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  • Articles  (2,691)
  • Springer  (2,327)
  • Nature Publishing Company  (364)
  • 1995-1999  (2,691)
  • 1945-1949
  • 1995  (2,691)
  • Process Engineering, Biotechnology, Nutrition Technology  (2,691)
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  • Articles  (2,691)
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  • 1995-1999  (2,691)
  • 1945-1949
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Journal
  • 1
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 936-937 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] In the end, the former chief executive officer(CEO) of Genentech (S. San Francisco, CA),G. Kirk Raab, was not especially unethical or even unacceptably greedy. It was more that he was a throwback to a more freewheeling, entrepreneurial era who was unable to adjust to a more severe business ...
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  • 2
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 940-942 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Since its approval in 1987, Glaxo Wellcome's (London) AZT has been the mainstay of retroviral therapy for patients with HIV infection. AZT was the first of a class of HIV drugs known as reverse transcriptase (RT) inhibitors, which act by blocking the viral enzyme reverse transcriptase. Together ...
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  • 3
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 949-953 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Pharmacoeconomic studies need universal guidelines and harder data if they are to be used realistically to value—and not just ...
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  • 4
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 955-959 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A risk-based proposal for regulating field trials—of any kind of organism—that is adaptable to any form of government regulation or institutional ...
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  • 5
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 962-964 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Many biotech patent applications have experienced delays at the U.S. Patent and Trademark Office (PTO, Crystal City, VA) because of the necessity of demonstrating the utility of the invention. Utility is an absolute requirement for an inventor to receive a granted patent. The statute states, ...
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  • 6
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 968-973 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] New possibilities for the diagnosis and treatment of reproductive and genetic disorders are becoming available as a result of a series of recent technical advances. Intracytoplasmic sperm injection (ICSI) allows treatment of numerous infertile men whose sperm cannot penetrate the egg to initiate ...
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  • 7
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 721-721 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] When it comes to regulatory issues, American politicians are switch-hitters, stepping into the box only after they have sensed which way the winds of political fortune are blowing. It used to be that if you played for the Republicans, you were antiregulatory, antigovernment down the line. Democrats ...
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  • 8
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 722-723 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: In an article1 by this same title, Willem Stemmer of the Affymax Research Institute has made a very good argument for using genetic algorithms (GAs) to search protein sequence space. While I am in agreement about the power of GAs to find solutions to complex problems, I am extremely ...
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  • 9
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 733-733 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] OXFORD, U.K.—Officials of the European Union (EU, Brussels) and of Japan's Ministry of International Trade and Industry (MTTI, Tokyo) have agreed to establish a working group to exchange information about biotechnologypolicy. StefanoMicossi, the director general for industry of the ...
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  • 10
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 734-735 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] This spring, the European Parliament (EP, Strasbourg, France) rejected, by a decisive majority, a directive that would have imposed various uniform standards of biotechnology patent laws on all the member states of the European Union (EU, Brussels). This result came as a surprise to many, since up ...
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  • 11
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 737-739 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] WASHINGTON, D.C.—With the shortage of human donor organs for surgical transplants growing more acute, biomedical researchers are exploring ways to use xenografts—organs from such species as baboons and pigs—to transplant into humans. But to do so, such challenges as immunological ...
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  • 12
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 791-794 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To determine whether targeted DNA insertion into the tobacco plastid genome can be obtained without physical linkage to a selectable marker gene, we carried out biolistic transformation of chloroplasts in tobacco leaf segments with a 1:1 mix of two independently targeted antibiotic resistance ...
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  • 13
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 801-804 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A major antigenic site (site A) of foot-and-mouth disease virus includes multiple overlapping epitopes located within the flexible G-H loop of capsid protein VP1. We have studied the antigenicity of several recombinant E. coli β-galactosidases displaying the site A from a serotype C virus in ...
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  • 14
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    Nature biotechnology 13 (1995), S. 807-807 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Liquid substrate Biotecx Laboratories (Houston, TX) offers One-Blue, a TMB-single liquid substrate for HRP. Tetramethylbenzidine is most commonly used for horseradish peroxidase-based assays. One-Blue contains both TMB and hydrogen peroxide, and in the presence of HRP reacts to produce a blue, ...
