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  • Articles  (37)
  • Coleoptera  (37)
  • Springer  (37)
  • 1990-1994  (37)
  • 1991  (37)
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  • Articles  (37)
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  • Springer  (37)
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  • 1990-1994  (37)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 59 (1991), S. 75-78 
    ISSN: 1570-7458
    Keywords: Leptinotarsa decemlineata Say ; Coleoptera ; Chrysomelidae ; female sex pheromone ; screening method ; mating behaviour ; tactile stimuli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 2
    Electronic Resource
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    Entomologia experimentalis et applicata 61 (1991), S. 255-263 
    ISSN: 1570-7458
    Keywords: Coleoptera ; Bruchidae ; Bruchidius atrolineatus ; larval crowding ; post-embryonic development ; intracotyledonary mortality ; weight of emerging adults ; reproductive diapause
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Chez Bruchidius atrolineatus, l'accroissement de la densité larvaire à l'intérieur de la graine entraîne une augmentation de la mortalité alors que la réduction de poids des individus à l'émergence est relativement limitée. L'accroissement de la densité larvaire provoque une augmentation du taux d'adultes en diapause reproductrice, dans certaines conditions thermopériodiques. Dans d'autres conditions, permenttant l'émergence d'un fort taux d'adultes diapausants, l'effet de la densité larvaire est nul. Lorsque l'on analyse l'effet de la densité chez les individus d'une souche ne présentant pas de phénomène de diapause, il n'y a pas de corrélation entre la durée de développement et le poids des adultes. Par contre, lorsqu'il y a émergence d'adultes sexuellement actifs et diapausants, il existe une corrélation entre la durée de développement et le poids des adultes émergeants. Cette corrélation est due au fait que les adultes diapausants, qui présentent les durées de développement les plus longues, sont ceux qui ont les poids les plus faibles.
    Notes: Abstract In Bruschidius atrolineatus (Pic), an increase in larval density inside the seed led to a reduction in the survival rate during post-embryonic development, with only a limited decrease in the weight of adults. A high larval density increased the proportion of adults in reproductive diapause under certain thermoperiodic conditions. Under other conditions that already promote the emergence of a high proportion of diapausing beetles, an increase in larval density had no effect on diapause. There was no correlation between the larval developmental time and the weights of adults in a strain with a very low incidence of diapause. However, such a correlation was observed when both sexuallyactive and diapausing beetles emerged. This correlation was explained by the longer developmental times and lower weights of diapausing beetles.
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  • 3
    ISSN: 1423-0445
    Keywords: chemical defence ; mimicry ; reflex bleeding ; variation ; alkaloid ; coccinelline ; Coleoptera ; Coccinellidae ; Coccinella septempunctata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 7-spot ladybirds secrete alkaloid (coccinelline)-rich fluid (reflex blood) from leg joints as a defence mechanism against predators. A technique is described that enables the collection and accurate quantification of reflex blood produced, and the amount of coccinelline therein. Coccinelline was found distributed throughout the body, although concentrated in the reflex blood. Reflex blood was collected from a large set of beetles at several time points. Significant variation was found among beetles in the amount of reflex blood produced (for males and for females corrected for body weight) and the coccinelline concentration of the reflex blood. The results are discussed in relation to automimicry and the maintenance of variation through energy trade-offs. The relationships between tendency to aggregate, ability to reflex bleed and the possession of aposematic coloration are also considered.
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  • 4
    ISSN: 1423-0445
    Keywords: chemical defence ; mimicry ; reflex bleeding ; variation ; alkaloid ; adaline ; Coleoptera ; Coccinellidae ; Adalia bipunctata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 2-spot ladybirds secrete alkaloid (adaline)-rich defence fluid (reflex blood) in response to predator attack. Reflex fluid was collected from individual ladybirds and weighed and the alkaloid content measured by GC. The amount of fluid produced built up rapidly following winter hibernation in animals feeding on aphids. The concentration of adaline in the fluid was highest in the first bleeding after winter hibernation. A large sample of beetles was reflex bled several times. Significant among beetle variation was found in the amount of fluid produced and the concentration of the reflex blood. The results are discussed in relation to the possibility that 2-spot ladybirds are Batesian mimics of 7-spot ladybirds and to the possible functions of adaline.
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  • 5
    ISSN: 1423-0445
    Keywords: defensive secretion ; hot secretion ; elytral flanges ; evolution ; benzoquinones ; hydrocarbons ; bombardier beetle ; Coleoptera ; Carabidae ; Paussinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Australian bombardier beetle,Mystropomus regularis, sprays a mixture of quinones (1,4-benzoquinone, 2-methyl-1,4-benzoquinone, 2-ethyl-1,4-benzoquinone) and hydrocarbons (principallyn-pentadecane). The defensive fluid ist generated explosively in two-chambered glands, and is ejected audibly and hot (maximal recorded temperature = 59°C).Mystropomus is a member of the paussoid lineage of bombardiers. In common with other members of the group, it has a pair of elytral flanges (flanges of Coanda), associated with the gland openings, that serve as launching guides for anteriorly-aimed ejections of spray. It is argued thatMystropomus may be the least derived of flanged paussoids, and the closest living relative of the most primitive of extant bombardiers (Metriini).
