ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • tissue culture  (34)
  • Springer  (34)
  • Annual Reviews
  • Blackwell Publishing Ltd
  • 2005-2009
  • 1990-1994  (34)
  • 1980-1984
  • 1990  (34)
Collection
Keywords
Publisher
Years
  • 2005-2009
  • 1990-1994  (34)
  • 1980-1984
Year
  • 1
    Electronic Resource
    Electronic Resource
    Plant regeneration from leaf and seed-derived calli and suspension cultures of the African perennial wild rice, Oryza longistaminata (1990)
    Springer
    Plant cell reports 9 (1990), S. 447-450 
    Details
    ISSN: 1432-203X
    Keywords: African wild rice ; Oryza longistaminata plant regeneration ; tissue culture ; cell suspension ; histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Plant regeneration from 2-month-old callus cultures derived from immature leaves of 7-day-old aseptic seedlings and mature embryos of the African wild rice Oryza longistaminata was achieved at 20% and 100% frequency, respectively. The morphogenic potential of the embryo-derived calluses dropped from 100% at the third subculture to 12.5 % at the 12th subculture. Five-month-old morphogenic calluses were used to establish a fast-growing suspension culture which, when plated onto semisolid medium, still retained its ability to regenerate plantlets 9 months after initiation. Histological analyses demonstrated that late plant regeneration from established callus and suspension cultures occured through organogenesis, although some embryogenesis events may have taken place during initiation of these cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232270
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Plant regeneration from a cryopreserved embryogenic cell suspension of a commercial sugarcane hybrid (Saccharum sp.) (1990)
    Springer
    Plant cell reports 9 (1990), S. 419-423 
    Details
    ISSN: 1432-203X
    Keywords: Cell suspension ; cryopreservation, Gramineae ; Saccharum ; sugarcane ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Efficient plant regeneration was obtained from a cryopreserved embryogenic cell suspension of sugarcane established from leaf derived callus. Pregrowing the cells for three days in MS basal medium supplemented with 0.33 M sorbitol was essential to the process. The cells were cooled at a rate of 0.5°C/min to −40°C and then stored in liquid nitrogen. Thawing was carried out rapidly in water at +40°C, and the cells were then plated without washing onto filter paper discs placed on a semi-solid regeneration medium (MS basal + 3% sucrose + 0.13 mg/1 2,4-D +0.25 mg/1 BAP + 0.25 mg/1 kinetin + 0.25 mg/1 zeatin). The filter paper discs, along with the cells, were transferred to the same, fresh medium after five hours. After 24 hours the cells were scraped off, placed on fresh semi-solid medium and incubated at 28°C in the dark for two weeks before transfer to light. A regeneration efficiency of 92% was obtained (regenerated plants, expressed as a percent of unfrozen control). Plants regenerated from cryopreserved cells, and grown to maturity in the greenhouse, were morphologically identical to regenerated control plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232263
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Morphogenetic effect of glycerol on tissue cultures of the red seaweed Grateloupia doryphora (1990)
    Springer
    Journal of applied phycology 2 (1990), S. 137-143 
    Details
    ISSN: 1573-5176
    Keywords: carbon-source ; glycerol ; Grateloupia doryphora ; morphogenesis ; osmolality ; solidity ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants of Grateloupia doryphora were cultivated in Provasoli Enriched Seawater culture medium (PES) supplemented with glycerol (0.1, 0.3, 0.5 or 0.8 mol 1−1) or carbohydrates (0.1 or 0.3 mol 1−1 mannose, glucose and galactose) and agar (3, 8, 15 g 1−1 ). The osmolality of the medium was adjusted by dilution of the seawater (70 or 100%, v/v). The increase in fresh weight of explants cultivated in liquid medium with glycerol (0.3 mol 1−1) and without glycerol was compared. All experiments were carried out in the light, except for one assay in which the explants were cultivated in the dark. Glycerol was an effective carbon source for the vegetative propagation of G. doryphora in solid and liquid media. Mannose, glucose and galactose all had no effect on growth or morphogenesis of the explants. In solid media the main effect of glycerol was as a morphogenetic inductor, with PES70 (70% seawater) + 0.1 or 0.3 mol 1−1 glycerol + 3 or 8 g 1−1 agar the best formulation. An increase in the concentration of agar in glycerol-containing medium reduced the morphogenetic capacity of the explants, which developed into compact cell masses. The effects of glycerol were observed only in explants cultivated under light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023375
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Comparison of the isozyme variation in tall fescue parents and their somaclones (1990)
    Springer
    Euphytica 51 (1990), S. 249-256 
    Details
    ISSN: 1573-5060
    Keywords: Festuca arundinacea ; tall fescue ; chromosome pairing ; electrophoresis ; isoenzyme ; meiotic analyses ; somaclonal variation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was conducted using the isozymes ACP-1, ADH-1, GOT-2, GOT-3, MDH, 6-PGD-1 and PGI-2 to: a) compare isozyme banding patterns of tall fescue somaclones with parents and b) correlate tissue culture-induced chromosome abnormalities with variant banding patterns. The 174 somaclones were grouped into seven categories based on their meiotic analyses and time of regeneration from culture. Differences in isozyme frequency between categories compared by chi-square tests were greatest for MDH, 6-PGD-1 and PGI-2, and least for ACP-1. The most significant differences in frequency were found between somaclones and parents. In comparisons of somaclone categories, the most different isozyme distributions were between the early vs. late regenerated somaclones. No significant differences in isozyme frequencies were found between all 42-chromosome somaclones vs. aneuploid somaclones and the three somaclone groups (42-normal, 42-abnormal, aneuploid) compared to each other. This study suggests that culture-induced isozyme variation alters the distribution of the isozyme phenotypes, but is not directly correlated with chromosome abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039726