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  • 15
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 628-628 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Biotechnology, before it was even called biotechnology, can be said to have begun with the production of penicillin, which initiated the revolution in antibiotics half a century ago. Now that many of those weapons have declined in value because so many bacteria have become invulnerable to them, how ...
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  • 16
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 638-640 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] It has never been easy for a biotechnology company to raise the amount of money it requires—on average, more than $450 million to reach operating profitability—but it has never been more difficult than it is in the current financing environment. The U.S. biotechnology industry ...
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  • 17
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    Nature biotechnology 13 (1995), S. 644-645 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] WASHINGTON, D.C.—The first full DNA sequences of two bacteria have been determined by Craig Venter of The Institute for Genomic Research (TIGR, Gaithersburg, MD) and Hamilton Smith of Johns Hopkins University (Baltimore, MD), the two reported at the 95th General Meeting of the American ...
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  • 18
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 647-651 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Can information from large molecules lead to the design of small molecule ...
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  • 19
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    Nature biotechnology 13 (1995), S. 658-660 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] If you submit a protein sequence to Swiss-Model, it will send you back the protein's predicted structure by e-mail. You don't have to be an expert in protein modeling, and you don't need expensive computer hardware and software. Just as you would take your roll of films to a photoprocessor, so you ...
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  • 20
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    Nature biotechnology 13 (1995), S. 669-673 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Extracellular proteases of the subtilisin-class depend upon calcium for stability. Calcium binding stabilizes these proteins in natural extracellular environments, but is an Achilles' heel in industrial environments which contain high concentrations of metal chelators. Here we direct the evolution ...
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  • 21
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    Nature biotechnology 13 (1995), S. 686-691 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] A 1.1 kb rice genomic DNA fragment, containing a chitinase gene under the control of the CaMV 35S promoter, was cloned into the rice transformation vector pGL2. After transformation of Indica rice protoplasts in the presence of polyethyleneglycol, plants were regenerated. The presence of the ...
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  • 22
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    [s.l.] : Nature Publishing Company
    Nature biotechnology 13 (1995), S. 699-699 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] RT assay New from Amersham Life Science (Little Chalfont, U.K.), Quan-T-RT allows direct detection and quantification of reverse transcriptase (RT) in tissue culture supernatants. Developed for HIV RT, the single-tube assay is based on scintillation proximity assay technology, avoiding sample ...
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  • 23
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    Nature biotechnology 13 (1995), S. 529-529 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Can innovation be managed, in much the same way we like to imagine we are managing information and care? At “Drug Development in the Era of Health Care Reform,” a meeting of pharmaceutical executives sponsored by the Center for the Study of Drug Development last month, the answer would ...
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  • 24
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    Nature biotechnology 13 (1995), S. 530-530 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: The article “Nanotechnology's Many Disciplines” (Bio/Technology 13:439-443, May), by Daniel Thomas, highlights a popular and fruitful theme of recent years, namely the interface of biology, chemistry, and physics at the nanoscale and its many potential applications. ...
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  • 25
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    Nature biotechnology 13 (1995), S. 649-649 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] With increasing pressures to develop cost-effective drugs that deliver real benefit, biotech and pharmaceutical companies alike are eyeing the top ten money-producing drugs as possible candidates for small molecule development (see Table 1). The logic of this approach seems indisputable: Taking a ...
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  • 26
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    Nature biotechnology 13 (1995), S. 656-657 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Recently, the U.S. Court of Appeals for the Federal Circuit1 rendered a decision that could reverse a U.S. Patent and Trademark Office (PTO, Crystal City, VA) policy that has effectively blocked the patenting of many gene sequences. The decision, known as In re Deuel,2 rejects the PTO's ...
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  • 27
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Recombinant human interferon-γ (IFN-γ) was expressed in Chinese hamster ovary cells, baculovirus-infected Sf9 insect cells and the mammary gland of transgenic mice. The N-linked carbohydrate populations associated with both Asn25 and Asn97 glycosylation sites were characterized by ...
