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  • Articles  (7)
  • Nodulation  (7)
  • Springer  (7)
  • American Chemical Society
  • Cambridge University Press
  • 1995-1999  (7)
  • 1997  (7)
  • Geosciences  (7)
Collection
  • Articles  (7)
Publisher
  • Springer  (7)
  • American Chemical Society
  • Cambridge University Press
Years
  • 1995-1999  (7)
Year
  • 1
    ISSN: 1432-0789
    Keywords: Key words Fungicide ; Mancozeb ; Rhizobium ; Arachis hypogaea ; Nodulation ; Side effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Information on the compatibility of Rhizobium sp. with seed-protectant chemicals is controversial because of variations in the methods used and the lack of quantitative data. The present study was conducted to determine the influence of the fungicide mancozeb (ethylenebis-dithiocarbamate), at recommended doses, on the growth, survival and symbiotic properties of Rhizobium sp. infecting peanut plants (Arachis hypogaea) under laboratory and field conditions. The results indicated that mancozeb decreased growth in pure culture by 50% of both Rhizobium sp. USDA 3187 and a strain isolated from peanut nodules. However, no differences were found in peanut seed yields under field conditions. These results suggest that the soil environment could reduce the probability of the direct, harmful effects of mancozeb on bacterial growth.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0789
    Keywords: Key wordsCasuarina ; Alnus ; Frankia ; Temperature ; Soil moisture content ; Nodulation ; Inoculum storage ; Axenic cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Comparison of the effects of temperature on the growth in culture (increase in protein) of Frankia showed that three strains isolated from Casuarina were more tolerant of high temperature (45°C) than a strain from Alnus rubra. Optimal temperatures for growth of the Casuarina strains were in the range 25–30°C. Growth of the Alnus strain was good at 25°C but poor at 37°C. High temperatures (35–40°C) during storage for 7 months of these Frankia strains in sand, inoculated initially with liquid culture or with Frankia incorporated into alginate beads and permitted to dry, resulted in substantial loss of infectivity for the host plant species. Loss in infectivity was greater with an Alnus Frankia strain than strains from Casuarina cunninghamiana, C. equisetifolia and C. junghuniana. Three Frankia strains from C. equisetifolia were incorporated into a sand/perlite mixture with three different moisture regimes (field moisture capacity – wet: watered and maintained at field capacity; watered to field capacity but then allowed to dry – moderately wet; or watered to half field capacity and then permitted to dry – dry) and then stored for 12 weeks at 25°C and 35°C. Assessment by the most probable number (MPN) technique of the infectivity of the sand mixture for nodulation of C. equisetifolia showed significant interactions between Frankia strain, temperature and soil moisture content. The infectivity of Frankia strains ORS020607 and UGL020602q was not affected by incubation in wet sand at 25°C but fell by more than half after 12 weeks in moderate and dry conditions. Changes in infectivity were similar when incubation was at 35°C. By contrast, the infectivity of UGL020603q fell substantially under all moisture conditions and at both temperatures. The data show the importance of screening for tolerance of both temperature and moisture content when selecting strains for preparation of inoculum for use in hot climates.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 25 (1997), S. 169-174 
    ISSN: 1432-0789
    Keywords: Key wordsAstragalus cicer ; Nodulation ; DNA ; Milkvetch ; Nitrogen fixation ; Forage legume ; Rhizobium spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In 1993 and 1994, 12 bacterial isolates were isolated from root nodules of cicer milkvetch (Astragalus cicer). In the tests for nodulation of A. cicer by these bacterial isolates, five were found to form hypertrophic structures, while only two formed true nodules. These true nodules were formed in a sterilized soil system. This system might be able to act as a DNA donor to provide residual DNA to other microbes in the soil. The rhizobial isolates were thought to have lost genetic material crucial to nodulation during the isolation process. This hypothesis was supported by an experiment in which isolate B2 was able to nodulate A. cicer in vermiculite culture after being mixed with heat-killed rhizobia, Rhizobium leguminosarum bv. trifolii and R. loti. The nodulation would not occur in vermiculite culture system without the heat-killed rhizobia. Based on the biochemical data, the B2 and 9462L, which formed true nodules with A. cicer, were closely related. The rhizobia type cultures that nodulate A. cicer include Bradyrhizobium japonicum, Rhizobium leguminosarum bv. trifolii, R. leguminosarum bv. viceae, and R. loti. All of these rhizobia were from different cross-inoculation groups. The B2 and 9462L isolates could only nodulate Medicago sativa, Phaseolus vulgaris, and Melilotus officinalis, but not these species within the genus from which they were isolated: Astragalus. The traditional cross-inoculation group concept obviously does not fit well in the classification of rhizobia associated with Astragalus. The rhizobia isolated from A. cicer can be quite different, and the rhizobia able to renodulate A. cicer also quite diverse.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 26 (1997), S. 95-99 