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Somaclonal variation in Lotus corniculatus L. in relation to plant breeding purposes (1990)
    Springer
    Euphytica 46 (1990), S. 35-41 
    Details
    ISSN: 1573-5060
    Keywords: forage legumes ; Lotus corniculatus ; birdsfoot trefoil ; somaclonal variation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seventy-two plants regenerated from leaf-derived calli of a single plant of Lotus corniculatus have been evaluated for several morphological and agronomical traits. The analysis of selfed and polycross progenies of the regenerants indicates that the variation among regenerants was, at least in part, of genetic origin. Most of the mutations induced by tissue culture were recessive and were detected only after sexual propagation. Although in vitro culture had a depressive effect for most of the traits, the selfed progenies of 2 regenerants displayed higher values for leaflet width and seed yield than the selfed progeny of the initial plant. However the somaclonal variation did not increase the variation for any trait with respect to the variation of the donor cultivar of the initial plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00057616
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    In vitro Separation of Chimeral Pears into their Component Genotypes (1990)
    Springer
    Euphytica 48 (1990), S. 189-196 
    Details
    ISSN: 1573-5060
    Keywords: tissue culture ; Pyrus ; chimera ; anthocyanins ; segregation ; mutant ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An adventitious shoot regeneration system for Pyrus communis was used to separate two chimeral pears into their component genotypes. The two cultivars were a variegated type, ‘Louise Bonne Panachée’ and a red fruited mutant, ‘Red Hardy’. Leaves of these cultivars were placed onto a regeneration medium consisting of Nitsch & Nitsch (1969) salts supplied with various levels of Thidiazuron (TDZ) and NAA. After two months the regenerants were moved onto a proliferation medium of Lepoivre salts. Later they were evaluated for their chimeral status. Among the regenerants of the variegated type, 100% segregation occurred, most shoots were green, a few were albino. Regeneration was more efficient for dissociating the variegated chimera than rapid shoot multiplication and physical injury. In ‘Red Hardy’, after two months on the regeneration medium, 20 to 33% of the regenerants were green, the rest were red. The stability of the red and the green regenerants were assessed on media supplemented with various levels of sucrose and by total anthocyanins measurement. Both types were stable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037199
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Genetic control of somatic embryogenesis in cotton petiole callus cultures (1990)
    Springer
    Euphytica 49 (1990), S. 249-253 
    Details
    ISSN: 1573-5060
    Keywords: Gossypium hirsutum ; cotton ; somatic embryogenesis ; callus ; tissue culture ; genetic control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three commercial varieties (Acala SJ-5, Coker 312 and Paymaster 303) and three exotic accessions (T1, T25 and T169) of cotton (Gossypium hirsutum L.) were tested for ability to undergo somatic embryogenesis. Sections of split petiole were cultured on 3 media and evaluated for embryogenesis after 180 days. Embryogenic T25 and Coker 312 plants were selected and crossed in a diallel with non-embryogenic Acala SJ-5, Paymaster 303, T1 and T169 plants. F1, F2 and BC1 populations were generated and tested for embryogenesis on a medium of MS salts and vitamins (1962) plus (per liter) 4.0 mg NAA, 1.0 mg Kn, 30 g glucose, 100 mg myo-inositol, 2.0 g Gelrite and 0.75 g MgCl2. Segregation for both occurrence and magnitude of embryogenesis was observed, suggesting the action of more than one gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036296
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in cotyledons of Picea abies is enhanced by an adventitious bud-inducing treatment (1990)
    Springer
    New forests 4 (1990), S. 125-135 
    Details
    ISSN: 1573-5095
    Keywords: Norway spruce ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Somatic embryos were obtained from young, isolated cotyledons of Picea abies. A bud-inductive treatment for one week before transfer to a callus induction medium, resulted in a tenfold increase in the percentage of cotyledons in which embryonic tissues could be induced. The degree of expression of somatic embryogeny depends on whether cotyledons are first subjected to a callus-inducing medium or first exposed to a cytokinin treatment followed by a callus-inducing medium. It is hypothesized that two populations of cells exist, both with the inherent potential of becoming embryogenic after treatment with plant growth regulators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00119005
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    The effects of the physical characteristics of the culture medium on the development of red seaweeds in tissue culture (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 137-142 
    Details
    ISSN: 1573-5117