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  • 28
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    Nature biotechnology 13 (1995), S. 610-610 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] DNA template isolation The MPG DNA template isolation kit from CPG (Lincoln Park, NJ) provides a fast, reliable means of isolating DNA templates for direct sequencing of PCR fragments. The kit includes streptavidin-coated superpara-magnetic porous glass particles and biotinylated and ...
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  • 29
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    Nature biotechnology 13 (1995), S. 618-618 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Throughout most of its recent history, science was constrained only by the limits of imagination and creativity of its participants. In the past couple of decades that state of affairs has changed dramatically. Science is now constrained primarily by the number of research posts and the amount of ...
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  • 30
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    Nature biotechnology 13 (1995), S. 577-582 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have increased the lysine content in the seeds of canola and soybean plants by circumventing the normal feedback regulation of two enzymes of the biosynthetic pathway, aspartokinase (AK) and dihydrodipicolinic acid synthase (DHDPS). Lysine-feedback-insensitive bacterial DHDPS and AK enzymes ...
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  • 31
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    Nature biotechnology 13 (1995), S. 597-601 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed a fiber-optic chemical sensor for determining dissolved carbon dioxide and assessed its performance for the on-line monitoring of fermentation. The sensor operates on the Severinghaus pCO2 electrode principle; it consists of a pH sensitive dye (hydroxypyrenetrisulfonic acid, HPTS) ...
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  • 32
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have constructed a new adenovirus (Ad) expression vector, pAdBM5, that allows for the production of unprecedented levels of recombinant protein in the human 293 cell line using the Ad expression system. The main feature of this vector is a combination of enhancer sequences that increases the ...
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  • 33
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    Nature biotechnology 13 (1995), S. 417-417 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Fungi, viruses, and other pathogens wreak havoc on the world's bananas and plantains. The banana is a major tropical cash crop, bested only by coffee in the marketplace. And plantains—large cooking bananas—are a food staple in many parts of Central and South America and Africa. While ...
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  • 34
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    Nature biotechnology 13 (1995), S. 425-426 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] OXFORD, U.K.—In the space of a few weeks earlier this year, two of Britain's leading biopharmaceutical companies unveiled disappointing news about their most-advanced products. British Biotech (Oxford) revealed that a manufacturing glitch would result in a delay in the phase HI clinical ...
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  • 35
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    Nature biotechnology 13 (1995), S. 338-338 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The near cartel-like situation in the fetal bovine serum (FBS) market has led to a search for serum equivalents that would theoretically be free of the myriad problems associated with it. Serum equivalent promoters, such as Dr. Richard Ham, scientific advisor to Clonetics (San Diego, CA), believe ...
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  • 36
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    Nature biotechnology 13 (1995), S. 373-377 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Calmodulin is a highly acidic protein (net charge -24 at pH 8.0 in the absence of calcium) that binds to peptide and organic ligands with high affinity (Ka 〉 109 M-1) in a calcium-dependent manner. We have exploited these properties to develop calmodulin as a versatile tag for antibody ...
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  • 37
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed a system for probing protein/protein interactions which makes use of the bacterial flagellum to display random peptide libraries on the surface of E. coli. In developing the system the entire coding sequence of E. coli thioredoxin (trxA) was inserted into a dispensable region of ...
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  • 38
    ISSN: 1546-1696
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    Notes: [Auszug] We describe a novel phage display system that affords the surface expression and hence affinity selection of cDNAs. The strategy is based on a new approach to functionally display proteins on filamentous phage through the attachment to the C-terminus of the minor coat protein VI. The utility of the ...
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  • 39
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    Nature biotechnology 13 (1995), S. 512-512 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Flexible pipettes The new Corning polyethylene flexible pipettes, from Bibby Sterilin (Stone, U.K.), are designed to reach deep into sample containers—even the most difficult shapes—to allow for safe handling of hazardous cultures. The two sizes (15 and 28 cm) are gamma-irradiated to ...