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  • 6
    ISSN: 1423-0445
    Keywords: pyrrolizidine alkaloid N-oxides ; alkaloid sequestration ; defensive secretion ; host plant ; Coleoptera ; Chrysomelidae ; Oreina ; Asteraceae ; Adenostyles alliariae ; Senecio fuchsii ; Petasites paradoxus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Oreina cacaliae andO. speciosissima (Coleoptera, Chrysomelidae) sequester in their elytral and pronotal defensive secretions pyrrolizidine alkaloids (PAs) as Noxides (PA N-oxides). The PA N-oxide patterns found in the beetles and their host plants were evaluated qualitatively and quantitatively by capillary gas chromatography/mass spectrometry (GC-MS). Of the three host plantsAdenostyles alliariae (Asteraceae) is the exclusive source for PA N-oxide sequestration in the defensive secretions of the beetles. With the exception of O-acetylseneciphylline the N-oxides of all PAs ofA. alliariae, i.e. senecionine, seneciphylline, spartioidine, integerrimine, platyphylline and neoplatyphylline were identified in the secretion. PA N-oxides typical ofSenecio fuchsii (Asteraceae) were detected in the bodies of the beetles but not in their secretion. No PAs were found in the leaves of the third host plant,Petasites paradoxus (Asteraceae). The results suggest the existence of two distinctive storage compartments for PA N-oxides in the beetle: (1) the defensive secretion, containing specifically PA N-oxides acquired fromA. alliariae; (2) the body of the beetle, sequestering additionally but less selectively PA N-oxides from other sources,e.g. S. fuchsii or monocrotaline N-oxide fed in the laboratory. The concentration of PA N-oxides in the defensive secretion is in the range of 0.1 to 0.3 mol/1, which is more than 2.5 orders of magnitude higher than that found in the body of the beetle. No significant differences exist in the ability of the two species of beetles to sequester PA N-oxides fromA. alliariae, althoughO. speciosissima, but notO. cacaliae, produces autogenous cardenolides. A negative correlation seems to exist between the concentrations of plant-derived PA N-oxides andde novo synthesized cardenolides in the defensive secretion ofO. speciosissima.
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  • 7
    ISSN: 1423-0445
    Keywords: larval frass ; predator ; prey ; biological control ; kairomones ; Rhizophagus grandis ; Dendroctonus micans ; Coleoptera ; Rhizophagidae ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Adults of the predatory beetleRhizophagus grandis are strongly attracted to both adult and larval frass of its specific prey,Dendroctonus micans, in walking bioassays. Spruce bark and resin are relatively unattractive. Solvent extracts of larval frass that were attractive toR. grandis adults in a flight wind tunnel contained a mixture of monoterpenes of host plant origin. A synthetic mixture of these monoterpenes, (+)—α-pinene, (−)—β-pinene, β-phellandrene,dl limonene and 3-carene, was responsible for 70–80% of the activity of the most attractive extract. We suggest that a blend of monoterpenes in frass acts as a kairomone forR. grandis but that attraction to monoterpenes is only the first of a sequence of behavioural responses that ensures successful host location and identification.
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  • 8
    ISSN: 1573-1561
    Keywords: Aggregation pheromone ; Coleoptera ; Curculionidae ; Rhynchophorus palmarum ; American palm weevil ; (2E)-6-methy1-2-hepten-4-ol ; synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male American palm weevils (APWs),Rhynchophorus palmarum (L.) produced two sex-specific compounds, which were disclosed by volatile collections on Supelpak-2 and gas chromatography. One was a minor compound, not always detected. The major male-produced volatile was identified as (2E)-6-methyl-2-hepten-4-ol through coupled gas chromatography-mass spectrometry and gas chromatography-Fourier transform infrared spectrometry, proton nuclear magnetic resonance spectrometry, and rational synthesis. We propose the trivial name rhynchophorol for this new molecule, which proved to be the essential component of the APW aggregation pheromone by electroantennography, coupled gas chromatography-electroantennography and behavioral bioassays.
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  • 9
    ISSN: 1573-1561
    Keywords: Dendroctonus brevicomis ; Ips paraconfusus ; bark beetle ; verbenone ; ipsdienol ; pheromones ; inhibitors ; Coleoptera ; Scolytidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract DendroctonusBrevicomis andIps paraconfusus are sympatric bark beetle species colonizingPinus ponderosa in western North America. Interspecific and intraspecific competition for resources is, in part, mediated through semiochemicals. The response ofD. brevicomis to its attractant pheromone was significantly reduced by simultaneous release of either verbenone or racemic ipsdienol. Trap catch was significantly further reduced by including both inhibitors with the attractant pheromones. However, although the response ofIps paraconfusus was significantly inhibited with the addition of either verbenone or racemic ipsdienol, both compounds together did not result in a significant further reduction in trap catch. There was a trend for greater reduction in response ofD. brevicomis to attractant pheromones with increased release rates of either 69% (+)-/31% (−)-verbenone or 84% (−)-/16% (+)-verbenone. Response of associates to attractants and inhibitor combinations was also determined.
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  • 10
    ISSN: 1573-1561
    Keywords: Sitophilus oryzae ; Curculionidae ; Rhyzopertha dominica ; Bostrichidae ; Oryzaephilus surinamensis ; Cucujidae ; Tribolium castaneum ; Tenebrionidae ; Coleoptera ; essential oils ; fumigant toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The fumigant toxicity of 28 essential oils extracted from various spice and herb plants and some of their major constituents were assessed for adult coleopteransRhyzopertha dominica, Oryzaephilus surinamensis, Tribolium castaneum, andSitophilus oryzae. Three groups of active materials were distinguished: (1) The compounds terpinen 4-ol, 1,8-cineole, and the essential oils of three-lobed sage, sage, bay laurel, rosemary, and lavender were most active againstR. dominica; (2) The compounds linalool,α-terpineol, and carvacrol and the essential oils of oregano, basil, Syrian marjoram, and thyme were most active againstO. surinamensis; and (3) the compound 1,8-cineole and the essential oils anise and peppermint were active againstT. castaneum.