    ISSN: 1432-0789
    Keywords: Key wordsCasuarina equisetifolia ; Vesicular-arbuscular mycorrhiza ; Nodulation ; Flooding ; Adventitious roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of vesicular-arbuscular mycorrhizal (VAM) inoculation on nodulation and growth of flooded Casuarina equisetifolia J.R. and G. First was investigated. Casuarina seedlings were grown in sterile soil inoculated with Glomus clarum Nicolson and Schenick and flooding was imposed for 8 weeks. Mycorrhizal Casuarina seedlings adapted to flooding better than non-inoculated seedlings. This was achieved partly by the greater development of adventitious roots and hypertrophied lenticels which increased oxygen availability, and therefore VAM infection in the upper soil zone. The VAM infection led to the suppression of the accumulation of toxic products of anaerobic respiration, such as ethanol. Mycorrhizal inoculation also prevented the total suppression of nodulation in the flooded plants. Nodulation was observed only in the upper soil zone, and its occurrence made dinitrogen fixation possible. The results suggest that mycorrhizal infection is essential for nodulation of Casuarina in flooded soils and is therefore related to the relatively higher dinitrogen accumulation in Casuarina under anoxic conditions.
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  • 5
    ISSN: 1432-0789
    Keywords: Key wordsBradyrhizobium ; Rhizobium ; Pigeon pea ; Cajanus cajan ; Acetylene reduction assay ; Symbiotic ; effectiveness ; Fast-growing strains ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The comparative symbiotic properties of Rhizobium spp. and Bradyrhizobium spp. strains infecting pigeon pea were evaluated. Bradyrhizobium strains (Cajanus) were found to be superior to Rhizobium strains (Cajanus) and the superiority was ascertained to be due to the higher enzyme activity of the tricarboxylic acid (TCA) cycle in comparison to Rhizobium spp. strains. Moreover, metabolic superiority or rapid growth rate does not necessarily correlate with symbiotic effectiveness. The symbiotic performance of isolates varied with the host cultivar. The dry matter accumulation could be correlated with the total acetylene reduction activities rather than nodule number or nodule fresh weight per plant.
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  • 6
    ISSN: 1432-0789
    Keywords: Key wordsBradyrhizobium japonicum ; Nodulation ; Nitrogen fixation ; Soybean ; Thiram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The fungicide thiram, widely used as a chemical seed protectant, induces a strong inhibition of primary nodulation in the crown zone of soybean roots. The present work reports on the isolation of Bradyrhizobium japonicum strains resistant to thiram, some of which (T3B, A86 and A2) maintained their capacity for nodulation and were still efficient symbionts, but some (A1, C1 and C6) lost the ability to stimulate nodulation. Characterization tests such as growth at different pH, denitrifying ability, salt tolerance, production of siderophores and phosphate solubilization were performed on the resistant strains. Inoculants produced from these strains could be appropriate for use with thiram-treated seeds, without causing a loss of bacteria viability.
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  • 7
    ISSN: 1432-0789
    Keywords: Key words Acetylene reduction assay ; Cellulolytic fungi ; Diazotrophic bacteria ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was performed to evaluate the ability of cellulolytic fungi and wheat straw incorporation to improve the nodulation, growth and nitrogen status of fenugreek grown in saline soils. NaCl addition to the growth medium at rates of 0.5 and 1% strongly decreased the enzymatic activity of the ten tested moulds. Three of these fungi, Aspergillus niger, Chaetomium globosum and Trichoderma harzianum, showed the highest enzymatic activity. The three moulds have the ability to degrade straw in the presence of NaCl and T. harzianum was the best straw degrader. Inoculating the plants with Rhizobium meliloti strain TAL1373 and cellulolytic fungi slightly promoted nodulation, growth and nitrogen accumulation when plants were grown with the addition of 0.5% NaCl when compared to plants inoculated with R. meliloti alone. However, application of wheat straw with cellulolytic fungi significantly enhanced growth, nodulation and nodule efficiency at 0.5 and 1.0% salinity. The greatest values of nodulation and growth parameters were obtained with a straw-Trichoderma harzianum combination. Cellulolytic fungi and wheat straw increased the concentration of Ca, Mg and K in the shoots and roots of plants. The increase in dry matter production and N content was mainly due to improved N2 fixation reflected by enhanced formation and growth of nodules as well as nitrogenase activity.
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