    Keywords: callus ; Gelidium versicolor ; Grateloupia doryphora ; Laurencia sp. ; osmolality ; regeneration ; solidity ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants of Gelidium versicolor, Grateloupia doryphora and Laurencia sp. were cultivated in Provasoli enriched seawater culture medium (PES) adjusted to several osmolalities (0.5, 0.7, 1.0 and 1.5 Os kg−1) and solidities (agar concentration = 3, 8 and 15 g L−1). Osmolality was adjusted by dilution of seawater with distilled water (50, 70 and 100% seawater) and by NaCl addition. Explants of Laurencia sp. and Grateloupia doryphora showed bud regeneration and callus formation. Explants of Gelidium versicolor only showed bud regeneration. Osmolalities of 0.5 and 1.05 Os kg−1. inhibited or drastically reduced bud regeneration and callus formation. The highest callus formation and bud regeneration were observed at 0.7 to 1.0 Os kg−1. An increase in the agar concentration of the culture medium was positively correlated with callus formation and negatively correlated with bud regeneration. An increase in the percentage of seawater increased the solidity of the culture medium and was positively correlated with callus formation. Glycerol was an effective carbon source for the vegetative propagation of axenic explants of Grateloupia doryphora, promoting growth and bud regeneration. An increase in glycerol concentration in the culture medium increased its osmolality, inhibiting the growth of the explants and their morphogenetic development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040225
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Some effects of plant growth regulators on tissue cultures of the marine red alga Agardhiella subulata (Gigartinales, Rhodophyta) (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 353-360 
    Details
    ISSN: 1573-5117
    Keywords: Agardhiella subulata ; plant growth regulator ; seaweed ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined whether auxins and cytokinins, either singly or in combination, stimulate cell division in tissue cultures of a red seaweed. Our experimental model consisted of filamentous and callus-like growths that developed from cross-sectional discs cut from young branches of Agardhiella subulata. Plant growth regulators were added to the medium to give combinations of an auxin with a cytokinin over a range of concentrations (1 µg L−1 −10 mg L−1). Several mixtures of auxins and cytokinins, as well as some single auxins, cytokinins and phenolics, stimulated cell division and growth in the tissue cultures beyond that of controls. The treatments that were effective included: phenylacetic acid/zeatin; phenylacetic acid/6-benzylaminopurine; α-naphthaleneacetic acid/zeatin; 2,4,5-trichlorophenoxyacetic acid/6-benzylaminopurine; and indoleacetic acid/kinetin. High concentrations of cytokinins (i.e. 10 mg L−1) inhibited the regeneration of plants in some of the cell cultures. These results provide further evidence that growth regulators can be used for the tissue culture of seaweeds and for the study of developmental phenomena in these plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040256
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 223-228 
    Details
    ISSN: 1573-5044
    Keywords: Rubus ; thidiazuron ; kanamycin ; tissue culture ; cytokinins ; leaf organogenesis ; cotyledon organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thidiazuron (TDZ), a substituted urea with cytokinin activity, was significantly more effective than benzyladenine (BA) at inducing organogenesis from detached Rubus cotyledons and leaves. The optimal TDZ concentration for organogenesis from cotyledons and leaves was 5–10 µM and 5–20 µM, respectively; however, shoot organogenesis occurred at TDZ concentrations of 0.5–5.0 µM. Light fluence rates from 0–81 µmol m-2 s-1 of continuous fluorescent light did not affect the percentage of organogenesis from cotyledons. Two weeks of continuous dark did not enhance the percentage of regeneration from leaf explants. Kanamycin, an antibiotic used to screen for transformation, severely reduced cotyledon growth and regeneration at concentrations as low as 10 mgl-1, and completely inhibited shoot organogenesis at 50 mgl-1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041885
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Micropropagation of juvenile and adult Quercus suber L. (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 1-8 
    Details
    ISSN: 1573-5044
    Keywords: cork oak ; micropropagation ; Quercus suber ; tissue culture ; rooting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes research on the application of tissue culture techniques to the micropropagation of cork oak (Quercus suber L.), a forest species of ecological and industrial importance in the Mediterranean area. Apical buds and nodal stem segments were employed as initial explants. Their origins were young seedlings, stump sprouts and sprouts formed on cuttings collected from old trees. The action of the mineral medium and growth regulators was studied in the multiplication stage. Media with low concentrations of ions, such as Sommer's or Heller's, are more suitable for growth and proliferation of explants than other media richer in salts. It was also observed that cytokinin (BA) must be present for the culture development. Adding low concentrations of auxin (NAA) to the medium improves the multiplication rate, especially in vegetative material of adult origin. The auxin type is the most important factor in the promotion of rhizogenesis. The method of application determines the quality of the root system. Treatment with low concentrations of IBA added to the rooting medium gives the best results. High sucrose concentration also improves rooting. Diluting the mineral rooting medium is slightly favourable, although there is no significant difference between it and the standard mineral concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034484
