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  • Articles  (125)
  • Wiley-Blackwell  (125)
  • American Meteorological Society
  • 1985-1989  (125)
  • 1920-1924
  • 1988  (125)
  • Natural Sciences in General  (125)
Collection
  • Articles  (125)
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Keywords
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Years
  • 1985-1989  (125)
  • 1920-1924
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Fungal infections in the acquired immunodeficiency syndrome (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 115-131 
    Details
    ISSN: 0741-0581
    Keywords: Electron microscopy ; Fungal diagnosis ; Fungal therapy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Defects in cell-mediated immunity caused by infection with the human immunodeficiency virus (HIV) render AIDS patients particularly susceptible to fungal pathogens. Signs and symptoms of serious infection may be nonspecific, and early diagnosis and institution of antifungal therapy is essential to decrease morbidity and mortality in this patient population. In a symptomatic individual, invasive procedures are often required to establish a microbiologic diagnosis, and histopathologic examination of tissue by light and electron microscopy is often the first indication of a serious fungal infection in an AIDS patient.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080108
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  • 2
    Electronic Resource
    Electronic Resource
    EELS log-ratio technique for specimen-thickness measurement in the TEM (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 193-200 
    Details
    ISSN: 0741-0581
    Keywords: Thickness measurement ; Electron energy-loss spectroscopy (EELS) ; Inelastic mean free path ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: We discuss measurement of the local thickness t of a transmission microscope specimen from the log-ratio formula t = λ In (It/I0) where It and I0 are the total and zero-loss areas under the electron-energy loss spectrum. We have measured the total inelastic mean free path λ in 11 materials of varying atomic number Z and have parameterized the results in the form λ = 106F (E0/Em)/ln (2βE0/Em) where F = (1 + E0/1,022)/(1 + E0/511)2, the incident energy E0 is in keV, the spectrum collection semiangle β is in mrad, and Em = 7.6Z0.36. This formulation should allow absolute thickness to be determined to an accuracy of ±20% in most inorganic specimens.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080206
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  • 3
    Electronic Resource
    Electronic Resource
    Evaluation of high-resolution electron microscopy as a method for studying Y-Ba-Cu-O superconductors (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 273-284 
    Details
    ISSN: 0741-0581
    Keywords: Superconductors ; Electron microscopy ; Perovskites ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: High-resolution transmission electron microscopes operating at 300 and 400 kV were used to investigate the crystallography and microstructure of the perovskitelike YBa2Cu3O7-x. In this paper, we evaluate the performance attainable with these microscopes both empirically and by computer modelling. Based upon the assumption that oxygen may be a key to superconductivity properties, we have also investigated the visibility of the oxygen sites as well as the heavier yttrium and barium ion positions and the lighter Cu atom positions. We propose a scheme for observing different twin orientations in these structures and hence the oxygen atom positions seen in projection for the [100] and [010].Our observations of both thick and thin regions of Y-Ba-Cu-O materials are reported as well as the problems of adjusting microscope parameters and specimen alignment to obtain interpretable images. We also give a preliminary report on the effects of heat treatment as seen in high-resolution micrographs to assess disorder of the heavy atoms and oxygen vacancies.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080306
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural investigation of renal biopsies: A discussion of artifacts and special methodology (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 9 (1988), S. 283-291 
    Details
    ISSN: 0741-0581
    Keywords: Ultrastructure ; Kidney ; Artifacts ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The aim of this presentation is to draw attention to the problems inherent in evaluating the ultrastructure of percutaneous renal biopsies and to discuss some of the special techniques which are useful in this area. It is important to realize that the ultrastructure as it appears in this kind of material does not necessarily reflect conditions in vivo. Comparison with suitable reference material may, however, permit reliable conclusions in terms of pathological diagnosis and pathogenesis. It is advocated that purely qualitative methods, which until now have predominated in ultrastructure work with renal biopsies, be replaced by morphometry and semiquantitative methods when it is possible and practical to do so in any research situation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060090305
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  • 5
    Electronic Resource
    Electronic Resource
    Foreword (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 1-1 
    Details
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080102
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  • 6
    Electronic Resource
    Electronic Resource
    Mycobacterium Avium-intracellulare complex infections in the acquired immunodeficiency syndrome (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 105-113 
