ALBERT

Description: Fluorescence derivatization provides a means of tracing the dynamics of polysaccharides even in the presence of high concentrations of other organic compounds or salts. A method of labeling polysaccharides with fluoresceinamine was extended to polysaccharides of a wide range of chemical composition, and alternative means of preparation were established for polysaccharides not initially amenable to column chromatography. The polysaccharides were activated with cyanogen bromide, coupled to fluoresceinamine, and separated from unreacted fluorophore via gel filtration chromatography or dialysis. Since the resulting derivatized polysaccharides proved to be stable to further physical and chemical manipulation, methods were also developed for re-activation and labeling with a second fluorophore, as well as for tethering the labeled polysaccharides to agarose beads. As an example of the application of this approach, five distinct fluorescently-labeled polysaccharides (pullulan, laminarin, xylan, chondroitin sulfate, and alginic acid) were used to investigate the activities and structural specificities of extracellular enzymes produced in situ by marine microbial communities, providing a means of measuring specifically the activities of endo-acting extracellular enzymes and avoiding use of low molecular mass substrate proxies. These labeled polysaccharides could be used to explore the dynamics of polysaccharides in other types of complex media, as well as to investigate the activities and specificities of endo-acting enzymes in other systems.

Description: Egg production, egg viability and fecal pellet production were determined for individual Acartia omorii, which were fed diets of two species of diatoms (Skeletonema costatum and Phaeodactylum tricornutum) and three species of dinoflagellates (Scrippsiella trochoidea, Heterocapsa triquetra and Cochlodinium polykrikoides). Diets were analyzed for fatty acid content as an indicator of food quality. Depending on the diet, egg production of A. omorii varied over time, diminishing with some diets (S. trochoidea, C. polykrikoides, P. tricornutum). This rate of reduction was much more rapid for a diet of C. polykrikoides, which caused egg production to decrease to ca. 2.4 eggs f−1 d−1 in only four days. As for all diets, egg viability was high at the beginning but with the C. polykrikoides and P. tricornutum diets, it rapidly decreased with time. Fecal pellet production also varied with time, depending on the diet. Egg production rate was closely correlated with fecal pellet production. There was no direct relationship between egg viability and egg production rate, but both egg production and viability were affected by the nutritional quality of food. Egg viability was also highly dependent on the composition of fatty acids in the eggs. Egg viability showed positive correlation with the ratio of ω3:ω6 groups among egg fatty acids, and negative correlation with the ratio of 20:5 (n−3) : 22:6 (n−3). While comparing several diets, egg production rate was higher on diets (H. triquetra and S. trochoidea) containing ample amounts of essential fatty acids such as 18:4 (n−3) and 22:6 (n−3). The results suggest that fertility of A. omorii was dependent upon the quality of the food, and dinoflagellate diets, with the exception of C. polykrikoides, were preferable to diatom diets.

Description: Recently, researchers have begun to identify the prevalence of trait simplification, loss and reversal across all levels of biological organization. These studies have taken increasingly integrated approaches, incorporating phylogenetic, developmental and molecular methods, in the quest towards understanding the patterns and processes behind evolution in reverse. Here, we highlight the emerging interest in the reversibility of evolution by discussing a spectrum of studies examining both the genotypes and phenotypes of evolution in reverse. These integrative approaches have greatly increased our knowledge of the biological interactions that produce patterns of evolution in reverse and have led to promising new areas of research.

Description: Physical and chemical fluid–rock interactions are implicated in controlling earthquake nucleation and recurrence. In particular, interseismic compaction, sealing and healing of fractured fault rocks can lead to strength recovery and stabilisation of fault zones. In contrast, these same processes can also assist increases in pore fluid pressures and consequent destabilisation of faults. Here, we present high-temperature, hydrothermal experiments designed to assess the evolution of strength of fault zones in previously intact rock, and also characterise the associated changes to porosity and permeability. Cores of Fontainebleau sandstone were initially loaded to failure in a high-pressure gas–medium apparatus. The failed specimens were then hydrothermally reacted at 927°C for variable duration under isostatic conditions, and subsequently re-fractured to determine the ‘interseismic’ strength recovery. In the most extreme case, hydrothermally induced gouge compaction, cementation and crack healing resulted in 75% strength recovery after reaction for 6 h. Isostatic hydrothermal treatment also resulted in dramatic reduction in porosity and permeability. Strength of the fault zone following hydrothermal reaction appears to be closely correlated to porosity, consistent with previous studies on brittle failure of porous aggregates. The experimental results show how hydrothermal reactions in fault zones may lead to two competing, time-dependent effects; fault strengthening due to increased cohesion in the fault zone and fault weakening arising from elevated pore pressures within a well cemented, low-permeability gouge layer.

