Cesar -- Rowland, Paul -- Schofield, Christopher J -- Sheppard, Robert J -- Smith, Julia E -- Swales, Catherine -- Tanner, Robert -- Thomas, Pamela -- Tumber, Anthony -- Drewes, Gerard -- Oppermann, Udo -- Patel, Dinshaw J -- Lee, Kevin -- Wilson, David M -- 092809/Wellcome Trust/United Kingdom -- 18358/Arthritis Research UK/United Kingdom -- P30 CA008748/CA/NCI NIH HHS/ -- Canadian Institutes of Health Research/Canada -- Wellcome Trust/United Kingdom -- England -- Nature. 2012 Aug 16;488(7411):404-8. doi: 10.1038/nature11262.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Epinova DPU, Immuno-Inflammation Therapy Area, GlaxoSmithKline R&D, Medicines Research Centre, Stevenage SG1 2NY, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22842901" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Amino Acid Sequence
;
Animals
;
Biocatalysis/drug effects
;
Catalytic Domain
;
Cells, Cultured
;
Enzyme Inhibitors/metabolism/*pharmacology
;
Evolution, Molecular
;
Histones/chemistry/metabolism
;
Humans
;
Inhibitory Concentration 50
;
Jumonji Domain-Containing Histone Demethylases/*antagonists &
;
inhibitors/chemistry/classification/metabolism
;
Lysine/metabolism
;
Macrophages/*drug effects/enzymology/*immunology/metabolism
;
Methylation/drug effects
;
Mice
;
Models, Molecular
;
Substrate Specificity
;
Tumor Necrosis Factor-alpha/biosynthesis
Print ISSN:
0028-0836
Digitale ISSN:
1476-4687
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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