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  • tissue culture  (32)
  • Springer  (32)
  • Inst. f. Geophys., Ruhr-Univ. Bochum
  • Saint Louis University
  • Springer Nature
  • 1985-1989  (32)
  • 1988  (32)
Collection
Keywords
Publisher
  • Springer  (32)
  • Inst. f. Geophys., Ruhr-Univ. Bochum
  • Saint Louis University
  • Springer Nature
  • Wiley-Blackwell  (2)
Years
  • 1985-1989  (32)
Year
  • 1
    Electronic Resource
    Electronic Resource
    A new device to open tissue culture flasks (1988)
    Springer
    Methods in cell science 11 (1988), S. 31-33 
    Details
    ISSN: 1573-0603
    Keywords: tissue culture ; flask opening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A device has been designed to open tissue culture flasks, which is quick and permits easy access for cell cloning, the removal of complete cell sheets, and staining for microscopy. The brass blade used melts the plastic rather than burning, thus reducing the level of potentially toxic fumes produced by some other methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404412
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  • 2
    Electronic Resource
    Electronic Resource
    Ferulate esters of higher fatty alcohols and allelopathy inKalanchöe daigremontiana (1988)
    Springer
    Journal of chemical ecology 14 (1988), S. 589-603 
    Details
    ISSN: 1573-1561
    Keywords: Kalanchöe daigremontiana ; triacontanol ; ferulate esters ; ferulic acid ; tissue culture ; allelopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ferulate esters of normal C22–C30 alcohols were found in the root extract ofKalanchöe daigremontiana and the freen-C30 alcohol, triacontanol, was found on the leaves. Ferulic acid was isolated from the vermiculite in which plants were grown. Whole plant and tissue culture experiments were done to investigate the role of ferulic acid as an allelochemical and of triacontanol as a plant growth regulator inK. daigremontiana and other bioassay systems. No positive growth responses to triacontanol were observed, but inhibitation of growth response of plantlets by ferulic acid was seen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01013909
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  • 3
    Electronic Resource
    Electronic Resource
    Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants (1988)
    Springer
    Plant molecular biology 11 (1988), S. 139-145 
    Details
    ISSN: 1573-5028
    Keywords: Lycopersicon esculentum Mill. ; ribosomal DNA ; Solanum lycopersicoides Dun. ; somatic hybrid ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment polymorphisms were used to identify and quantify the nuclear contributions from each parent to somatic hybrid plants between tomato (Lycopersicon esculentum Mill.) cv. Sub-Arctic Maxi and Solanum lycopersicoides Dun. Three single-copy clones, 2–13, 2–17, and 3–288, and a clone for the 45s ribosomal RNA, pHA2, all mapped to chromosome 2 of tomato, were used in analysis of 47 somatic hybrids. The amount of hybridizing probe for each parental band was quantified by densitometry of the autoradiograph film. Analyses with the three single-copy clones indicated that there were more than two S. lycopersicoides copies in most somatic hybrid plants. For at least one somatic hybrid there was a loss of one tomato copy. No evidence was found for more than two copies donated from tomato or loss of a copy from S. lycopersicoides. Most of the observed variation in copy number of the single-copy clones was consistent with chromosomal changes occurring in the suspension cells from which S. lycopersicoides parental protoplasts were derived. The number of copies of rDNA derived from each parent varied independently of the number of copies of single-copy clones from each parent. Changes in the copy number of rDNA occurred in both tomato and S. lycopersicoides genomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015666
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  • 4
    Electronic Resource
    Electronic Resource
    Media for cultivation of animal cells: an overview (1988)
    Springer
    Cytotechnology 1 (1988), S. 199-214 
    Details
    ISSN: 1573-0778
    Keywords: animal cells ; growth factors ; mammalian cells ; media ; serum ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The increasing interest in products from animal cells has caused an extensive research effort towards development of media for cell cultivation. The basic components in the media used for cultivation of animal cells vary depending upon the characters of the cells and the cultivation method. Basic components consist of an energy source, nitrogen source, vitamins, fats and fatty soluble components, inorganic salts, nucleic acid precursors, antibiotics, oxygen, pH buffering systems, hormones, growth factors and serum. Extensive efforts are directed towards developing serum-free or chemically defined media. Among the serum substitutes is a long list of hormones and growth factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00145023
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  • 5
    Electronic Resource
    Electronic Resource
    Preparation of human embryonic liver cultures (1988)
    Springer
    Bulletin of experimental biology and medicine 105 (1988), S. 248-250 
    Details
    ISSN: 1573-8221
    Keywords: human embryo ; liver ; hepatocytes ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00835708
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  • 6
    Electronic Resource
    Electronic Resource
    Morphological characteristics of human and experimental animal leproma cell cultures and effect of antileprosy drugs on them (1988)
    Springer
    Bulletin of experimental biology and medicine 106 (1988), S. 1781-1785 
    Details
    ISSN: 1573-8221
    Keywords: leprosy ; tissue culture ; morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00802196
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of carbamylcholine on membrane potential and Na-K pump activity of cultured rat skeletal myotubes (1988)
    Springer
    Cellular and molecular neurobiology 8 (1988), S. 393-410 
    Details
    ISSN: 1573-6830
