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  • Articles  (6)
  • Aspergillus niger  (6)
  • Springer  (6)
  • American Chemical Society
  • 1995-1999  (6)
  • 1996  (6)
  • Process Engineering, Biotechnology, Nutrition Technology  (6)
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  • Articles  (6)
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  • 1995-1999  (6)
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Topic
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of industrial microbiology and biotechnology 16 (1996), S. 351-353 
    ISSN: 1476-5535
    Keywords: citric acid ; Aspergillus niger ; immobilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus niger was immobilized in cryogels and in conventional gels of polyacrylamide. The growth of cells entrapped in two kinds of gels and the production of citric acid by the immobilized cells were investigated and compared. Cells immobilized in cryogels were more suitable for citric acid production.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0972
    Keywords: Activity ; Aspergillus niger ; CMCase ; polysaccharides ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Removal of non-covalently attached polysaccharides from carboxymethylcellulase (CMCase) of Aspergillus niger improved its activity but decreased its thermostability and protease resistance. The activation energy profile of the hydrolysis of carboxymethylcellulose (CMC) was triphasic with increasing values of 17,-55 and-562 kJ/mol for polysaccharide-free and 19, -21 and -207 kJ/mol for polysaccharide-complexed CMCase. The specificity constant (Vmax/Km) of polysaccharide-free CMCase was 1.41 compared to polysaccharide-complexed CMCase which was only 0.68. The polysaccharide free CMCase had lower thermostability (‘melting point’ = 82°C) and higher protease susceptibility compared to polysaccharide-complexed CMCase (‘melting point’〉100°C).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-0972
    Keywords: Aspergillus niger ; pectinase ; solid state fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Exopectinase (exo-p) and endopectinase (endo-p) production by Aspergillus niger CH4 in solid state culture was studied at initial glucose concentrations of 100, 250, 350 and 450 g/l. The highest activity of exo-p (35 U/g) was produced at 72 and 120 h in the medium containing 100 and 250 g glucose/l, respectively. The maximum endo-p activity (9 U/g) was produced at 72 h in the medium with 250 g glucose/l. The reduction in pectinase production at 350 and 450 g/l initial glucose concentration was due neither to repression of the synthesis of the enzyme nor to the glucose consumption rate of the strain but due to a drastic drop in pH of the medium.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 267-271 
    ISSN: 1573-0972
    Keywords: Amyloglucosidase ; aqueous two-phase system ; Aspergillus niger ; solid-state fermentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Amyloglucosidase (AMG) was produced by Aspergillus niger in solid-state fermentation (SSF), submerged fermentation (SmF) and an aqueous, two-phase system of polyethyleneglycol (PEG) and salt. In SSF, a fed-batch mode of operation gave a yield of 64 U/ml compared with 44 U/ml in batch mode. Similar trends were observed for SmF, where fed-batch cultivation gave a yield of 102 U/ml compared with 66 U/ml in batch. Shorter cultivation times (66 h) were required for SmF than for SSF (96 h). In the aqueous, two-phase cultivation, the productivity and yield of AMG were both twice those in the control fermentation.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 301-302 
    ISSN: 1573-0972
    Keywords: Adenocalymma alliaceum ; Alternaria brassicae ; antifungal activity ; Aspergillus niger ; spore germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A simple and rapid technique is reported for the preliminary screening of fungi-toxic extracts/samples by direct spotting onto silica gel plates and subsequent over-spraying with a fungal spore suspension. After incubation fungi-toxicity is indicated by a growth inhibition zone, the area of which is related to the concentration of the sample.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 655-656 
    ISSN: 1573-0972
    Keywords: Aspergillus niger ; citric acid ; inoculum ; spore viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The type of sporulation medium and time of incubation had an effect on spore viability and citric acid production by mycelia grown from Aspergillus niger spores. Shu & Johnson agar (SJA) and potato dextrose agar gave higher citric acid titres than malt-extract agar. SJA also gave better germinability than the other media. Viability increased with time of incubation, but higher production of citric acid was achieved with spores incubated for less than 7 days.
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