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    Nature biotechnology 13 (1995), S. 306-306 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: It is hard to see how the physicochemical approach of Irena Cosic could work, at least on the basis of the information as presented in the article “Virtual Spectroscopy for Fun and Profit” (Bio/Technology 13:236-238, February). The method plots protein sequence profiles ...
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    Nature biotechnology 13 (1995), S. 311-312 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] OXFORD, U.K.—European biotechnology is gathering momentum, and Germany—contrary to perceived wisdom—is emerging as a hothouse for new startups. These are the main conclusions of Ernst & Young's (E&Y, the Hague) second annual report on the European biotech industry, which ...
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  • 42
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    Nature biotechnology 13 (1995), S. 201-201 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Margaret Mellon and Jane Rissler reply: Miller argues for the safety of transgenics by asserting that “an individual breeder of corn, soybean, wheat, or potato may introduce…50,000 new genotypes per year” without creating unmanageable problems. This argument considerably ...
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    Nature biotechnology 13 (1995), S. 327-329 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Fooling the catalytic RNA subunit into recognizing nonnatural substrates may have important implications for ...
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  • 44
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    Nature biotechnology 13 (1995), S. 341-341 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Serum-supplemented media (SSM) can present practical disadvantages, such as the presence of inhibitors and/or stimulants of growth/cellular functions or the difficulty/increased cost of purifying product. Serum-free media (SFM) can provide a more defined, controlled cell culture environment. ...
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    Nature biotechnology 13 (1995), S. 313-313 
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    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Oh, for the good old days! Time was when being a biotech chief executive officer (CEO) was an enviable position. To marshall cutting-edge science in search of cures for human misery. To dream of Nobel laureates wandering the halls engaged in stratospheric discourse. These were the expectations of ...
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  • 46
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    Nature biotechnology 13 (1995), S. 319-320 
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    Notes: [Auszug] Advanced diagnostic imaging—which consists of nuclear medicine, magnetic resonance imaging (MRI), computerized tomography (CT), X-ray examinations, and ultrasound—is performed 84 million times each year in the U.S. In addition to enhancing the diagnostic confidence of disease detection, ...
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    Nature biotechnology 13 (1995), S. 322-322 
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    Notes: [Auszug] Now that biotech companies are finally starting to enjoy sales they have to resolve some crucial commercial questions, including inventory turns, receivables management, and, yes, even compensation for salespeople. Even though incentives for salespeople are nagging puzzles for even the best ...
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    Nature biotechnology 13 (1995), S. 199-199 
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    Notes: [Auszug] February 4, the day before the 1995 Miami Bio/Technology Winter Symposium on Protein Engineering and Structural Biology began, was a landmark day for meteorology, as dire theoretical predictions of winter storms and arctic fronts were experimentally vindicated. Although a few featured speakers were ...
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    Nature biotechnology 13 (1995), S. 334-334 
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    Notes: [Auszug] FBS severely tests the limits of available viral detection technology. These technological solutions tend to deny the sera of the very factors that make it sought-after, and can often significantly affect the serum nutrient quality. With almost three dozen tests run on what is perhaps the world's ...
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    Nature biotechnology 13 (1995), S. 394-394 
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    Notes: [Auszug] Size analyzer The LS 100Q laser diffraction particle size analyzer from Coulter (Hi-aleah, FL) is fully automated and designed for quality control. It features a range of 0.4-1000 μm (in particle diameter) and Windows-based software, including a statistics package and compare-to-standard ...
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    Nature biotechnology 13 (1995), S. 212-212 
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    Notes: [Auszug] OXFORD, U.K.—The European Union (EU, Brussels) is putting out mixed signals as far as biotechnology goes. On the positive side, the EU recently cobbled together an eleventh-hour compromise on its directive on biotech patents, saving the directive from extinction. On the negative side, though, ...
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    Nature biotechnology 13 (1995), S. 106-107 
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    Notes: [Auszug] FORT COLLINS, Colo.—If the last quarter of 1994 is an indication of things to come, 1995 promises to be a busy one for Cecil Pickett, executive vice president of research operations at Schering-Plough (Madison, NJ). Only a little more than a year on the job, Pickett, who moved from a similar ...