    Keywords: acetylcholine (ACh) receptor ; desensitization ; membrane potential ; skeletal muscle ; tissue culture ; Na-K pump ; carbamylcholine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary 1. We measured changes in resting membrane potential (E m) and Na-K pump activity, assayed by ouabain-sensitive86Rb uptake, in response to carbamycholine (CCh) and its continued presence in single rat skeletal myotubes in culture. 2. CCh caused immediate depolarization from controlE m (-80 to -85 mV) to near 0 followed by repolarization of varying degrees depending on the age of the culture and temperature of the recording medium; repolarization ofE m was most apparent by culture age 8–9 daysin vitro (DIV),E m reaching values as high as -60 mV by 5–10 min after peak depolarization at 37°C. 3. Input resistance, which decreased during CCh depolarization, increased only slightly during the initial phase of repolarization and then remained essentially unchanged during the major component of membrane repolarization in the presence of CCh. 4. Ouabain, given before CCh, prevented repolarization ofE m and, when given after repolarization had begun, reversed it and causedE m to return to about -7 mV. 5. Na-K pump activity was decreased in myotubes in whichE m did not repolarize or did so only slightly, and was increased by over 40–50% in myotubes whoseE m repolarized by 40–60 mV, even though CCh was still present in the medium. Inhibition of pump activity in non repolarizing myotubes was related to Na influx, inhibition being reversed to stimulation when CCh was administered to myotubes in Na-free medium. 6. Repeated (three or four times) or prolonged (up to 60-min) administration of CCh to myotubes in which repolarization was hardly expressed (age 6–7 DIV) caused increases both in the amount of repolarization and in86Rb uptake, both being related to the number or duration of CCh exposures. 7. We conclude that repolarization ofE m following CCh-induced depolarization of cultured rat skeletal myotubes depends to a large extent on an increase in activity of the electrogenic Na-K pump.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00711225
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  • 8
    Electronic Resource
    Electronic Resource
    Hybridization in Brassica juncea × Brassica campestris through ovary culture (1988)
    Springer
    Euphytica 37 (1988), S. 83-88 
    Details
    ISSN: 1573-5060
    Keywords: Brassica ; oilseed crops ; tissue culture ; interspecific hybridization ; biotechnology ; genetic variability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ovary culture has been employed for the production of interspecific hybrids of a partially compatible cross of Brassica juncea (2n=36) × Brassica campestris (2n=20). Five to seven days old ovaries cultured on White's medium supplemented with casein hydrolysate (300 mg/l) and sucrose (5%) produced more seeds than any other media tried, but seed development was better on media fortified with plant hormones. The seed yield was better in B. juncea × B. campestris than their reciprocal cross. The plants obtained from ovary-derived seeds were transferred to the field; they were intermediate in some morphological characters and chromosome number (2n=28) as compared to their parents. The flower buds generally did not open and had poorly developed anthers with mostly sterile pollen. The pod size/setting was very much reduced, but healthy seeds were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037228
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  • 9
    Electronic Resource
    Electronic Resource
    Effects of auxin concentration on induction and growth of embryogenic callus from young inflorescence explants of Old World bluestem (Bothriochloa spp.) and bermuda (Cynodon spp.) grasses (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 13-19 
    Details
    ISSN: 1573-5044
    Keywords: Bothriochloa ischaemum ; Cynodon dactylon ; forage grasses ; auxin ; tissue culture ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Objectives of this research were to test the effects of plant genotypes and auxin 2,4-D (2,4-dichlorophenoxyacetic acid) medium concentrations on embryogenic (E) callus production of two grass species. Two Old World bluestem,Bothriochloa ischaemum, accessions (A-8793 and A-8911c) and three bermudagrass,Cynodon dactylon (L.) Pers., accessions (A-10978b, A12164, and ‘Brazos’) supplied the explant material. Immature inflorescences ≤9 mm in length were placed on modified Murashige-Skoog (MS) agar medium containing 0, 1, 3, or 5 mg L-1 of 2,4-D. Explants of all genotypes produced callus by the end of a 4-week dark incubation period at 25°C. When subcultured onto fresh media and maintained at 25°C with a 16 hr photoperiod, calli became embryogenic within 8 weeks of inoculation. Three mg L-1 of 2,4-D in the media maximized E callus production in both bluestem genotypes and in A-10978b and A-12164 bermudagrass genotypes. Maximum E callus production from Brazos bermudagrass resulted from the 1 mg L-1 treatment. Somatic embryos developed after subculture under light. Embryos showed scutellum-like structures and coleoptile-coleorhiza bipolar organization. Plantlets were regenerated from all genotypes except Brazos, whose embryoids failed to germinate. All callus from Brazos eventually senesced. Light and scanning electron microscopy confirmed regeneration through somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043103
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  • 10
    Electronic Resource
    Electronic Resource
    The production of strawberry plants from callus cultures (1988)
    Springer
    Plant cell, tissue and organ culture 12 (1988), S. 235-241 
    Details
    ISSN: 1573-5044
    Keywords: tissue culture ; callus ; organogenesis ; strawberry ; somacloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoots were regenerated from callus of the commercially important strawberry varieties Bogota, Brighton, Cambridge Favourite, Hapil, Ostara, Rapella, Red Gauntlet and JILA33 which is a promising selection from a current breeding programme. The callus was initiated from explants of petiole or lamina of leaves of micropropagated shoots in vitro or of lamina or peduncle from greenhouse plants. There was more shoot regeneration with callus from lamina than from petiole although with the variety Hapil, regeneration occurred only with callus from peduncle. With seven of the varieties, shoot regeneration occurred on culture media with BAP and 2,4-D whilst with the remaining variety, Cambridge Favourite, it occurred only with medium which contained 1AA-β alanine conjugate in place of 2,4-D. Regenerated shoots rooted readily and the plants produced are being studied for somaclonal variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00034364
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