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  • 11
    ISSN: 1573-1561
    Keywords: Ips pini ; Thanasimus dubius ; Coleoptera ; Scolytidae ; Cleridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The bark beetleIps pini (Say) displays variation in its response to and production of enantiomeric blends of its pheromone ipsdienol. One of the principal predators ofIps pini isThanasimus dubius (F.), which uses ipsdienol as a kairomone for prey location. During 1988 and 1989, in Wisconsin and Michigan, the response of both species to a range of enantiomeric blends of ipsdienol was investigated. Blends tested had the following ratios of the (S)-(+) to (R)-(−) enantiomers: 3%∶97%, 25%∶75%, 50%∶50%, 75%∶25%, and 97%∶3%. Either75% (+) ∶ 25% (−) or 50% (+)∶ 50% (−) ipsdienol captured the mostIps pini in both years at both sites. The 25% (+)∶75% (−) blend also caught moreIps pini than the control during both years at both sites. All blends tested were attractive toThanasimus dubius in both years at both locations. Blend preferences of both species were variable and labile at both sites. Response patterns of both species in Wisconsin were different from those in Michigan each year. Furthermore, response patterns of both species to the ipsdienol blends changed from 1988 to 1989 at both locations. A genetic component to this variation would permit predator-prey coevolution, as well as the development of resistance byIps pini to management strategies based on mass-trapping with single blends.
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  • 12
    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Hypera postica ; alfalfa weevil ; Medicago rugosa ; Medicago sativa ; insect resistance ; (Z)-oxacyclotridec10-en-2-one ; cis-9-dodecen-12-olide ; (Z)-12-hydroxydodec-9-enoic acid lactone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Steam distillate prepared from leaves ofMedicago rugosa Desr. could inhibit feeding by adult alfalfa weevils (Hypera postica (Gyll.) on membrane filters, whereas an equivalent amount of steam distillate prepared usingM. sativa L. had no effect on weevil feeding. Earlier work established that a 12-carbon lactone, (Z)-oxacyclotridec-10-en-2-one, was responsible for the feeding deterrent properties of the steam distillate fromM. rugosa. The concentration of this volatile varied with plant age and leaf position. The maximum concentration in leaves ofM. rugosa (area basis) was estimated, on the basis of tests using an inert substrate, to be too low to cause a detectable inhibition of feeding. No correlation between lactone content and weevil feeding was detected when leaves from similar positions on plants of different ages were used in a multiple choice bioassay. Although small amounts (〈 12.5μg) of (Z)-oxacyclotridec-10-en-2-one can inhibit adult alfalfa weevil feeding on 13-mm-diameter membrane filters, because leaf concentrations (area basis) of this compound are lower than required to cause a detectable inhibition of feeding on the inert substrate, and because concentrations do not correlate with weevil feeding, it is unlikely that this lactone is responsible for resistance to adult alfalfa weevil feeding exhibited byM. rugosa.
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  • 13
    ISSN: 1573-1561
    Keywords: Ahasverus advena (Waltl) ; foreign grain beetle ; Coleoptera ; Cucujidae ; 1-octen-3-ol ; volatile attractant ; aggregation pheromone ; population density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Volatiles were captured on Porapak Q from foreign grain beetles,Ahasverus advena (Waltl), feeding on rolled oats at various population densities. At low population density, males, females, and mixed-sex beetles four to six weeks posteclosion and older produced 1-octen-3-ol. Mixed-sex beetles emitted almost pure (R)-(−) enantiomer. Weekly production rates of 1-octen-3-ol by males were at least four times greater than those of females. Production of 1-octen-3-ol was barely detectable in volatiles from mixed-sex adults maintained at the highest population density. Laboratory bioassays in a two-choice, pitfall olfactometer modified to retain responding beetles revealed that 1-octen-3-ol serves as an aggregation pheromone forA. advena. Both racemic and chiral 1-octen-3-ols were good attractants for mixed-sex adults in the pitfall olfactometer.
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  • 14
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    Journal of chemical ecology 17 (1991), S. 1517-1527 
    ISSN: 1573-1561
    Keywords: Pheromone ; ipsenol ; cis-verbenol ; chirality ; Ips latidens ; Coleoptera ; Scolytidae ; predator ; kairomone ; Enoclerus sphegeus ; Thanasimus undatulus ; Cleridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ipsenol was identified from the frass of male, but not female,Ips latidens from British Columbia, feeding in phloem tissue of lodgepole pine,Pinus contorta var.latifolia. The responses ofJ. latidens to sources of ips-enol andcis-verbenol were determined with multiple-funnel traps in stands of lodgepole pine in British Columbia. Ipsenol attracted both male and femaleI. latidens, verifying that it is a pheromone for this species. MaleI. latidens showed a slight preference for (S)-(−)-ipsenol.cis-Verbenol was not produced by beetles of either sex and, in contrast to an earlier report, both enantiomers inhibited attraction to ipsenol-baited traps. The predators,Enoclerus sphegeus andThanasimus undatulus (Cleridae), were attracted to traps baited withcis-verbenol and ipsenol.
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  • 15
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    Journal of chemical ecology 17 (1991), S. 2021-2034 
    ISSN: 1573-1561
    Keywords: Rapeseed ; Brassica spp. ; isothiocyanate ; thiocyanate ; defatted ; seed meal ; allelochemicals ; Limonius infuscatus ; wireworms ; Coleoptera ; Elateridae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A variety of plant pests are suppressed by the incorporation of cruciferous plant material into soil. Although this effect is attributed to decomposition of glucosinolates into toxic products, little is known concerning glucosinolate degradation in the soil environment. Arenas (30 × 18 × 8 cm) that contained soil amended with 30 g defatted winter rapeseed meal (Brassica napus L.)/kg soil on one half and unamended soil on the other were constructed. Isothiocyanate concentrations in the soil were measured using infrared analysis of CC14 extracts, and ionic thiocyanate (SCN−) using ion chromatography on aqueous extracts. Quantities were monitored during a 100-hr time period in conjunction with a wireworm bioassay. Isothiocyanate production reached a maximum of 301 nmol/g soil at 2 hr, but decreased by 90% within 24 hr. Production of SCN− reached a maximum of 180 nmol/g soil at 8 hr but persisted longer than isothiocyanate. Separate late instar wire-worms (Limonius infuscatus Mots.) were repelled by the presence of rapeseed meal in less than 24 hr even though the meal was shown in separate experiments not to be toxic. We propose that rapidly produced isothiocyanates are responsible for this repellency, but other products such as SCN− may play a role.