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    Nature biotechnology 13 (1995), S. 200-201 
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    Notes: [Auszug] To the editor: The answer to the question raised in your editorial “Are Biotech Companies Obsolete?” (Bio/Technology 11:1047, November) is an emphatic no. The role of the biotech company in drug development is greater than ever—and growing every day. Entrepreneurial biotech ...
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    Nature biotechnology 13 (1995), S. 231-233 
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    Notes: [Auszug] Maynard Olson has very definite views on the status of DNA sequence technology in relation to the demands of the human genome program. “Efforts to automate conventional technology have almost totally failed⃜What is currently called “automation” involves the use of alleged ...
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    Nature biotechnology 13 (1995), S. 211-212 
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    Notes: [Auszug] It's called “industry on a page.” It's also hailed as misleadingly simple and absolutely brilliant in the same breath. Starting in the late 1970s, Michael Porter of Harvard Business School (Boston, MA) devised a simple diagrammatic method to understand almost any complex industry. The ...
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    Nature biotechnology 13 (1995), S. 261-264 
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    Notes: [Auszug] A hybrid recombinant baculovirus-bacteriophage T7 expression system was developed for transient expression in insect cells of plasmids with foreign genes provided with a T7 promoter. The coding sequence for T7 RNA polymerase, with or without a nuclear localization signal, was inserted into the ...
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    Nature biotechnology 13 (1995), S. 280-280 
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    Notes: [Auszug] Crystal clear Camlab's (Cambridge, U.K.) new Crystal Clear range of beakers and cylinders, made from poly methylpentane (PMP), are virtually unbreakable during regular use. Withstanding temperatures between −150° C and +175° C, and autoclavable at 121° C, they offer high-clarity ...
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    Nature biotechnology 13 (1995), S. 105-105 
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    Notes: [Auszug] To the editor: I would like to add a couple of points to Stephen Edgington's excellent exposé of environmental biotechnology (Bio/Technology 12: 1338–1342, December). First, though gene technology is making exciting contributions to environmental biotechnology, such as the tailor-made ...
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    Nature biotechnology 13 (1995), S. 183-183 
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    Notes: [Auszug] Meetings Feb. 13-18. American Association for Cancer Research meeting on the molecular biology of cancer: Implications for prevention and therapy. Maui, HI. Tel: 1 215 440 9300. Fax: 1 215 440 9313. Feb. 19-23.BIOEXPO '95. The 6th exhibition of biotechnology applied to research, industry, and ...
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    Nature biotechnology 13 (1995), S. 1424-1424 
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    Notes: [Auszug] Campaigns against new developments in science and technology can, paradoxically, help to promote them, as Mike Ward commented recently in Bio/Technology (13:1048). Citing Martin Bauer of the London School of Economics and the coauthors of Resistance to New Technologies (Cambridge University Press, ...
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    Nature biotechnology 13 (1995), S. 107-107 
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    Notes: [Auszug] NEW YORK—Over the past year or so, Ligand Pharmaceuticals (San Diego, CA) has bravely battled the storm that has battered the biotech sector. Its most recent effort is a joint venture with Allergan (Irvine, CA) to form Allergan Ligand Retinoid Therapeutics (ALRT), which will focus on die ...
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    Notes: [Auszug] The preparation of pure and homogeneous membrane proteins or membrane protein complexes is time consuming, and the yields are frequently insufficient for structural studies. To circumvent these problems we established an indirect immunoaffinity chromatography method based on engineered Fv ...
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    Nature biotechnology 13 (1995), S. 180-180 
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    Notes: [Auszug] dsDNA analysis Beckman's (High Wycombe, U.K.) eCAP dsDNA 1000 Kit, for use with the P/ACE capillary electrophoresis system, simplifies the analysis of double-stranded DNA. A standard restriction fragment analysis takes only 12 minutes and eliminates slab-gels. PCR products can be analyzed directly ...