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Organogenesis and plant regeneration from immature embryos of Rosa hybrida L. (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 147-152 
    Details
    ISSN: 1573-5044
    Keywords: adventitious shoots ; histology ; Rosaceae ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Intact, flowering, rose plants have been regenerated in vitro from excised embryos of crosses between ‘Bridal Pink’ (the maternal parent) and several pollen parents. Explanted embryonic tissues developed into an organogenic callus which formed adventitious shoots after several months only on a modified half-strength Murashige & Skoog medium containing 1.0 μM BA and 0.05 μM NAA. These shoots could be separated, grown individually, rooted in a medium with no BA or NAA, with 1.0 μM IBA, and transplanted to greenhouse media. Embryos ranging in age from 21 to 35 days post-pollination formed organogenic callus that eventually regenerated adventitious shoots. Histological examination of normally-developing embryos showed that well-defined embryonic axes were beginning to develop at approximately 20–25 days postpollination. Analysis of populations of regenerated plants from different crosses showed differences in flower color, growth habit, peduncle length, and petal number. This system may be useful for irradiation-mutation breeding and/or for the development of transgenic rose plants using Agrobacterium tumefaciens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033434
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    The effect of some macronutrients on adventitious root development on scion apple cultivars in vitro (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 185-189 
    Details
    ISSN: 1573-5044
    Keywords: ammonium nitrate ; Malus ; nitrogen ; potassium nitrate ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of some macronutrients, especially NH4NO3 and KNO3, on root development of microcuttings from 3 apple scion cultivars is discussed. A reduction of the level of NH4NO3 in the medium from full strength to 1/4 strength significantly increased the percentage rooting of ‘Gala’ and ‘Royal Gala’, but not ‘Jonagold’. Further reduction of NH4NO3 level from 1/4 strength to zero significantly reduced the percentage of rooting in ‘Gala’ but not ‘Royal Gala’. ‘Jonagold’ rooted best at zero concentration NH4NO3. Without NH4NO3, rooting percentages were as high as 100% for all 3 cultivars when KNO3 was provided at full strength. The results show that adventitious roots can be induced on apple scion cultivars by media manipulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033440
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Callus production, regeneration and evaluation of plants from cultured inflorescences of tall fescue (Festuca arundinacea Schreb.) (1990)
    Springer
    Plant cell, tissue and organ culture 22 (1990), S. 7-15 
    Details
    ISSN: 1573-5044
    Keywords: somaclone ; tissue culture ; meiosis ; isozyme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty genotypes (individual plants) of ‘Kentucky 31’ tall fescue (Festuca arundinacea Schreb., 2n=6x=42) were evaluated to determine regeneration response, and meiotic and isozyme changes in the regenerants. Six genotypes (K8, K16, K25, K27, P3 and P13) were selected for study of efficiency in producing calli and regenerating plants. Panicle pieces (3569) were plated on Schenk & Hildebrandt medium with one of three auxins, 2,4-D (2.0 mg 1-1), pCPA (3.8 mg 1-1) or 2,4,5-T (2.5 mg 1-1). Square-root transformed data were analysed as a completely randomized 6×3 factorial with genotype and auxin as the main effects. F-protected means were compared by the Waller-Duncan K-ratio T test. Genotype K8 produced significantly more calli per panicle piece (88/877), whereas genotype K25 regenerated significantly more plantlets per panicle piece (58/214) than the other genotypes. Callus production was significantly higher using 2,4-D (102/1244 calli per piece) than pCPA or 2,4,5-T. The type of auxin did not have a statistically significant effect on plant regeneration, which suggested that genotype was the most important variable. Twelve of the 210 regenerants were albino. Cytological evaluation of 95 green regenerants showed that all plants had 21 bivalents and pollen stainability ranged from 43.0–98.4%, which suggested all plants were male fertile. Zymograms of these 95 regenerants for ACPH, ADH, GOT, MDH, 6-PGD, PGI and SOD showed no differences from that of the parental genotype, which suggested that the green plantlets regenerated from somatic tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043692
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Culture conditions effecting plant regeneration from cotyledons of Vigna radiata (L.) Wilczek (1990)
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 1-7 
    Details
    ISSN: 1573-5044
    Keywords: cotyledon culture ; genotypic differences ; plant growth-regulators ; plant regeneration ; tissue culture ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conditions for plant regeneration from excised cotyledons of Vigna radiata were studied. Complete plant developed from the uncallused proximal ends of cotyledons on Murashige & Skoog's (MS), Gamborg's (B5) and ‘C’ (MS salts + B5 vitamins) basal media. The basal medium ‘C’ was found to be best for plant regeneration. Regeneration frequency, however, varied with genotype, size, orientation and age of explant and the different plant growth regulators combination in the medium. Addition of cytokinins induced callusing at the proximal ends of cotyledons followed by multiple shoot formation. Out of 6-benzyl aminopurine (BAP), kinetin (KIN), N (Δ−2 isopentyl) adenine (2iP) and adenine sulphate (AS), only BAP and KIN were found to be more effective in enhancing the frequency of shoot regeneration. BAP at 1×10-1M induced maximum (60%) shoot regeneration whereas maximum number of shoots (8 to 9 shoots) per explant was observed with 5×10-6M BAP. Cotyledons excised from two-day old seedlings were most regenerative. The regenerative response of cotyledons decreased when sliced into two equal parts either longitudinally or transversely. Callusing and organogenic differentiation occured only if the petiolar end of cotyledons was in contact with medium. None of the tested treatments were effective in inducing shoot bud differentiation from subcultured callus. Well developed shoots rooted when incubated on half strength MS, MS and MS basal medium supplemented with IAA (5×10-6M). The rooted plants were transferred to pots and later established in the field with 60% success.