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  • 16
    ISSN: 1573-1561
    Keywords: Polygraphus rufipennis ; Coleoptera ; Scolytidae ; aggregation pheromone ; bark beetle ; black spruce ; 3-methyl-3-buten-1-ol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Porapak Q-captured volatiles of malePolygraphus rufipennis (Kirby) boring in black spruce and white spruce from Newfoundland and British Columbia, respectively, contained 3-methyl-3-buten-1-ol. Volatiles from logs in which the males had been joined by females contained the compound in reduced quantities. Hindgut extracts from male and femaleP. rufipennis disclosed no male-specific volatiles, but 3-methyl-3-buten-1-ol was detected in extracts of male-produced frass. The compound was not present in extracts from fresh phloem tissue.P. rufipennis of both sexes responded strongly in the field to traps baited with 3-methyl-3-buten-1-ol released at 4390 μg/day. There was little response to unbaited traps, fresh uninfested black spruce logs, or to 3-methyl-3-buten-1-ol released at lower rates. Combination of 3-methyl-3-buten-1-ol with either one of five terpenes prevalent in black spruce did not enhance beetle catch. Approximately half of 20 black spruce trees baited with 3-methyl-3-buten-1-ol were attacked, compared to 20.0% of 10 unbaited control trees. This new aggregation pheromone could be used to monitor or manageP. rufipennis populations.
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  • 17
    ISSN: 1573-1561
    Keywords: Dendroctonus micans ; Dendroctonus valens ; Rhizophagus grandis ; Coleoptera ; Rhizophagidae ; Scolytidae ; oviposition stimuli ; biological control ; monoterpenes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract During a laboratory study evaluatingRhizophagus grandis (a specific native predator of the Eurasian bark beetle,Dendroctonus micans), as a potential biocontrol agent against the North American bark beetle,Dendroctonus valens, it was found that feeding larvae and laboratory-produced frass of the potential prey elicited very high oviposition responses in the predator. Comparative chemical analysis of this laboratory-produced larval frass revealed that one major volatile compound, (-)-fenchone, is associated with the larvae of bothDendroctonus species.D. micans also generated pinocamphone while oxygenated monoterpenes in the frass ofD. valens were camphor,cis-4-thujanol, fenchol, terpinen-4-ol, myrtenal, pinocarvone, borneol, verbenone, piperitone, campholenaldehyde,trans-myrtanol,cis-myrtanol,p-cymen-8-ol and 5-oxo-camphor. This range of prey-produced compounds with a possible biological effect onR. grandis was narrowed down subsequent to comparative analysis of field-collected larval frass. (-)-Fenchone, pinocamphone, camphor, terpinen-4-ol, borneol, fenchol, and verbenone were found to be common to both prey species. A mixture of these seven components was tested in a bioassay, where it elicited as much oviposition as did larval frass ofD. micans. The oviposition stimulants forR. grandis are thus clearly among the mixture's constituents.
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  • 18
    ISSN: 1573-1561
    Keywords: Coleoptera ; Nitidulidae ; Carpophilus hemipterus ; dried fruit-beetle ; host-finding ; yeast ; wind tunnel ; attaction ; volatiles ; headspace
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The chemical basis underlying orientation to fruit and fungal odors was investigated for the dried-fruit beetle,Carpophilus hemipterus (L.). In wind-tunnel bioassays of walking and flight response from 1.8 m, beetles were attracted to odors of the yeastSaccharomyces cerevisiae on agar, aseptic banana, or banana inoculated withS. cerevisiae, although both banana substrates elicited greater response than the yeast alone. When presented in a two-choice bioassay, the yeast-inoculated banana attracted approximately twice as many beetles as did the aseptic banana. GC-MS analysis of the headspace volatiles above these odor sources revealed a somewhat more complex and concentrated volatile profile for yeast-inoculated banana than for aseptic banana. The odor from yeast on agar had fewer components, and these were present at lower concentrations than the odors of either banana substrate. By blending mineral-oil or aqueous solutions of the 18 components of inoculated-banana odor in varying concentrations, it was possible to mimic closely the headspace profile of the natural odor. This synthetic odor also elicited beetle attraction in the wind tunnel at levels comparable to the inoculated banana. Through a series of bioassays in which individual components were subtracted from or added to a synthetic odor blend, it was determined that ethyl acetate, acetaldehyde, 2-pentanol, and 3-methylbutanol comprised the simplest blend of compounds evoking full behavioral response. However, 2-methylpropanol or butanol were apparently interchangeable with 3-methylbutanol in this blend, and comparable response could also be elicited by replacing acetaldehyde with a combination of both 2-pentanone and 3-hydroxy-2-butanone. Thus, our results suggest that this generalist insect herbivore locates its hosts by a long-range response to a variety of blends of common fruit volatiles, whose concentrations are enhanced by fungi.
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  • 19
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    Journal of chemical ecology 17 (1991), S. 2323-2332 
    ISSN: 1573-1561
    Keywords: Galeruca tanaceti ; Coleoptera ; Chrysomelidae ; anthraquinones ; eggs ; larvae ; hemolymph ; ovaries ; Tanacetum vulgäre ; Achillea millefolium ; feeding deterrence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The overwintering eggs and the larvae of the leaf beetleGaleruca tanaceti (L.) contain hydroxylated anthraquinones. In both developmental stages, l,8-dihydroxy-3-methylanthraquinone (= chrysophanol) and 1,8-di-hydroxyanthraquinone (= chrysazin) were detected by GC-MS and GC-FTIR analyses. In the eggs, chrysazin was found only in traces. Anthraquinones were also present in ovaries and hemolymph of gravid females, which were investigated in order to examine the incorporation of these substances into the eggs. Neither in acidified nor in nonacidified extracts of the host plantsTanacetum vulgäre L. andAchillea millefolium L. were anthraquinones found. The activity of these anthraquinones as chemical defense substances was proved in bioassays with the antMyrmica ruginodis NYL. Further possible biological significances of anthraquinones are discussed.