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    Nature biotechnology 13 (1995), S. 181-182 
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    Notes: [Auszug] Ultramicro cells Hellma Cells (Forest Hills, NY) offers a series of four cells designed to facilitate the spectrophotomeric analysis of minute volumes. Small volumes can be filled and emptied through the filling/emptying ports by using a pipette and suitable tips when the sample is slightly larger ...
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    Nature biotechnology 13 (1995), S. 1452-1457 
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    Notes: [Auszug] Transgenic animals are a useful in vivo experimental model for assessing the ability and impact of foreign gene expression in a biological system. Transgenic mice are most commonly used, while transgenic steep, goats, pigs and cows have also been developed for specific, “applied” ...
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    Nature biotechnology 13 (1995), S. 1474-1478 
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    Notes: [Auszug] Protein-protein interactions are often dependent on the post-translational modification of one component of a complex. To facilitate the study of these interactions in signal transduction, we have developed the yeast tribrid system, a modification of the yeast two-hybrid system. We demonstrate that ...
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    Notes: [Auszug] A genetically modified microorganism (GMM), Pseudomonas fluorescens SBW25 (lacZY and kanr-xylE), derived from a strain taken from the leaves of mature sugar beet (Beta vulgaris var. Amethyst), was released as a seed inoculum (ca 1×107 cfu/seed) to field-grown sugar beet in 1993 and 1994. The ...
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    Nature biotechnology 13 (1995), S. 1498-1503 
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    Notes: [Auszug] An electrophoretic procedure based on isotachophoresis has been developed for protein purification on a preparative scale in the 10 to 500 mg range. The system is simple, uses well understood physical properties, does not need ampholyte spacers and is able to produce sterile products of clinical ...
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    Notes: [Auszug] A general method for expression, purification, immobilization, detection and radiolabeling of extracellular domains (ECD) of type I membrane proteins. The type I interleukin-1 receptor (IL-lRtI), the α-subunit of interleukin-2 receptor (IL-2Rα) and E-selectin are used as illustrative ...
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    Nature biotechnology 13 (1995), S. 1236-1236 
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    Notes: [Auszug] Fluorescent illumination The Polychrome I from Applied Scientific Instrumentation, Inc. (Eugene, OR) is a high-speed, polychromatic illumination system for fluorescent microscopy. The unit consists of a galvanometer-based monochromator with a Xenon light source, drive electronics, and quartz fiber ...
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    Nature biotechnology 13 (1995), S. 1030-1030 
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    Notes: [Auszug] To the editor: In a special section on regional development (Bio/Technology 13:763-64), Sinja Sveinsdottir envisions a bright future (“Medicon Valley”) for the biotechnology industry in the Øresund area following the construction of a bridge between Sweden and Denmark. Working ...
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    Nature biotechnology 13 (1995), S. 1031-1031 
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    Notes: [Auszug] Due to a printing error, Figure 4 in Collins-Racie, LA., McColgan, JM, Grant, K.L, DiBlasio-Smith, E.A., McCoy, J.M., and LaVallie, E.R. 1995. Production of recombinant bovine enterokinase catalytic subunit in Escherichia coli using the novel secretory fusion partner DsbA. Bio/Technology 13:982-987 ...
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    Nature biotechnology 13 (1995), S. 1036-1036 
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    Notes: [Auszug] A “transgenic arthropod team” has been formed in the Biotechnology, Biologies, and Environmental Protection division of the U.S. Department of Agriculture's Animal and Plant Health Inspection Service (APHIS-BBEP; Washington, D.C.). According to Orrey Young at APHIS, the agency has ...
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    Nature biotechnology 13 (1995), S. 1043-1045 
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    Notes: [Auszug] Not long after the November 1994 elections in the U.S., a conservative juggernaut with the new House of Representative speaker, Newt Gingrich, at the lead, hit Washington like a runaway freight train. The U.S. Job Creation and Wage Enhancement Act, perhaps the most telling—and ...
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    Nature biotechnology 13 (1995), S. 1056-1058 
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    Notes: [Auszug] The merging of molecular biology with biosensors and electronics promises to reshape ...