Description: We report results from time-series decay and sequential leaching experiments of laboratory cultured and coastal plankton to elucidate the mechanisms controlling barite formation in seawater. Batch-cultured diatoms (Stephanopyxis palmerina) and coccolithophorids (Emiliania huxleyi) were let to decay in the dark for 8–10 weeks, suspended in aerated seawater. The development of barite crystals was monitored by Scanning Electron Microscopy (SEM). A similar experiment was conducted with plankton collected during the spring-bloom in Vineyard Sound (MA). In addition to SEM, suspended particles were sequentially leached for Ba (distilled water rinse; 10% (v/v) HNO3 rinse at room temperature; 30% (v/v) HCl at 80°C overnight; 50% (v/v) HNO3 at 80°C overnight) immediately after collection, and after 10-week decay in seawater, in seawater poisoned with HgCl2, and in seawater spiked with 135Ba. Both experiments showed an increase in the number of barite crystals during decay. The spring-bloom plankton had initially a large pool of labile Ba, soluble in distilled water and cold dilute HNO3 that was lost from the plankton after 10-week decay in both axenic and nonaxenic conditions. In contrast, Ba in the decayed plankton samples was predominantly in forms extracted by hot HCl and hot HNO3 acids, which were attributed to presence of barite Ba and refractory organic Ba respectively. The increase in barite crystal counts under a Scanning Electron Microscope (SEM), the increase in HCl extractable Ba relative to organic carbon, and the loss of a large fraction of Ba during plankton decay suggest that living plankton consists of a relatively large pool of labile Ba, which is rapidly released during plankton decomposition and acts as the main source of Ba for barite formation in supersaturated microenvironments. Since mass balance indicates that only a small proportion (2 to 4%) of the labile-Ba pool is converted to barite, the availability of microenvironments that could locally concentrate Ba released by plankton decay seems to be the main limiting factor in barite precipitation.

Description: Dissolved organic matter (DOM) is an important constituent of soil solution that plays a role in many chemical and biological processes in soils. Tremendous strides have been made in the past 25 years to more fully describe the composition of DOM and its role in soil. The papers in this special issue contribute to this literature by both summarizing recent research and reporting on new projects. Based on the papers presented here and others in the literature, I suggest that the following three questions would prove fruitful for future research on DOM in soils: (1) How large are various sources and sinks and how are they controlled?; (2) What is the ecological significance of dissolved organic nitrogen (DON) in comparison to dissolved organic carbon (DOC)?; (3) How are fluxes altered in human-dominated environments? In support of answering these questions, I recommend that emphasis be placed on developing new analytical techniques (isotopic, or detailed characterization of DOM constituents), increasing interchanges between aquatic and terrestrial biogeochemists and developing an integrated conceptual approach to the study of DOM in soils that addresses interactions among solid soil organic matter, microorganisms including fungi and mycorrhizal fungi, and DOM.

Description: In 1996, long-term sealed-hole hydrological observatories with subseafloor temperature and pressure sensors were installed in four cased holes drilled by the Ocean Drilling Program into sedimented young oceanic crust east of the Juan de Fuca Ridge. Data recovered over a year later showed that all four holes displayed temperature profiles indicative of vertical fluid flow immediately prior to their being sealed. Warm water was being produced from basement in two cases, and cool ocean bottom water was being drawn into basement at the others. Linear flow rates of ∼60–200 m/h were estimated from the perturbation of the temperature profiles relative to undisturbed geothermal gradients at the sites. The pressure differentials driving the flow were also measured at the time of the observatory installations, allowing estimates of permeabilities of the upper crustal sections penetrated by the holes. Estimated permeabilities vary systematically with age, ranging from about 10−10 m2 in the youngest site (0.9 Ma) to 10−12 m2 in the oldest site (3.6 Ma), confirming an apparent reduction of permeability with age determined with packer experiments at three of the same sites. Combined with other estimates of permeabilities in the same holes using methods with different scales of investigation, the new permeability estimates also provide evidence for a significant scale dependence of permeability in the upper oceanic crust.