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00116082
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Relationship between calcium and agar on vitrification and shoot-tip necrosis of quince (Cydonia oblonga Mill.) shoots in vitro (1990)
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 135-142 
    Details
    ISSN: 1573-5044
    Keywords: agar ; calcium ; cydonia ; mineral nutrition ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot-tip cultures of Quince C (Cydonia oblonga Mill.) initiated on Murashige & Skoog (MS) medium containing 5 μM BA and 0.6% Phytagar showed both shoot-tip necrosis and severe vitrification. Culturing explants on medium containing 1.2% Phytagar and Ca levels of 3 mM (MS medium), 18 mM and 30 mM showed a decrease in growth with increasing medium Ca levels, being especially severe at 30 mM. The Ca content of the explants increased linearly with increasing medium Ca. Culturing explants on medium containing 3 mM, 9 mM, and 18 mM Ca at 0.6, 0.9, and 1.2% agar resulted in reduction in growth, shoot-tip necrosis, and vitrification when either factor was increased. The reduction in shoot-tip necrosis could be accounted for primarily by an increase in medium Ca levels but may also be affected by a change in explant growth. Increasing Ca concentration in the medium resulted in a linear increase in explant K, Ca, Mg, and B levels and a decrease in Mn and Na. Although increasing medium Ca or agar levels reduced vitrification, it is unclear whether they were the direct cause of the reduction in vitrification or whether this response was an effect of the reduction in culture fresh weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035834
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis in two Gossypium hirsutum genotypes on semi-solid versus liquid proliferation media (1990)
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 201-204 
    Details
    ISSN: 1573-5044
    Keywords: gelling agent ; suspension culture ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparison of semi-solid vs. liquid embryo proliferation media was made using two Gossypium hirsutum L. genotypes (Coker 312 and T25) and two callus initiation media. Sections of petioles from mature, flowering plants were cultured on two modified Murashige and Skoog media. Medium 1 included 4.0 mg l-1 NAA and 1.0 mg l-1 kinetin; medium 2 contained 0.1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. After six weeks, callus was removed from each explant and divided in half. One callus portion was placed in liquid proliferation medium and the other on semi-solid (0.2% Gelrite) proliferation medium. Composition of proliferation medium was identical to that of initiation medium, except no growth regulators were added. Embryos were counted after eight weeks. The percentage of explants forming callus was influenced by genotype/initiation medium combination. Analysis of variance procedures revealed significant variability for callus initiation media, proliferation media (semi-solid or liquid), and an initiation medium x genotype interaction. Paired t-tests indicated that more embryos were produced in liquid proliferation medium (227.3 embryos/culture) than on semi-solid proliferation medium (134.6 embryos/culture).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034432
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Clonal micropropagation of mature Larix (1990)
    Springer
    New forests 4 (1990), S. 63-66 
    Details
    ISSN: 1573-5095
    Keywords: European larch ; in vitro ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Adventitious buds were developed in vitro from stems of elongating short shoots of mature European larch (Larix decidua Mill.). Tissue culture propagule populations thus generated, were similarly remultiplied in vitro. Elongation and rooting were routine. No vitreous or otherwise abnormal shoots were noted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00119591
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    Micropropagation of field tested superior Eucalyptus grandis hybrids (1990)
    Springer
    New forests 4 (1990), S. 67-79 
    Details
    ISSN: 1573-5095
    Keywords: elongation ; initiation ; rooting ; sprouts ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three superior clones of Eucalyptus grandis hybrids were micropropagated through several steps. Five-year-old trees were girdled to induce juvenile sprouts. Cultures were attempted from mature branches and sprouts. Branches from mature trees were 100% contaminated while sprouts were only 40% contaminated. Pre-initiation hormone free medium and dark environment were used to screen for contaminants and to reduce production of phenolic compounds. Initiation of auxillary buds was achieved with modified MS plus 0.05 mg/l NAA and 0.5 mg/l BAR High multiplication rates were obtained on auxin-free medium with 0.6 mg/1 BAR Elongation of shoots was best on media with high auxin (2.5 mg/l of IBA) and cytokinin (1–1.5 mg/l of zeatin). Continual subculture on the multiplication medium improved rooting significantly. Up to 98% rooting was achieved on 1/4 MS with 2 mg/l IBA. Rooted propagules were successfully transferred to a mist greenhouse with 82% survival, and then to greenhouse conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00119001
