ISSN:
1573-4927
Keywords:
Drosophila
;
sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
;
glue proteins
;
glycosylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The patterns of protein fractions from total salivary glands and from glue plugs were compared in seven members of theDrosophila nasuta subgroup by the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The glue protein patterns are member specific concerning the numbers and the electrophoretic mobilities of major and minor glue protein fractions. However, the major fractions of all subgroup members could be grouped into five SDS-PAGE domains according to the homologies of their electrophoretic mobilities, prominence of Coomassie blue staining, and PAS reaction. In all subgroup members, major fractions are involved in posttranslational modifications into larger protein molecules of the final glue. Quantitative estimations of the glue proteins inD. n. nasuta andD. n. albomicans reveal that they constitute between 55 and 60% of the total salivary gland proteins, whereas inD. melanogaster and inD. hydei the fraction is only 32 and 35%, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00553628
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