Description: We analyse the cephalopod–parasite systems taking into account the abiotic and biotic factors that may affect the host risk of infection (contact) with parasites. On the basis of 2000 individuals comprising 10 cephalopod species collected at a microgeographic area (Galician waters, NW Spain), an attempt is made to find associations between parasite relative species diversity (RSD) and cephalopod life cycle characteristics. Additionally, an index of similarity and the ratios of adult/immature (A/I) and specialist/generalist (S/G) regarding the parasite condition were used to define cephalopod ecological groupings at those species with similar risk of becoming infected with a given parasite fauna. Results show the existence of three ecological groupings (coastal, intermediate, and nerito-oceanic), which suggest that the ecological niche of a cephalopod species is more important in determining its risk of parasitic infection than is phylogeny.

Description: This paper describes the history of cephalopod fisheries in Chile over the past 40 years. Continuous monitoring of three species was undertaken between 1978 and 1999: Loligo gahi, Dosidicus gigas and Octopus mimus. Total cephalopod catches increased from 69 tonnes in 1978 (0.13% total mollusc catch) to 3503 tonnes in 1996 (3.64%). A maximum haul of 15,169 tonnes was taken in 1992 (11.27% total mollusc catch). Small-scale fisheries accounted for the majority of cephalopod captures and industrial catches were rare. L. gahi is caught year-round mainly in the south of Chile, with maximum catches in summer and autumn. Catches of D. gigas are also year-round, mainly concentrated in central Chile. O. mimus has been fished since 1978, although recorded as Octopus vulgaris until the 1990s. The O. mimus fishery is located on the north coast of Chile, and catches are made for the most of the year. Octopus has been landed in the south of Chile since 1991, and is considered as O. mimus in capture statistics despite the fact that several species are represented, including Enteroctopus megalocyathus.

Description: The Illex illecebrosus fishery in the northwestern Atlantic Ocean is trawl-based. I. illecebrosus normally lives less than 1 year. One option for managing such a short-lived species is the use of catch and effort data obtained from fishing vessels during the fishing season to manage the fishery in real time. Verification of the accuracy of data reports is important. Conceivably, information contained in the data stream of vessel position and time provided by a remote data logger could be used to reconstruct the vessel’s activities to assess the reliability of industry reports. This study describes quantitatively the types of vessel operating activities that take place on a typical I. illecebrosus fishing trip and assesses the possibility of reconstructing these activities reliably from the simple data stream of vessel position and time. Seven activities were identified, six of which occurred commonly: steaming to and from port, searching, towing, set-up time between tows, steaming overnight and laying-to overnight. Processing the catch, as a discrete activity, occurred rarely. Each activity could be characterized in terms of its duration and distance traveled, the average vessel speed, and the tendency for vessel speed to change during the activity. Most activities were conducted in a linear manner. Accordingly, reasonable estimates of the distance and duration of these activities could be obtained simply from the knowledge of the starting and ending position and time. Analysis of search time and subsequent catch revealed that searching did not improve catch. More squid would have been caught had the vessels used this time for towing. Catch per unit effort (CPUE) can be calculated using duration or distance in the denominator. In this set of fishing trips, the two were equivalent. Catch bore a nonlinear relationship with CPUE. In particular, larger catches were associated with incrementally larger CPUEs. The uniqueness of each activity when described by its characteristic speed, duration and distance, and the consistency of these characteristics for each activity between vessels suggests that vessel behavior might be assessed remotely using a time series of position and time. Such a capability might be important in any real-time management plan where industry vessels necessarily must be depended upon for data on catch and CPUE.