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Callus formation in Ecklonia cava Kjellman (Laminariales, Phaeophyta) (1990)
    Springer
    Hydrobiologia 204-205 (1990), S. 375-380 
    Details
    ISSN: 1573-5117
    Keywords: callus ; Ecklonia cava ; Laminariales ; Phaeophyta ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants from stipes and meristems of Ecklonia cava were incubated on six media under several culture conditions. Both stipe and meristem explants developed calluses three to six weeks after inoculation onto all media except AS PC-1. Calluses developed on stipe explants but did not develop on meristem explants at a temperature of 23 °C. Temperatures from 8 to 13 °C were favorable for callus development. Callus development on meristem explants required light but callus development on stipe explants did not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040259
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Studies on in vitro selection of Fe-efficient lines in sugarcane (1990)
    Springer
    Plant and soil 129 (1990), S. 183-186 
    Details
    ISSN: 1573-5036
    Keywords: chlorosis ; Fe-efficiency ; sugarcane ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Iron deficiency in sugarcane is common on many calcareous soils of South India. The local cane cultivar Co 740 is found to be Fe-inefficient. During the present investigation callus-derived Fe-efficient lines were obtained through in vitro selectrion on Fe-stress MS medium. The Fe deficient basic medium was created by adding 100 mg CaCO3 and 0.5 mg Fe L-1 as FeEDTA in nutrient solution, and the pH was adjusted to 8.5 to impose stress conditions. The selected callus was used for producing shoot buds. The green shoots were further used for regeneration of roots. These selected seedlings were green, and grew continuously on the Fe-stress medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032411
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis from pea embryos and shoot apices (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 7-14 
    Details
    ISSN: 1573-5044
    Keywords: genotype ; plant growth regulators ; Pisum sativum ; plant regeneration ; somatic embryogenesis ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Conditions were defined for plant regeneration via somatic embryogenesis in pea, using explants from immature zygotic embryos or from shoot apices. For the induction of somatic embryos, an auxin (picloram or 2,4-dichlorophenoxyacetic acid) was required. Embryogenic callus originated from embryonic axis tissue of immature embryos and from the axillary-bud region and the plumula of shoot apices. A clear effect of embryo size on somatic embryogenesis was shown. There were differences in frequency of somatic embryogenesis among the five genotypes used in the study. Additions of BA to auxin-containing medium reduced embryo production. Histological examinations confirmed the embryogenic nature of the immature embryo cultures and revealed that somatic embryos originated from the meristematic areas near the callus surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034751
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    In vitro clonal propagation of dioecious Carica papaya (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 41-46 
    Details
    ISSN: 1573-5044
    Keywords: papaya ; endophytic contamination ; rifampicin ; micropropagation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for in vitro propagation of dioecious papaya clones is described. A high rate of success in culture estbalishment was obtained when axillary buds were taken from lateral shoots of hedged rooted cuttings grown in a greenhouse. Seasonal endophytic contamination was suppressed by shaking propagules for 24 h in 300 mgl-1 rifampicin or by incorporating it at 50 mgl-1 into the medium. Murashige & Skoog (MS) basal medium supplemented with 0.5 mgl-1 6-benzyladenine and 0.1 mgl-1 naphthaleneacetic acid was used for establishment and proliferation. The addition of 160 mgl-1 adenine sulfate improved multiplication and shoot growth. An elongation stage on MS medium supplemented with 1.0 mgl-1 kinetin and 0.05 mgl-1 naphthaleneacetic acid was necessary before rooting. Rooting was obtained at a high rate on half-strength macroelements of MS medium supplemented with 1.0 mgl-1 indole-3-butyric acid. Commercial plots of papaya plants obtained through this procedure already exist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034755
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev (syn. Chrysanthemum morifolium Ramat.) (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 21-30 
    Details
    ISSN: 1573-5044
    Keywords: chrysanthemum ; tissue culture ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adventitious shoots were regenerated from leaf and stem explants of eleven chrysanthemum cultivars. The optimum medium for both explant types contained Murashige & Skoog basal medium supplemented with 5 μM 6-benzylaminopurine and 5 μM α-napthaleneacetic acid. Generally, stem explants were superior to leaf explants. There were large cultivar differences in shoot regeneration frequency with three cultivars failing to respond over a wide range of hormone combinations. Shoots on stem explants appeared mainly to originate from cortical cells which rapidly divided and ruptured the epidermis. Regenerated shoots could be easily rooted, transferred to glasshouse conditions, and grown to flowering. All regenerated plants had the same morphological characteristics compared to plants derived from nodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034487
