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  • Cloning, Molecular
  • Transfection
  • Wiley-Blackwell  (1)
  • American Association of Petroleum Geologists (AAPG)
  • Emerald
  • 1995-1999  (1)
  • 1998  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Isolation of ultrapure supercoiled plasmid-DNA using preparative electrophoresis (1998)
    Weinheim : Wiley-Blackwell
    Electrophoresis 19 (1998), S. 1575-1576 
    Details
    ISSN: 0173-0835
    Keywords: Green fluorescent protein ; Transfection ; Transgenic animals ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A large-scale preparative polyacrylamide gel electrophoresis (PAGE) system for the isolation of high-purity supercoiled plasmid-DNA is described. This method should prove suitable for the isolation of large DNA molecules, either plasmid or linear DNA, that is required for the production of transgenic animals, for instance. The efficiency of the method is illustrated by the isolation of the gene for the green fluorescent protein, cloned into a mammalian expression vector and used for transfection of eukaryotic cells.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150191009
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