Publication Date:
1991-01-25
Description:
Fractionation of transcription extracts has led to the identification of multiple transcription factors specific for each form of nuclear RNA polymerase. Accurate transcription in vitro of the yeast U6 RNA gene by RNA polymerase C requires at least two factors. One of them was physically and functionally indistinguishable from transcription factor IID (TFIID or BTF1), a pivotal component of polymerase B transcription complexes, which binds to the TATA element. Purified yeast TFIID (yIID) or bacterial extracts that contained recombinant yIID were equally competent to direct specific transcription of the U6 gene by RNA polymerase C. The results suggest the formation of a hybrid transcription machinery, which may imply an evolutionary relation between class B and class C transcription factors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Margottin, F -- Dujardin, G -- Gerard, M -- Egly, J M -- Huet, J -- Sentenac, A -- New York, N.Y. -- Science. 1991 Jan 25;251(4992):424-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departement de Biologie, Centre d'Etudes Nucleaires de Saclay, Gif-sur-Yvette, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1989075" target="_blank"〉PubMed〈/a〉
Keywords:
Electrophoresis, Polyacrylamide Gel
;
HeLa Cells
;
Humans
;
Nuclear Proteins
;
RNA Polymerase III/*metabolism
;
RNA, Fungal/biosynthesis/genetics
;
RNA, Small Nuclear/*genetics
;
Saccharomyces cerevisiae/*genetics
;
TATA Box/*genetics
;
Templates, Genetic
;
Transcription Factor TFIID
;
Transcription Factor TFIIIB
;
Transcription Factors/*genetics
;
*Transcription Factors, General
;
*Transcription, Genetic
;
*Transcriptional Elongation Factors
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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