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    The development of dormancy in bulblets of Lilium speciosum generated in vitro (1990)
    Springer
    Plant cell, tissue and organ culture 22 (1990), S. 167-172 
    Details
    ISSN: 1573-5044
    Keywords: bulbs ; dormancy ; lily ; micropropagation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied the effect of various in-vitro conditions on dormancy of bulblets generated on scale explants of Lilium speciosum Thunb. cv. ‘Rubrum’ nr. 10. The bulblets were harvested after 11 weeks of culture. Dormancy was measured by determining the percent emergence in soil of viable, non-cold-treated bulblets. A study of the physical conditions showed that temperature had a strong effect on the induction of dormancy (15°C induced hardly any dormancy; 25°C induced a high level of dormancy), whereas short or long day and light or dark had no effect. Of the medium components, a low concentration of sucrose (1 gl−1 or less) or a high concentration of gibberellic acid (1 mg 1−1) reduced the level of dormancy. Application of various concentrations of abscisic acid, 6-benzylaminopurine, α-naphthaleneacetic acid, indole-3-acetic acid, 2,3,5-triiodobenzoic acid or a Murashige and Skoog macro- and microelement mixture did not affect the dormancy status.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033631
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Influence of cultural and physiochemical factors on ascorbate stability in plant tissue culture media (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 131-135 
    Details
    ISSN: 1573-5044
    Keywords: L-ascorbic aid ; dehydroascorbic acid ; tissue culture ; medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ascorbic acid rapidly decays in plant tissue culture media. Within 50 min to 3 h after preparing 100 mM solutions, ascorbic acid was destroyed. Autoclaving, shaking flasks, high light intensity and increasing pH over a range from 4.5–7 accelerated decay. Ascorbic acid was oxidized to dehydroascorbic acid which also underwent decay. Within 11 h and 15 min after adding ascorbic acid both ascorbic acid and its oxidation product, dehydroascorbic acid, disappeared from medium. Since ascorbic acid is rapidly destroyed in plant tissue culture media it may not exert its effect as an intact molecule. Instead its antioxidant/antibrowning role in plant cell, tissue and organ cultures may be mediated by some product of further oxidation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00114711
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.) (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 137-140 
    Details
    ISSN: 1573-5044
    Keywords: Citrus unshiu ; embryogenesis ; juice vesicle ; plantlet formation ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus was induced from juice vesicles of satsuma mandarin on Murashige & Skoog medium supplemented with α-naphthaleneacetic acid (NAA), kinetin (K) and gibberellin (GA). Adventitious embryoids arose from the callus tissue on the medium containing 1 mgl−1 NAA alone. The embryoids grew into embryos which resulted in a plantlet on medium containing 1 mgl−1 GA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00114712
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Comparison of 2,4-D and picloram for selection of long-term totipotent green callus cultures of sugarcane (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 157-163 
    Details
    ISSN: 1573-5044
    Keywords: auxin ; 2,4-D ; long-term regeneration ; picloram ; Saccharum ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Long-term regeneration of sugarcane (Saccharum spp. hybrid and Saccharum spontaneum L.) callus cultures was achieved by selection of green callus on MS agar medium containing 0.5 mgl-1 picloram or 2,4-D. Newly initiated sugarcane callus cultures were a complex mixture of different tissue types including white, nonregenerative and green, regenerative tissues. The proportion of the tissue types changed as a function of time in culture, genotype, and amount and kind of auxin. Green callus on picloram media always regenerated green plants. Nine hybrids and ten wild relatives of sugarcane produced green calli on picloram media whereas only three hybrids were grown as green calli on 2,4-D media in long-term culture. Green calli were inoculated into liquid MS medium with 0.5 mgl-1 picloram for suspension culture. These cultures were totipotent after 19 months. For routine culture, we initiated callus cultures on modified MS medium with 3 mgl-1 2,4-D, then in two to three weeks we subcultured callus on MS medium with 0.5 mgl-1 picloram and selected for green callus. Green calli regenerated large numbers of green plants after more than four years.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041876
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Assessment of the changes of 2,4-dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal (1990)
    Springer
    Plant cell, tissue and organ culture 20 (1990), S. 165-172 
    Details
    ISSN: 1573-5044
    Keywords: activated charcoal ; 2,4-D adsorption ; coconut palm ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rate of adsorption of 2,4-dichlorophenoxyacetic acid (2,4-D) by activated charcoal (AC) from liquid and semi-solid tissue culture media was determined using 2-[14C]-2′,4′-D. In liquid medium 99.5% of the added 2,4-D (10-4 M) was adsorbed by AC (2.5 gl-1) within 5 days of preparation of the medium. Higher 2,4-D levels of reduced AC concentrations increased the level of available 2,4-D in the medium and extended the period necessary for the level of 2,4-D in the medium to become stabilized. In semi-solid medium the rate of adsorption of 2,4-D by AC was considerably reduced. A stable ratio of gel/2,4-D:AC/2,4-D was only reached after 10 to 20 days, depending on the 2,4-D concentration used. Low pH levels and maintenance of the medium at higher temperatures (20–30°C) accelerated the adsorption of 2,4-D by AC. In vitro tissue cultures of coconut palm showed marked differences in their growth response according to the age of the medium used and the associated variations in 2,4-D concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041877
