Publication Date:
2006-12-16
Description:
The majority of known Toxoplasma gondii isolates from Europe and North America belong to three clonal lines that differ dramatically in their virulence, depending on the host. To identify the responsible genes, we mapped virulence in F(1) progeny derived from crosses between type II and type III strains, which we introduced into mice. Five virulence (VIR) loci were thus identified, and for two of these, genetic complementation showed that a predicted protein kinase (ROP18 and ROP16, respectively) is the key molecule. Both are hypervariable rhoptry proteins that are secreted into the host cell upon invasion. These results suggest that secreted kinases unique to the Apicomplexa are crucial in the host-pathogen interaction.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646183/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646183/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Saeij, J P J -- Boyle, J P -- Coller, S -- Taylor, S -- Sibley, L D -- Brooke-Powell, E T -- Ajioka, J W -- Boothroyd, J C -- 1R01AI045806-01A1/AI/NIAID NIH HHS/ -- AI05093/AI/NIAID NIH HHS/ -- AI059176/AI/NIAID NIH HHS/ -- AI21423/AI/NIAID NIH HHS/ -- AI30230/AI/NIAID NIH HHS/ -- AI36629/AI/NIAID NIH HHS/ -- AI41014/AI/NIAID NIH HHS/ -- F32AI60306/AI/NIAID NIH HHS/ -- R01 AI021423/AI/NIAID NIH HHS/ -- R01 AI021423-20/AI/NIAID NIH HHS/ -- R01 AI036629/AI/NIAID NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2006 Dec 15;314(5806):1780-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17170306" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Amino Acid Sequence
;
Animals
;
Chromosome Mapping
;
Chromosomes/genetics
;
Crosses, Genetic
;
Female
;
Genes, Protozoan
;
Genetic Complementation Test
;
Mice
;
Mice, Inbred BALB C
;
Mice, Inbred CBA
;
Molecular Sequence Data
;
Oligonucleotide Array Sequence Analysis
;
*Polymorphism, Single Nucleotide
;
Protozoan Proteins/chemistry/*genetics/metabolism
;
Quantitative Trait Loci
;
Toxoplasma/enzymology/*genetics/*pathogenicity
;
Toxoplasmosis, Animal/*parasitology
;
Virulence
;
Virulence Factors/chemistry/*genetics/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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