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    Nature biotechnology 13 (1995), S. 1029-1029 
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    Notes: [Auszug] The week in mid-September that Jeremy Rifkin and the “coalition of more than 200” (as the press generally refers to them) filed a challenge against W.R. Grace's pesticide patent on an extract of the neem tree's active ingredient, azadiracthin, was unusually busy in Bangalore. Local ...
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    Nature biotechnology 13 (1995), S. 1030-1031 
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    Notes: [Auszug] To the editor: The contribution by David Goodstein (Bio/ Technology 13:618) raised the issue that “eminent” people in a particular field of science may use their positions in order to maintain their eminence, especially during times of funding shortfalls. He points out that this is ...
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    Nature biotechnology 13 (1995), S. 1031-1031 
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    Notes: [Auszug] To the editor: In the review article by Lisa Evans and Mark Burns on focusing techniques, entitled “Solute Focusing Techniques for Bioseparations,” (Bio/ Technology 13:46-52, January) we have missed a highly cost-effective category for isolation, purification, and concentration of ...
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    Nature biotechnology 13 (1995), S. 1065-1066 
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    Notes: [Auszug] Should a patent owner be able to exclude competitors from testing drugs that might infringe upon their patent until the patent's term is over? The knee-jerk response would seem to be yes: We routinely think of a patent as the means to exclude competitors from commercializing a patented product. But ...
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    Nature biotechnology 13 (1995), S. 67-71 
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    Notes: [Auszug] The spoOA gene of Bacillus subtilis encodes the key factor involved in the initiation of sporulation. It was previously shown that the B. thuringiensis (Bt) cryIIIA gene, encoding a toxin active against coleopteran larvae, is overexpressed in an spoOA mutant of B. subtilis. In this paper we ...
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    Nature biotechnology 13 (1995), S. 90-90 
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    Notes: [Auszug] Modular system Bio-Rad's (Hemel Hempstead, U.K.) BioLogic System gives high-resolution analytical- and preparative-scale purification of proteins and DNA. The workstation operates low and high (up to 1000 psi) pressure columns. Options include additional detectors, manual and automated values, ...
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    Nature biotechnology 13 (1995), S. 7-7 
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    Notes: [Auszug] The U.S. Food and Drug Adminstration (FDA), a federally mandated agency, stands between companies and their profits, the Congress and the White House, congressional members and their constituents, con sumers and their products. It is difficult to imagine a less enviable position. Or a less tenable ...
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    Nature biotechnology 13 (1995), S. 96-96 
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    Notes: [Auszug] As transgenic crops near commercial production, the debate about their environmental safety is intensifying. Some argue that the safety of field trials predicts safety at the commercial scale. No one, however, has evaluated the data from the U.S. Department of Agriculture (USDA, Washington, D.C.) ...
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    Nature biotechnology 13 (1995), S. 1010-1010 
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    Notes: [Auszug] Similarity searches Time Logic (Moorpark, CA) introduces the DeCypher II, which performs sensitive similarity searches of genetic data banks. The sequence similarity engine (SSE) hardware becomes the algorithm, and performance scales in proportion to the number of SSE cards installed. Anumber of ...
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    Nature biotechnology 13 (1995), S. 1009-1009 
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    Notes: [Auszug] Bacteria barrier Porex Technologies (Fairburn, GA) has developed the Porex T3 bacteria barrier sheet with a 99.8% bacterial filtration efficiency. The sheet has a thickness ranging from 0.025–0.25 inches and a median pore size of 7μm. The sheet is hydrophobic by nature, but can be made ...
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    Notes: [Auszug] We introduced a firefly luciferase gene, expressed under control of Drosophila heat shock protein gene promoter, into Autographa californica nuclear polyhedrosis virus (AcNPV). When the 5th instar larvae of the silkworm, Bombyx mori, were inoculated with the recombinant virus, luciferase activities ...
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    Nature biotechnology 13 (1995), S. 1022-1022 
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    Notes: [Auszug] As more biotechnology companies reach profitability, more of you will start considering ways in which you can contribute to your community in a way that reflects the culture of your company and the needs of our industry. As you do so, I recommend you consider two factors. First, evaluate what your ...