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 79-82 
    Details
    ISSN: 1573-5044
    Keywords: Drosera capensis ; Drosera natalensis ; plumbagin ; Plumbago auriculata ; secondary metabolites ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rapid clonal multiplication of two species of South African Drosera is described. Levels of plumbagin, (5-hydroxy-2-methyl-1,4-naphthoquinone) from in vivo and in vitro grown plants are compared to those present in Plumbago roots. P. auriculata Lam. roots contained more than twice as much plumbagin as in vivo grown D. capensis L. plants which in turn contained more than twice as much as comparable plants of D. natalensis Diels. It is concluded that the extraction of plumbagin from Drosera plants is not commercially feasible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034496
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    The influence of acclimatization treatment and plantlet morphology on early greenhouse-performance of tissue-cultured Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] (1990)
    Springer
    Plant cell, tissue and organ culture 21 (1990), S. 111-117 
    Details
    ISSN: 1573-5044
    Keywords: tissue culture ; acclimatization ; plantlet quality ; adventitious rooting ; Douglas fir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this investigation was to test for the possible effects of plantlet morphology and environmental stress on survival and growth during the acclimatization of tissue-cultured Douglas fir [Pseudotsuga menziesii (Mirb.) Franco]. Under a high-stress environmental regime of 40–70% relative humidity and 22–28°C, survival was 33%, compared to 89% under a low-stress regime of 80–90% RH and 15–20°C. Shoot elongation under low stress was twice as great as that under high stress. Certain morphological features were associated with improved survival under high stress, and they included a large (10+) root number, tall (40 + mm) shoots, and upright needles. Plantlets with 10 + roots had 52% survival, compared to 25% or 31% for those with 1–3 or 4–10 roots, respectively. Also, plantlets with an initial shoot height of 41–60 mm had a 53% survival rate compared to 20% or 37% for those with 21–30 mm or 31–40 mm shoots. Tall plantlets and those with 10+ roots also underwent the greatest shoot elongation during the 7 week observation period. However, plagiotropism was frequent on tall shoots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033429
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Induction of haploids in Populus maximowiczii via embryogenic callus (1990)
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 49-58 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; embryoids ; poplars ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Anthers of Populus maximowiczii with microspores at the mononucleate stage were cultured at 20°C in the dark on agar-solidified Murashige and Skoog medium after 4 days of cold treatment (4°C). After 4 to 8 weeks anthers on medium supplemented with 0.5, 1.0 or 2.0 mg l-1 2,4-D in combination with 0.1 mg l-1 kinetin developed calli that were characterized by smooth surface and gel-like consistency. These calli were comprised of expanding microspores surrounded by a mucilaginous matrix. After transfer of anthers with embryogenic calli to MS medium with low hormone levels (NAA at 0, 0.1 and 0.1 mg l-1 and BA at 0, 0.1 and 1.0 mg l-1) microspores started to divide and initiated independent meristematic nests, which developed into embryoidal structures, resembling globular to bi-polar heart-shaped embryoids. The embryoids germinated precociously without developing cotyledons. After transfer to medium with a range of levels of BA (1.0, 2.5 and 5.0 mg l-1), adventitious shoots developed mainly from the roots. Shoots were rooted in half strength MS medium supplemented with 0.025 mg l-1 NAA. Via this pathway anther response in the best treatment combination was 10%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00116089
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Allelopathy of small everlasting (Antennaria microphylla) (1990)
    Springer
    Journal of chemical ecology 16 (1990), S. 931-939 
    Details
    ISSN: 1573-1561
    Keywords: Allelopathy ; Antennaria microphylla ; small everlasting ; Euphorbia esula ; leafy spurge ; tissue culture ; hydroquinone ; arbutin ; glucosyltransferase ; biotransformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Media and media extracts from callus cultures of small everlasting (Antennaria microphylla) inhibited leafy spurge (Euphorbia esula L.) callus tissue and suspension culture growth (50 and 70% of control, respectively) and were phytotoxic in lettuce and leafy spurge root elongation bioassays (64 and 77% of control, respectively). Hydroquinone, a phytotoxic compound previously isolated from small everlasting, was also biosynthesized by callus and suspension cultures of this species. Exogenously supplied hydroquinone (0.5 mM) was toxic to leafy spurge suspension culture cells and was only partially biotransformed to its nontoxic water-soluble monoglucoside, arbutin, by these cells. This report confirms the chronic involvement of hydroquinone in the allelopathic interaction between small everlasting and leafy spurge.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01016501
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...