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  • 20
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    Journal of chemical ecology 17 (1991), S. 989-1005 
    ISSN: 1573-1561
    Keywords: Host-plant resistance ; host-plant selection ; glycoalkaioids ; Leptinotarsa decemlineata ; Coleoptera ; Chrysomelidae ; Lycopersicon ; α-tomatine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The role of the steroidal glycoalkaloid α-tomatine in the hostplant resistance of tomato to the Colorado potato beetle,Leptinotarsa decemlineata (Say) was examined in short- (24 hr; using first- and fourth-instar larvae) and long-term (first-instar larvae reared through the prepupal stage) feeding experiments. Consumption rate, growth rate, efficiency of conversion of ingested food to body mass, and survival were compared forL. decemlineata provided foliage from susceptible (Lycopersicon esculetum Mill. cv. Walter), resistant (L. hirsutum f.Glabratum C.H. Mull accession PI 134417), and F1 hybrid plants. Values obtained for dietetic indices were regressed against corresponding values for α-tomatine content of foliage provided to larvae. Differences in dietetic indices could not be attributed to variation in foliar α-tomatine content despite a long-standing literature showing theex planta α-tomatine inhibits feeding and growth byL. decemlineata.
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  • 21
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    Journal of chemical ecology 17 (1991), S. 2469-2480 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Nitidulidae ; Glischrochilus quadrisignatus ; Glischrochilus fasciatus ; field traps ; volatile attractants ; host volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Seven volatile compounds identified from the headspace of whole wheat bread dough were investigated for their role in attractingGlischrochilus quadrisignatus andG. fasciatus in the field. Traps baited with either whole wheat bread dough or a synthetic seven-component bread dough odor caught similar numbers of these beetles, suggesting that the seven-compound combination could simulate the behavioral effect of bread dough. A series of trials using traps baited with various combinations of these chemicals showed that five compounds were significantly active in attractingG. quadrisignatus andG. fasciatus, but not all were essential for maximum response. The simplest blend eliciting a level of response comparable to the seven-component combination included ethyl acetate, acetaldehyde, ethanol, and racemic 2-methylbutanol, of which ethyl acetate, ethanol, and acetaldehyde were essential and 2-methylbutanol was replaceable with 2-methylpropanol forG. quadrisignatus attraction. Ethyl acetate and ethanol were essential for comparable attraction ofG. fasciatus. The chemical mediation of food finding in G.quadrisignatus andG. fasciatus is discussed in the context of volatile blends characterized for other nitidulid species.
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  • 22
    ISSN: 1573-1561
    Keywords: Dendroctonus frontalis ; Coleoptera ; Scolytidae ; Dinotiscus dendroctoni ; Hymenoptera ; Pteromalidae ; parasitoid ; kairomone ; electroan-tennogram
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    Notes: Abstract In two studies using the electroantennogram (EAG) technique, bark beetle- and tree-produced semiochemicals were presented toDinotiscus dendroctoni (Ashmead), a larval parasitoid ofDendroctonus frontalis Zimm. In the first study, 20 test compounds and a standard mixture of oxygenated monoterpenes were presented individually at one concentration to the parasitoids. In the second study, the nine compounds that elicited the greatest EAGs in study 1 were then tested as serial dilutions of 10 to 0.0001μg/ul. The individual compounds did not elicit responses greater than the standard mixture of oxygenated monoterpenes. Males and females exhibited similar dose responses, although females showed lower thresholds of response than males to frontalin, terpinen-4-ol,E,Z-chalcogran, andexo-brevicomin. In both studies, pino-/isopinocamphone elicited the greatest responses at high concentrations. Tests of different ratios of the camphone mixture indicated that pinocamphone elicited the greatest response. Most of the test compounds elicited similar responses which suggests that several of the compounds may be used together byD. dendroctoni in habitat and/or host community location.
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  • 23
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    Journal of chemical ecology 17 (1991), S. 285-308 
    ISSN: 1573-1561
    Keywords: Pheromone ; aggregation ; synergism ; ester ; carboxylic acid ; alcohol ; carboxylic ester ; driedfruit beetle ; Carpophilus hemipterus ; Coleoptera ; Nitidulidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The attractiveness of representative host materials, host extracts, and individual host volatiles (primarily carboxylic acids, alcohols, and esters) toCarpophilus hemipterus (L.) (Coleoptera: Nitidulidae) adults in wind-tunnel bioassays was examined. Attractiveness of the materials was examined alone and in combination with the aggregation pheromone. Host materials and extracts were often attractive on their own, and the attractancy was synergized when they were combined with the pheromone. Propanoic and butanoic acids, methanol, 2-propanol, 1-heptanol, methyl butanoate, and propanal were among the most effective attractants relative to the pheromone, but many other compounds significantly synergized the pheromone (typically three- to four fold). Attractiveness and synergism were influenced by the carbon chain length and branching of the substitutents. Straight-chain compounds that had at least three carbon atoms were generally effective as synergists. Many branched-chain compounds were also effective synergists. In general, the degree of attractiveness and synergism could be predicted fairly well with the physicochemical steric (Es) parameter, although the lipophilicity (Pi) parameter also appeared to be useful in explaining the lower activity of short-chain substituents. Thus, many compounds that had only limited attractiveness on their own may nevertheless play and important role in synergizing the pheromone. Structure-activity studies appear to be appropriate not only for determining optimal attractants for these insects, but also for determining effective synergists for the pheromone.