    Details
    ISSN: 0741-0581
    Keywords: Electron microscopy ; Histopathology ; Mycobacterium diagnosis ; Mycobacterium therapy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: This reviw examines an important bacterial infection in acquired immunodeficiency syndrome (AIDS). Despite occasional infections with bacteria such as Streptococcus pneumoniae, Haemophilus influenzae, Salmonella, and Nocardia in patients with AIDS, the primary problems of AIDS and invading bacterial infections center around mycobacteropsos. A unique feature of AIDS has been the common identification of disseminated infections with Mycobacterium avium-intracellulare. The following discussion examines our present understading of this group of organisms and how they interact with the compromised host.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080107
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  • 7
    Electronic Resource
    Electronic Resource
    Hypoxic injury to medullary thick ascending limbs in perfused rat kidneys: Reversible and irreversible phases (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 9 (1988), S. 293-298 
    Details
    ISSN: 0741-0581
    Keywords: Renal medulla ; Isolated perfusion ; Anoxic damage ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The tubular epithelial cells located in the renal medulla are normally working in a hypoxic milieu. In isolated rat kidneys perfused with a cell-free medium, the medullary thick ascending limbs of Henle's loop are selectively and reproducibly injured by the imbalance between oxygen demand and supply in this area. Hypoxic lesions rapidly progress from reversible to irreversible forms of cell damage. Reversible injury consists of chromatin margination and mitochondrial swelling, which can disappear upon restoration of an adequate balance of oxygenation. Irreversible injury consists of nuclear pyknosis and cytoplasmic fragmentation, lesions which persist after re-oxygenation or even progress to cell death. Reversible and irreversible phases of hypoxic injury in this distal tubule segment are comparable to, but different from, those previously defined for the proximal tubule.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060090306
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  • 8
    Electronic Resource
    Electronic Resource
    Three-dimensional reconstruction of cells from serial sections and whole-cell mounts using multilevel contouring of stereo micrographs (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 9 (1988), S. 395-411 
    Details
    ISSN: 0741-0581
    Keywords: Serial thick sections ; HVEM ; Stereoscopic viewing ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A comprehensive computer-graphics-based system (STERECON) is described for tracing and digitizing contours from individual or stereopair electron micrographs. The contours are drawn in parallel planes within the micrographs. Provision is also made for tracing and digitizing in full three-dimensional (3-D) coordinates in any direction along linear structures such as cytoskeletal elements. The stereopair micrographs are viewed in combination with the contours being traced on a graphics terminal monitor. This is done either by projecting original electron micrograph (EM) negatives onto a screen and optically combining these images with contour lines being drawn on the monitor, or by first digitizing the images and displaying them directly on the monitor along with the contour lines. Prior image digitization allows computer enhancement of the structures to be contoured. Correction and alignment routines are included to deal with variable section thickness, section distortion and mass loss, variations in photography in the electron microscope, and terminal screen curvature when combining projected images with contour lines on the monitor. The STERECON system organizes and displays the digitized data from successive sections as a 3-D reconstruction. Reconstructions can be viewed in any orientation as contour stacks with hidden lines removed; as wire-frame models; or as shaded, solid models with variable lighting, transparency, and reflectivity. Volumes and surface areas of the reconstructed objects can be determined. Particular attention was paid to making the system convenient for the biological user. Users are given a choice of three different stereo-viewing methods.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060090406
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  • 9
    Electronic Resource
    Electronic Resource
    Masthead (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 10 (1988) 
    Details
    ISSN: 0741-0581
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060100101
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  • 10
    Electronic Resource
    Electronic Resource
    Image registration in electron microscopy: Application of a robust method (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 10 (1988), S. 27-33 
    Details
    ISSN: 0741-0581
    Keywords: Electron microscopy ; Image processing ; Image registration ; Robustness ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The geometric registration of two electron microscopic images generally is performed by maximizing the cross-correlation coefficient between them. We show that a new similarity measure (the number of sign changes) is useful for performing simultaneously geometric and gray-level registration. This method is robust, which means that it provides a good estimation of the parameters even in the presence of outliers that cannot be described by the registration model.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060100105
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