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Reply to Karapetyan: Viral synthesis and assembly is unlikely to occur under cell-free PMCA conditions [Letters (Online Only)] (2012)

Chesebro, B., Klingeborn, M., Race, B.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-02-22

Description: The letter to the editor by Karapetyan (1) takes issue with our conclusion in PNAS (2) that the generation of transmissible spongiform encephalopathy (TSE) infectivity in cell-free conditions is against the concept that the TSE infectious agent is a virus. Karapetyan's main point is that poliovirus has been shown to “replicate in a cell-free system” by Wimmer and colleagues (3). We would disagree with the interpretation that these poliovirus experiments are analogous to our protein misfolding cyclic amplification (PMCA) reactions. The divalent cations (Mg2+) and energy sources (ATP and GTP) required for synthesis of viral proteins and nucleic acids, as...

Keywords: Letters

Print ISSN: 0027-8424

Electronic ISSN: 1091-6490

Topics: Biology , Medicine , Natural Sciences in General

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Earthquake triggering long-term carbon storage [Physical Sciences] (2012)

Zoback, M. D., Gorelick, S. M.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-12-27

Description: Juanes et al. (1) offer three specific arguments in response to our finding that “because even small to moderate-sized earthquakes threaten the seal integrity of CO2 repositories… large-scale [carbon capture and storage (CCS)] is a risky, and likely unsuccessful, strategy for significantly reducing greenhouse gas emissions” (2). We believe all...

Keywords: Letters

Print ISSN: 0027-8424

Electronic ISSN: 1091-6490

Topics: Biology , Medicine , Natural Sciences in General

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Fluid migration from the Marcellus is possible [Physical Sciences] (2012)

Warner, N. R., Jackson, R. B., Darrah, T. H., Osborn, S. G., Down, A., Zhao, K., White, A., Vengosh, A.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-12-27

Description: Engelder’s letter (1) argues that the sequestration of hydraulic fracturing fluids (HFFs) and brines by imbibition and capillary binding seals the Marcellus Formation and precludes the flow of fluids into overlying formations. This apparently conflicts with our study suggesting that natural connectivity exists between the Marcellus Formation and shallow aquifers...

Keywords: Letters

Print ISSN: 0027-8424

Electronic ISSN: 1091-6490

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No evidence that seismicity causes CO2 leakage [Physical Sciences] (2012)

Juanes, R., Hager, B. H., Herzog, H. J.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-12-27

Description: In a recent Perspective (1), Zoback and Gorelick argued that carbon capture and storage (CCS) is likely not a viable strategy for reducing CO2 emissions to the atmosphere. They argued that maps of earthquake epicenters portray earthquakes occurring almost everywhere, suggesting that Earth’s crust is near a critical state, so...

Keywords: Letters

Print ISSN: 0027-8424

Electronic ISSN: 1091-6490

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Do not throw equations out [Biological Sciences] (2012)

Gibbons, J.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-11-08

Description: In a recent PNAS paper (1), Fawcett and Higginson analyze citation data for biological journals and conclude that overall citation rate is reduced by including equations in papers. As a serial offender in this regard, I found the paper interesting, if troubling. Here I show that an alternative analysis reveals...

Keywords: Letters

Print ISSN: 0027-8424

Electronic ISSN: 1091-6490

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Mathematical illiteracy impedes biology progress [Biological Sciences] (2012)

Chitnis, N., Smith, T. A.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-11-08

Description: Fawcett and Higginson (1) have shown that citation rates of biology papers with equations in the main text are lower than those of papers without equations. They claim that this implies “heavy use of equations impedes communication among biologists” and recommend that equations be moved to appendixes to improve citation...

Keywords: Letters

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Taxonomic identity of the 30,000-y-old plant [Letters (Online Only)] (2012)

Oxelman, B., Petri, A., Elven, R., Lazkov, G.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-10-10

Description: Yashina et al. (1) reported successful regeneration of whole, fertile plants of Silene stenophylla Ledeb. from immature fruit tissue of Late Pleistocene age using in vitro tissue culture and clonal micropropagation. However, the plants in figures 2–4 in ref. 1 do not possess the diagnostic characters of S. stenophylla. The...

Keywords: Letters

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On the taxonomic status of regenerated plants [Letters (Online Only)] (2012)

Yashina, S., Gubin, S., Maksimovich, S., Yashina, A., Gakhova, E., Gilichinsky, D.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-10-10

Description: We are grateful to Oxelman et al. (1) for their interest in the problem of taxonomic identification of plants regenerated from the fruit tissues of Late Pleistocene age and plants grown from the seeds of extant species growing in the Kolyma Lowland (2). The authors’ arguments regarding the determination of...

Keywords: Letters

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India-Asia collision timing [Letters (Online Only)] (2012)

Aitchison, J. C., Ali, J. R.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-10-03

Description: The contribution of a previously undescribed model by van Hinsbergen et al. (1) to on-going debate on the India-Asia collision is welcomed and will certainly spur further investigation on this important topic. Recognizing the extent of Greater India (GI) is constrained in Gondwana reconstructions by the Wallaby–Zenith plateaus, van Hinsbergen...

Keywords: Letters

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Reply to Aitchison and Ali [Letters (Online Only)] (2012)

van Hinsbergen, D. J. J., Lippert, P. C., Dupont-Nivet, G., McQuarrie, N., Doubrovine, P. V., Spakman, W., Torsvik, T. H.

National Academy of Sciences

In: PNAS - Proceedings of the National Academy of Sciences

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Publication Date: 2012-10-03

Description: We recently presented a compilation of paleomagnetic data arguing for Cretaceous extension within Greater India. These data imply that a Tibetan Himalayan (TH) microcontinent rifted away from India, opening an oceanic Greater India Basin (GIB) in its wake. Consequently, we postulated a two-stage India-Asia collision at ∼52 and 25–20 Ma...

Keywords: Letters

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