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  • 24
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    Journal of chemical ecology 17 (1991), S. 757-765 
    ISSN: 1573-1561
    Keywords: Monochamus alternatus Hope ; Coleoptera ; Cerambycidae ; male ; attractant ; (+)-cis-3-pinen-2-ol ; lightwood ; pine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A monoterpene alcohol was isolated from paraquat-induced lightwood in pines and identified as (+)-cis-3-pinen-2-ol [(+)-3]. More than a certain amount of (+)-3 induced a laboratory flight response by the male cerambycid beetle,Monochamus altematus Hope, but (+)-3 stimulated no response by the female at any dose level tested. Compound (+)-3 was not detected in trunks of sound pines.
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  • 25
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    Journal of chemical ecology 17 (1991), S. 805-809 
    ISSN: 1573-1561
    Keywords: 2-Methyl-1,4-benzoquinone ; m-cresol ; Tenebriomolitor ; Coleoptera ; Tenebrionidae ; defensive glands
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretion ofTenebrio molitor contains a mixture of 2-methyl-1,4-benzoquinone andm-cresol. The phenol had not previously been detected in the secretion, although some investigators reported presence of 2-ethyl-1,4-benzoquinone as a second component. We failed to detect the latter quinone in secretion samples from three laboratory populations ofT. molitor.
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  • 26
    ISSN: 1573-1561
    Keywords: Pheromone ; analog ; parapheromone ; behavior ; neurobiology ; olfaction ; single neuron ; electroantennogram ; boll weevil ; Anthonomus grandis ; Coleoptera ; Curculionidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Competitive field tests with α-fluorinated analogs of compounds III and IV (III-α-F and IV-α-F, respectively) of the boll weevil,Anthonomus grandis Boh., aggregation pheromone showed these compounds, when combined with the other pheromone components [(±)-I and II], to be as attractive as grandlure [(+)-I, II, and III+IV]. Dose-response curves constructed from electroantennograms of male boll weevils to serial stimulus loads of III, IV, III-α-F, IV-α-F, and the corresponding acyl fluorinated analogs (III-acyl-F and IV-acyl-F) showed the α-fiuorinated analogs to be as active as the pheromone components (threshold=0.1 μg), while the acyl fluorinated analogs had a 10-100 x higher threshold (=1-10 μg). Single-neuron recordings showed that IV neurons and II neurons (Dickens, 1990) responded to IV-α-F and III-α-F, respectively, while IV-acyl-F and III-acyl-F were inactive. Since a previous study showed compounds I, II, and IV to be essential for behavioral responses in the field, it seems likely that the activity of the α-fluorinated analogs observed here is due to the stimulation of IV neurons by IV-α-F as indicated in single neuron recordings.
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  • 27
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    Journal of chemical ecology 17 (1991), S. 1145-1158 
    ISSN: 1573-1561
    Keywords: Insecta ; Coleoptera ; Scolytidae ; Ips pini ; pheromone ; ipsdienol ; seasonal variation ; bioassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In May,Ips pini in New York did not respond in the field to 50–98.5% (R)-(−)-ipsdienol (synthetic). In September, beetles responded strongly to 50–60% (R)-(−)-ipsdienol (synthetic). In May and June, New York beetles showed marked preference for their own males over Arizona males, which produce an average of 94.1% (R)-(−)-ipsdienol. This suggested that ipsdienol stereochemistry alone does not ensure activity and that an additional compound is necessary for attraction in May. In the second year of field tests, attraction to synthetic ipsdienol and male beetles was tested in the spring, summer, and fall. There was response only to males in the spring and mid-summer and to both males and synthetic ipsdienol in the late summer and fall, causing a significant treatment x sampling period (date) interaction. Laboratory-reared beetles were not significantly more attracted to ipsdienol than to a blank airstream in laboratory assays, while male volatiles were significantly more attractive than ipsdienol and the blank. These data demonstrate that there is one or more unknown semiochemicals necessary for pheromonal response and that the behavioral activity of synthetic ipsdienol varies seasonally.
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  • 28
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    Journal of chemical ecology 17 (1991), S. 1557-1573 
    ISSN: 1573-1561
    Keywords: Laburnum anagyroides ; Robinia pseudacacia ; Aphis cytisorum ; Homoptera ; Aphididae ; Bruchidius villosus ; Coleoptera ; Bruchidae ; Lasius niger ; Formica species ; Hymenoptera ; Formicidae ; Triaspis thoracicus ; Braconidae ; Chalcidoidea ; quinolizidine alkaloids
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The quinolizidine alkaloids (QA) of golden rain,Laburnum anagyroides, and those of phytophagous insects associated with the plant, as well as of parasitoids of the latter, were analyzed by capillary GLC and GLC-MS. The alkaloid content in samples of vegetative plant parts was high at the beginning of the season, then decreased, while that of reproductive organs was high throughout flowering, pod formation, and maturation. The analyses showed that the QA of the plant passed through two higher trophic levels (herbivorous insects and their parasitoids) and that the alkaloid pattern changed little during the passage. The alkaloids were present in two phytophagous insect species associated with golden rain: the predispersal seed predator,Bruchidius villosus [5–13μg/g fresh weight (fw)], andAphis cytisorum (182–1012μg/g fw), an aphid that feeds on shoots, leaves, and inflorescences. Braconid and chalcidoid parasitoids emerging from the bruchid host also contained alkaloids (1.3–3μg/g fw), as did three foraging ant species,Lasius niger, Formica rufibarbis, andF. cunicularia (45μg/g fw), that visited the aphid colonies or honeydew-covered leaves of aphid-infested plants. The hypothesis that developing bruchid larvae and/or the plant “manipulate” QA supply to infested seeds was not supported, because QA content of leftover endosperm in seeds after bruchid development was similar to that of uninfested seeds. The frass of developing bruchid larvae was rich in QA (31μg/ g dry weight). While aphids sequestered, the bruchid larvae took up and eliminated QA with the frass without chemical transformation.