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    Nature biotechnology 13 (1995), S. 925-925 
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    Notes: [Auszug] It is becoming more and more possible to manipulate the human reproductive process to produce healthy babies on demand, and to treat sick fetuses early in development. As evidenced by the review from Gene Levinson and his colleagues in this issue, ingenious remedies for infertility and ...
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    Nature biotechnology 13 (1995), S. 926-926 
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    Notes: [Auszug] To the editor: We would like to thank Bernard Dixon for his kind words about our work in his commentary in the April issue of Bio/Technology(13:308). After dealing with the noted frustrations for several years, it is good to have the supporting data. We are still frustrated, however. Ironically, ...
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    Nature biotechnology 13 (1995), S. 926-927 
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    Notes: [Auszug] John Hodgson's article “To Treat or Not To Treat” (Bio/Technology 13:333-343) was just as excellent as its two predecessors, “Checking Sources: The Serum Supply Secret” {Bio/Technology 9:1320-1324, 1991) and “Fetal Bovine Serum Revisited”(Bio/Technology 11:49-53, ...
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    Nature biotechnology 13 (1995), S. 927-927 
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    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] In his letter (Bio/Technology 7:626), Ole B. Nielsen referred to the four USDA classifications of fetal bovine serum sources. Specifically, they are: class I (free importation)—New Zealand/Scandinavia; class II (domestic)—U.S./Canada; class III (safety testing ...
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    Nature biotechnology 13 (1995), S. 932-933 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] OXFORD, U.K.—Japanese companies are planning to step up their global biotech activities, including the establishment of partnerships with foreign firms and the setting up of offshore subsidiaries. More than 66% of Japanese multinationals responding to a recent survey conducted by BioBusiness ...
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  • 93
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    Nature biotechnology 13 (1995), S. 928-928 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] You are working in a small, poor country. Over the last two years you have developed an ingenious and remarkably inexpensive test for the differentiation of arboviruses. It takes slightly longer than existing procedures, and does not require the sophisticated reagents that are available in ...
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  • 94
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    Nature biotechnology 13 (1995), S. 933-933 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] ALLENTOWN, Penn.—The U.S. market for skin-replacement products designed to treat severe bums and chronic wounds could be heating up. Currently, two such products are on the market, one produced by Genzyme Tissue Repair (GTR, Cambridge, MA) and the other by LifeCell (The Woodlands, TX). Also, ...
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  • 95
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    Nature biotechnology 13 (1995), S. 426-427 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] For the last two to three years, consolidation has been a subject of intense attention within the biotechnology industry. Proponents and opponents alike seem to agree that consolidation is inevitable. The arguments are often very similar: There are too many biotech companies or with the ...
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  • 96
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    Nature biotechnology 13 (1995), S. 434-435 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] One year ago, leading environmentalists, emboldened by the apparent ambivalence of the Clinton administration, called for an indefinite moratorium on the commercialization of transgenic plants, particularly those engineered with natural and synthetic toxins for protection from crop pests and ...
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  • 97
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    Nature biotechnology 13 (1995), S. 442-442 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] “Nanotechnology is an explosive technology that will see far greater application in biological research,” So says Gerald Pollack at the Bioengineering Center of the University of Washington (Seattle, WA). Pollack and his colleague, MarkFauver, along with other collaborators, have ...
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  • 98
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    Nature biotechnology 13 (1995), S. 460-462 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] By now, nearly all inventors are aware that on December 8,1994, President Clinton signed into law the Uruguay Round Agreements Act (URAA). The “GATT bill,” as it is often called, expands and revises the General Agreement on Tariffs and Trade (GATT, Geneva) and implements a number of ...
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  • 99
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of ...
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  • 100
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    Nature biotechnology 13 (1995), S. 504-506 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Preparations of rHMfA (recombinant histone A from Methanothermus fervidus) synthesized in E. coli by the heterologous expression of the hmfA gene were found to contain a mixture of rHMf A molecules, ∼40% that retained the N-terminal formyl-methionyl residue (f-met-rHMfA), ∼50% that lacked ...
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