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  • 29
    ISSN: 1573-1561
    Keywords: Ips pini ; Thanasimus dubius ; Coleoptera ; Scolytidae ; Cleridae ; bark beetle ; ipsdienol ; aggregation pheromone ; kairomone ; coevolution ; mass-trapping ; intraspecific variation ; prey-predator interaction
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    Notes: Abstract The bark beetleIps pini (Say) displays variation in its response to and production of enantiomeric blends of its pheromone ipsdienol. One of the principal predators ofIps pini isThanasimus dubius (F.), which uses ipsdienol as a kairomone for prey location. During 1988 and 1989, in Wisconsin and Michigan, the response of both species to a range of enantiomeric blends of ipsdienol was investigated. Blends tested had the following ratios of the (S)-(+) to (R)-(−) enantiomers: 3%∶97%, 25%∶75%, 50%∶50%, 75%∶ 25%, and 97%∶ 3%. Either 75% (+)∶25% (−) or 50% (+)∶50% (−) ipsdienol captured the mostIps pini in both years at both sites. The 25% (+)∶75% (−) blend also caught moreIps pini than the control during both years at both sites. All blends tested were attractive toThanasimus dubius in both years at both locations. Blend preferences of both species were variable and labile at both sites. Response patterns of both species in Wisconsin were different from those in Michigan each year. Furthermore, response patterns of both species to the ipsdienol blends changed from 1988 to 1989 at both locations. A genetic component to this variation would permit predator-prey coevolution, as well as the development of resistance byIps pini to management strategies based on mass-trapping with single blends.
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  • 30
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    Journal of chemical ecology 17 (1991), S. 581-597 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Cucujidae ; grain beetles ; Oryzaephilus surinamensis (L.) ; Oryzaephilus mercator (Fauvel) ; Cryptolestes ferrugineus (Stephens) ; Ahasverus advena (Waltl) ; Cathartus quadricollis (Guér.) ; fungal volatiles ; attractant semiochemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses by five species of cucujid grain beetles (mixed-sex adults) to various volatiles were assessed by means of a two-choice, pitfall olfactometer. The test volatiles were short-chain alcohols and ketones known to be produced by fungi. Both racemic and chiral 1-octen-3-ols were strong attractants forCryptolestes ferrugineus (Stephens), as had been found previously forOryzaephilus surinamensis (L.),O. mercator (Fauvel), andAhasverus advena (Waltl). 3-Methylbutanol was another good attractant for these four cucujids, and it was the only test compound to whichCathartus quadricollis (Guér.) responded positively. 1-Octen-3-one, racemic 3-octanol, and 3-octanone showed various degrees of attractiveness for the former four species of cucujids.O. surinamensis was the only species of test beetle to show much positive response to 2-phenylethanol and ethanol. ForO. mercator andO. surinamensis, 3-methylbutanol enhanced positive response to their respective cucujolide aggregation pheromones.
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  • 31
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    Journal of chemical ecology 17 (1991), S. 647-661 
    ISSN: 1573-1561
    Keywords: Trypodendron lineatum ; Coleoptera ; Scolytidae ; flight behavior ; wind speed ; semiochemicals ; wind tunnel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The response of the striped ambrosia beetle,Trypodendron lineatum (Olivier) (Coleoptera: Scolytidae), to a semiochemical-baited funnel trap was studied in a wind tunnel in the first of two experiments. Wind speeds were tested over a range of 0.0–0.9 m/sec. Percent beetle capture decreased linearly with increasing wind speed within the range tested. A second experiment showed that in the presence of wind, beetles flew upwind to a semiochemical-baited substrate. In still air, they tended to fly randomly and erratically; yet in close proximity to the baited substrate, a greater proportion of the beetles were arrested in response to the chemical stimuli and landed on the substrate than when an airflow was present. These results suggest thatT. lineatum are capable of responding to semiochemicals under varied wind conditions typically present in a forest where they use wind to orient to olfactory stimuli. However, greater numbers are arrested in response to the stimuli under relatively still conditions.
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  • 32
    ISSN: 1573-1561
    Keywords: Storage pests ; aggregation pheromone ; Prostephanus truncatus ; larger grain borer ; Coleoptera ; Bostrichidae ; 1-methylethyl (2E 2-methyl-2-pentenoate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Male beetles of the larger grain borer,Prostephanus truncatus Horn (Coleoptera: Bostrichidae) were shown to produce material causing an electroantennographic (EAG) response from both male and female beetles. Volatiles from mixed sex cultures were collected on Porapak Q and fractionated by liquid chromatography (LC) with gradient elution. The total volatiles and the fractions were analyzed by gas chromatography (GC) linked to EAG, and two compounds eluting in the same LC fraction elicited EAG responses from male and female beetles. These were assumed to be components of the male-produced aggregation pheromone. Amounts of these compounds obtained were very low and less than 10−3 times the amounts of the aggregation pheromone components produced by the related species,Rhyzopertha dominica, under similar conditions. The most abundant of these EAG-active compounds was identified as 1-methyiethyl (2E)-2-methyl-2-pentenoate by comparison of its GC retention times and mass spectrum with those of synthetic analogs. The synthetic compound elicited electrophysiological and behavioral responses in both male and female beetles significantly greater than those to structural analogs. This compound is attractive toP. truncatus beetles in the field, and has been given the trivial name of “trunc-call 1”.
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  • 33
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    Journal of chemical ecology 17 (1991), S. 1159-1176 
    ISSN: 1573-1561
    Keywords: Insecta ; Coleoptera ; Scolytidae ; Ips pini ; pheromone ; isolation ; identification ; bioassay ; ipsdienol ; lanierone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A new pheromone component, lanierone, (2-hydroxy-4,4,6-trimethyl-2,5-cyclohexadien-1-one) was isolated and identified from a Porapak Q collection of volatiles from maleIps pini from New York through GC fractionation, bioassay, and spectrometry. In both the laboratory and the field, synthetic lanierone, in a 1:100 ratio with synthetic ipsdienol, is as attractive as natural pheromone sources. Synthetic ipsdienol alone is not attractive in the laboratory and only weakly attractive in the field. Varying the ratio of lanierone to ipsdienol in the field from 10−4∶1 to 1∶1 in 10-fold increments resulted in an increased number of beetles trapped at the three lower ratios, but also in an increase in the proportion of males trapped. In the field, all combinations of lanierone to ipsdienol attracted proportionately fewer males than did pheromone-producing male beetles. GC and GC-MS analyses of Porapak Q-trapped volatiles revealed that lanierone is produced in an amount equal to about 0.2% of that of ipsdienol and is produced exclusively by males. The small amount of lanierone produced, together with a GC retention time similar to that of ipsdienol on a nonpolar column, probably confounded its detection in earlier studies.
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  • 34
    ISSN: 1573-1561
    Keywords: Aggregation pheromone ; field trapping ; laboratory bioassay ; Coleoptera ; Curculionidae ; Rhynchophorus palmarum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field trapping of the American palm weevil (APW),Rhynchophorus palmarum, showed that the combination of caged male APWs and palm stem was much more attractive to APWs of both sexes than palm stem alone. Caged female APWs did not enhance the attractiveness of the palm. Caged APWs without palm stem were not attractive. Virgin laboratory-bred males were highly attractive to APWs of both sexes in a two-choice pitfall olfactometer, whereas virgin laboratory-bred females were not. Adsorbenttrapped volatiles from virgin laboratory-bred males reproduced the effect of living males, giving evidence for a male-produced aggregation pheromone in this species. Wild-mated APWs of both sexes were as responsive to the aggregation pheromone as virgin laboratory-bred APWs. This is the first record of chemical communication in this species. These results have prompted investigations into the chemical identification of the aggregation pheromone.
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  • 35
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    Journal of chemical ecology 17 (1991), S. 1273-1286 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Nitidulidae ; Carpophilus lugubris ; dusky sap beetle ; host finding ; chemical attractants ; wind-tunnel bioassay ; attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The chemical mediation of host-finding was investigated for the dusky sap beetle,Carpophilus lugubris Murray. GC-MS analysis of the headspace volatiles above whole-wheat bread dough inoculated with baker's yeast, a substrate previously determined to be an effective attractant, revealed seven major components in the following order of decreasing concentration: ethanol, acetaldehyde, 2-methylpropanol, 3-methylbutanol, propanol, 2-methylbutanol, and ethyl acetate. Solutions of these seven compounds blended so as to mimic the odor of whole-wheat bread dough elicited upwind orientation from 1.8 m in a wind tunnel byC. lugubris at a level comparable to that elicited by the bread dough. A series of bioassays investigating the role of individual components from the synthetic blend determined that all seven compounds contributed to behavioral activity; however, the simplest blend evoking attraction comparable to bread dough included acetaldehyde, ethyl acetate, 2-methylpropanol, and 3-methylbutanol. Of these compounds, acetaldehyde was essential, ethyl acetate was interchangeable with ethanol or partially replaceable with propanol, and 2-methylpropanol and 3-methylbutanol were partially replaceable with a combination of the other alcohols. Headspace volatiles above aseptic or fungus-inoculated tomato, banana, sweet corn, and strawberry were also qualitatively and quantitatively analyzed. This study suggests thatC. lugubris locates its food sources by response to variable blends of common volatile constituents of plants and fungi.
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  • 36
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    Journal of chemical ecology 17 (1991), S. 1421-1435 
    ISSN: 1573-1561
    Keywords: Interspecific mating ; Ips confusus ; Ips paraconfusus ; Coleoptera ; Scolytidae ; reproduction ; reproductive isolation
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Host and conspecific discrimination were tested as reproductive isolating mechanisms betweenIps paraconfusus Lanier infestingPinus coulteri (Torrey) andI. confusus (Le Conte) infestingP. monophylla (Fremont). In two areas (one area largely Coulter pine and the other largely pinyon pine) where these bark beetles and hosts cooccur in southern California, we induced pheromone production in host and nonhost logs using males of each species.Ips paraconfusus females joined both heterospecific and conspecific males tunneling in both tree species in each area.Ips confusus females failed to joinI. paraconfusus males in Coulter pine, but joinedI. paraconfusus in pinyon pine. Sympatry was demonstrated when females of both sibling species joined conspecific males in their respective hosts. Males attacked all four beetlehost treatment combinations in both areas. Laboratory tests confirmed these results. Males did not displace heterospecific males from nuptial chambers in hosts, and they did not occupy a gallery in which heterospecific males produced frass. Females that left conspecific males in a host were readily accepted by heterospecific males and oviposited in a nonhost.
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  • 37
    ISSN: 1573-1561
    Keywords: Sinaibin ; glucosinolate ; resistance ; Sinapis alba ; Brassica napus ; Phyllotreta cruciferae ; Coleoptera ; Chrysomelidae ; Mamestra configurata ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Sinalbin was identified as a chemical component of insect anti-xenosis and antibiosis resistance mechanisms in seedlings ofSinapis alba by DEAE-Sephadex chromatography, HPLC, treatment with sulfatase and myrosinase, various feeding tests using artificial and natural substrates, and by measuring sinalbin concentrations in cotyledons and leaves during seedling development. The effects of sinaibin on feeding were dependent upon the insect species and upon the rapidly changing profile of sinaibin concentrations in the developing seedling. The high concentrations of sinalbin found in young cotyledons (up to 20 mM) and leaves (up to 10 mM) deterred the feeding of the flea beetle,Phyllotreta cruciferae Goeze and larvae of the bertha armyworm,Mamestra configurata Walker. The protection that sinalbin confers upon the vulnerable, newly emerged seedling (and upon tiny, young leaves) appears critical for the first few days of survival ofS. alba under feeding pressure from flea beetles in the field. The lower concentrations of sinaibin found in older cotyledons and leaves (2–3 mM) offer little or no protection againstP. cruciferae and may actually stimulate the feeding of this crucifer specialist. These concentrations of sinaibin, however, are still effective in reducing the level of feeding by larvae of the more generalist feederM. configurata.
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